Portable device for long-term cryopreservation of sika deer diluted semen

Abstract

The utility model discloses a portable cervus nippon dilute semen low temperature long-acting storage device, and the core of the device is that the batch storage, low temperature preservation and fine subpackaging function are integrated in a portable incubator, the inside of the storage box is divided into semen storage area and semen subpackaging area through the partition, respectively places dilute semen preservation test tube and the subpackaging fine tube of waiting to fill, and the refrigeration circulating mechanism is integrated in the storage box, and the closed cold air circulation is constituted through the refrigerator and circulating fan, realizes accurate thermostatic low temperature preservation, and semen transfer is connected negative pressure suction head and injection head through a pump body. The device realizes the full process integration of from storage to subpackaging, low temperature operation, and greatly guarantees semen activity and safety.

Description

Technical Field

[0001] This utility model relates to the technical field of semen preservation devices, specifically a portable, low-temperature, long-term preservation device for diluted semen from sika deer. Background Technology

[0002] As an important economic animal, the artificial insemination technology of sika deer plays a key role in the breeding and genetic improvement of superior breeds. In this technology system, semen dilution and low-temperature preservation are important links to ensure semen vitality and extend its effective use time.

[0003] Currently, the industry typically uses test tubes for initial storage of diluted semen, and then dispenses it into thin tubes before use to facilitate insemination. However, existing storage and dispensing equipment is often single-function, with the storage and dispensing processes separated, requiring the transfer of semen samples between different containers or devices during operation. This process has obvious drawbacks: on the one hand, frequent transfers increase the risk of semen sample contamination; on the other hand, it is difficult to maintain a stable low-temperature environment during the transfer process, which can easily cause the semen to experience temperature fluctuations, thus adversely affecting its viability and preservation effect. In addition, existing preservation devices are mostly simple insulated boxes with external cold sources (such as ice packs), which have problems such as inaccurate temperature control, poor uniformity, short battery life, and inability to perform convenient dispensing operations in the field or mobile scenarios. Utility Model Content

[0004] In view of the above-mentioned shortcomings in the existing technology, the purpose of this utility model is to provide a storage device that can store and dispense diluted semen in batches and achieve low-temperature preservation.

[0005] The technical solution adopted by this utility model to achieve the above-mentioned objective is: a portable low-temperature long-term preservation device for diluted semen of sika deer, including a preservation box, a semen preservation test tube, and a dispensing capillary tube. A storage area is provided on one side of the preservation box. A test tube rack and a capillary tube rack are fixedly connected in the storage area. Multiple sets of the semen preservation test tubes are placed on the test tube rack, and multiple sets of the dispensing capillary tubes are placed on the capillary tube rack. A cover is rotatably connected to the preservation box corresponding to the storage area. The cover and the preservation box are connected by a locking buckle. A pump body is fixedly connected inside the storage box. A negative pressure suction head is fixedly connected to the inlet of the pump body through a first conduit. The negative pressure suction head is used in conjunction with the diluted semen storage tube. A filling head is fixedly connected to the outlet of the pump body through a second conduit. The filling head is used in conjunction with the dispensing capillary tube. The storage box is equipped with a refrigeration circulation mechanism, which is connected to the pipeline of the storage area.

[0006] In the above technical solution, the storage box is further provided with a thin tube storage cavity, and the storage box is provided with a take-out port communicating with the thin tube storage cavity. A sealing plate is slidably connected to the storage box, and the sealing plate closes the take-out port.

[0007] In the above technical solution, the diluted semen preservation test tube includes a test tube body and a test tube stopper cap that blocks the opening of the test tube body. The test tube stopper cap has a test tube opening, and the test tube opening is blocked by a small test tube stopper.

[0008] In the above technical solution, the dispensing cap includes a capping tube body and a capping tube plug that blocks the opening of the capping tube body. The capping tube plug has an opening, and the opening is plugged with a small plug.

[0009] In the above technical solution, a suction tip plug is fixedly connected to the negative pressure suction head. When the negative pressure suction head is inserted into the test tube body through the test tube opening, the suction tip plug blocks the test tube opening.

[0010] In the above technical solution, an injection plug is fixedly connected to the injection head. When the injection head is inserted into the thin tube body through the thin tube opening, the injection plug blocks the thin tube opening.

[0011] In the above technical solution, a partition is fixedly connected in the storage area. One side of the partition is a semen storage area, and the other side is a semen dispensing area. The test tube rack is fixedly connected in the semen storage area, and the thin tube rack is fixedly connected in the semen dispensing area. An air vent is provided on the partition.

[0012] In the above technical solution, the refrigeration cycle mechanism includes a cooler, a refrigeration chamber, a circulating fan, a temperature sensor, and a temperature control switch. The storage box is provided with the refrigeration chamber, and the cooler is fixedly connected to the storage box corresponding to the refrigeration chamber. The refrigeration chamber is connected to the semen storage area through a first circulation pipe, and the refrigeration chamber is connected to the semen dispensing area through a second circulation pipe. A mounting platform is fixedly connected to the refrigeration chamber corresponding to the first circulation pipe or the second circulation pipe, and the circulating fan is fixedly connected to the mounting platform. The temperature sensor is fixedly connected in the storage area, and both the cooler and the temperature sensor are electrically connected to the temperature control switch.

[0013] In the above technical solution, a display screen is fitted onto the cover, and the display screen is electrically connected to the temperature sensor.

[0014] In the above technical solution, a power switch is fixedly connected to the storage box, the storage box is provided with a vehicle DC power interface and a three-phase AC interface, a controller is fixedly connected inside the storage box, and the power switch, cooler, circulating fan, vehicle DC power interface, three-phase AC interface, temperature control switch, and pump body are all electrically connected to the controller.

[0015] The beneficial effects of this utility model are: 1. The device integrates the storage of diluted semen with subsequent dispensing in the same storage box, realizing the entire process from batch storage to dispensing into usable doses. It is easy to carry and avoids the risk of temperature fluctuations and contamination caused by transferring semen during the dispensing process, greatly improving the continuity and safety of the operation. 2. The air inside the cooling chamber can be cooled by the refrigeration unit, and then the cold air is sent into the storage area by the circulating fan. The low temperature achieves low-temperature preservation of semen, and the storage area and the cooling chamber form a closed airflow, reducing external temperature interference and thus greatly reducing energy consumption. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the structure of the cover when it is opened in this utility model; Figure 2 This is a schematic diagram of the cover of this utility model from another angle when it is opened; Figure 3 This is a schematic diagram of the structure of the cover when it is closed in this utility model; Figure 4 This is a schematic diagram of the cover of this utility model from another angle when it is closed; Figure 5 This is a schematic diagram of the internal structure of the storage box in this utility model; Figure 6 This is a schematic diagram of the connection structure between the first circulation pipe and the second circulation pipe in this utility model; Figure 7 This is a schematic diagram of the pump body connection structure in this utility model; Figure 8 This is a schematic diagram of the structure of the semen dilution preservation test tube in this utility model; Figure 9 This is a schematic diagram of the dispensing capillary tube in this utility model.

[0017] In the diagram: 100 Storage box, 101 Storage area, 1011 Semen storage area, 1012 Semen dispensing area, 102 Cover, 103 Sealing strip, 104 Lock, 105 Test tube rack, 106 Thin tube rack, 107 Thin tube storage chamber, 108 Sealing plate, 109 Partition, 1091 Air vent; 200 test tube for diluting semen, 201 test tube body, 202 test tube stopper cap, 203 test tube mouth, 204 small stopper for test tube; 300 thin tube for dispensing, 301 thin tube body, 302 thin tube stopper cap, 303 thin tube opening, 304 small thin tube stopper; 401 Pump body, 402 First conduit, 403 Negative pressure suction head, 404 Suction head plug, 405 Second conduit, 406 Injection head, 407 Injection plug; 501 Refrigerator, 502 Refrigeration chamber, 503 Circulating fan, 504 Temperature sensor, 505 Temperature control switch, 506 First circulation pipe, 507 Second circulation pipe, 508 Mounting platform; 600 display screen; 700 power switch; 800 vehicle-mounted DC power supply interface; 900 three communication interfaces. Detailed Implementation

[0018] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0019] Please see Figures 1-9 A portable, long-term low-temperature preservation device for diluted semen from sika deer includes a preservation box 100, a semen preservation test tube 200, and a dispensing tube 300. First, the preservation box 100 is composed of an inner and outer double shell, with the middle filled with high-efficiency heat-insulating materials such as polyurethane to minimize heat exchange between the interior and the external environment. It also has a base at the bottom. Inside the preservation box 100, there is a storage area 101 on one side. The storage area 101 is divided into a semen storage area 1011 and a semen dispensing area 1012 by a vertically fixed partition 109. At least one air vent 1091 is provided on the partition 109 to ensure that cold air can circulate between the two zones and maintain a uniform temperature. In addition, a cover 102 is connected to the storage area 101 on the storage box 100 via a hinge. The contact edge between the cover 102 and the storage box 100 is fitted with a sealing strip 103 and is tightly locked by a latch 104, thereby forming a basically sealed low-temperature space when closed. Secondly, in the semen storage area 1011, a test tube rack 105 is installed by means of screw fixing or slot embedding. The test tube rack 105 has multiple holes that match the shape of the diluted semen storage test tube 200, which can stably hold multiple sets of test tubes. The diluted semen preservation test tube 200 specifically includes a test tube body 201 and a test tube stopper cap 202 for sealing the opening of the test tube body 201. The test tube stopper cap 202 is usually made of elastic materials such as rubber or silicone to provide good sealing. In particular, the test tube stopper cap 202 has a through-hole test tube opening 203 with a small diameter in the center. When not in use, the test tube opening 203 is tightly blocked by an independent small test tube stopper 204 (also made of elastic material). In the semen dispensing area 1012, a capillary rack 106 is installed in the same manner to neatly arrange and fix multiple dispensing capillary tubes 300. The structure of the dispensing capillary tube 300 is similar to that of the storage test tube, but smaller in size. It includes a capillary tube body 301 and a capillary tube stopper 302 for blocking the opening of the capillary tube body 301. The capillary tube stopper 302 is also provided with a capillary tube opening 303, which is sealed by a small capillary tube stopper 304 when not in use. To achieve seamless transfer of semen from the storage tube to the dispensing capillary tube 300, a miniature peristaltic pump or diaphragm pump is fixedly installed inside the storage box 100 as a pump body 401. The pump body 401 has an inlet and an outlet. The inlet of the pump body 401 is connected to a negative pressure suction head 403 through a flexible first conduit 402. The negative pressure suction head 403 is made of hard plastic or stainless steel and its outer diameter is slightly smaller than the diameter of the test tube opening 203 to facilitate insertion. In addition, a suction head plug 404 made of elastic material is fixedly sleeved on the tube wall of the negative pressure suction head 403. When the negative pressure suction head 403 is inserted into the test tube body 201 through the test tube opening 203, the suction head plug 404 blocks the test tube opening 203, thereby effectively sealing the test tube opening 203 during the process of aspirating semen, preventing air from entering, and ensuring that the pump body 401 can generate sufficient negative pressure. The outlet of the pump body 401 is connected to the injection head 406 through another flexible second conduit 405. The outer diameter of the injection head 406 is slightly smaller than the diameter of the capillary opening 303. Similarly, an injection plug 407 made of elastic material is fixedly sleeved on the tube wall of the injection head 406. When the injection head 406 is inserted into the capillary opening 303, the injection plug 407 will block the capillary opening 303, so that the semen discharged by the pump body 401 can be completely injected into the capillary body 301 without leakage. Furthermore, the storage box 100 is also equipped with a refrigeration circulation mechanism to improve the low-temperature storage of semen. Specifically, the refrigeration circulation mechanism includes a cooler 501, a refrigeration chamber 502, a circulation fan 503, a temperature sensor 504, and a temperature control switch 505. That is, there is an independent refrigeration chamber 502 in the storage box 100. The cold end of the cooler 501 (preferably a semiconductor cooler 501) is embedded in the refrigeration chamber 502, while the hot end is located outside. The cooling fan that works with it can dissipate heat to the outside of the box. Furthermore, the cooling chamber 502 is connected to the semen storage area 1011 through the first circulation pipe 506 and to the semen dispensing area 1012 through the second circulation pipe 507. A mounting platform 508 is fixed inside the cooling chamber 502, on which a circulation fan 503 is installed. When the circulation fan 503 is working, it drives the cold air in the cooling chamber 502 to enter the semen storage area 1011 through the first circulation pipe 506, then enter the semen dispensing area 1012 through the air vent 1091 on the partition 109, and finally return to the cooling chamber 502 through the second circulation pipe 507 to be cooled again, thus forming a closed and continuous airflow circulation loop. Within the storage area 101, preferably near the test tube rack 105 in the semen storage area 1011, a temperature sensor 504 (such as a PT100 platinum resistance thermometer or a digital temperature chip) is fixedly installed. The temperature sensor 504, the cooler 501, and the circulating fan 503 are all electrically connected to a temperature control switch 505. The temperature sensor 504 monitors the temperature of the storage area 101 in real time, and the temperature control switch 505 is used to set the temperature. Finally, to facilitate the storage and retrieval of spare empty tubes, an independent tube storage chamber 107 is provided on the other side of the storage box 100. It is connected to the outside through a retrieval port. A sealing plate 108 is slidably connected to the storage box 100 through a slide rail mechanism. Users can open or close the retrieval port by pushing or pulling the sealing plate 108. In addition, a display screen 600 (such as an LCD or OLED screen) is fitted and installed on the cover 102. The display screen 600 is connected to the temperature sensor 504 via wires to display the current temperature value of the storage area 101 in real time and intuitively, so as to facilitate user monitoring. In terms of power supply, a power switch 700 is installed on the outer wall of the storage box 100. The power switch 700 includes a main power switch 700, a cooling power switch 700, and a sub-power switch 700. In order to adapt to various usage scenarios, a vehicle DC power interface 800 and a three-phase AC interface 900 are set on the box. A controller (such as a microprocessor-based control board) is also fixedly connected inside the storage box 100. The power switch 700, the cooler 501, the circulating fan 503, the pump 401, the vehicle DC power interface 800, the three-phase AC interface 900, the temperature control switch 505, etc. are all electrically connected to the controller. The controller is responsible for the power management, mode switching, and coordination of the orderly operation of various components of the whole machine.

[0020] The specific working method of this utility model is as follows: First, the collected and diluted sika deer semen is dispensed into multiple diluted semen storage test tubes 200 and placed on the test tube rack 105 in the semen storage area 1011. Empty dispensing tubes 300 are placed on the tube rack 106 in the semen dispensing area 1012. The cap 102 is closed and locked. The main power switch 700 and the cooling power switch 700 are turned on, and the cooling circulation mechanism starts working, rapidly cooling the storage area 101 and maintaining it at the preset low-temperature storage temperature. Specifically, when the device is powered on, the controller first sends instructions to the cooler 501 (semiconductor cooling chip) and the circulating fan 503 according to the preset program. The cooler 501 starts working, and its cold end face rapidly absorbs the heat of the air in the cooling chamber 502, causing the air temperature in the chamber to drop sharply. At the same time, the circulating fan... The circulating fan 503 starts synchronously, forcibly blowing the low-temperature air generated in the cooling chamber 502 into the semen storage area 1011 through the first circulation pipe 506, thereby cooling the storage area 101. Under the suction of the circulating fan 503, the air is drawn back into the cooling chamber 502 through the second circulation pipe 507. At this time, the cold end face of the cooler 501 cools the returning air again, completing a complete thermal cycle. In addition, the temperature sensor 504 continuously monitors the actual temperature of the storage area 101 and feeds the real-time data back to the controller. The controller compares the monitored temperature value with the target temperature value set by the user (set by the temperature control switch 505). When the measured temperature is higher than the upper limit of the set value, the controller will command the cooler 501 and the circulating fan 503 to run at full capacity for forced cooling. When the temperature drops to the set range, the controller can adjust the power of the cooler 501 (e.g., a PWM-controlled cooling chip) or switch to an intermittent working mode to match the cooling capacity with the ambient heat load, thereby achieving dynamic temperature balance and precise stability. Secondly, when it is necessary to perform pre-insemination repackaging, the operator opens the box lid. At this time, all semen samples and repackaging tubes 300 are still under low-temperature protection. The operator removes the small stopper 204 on the diluted semen preservation test tube 200, exposing the test tube opening 203. The negative pressure pipette 403 is inserted through the test tube opening 203 below the liquid surface of the test tube body 201. The pipette stopper 404 blocks the test tube opening 203. At the same time, the small stopper 304 on the repackaging tube 300 is removed, exposing the tube opening 303. The filling head 406 is inserted into the tube body 301. Similarly, the filling stopper 407 on it will seal the tube opening 303. The operator activates the pump body 401 by operating the dispensing power switch 700. The pump body 401 starts working and generates a precisely controlled negative pressure inside. The negative pressure is transmitted to the negative pressure suction head 403 through the first conduit 402. Under the action of negative pressure, the semen in the test tube is steadily drawn out, flows through the first conduit 402, the inside of the pump body 401, and the second conduit 405, and is finally discharged from the filling head 406. The discharged semen is directly injected into the dispensing capillary tube 300. The running time or revolutions of the pump body 401 can be preset to achieve quantitative dispensing and ensure that the semen dosage in each dispensing capillary tube 300 is consistent. When the preset dispensing volume is completed, the pump body 401 will stop automatically. The operator will first pull the dispensing head 406 out of the already filled dispensing capillary tube 300. The moment the dispensing plug 407 leaves the capillary tube opening 303, the small plug 304 should be immediately pushed back in to seal the capillary tube. Then the dispensing head 406 can be used with another dispensing capillary tube 300 in the same way.

[0021] It will be apparent to those skilled in the art that this invention is not limited to the details of the exemplary embodiments described above, and that it can be implemented in other specific forms without departing from the spirit or essential characteristics of this invention. Therefore, the embodiments should be considered illustrative and non-limiting in all respects, and the scope of this invention is defined by the appended claims rather than the foregoing description. Thus, it is intended that all variations falling within the meaning and scope of equivalents of the claims be included within this invention. No reference numerals in the claims should be construed as limiting the scope of the claims.

[0022] Furthermore, it should be understood that although this specification describes embodiments, not every embodiment contains only one independent technical solution. This narrative style is merely for clarity. Those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A portable, low-temperature, long-term preservation device for diluted sika deer semen, comprising a preservation box (100), a semen preservation test tube (200), and dispensing capillary tubes (300), characterized in that: The storage box (100) has a storage area (101) on one side. A test tube rack (105) and a thin tube rack (106) are fixedly connected in the storage area (101). Multiple sets of diluted semen storage test tubes (200) are placed on the test tube rack (105), and multiple sets of dispensing thin tubes (300) are placed on the thin tube rack (106). A cover (102) is rotatably connected to the storage box (100) corresponding to the storage area (101). The cover (102) is connected to the storage box (100) by a latch (104). A pump body (401) is fixedly connected inside the storage box (100). A negative pressure suction head (403) is fixedly connected to the inlet of the pump body (401) through a first conduit (402). The negative pressure suction head (403) is matched with the diluted semen storage test tube (200). A filling head (406) is fixedly connected to the outlet of the pump body (401) through a second conduit (405). The filling head (406) is matched with the dispensing capillary tube (300). The storage box (100) is equipped with a refrigeration circulation mechanism, which is connected to the pipeline of the storage area (101).

2. The portable low-temperature long-term preservation device for diluted sika deer semen according to claim 1, characterized in that: The storage box (100) is also provided with a thin tube storage cavity (107). The storage box (100) is provided with a take-out port that communicates with the thin tube storage cavity (107). A sealing plate (108) is slidably connected to the storage box (100), and the sealing plate (108) closes the take-out port.

3. The portable low-temperature long-term preservation device for diluted sika deer semen according to claim 1, characterized in that: The diluted semen preservation test tube (200) includes a test tube body (201) and a test tube stopper cap (202) that blocks the opening of the test tube body (201). The test tube stopper cap (202) is provided with a test tube opening (203), and the test tube opening (203) is blocked by a small test tube stopper (204).

4. The portable low-temperature long-term preservation device for diluted sika deer semen according to claim 3, characterized in that: The dispensing cap (300) includes a cap body (301) and a cap plug (302) that plugs the opening of the cap body (301). The cap plug (302) has a cap opening (303) and the cap opening (303) is plugged with a small cap plug (304).

5. A portable, low-temperature, long-term preservation device for diluted sika deer semen according to claim 3, characterized in that: A pipette plug (404) is fixedly connected to the negative pressure pipette (403). When the negative pressure pipette (403) is inserted into the test tube body (201) through the test tube opening (203), the pipette plug (404) blocks the test tube opening (203).

6. A portable, low-temperature, long-term preservation device for diluted sika deer semen according to claim 4, characterized in that: An injection plug (407) is fixedly connected to the injection head (406). When the injection head (406) is inserted into the thin tube body (301) through the thin tube opening (303), the injection plug (407) blocks the thin tube opening (303).

7. The portable low-temperature long-term preservation device for diluted sika deer semen according to claim 1, characterized in that: A partition (109) is fixedly connected in the storage area (101). One side of the partition (109) is the semen storage area (1011), and the other side is the semen dispensing area (1012). The test tube rack (105) is fixedly connected in the semen storage area (1011), and the thin tube rack (106) is fixedly connected in the semen dispensing area (1012). An air vent (1091) is provided on the partition (109).

8. A portable, low-temperature, long-term preservation device for diluted sika deer semen according to claim 7, characterized in that: The refrigeration cycle mechanism includes a cooler (501), a refrigeration chamber (502), a circulating fan (503), a temperature sensor (504), and a temperature control switch (505). The storage box (100) is provided with the refrigeration chamber (502). The cooler (501) is fixedly connected to the storage box (100) corresponding to the refrigeration chamber (502). The refrigeration chamber (502) is connected to the semen storage area (1011) through a first circulation pipe (506). The refrigeration chamber (502) is connected to the semen dispensing area (1012) through a second circulation pipe (507). An installation platform (508) is fixedly connected to the refrigeration chamber (502) corresponding to the first circulation pipe (506) or the second circulation pipe (507). The circulating fan (503) is fixedly connected to the installation platform (508). The temperature sensor (504) is fixedly connected in the storage area (101), and both the cooler (501) and the temperature sensor (504) are electrically connected to the temperature control switch (505).

9. A portable, low-temperature, long-term preservation device for diluted sika deer semen according to claim 8, characterized in that: A display screen (600) is fitted onto the cover (102), and the display screen (600) is electrically connected to the temperature sensor (504).

10. A portable, low-temperature, long-term preservation device for diluted sika deer semen according to claim 8, characterized in that: A power switch (700) is fixedly connected to the storage box (100). The storage box (100) is provided with a vehicle DC power interface (800) and a three-phase AC interface (900). A controller is fixedly connected inside the storage box (100). The power switch (700), the cooler (501), the circulating fan (503), the vehicle DC power interface (800), the three-phase AC interface (900), the temperature control switch (505), and the pump body (401) are all electrically connected to the controller.

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