A novel chromatographic separation device for protein purification

Abstract

The utility model relates to a novel chromatography separation device for protein purification belongs to protein purification separation technical field, this novel chromatography separation device for protein purification, include: separation equipment and filter mechanism, wherein one connector is connected with the material taking structure through the connecting pipe, another connector is connected with the feed inlet through the mounting head, the inside of filter box is provided with the filter membrane for carrying out the filtration to sample, the upper portion of filter box is provided with the flip cover convenient for the worker to replace filter membrane, the above -mentioned chromatography separation device, is provided with the filter mechanism through setting up outside the feed inlet, carries out the isolation filtration to the float in the biological sample that enters the feed inlet inside through centrifugation through the two groups of filter membranes that set up in the filter box, reduces the float in biological sample and follows the sample to enter the host inside and blocks the chromatography column internal medium, and then needs frequently to clean chromatography column replacement.

Description

Technical Field

[0001] This utility model relates to the field of protein purification and separation technology, and in particular to a novel chromatographic separation device for protein purification. Background Technology

[0002] In the fields of biopharmaceutical, enzyme engineering, and proteomics research, protein purification is a core step in obtaining high-purity, high-activity target proteins. Chromatography, due to its advantages of high separation efficiency and strong specificity, has become the mainstream method for protein purification. Protein separation and purification instruments are the main equipment in this field.

[0003] In actual chromatographic separation operations, since natural biological samples (such as cell lysates, fermentation broths, and tissue extracts) generally contain complex impurities, the biological samples are centrifuged before chromatographic separation. The centrifuge causes the precipitates, cell debris, and large particulate impurities in the sample to settle to the bottom of the tube, and the clear liquid in the upper layer is taken for subsequent processing. Because traditional centrifuges can only remove large-particle precipitates and cell debris from samples, they are unable to remove small suspended particles. As a result, a small amount of impurities remain in the clear liquid above the centrifuged sample. When the sample is then fed into a protein separation and purification instrument, the impurities enter the chromatography column and clog the pores. Workers need to frequently replace or clean the chromatography column, making the operation quite cumbersome. Utility Model Content

[0004] Therefore, it is necessary to provide a novel chromatographic separation device for protein purification, which addresses the problem that a small amount of impurities still exist in the supernatant after centrifugation. These impurities can then clog the pores of the chromatography column in a protein separation and purification instrument, requiring frequent column replacement or cleaning by workers, which is quite cumbersome.

[0005] A novel chromatographic separation device for protein purification includes: a separation device comprising a main unit, a material handling structure, a material dispensing tray, and a material inlet, wherein the material handling structure is disposed on the side of the main unit, the material dispensing tray is disposed below the material handling structure, and the material inlet is disposed on the side wall of the main unit. The filtration mechanism includes a filter box, with connectors on both sides of the filter box. One connector is connected to the material handling structure via a connecting pipe, and the other connector is connected to the feed inlet via an installation head. The filter box contains a filter membrane for filtering samples, and a flip-top cover is provided on top of the filter box for easy replacement of the filter membrane by workers.

[0006] In one embodiment, the filter box has two sets of placement slots inside, the filter membrane is placed inside the placement slots, one end of the flip cover is rotatably connected to the filter box, and the other end of the flip cover is connected to the outer arc surface of the filter box by bolts.

[0007] In one embodiment, sealing rings are provided on both side walls of the flip cover, and a groove corresponding to the sealing ring is formed on the inner wall of the filter box, with the sealing ring fully disposed inside the groove.

[0008] In one embodiment, the end of the connecting pipe connected to the connector is provided with a fixing head, the fixing head is provided with a plug ring inside, the end side wall of the connector is provided with a slot, the plug ring is inserted into the inside of the slot, and the outer arc surface of the plug ring is threadedly connected to the threaded groove of the inner wall of the slot.

[0009] In one embodiment, the ends of the two sets of connectors are provided with rubber rings on the outside of the slot, and the rubber rings are in close contact with the inner wall of the fixing head.

[0010] In one embodiment, a sealing ring is provided on the outer side of the end of the feed inlet, one side of the mounting head is threadedly connected to the connector, and the other side of the mounting head is provided with a protrusion facing the side wall of the feed inlet. A groove is provided inside the protrusion, and the groove is fitted on the outer side of the sealing ring.

[0011] In one embodiment, a soft rubber ring is provided on the side wall of the protrusion located outside the feed port, and the soft rubber ring is fixed to the outside of the feed port by a cable tie.

[0012] In one embodiment, the pore size of the filter membrane on the side closer to the fixing head is larger than the pore size of the filter membrane on the other side.

[0013] Beneficial effects 1. The above-mentioned chromatography separation device has a filter mechanism set on the outside of the feed inlet. The two sets of filter membranes set inside the filter box isolate and filter the floating matter in the biological sample that has been centrifuged into the feed inlet. This reduces the floating matter in the biological sample from following the sample into the main unit and clogging the medium inside the chromatography column, thus reducing the need for frequent cleaning and replacement of the chromatography column. 2. The above-mentioned chromatography separation device has an installation head on the outside of the feed inlet, which can be connected to the connector on the side wall of the filter box. The operator can replace the filter membrane inside the filter box by lifting the cover. At the same time, sealing rings are provided on both sides of the cover to ensure the airtightness between the filter box and the inside of the cover. Attached Figure Description

[0014] To more clearly illustrate the technical solutions in this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of this utility model. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0015] Figure 1 This is a schematic diagram of the overall external structure of this utility model; Figure 2 This is a schematic diagram of the disassembled separation device and filtration mechanism of this utility model. Figure 3 This is a schematic diagram of the disassembled structure of the filter mechanism of this utility model; Figure 4 This is a cross-sectional structural diagram of the filter box and connector of this utility model; Figure 5 This utility model Figure 4 Enlarged view of point A in the middle; Figure 6 This is a schematic diagram of the flip-top opening state of this utility model; Figure 7 This is a cross-sectional view of the fixed head structure of this utility model.

[0016] Figure label: 100. Separation equipment; 101. Main unit; 102. Material handling structure; 103. Discharge tray; 104. Feed inlet; 105. Insert ring; 106. Connecting pipe; 107. Fixing head; 200. Filtration mechanism; 201. Filter box; 202. Flip cover; 203. Connecting head; 204. Slot; 205. Rubber ring; 206. Threaded groove; 207. Placement slot; 208. Filter membrane; 209. Sealing ring; 210. Groove; 211. Mounting head; 212. Protrusion sleeve; 213. Slot; 214. Soft rubber ring; 215. Sealing ring. Detailed Implementation

[0017] To make the objectives, technical solutions, and advantages of the embodiments of this utility model clearer, the technical solutions of the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of this utility model. All other embodiments obtained by those skilled in the art based on the embodiments of this utility model without creative effort are within the scope of protection of this utility model.

[0018] The following is combined Figures 1-7 This invention describes a novel chromatographic separation apparatus for protein purification.

[0019] In one embodiment, a novel chromatographic separation device for protein purification includes: a separation device 100, which consists of a main unit 101, a material receiving structure 102, a material dispensing tray 103, and a material inlet 104. The material receiving structure 102 is disposed on the side of the main unit 101, the material dispensing tray 103 is disposed below the material receiving structure 102, and the material inlet 104 is disposed on the side wall of the main unit 101. Additional notes: The model of the separation device 100 in this application may be: SDL-030; like Figure 2 , Figure 3 , Figure 4 and Figure 6 As shown, the filtration mechanism 200 includes a filter box 201. Connectors 203 are respectively provided on both sides of the filter box 201. One connector 203 is connected to the material receiving structure 102 through a connecting pipe 106, and the other connector 203 is connected to the feed inlet 104 through an mounting head 211. A filter membrane 208 for filtering samples is provided inside the filter box 201. A flip cover 202 is provided on the top of the filter box 201 to facilitate the replacement of the filter membrane 208 by workers. The surface pore size of the filter membrane 208 on the side closer to the fixing head 107 is larger than the surface pore size of the filter membrane 208 on the other side. The filter box 201 has two sets of placement slots 207 inside, and the filter membrane 208 is placed inside the placement slots 207. One end of the flip cover 202 is rotatably connected to the filter box 201, and the other end of the flip cover 202 is connected to the outer arc surface of the filter box 201 by bolts. Both side walls of the flip cover 202 are provided with sealing rings 209. The inner wall of the filter box 201 has a groove 210 corresponding to the sealing ring 209, and the sealing ring 209 is fully disposed inside the groove 210. A filter mechanism 200 is connected to the outside of the feed inlet 104 inside the separation device 100. The filter membrane 208 inside the filter box 201 inside the filter mechanism 200 filters the floating matter in the biological sample entering the feed inlet 104. After the filter membrane 208 has been filtering for a long time, the operator can lift the flip cover 202 and replace the filter membrane 208 inside the filter box 201 with tools. The operation of replacing the filter membrane 208 is simpler than the operation of replacing the chromatography column inside the separation device 100, thereby reducing the replacement frequency of the chromatography column. When the operator needs to replace the filter membrane 208, the flip cover 202 is lifted, the filter membrane 208 is replaced with a tool, and the new filter membrane 208 is placed inside the placement slot 207 inside the filter box 201. Then the flip cover 202 is closed on top of the filter box 201. After the flip cover 202 is closed, the sealing rings 209 provided on both sides of the flip cover 202 are engaged inside the groove 210. The sealing rings 209 are fully disposed inside the groove 210, and the sealing rings 209 are used to ensure the airtightness between the filter box 201 and the flip cover 202. like Figure 4 , Figure 5 and Figure 7 As shown, a fixing head 107 is provided at the end of the connecting pipe 106 that connects to the connector 203. A retaining ring 105 is provided inside the fixing head 107. A slot 204 is provided on the side wall of the end of the connector 203. The retaining ring 105 is inserted into the slot 204. The outer arc surface of the retaining ring 105 is threadedly connected to the threaded groove 206 on the inner wall of the slot 204. Rubber rings 205 are provided at the ends of the two sets of connectors 203 on the outside of the slot 204. The rubber rings 205 are in close contact with the inner wall of the fixing head 107. In this embodiment, a fixing head 107 is provided at the end of the connecting tube 106, and a retaining ring 105 is provided inside the fixing head 107. The retaining ring 105 is inserted into the slot 204 inside the connector 203, and the retaining ring 105 is threadedly connected to the threaded groove 206 opened on the inner wall of the slot 204. A rubber ring 205 is provided at the outer end of the slot 204. After the retaining ring 105 is threadedly connected to the connector 203, the rubber ring 205 ensures the sealing between the fixing head 107 and the connector 203, preventing the sample from flowing out from the gap during use and causing contamination to the workbench surface. like Figure 4 As shown, a sealing ring 215 is provided on the outer side of the end of the feed inlet 104. One side of the mounting head 211 is threadedly connected to the connector 203. The other side of the mounting head 211 is provided with a protrusion 212 facing the side wall of the feed inlet 104. A groove 213 is provided inside the protrusion 212. The groove 213 is fitted on the outer side of the sealing ring 215. A soft rubber ring 214 is provided on the side wall of the protrusion 212 outside the feed port 104. The soft rubber ring 214 is fixed to the outside of the feed port 104 by a cable tie. In this embodiment, a sealing ring 215 is provided on the end side wall of the feed inlet 104, and an installation head 211 is sleeved on the outside of the sealing ring 215. The sealing ring 215 and the inner wall of the groove 213 inside the installation head 211 are filled together, and the sealing ring 215 is used to ensure the sealing of the connection between the installation head 211 and the feed inlet 104. Furthermore, a protrusion 212 is provided on the side wall of the mounting head 211 facing the feed inlet 104. The protrusion 212 is set at the outer end of the feed inlet 104 by a cable tie, so that the mounting head 211 can be installed according to the diameter of different feed inlets 104, and the filter box 201 cannot be installed due to the fixed mounting head 211 setting.

[0020] Working principle: Before use, the operator first puts the protrusion 212 on the side wall of the mounting head 211 onto the outer end of the feed inlet 104, and uses cable ties to fix the protrusion 212 to the outside of the feed inlet 104. Then, the connectors 203 on both sides of the filter box 201 are connected to the mounting head 211 and the fixing head 107 respectively. After the connection is completed, the connecting pipe 106 is placed inside the pipe clamp on the side wall of the main unit 101, and the pipe clamp is used to support the connecting pipe 106. After installation, the centrifuged biological sample solution placed inside the feeding tray 103 is drawn by the feeding structure 102 and transported to the filter box 201. The biological sample solution is filtered by the filter box 201 before entering the feed port 104, thereby reducing the amount of floating matter in the sample solution entering the chromatography column inside the host 101, and thus reducing the number of times the operator needs to clean and replace the chromatography column.

[0021] The above embodiments are only used to illustrate the technical solutions of this utility model, and are not intended to limit it. Although this utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions will not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of this utility model.

Claims

1. A novel chromatographic separation device for protein purification, characterized in that, include: The separation device (100) consists of a main unit (101), a material handling structure (102), a material discharging tray (103), and a material inlet (104). The material handling structure (102) is located on the side of the main unit (101), the material discharging tray (103) is located below the material handling structure (102), and the material inlet (104) is located on the side wall of the main unit (101). The filtration mechanism (200) includes a filter box (201). Connectors (203) are provided on both sides of the filter box (201). One connector (203) is connected to the material receiving structure (102) through a connecting pipe (106), and the other connector (203) is connected to the feed inlet (104) through an installation head (211). The filter box (201) is provided with a filter membrane (208) for filtering the sample. A flip cover (202) is provided on the top of the filter box (201) to facilitate the replacement of the filter membrane (208) by the worker.

2. The novel chromatographic separation device for protein purification according to claim 1, characterized in that, The filter box (201) has two sets of placement slots (207) inside. The filter membrane (208) is placed inside the placement slots (207). One end of the flip cover (202) is rotatably connected to the filter box (201), and the other end of the flip cover (202) is connected to the outer arc surface of the filter box (201) by bolts.

3. A novel chromatographic separation device for protein purification according to claim 2, characterized in that, Both sides of the flip cover (202) are provided with sealing rings (209), and the inner wall of the filter box (201) is provided with a groove (210) corresponding to the sealing ring (209), and the sealing ring (209) is fully disposed inside the groove (210).

4. A novel chromatographic separation device for protein purification according to claim 1, characterized in that, The end of the connecting pipe (106) connected to the connector (203) is provided with a fixing head (107), and the fixing head (107) is provided with a plug ring (105). The end side wall of the connector (203) is provided with a slot (204). The plug ring (105) is inserted into the slot (204), and the outer arc surface of the plug ring (105) is threadedly connected to the threaded groove (206) of the inner wall of the slot (204).

5. A novel chromatographic separation device for protein purification according to claim 4, characterized in that, The ends of the two sets of connectors (203) are provided with rubber rings (205) on the outside of the slot (204), and the rubber rings (205) are in close contact with the inner wall of the fixing head (107).

6. A novel chromatographic separation device for protein purification according to claim 5, characterized in that, A sealing ring (215) is provided on the outer side of the end of the feed inlet (104). One side of the mounting head (211) is threadedly connected to the connector (203). A protruding sleeve (212) is provided on the other side of the mounting head (211) facing the side wall of the feed inlet (104). A slot (213) is provided inside the protruding sleeve (212). The slot (213) is fitted on the outer side of the sealing ring (215).

7. A novel chromatographic separation device for protein purification according to claim 6, characterized in that, The protrusion (212) is provided with a soft rubber ring (214) on the side wall outside the feed port (104), and the soft rubber ring (214) is fixed to the outside of the feed port (104) by a cable tie.

8. A novel chromatographic separation device for protein purification according to claim 1, characterized in that, The pore size of the filter membrane (208) on the side closer to the fixing head (107) is larger than the pore size of the filter membrane (208) on the other side.

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