A rapid microbial detection kit

Abstract

The utility model relates to microorganism detection technical field discloses a kind of microorganism rapid detection kits, to solve the problem of existing detection kits operation cumbersome and easy to pollute detection efficiency low. The kit includes the box and box cover connected by hinge, box body inside is sequentially provided with sampling area reaction area and interpretation area from left to right, disposable sampling swab and the sampling tube of preloaded sample storage liquid are placed in the sampling tool fixed groove of sampling area, the reaction hole plate of pre-coated microorganism specificity antibody is equipped in the upper portion of reaction area, reagent storage groove with easy-to-tear sealing film on the mouth is equipped in the lower portion, light source plate is equipped in the upper portion of interpretation area, comparison card is equipped in the lower portion, battery compartment is loaded in the inside of box body, outside surface is equipped with switch button and display screen. Its integrated sampling reaction interpretation whole process is simple and easy to operate and prevent pollution, light source plate guarantees interpretation accuracy, battery compartment power supply makes use scene more flexible, effectively improve detection efficiency and practicality.

Description

Technical Field

[0001] This utility model relates to the field of microbial detection technology, and in particular to a detection kit that can quickly complete microbial sampling, reaction and result interpretation. Background Technology

[0002] Microbial testing plays a crucial role in medical diagnostics, food safety, and environmental monitoring. Traditional microbial testing kits typically consist of multiple individually packaged reagent tubes, sampling tools, and reaction containers. Each kit requires unpacking and transferring reagents individually, a cumbersome process prone to contamination, resulting in low testing efficiency and lengthy processing times. Furthermore, existing kits lack auxiliary control mechanisms for reaction conditions (such as temperature and time), making it difficult to guarantee testing stability and affecting the accuracy of results.

[0003] Therefore, there is a need to design a rapid microbial detection kit that is compact, easy to operate, and can help improve detection efficiency and stability. Utility Model Content

[0004] The purpose of this invention is to overcome the shortcomings of existing technologies, such as cumbersome operation, easy contamination, and low detection efficiency, and to provide a rapid microbial detection kit. Through optimized structural design, it achieves integrated and rapid operation of sampling, reaction, and interpretation, while improving detection stability.

[0005] To address the aforementioned technical problems, this utility model discloses a rapid microbial detection kit, comprising a box body and a lid, the box body and lid being connected by a hinge; the box body contains, from left to right, a sampling area, a reaction area, and an interpretation area; the sampling area has a sampling tool fixing slot, in which a disposable sampling swab and a sampling tube are placed, the sampling tube being pre-filled with sample preservation solution; the upper part of the reaction area has a reaction plate, the reaction plate being pre-coated with microbial-specific antibodies; the lower part of the reaction area has at least one reagent storage slot, in which a reagent tube pre-filled with detection reagents is placed, the tube opening being covered with an easy-tear sealing film; the upper part of the interpretation area has a light source plate, and the lower part of the interpretation area has a comparison card; The battery compartment is also fixedly installed inside the box. The outer side of the box is equipped with a switch button and a display screen. The battery compartment is used to power the display screen and the light source board, and the switch button is used to control the start and stop of the battery compartment.

[0006] The box lid has an elastic fabric fixedly installed inside, and elastic cotton is filled between the elastic fabric and the box lid to form a truncated cone structure with a raised center. When the box lid is closed, the elastic fabric presses against the reaction zone.

[0007] The box body is provided with a first receiving groove for accommodating the reaction plate, and a temperature sensing element located inside the first receiving groove is also fixedly installed inside the box body; a controller is also fixedly installed inside the box body, and the controller is electrically connected to the temperature sensing element, the display screen, the switch button, the battery compartment, and the light source board.

[0008] The sampling tool fixing slot and the reagent storage slot are both equipped with anti-slip silicone pads on their inner walls.

[0009] A handle is fixedly installed on the outside of the box.

[0010] The bottom of the box is fixedly equipped with anti-slip pads.

[0011] The detection reagents include enzyme-labeled secondary antibody, colorimetric solution, and stop solution, which are pre-packaged in different reagent tubes.

[0012] The box body is provided with a second receiving slot for accommodating the comparison cards; there are multiple comparison cards, which are stacked in the second receiving slot.

[0013] Compared with the prior art, the embodiments of this utility model have the following beneficial effects: (1) The internal structure of the box is arranged in sequence according to the sampling area, reaction area and interpretation area, integrating the full process of sampling, reaction and interpretation. No additional tools or sample transfer are required, reducing operation steps and improving detection efficiency.

[0014] (2) The sampling tube is pre-filled with sample preservation solution and the reagent tube is pre-filled with test reagents and the tube opening is equipped with an easy-tear sealing film to avoid contamination of the sample and reagents before use. At the same time, the reagent dispensing step is eliminated, simplifying the operation process.

[0015] (3) The light source board in the interpretation area provides stable lighting conditions, which, together with the comparison card, enables quick and intuitive interpretation, reduces the influence of ambient light on the result judgment, and improves the accuracy of the test results.

[0016] (4) The battery compartment powers the display screen and the light source board. The switch button controls the start and stop of the battery compartment. It does not rely on an external power source, making the usage scenarios more flexible. The display screen can help present detection-related information, enhancing the ease of use. Attached Figure Description

[0017] To more clearly illustrate the technical solutions in the embodiments of this utility model, the drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0018] Figure 1 This is a schematic diagram of the structure of the rapid microbial detection kit in this embodiment. Detailed Implementation

[0019] To enable those skilled in the art to better understand the present invention, the technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0020] The terms "first," "second," etc., in the specification, claims, and accompanying drawings of this utility model are used to distinguish different objects, not to describe a specific order. Furthermore, the terms "comprising" and "having," and any variations thereof, are intended to cover non-exclusive inclusion. For example, a process, method, apparatus, product, or end that includes a series of steps or units is not limited to the listed steps or units, but may optionally include steps or units not listed, or may optionally include other steps or units inherent to these processes, methods, products, or ends.

[0021] In this document, the term "embodiment" means that a particular feature, structure, or characteristic described in connection with an embodiment may be included in at least one embodiment of the present invention. The appearance of this phrase in various places throughout the specification does not necessarily refer to the same embodiment, nor is it a separate or alternative embodiment mutually exclusive with other embodiments. It will be explicitly and implicitly understood by those skilled in the art that the embodiments described herein can be combined with other embodiments.

[0022] This utility model discloses a specific implementation of a rapid microbial detection kit. Please see [link to kit]. Figure 1 The kit includes a box body 11 and a lid 12 connected by a hinge 14. Inside the box body 11, from left to right, are a sampling area 21, a reaction area 22, and an interpretation area 23. The sampling area 21 has a sampling tool fixing slot 33 containing a disposable sampling swab 31 and a sampling tube 32 pre-filled with sample preservation solution. The reaction area 22 has a reaction plate 41 pre-coated with microbial-specific antibodies at the top and a reagent storage slot with pre-filled test reagents and an easy-tear sealing film at the tube opening at the bottom. The interpretation area 23 has a light source plate 52 at the top and a comparison card 51 at the bottom. The box body 11 contains a battery compartment, and the outer side has a switch button 54 and a display screen 53. It integrates the entire sampling, reaction, and interpretation process, making operation simple and preventing contamination. The light source plate 52 ensures interpretation accuracy, and the battery compartment provides power, making it more flexible in various application scenarios and effectively improving detection efficiency and practicality.

[0023] This novel rapid microbial detection kit includes a box body 11 and a lid 12, which are connected by a hinge 14. A handle 15 is fixedly installed on the outside of the box body 11 for easy carrying of the kit to different testing scenarios. Anti-slip pads 16 are fixedly installed on the bottom of the box body 11 to increase the friction between the kit and the placement surface, preventing slippage during use and improving overall placement stability. An elastic fabric 13 is fixedly installed on the inside of the lid 12, and elastic cotton is filled between the elastic fabric 13 and the lid 12 to form a truncated cone structure with a raised center. This structure utilizes the pressure generated by elastic deformation to press the reaction zone 22 when the lid 12 is closed, preventing the reaction well plate 41 and reagent tube 43 from shaking during transportation or use, which could lead to reagent leakage or sample spillage, while also reducing interference from the external environment on the reaction process. In other embodiments, the elastic fabric 13 inside the lid 12 can also be replaced with an elastic silicone pad 34. The elastic silicone pad 34 and the lid 12 are filled with elastic sponge, which can also form a truncated cone structure with a raised center, so as to achieve the effect of pressing the reaction zone 22 when closed.

[0024] The internal structure of the housing 11 comprises, from left to right, a sampling area 21, a reaction area 22, and an interpretation area 23. This integrated layout combines the entire process of sampling, reaction, and interpretation, eliminating the need for additional tools or sample transfer, reducing operational steps, and effectively improving testing efficiency. The sampling area 21 features a sampling tool fixing slot 33, with an anti-slip silicone pad 34 on its inner wall. This increases contact friction to secure the disposable sampling swab 31 and sampling tube 32, preventing them from shifting or colliding within the slot and further enhancing stability. The sampling tube 32 is pre-filled with sample preservation solution, which can be prepared on-site when not in use. This simplifies the operation process while protecting sample activity and preventing sample deterioration from affecting test results.

[0025] The upper part of the reaction zone 22 is provided with a first receiving groove for accommodating the reaction plate 41. The reaction plate 41 is pre-coated with microbial-specific antibodies, which can specifically bind to the target microorganisms in the sample, providing a basis for specific recognition in subsequent detection. A temperature sensing element 42 located inside the first receiving groove is also fixedly installed inside the housing 11. The temperature sensing element 42 can monitor the temperature of the reaction plate 41 in real time, ensuring that the reaction proceeds at a suitable temperature and avoiding the impact of temperature fluctuations on the binding efficiency of the antibody and the target microorganism. A controller is also fixedly installed inside the housing 11. The controller is electrically connected to the temperature sensing element 42, the display screen 53, the switch button 54, the battery compartment, and the light source board 52. The controller can transmit the temperature data detected by the temperature sensing element 42 to the display screen 53, allowing the user to clearly understand the reaction conditions and improve the controllability of the detection. In other embodiments, the temperature sensing element 42 can also be replaced by a temperature sensor. The temperature sensor has higher detection accuracy and can more accurately monitor the reaction temperature, further ensuring the accuracy of the detection results. At least one reagent storage tank is located at the bottom of the reaction zone 22. The inner wall of the tank is also lined with an anti-slip silicone pad 34. Pre-filled reagent tubes 43 contain pre-packaged test reagents, including enzyme-labeled secondary antibody, chromogenic solution, and stop solution, each pre-packaged in a separate reagent tube 43 for easy access and to prevent reagent mixing from affecting the test. The openings of the reagent tubes 43 are equipped with easy-tear sealing films to effectively isolate external contamination. Reagents can be accessed simply by tearing open the sealing film, eliminating the cumbersome step of reagent dispensing and preventing contamination before use, thus ensuring the reliability of the test results.

[0026] The upper part of the interpretation area 23 is provided with a light source plate 52, and the lower part is provided with a second receiving slot. The second receiving slot is used to hold multiple comparison cards 51, which are stacked in the second receiving slot. Users can select the corresponding comparison card 51 according to the different target microorganisms, thus expanding the applicability of the reagent kit. The light source plate 52 can provide stable lighting conditions, avoiding interference from excessively strong or weak ambient light on result interpretation, making result comparison clearer and more intuitive, and improving the accuracy of the test results. In other embodiments, the comparison card 51 can also be replaced with a plastic card with a standard color scale. Plastic cards have stronger stain resistance, longer service life, and can still meet the needs of result comparison. The battery compartment is also fixedly installed inside the box body 11. The outer side of the box body 11 is provided with a switch button 54 and a display screen 53. The battery compartment is used to power the display screen 53 and the light source plate 52, eliminating the need for an external power source, making the use of the reagent kit more flexible, and convenient to use in both laboratory and field testing. The switch button 54 is used to control the start and stop of the battery compartment, while the display screen 53 can display real-time detection-related information such as temperature and power supply status, further enhancing the ease of use.

[0027] In other embodiments, the detection reagents can be replaced with corresponding specific detection reagents, such as enzyme-labeled antibodies or chromogenic substrates for different bacteria or viruses, depending on the target microorganism, to adapt to more detection scenarios. The electrical connection between the controller and each component realizes the integration of temperature monitoring, power supply control, and information display. The principle is that the controller receives the signal from the temperature sensing element 42, converts it into digital information, and then transmits it to the display screen 53. At the same time, it responds to the instructions of the switch button 54 to control the power supply of the battery compartment, ensuring that each component works together and improving the level of intelligence in detection.

[0028] The usage procedure for this reagent kit is as follows: When using the kit, carry it to the testing site using the handle 15, and place it stably on the operating surface using the anti-slip pads 16 at the bottom. Open the lid 12; the elastic fabric 13 will return to its original shape after losing pressure. Take out the disposable sampling swab 31 from the sampling tool fixing slot 33 in the sampling area 21 to collect the target sample. After collection, place the sampling swab 31 into the sampling tube 32 pre-filled with sample preservation solution, and stir thoroughly to dissolve the sample in the preservation solution. The sample preservation solution maintains the activity of microorganisms in the sample, preventing sample deterioration before testing. Then, remove the reagent tubes 43 from the reagent storage tank at the bottom of the reaction area 22, tear open the easy-tear sealing film at the tube opening, and add the sample solution from the sampling tube 32 into the reaction plate 41 in the first receiving slot at the top of the reaction area 22. The pre-coated microbial-specific antibodies in the reaction plate 41 specifically bind to the target microorganisms in the sample. At this time, the temperature sensor 42 monitors the temperature of the reaction plate 41 in real time, and the controller transmits the temperature data to the display screen 53. The user observes the temperature changes through the display screen 53 to ensure that the reaction is carried out at a suitable temperature and to avoid the effect of improper temperature on the antigen-antibody binding effect. After the incubation is completed, the enzyme-labeled secondary antibody and the chromogenic solution in the reagent tube 43 are added in sequence. Washing is performed as needed after each step. The enzyme-labeled secondary antibody specifically binds to the bound antigen-antibody complex, and the chromogenic solution undergoes a color reaction under the catalysis of the enzyme. After the reaction has been carried out for a period of time, the stop solution is added to terminate the reaction. Press the switch button 54 on the outside of the box 11, and the battery compartment supplies power to the light source plate 52. The light source plate 52 emits stable light. The user takes out the corresponding comparison card 51 from the second accommodating slot in the interpretation area 23 and compares the color of the reaction plate 41 with that of the comparison card 51. The test result is judged according to the color intensity. After the test is completed, press the switch button 54 again to turn off the power, close the box cover 12, and the elastic fabric 13 forms a truncated cone structure with a raised center under the pressure of the box cover 12, which presses the reaction plate 41 and reagent tube 43 of the reaction zone 22 to facilitate subsequent transportation or storage.

[0029] Finally, it should be noted that the microbial rapid detection kit disclosed in this utility model embodiment is only a preferred embodiment of this utility model and is only used to illustrate the technical solution of this utility model, not to limit it. Although this utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the various embodiments of this utility model.

Claims

1. A rapid microbial detection kit, comprising a box body and a lid, wherein the box body and the lid are connected by a hinge; characterized in that: The box contains, from left to right, a sampling area, a reaction area, and an interpretation area. The sampling area is equipped with a sampling tool fixing slot, in which disposable sampling swabs and sampling tubes are placed, and the sampling tubes are pre-filled with sample preservation solution; The upper part of the reaction zone is provided with a reaction plate, and the reaction plate is pre-coated with microbial-specific antibodies; the lower part of the reaction zone is provided with at least one reagent storage tank, and the reagent storage tank contains a reagent tube pre-filled with detection reagents, and the opening of the reagent tube is provided with an easy-tear sealing film. The upper part of the interpretation area is provided with a light source plate, and the lower part of the interpretation area is provided with a comparison card; The box also has a battery compartment fixedly installed inside. The outer side of the box is provided with a switch button and a display screen. The battery compartment is used to power the display screen and the light source board. The switch button is used to control the start and stop of the battery compartment.

2. The rapid microbial detection kit according to claim 1, characterized in that: An elastic fabric is fixedly installed on the inside of the box lid, and elastic cotton is filled between the elastic fabric and the box lid so that the elastic fabric forms a truncated cone structure with a raised center. When the box lid is closed, the elastic fabric presses against the reaction zone.

3. The rapid microbial detection kit according to claim 1, characterized in that: The box body is provided with a first receiving groove for accommodating the reaction plate, and a temperature sensing element located inside the first receiving groove is also fixedly installed inside the box body; a controller is also fixedly installed inside the box body, and the controller is electrically connected to the temperature sensing element, the display screen, the switch button, the battery compartment, and the light source board respectively.

4. The rapid microbial detection kit according to claim 1, characterized in that: The inner walls of the sampling tool fixing slot and the reagent storage slot are both equipped with anti-slip silicone pads.

5. The rapid microbial detection kit according to claim 1, characterized in that: A handle is fixedly installed on the outside of the box.

6. The rapid microbial detection kit according to claim 1, characterized in that: The bottom of the box is fixedly equipped with anti-slip pads.

7. The rapid microbial detection kit according to claim 1, characterized in that: The detection reagents include enzyme-labeled secondary antibody, colorimetric solution, and stop solution, which are pre-packaged in different reagent tubes.

8. The rapid microbial detection kit according to claim 1, characterized in that: The box body is provided with a second receiving slot for accommodating the comparison card; there are multiple comparison cards, and the multiple comparison cards are stacked in the second receiving slot.

Images