A disposable filtration centrifugation device

Abstract

The utility model discloses a kind of disposable filtration centrifugal separation devices, it is characterized in that, including front cabin, filter section and rear cabin, the first end of the front cabin and the filter section is threadedly connected, the second end of the rear cabin and the filter section is threadedly connected;It further includes lid, the lid and the front cabin are threadedly connected;The first end of the filter section is equipped with first filter screen, the second end of the filter section is equipped with second filter screen, the first filter screen and the second filter screen are arranged in parallel;A kind of disposable filtration centrifugal separation device front cabin and lid combination use, for the temporary storage of the mixture digestion of storage liquid and sample;Front cabin, filter section and rear cabin combination use, for centrifugal filtration.A kind of disposable filtration centrifugal separation device is realized by modularization combination " mixing-filtering-centrifugation " whole process, reduce sample transfer frequency, reduce pollution risk, meet the demand of new experiment process.

Description

Technical Field

[0001] This utility model belongs to the field of medical consumables technology, specifically relating to a disposable filter centrifugal separation device. Background Technology

[0002] In traditional bronchoalveolar lavage fluid (BALF) slide preparation methods, filtration is typically not performed during sample processing. However, new experimental requirements necessitate the addition of filtration as a crucial step. The primary function of filtration is to break down the mucus, allowing subsequent digestion to proceed more smoothly and efficiently. Without mucus fragmentation, large mucus particles remain isolated, leading to cell clumping after centrifugation. This cell clumping severely impacts the quality of subsequent slide preparation, thus interfering with observational results.

[0003] Currently, the consumables used in the bronchoalveolar lavage fluid preparation process are all general-purpose types, such as test tubes, ordinary centrifuge tubes, and filters. These consumables are used individually and cannot meet new experimental needs. The same consumable cannot simultaneously perform the functions of sample storage and sample centrifugation. Utility Model Content

[0004] To achieve the above objectives, the present invention adopts the following technical solution:

[0005] A disposable filtration centrifugal separation device includes a front chamber, a filter section, and a rear chamber. The front chamber is threaded to a first end of the filter section, and the rear chamber is threaded to a second end of the filter section. It also includes a cover, which is threaded to the front chamber.

[0006] The front compartment is a cylindrical cavity; the filter section is a cylindrical cavity, with a first filter screen at the first end and a second filter screen at the second end, the first filter screen and the second filter screen being arranged in parallel; the upper part of the rear compartment is cylindrical and the lower part is a conical cavity.

[0007] As a further embodiment of this utility model: the first filter screen and the first end of the filter section are smoothly transitioned by an arc-shaped surface; the second filter screen and the second end of the filter section are smoothly transitioned by an arc-shaped surface.

[0008] As a further embodiment of this utility model, the filter section is integrally formed with the first filter screen and the second filter screen.

[0009] As a further aspect of this invention, the area of ​​the first filter screen is smaller than the area of ​​the second filter screen.

[0010] As a further embodiment of this utility model: the end of the front compartment is provided with a first internal thread, the first end of the filter section is provided with a first external thread, the second end of the filter section is provided with a second external thread, the first external thread and the second external thread have different sizes, the end of the rear compartment is provided with a second internal thread, and the end of the cover is provided with a third external thread; the first internal thread is matched with the first external thread and the third external thread respectively, and the second internal thread and the second external thread are matched.

[0011] As a further embodiment of this invention, a groove is provided at the other end of the cover.

[0012] As a further embodiment of this utility model: the front chamber, the filter section, the rear chamber, and the cover are each provided with an elongated protrusion.

[0013] As a further embodiment of this utility model: the front chamber, the filter section, and the rear chamber cover are all transparent.

[0014] The beneficial effects of this utility model are:

[0015] A disposable filtration centrifuge device consists of four parts: a front chamber, a filter section, a rear chamber, and a lid. The front chamber and lid are used together for mixing, digesting, and temporarily storing the preservation solution and sample. The front chamber, filter section, and rear chamber are used together for centrifugal filtration. The filter section has two layers of filter screens. During centrifugation, all formed elements, including cells, can pass smoothly through the two layers of filter screens and enter the rear chamber for enrichment. Simultaneously, these two layers of filter screens effectively isolate mucus, preventing it from flowing into the rear chamber and causing cell clumping. This disposable filtration centrifuge device achieves a complete "mixing-filtration-centrifugation" process through modular combination, reducing the number of sample transfers, lowering the risk of contamination, and meeting the needs of new experimental procedures. Attached Figure Description

[0016] Figure 1 This is a partial structural diagram of a disposable filtration and centrifugal separation device;

[0017] Figure 2 This is a schematic diagram of another part of a disposable filtration centrifugal separation device;

[0018] Figure 3 This is a schematic diagram of the front chamber of a disposable filtration centrifugal separation device.

[0019] Figure 4 This is a schematic diagram of the filter section of a disposable centrifugal filtration device.

[0020] Figure 5 This is a schematic diagram of the filter section of a disposable centrifugal filtration device.

[0021] Figure 6 This is a schematic diagram of the rear chamber of a disposable filtration centrifugal separation device.

[0022] Figure 7 This is a schematic diagram of the structure of the lid of a disposable filter centrifugal separator.

[0023] Figure 8 This is a schematic diagram of the structure of the lid of a disposable filter centrifugal separator.

[0024] As shown in the figure: 1-front chamber, 2-filter section, 21-first filter screen, 22-second filter screen, 23-arc surface, 3-rear chamber, 4-cover, 41-groove, 5-long strip protrusion. Detailed Implementation

[0025] The technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments. It should be understood that this application is not limited to the exemplary embodiments disclosed herein. All other embodiments obtained by those skilled in the art based on the embodiments of this application without creative effort are within the scope of protection of this application.

[0026] In the description of this utility model, it should be understood that the terms "length", "width", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

[0027] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this utility model, "a plurality of" means two or more, unless otherwise explicitly specified.

[0028] In the embodiments of this utility model, unless otherwise explicitly specified and limited, the terms "installation," "connection," "joining," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. For those skilled in the art, the specific meaning of the above terms in this utility model can be understood according to the specific circumstances.

[0029] Example 1

[0030] like Figure 1-8 As shown, a disposable filtration centrifugal separation device includes a front chamber 1, a filter section 2, and a rear chamber 3. The front chamber 1 and the first end of the filter section 2 are threadedly connected, and the rear chamber 3 and the second end of the filter section 2 are threadedly connected. It also includes a cover 4, which is threadedly connected to the front chamber 1.

[0031] The front compartment 1 is a cylindrical cavity; the filter section 2 is a cylindrical cavity, with a first filter screen 21 at the first end and a second filter screen 22 at the second end, and the first filter screen 21 and the second filter screen 22 are arranged in parallel; the upper part of the rear compartment 3 is cylindrical and the lower part is a conical cavity.

[0032] A disposable filtration centrifuge device consists of four parts: a front chamber 1, a filter section 2, a rear chamber 3, and a lid 4. The front chamber 1 and lid 4 are used together for mixing and temporarily storing the preservation solution and sample. The front chamber 1, filter section 2, and rear chamber 3 are used together for centrifugal filtration. The filter section 2 has two layers of filter screens. During centrifugation, all formed elements, including cells, can pass smoothly through the two layers of filter screens and enter the rear chamber 3 for enrichment. Simultaneously, these two layers of filter screens effectively isolate mucus, preventing it from flowing into the rear chamber 3 and causing cell clumping. This disposable filtration centrifuge device achieves a complete "mixing-filtration-centrifugation" process through modular combination, reducing the number of sample transfers, lowering the risk of contamination, and meeting the needs of new experimental procedures.

[0033] Specifically, first, combine the front chamber 1 and the lid 4. Add the preservation solution and sample to the front chamber 1, then screw on the lid 4. The chamber can be placed on a shaker at a suitable speed to mix thoroughly, promoting full contact between the preservation solution and the sample; or it can be left to stand for a period of time to allow the sample to digest naturally in the preservation solution. During centrifugation, unscrew the lid 4 and combine the front chamber 1, filter section 2, and rear chamber 3. Place the chamber in a centrifuge. Large particles, such as undigested mucus and tissue fragments, are trapped in the front chamber 1. The target liquid and cell pellet pass through the microporous structure of the filter section 2 and accumulate at the bottom of the rear chamber 3. After centrifugation, discard the front chamber 1 and filter section 2, retaining only the rear chamber 3. Observe the liquid stratification in the rear chamber 3. Slowly tilt the rear chamber 3 and carefully aspirate the supernatant with a pipette, retaining the bottom cell pellet. After resuspending with an appropriate amount of buffer, it can be used for smear preparation or subsequent detection.

[0034] Among them, such as Figure 3-8 As shown, the front chamber 1, filter section 2, rear chamber 3, and cover 4 are all connected by threads, facilitating easy assembly and disassembly. The front chamber 1 is a cylindrical cavity, convenient for sample collection and placement on the tube rack. The lower part of the rear chamber 3 is a conical cavity. After centrifugation and filtration, the formed elements in the sample will accumulate at the bottom of this conical cavity, facilitating subsequent removal of the supernatant. The rear chamber 3 may also be equipped with graduation markings for accurate sampling.

[0035] In this embodiment, as Figure 4 and 5 As shown, the first filter screen 21 and the first end of the filter section 2 are smoothly transitioned through an arc-shaped surface 23; the second filter screen 22 and the second end of the filter section 2 are also smoothly transitioned through an arc-shaped surface 23. The filter section 2 is integrally formed with the first filter screen 21 and the second filter screen 22.

[0036] Specifically, a first filter screen 21 and a second filter screen 22 are set at both ends of the filter section 2, and are smoothly transitioned through the arc-shaped surface 23, so that the liquid can flow smoothly down during the filtration process.

[0037] In this embodiment, as Figure 4 and 5 As shown, the area of ​​the first filter screen 21 is smaller than the area of ​​the second filter screen 22. The end of the front compartment 1 is provided with a first internal thread, the first end of the filter section 2 is provided with a first external thread, the second end of the filter section 2 is provided with a second external thread, and the dimensions of the first external thread and the second external thread are different. The end of the rear compartment 3 is provided with a second internal thread, and the end of the cover 4 is provided with a third external thread. The first internal thread matches the first external thread and the third external thread respectively, and the second internal thread matches the second external thread.

[0038] Specifically, the filtration direction is from the front chamber 1, passing through the first filter screen 21, then the second filter screen 22, and finally flowing into the rear chamber 3. When the liquid passes through the double-layer filter screen, the larger filtration area of ​​the lower layer disperses the fluid. As impurities gradually accumulate on the first filter screen 21, the filtration resistance increases, potentially leading to a decrease in fluid flow. The larger filtration area of ​​the second filter screen 22 can accommodate more impurities without significantly increasing resistance, thus maintaining a relatively stable fluid flow and improving overall filtration efficiency. This more effectively removes small particles, suspended solids, and other impurities from the fluid, improving the purity of the filtered material.

[0039] In this embodiment, as Figure 7 and 8 As shown, the other end of the cover 4 has a groove 41.

[0040] Specifically, the cover 4 matches the front cavity. The cover 4 has an external thread design and a uniform wall thickness. A groove 41 is provided at the end opposite to the external thread of the cover 4. In addition, due to the design of the groove 41, it is more convenient to insert a finger into the groove 41 and pick up the cover 4.

[0041] In this embodiment, as Figure 1-8 As shown, the front compartment 1, the filter section 2, the rear compartment 3, and the cover 4 are each provided with a long strip protrusion 5.

[0042] Specifically, the front chamber 1, filter section 2, rear chamber 3 and cover 4 are connected by threads, and long strip protrusions 5 are provided on the outer periphery as an anti-slip structure to facilitate the operator's rotation operation.

[0043] In this embodiment, the front compartment 1, the filter section 2, and the rear compartment 3 cover 4 are all transparent.

[0044] Specifically, the device is made of transparent material, making it easy to observe the sample's condition with the naked eye.

[0045] In summary, a disposable filtration centrifuge device, with its ingenious modular design, successfully realizes a complete and coherent experimental process of "mixing-filtration-centrifugation." In traditional experimental operations, samples need to undergo multiple transfers: from the mixing container to the filtration device, and then from the filtration device to the centrifuge. Each transfer inevitably increases the chance of the sample coming into contact with the external environment, thus greatly increasing the risk of sample contamination. This new disposable filtration centrifuge device integrates the three key steps of mixing, filtration, and centrifugation into one unit, significantly reducing the number of sample transfers. In this device, the sample can first be thoroughly mixed in the front chamber 1, then directly enter the filtration section 2 for efficient filtration, and finally undergo centrifugation in the rear chamber 3. The entire process eliminates the need for repeated movement of the sample between different containers. This not only significantly reduces the possibility of contamination due to sample transfer, ensuring the accuracy and reliability of experimental results, but also greatly improves experimental efficiency, saving researchers' time and effort.

[0046] It should also be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Without further limitations, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes said element.

[0047] The above description of the disclosed embodiments enables those skilled in the art to make or use this application. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of this application. Therefore, this application is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A disposable filtration centrifugal separation device, characterized in that, It includes a front chamber, a filter section, and a rear chamber, wherein the front chamber and the first end of the filter section are threadedly connected, and the rear chamber and the second end of the filter section are threadedly connected; it also includes a cover, which is threadedly connected to the front chamber. The front compartment is a cylindrical cavity; the filter section is a cylindrical cavity, with a first filter screen at the first end and a second filter screen at the second end, the first filter screen and the second filter screen being arranged in parallel; the upper part of the rear compartment is cylindrical and the lower part is a conical cavity.

2. The disposable filtration centrifugal separation device according to claim 1, characterized in that, The first filter screen and the first end of the filter section are smoothly transitioned by an arc-shaped surface; the second filter screen and the second end of the filter section are smoothly transitioned by an arc-shaped surface.

3. The disposable filtration centrifugal separation device according to claim 2, characterized in that, The filter section is integrally formed with the first filter screen and the second filter screen.

4. The disposable filtration centrifugal separation device according to claim 3, characterized in that, The area of ​​the first filter screen is smaller than the area of ​​the second filter screen.

5. The disposable filtration centrifugal separation device according to claim 4, characterized in that, The front compartment is provided with a first internal thread at its end, the first end of the filter section is provided with a first external thread, the second end of the filter section is provided with a second external thread, the first external thread and the second external thread are of different sizes, the rear compartment is provided with a second internal thread at its end, and the cover is provided with a third external thread at its end; the first internal thread is matched with the first external thread and the third external thread respectively, and the second internal thread and the second external thread are matched.

6. The disposable filtration centrifugal separation device according to claim 1, characterized in that, The other end of the lid has a groove.

7. The disposable filtration centrifugal separation device according to any one of claims 1-6, characterized in that, The front compartment, filter section, and rear compartment cover are each provided with a long, raised protrusion.

8. The disposable filtration centrifugal separation device according to claim 7, characterized in that, The front chamber, filter section, rear chamber, and cover are all transparent.

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