A magnetic stand that can be fitted to the bottom of an EP tube

Abstract

The application relates to the field of biological separation, and specifically provides a magnetic frame which can be matched with the bottom shape of an EP tube, comprising: a support, first and second insertion hole gaps being arranged in the height direction of the support, the first and second insertion hole gaps being corresponding and matched, and being used for placing the EP tube; a magnet being arranged between the first and second insertion hole gaps and around the bottom outer periphery of the EP tube; an adjusting assembly being connected to the magnet and being used for adjusting the contact between the magnet and the side wall of the EP tube; wherein the ratio between the contact area of the magnet and the inner wall area of the magnet is between 90% and 100%. Through the cooperation between the support, the magnet and the adjusting assembly, the contact pressure between the magnet and the side wall of the EP tube can be flexibly adjusted, different specifications of the EP tube can be matched, the universality of the equipment is enhanced, the contact area ratio can reach 90%-100%, the magnetic field action range is greatly enhanced, the magnetic substance can be rapidly enriched at the bottom of the EP tube, and the separation efficiency is improved.

Description

Technical Field

[0001] This application relates to the field of bioseparation technology, specifically to a magnetic frame that can conform to the shape of the bottom of an EP tube. Background Technology

[0002] Currently, in experiments using magnetic beads to enrich target substances, the magnetic beads connected to the target substance in the EP tube are attracted to the inner wall of the EP tube by magnets on the magnetic rack to achieve separation of the magnetic beads from the solution. The experimenter can then remove the solution and retain the magnetic beads to carry out the next step of the experiment.

[0003] However, the current magnetic racks have the following drawbacks: the magnets of the existing magnetic racks only act on the EP tube in point contact or small-area block contact, which limits the range of magnetic field. Magnetic materials (such as magnetic beads) are prone to leave residues at the bottom of the tube, which not only makes it difficult to draw out the solution in the tube, but may also cause some magnetic beads to be sucked away and damaged. Secondly, the existing magnetic racks cannot be adapted to EP tubes with different inner diameters / heights. Therefore, when conducting experiments with EP tubes with different inner diameters / heights, it is necessary to replace them with special magnetic racks, which increases the equipment cost. Utility Model Content

[0004] To overcome the shortcomings of the prior art, this application provides a magnetic frame that can conform to the shape of the bottom of the EP tube, and the specific technical solution is as follows:

[0005] A magnetic frame that can conform to the shape of the bottom of an EP tube includes:

[0006] The bracket has a first insertion hole and a second insertion hole spaced apart along its height direction. The first insertion hole and the second insertion hole correspond to and cooperate with each other for placing the EP tube.

[0007] A magnet is located between the first insertion hole and the second insertion hole, and is arranged around the bottom outer periphery of the EP tube;

[0008] An adjustment component, connected to the magnet, is used to adjust the contact between the magnet and the sidewall of the EP tube;

[0009] The ratio between the contact area between the magnet and the EP tube and the inner wall area of ​​the magnet is between 90% and 100%.

[0010] In one specific embodiment, the line connecting the center of the first insertion hole and the center of the second insertion hole is a first straight line, which coincides with the axis of the EP tube; the inner wall of the magnet includes an arc-shaped inner wall, the center of which lies on the first straight line.

[0011] In one specific embodiment, the adjustment component includes a lifting component and an elastic element. The lifting component moves along the height direction of the bracket to cooperate with the magnet to adjust the height of the magnet.

[0012] One end of the elastic element is connected to the lifting assembly, and the other end of the elastic element is connected to the magnet, which is used to make the magnet abut against the side wall of the EP tube under the elastic force.

[0013] In one specific embodiment, the lifting assembly includes a lifting rod and a locking member.

[0014] The first end of the lifting rod passes through the bracket and moves along the height direction of the bracket; the second end of the lifting rod extends along the length direction of the bracket, and the magnet is disposed at the second end of the lifting rod; the lifting rod is used to adjust the height of the magnet when moving.

[0015] The locking member is movably disposed at the first end of the lifting rod, and the locking member is placed on the top of the bracket and abuts against the bracket to lock the position of the lifting rod.

[0016] In one specific embodiment, a threaded structure is provided between the locking member and the lifting rod, and the locking member is movably mounted on the lifting rod through the threaded structure.

[0017] In one specific embodiment, the first insertion port has a plurality of first insertion ports, which are spaced apart along the length direction of the bracket;

[0018] The second insertion port has multiple second insertion ports, which are spaced apart along the length of the bracket. The multiple second insertion ports correspond one-to-one with the multiple first insertion ports and cooperate to place multiple EP tubes.

[0019] In one specific embodiment, the magnet includes a claw magnet or a tile magnet.

[0020] In one specific embodiment, the bracket includes a base and a bottom plate, the bottom plate being disposed at the bottom of the base, the first insertion hole being located on the base, and the second insertion hole being located on the bottom plate; the bottom plate is detachably connected to the base.

[0021] In one specific embodiment, the diameter of the second insertion hole is smaller than the diameter of the first insertion hole;

[0022] And / or, the first insertion port includes a through hole, and the second insertion port includes a through hole or a non-through hole.

[0023] In one specific embodiment, the magnet and the adjustment component are either detachably connected or integrated.

[0024] This application has at least the following beneficial effects:

[0025] This application relates to the field of bioseparation, specifically providing a magnetic frame that conforms to the shape of the bottom of an EP tube. The frame includes: a support with a first insertion hole and a second insertion hole spaced apart along its height, the first and second insertion holes corresponding and engaging for placing the EP tube; a magnet located between the first and second insertion holes and arranged around the bottom periphery of the EP tube; and an adjustment component connected to the magnet for adjusting the contact between the magnet and the sidewall of the EP tube. The ratio of the contact area between the magnet and the EP tube to the inner wall area of ​​the magnet is between 90% and 100%. This application, through the cooperation of the support, the magnet, and the adjustment component, allows the magnet to flexibly adjust the contact pressure with the sidewall of the EP tube, adapting to different specifications of EP tubes and enhancing the equipment's versatility. Furthermore, the adjustment component adjusts the position between the magnet and the EP tube, achieving a contact area ratio of 90%-100%, significantly enhancing the magnetic field's range of action, allowing magnetic materials (such as magnetic beads) to rapidly accumulate at the bottom of the EP tube, reducing residue and improving separation efficiency. Attached Figure Description

[0026] To more clearly illustrate the technical solutions in the embodiments of this application, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the accompanying drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0027] Figure 1 This is a schematic diagram of the overall magnetic frame that can match the shape of the bottom of the EP tube;

[0028] Figure 2 An exploded view of the magnetic frame that matches the shape of the bottom of the EP tube;

[0029] Figure 3 A schematic diagram of the magnet in the magnetic frame, which can match the shape of the bottom of the EP tube, before its position is adjusted;

[0030] Figure 4 for Figure 3 A partial sectional view of line A-A';

[0031] Figure 5 This is a schematic diagram of the magnet in the adjusted position of the magnetic frame that can match the shape of the bottom of the EP tube;

[0032] Figure 6 for Figure 5 A partial sectional view of section B-B';

[0033] Figure 7 An exploded view of a magnetic frame that matches the shape of the bottom of an EP tube.

[0034] Figure label:

[0035] 1-Support; 2-Magnet; 3-Adjustment assembly; 4-EP tube; 5-Threaded structure;

[0036] 11-First insertion hole; 12-Second insertion hole; 13-Base; 14-Base plate; 15-Fastener;

[0037] 21-Arc-shaped inner wall;

[0038] 31-Lifting assembly; 32-Elastic element;

[0039] 311-Lifting rod; 312-Locking component;

[0040] 3111 - First end; 3112 - Second end;

[0041] 41 - Upper part; 42 - Bottom part. Detailed Implementation

[0042] Various embodiments of this application will be described more fully below. This application may have various embodiments, and adjustments and changes may be made therein. However, it should be understood that there is no intention to limit the various embodiments of this application to the specific embodiments disclosed herein, but rather this application should be understood to cover all adjustments, equivalents, and / or alternatives falling within the spirit and scope of the various embodiments of this application.

[0043] In the following, the terms “comprising” or “may include” as used in the various embodiments of this application indicate the presence of the disclosed functions, operations, or elements, and do not limit the addition of one or more functions, operations, or elements. Furthermore, as used in the various embodiments of this application, the terms “comprising,” “having,” and their cognates are intended only to indicate a particular feature, number, step, operation, element, component, or combination of the foregoing, and should not be construed as primarily excluding the presence of one or more other features, numbers, steps, operations, elements, components, or combinations of the foregoing, or the possibility of adding one or more combinations of features, numbers, steps, operations, elements, components, or combinations of the foregoing.

[0044] In various embodiments of this application, the expression "or" or "at least one of A and / or B" includes any combination or all combinations of the words listed simultaneously. For example, the expression "A or B" or "at least one of A and / or B" may include A, may include B, or may include both A and B.

[0045] The terms used in the various embodiments of this application (such as "first," "second," etc.) may modify various constituent elements in the various embodiments, but do not limit the corresponding constituent elements. For example, the above terms do not limit the order and / or importance of the elements. The above terms are only used for the purpose of distinguishing one element from other elements. For example, a first user device and a second user device refer to different user devices, although both are user devices. For example, without departing from the scope of the various embodiments of this application, a first element may be referred to as a second element, and similarly, a second element may be referred to as a first element.

[0046] like Figure 1-6 As shown, this application relates to the field of biological separation, specifically providing a magnetic frame that can conform to the shape of the bottom 42 of the EP tube 4, including:

[0047] The bracket 1 has a first insertion hole 11 and a second insertion hole 12 spaced apart along its height direction. The first insertion hole 11 and the second insertion hole 12 correspond to and cooperate with each other to place the EP tube 4.

[0048] Magnet 2 is located between the first insertion hole 11 and the second insertion hole 12, and is arranged around the outer periphery of the bottom 42 of the EP tube 4;

[0049] Adjustment component 3, connected to magnet 2, is used to adjust the contact between magnet 2 and the side wall of EP tube 4;

[0050] The ratio between the contact area of ​​magnet 2 and EP tube 4 and the inner wall area of ​​magnet 2 is between 90% and 100%.

[0051] This application utilizes the cooperation between the support 1, the magnet 2, and the adjustment component 3 to allow the magnet 2 to flexibly adjust the contact pressure with the side wall of the EP tube 4, thus adapting to different specifications of EP tubes 4 and enhancing the versatility of the equipment. Furthermore, the adjustment component 3 adjusts the position between the magnet 2 and the EP tube 4, so that the contact area ratio reaches 90%-100%, greatly enhancing the range of magnetic field action, allowing magnetic materials (such as magnetic beads) to quickly accumulate at the bottom 42 of the EP tube 4, reducing residues and improving separation efficiency.

[0052] In this application, the wall of the first insertion hole 11 contacts the upper part 41 of the EP tube 4, and the wall of the second insertion hole 12 contacts the bottom 42 of the EP tube 4. The first insertion hole 11 and the second insertion hole 12 cooperate to fix the position of the EP tube 4.

[0053] like Figure 1-6 As shown, the line connecting the center of the first insertion hole 11 and the center of the second insertion hole 12 is a first straight line L1, which coincides with the axis L2 of the EP tube 4; the inner wall of the magnet 2 includes an arc-shaped inner wall 21, and the center O of the arc-shaped inner wall 21 is on the first straight line.

[0054] This application achieves a symmetrical, enveloping contact between the magnet 2 and the outer periphery of the tube bottom by aligning the center of the arc-shaped inner wall 21 of the magnet 2 with the axis of the EP tube 4 (or the first straight line). This structure ensures a uniform distribution of magnetic field strength around the tube bottom, avoiding localized magnetic blind zones caused by the offset of the magnet 2 in traditional magnetic racks. This ensures that the magnetic beads are uniformly and circularly concentrated at the tube bottom, reducing the accumulation or residue of magnetic beads caused by uneven magnetic fields. Furthermore, the line connecting the centers of the first insertion hole 11 and the second insertion hole 12 (the first straight line) coincides with the axis of the EP tube 4, significantly reducing the probability of magnetic bead dispersion caused by the tilting of the EP tube 4 relative to the displacement of the magnet 2 during experimental operations, and improving the stability of repeated experiments.

[0055] like Figure 1-6 As shown, the adjustment component 3 includes a lifting component 31 and an elastic element 32. The lifting component 31 moves along the height direction of the bracket 1 to cooperate with the magnet 2 to adjust the height of the magnet 2.

[0056] One end of the elastic element 32 is connected to the lifting assembly 31, and the other end of the elastic element 32 is connected to the magnet 2, which is used to make the magnet 2 abut against the side wall of the EP tube 4 under the elastic force.

[0057] This application utilizes a design where the lifting assembly 31 moves along the height direction of the support 1, allowing for precise adjustment of the magnet 2's height to accommodate EP tubes 4 of different sizes. This avoids the problems of insufficient magnetic force due to the magnet 2 being too high or interfering with sedimentation at the bottom of the tube caused by differences in tube length in traditional fixed magnetic racks. Furthermore, the elastic element 32 connects the lifting assembly 31 and the magnet 2, utilizing the continuous force generated by elastic deformation to ensure that the magnet 2 remains tightly pressed against the side wall of the EP tube 4. This ensures full contact between the magnet 2 and the tube wall, significantly enhancing the magnetic field's range of action. This allows magnetic materials (such as magnetic beads) to rapidly accumulate at the bottom 42 of the EP tube 4, reducing residue and improving separation efficiency.

[0058] Among them, the elastic element 32 includes a spring or elastic rubber.

[0059] like Figure 1-6 As shown, the lifting assembly 31 includes a lifting rod 311 and a locking member 312. The first end 3111 of the lifting rod 311 passes through the bracket 1 and moves along the height direction of the bracket 1. The second end 3112 of the lifting rod 311 extends along the length direction of the bracket 1, and the magnet 2 is disposed at the second end 3112 of the lifting rod 311. The lifting rod 311 is used to adjust the height of the magnet 2 when moving.

[0060] The locking element 312 is movably disposed at the first end 3111 of the lifting rod 311. The locking element 312 is placed on the top of the bracket 1 and abuts against the bracket 1 to lock the position of the lifting rod 311.

[0061] This application uses a lifting rod 311, whose first end 3111 moves vertically along the height direction of the bracket 1 and whose second end 3112 extends along the length direction of the bracket 1. This allows the lifting rod 311 to adjust the position of the magnet 2 in the height direction when it moves along the height direction, thereby adapting to EP tubes 4 of different sizes. Furthermore, the locking member 312 uses a mechanical locking method that abuts against the top of the bracket 1, resulting in a simple and stable structure.

[0062] Among them, a threaded structure 5 is provided between the locking member 312 and the lifting rod 311. The locking member 312 is movably mounted on the lifting rod 311 through the threaded structure 5, thereby realizing the locking member 312 abutting against the top of the bracket 1 to lock the position of the lifting rod 311.

[0063] like Figure 7 As shown, there are multiple first insertion holes 11, which are spaced apart along the length of the bracket 1; there are multiple second insertion holes 12, which are spaced apart along the length of the bracket 1, and the multiple second insertion holes 12 correspond one-to-one with the multiple first insertion holes 11 and cooperate to place multiple EP tubes 4.

[0064] This application utilizes multiple first insertion holes 11 and second insertion holes 12 spaced apart along the length of the support 1 to simultaneously accommodate multiple EP tubes 4, enabling parallel magnetic separation of batch samples and improving experimental efficiency. Furthermore, the one-to-one correspondence and coordinated design of the multiple insertion holes ensures that each EP tube 4 can independently benefit from the complete magnet 2 enclosure structure, avoiding differences in sample separation efficiency caused by uneven magnetic fields.

[0065] Magnet 2 includes claw magnets or tile magnets.

[0066] In one embodiment, such as Figure 2 As shown, magnet 2 includes claw magnets. The claw magnets have a hand-grabbing inverted triangular structure that can be adapted to EP tubes 4 with varying inner diameters, such as EP tubes 4 that are wider at the top and narrower at the bottom. Furthermore, the claw magnets can enhance the fit between the magnets and the outer periphery of the tube bottom, significantly increasing the range of magnetic field action, allowing magnetic materials (such as magnetic beads) to accumulate rapidly at the bottom of the tube, reducing residue.

[0067] In another embodiment, the magnet 2 includes a tile-shaped magnet (not shown in the figure), which is completely attached to the outer periphery of the tube bottom through the arc-shaped inner wall 21, greatly enhancing the range of magnetic field action, so that magnetic materials (such as magnetic beads) are rapidly enriched at the bottom of the tube and the residue is reduced.

[0068] like Figure 7As shown, the bracket 1 includes a base 13 and a bottom plate 14. The bottom plate 14 is disposed at the bottom 42 of the base 13. The first insertion hole 11 is located in the base 13, and the second insertion hole 12 is located in the bottom plate 14. The bottom plate 14 and the base 13 are detachably connected. Specifically, the bottom plate 14 and the base 13 are detachably connected by fasteners 15, including screws, bolts, pins, etc. Alternatively, one of the bottom plate 14 and the base 13 has a slot, and the other has a locking block. The slot and the locking block cooperate to achieve a locking connection.

[0069] This application allows for the adaptation of different specifications of EP tubes 4 by replacing the base plate 14 through the independent design of the base 13 and the base plate 14. The base 13 maintains the standardization of the shape and position of the first insertion hole 11, and the base plate 14 can be customized to adapt to the second insertion hole 12 of 0.5mL, 1.5mL or 2.0mL EP tubes 4. Users only need to replace the base plate 14 to switch tube types without replacing the entire magnetic frame.

[0070] like Figure 7 As shown, the diameter of the second insertion hole 12 is smaller than that of the first insertion hole 11, so that the bottom 42 is automatically limited when the EP tube 4 is inserted. The small-diameter second insertion hole 12 can accurately wrap the bottom 42 of the EP tube 4, eliminating the problem of poor contact of the magnet 2 caused by the EP tube 4 being inserted too deeply in the traditional magnetic frame. The large-diameter first insertion hole 11 allows the upper part 41 of the EP tube 4 to pass freely, avoiding excessive insertion resistance.

[0071] The first insertion port 11 includes a through hole, and the second insertion port 12 includes a through hole or a non-through hole.

[0072] In one embodiment, the first insertion port 11 includes a through hole, and the second insertion port 12 includes a through hole. The first insertion port 11 and the second insertion port 12 can be used to place tapered tubes with different upper and lower inner diameters.

[0073] In another embodiment, the first insertion port 11 includes a through hole, and the second insertion port 12 includes a non-through hole. The first insertion port 11 and the second insertion port 12 can be used to accommodate tapered tubes with different upper and lower inner diameters or round tubes with the same upper and lower inner diameters.

[0074] The magnet 2 and the adjustment component 3 are either detachably connected or integrated.

[0075] In one embodiment, the magnet 2 and the adjustment component 3 are integrated, which makes the structure more stable and eliminates the positional displacement of the magnet 2 caused by loose connection.

[0076] In another embodiment, the magnet 2 and the adjustment assembly 3 are detachably connected, allowing the user to quickly replace different types of magnets 2 to adapt to different experimental needs. Specifically, the detachable connection includes snap-fit, threaded, or magnetic connections.

[0077] It should be noted that, in this application, unless otherwise explicitly specified and defined, terms such as "installation," "connection," and "fixation" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this application according to the specific circumstances.

[0078] In this application, those skilled in the art should understand that the terms indicating orientation or positional relationship are based on the orientation or positional relationship shown in the accompanying drawings and are only for the purpose of describing this application and simplifying the description, and are not intended to indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this application.

[0079] The terminology used in the various embodiments of this application is for the purpose of describing particular embodiments only and is not intended to limit the various embodiments of this application. As used herein, the singular form is intended to include the plural form as well, unless the context clearly indicates otherwise. Unless otherwise defined, all terms used herein (including technical and scientific terms) have the same meaning as commonly understood by one of ordinary skill in the art to which the various embodiments of this application pertain. The terms (such as those defined in a generally used dictionary) are to be interpreted as having the same meaning as in the context of the relevant technical field and are not to be interpreted as having an idealized or overly formal meaning, unless clearly defined in the various embodiments of this application.

[0080] Those skilled in the art will understand that the accompanying drawings are merely schematic diagrams of a preferred embodiment, and the modules or processes shown in the drawings are not necessarily essential for implementing this application.

[0081] Those skilled in the art will understand that the modules in the apparatus of the implementation scenario can be distributed within the apparatus of the implementation scenario as described, or they can be located in one or more apparatuses different from this implementation scenario, with corresponding changes. The modules of the above-described implementation scenario can be combined into one module, or they can be further divided into multiple sub-modules.

[0082] The serial numbers in this application are for descriptive purposes only and do not represent the superiority or inferiority of the implementation scenario.

[0083] The above description is only a preferred embodiment of this application and is not intended to limit this application. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this application should be included within the protection scope of this application.

Claims

1. A magnetic frame that can conform to the shape of the bottom of an EP tube, characterized in that, include: The bracket has a first insertion hole and a second insertion hole spaced apart along its height direction. The first insertion hole and the second insertion hole correspond to and cooperate with each other for placing the EP tube. A magnet is located between the first insertion hole and the second insertion hole, and is arranged around the bottom outer periphery of the EP tube; An adjustment component, connected to the magnet, is used to adjust the contact between the magnet and the sidewall of the EP tube; The ratio between the contact area between the magnet and the EP tube and the inner wall area of ​​the magnet is between 90% and 100%.

2. The magnetic frame according to claim 1, which can conform to the shape of the bottom of the EP tube, is characterized in that, The line connecting the center of the first insertion hole and the center of the second insertion hole is a first straight line, which coincides with the axis of the EP tube; the inner wall of the magnet includes an arc-shaped inner wall, the center of which lies on the first straight line.

3. The magnetic frame according to claim 1, which can conform to the shape of the bottom of the EP tube, is characterized in that, The adjustment assembly includes a lifting assembly and an elastic element. The lifting assembly moves along the height direction of the bracket and is used to cooperate with the magnet to adjust the height of the magnet. One end of the elastic element is connected to the lifting assembly, and the other end of the elastic element is connected to the magnet, which is used to make the magnet abut against the side wall of the EP tube under the elastic force.

4. The magnetic frame according to claim 3, which can conform to the shape of the bottom of the EP tube, is characterized in that, The lifting assembly includes a lifting rod and a locking component. The first end of the lifting rod passes through the bracket and moves along the height direction of the bracket; the second end of the lifting rod extends along the length direction of the bracket, and the magnet is disposed at the second end of the lifting rod; the lifting rod is used to adjust the height of the magnet when moving. The locking member is movably disposed at the first end of the lifting rod, and the locking member is placed on the top of the bracket and abuts against the bracket to lock the position of the lifting rod.

5. The magnetic frame according to claim 4, which can conform to the shape of the bottom of the EP tube, is characterized in that, A threaded structure is provided between the locking member and the lifting rod, and the locking member is movably mounted on the lifting rod through the threaded structure.

6. The magnetic frame according to claim 1, which can conform to the shape of the bottom of the EP tube, is characterized in that, The first insertion port has multiple first insertion ports, which are spaced apart along the length direction of the bracket; The second insertion port has multiple second insertion ports, which are spaced apart along the length of the bracket. The multiple second insertion ports correspond one-to-one with the multiple first insertion ports and cooperate to place multiple EP tubes.

7. The magnetic frame according to claim 1, which can conform to the shape of the bottom of the EP tube, is characterized in that, The magnet includes claw magnets or tile magnets.

8. The magnetic frame according to claim 1, which can conform to the shape of the bottom of the EP tube, is characterized in that, The bracket includes a base and a bottom plate. The bottom plate is disposed at the bottom of the base. The first insertion hole is located on the base, and the second insertion hole is located on the bottom plate. The bottom plate is detachably connected to the base.

9. The magnetic frame according to claim 1, which can conform to the shape of the bottom of the EP tube, is characterized in that, The diameter of the second insertion port is smaller than the diameter of the first insertion port; And / or, the first insertion port includes a through hole, and the second insertion port includes a through hole or a non-through hole.

10. The magnetic frame according to claim 1, which can conform to the shape of the bottom of the EP tube, is characterized in that, The magnet and the adjustment component are either detachably connected or integrated.

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