Optical adjustment device and laser confocal scanning microscope

Abstract

The utility model discloses an optical adjusting device and laser confocal scanning microscope, wherein, optical adjusting device includes: adjusting frame is equipped with first light -through port for laser shooting in or shooting on adjusting frame, and adjusting base is slidably assembled in the adjusting frame along the vertical direction, and adjusting frame is equipped with height adjusting mechanism for driving adjusting base lifting movement, and the light height adjusting lens barrel is borne on adjusting base, and the second light -through port is equipped on the light height adjusting lens barrel, and the deflection adjusting panel is rotatably assembled in the light height adjusting lens barrel, and the pitch adjusting board is hinged in the deflection adjusting panel and is located in the light height adjusting lens barrel, and the reflector is equipped on the pitch adjusting board for reflecting laser, and the lock piece group for limiting pitch adjusting board in the predetermined pitch angle, the utility model integrates height adjustment, deflection angle adjustment and pitch angle adjustment, and the whole structure is simpler and more easily realized, and the cost is lower.

Description

Technical Field

[0001] This utility model relates to the field of scanning microscope technology, and in particular to an optical adjustment device and a laser confocal scanning microscope. Background Technology

[0002] A confocal laser scanning microscope (CLSM) is a modern biomedical imaging instrument. It adds a laser scanning device to fluorescence microscopy, using ultraviolet or visible light to excite fluorescent probes. It employs a laser as the scanning light source, rapidly scanning point-by-point, line-by-line, and plane-by-plane to create an image. The scanning laser and fluorescence collection share a single objective lens; the focal point of the objective lens is also the focal point of the scanning laser and the instantaneous image point. The system focuses once, limiting the scan to a single plane of the sample. By adjusting the focusing depth, images of different depths of the sample can be obtained. This image information is stored in a computer, and through computer analysis and simulation, the three-dimensional structure of the cell sample can be displayed.

[0003] In the process of laser confocal scanning imaging, pitch, yaw and height need to be adjusted due to optical requirements. Currently, different adjustment modules are needed to achieve different adjustment purposes, which are complex and costly. How to achieve simple and convenient adjustment has become an urgent problem to be solved. Utility Model Content

[0004] In order to overcome the shortcomings of the prior art, the purpose of this utility model is to provide an optical adjustment device and a laser confocal scanning microscope, which has the advantages of integrating multiple adjustment methods, simple structure and low cost.

[0005] The objective of this utility model is achieved through the following technical solution: According to a first aspect of the present disclosure, an optical adjustment device is provided, comprising: An adjustment frame is provided with a first light-transmitting port for laser to enter or exit; An adjustment base is slidably mounted on the adjustment frame in the vertical direction, and the adjustment frame is provided with a height adjustment mechanism for driving the adjustment base to move up and down; A height-adjusting lens barrel supported on the adjustment base, wherein the height-adjusting lens barrel is provided with a second light-transmitting port corresponding to the first light-transmitting port; A sway adjustment panel is rotatably mounted on the optical height adjustment lens barrel; A pitch adjustment plate hinged to the yaw adjustment panel and located within the optical height adjustment lens barrel, the pitch adjustment plate being provided with a reflector for reflecting laser light; and, A locking assembly for limiting the pitch adjustment plate to a predetermined pitch angle.

[0006] To achieve the above technical solution, during use, the laser is input into the height-adjusting lens barrel through the first and second light-passing ports. After being reflected by the reflector, it is emitted from the first and second light-passing ports for laser confocal scanning. The height of the height-adjusting lens barrel is adjusted by driving the adjustment base to move up and down through the height adjustment mechanism. By rotating the yaw adjustment panel, the pitch adjustment plate can be driven to deflect synchronously, thereby adjusting the deflection angle of the reflector. By turning the locking component, the pitch adjustment plate is restricted to different positions, thereby changing the pitch angle of the reflector. Through the above structure, height adjustment, deflection angle adjustment, and pitch angle adjustment are integrated, and the overall structure is simpler, easier to implement, and lower in cost.

[0007] In some exemplary embodiments, the height adjustment mechanism includes an adjustment screw rotatably mounted on the adjustment frame and threadedly connected to the adjustment base, the adjustment screw driving the adjustment base to move up and down by forming a threaded drive with the adjustment base.

[0008] To achieve the above technical solution, the height of the optical height adjustment lens barrel is adjusted by rotating the adjusting screw to form a threaded transmission with the adjusting base, thereby driving the adjusting base to move up and down.

[0009] In some exemplary embodiments, the adjusting screw is further provided with an operating part.

[0010] The above technical solution is implemented to facilitate the operation of the rotating adjusting screw.

[0011] In some exemplary embodiments, the yaw adjustment panel is provided with an adjustment slot, and an adjustment locking member threadedly connected to the optical height adjustment lens barrel passes through the adjustment slot.

[0012] To achieve the above technical solution, the yaw adjustment panel can be provided with a certain yaw angle by adjusting the slot, and then the yaw adjustment panel can be locked by tightening the adjustment locking part.

[0013] In some exemplary embodiments, a pitch positioning plate is provided on the side of the pitch adjustment plate near the yaw adjustment panel. The locking assembly includes an upper locking member and a lower locking member arranged symmetrically. The upper locking member is threaded to the yaw adjustment panel and abuts against the upper side of the pitch positioning plate to restrict the pitch adjustment plate from tilting upward. The lower locking member is threaded to the yaw adjustment panel and abuts against the lower side of the pitch positioning plate to restrict the pitch adjustment plate from tilting downward.

[0014] To achieve the above technical solution, the pitch adjustment plate can be positioned at different pitch angles by screwing the upper and lower locking parts into the pitch positioning plate to different depths and pressing them against the pitch positioning plate.

[0015] In some exemplary embodiments, the pitch adjustment plate is provided with a mounting groove, and the outer periphery of the mounting groove is provided with a plurality of glue injection grooves, and the reflector is embedded in the mounting groove.

[0016] To achieve the above technical solution, after the reflector is embedded in the mounting groove, glue is injected into the mounting groove through the glue injection groove to glue and fix the reflector.

[0017] In some exemplary embodiments, the light height adjustment lens barrel is snapped onto the adjustment base, and a tension spring is provided between the light height adjustment lens barrel and the adjustment base to lock the light height adjustment lens barrel.

[0018] To achieve the above technical solution, the light height adjustment lens barrel and the adjustment base are tightened and fixed relative to each other by a tension spring, which simplifies the installation process and facilitates disassembly and maintenance.

[0019] In some exemplary embodiments, the light height adjustment lens barrel is provided with a first pin, the adjustment base is provided with a second pin, and the two ends of the tension spring are respectively engaged with the first pin and the second pin.

[0020] The above technical solution enables the installation and fixation of the tension spring.

[0021] In some exemplary embodiments, the bottom of the adjustment frame is provided with a mounting base plate, and the mounting base plate is provided with a plurality of connection lock holes.

[0022] Implementing the above technical solution facilitates locking and fixing the adjustment frame.

[0023] According to a second aspect of the present disclosure, a laser confocal scanning microscope is provided, including the optical adjustment device as described in the first aspect.

[0024] In summary, compared with the prior art, this utility model has the following beneficial effects: This utility model provides an optical adjustment device and a laser confocal scanning microscope. The optical adjustment device includes: an adjustment frame with a first light-transmitting port for laser light to enter or exit; an adjustment base slidably mounted on the adjustment frame in a vertical direction, with a height adjustment mechanism for driving the adjustment base to move up and down; a height-adjusting microscope tube supported on the adjustment base, with a second light-transmitting port corresponding to the first light-transmitting port; a tilt adjustment panel rotatably mounted on the height-adjusting microscope tube; a pitch adjustment plate hinged to the tilt adjustment panel and located within the height-adjusting microscope tube, with a reflector for reflecting laser light; and a locking assembly for limiting the pitch adjustment plate to a predetermined pitch angle. In use, the laser is input into the height-adjusting lens barrel through the first and second light-pass ports. After being reflected by the reflector, it is emitted from the first and second light-pass ports for laser confocal scanning. The height of the lens barrel is adjusted by driving the adjustment base to move up and down through the height adjustment mechanism. The tilt adjustment plate can be tilted synchronously by rotating the tilt adjustment panel to adjust the tilt angle of the reflector. The tilt adjustment plate can be locked in different positions by turning the locking component, thereby changing the tilt angle of the reflector. Through the above structure, height adjustment, tilt angle adjustment and tilt angle adjustment are integrated, and the overall structure is simpler and easier to implement, and the cost is lower. Attached Figure Description

[0025] Figure 1 This is a schematic diagram of the optical adjustment device according to an embodiment of the present invention.

[0026] Figure 2 This is a schematic diagram of the assembly structure of the light height adjustment lens barrel and the adjustment base in an embodiment of this utility model.

[0027] Figure 3 This is a schematic diagram of the structure of the light height adjustment lens tube in an embodiment of this utility model.

[0028] Figure 4 This is a schematic diagram of the assembly structure of the yaw adjustment panel and the pitch adjustment plate in an embodiment of this utility model.

[0029] The numbers and letters in the diagram represent the names of the corresponding components: 10. Adjustment frame; 11. First light-transmitting port; 12. Mounting base; 13. Connecting lock hole; 20. Adjustment base; 21. Adjustment screw; 22. Operating part; 23. Second pin; 30. Light height adjustment lens barrel; 31. Second light-transmitting port; 32. Tension spring; 33. First pin; 40. Yaw adjustment panel; 41. Adjustment slot; 42. Adjustment locking part; 50. Pitch adjustment plate; 51. Reflector; 52. Pitch positioning plate; 53. Mounting slot; 54. Glue injection slot; 60. Locking assembly; 61. Upper locking part; 62. Lower locking part. Detailed Implementation

[0030] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0031] like Figures 1 to 4 As shown, this utility model embodiment provides an optical adjustment device, including: an adjustment frame 10, on which a first light-transmitting port 11 for laser to enter or exit; an adjustment base 20 slidably mounted on the adjustment frame 10 in a vertical direction, on which a height adjustment mechanism for driving the adjustment base 20 to move up and down is provided; a height adjustment lens barrel 30 supported on the adjustment base 20, on which a second light-transmitting port 31 corresponding to the first light-transmitting port 11 is provided; a tilt adjustment panel 40 rotatably mounted on the height adjustment lens barrel 30; a pitch adjustment plate 50 hinged to the tilt adjustment panel 40 and located within the height adjustment lens barrel 30, on which a reflector 51 for reflecting laser is provided; and a locking assembly 60 for limiting the pitch adjustment plate 50 to a predetermined pitch angle.

[0032] Specifically, a mounting base plate 12 is provided at the bottom of the adjustment frame 10. The mounting base plate 12 has several connecting locking holes 13, which are used to insert bolts or other locking components to fix the adjustment frame 10 to the instrument frame, thereby facilitating the locking and fixing of the adjustment frame 10. The adjustment base 20 and the adjustment frame 10 are slidably connected through a sliding rail and a slider. The height adjustment mechanism includes an adjustment screw 21 that is rotatably mounted on the adjustment frame 10 and threadedly connected to the adjustment base 20. The adjustment screw 21 drives the adjustment base 20 to move up and down by forming a threaded transmission with the adjustment base 20. The adjustment screw 21 can be rotatably connected to the adjustment frame 10 through a bearing. A fine threaded hole is opened in the middle of the adjustment base 20, through which the adjustment screw 21 is threadedly connected to the adjustment base 20. By rotating the adjustment screw 21 to form a threaded transmission with the adjustment base 20, the adjustment base 20 is driven to move up and down, thereby realizing the height adjustment of the optical height adjustment lens barrel 30.

[0033] To facilitate the operation of rotating the adjusting screw 21, an operating part 22 is also provided on the adjusting screw 21. The operating part 22 can be an adjusting plate, an operating handle, etc. Taking the operating part 22 as an example of an adjusting plate, the adjusting plate is a thin circular plate, and the bottom of the adjusting screw 21 passes through the adjusting plate. The adjusting plate is fixed to the adjusting screw 21 by means of bonding, key connection, etc.

[0034] The yaw adjustment panel 40 is provided with an adjustment slot 41. An adjustment locking member 42, which is threadedly connected to the optical height adjustment lens barrel 30, passes through the adjustment slot 41. The adjustment slot 41 is oval in shape. The adjustment locking member 42 is a bolt. Usually, there are two sets of adjustment slots 41 and adjustment locking members 42 symmetrically arranged. The adjustment slot 41 can provide a certain yaw angle for the yaw adjustment panel 40. Then, tightening the adjustment locking member 42 can lock the yaw adjustment panel 40.

[0035] The pitch adjustment plate 50 can be connected to the yaw adjustment panel 40 via a cylindrical connector. A pitch positioning plate 52 is provided on the side of the pitch adjustment plate 50 near the yaw adjustment panel 40. The pitch positioning plate 52 and the yaw adjustment panel 40 need to be spaced at a certain distance to allow the pitch positioning plate 52 to yaw. The locking assembly 60 includes an upper locking member 61 and a lower locking member 62 arranged symmetrically. The upper locking member is threaded to the yaw adjustment panel 40 and abuts against the upper side of the pitch positioning plate 52 to restrict the pitch adjustment plate 50 from tilting upward. The lower locking member is threaded to the yaw adjustment panel 40 and abuts against the lower side of the pitch positioning plate 52 to restrict the pitch adjustment plate 50 from tilting downward.

[0036] By screwing the upper locking member 61 and the lower locking member 62 into different depths and pressing them against the pitch positioning plate 52, the pitch adjustment plate 50 can be positioned at different pitch angles to achieve pitch adjustment. For example, when it is necessary to adjust the tilt angle downward, first screw the lower locking member backward. At this time, the pitch adjustment plate 50 will tilt downward with the lower locking member under the action of gravity. After tilting into place, screw the upper locking member forward and press it against the pitch positioning plate 52 to fix the pitch adjustment plate 50. When it is necessary to adjust the tilt angle upward, first screw the upper locking member backward to the adjustment position, and then screw the lower locking member forward to lift the pitch positioning plate 52 upward until the upper locking member presses against the pitch positioning plate 52 to fix the pitch adjustment plate 50.

[0037] Furthermore, the pitch adjustment plate 50 is provided with a mounting groove 53, and the outer periphery of the mounting groove 53 is provided with several glue injection grooves 54. The reflector 51 is embedded in the mounting groove 53. For example, 3-6 sets of glue injection grooves 54 can be arranged along the circumference of the mounting groove 53. After the reflector 51 is embedded in the mounting groove 53, glue is injected into the mounting groove 53 through the glue injection grooves 54 to glue and fix the reflector 51.

[0038] In this embodiment, the height adjustment lens barrel 30 is snapped onto the adjustment base 20. A tension spring 32 is provided between the height adjustment lens barrel 30 and the adjustment base 20 to lock the height adjustment lens barrel 30. A first pin 33 is provided on the height adjustment lens barrel 30, and a second pin 23 is provided on the adjustment base 20. The two ends of the tension spring 32 are respectively snapped onto the first pin 33 and the second pin 23 to achieve the installation and fixation of the tension spring 32. A pin hole is provided on the height adjustment lens barrel 30, and the first pin 33 is inserted and fixed into the pin hole. The upper end of the tension spring 32 is fastened to the first pin 33. A pin groove is provided at the bottom of the adjustment base 20, and the second pin 23 is embedded in the pin groove. The lower end of the tension spring 32 passes through the pin groove and is fastened to the second pin 23. The height adjustment lens barrel 30 and the adjustment base 20 are tightened and fixed relative to each other by the tension spring 32, thereby simplifying the installation process and facilitating disassembly and maintenance.

[0039] In use, the laser is input into the height-adjustable lens barrel 30 through the first light-passing port 11 and the second light-passing port 31. After being reflected by the reflector 51, it is emitted from the first light-passing port 11 and the second light-passing port 31 for laser confocal scanning. The height of the height-adjustable lens barrel 30 is adjusted by driving the adjustment base 20 to move up and down through the height adjustment mechanism. The tilt adjustment plate 50 can be tilted synchronously by rotating the tilt adjustment panel 40 to adjust the tilt angle of the reflector 51. The tilt adjustment plate 50 can be restricted to different positions by turning the locking component, thereby changing the tilt angle of the reflector 51. Through the above structure, height adjustment, tilt angle adjustment and tilt angle adjustment are integrated, and the overall structure is simpler and easier to implement, with lower cost.

[0040] A second aspect of this utility model provides a laser confocal scanning microscope, including the optical adjustment device as described in the first aspect.

[0041] The above embodiments only illustrate several implementation methods of this utility model, and their descriptions are relatively specific and detailed, but they should not be construed as limiting the scope of the utility model patent. It should be noted that for those skilled in the art, several modifications and improvements can be made without departing from the concept of this utility model. These are all equivalent modifications and improvements made to the above embodiments based on the essential technology of this utility model, and all of these fall within the protection scope of this utility model.

Claims

1. An optical adjustment device, characterized in that, include: An adjustment frame is provided with a first light-transmitting port for laser to enter or exit; An adjustment base is slidably mounted on the adjustment frame in the vertical direction, and the adjustment frame is provided with a height adjustment mechanism for driving the adjustment base to move up and down; A height-adjusting lens barrel supported on the adjustment base, wherein the height-adjusting lens barrel is provided with a second light-transmitting port corresponding to the first light-transmitting port; A sway adjustment panel is rotatably mounted on the optical height adjustment lens barrel; A pitch adjustment plate hinged to the yaw adjustment panel and located within the optical height adjustment lens barrel, the pitch adjustment plate being provided with a reflector for reflecting laser light; and, A locking assembly for limiting the pitch adjustment plate to a predetermined pitch angle.

2. The optical adjustment device according to claim 1, characterized in that, The height adjustment mechanism includes an adjustment screw that is rotatably mounted on the adjustment frame and threadedly connected to the adjustment base. The adjustment screw drives the adjustment base to move up and down by forming a threaded transmission with the adjustment base.

3. The optical adjustment device according to claim 2, characterized in that, The adjusting screw is also equipped with an operating part.

4. The optical adjustment device according to claim 1, characterized in that, The yaw adjustment panel is provided with an adjustment slot, and an adjustment locking member threadedly connected to the optical height adjustment lens barrel passes through the adjustment slot.

5. The optical adjustment device according to claim 1, characterized in that, The pitch adjustment plate has a pitch positioning plate on the side near the yaw adjustment panel. The locking assembly includes an upper locking member and a lower locking member arranged symmetrically. The upper locking member is threaded to the yaw adjustment panel and abuts against the upper side of the pitch positioning plate to restrict the pitch adjustment plate from tilting upward. The lower locking member is threaded to the yaw adjustment panel and abuts against the lower side of the pitch positioning plate to restrict the pitch adjustment plate from tilting downward.

6. The optical adjustment device according to claim 1, characterized in that, The pitch adjustment plate is provided with a mounting groove, and the outer periphery of the mounting groove is provided with several glue injection grooves, and the reflector is embedded in the mounting groove.

7. The optical adjustment device according to claim 1, characterized in that, The light height adjustment lens barrel is snapped into the adjustment base, and a tension spring is provided between the light height adjustment lens barrel and the adjustment base to lock the light height adjustment lens barrel.

8. The optical adjustment device according to claim 7, characterized in that, The light height adjustment lens barrel is provided with a first pin, the adjustment base is provided with a second pin, and the two ends of the tension spring are respectively engaged with the first pin and the second pin.

9. The optical adjustment device according to claim 1, characterized in that, The bottom of the adjustment frame is provided with a mounting base plate, and the mounting base plate is provided with several connection lock holes.

10. A laser confocal scanning microscope, characterized in that, Includes the optical adjustment device as described in any one of claims 1-9.

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