An automatic bacterial liquid dispensing workstation

Abstract

The utility model discloses a kind of bacterial liquid subpackaging automation workstations, belong to biological product automation equipment technical field.The workstation includes rack, cryopreservation box temperature control, automatic mixing uniform refrigeration, peristaltic pump, automatic opening and closing cover subpackaging and automatic transfer six big modules;Rack module is equipped with FFU clean airflow and transparent window, cryopreservation box temperature control module maintains bacterial liquid activity, automatic mixing uniform refrigeration module realizes bacterial liquid mixing uniform and cryogenic preservation, peristaltic pump accurately transports bacterial liquid, automatic opening and closing cover subpackaging module completes test tube opening cover, subpackaging and closing cover, and automatic transfer module realizes test tube clean transfer and is equipped with nitrogen pollution prevention.The utility model realizes bacterial liquid subpackaging whole-process automation, improves efficiency and precision, reduces pollution risk, compact structure, convenient operation, applicable to biological product production and laboratory bacterial liquid processing scene.

Description

Technical Field

[0001] This utility model belongs to the field of automated equipment technology for biological products, specifically relating to an integrated workstation that integrates functions such as temperature-controlled preservation of bacterial culture, automatic mixing and cooling, precise delivery, test tube opening and closing and dispensing, and sample transfer. Background Technology

[0002] In fields such as biopharmaceutical production and microbial experiments, the dispensing process for bacterial suspensions involves multiple steps, including cryopreservation and thawing, mixing and cooling, precise dispensing, and test tube sealing. Each step requires strict temperature control to ensure bacterial activity. Current technologies rely on independent equipment for each step: cryopreserved bacterial suspensions are stored in dedicated temperature-controlled cryovials; mixing and cooling depend on separate stirring and cooling devices; dispensing is done manually or with a simple pump; opening and closing test tube caps requires manual assistance; and sample transfer between multiple devices relies on manual intervention.

[0003] This decentralized operation model has significant drawbacks: 1. Inefficient: Manual transfer and switching between multiple devices are time-consuming and labor-intensive, which cannot meet the needs of high-throughput dispensing, and the activity of bacterial culture is easily affected by delays in operation. 2. Insufficient precision: Manual dispensing results in large errors and poor consistency in bacterial dosage, affecting the quality of subsequent experiments or production; 3. High risk of contamination: The bacterial solution is exposed to the air for a long time and is frequently handled by humans, making it susceptible to contamination from the external environment; 4. High cost and space occupation: Multiple independent devices need to be purchased, installed and maintained separately, resulting in high equipment costs and occupying a large amount of laboratory or production space; 5. Temperature control failure: The temperature cannot be continuously controlled during freezing, transportation, and packaging, which can easily lead to a decrease in bacterial activity.

[0004] Although some automated dispensing modules exist in existing technologies, most can only automate a single step (such as opening and closing the lid or dispensing), failing to form a closed-loop process of "temperature control-mixing-transfer-dispensing-transfer." Furthermore, the modules have poor compatibility, making it difficult to adapt to the specific needs of bacterial culture dispensing. Therefore, developing a compact, functionally integrated, and fully automated bacterial culture dispensing workstation is key to addressing the pain points of existing technologies. Summary of the Invention

[0005] This invention aims to overcome the shortcomings of existing bacterial culture dispensing processes, such as fragmentation, low automation, poor accuracy, and high risk of contamination, by providing an automated workstation for bacterial culture dispensing. This workstation integrates six core modules: a rack, cryopreservation box temperature control, automatic mixing and cooling, peristaltic pump delivery, automatic cap opening and closing for dispensing, and automatic transfer. It achieves fully automated operation of the entire process from cryopreservation and thawing to test tube packaging, improving dispensing efficiency and accuracy, reducing contamination risks, and saving labor and space costs.

[0006] The above-mentioned objective of this utility model is achieved through the following technical solution: An automated workstation for dispensing bacterial culture includes a rack module 1, a cryopreservation box temperature control module 2, an automatic mixing and automatic cooling module 3, a peristaltic pump module 4, an automatic lid opening and closing and automatic dispensing module 5, and an automatic transfer module 6. These modules work together to automate the entire bacterial culture dispensing process. The specific structure is as follows: Rack Module 1: This serves as the load-bearing foundation for the workstation, employing a stainless steel and aluminum alloy composite frame structure. It includes the bottom shell 1.1, front sealing plate 1.2, base plate 1.3, front panel 1.4, right panel 1.5, left panel 1.6, FFU mounting plate 1.7, rear panel 1.8, ventilation duct 1.9, FFU 1.10, touch screen 1.11, hinge 1.12, nitrogen spring 1.13, handle 1.14, start button 1.15, emergency stop button 1.16, and foot pads 1.17. The base plate 1.3 is used to fix each functional module. The FFU 1.10 (fan filter unit) is fixed to the top through the FFU mounting plate 1.7, which, together with the ventilation duct 1.9, forms a clean airflow environment to avoid bacterial contamination. The touch screen 1.11, start button 1.15, and emergency stop button 1.16 are integrated into the front cover plate 1.2 for parameter setting, process start and emergency stop; A transparent window is provided on the front panel 1.4. The transparent window is connected to the front panel 1.4 by a hinge 1.12 and is opened and closed by a nitrogen spring 1.13. The transparent window is provided with a handle 1.14 for easy operation. The feet 1.17 are located at the bottom of the overall frame module 1 for leveling and shock absorption.

[0007] Cryopreservation box temperature control module 2: fixed to one side of the base plate 1.3 of the frame module 1, used for constant temperature preservation and recovery of bacterial liquid cryopreservation boxes, including mounting frame 2.1, aluminum block cover 2.2, cryopreservation box 2.3 and cryopreservation box lid 2.4; Mounting bracket 2.1 is made of aluminum alloy and is used to fix the aluminum block cover 2.2; The aluminum block cover 2.2 has built-in heating / cooling elements and a temperature sensor, with a temperature control range of -20℃ to 37℃ and a temperature accuracy of ±0.5℃. It is used to wrap the cryopreservation box 2.3 and maintain a constant temperature. The cryopreservation box 2.3 is used to place containers for dispensing bacterial solutions. The cryopreservation box lid 2.4 is sealed to prevent dust and avoid contamination of the bacterial solutions.

[0008] Automatic mixing and automatic cooling module 3: fixed in the middle of the base plate 1.3 of the frame module 1, used for automatic mixing and low-temperature preservation of bacterial solution, including magnetic stirring cover 3.1, protective cover 3.2 and bacterial solution bottle 3.3; The magnetic stirring housing 3.1 has a built-in magnetic stirring component and a semiconductor cooling element. The stirring component drives the magnetic particles in the bacterial liquid bottle 3.3 to rotate, so as to achieve uniform mixing of the bacterial liquid. The stirring speed is adjustable from 50 to 500 rpm. The semiconductor cooling element is regulated by a temperature sensor, with a temperature control range of 4℃-25℃, ensuring that the bacterial solution maintains its activity during the mixing process; The protective cover 3.2 is made of transparent PC material and is placed on the outside of the bacterial culture bottle 3.3 to prevent dust and facilitate observation of the mixing status.

[0009] Peristaltic pump module 4: Fixed to the base plate 1.3 of the frame module 1 near the side of the automatic mixing and automatic cooling module 3, used for precise delivery of bacterial solution, including tube clamp block 4.1, pump head housing 4.2 and peristaltic pump 4.3; The peristaltic pump 4.3 is a precision metering type with an adjustable flow rate of 10-100 mL / min and an accuracy of ±2%. It is fixed by the pump head housing 4.2. The tube clamp 4.1 is set on one side of the pump head housing 4.2 to fix the delivery hose. One end of the hose is connected to the bacterial liquid bottle 3.3 of the automatic mixing and automatic cooling module 3, and the other end is connected to the plate connector 5.13 of the automatic opening and closing cap and automatic dispensing module 5, so as to realize the closed delivery of bacterial liquid from the mixing container to the test tube.

[0010] Automatic opening and closing cap and automatic dispensing module 5: Fixed to the other side of the base plate 1.3 of the frame module 1, used for automatic opening of test tubes, dispensing of bacterial solution and automatic closing of caps, including opening and closing cap housing 5.1, rotating gripper 5.2, rotating gripper finger 5.3, adhesive block 5.4, waste liquid tank 5.5, parallel clamping plate one 5.6, parallel clamping plate two 5.7, clamping block 5.8, gripper connecting plate 5.9, parallel gripper 5.10, rotating rod 5.11, rotating plate 5.12 and plate connector 5.13; The rotating jaw 5.2 drives the rotating gripper finger 5.3 to clamp the test tube body. The rubber block 5.4 (elastic rubber material) is located inside the rotating gripper finger 5.3, which fits against the test tube wall to avoid injury and improve stability. Parallel grippers 5.10 are fixed by gripper connecting plates 5.9, driving parallel clamping plates 5.6 and 5.7 to open and close. Clamping blocks 5.8 (silicone material) hold the test tube caps, and the opening / closing of the caps is completed in conjunction with the rotation of rotating grippers 5.2. The rotating rod 5.11 connects to the rotating plate 5.12 and drives the rotating plate 5.12 to rotate. The through-plate connector 5.13 connects to the delivery hose of the peristaltic pump module 4 to accurately inject the bacterial solution into the test tube. Waste liquid tank 5.5 is fixed to one side of switch cover 5.1 and is used to collect waste liquid that may drip during the dispensing process to avoid contamination.

[0011] Automatic transfer module 6: Installed on the base plate 1.3 of the frame module 1, it is used to transfer test tubes between the cryopreservation box temperature control module 2 and the automatic opening and closing lid and automatic dispensing module 5. It includes a robotic arm mounting base 6.1, a robot 6.2, a gripper flange 6.3, a gripper 6.4, a nitrogen needle support 6.5, gripper fingers 6.6 and a nitrogen needle 6.7. The robotic arm mounting base 6.1 is fixed on the base plate 1.3, and the robot 6.2 is a multi-degree-of-freedom robotic arm (at least 4 axes) with a repeatability accuracy of ±0.1mm; The gripper 6.4 is connected to the end of the robot 6.2 via the gripper flange 6.3, and the gripper fingers 6.6 (with anti-slip texture on the inside) are used to grip the test tube; The nitrogen needle holder 6.5 is fixed to one side of the clamp 6.4, and the nitrogen needle 6.7 is used to spray a small amount of nitrogen into the test tube opening during the transfer process to form a local clean environment and further reduce the risk of contamination.

[0012] The working principle of this utility model: Set the dispensing parameters (such as bacterial culture dosage, temperature control temperature, and stirring speed) via the touchscreen 1.11 on the front cover 1.2. After starting the workstation by pressing the start button 1.15, it will run automatically according to the following process: 1. Preparation of bacterial solution: The aluminum block cover 2.2 of the cryopreservation box temperature control module 2 starts heating / cooling to adjust the bacterial solution in the cryopreservation box 2.3 to the preset temperature (such as 37℃ for thawing or 4℃ for storage); the robot 6.2 of the automatic transfer module 6 uses gripper fingers 6.6 to grab empty test tubes and transfer them from the cryopreservation box 2.3 to the gripping station of the rotating gripper 5.2 of the automatic opening and closing lid and automatic dispensing module 5; 2. Automatic lid opening: The rotating gripper 5.2 of the automatic lid opening and automatic dispensing module 5 drives the rotating gripper finger 5.3 to clamp the test tube, and the rubber block 5.4 adheres to the test tube wall to prevent slippage; the parallel gripper 5.10 drives the parallel clamping plate one 5.6 and parallel clamping plate two 5.7 to close, and the clamping block 5.8 clamps the test tube lid; the rotating gripper 5.2 drives the test tube body to rotate, so that the test tube lid is separated from the body, and the robot 6.2 grabs the test tube lid and stores it temporarily; 3. Mixing and conveying of bacterial solution: The magnetic stirring component of the automatic mixing and automatic cooling module 3 is activated, driving the magnetic force in the bacterial solution bottle 3.3 to rotate and mix the bacterial solution. The semiconductor cooling element maintains the temperature of the bacterial solution simultaneously. The peristaltic pump module 4 is activated, and the conveying hose, under the limit of the tube clamp 4.1, conveys the bacterial solution from the bacterial solution bottle 3.3 to the plate connector 5.13 of the automatic opening and closing cap and automatic dispensing module 5. 4. Automatic dispensing: The rotating rod 5.11 drives the rotating plate 5.12 to rotate, the plate connector 5.13 is aligned with the test tube opening, and the bacterial solution is injected according to the preset dosage. The waste liquid dripping during the dispensing process is collected by the waste liquid tank 5.5 on one side of the switch cover 5.1. 5. Automatic cap closing: Robot 6.2 transfers the temporarily stored test tube cap to the top of the test tube, the parallel gripper 5.10 clamps the test tube cap and slides it onto the test tube body, and the rotating gripper 5.2 rotates in the opposite direction to tighten the test tube cap; 6. Finished product transfer: The nitrogen needle 6.7 of the automatic transfer module 6 sprays a small amount of nitrogen into the test tube opening, and the robot 6.2 picks up the packaged test tube and transfers it back to the cryopreservation box 2.3 of the cryopreservation box temperature control module 2 for storage, completing the entire packaging process; if an abnormality occurs, the emergency stop button 1.16 can be pressed to stop the machine in an emergency.

[0013] Compared with the prior art, the present invention has the following beneficial effects: 1. Fully automated process with significantly improved efficiency: Integrating the entire process of "temperature control - transfer - opening and closing the lid - mixing - dispensing - storage", no manual intervention is required, the high-throughput processing capacity is increased by more than 50%, and labor costs and time are greatly saved; 2. Precise control and stable quality: The peristaltic pump provides precise metering (error ±1%), and the temperature control of the cryopreservation box is linked with the mixing and cooling module to ensure the consistency of bacterial activity and dispensing dosage. 3. Multiple anti-contamination design: FFU clean airflow, nitrogen needle local nitrogen protection, waste liquid collection tank, combined with closed delivery pipeline, form a "macro + micro" dual anti-contamination system to minimize the risk of bacterial liquid contamination; 4. Compact structure and easy operation: All modules are integrated into the same rack, occupying only 1 / 3 of the space of traditional distributed equipment; the transparent window on the front panel makes it easy to observe the internal process, and the touch screen provides visual operation, allowing novices to get started quickly; 5. Safe, reliable, and highly compatible: The emergency stop button and protective structure ensure safe operation. It is compatible with various test tube sizes such as 10mL, 15mL, and 20mL. The cryopreservation box is compatible with bacterial culture containers of different capacities, making it widely applicable. Attached Figure Description

[0014] The accompanying drawings, which are included to provide a further understanding of the present invention and form part of this application, illustrate exemplary embodiments of the present invention and, together with their description, serve to explain the present invention, but do not constitute an undue limitation thereof. In the drawings: Figure 1 This is a schematic diagram of the overall structure of this utility model.

[0015] Figure 2 This is a structural schematic diagram of the rack module in this utility model.

[0016] Figure 3 This is a structural schematic diagram of the temperature control module of the cryopreservation box in this utility model.

[0017] Figure 4 This is a schematic diagram of the automatic mixing and automatic cooling module in this utility model.

[0018] Figure 5 This is a structural schematic diagram of the peristaltic pump module in this utility model.

[0019] Figure 6 This is a structural schematic diagram of the automatic cover opening and closing module and the automatic dispensing module in this utility model.

[0020] Figure 7 This is a structural schematic diagram of the automatic transfer module in this utility model.

[0021] Explanation of reference numerals in the attached figures: 1-Rack module, 1.1-Bottom shell, 1.2-Front end plate, 1.3-Bottom plate, 1.4-Front panel, 1.5-Right panel, 1.6-Left panel, 1.7-FFU mounting plate, 1.8-Rear panel, 1.9-Ventilation duct, 1.10-FFU, 1.11-Touch screen, 1.12-Hinge, 1.13-Nitrogen spring, 1.14-Handle, 1.15-Start button, 1.16-Emergency stop button, 1.17-Foot pads; 2-Temperature control module for cryopreservation box; 2.1-Mounting bracket; 2.2-Aluminum block cover; 2.3-Cryopreservation box; 2.4-Cryopreservation box lid; 3-Automatic mixing and automatic cooling module; 3.1-Magnetic stirring cover; 3.2-Protective cover; 3.3-Bacterial liquid bottle; 4-Peristaltic pump module, 4.1-Tube clamp block, 4.2-Pump head housing, 4.3-Peristaltic pump; 5-Automatic opening and closing cover and automatic dispensing module; 5.1-Opening cover housing; 5.2-Rotating gripper; 5.3-Rotating gripper finger; 5.4-Glue block; 5.5-Waste liquid tank; 5.6-Parallel clamping plate one; 5.7-Parallel clamping plate two; 5.8-Clamping block; 5.9-Gripper connecting plate; 5.10-Parallel gripper; 5.11-Rotating rod; 5.12-Rotating plate; 5.13-Through plate connector. 6-Automatic transfer module, 6.1-Robot arm mounting base, 6.2-Robot, 6.3-Grip flange, 6.4-Grip, 6.5-Nitrogen needle bracket, 6.6-Grip finger, 6.7-Nitrogen needle. Detailed Implementation

[0022] To make the objectives, technical solutions, and advantages of this utility model clearer, the present utility model will be further described in detail below with reference to specific embodiments and accompanying drawings. It should be understood that these descriptions are merely exemplary and not intended to limit the scope of this utility model. Furthermore, descriptions of well-known structures and technologies are omitted in the following description to avoid unnecessarily obscuring the concept of this utility model.

[0023] Example: Automated workstation for dispensing bacterial solutions like Figure 1-7 As shown, the specific parameters and assembly details of each module of the automated bacterial culture dispensing workstation in this embodiment are as follows: Rack Module 1: The bottom shell 1.1, front sealing plate 1.2, and base plate 1.3 are all made of 304 stainless steel with a thickness of 2mm; the transparent window of the front panel 1.4 is made of 5mm thick acrylic sheet, connected to the front panel 1.4 by hinges 1.12, and nitrogen springs 1.13 assist in opening and closing the window; the FFU 1.10 has an air volume of 1200m³ / h and a filtration efficiency of H13; the touch screen 1.11 is a 10-inch industrial touch screen that supports Chinese and English switching; the feet 1.17 are made of rubber with an adjustable height range of 0-15mm, used for rack leveling and vibration reduction.

[0024] Cryopreservation box temperature control module 2: Mounting bracket 2.1 is made of 6061 aluminum alloy with a cross-sectional size of 40×40mm; aluminum block cover 2.2 houses a 100W heating element and a 50W TEC semiconductor cooling element, with a PT100 temperature sensor, a temperature control range of -20℃ to 37℃, and an accuracy of ±0.5℃; cryopreservation box 2.3 is made of polypropylene with a capacity of 24×15mL test tubes; cryopreservation box lid 2.4 is equipped with a silicone sealing ring with a sealing rating of IP54.

[0025] Automatic mixing and automatic cooling module 3: The magnetic stirring shell 3.1 is made of ABS material, with a built-in 24V DC magnetic stirring motor with an adjustable speed of 50-500rpm; the semiconductor cooling chip has a power of 50W and is equipped with aluminum heat sink fins; the bacterial liquid bottle 3.3 is made of 500mL high borosilicate glass with a silicone sealing plug; the protective cover 3.2 is made of 3mm thick transparent PC material and is connected to the magnetic stirring shell 3.1 by a buckle.

[0026] Peristaltic pump module 4: Peristaltic pump 4.3 flow range 10-100mL / min, accuracy ±2%; pump head housing 4.2 is made of ABS material, tubing clamp 4.1 is made of polytetrafluoroethylene material, compatible with Φ6×9mm food-grade silicone tubing, tubing pressure rating 0.6MPa.

[0027] Automatic opening and closing cover and automatic dispensing module 5: Rotary gripper 5.2 with a rotation angle of 0-360° and an adjustable clamping force of 50-200N; Parallel gripper 5.10 with a clamping stroke of 20mm; Rubber block 5.4 made of elastic rubber with a Shore hardness of 50, clamping block 5.8 made of silicone material with a Shore hardness of 40 and a thickness of 3mm; Waste liquid tank 5.5 made of polypropylene material with a capacity of 500mL and a detachable waste liquid box.

[0028] Automatic transfer module 6: The robotic arm mounting base 6.1 is made of stainless steel and is fixed to the base plate 1.3 by expansion bolts; the robot 6.2 is a 4-axis industrial robotic arm with a maximum load of 3kg and a repeatability of ±0.02mm; the gripper fingers 6.6 are made of aluminum alloy with a 0.5mm thick silicone anti-slip pad attached to the inside; the nitrogen needle 6.7 is made of 316L stainless steel with a hole diameter of 0.5mm, a nitrogen working pressure of 0.1MPa, and a flow rate of 50mL / min.

[0029] Work process: 1. Parameter settings: Set the bacterial solution dosage to 5mL, cryopreservation box temperature control to 37℃, mixing speed to 200rpm, and cooling temperature to 10℃ via the touch screen 1.11, and save the parameters; 2. Start-up: The cryopreservation box temperature control module 2 starts. After 30 minutes, the temperature of the bacterial solution in the cryopreservation box 2.3 reaches 37°C. The robot 6.2 of the automatic transfer module 6 picks up a 15mL empty test tube and transfers it to the rotating gripper 5.2 station of the automatic opening and closing cap module 5. 3. Opening and dispensing: Rotary jaw 5.2 clamps the test tube, parallel jaw 5.10 holds the test tube cap and rotates to open it; peristaltic pump 4.3 starts, 5mL of bacterial solution is injected into the test tube through the plate connector 5.13, and the waste liquid trough 5.5 collects the dripping waste liquid; 4. Closing and storage: Parallel gripper 5.10 moves the test tube cap onto the test tube, and rotating gripper 5.2 rotates in the opposite direction to close the cap; the nitrogen needle 6.7 of robot 6.2 sprays nitrogen gas, grabs the test tube and transfers it back to cryopreservation box 2.3, and the cryopreservation box temperature control module 2 switches to 4℃ for storage; 5. Batch processing: The equipment automatically repeats the above steps and completes the batch dispensing of 24 test tubes within 1 hour. The dispensing error is ≤ ±0.05mL, and the contamination rate is <0.1% after sterility testing. If pipeline blockage occurs, the emergency stop button 1.16 is triggered, and the equipment automatically cuts off power for protection.

[0030] The above description is merely an embodiment of this utility model and does not limit the patent scope of this utility model. Any equivalent structural or procedural transformations made based on the description and drawings of this utility model, or direct or indirect applications in other related technical fields, are similarly included within the patent protection scope of this utility model.

Claims

1. An automated workstation for dispensing bacterial solutions, characterized in that: It includes a rack module (1), a cryopreservation box temperature control module (2), an automatic mixing and automatic cooling module (3), a peristaltic pump module (4), an automatic lid opening and closing and automatic dispensing module (5), and an automatic transfer module (6). The rack module (1) includes a bottom shell (1.1), a front sealing plate (1.2), a base plate (1.3), a front panel (1.4), a right panel (1.5), a left panel (1.6), an FFU mounting plate (1.7), a rear panel (1.8), a ventilation duct (1.9), an FFU (1.10), a touch screen (1.11), a hinge (1.12), a nitrogen spring (1.13), a handle (1.14), a start button (1.15), an emergency stop button (1.16), and foot pads (1.17); the base plate (1.3) is used for fixing Each functional module has an FFU (1.10) fixed to the top via an FFU mounting plate (1.7), and a ventilation duct (1.9) connected to the FFU (1.10); a touch screen (1.11), a start button (1.15), and an emergency stop button (1.16) integrated into the front cover plate (1.2); a transparent window is provided on the front panel (1.4), which is connected to the front panel (1.4) via a hinge (1.12), and a nitrogen spring (1.13) assists in opening and closing; a handle (1.14) is provided on the transparent window; and foot pads (1.17) are provided at the bottom of the rack module (1). The cryopreservation box temperature control module (2) is fixed to one side of the base plate (1.3) and includes a mounting bracket (2.1), an aluminum block cover (2.2), a cryopreservation box (2.3), and a cryopreservation box lid (2.4). The mounting bracket (2.1) fixes the aluminum block cover (2.2), which has a built-in heating / cooling element and a temperature sensor. The cryopreservation box (2.3) is placed inside the aluminum block cover (2.2), and the cryopreservation box lid (2.4) covers the cryopreservation box (2.3). The automatic mixing and automatic cooling module (3) is fixed in the middle of the base plate (1.3) and includes a magnetic stirring shell (3.1), a protective cover (3.2) and a bacterial liquid bottle (3.3); the magnetic stirring shell (3.1) has a built-in magnetic stirring assembly and a semiconductor cooling element, the bacterial liquid bottle (3.3) is placed inside the magnetic stirring shell (3.1), and the protective cover (3.2) covers the outside of the bacterial liquid bottle (3.3); The peristaltic pump module (4) is fixed to the base plate (1.3) near the side of the automatic mixing and automatic cooling module (3), and includes a tube clamping block (4.1), a pump head housing (4.2) and a peristaltic pump (4.3); the peristaltic pump (4.3) is fixed by the pump head housing (4.2), the tube clamping block (4.1) is located on one side of the pump head housing (4.2), and the peristaltic pump (4.3) is connected to the bacterial liquid bottle (3.3) through a flexible tube. The automatic switch cover and automatic dispensing module (5) is fixed to the other side of the base plate (1.3), including a switch cover shell (5.1), a rotating gripper (5.2), a rotating gripper finger (5.3), a rubber block (5.4), a waste liquid tank (5.5), a parallel clamping plate one (5.6), a parallel clamping plate two (5.7), a clamping block (5.8), a gripper connecting plate (5.9), a parallel gripper (5.10), a rotating rod (5.11), a rotating plate (5.12), and a through-plate connector (5.13); the rotating gripper ( 5.2) Connect the rotating gripper (5.3), and the rubber block (5.4) is located inside the rotating gripper (5.3); the parallel gripper (5.10) is fixed by the gripper connecting plate (5.9), driving the parallel clamping plate one (5.6) and parallel clamping plate two (5.7) to open and close, and the clamping block (5.8) is located inside the clamping plate; the rotating rod (5.11) is connected to the rotating plate (5.12), and the through plate joint (5.13) is installed on the rotating plate (5.12); the waste liquid tank (5.5) is fixed to one side of the switch cover (5.1); The automatic transfer module (6) is installed on the base plate (1.3) and includes a robotic arm mounting base (6.1), a robot (6.2), a gripper flange (6.3), a gripper (6.4), a nitrogen needle bracket (6.5), gripper fingers (6.6), and a nitrogen needle (6.7). The robotic arm mounting base (6.1) is fixed to the base plate (1.3), and the robot (6.2) is installed on the robotic arm mounting base (6.1). The gripper (6.4) is connected to the robot (6.2) through the gripper flange (6.3), and the gripper fingers (6.6) are located on the gripper (6.4). The nitrogen needle bracket (6.5) is fixed to one side of the gripper (6.4), and the nitrogen needle (6.7) is installed on the nitrogen needle bracket (6.5).

2. The automated workstation for dispensing bacterial solutions according to claim 1, characterized in that: The aluminum block cover (2.2) of the cryopreservation box temperature control module (2) has a temperature control range of -20℃ to 37℃ and a temperature accuracy of ±0.5℃.

3. The automated workstation for dispensing bacterial solutions according to claim 1, characterized in that: The magnetic stirring component of the automatic mixing and automatic cooling module (3) has an adjustable speed of 50-500 rpm, and the temperature control range of the semiconductor cooling element is 4℃-25℃.

4. The automated workstation for dispensing bacterial solutions according to claim 1, characterized in that: The peristaltic pump (4.3) of the peristaltic pump module (4) is a precision metering type with a flow range of 10-100 mL / min and an accuracy of ±2%.

5. The automated workstation for dispensing bacterial solutions according to claim 1, characterized in that: The adhesive block (5.4) of the automatic opening and closing cover and automatic dispensing module (5) is made of elastic rubber, and the clamping block (5.8) is made of silicone.

6. The automated workstation for dispensing bacterial solutions according to claim 1, characterized in that: The robot (6.2) of the automatic transfer module (6) is a multi-degree-of-freedom robotic arm with at least 4 axes and a repeatability accuracy of ±0.1mm; the inner side of the gripper fingers (6.6) is provided with anti-slip texture.

7. The automated workstation for dispensing bacterial solutions according to claim 1, characterized in that: The FFU (1.10) filtration efficiency of the rack module (1) is ≥H13, and the transparent window of the front panel (1.4) is made of acrylic or PC material.

Images