An immune cell cryopreservation device

Abstract

The utility model is suitable for cell cryopreservation device technical field provides an immune cell cryopreservation device, including the rotation disc that can rotate about fixed ring, and the sealed groove and the mouth of taking are set up in rotation disc and top cover corresponding. When needing to find or take specific sample, the sealed groove on it is aligned with the mouth of taking above target test tube through handle rotation rotation disc. At this moment, the sealing plug in sealed groove can be pulled out, and the test tube label is directly observed through transparent glass block on the mouth of taking to confirm, and the target test tube is taken and put operation. The design has realized the sample accurate positioning and individual use under the condition of not opening whole top cover. The utility model greatly reduces the exposure area and time of low temperature environment in the cryopreservation box when sampling, can maximumly restrain the cold quantity escape and temperature rise, effectively avoids the risk that other storage sample in the box is damaged in activity because of temperature violent fluctuation, improves operation efficiency and security.

Description

Technical Field

[0001] This invention belongs to the field of cell cryopreservation device technology, and particularly relates to an immune cell cryopreservation device. Background Technology

[0002] Immunotherapy is an important area of ​​development in biomedicine, and high-quality cell cryopreservation is a crucial prerequisite for successful treatment. Currently, liquid nitrogen cryopreservation is commonly used to preserve immune cells for long periods to maximize cell viability.

[0003] In the prior art, there are various devices for cell cryopreservation. For example, Chinese Patent (Announcement No.: CN215013016U) discloses an immune cell cryopreservation device, which includes a box, a storage container, and a lid. The cell tube is fixed by a cell storage tube formed by an inward indentation at the upper end of the storage container and a limiting metal plate fixed to its inner surface. This design improves the convenience of cell tube fixation to a certain extent.

[0004] However, after practical application and analysis, this type of cryopreservation device still has some shortcomings that urgently need improvement: First, when a specific sample needs to be retrieved, the entire lid often needs to be opened, causing a large area of ​​the cryopreservation container's low-temperature environment to come into contact with the outside environment. This not only causes a large amount of cold energy to dissipate and liquid nitrogen to be consumed rapidly, but also causes drastic temperature fluctuations inside the container, causing irreversible damage to the viability of other unretrieved cell samples. Second, existing fixation methods (such as limiting metal plates) have poor adaptability to test tubes of different diameters, and the fixation effect is limited. During handling or handling, test tubes are prone to tipping over or colliding, posing a risk of sample damage. In addition, when locating a target sample, it is usually necessary to open the storage compartment one by one, which is cumbersome and inefficient.

[0005] Therefore, there is an urgent need for a new type of immune cell cryopreservation device that can achieve rapid and accurate sample positioning and retrieval in different areas, minimize temperature fluctuations, and safely and stably adapt to and protect cryopreservation tubes of different sizes. Utility Model Content

[0006] This invention provides an immune cell cryopreservation device, which aims to solve the problems of large temperature fluctuations when handling test tubes in current cell cryopreservation devices and the inability to properly adapt to and protect cryopreservation tubes of different specifications.

[0007] This invention is achieved as follows: an immune cell cryopreservation device, comprising:

[0008] A cryogenic storage box, wherein an inlet pipe is provided on the outer wall of the cryogenic storage box for transporting liquid nitrogen into the cryogenic storage box for storage;

[0009] The top cover is fixed to the top of the cryogenic container;

[0010] A retaining ring is detachably fixed to the top of the top cover by bolts;

[0011] A rotating disk disposed on the upper side of the fixed ring and capable of rotating relative to the fixed ring;

[0012] A handle fixed to the top of the rotating disk;

[0013] A central column is fixedly connected to the bottom of the cryopreservation box, and the central column extends rotatably to the inside of the rotating disk;

[0014] Multiple clamping components are distributed inside the cryo-box for clamping and limiting the test tubes, and a label is affixed to the top of the test tubes;

[0015] Enclosed components arranged in a ring array on the top cover;

[0016] The top cover has multiple access ports, and the number of access ports is the same as the number of clamping components and they correspond one-to-one, for installing the sealing components.

[0017] Preferably, a T-shaped slip ring is provided on the outer periphery of the rotating disk, and a T-shaped sliding cavity is provided on the upper side of the fixed ring for the T-shaped slip ring to slide.

[0018] Preferably, the rotating disk has several sealing grooves through it, and a sealing plug is inserted into the inside of the sealing groove.

[0019] Preferably, the sealing component includes an overlapping ring fixed to the inner wall of the retrieval port, a sealing cap is fitted into the retrieval port located on the upper side of the overlapping ring, and a rubber ring is provided on the outer periphery of the sealing cap.

[0020] Preferably, the sealing assembly further includes a glass block fixedly sleeved inside the sealing cover, and the top of the sealing cover located on both sides of the glass block is provided with a placement cavity, and a lever is fixedly connected inside each of the two placement cavities.

[0021] Preferably, the glass block is cylindrical, and the diameter of the glass block is larger than the diameter of the top of the test tube.

[0022] Preferably, the clamping assembly includes a base fixed to the bottom of the cryopreservation box, and the upper side of the base has three limiting cavities arranged in a ring array.

[0023] Preferably, the clamping assembly further includes three clamping rods, which are slidably sleeved inside the three limiting cavities, and each of the three clamping rods has a guide end at its top.

[0024] Preferably, a limiting rod is fixedly connected to the inner side of each of the three limiting cavities, and the limiting rod slides through the inside of the clamping rod.

[0025] Preferably, a return spring is sleeved on the outer side of the limiting rod, and the two ends of the return spring are respectively connected to the outer wall of the clamping rod and one end of the limiting cavity.

[0026] Compared with related technologies, the immune cell cryopreservation device provided by this utility model has the following beneficial effects:

[0027] 1. Achieve rapid sample location and retrieval by designated areas, effectively reducing temperature fluctuations: This invention features a rotating disk that can rotate around a fixed ring, with corresponding sealing grooves and retrieval ports on the disk and top cover. When a specific sample needs to be located or retrieved, the rotating disk is rotated by the handle to align the sealing groove with the retrieval port on the target test tube. The sealing plug in the sealing groove can then be removed, and the test tube label can be directly observed through the transparent glass block on the retrieval port for confirmation. Once confirmed, the sealing cap can be opened to retrieve the target test tube. This design enables precise sample location and individual retrieval without opening the entire top cover. Compared to traditional cryopreservation devices that require complete opening of the box lid, this invention significantly reduces the exposure area and time of the low-temperature environment inside the cryopreservation box during sampling, maximizing the suppression of cold loss and temperature rise. This effectively avoids the risk of other stored samples in the box suffering activity loss due to drastic temperature fluctuations, while simultaneously improving operational efficiency and safety.

[0028] 2. Providing adaptive flexible clamping to ensure stable and safe test tube storage: The clamping assembly of this invention consists of a base, three clamping rods with guide ends, a limiting rod, and a return spring. When the test tube is placed downwards, its body first contacts the guide end at the top of the clamping rod. Under pressure, the three clamping rods slide outwards along the limiting rod, compressing the return spring, thereby adaptively creating a space matching the diameter of the test tube and holding it tightly. This clamping structure can adaptively fix test tubes of different diameters, making it widely applicable. The continuous elastic clamping force provided by the return spring ensures that the test tube remains upright and stable during storage and transportation, effectively preventing the test tube from tipping over, colliding, or even breaking due to shaking or tilting, providing important physical safety protection for precious cell samples. Simultaneously, the guide ends make the insertion of the test tube smoother and more convenient. Attached Figure Description

[0029] Figure 1 This is a three-dimensional structural diagram of the present invention;

[0030] Figure 2 This is a cross-sectional structural diagram of the present invention;

[0031] Figure 3 This is a cross-sectional view of the rotating disk of this utility model;

[0032] Figure 4 for Figure 2Enlarged structural diagram at point A in the middle;

[0033] Figure 5 This is a schematic diagram of the clamping component structure of this utility model.

[0034] In the diagram: 1. Cryogenic box; 11. Inlet tube; 2. Top cover; 21. Retrieval port; 3. Fixing ring; 4. Handle; 5. Rotary disc; 51. T-shaped slip ring; 52. Sealing groove; 53. Sealing plug; 6. Test tube; 61. Label; 7. Clamping assembly; 71. Base; 72. Clamping rod; 73. Guide end; 74. Return spring; 75. Limiting rod; 76. Limiting cavity; 8. Central column; 9. Sealing assembly; 91. Overlap ring; 92. Sealing cap; 93. Glass block; 94. Placement cavity; 95. Lever. Detailed Implementation

[0035] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs; the terminology used herein in the specification of the application is for the purpose of describing particular embodiments only and is not intended to be limiting of the application; the terms "comprising" and "having," and any variations thereof, in the specification, claims, and foregoing drawings of this application are intended to cover non-exclusive inclusion. The terms "first," "second," etc., in the specification, claims, or foregoing drawings of this application are used to distinguish different objects, not to describe a particular order.

[0036] In this document, the term "embodiment" means that a particular feature, structure, or characteristic described in connection with an embodiment may be included in at least one embodiment of this application. The appearance of this phrase in various places throughout the specification does not necessarily refer to the same embodiment, nor is it a separate or alternative embodiment mutually exclusive with other embodiments. It will be explicitly and implicitly understood by those skilled in the art that the embodiments described herein can be combined with other embodiments.

[0037] Example 1

[0038] A preferred embodiment of the immune cell cryopreservation device provided by this utility model is, for example... Figures 1 to 5As shown: An immune cell cryopreservation device includes a cryopreservation box 1, with an inlet tube 11 on the outer wall of the cryopreservation box 1 for delivering liquid nitrogen into the cryopreservation box 1 for storage; a top cover 2 fixed to the top of the cryopreservation box 1; a fixing ring 3 detachably fixed to the top of the top cover 2 by bolts; a rotating disk 5 disposed on the upper side of the fixing ring 3 and capable of rotating relative to the fixing ring 3; a handle 4 fixed to the top of the rotating disk 5; a central column 8 fixedly connected to the bottom of the cryopreservation box 1, the central column 8 extending rotatably into the inner side of the rotating disk 5; multiple clamping components 7 distributed inside the cryopreservation box 1 for clamping and limiting test tubes 6, with labels 61 affixed to the top of the test tubes 6; and sealing components 9 arranged in a ring array on the top cover 2; the top cover 2 has multiple access ports 21, the number of access ports 21 being the same as the number of clamping components 7 and corresponding one-to-one, for installing the sealing components 9, thereby sealing the access ports 21 through the sealing components 9.

[0039] In a further preferred embodiment of this utility model, a T-shaped slip ring 51 is provided on the outer periphery of the rotating disk 5, and a T-shaped sliding cavity is provided on the upper side of the fixed ring 3 for the T-shaped slip ring 51 to slide. A plurality of sealing grooves 52 are provided through the rotating disk 5, and sealing plugs 53 are inserted into the inner side of the sealing grooves 52 to seal the sealing grooves 52.

[0040] In this embodiment, the sealing assembly 9 includes an overlapping ring 91 fixed to the inner wall of the retrieval port 21. A sealing cap 92 is fitted into the retrieval port 21 located above the overlapping ring 91, and a rubber ring is provided around the outer periphery of the sealing cap 92. The sealing assembly 9 also includes a glass block 93 fixedly fitted inside the sealing cap 92. Placement cavities 94 are opened on the top of the sealing caps 92 on both sides of the glass block 93, and levers 95 are fixedly connected inside each of the two placement cavities 94. The glass block 93 is cylindrical, and its diameter is larger than the diameter of the top of the test tube 6, which facilitates observation of the top of the test tube 6.

[0041] In this embodiment, the clamping assembly 7 includes a base 71 fixed to the bottom of the cryopreservation box 1, and three limiting cavities 76 arranged in a ring array on the upper side of the base 71. The clamping assembly 7 also includes three clamping rods 72, which are slidably sleeved inside the three limiting cavities 76, and each of the three clamping rods 72 has a guide end 73 at its top. Limiting rods 75 are fixedly connected to the inner side of each of the three limiting cavities 76, and the limiting rods 75 slide through the inside of the clamping rods 72. A return spring 74 is sleeved on the outer side of the limiting rod 75, and the two ends of the return spring 74 are respectively connected to the outer wall of the clamping rod 72 and one end of the limiting cavity 76.

[0042] In this embodiment, when the test tube 6, which is about to be cryopreserved, is placed inside the cryopreservation box 1, the rotating disk 5 is turned by the handle 4. Under the action of the T-shaped slip ring 51, the rotating disk 5 can rotate smoothly at the top of the fixed ring 3. (Reference) Figure 2Rotate the rotating disk 5 so that one of its sealing grooves 52 is aligned with the retrieval port 21 directly above the target test tube 6, thus aligning the central axes of the sealing groove 52, retrieval port 21, and target clamping assembly 7. Then, remove the sealing plug 53 from the sealing groove 52. Next, observe the label 61 of the test tube 6 below through the transparent glass block 93 in the center of the sealing cap 92 for final confirmation. After confirmation, pull the sealing cap 92 out of the retrieval port 21 using the lever 95 to operate on the test tube 6. When searching for a sample, it is not necessary to remove the sealing plug 53; the label 61 can be directly observed through the glass block 93 of the sealing cap 92 on each retrieval port 21. By rotating the rotating disk 5 using the handle 4, the label 61 information of all test tubes 6 can be quickly browsed for rapid positioning.

[0043] In this embodiment, reference Figure 5 Under the action of the guide end 73, the test tube 6 is easily inserted between the three clamping rods 72, allowing the bottom of the guide end 73 to slide stably along the inner side of the limiting cavity 76, causing the three clamping rods 72 to move away from each other, thus adaptively clamping the test tube 6. As the three clamping rods 72 move away from each other, the return spring 74 is gradually compressed. Utilizing the good elasticity of the return spring 74, the test tube 6 can be stably clamped by the clamping rods 72.

[0044] In this embodiment, a suitable number of test tubes 6 are installed inside the cryopreservation box 1 using the method described above. Then, the sealing cap 92 is re-sleeved inside the retrieval port 21 and overlapped on the upper end of the overlapping ring 91. Next, the sealing plug 53 is sleeved inside the sealing groove 52 for further sealing. Then, liquid nitrogen is introduced into the cryopreservation box 1 through the inlet pipe 11 for freezing and storage.

[0045] In this embodiment, when a specific test tube 6 needs to be removed, firstly, the rotating disk 5 is rotated using the handle 4 to align the sealing groove 52 with the target retrieval opening 21. Next, the sealing plug 53 in the sealing groove 52 is pulled out. At this point, the operator can observe the sealing cap 92 below and the label 61 on the test tube 6 through the empty sealing groove 52. By rotating the rotating disk 5, different sealing caps 92 can be pierced through the sealing groove 52. Since the glass block 93 is made of transparent glass, the label 61 on the top of the test tube 6 located below it can be observed through the glass block 93. When the cryo-box 1 is not the cryo-box 1 that needs to be taken out, the rotating disk 5 can be rotated until the sealing groove 52 is above the cryo-box 1 to be taken out. At this time, the test tube 6 can be pulled out from the clamping assembly 7 by pulling the sealing cap 92 out of the retrieval port 21. There is no need to open the entire top cover of the cryo-box 1, which significantly reduces the degree of temperature fluctuation inside the top cover 2 and avoids interference with other cryo-boxes 1.

[0046] It is worth noting that the circuits, electronic components, and modules involved in this utility model are all existing technologies, which can be fully implemented by those skilled in the art, and need not be elaborated upon. The content protected by this utility model does not involve any improvement to the software and methods.

[0047] It should be understood that the disclosed apparatus can be implemented in other ways, as illustrated in the embodiments provided in this application. For example, the apparatus embodiments described above are merely illustrative; for instance, the division of units described above is only a logical functional division of an immune cell cryopreservation apparatus. In actual implementation, there may be other division methods. For example, multiple units or components may be combined or integrated into another system, or some features may be ignored or not executed. Furthermore, the coupling or communication connections shown or discussed may be through some interfaces; the indirect coupling or communication connections between devices or units may be telecommunications or other forms.

[0048] The above embodiments are only used to illustrate the technical solutions of this utility model, and are not intended to limit the scope of protection of this utility model. Obviously, the described embodiments are only some embodiments of this utility model, not all embodiments. Based on these embodiments, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this utility model. Although this utility model has been described in detail with reference to the above embodiments, those skilled in the art can still combine, add, delete, or otherwise adjust the features of the various embodiments of this utility model according to the circumstances without conflict or creative effort, thereby obtaining different technical solutions that do not fundamentally depart from the concept of this utility model. These technical solutions are also within the scope of protection of this utility model.

Claims

1. An immune cell cryopreservation device, characterized in that, include: A cryogenic chamber (1) is provided with an inlet pipe (11) on the outer wall of the cryogenic chamber (1) for transporting liquid nitrogen into the cryogenic chamber (1) for storage; The top cover (2) is fixed to the top of the cryogenic box (1); A retaining ring (3) is detachably fixed to the top of the top cover (2) by bolts; A rotating disk (5) is disposed on the upper side of the fixed ring (3) and is capable of rotating relative to the fixed ring (3); A handle (4) is fixed to the top of the rotating disk (5); A central column (8) is fixedly connected to the bottom of the cryopreservation box (1), and the central column (8) extends rotatably to the inside of the rotating disk (5); Multiple clamping components (7) are distributed inside the cryo-box (1) for clamping and limiting the test tube (6), and a label (61) is affixed to the top of the test tube (6); The sealing components (9) are arranged in a ring array on the top cover (2); The top cover (2) has multiple openings (21) through it. The number of openings (21) is the same as the number of clamping components (7) and they correspond one-to-one. They are used to install the sealing component (9).

2. The immune cell cryopreservation device as described in claim 1, characterized in that, The rotating disk (5) is provided with a T-shaped slip ring (51) on its outer periphery, and the fixed ring (3) has a T-shaped sliding cavity on its upper side for the T-shaped slip ring (51) to slide.

3. The immune cell cryopreservation device as described in claim 2, characterized in that, A plurality of sealing grooves (52) are provided through the rotating disk (5), and a sealing plug (53) is inserted inside the sealing groove (52).

4. The immune cell cryopreservation device as described in claim 3, characterized in that, The sealing component (9) includes an overlapping ring (91) fixed to the inner wall of the retrieval port (21), and a sealing cap (92) is sleeved in the retrieval port (21) located on the upper side of the overlapping ring (91), and a rubber ring is provided on the outer periphery of the sealing cap (92).

5. The immune cell cryopreservation device as described in claim 4, characterized in that, The sealing assembly (9) also includes a glass block (93) fixedly sleeved inside the sealing cover (92). The top of the sealing cover (92) on both sides of the glass block (93) is provided with a placement cavity (94), and a lever (95) is fixedly connected inside the two placement cavities (94).

6. The immune cell cryopreservation device as described in claim 5, characterized in that, The glass block (93) is cylindrical, and the diameter of the glass block (93) is larger than the top diameter of the test tube (6).

7. The immune cell cryopreservation device as described in claim 6, characterized in that, The clamping assembly (7) includes a base (71) fixed to the bottom of the cryopreservation box (1), and the upper side of the base (71) has three limiting cavities (76) arranged in a ring array.

8. The immune cell cryopreservation device as described in claim 7, characterized in that, The clamping assembly (7) further includes three clamping rods (72), which are slidably sleeved inside the three limiting cavities (76), and each of the three clamping rods (72) is provided with a guide end (73) at its top.

9. The immune cell cryopreservation device as described in claim 8, characterized in that, Each of the three limiting cavities (76) is fixedly connected to a limiting rod (75), which slides through the inside of the clamping rod (72).

10. The immune cell cryopreservation device as described in claim 9, characterized in that, A reset spring (74) is sleeved on the outside of the limiting rod (75), and the two ends of the reset spring (74) are respectively connected to the outer wall of the clamping rod (72) and one end of the limiting cavity (76).

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