A water bath mixing device for cryopreservation of stem cells

Abstract

This application provides a water bath mixing device for cryopreservation of stem cells, comprising: an instrument housing; a water tank, which is positioned in a pre-reserved location on the instrument housing and has a support plate inside for holding several mixing modules; a water inlet pipe, which is connected to the water inlet of the instrument housing via a quick-release structure, with one end extending into the water tank, and a first control valve installed on the water inlet pipe for controlling the injection of water into the water tank; a drain pipe, which is connected to the drain outlet of the water tank via a quick-connect structure, with one end extending to the bottom of the water tank, and a second control valve installed on the drain pipe for controlling the drainage of water out of the water tank; and a sensor module, which is installed on the inner wall of the water tank for monitoring the temperature and level of the water inside the tank. This device has a high degree of integration, effectively reducing the need for frequent ice replenishment during use, and features reduced workload for medical personnel and high reliability in cell preparation.

Description

Technical Field

[0001] This application belongs to the field of stem cell cryopreservation preparations, and particularly relates to a water bath mixing device for stem cell cryopreservation preparations. Background Technology

[0002] During hematopoietic stem cell transplantation, when preparing the stem cell cryopreservation preparation, a certain amount of cryopreservation solution needs to be manually injected into the pre-packaged cryopreservation bags containing hematopoietic stem cells. The cryopreservation bags need to be continuously shaken in ice water to ensure they are thoroughly mixed.

[0003] Currently, the preparation process for stem cell cryopreservation formulations in hospitals is all done manually, which has the following main drawbacks: ① It requires manual replenishment of water to the water tank, which is cumbersome and inefficient. ② When operating manually, the ice water needs to be placed in a bucket, which cannot keep it cold and requires frequent replenishment of ice. Utility Model Content

[0004] In view of this, the present application aims to provide a water bath mixing device for stem cell cryopreservation preparations to solve at least one of the above-mentioned problems.

[0005] To achieve the above objectives, the technical solution of this application is implemented as follows: This application provides a water bath mixing device for cryopreservation of stem cell preparations, comprising: Instrument casing; A water tank is provided in a reserved placement position on the outer shell of the instrument, and a support plate for holding several shaking modules is provided inside the water tank. The water inlet pipe is installed at the water inlet of the instrument housing via a quick-release structure, and one end of the water inlet pipe extends into the water tank. The water inlet pipe is also equipped with a first control valve for controlling the water inlet pipe to inject water into the water tank. A drainage pipe is provided at the drain outlet of the water tank via a quick-connect structure, and one end of the drainage pipe extends to the bottom of the water tank. A second control valve is installed on the drainage pipe to control the drainage of water from the water tank to the outside. A sensor module is installed on the inner wall of the water tank to monitor the temperature and level of the water in the tank.

[0006] Furthermore, the support plate is embedded in the water tank, and the support plate is provided with a number of inwardly recessed cavities at intervals; The bottom of the support plate is provided with multiple magnet fixing plates, and multiple magnets are provided on the magnet fixing plates. The cavity is provided with through holes corresponding to the magnets. The bottom end of the shaking module passes through the through holes and is attracted to the magnets.

[0007] Furthermore, a water leakage process hole is also provided inside the cavity; The support plate has a drainage hole on its raised edge, which is connected to the water leakage process hole, and one end of the drainage pipe is inserted into the drainage hole.

[0008] Furthermore, the quick-connect structure includes a second quick-connect connector, one end of which is connected to a first drain bend, the first drain bend being inserted into the drain hole, and the other end of which is connected to a flange mounting connector installed at the drain outlet. The flange mounting connector is connected to a second control valve, and the second control valve is connected to the second drain bend to drain water from the tank.

[0009] Furthermore, a waterproof gasket is installed between the flange mounting joint and the inner wall of the water tank.

[0010] Furthermore, the second control valve is a manual ball valve.

[0011] Furthermore, the quick-release structure includes a first quick-connect fitting installed inside the water inlet. One end of the first quick-connect fitting is connected to the water inlet pipe, and the other end is connected to the first control valve. The other end of the first control valve is connected to the water source through a connecting pipe.

[0012] Furthermore, the first control valve is a solenoid ball valve.

[0013] Furthermore, the sensor module includes two sets of liquid level and temperature sensors, and the two sets of liquid level and temperature sensors are set at different heights; The liquid level and temperature sensor and the sensor wiring harness connected to the sensor are fixed to the inner wall of the water tank by pipe clamps. The sensor wiring harness is electrically connected to the internal equipment of the instrument through the cable sleeve provided in the water tank.

[0014] Furthermore, the outside of the water tank is also wrapped with insulating cotton.

[0015] Compared with the prior art, the water bath mixing device for stem cell cryopreservation preparations described in this application has the following beneficial effects: (1) The stem cell cryopreservation preparation water bath mixing device described in this application can realize automatic water replenishment and temperature monitoring, realize constant temperature and constant liquid level water bath mixing, effectively improve the efficient preparation of stem cell cryopreservation preparation, and at the same time, reduce the frequent addition of ice during use and reduce the workload of medical staff. (2) The stem cell cryopreservation preparation water bath mixing device described in this application has the characteristics of high integration, compact structure and low economic cost. Each part is easy to disassemble, can be easily replaced and maintained, and the overall structure has a high fault tolerance rate. Attached Figure Description

[0016] The accompanying drawings, which form part of this application, are used to provide a further understanding of this application. The illustrative embodiments and descriptions of this application are used to explain this application and do not constitute an undue limitation of this application. In the drawings: Figure 1 This is a schematic diagram of a water bath mixing device for stem cell cryopreservation preparations as described in an embodiment of this application. Figure 2 This is a schematic diagram showing some details of the water bath mixing device for stem cell cryopreservation preparations described in an embodiment of this application. Figure 3 This is a schematic diagram of the water tank structure described in the embodiments of this application; Figure 4 This is a schematic diagram of the first angle structure of the support plate described in an embodiment of this application; Figure 5 This is a schematic diagram of the second angle structure of the support plate described in the embodiment of this application; Figure 6 This is a cross-sectional view of the water inlet pipe described in the embodiment of this application; Figure 7 This is a cross-sectional view of the drainage pipeline described in the embodiment of this application; Figure 8 This is a schematic diagram of the sensor module structure described in an embodiment of this application.

[0017] Explanation of reference numerals in the attached figures: 1-Instrument housing; 2-Water tank; 201-Magnet fixing plate; 202-Magnet; 3-Support plate; 301-Cavity; 302-Fixing hole; 303-Through hole; 304-Water leakage process hole; 305-Drain hole; 4-Shaking module; 5-Water inlet pipe; 6-Connecting pipe; 7-First quick-connect connector; 8-First control valve; 9-First drain bend; 10-Second drain bend; 11-Second quick-connect connector; 12-Flange mounting connector; 13-Waterproof gasket; 14-Second control valve; 15-Sensor module; 1501-Liquid level and temperature sensor; 1502-Pipe clamp; 1503-Sensor wiring harness; 1504-Wire sheath; 1505-Pipe sheath; 16-Insulation cotton. Detailed Implementation

[0018] To make the objectives, technical solutions, and advantages of this application clearer, the following detailed description is provided in conjunction with specific embodiments and the accompanying drawings.

[0019] It should be noted that, unless otherwise defined, the technical or scientific terms used in the embodiments of this application should have the ordinary meaning understood by one of ordinary skill in the art to which this application pertains. The terms "first," "second," and similar terms used in the embodiments of this application do not indicate any order, quantity, or importance, but are merely used to distinguish different components. Terms such as "comprising" or "including" mean that the element or object preceding the word covers the element or object listed after the word and its equivalents, but does not exclude other elements or objects. Terms such as "connected" or "linked" are not limited to physical or mechanical connections, but can refer to any connection. This includes electrical connections, whether direct or indirect. Terms like "up," "down," "left," and "right" are used only to indicate relative positional relationships; these relative relationships may change if the absolute position of the object being described changes.

[0020] Please see Figure 1 and Figure 2 As shown, this embodiment provides a water bath mixing device for cryopreservation of stem cell preparations, comprising: Instrument casing 1; Water tank 2 is set in a reserved placement position on the instrument housing 1, and a support plate 3 is provided inside the water tank 2 for holding several shaking modules 4 (it should be noted that the existing shaking module 4 is used in this embodiment, and there is no technical improvement to the shaking module 4, so it will not be described in detail). The water inlet pipe is installed at the water inlet of the instrument housing 1 via a quick-release structure, and one end of the water inlet pipe extends into the water tank 2. The water inlet pipe is also equipped with a first control valve 8, which is used to control the water inlet pipe to inject water into the water tank 2. The drain pipe is installed at the drain outlet of the water tank 2 via a quick-connect structure, and one end of the drain pipe extends to the bottom of the water tank 2. A second control valve 14 is installed on the drain pipe to control the drain pipe to drain water out of the water tank 2. Sensor module 15 is installed on the inner wall of water tank 2 and is used to monitor the temperature and level of water in water tank 2.

[0021] The mixing device described in this embodiment can automatically replenish water and monitor temperature through the sensor module 15 installed in the water tank 2, achieving constant temperature and constant liquid level water bath mixing, effectively improving the efficient preparation of stem cell cryopreservation preparations. At the same time, it can reduce the need for frequent ice addition during use and reduce the workload of medical staff.

[0022] Meanwhile, the device features high integration, compact structure and low cost. Each part is easy to disassemble, can be easily replaced and maintained, and the overall structure has a high fault tolerance rate.

[0023] In some embodiments, the support plate 3 is embedded in the water tank 2, and the support plate 3 is provided with a plurality of inwardly recessed cavities 301 at intervals. The bottom of the support plate 3 is provided with multiple magnet fixing plates 201, and multiple magnets 202 are provided on the magnet fixing plates 201. The cavity 301 is provided with through holes 303 corresponding to the magnets 202. The bottom end of the shaking module 4 passes through the through holes 303 and attracts the magnets 202.

[0024] Specifically, in this embodiment, such as Figures 3 to 5 As shown, the support plate 3 is fitted into the water tank 2 by an interference fit, and six cavities 301 are arranged on the support plate 3 to hold six shaking modules 4 (it should be noted that the number of cavities and shaking modules is not specifically limited in this embodiment, and can be flexibly set according to the actual situation); in addition, in this embodiment, multiple fixing holes 302 are opened on the support plate 3, and fastening screws pass through the fixing holes 302 to fix the magnet fixing plate 201 to the bottom of the support plate 3, and magnets 202 are set on the magnet fixing plate 201 (the magnets 202 in this embodiment are plastic-coated magnets). The bottom end of the shaking module 4 passes through the through hole 303 provided on the support plate 3 and is attracted to the magnet 202, which makes it convenient for medical staff to take the shaking module 4 out of the water tank 2. The whole operation process is simple and easy to operate.

[0025] In some embodiments, the cavity 301 is provided with a plurality of water leakage process holes 304 around its periphery; The support plate 3 has a drainage hole 305 on its raised edge (it should be noted that the other side of the raised edge is hollowed out). The drainage hole 305 is connected to the water leakage process hole 304, and one end of the drainage pipe is inserted into the drainage hole 305.

[0026] Specifically, in this embodiment, such as Figure 4 and Figure 5 As shown, by opening a water leakage process hole 304 in the cavity 301 and keeping it connected with the drainage hole 305, the water in the water tank 2 can be completely drained to prevent water accumulation in the water tank 2.

[0027] In some embodiments, the quick-release structure includes a first quick-connect connector 7 installed in the water inlet. One end of the first quick-connect connector 7 is connected to the water inlet pipe 5, and the other end is connected to the first control valve 8. The other end of the first control valve 8 is connected to the water source through the connecting pipe 6. The first control valve 8 is an electromagnetic ball valve.

[0028] Specifically, in this embodiment, such as Figure 6As shown, the water inlet adopts a quick-release structure. The electromagnetic ball valve and the first quick-connect connector 7 are fixed on the instrument housing 1, which increases the stability of quick-connection, reduces the possibility of water leakage due to suspension, and is placed on the side and rear of the instrument to reduce contact with people. It can also quickly disassemble and assemble the water inlet pipe to achieve the purpose of safety and reliability. In addition, inside the instrument, the water inlet pipe 5 passes through the water tank 2 and the insulation cotton 16 to realize the quick-connection function. The quick-connect connector is partially hidden inside the instrument, which effectively prevents personnel from accidentally touching and operating it.

[0029] This embodiment features a quick-release structure, so when the water tank 2 needs to be disinfected or disassembled, only the water inlet pipe needs to be disassembled by hand, without disassembling other water inlet structures.

[0030] In some embodiments, the quick-connect structure includes a second quick-connect connector 11, one end of which is connected to a first drain bend 9, which is correspondingly inserted into a drain hole 305. The other end of the second quick-connect connector 11 is connected to a flange mounting connector 12 installed at the drain outlet. The flange mounting connector 12 is connected to a second control valve 14, which is connected to a second drain bend 10 to drain water from the water tank 2. The second control valve 14 is a manual ball valve. A waterproof gasket 13 is also installed between the flange mounting joint 12 and the inner wall of the water tank 2.

[0031] Specifically, in this embodiment, such as Figure 7 As shown, by setting a quick-connect structure at the drain outlet of the water tank 2, and using the cooperation between the quick-connect connector and the flange mounting connector 12, the first drain bend 9 is located inside the water tank 2, the second drain bend 10 is located outside the water tank 2, and is used in conjunction with the manual ball valve. In addition, a waterproof gasket 13 is used to achieve waterproofing of the end face of the water tank 2 by fastening the waterproof gasket 13 to the end face of the water tank 2.

[0032] Based on the drainage process hole 304 opened on the support plate 3, the drainage bend in the water tank 2 is connected to the bottom of the water tank 2 through the drainage hole 305 of the water tank 2. According to the principle of connection, the water in the water tank 2 is drained clean, which prepares for disassembly and disinfection. It has the characteristics of being economical and reliable, simple to install, and having a strong practicality in preparation process.

[0033] In some embodiments, the sensor module 15 includes two sets of liquid level and temperature sensors 1501, which are set at different heights. The liquid level and temperature sensor 1501 and the sensor wiring harness 1503 connected to the sensor are both fixed to the inner wall of the water tank 2 by the pipe clamp 1502. The sensor wiring harness 1503 is electrically connected to the internal equipment of the instrument through the cable sheath 1504 set in the water tank 2.

[0034] Specifically, in this embodiment, such as Figure 8As shown, by setting two sets of liquid level and temperature sensors 1501, one of which is installed at 1 / 2 of the distance from the bottom of the water tank 2 and the other is installed at 1 / 3 of the distance from the bottom of the water tank 2, the two liquid level and temperature sensors 1501 are installed in the core temperature control area of ​​the water bath mixing device, which can effectively and accurately measure the water bath temperature to ensure that the water bath temperature meets the preparation process requirements.

[0035] In addition, the liquid level temperature sensor 1501 is fixed to the water tank 2 through the pipe clamp 1502 (it should be noted that the pipe clamp 1502 in this embodiment is provided with a PA material protective tube 1505. By adding the protective tube 1505 structure, the effect of simulating suspension in water temperature measurement is achieved, and the accuracy of water temperature measurement is increased)). The liquid level temperature sensor 1501 detects the liquid level signal in the water tank 2. The sensor wiring harness 1503 enters the instrument through the protective sleeve 1504 (maintaining electrical connection with the host computer inside the instrument). When the liquid level has not reached the trigger liquid level temperature sensor 1501, the electromagnetic ball valve controls the automatic water filling. When the liquid level requirement is reached, the liquid level temperature sensor 1501 is triggered, and the water filling is automatically stopped.

[0036] Based on the temperature feedback from the liquid level and temperature sensor 1501, the host computer prompts the addition of ice. When the temperature and liquid level reach the required levels, it controls the automatic water supply and prompts the addition of ice, forming a negative feedback mechanism for temperature and liquid level. When draining, the water in the water tank 2 is drained using a manual ball valve to completely drain the water in the water tank 2.

[0037] It should be noted that this embodiment only innovates the technology of the device structure. The logic control of the sensor part is not the innovation of this patent. Moreover, the liquid level and temperature monitoring through the liquid level and temperature sensor is a conventional technical means in this field, so it will not be described in detail.

[0038] In some embodiments, the outside of the water tank 2 is also wrapped with heat insulation cotton 16. In this embodiment, by setting heat insulation cotton 16 on the outer wall of the water tank 2, the heat loss of the water bath in the water tank 2 can be reduced, thereby effectively reducing the frequency of manual ice replenishment.

[0039] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of this utility model, and not to limit it. Although the utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some or all of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of this utility model, and they should all be covered within the scope of the claims and specification of this utility model.

[0040] The embodiments of this application are intended to cover all such substitutions, modifications, and variations that fall within the broad scope of the appended claims. Therefore, any omissions, modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the embodiments of this application should be included within the protection scope of this application.

Claims

1. A water bath mixing device for cryopreservation of stem cell preparations, characterized in that, include: Instrument casing; A water tank is provided in a reserved placement position on the outer shell of the instrument, and a support plate for holding several shaking modules is provided inside the water tank. The water inlet pipe is installed at the water inlet of the instrument housing via a quick-release structure, and one end of the water inlet pipe extends into the water tank. The water inlet pipe is also equipped with a first control valve for controlling the water inlet pipe to inject water into the water tank. A drainage pipe is provided at the drain outlet of the water tank via a quick-connect structure, and one end of the drainage pipe extends to the bottom of the water tank. A second control valve is installed on the drainage pipe to control the drainage of water from the water tank to the outside. A sensor module is installed on the inner wall of the water tank to monitor the temperature and level of the water in the tank.

2. The water bath mixing device for stem cell cryopreservation preparation according to claim 1, characterized in that: The support plate is installed into the water tank, and the support plate is provided with a number of inwardly recessed cavities at intervals. The bottom of the support plate is provided with multiple magnet fixing plates, and multiple magnets are provided on the magnet fixing plates. The cavity is provided with through holes corresponding to the magnets. The bottom end of the shaking module passes through the through holes and is attracted to the magnets.

3. The water bath mixing device for stem cell cryopreservation preparation according to claim 2, characterized in that: The cavity is also provided with a water leakage process hole; The support plate has a drainage hole on its raised edge, which is connected to the water leakage process hole, and one end of the drainage pipe is inserted into the drainage hole.

4. The water bath mixing device for stem cell cryopreservation preparation according to claim 3, characterized in that: The quick-connect structure includes a second quick-connect connector. One end of the second quick-connect connector is connected to a first drain bend, which is inserted into the drain hole. The other end of the second quick-connect connector is connected to a flange mounting connector installed at the drain outlet. The flange mounting connector is connected to a second control valve, which is connected to the second drain bend to drain water from the tank.

5. The water bath mixing device for stem cell cryopreservation preparation according to claim 4, characterized in that: A waterproof gasket is also installed between the flange mounting joint and the inner wall of the water tank.

6. The water bath mixing device for stem cell cryopreservation preparation according to claim 4, characterized in that: The second control valve is a manual ball valve.

7. The water bath mixing device for stem cell cryopreservation preparation according to claim 1, characterized in that: The quick-release structure includes a first quick-connect fitting installed inside the water inlet. One end of the first quick-connect fitting is connected to the water inlet pipe, and the other end is connected to the first control valve. The other end of the first control valve is connected to the water source through a connecting pipe.

8. The water bath mixing device for stem cell cryopreservation preparation according to claim 7, characterized in that: The first control valve is a solenoid ball valve.

9. The water bath mixing device for stem cell cryopreservation preparation according to claim 1, characterized in that: The sensor module includes two sets of liquid level and temperature sensors, and the two sets of liquid level and temperature sensors are set at different heights. The liquid level and temperature sensor and the sensor wiring harness connected to the sensor are fixed to the inner wall of the water tank by pipe clamps. The sensor wiring harness is electrically connected to the internal equipment of the instrument through the cable sleeve provided in the water tank.

10. The water bath mixing device for stem cell cryopreservation preparation according to claim 1, characterized in that: The outside of the water tank is also wrapped with insulating cotton.

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