A multi-channel nucleic acid purification device

Abstract

The utility model discloses a multi -channel nucleic acid purification device, including work table, the top of work table is provided with the storage shell, the inside of storage shell is provided with purification connection subassembly, purification connection subassembly is used for purifying nucleic acid, be provided with drive clamping assembly on purification connection subassembly, drive clamping assembly is used for the structure on purification connection subassembly is clamped fixedly. The utility model discloses through the setting up several independent filter purification cylinder, realizes the synchronous purification of multiple samples, thoroughly solved the traditional single -channel device one by one processing, the defect of low efficiency, realized the effect of multi -channel nucleic acid purification, and drive clamping assembly drives screw rod through servo motor, drives adjusting block, moving plate and the rubber clamp block synchronous action in left side, can complete all filter purification cylinder's clamping fixedly one time, need not manual operation one by one, improved the clamping efficiency, guaranteed the evenness of fixed strength simultaneously.

Description

Technical Field

[0001] This utility model relates to the field of nucleic acid purification technology, specifically a multi-channel nucleic acid purification device. Background Technology

[0002] Nucleic acid purification is a core step in molecular biology experiments and is widely used in fields such as clinical diagnosis, gene sequencing, and food safety testing.

[0003] According to application number CN202321241259.6, a nucleic acid purification device is disclosed, relating to the field of nucleic acid purification technology. The device includes a centrifuge with a turntable at its upper end and a fixed cylinder at the middle of the upper end of the turntable. The fixed cylinder has a placement cavity for holding a nucleic acid purification filter tube and a waste liquid tube. The purification filter tube is inserted into the waste liquid tube. The opening of the purification filter tube has a flange, and the outer diameter of the flange is larger than the inner diameter of the waste liquid tube. The bottom of the purification filter tube has several filter holes, and the inner bottom of the purification filter tube has a filter layer covering all the filter holes. A sealing cap is connected to the outer side of the flange of the purification filter tube via a soft plastic strip.

[0004] The nucleic acid purification devices in the above cases can only rely on a combination of a single filter purification tube and a waste liquid tube, and can only perform independent purification operations on a single sample. Single-channel devices need to complete the sample addition, purification, and collection processes one by one. When dealing with multiple samples, the operation cycle increases linearly and is time-consuming, which cannot meet the timeliness requirements of high-throughput detection. Some multi-channel related devices adopt a decentralized clamping structure, which requires manual fixing of each filter purification tube. The operation is cumbersome and prone to uneven fixing force. Therefore, we provide a multi-channel nucleic acid purification device. Utility Model Content

[0005] The purpose of this invention is to provide a multi-channel nucleic acid purification device to solve the problems mentioned in the background art.

[0006] To achieve the above objectives, the present invention provides the following technical solution: a multi-channel nucleic acid purification device, including a workbench, a storage shell on the top of the workbench, a purification connection component inside the storage shell, the purification connection component being used to purify nucleic acids, and a drive clamping component being provided on the purification connection component, the drive clamping component being used to clamp and fix the structure on the purification connection component.

[0007] Optionally, the purification connection assembly includes a partition and a filter purification tube. The partition is installed on the inner wall of the storage shell, and the number of filter purification tubes is several and they are respectively arranged above the storage shell. The bottom end of the filter purification tube passes through the storage shell and the partition from top to bottom and extends to the outside of the partition.

[0008] Optionally, a filter membrane is installed on the inner wall of the filter purification cylinder, and a number of filter holes are opened at the bottom of the filter purification cylinder. A splash-proof fixing frame for use with the filter purification cylinder is installed at the bottom of the partition and at the position corresponding to the filter purification cylinder.

[0009] Optionally, the drive clamping assembly includes a fixed housing, which is mounted on the rear side of the top of the storage housing. A servo motor is mounted on the right side of the fixed housing, and the left end of the output shaft of the servo motor passes through the fixed housing and extends into it to mount a threaded rod.

[0010] Optionally, the threaded rod has a threaded block connected to its surface, and an adjusting block is installed at the bottom of the threaded block. The bottom of the adjusting block penetrates the storage shell and extends into it. Rubber clamps are provided on the top of the partition and on both the left and right sides of the filtration and purification cylinder.

[0011] Optionally, an adjustment plate is provided on the front side of the top of the partition, and three movable plates are installed on the back of the adjustment plate. The movable plates are fixedly connected to three rubber clamps located on the left side, and the top of the movable plate located in the middle position is fixedly connected to the bottom of the adjustment block.

[0012] Optionally, a rotary motor is installed in the groove at the top of the workbench, the top end of the output shaft of the rotary motor is fixedly connected to the bottom of the storage shell, an annular groove is provided at the top of the workbench, and two annular blocks that rotate in contact with the annular groove are installed at the bottom of the storage shell inside the annular groove.

[0013] Compared with the prior art, the beneficial effects of this utility model are as follows: This invention achieves simultaneous purification of multiple samples by setting up several independent filtration and purification cylinders, completely solving the shortcomings of traditional single-channel devices that process one sample at a time and have low efficiency. It achieves the effect of multi-channel nucleic acid purification. The drive clamping component drives the threaded rod through a servo motor, which drives the adjusting block, the moving plate and the rubber clamping block on the left side to move synchronously. It can clamp and fix all filtration and purification cylinders at one time without manual operation, which improves clamping efficiency and ensures uniformity of fixing force. Attached Figure Description

[0014] Figure 1 This is a three-dimensional structural diagram of the present invention; Figure 2 This is a three-dimensional structural diagram of the workbench and storage shell of this utility model separated. Figure 3 This is a three-dimensional structural cross-sectional view of the side view of this utility model; Figure 4This is a three-dimensional structural cross-sectional view of the side view of the purification connection component and the drive clamping component of this utility model; Figure 5 This is a three-dimensional structural schematic diagram of the filter purification cylinder and filter holes of this utility model from a top view.

[0015] In the diagram: 1. Workbench; 100. Storage shell; 2. Purification connection assembly; 21. Partition; 22. Filter purification cylinder; 23. Filter membrane; 24. Filter hole; 25. Anti-splash fixing frame; 3. Drive clamping assembly; 31. Fixing shell; 32. Servo motor; 33. Threaded rod; 34. Threaded block; 35. Adjusting block; 36. Rubber clamping block; 300. Adjusting plate; 37. Moving plate; 4. Rotary motor; 5. Annular groove; 6. Annular block. Detailed Implementation

[0016] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0017] Please see Figure 1-5 A multi-channel nucleic acid purification device includes a workbench 1, a storage shell 100 on the top of the workbench 1, and the bottom of the storage shell 100 in rotatable contact with the top of the workbench 1. A controller is installed on the front of the workbench 1, and the controller is electrically connected to a rotary motor 4 and a servo motor 32. The rotary motor 4 is installed in a groove on the top of the workbench 1, and the top end of the output shaft of the rotary motor 4 is fixedly connected to the bottom of the storage shell 100. An annular groove 5 is formed on the top of the workbench 1, and two annular blocks 6 are installed on the bottom of the storage shell 100 inside the annular groove 5, which are in rotatable contact with the annular groove 5. By setting the annular groove 5 and the annular blocks 6, the stability of the storage shell 100 during rotation is improved. The rotary motor 4 drives the storage shell 100 to rotate, thereby generating centrifugal force. A discharge pipe connected to the right side of the storage shell 100 is installed, and a valve is installed on the discharge pipe.

[0018] The storage shell 100 contains a purification connection assembly 2, which includes a partition 21 and a filtration purification cartridge 22. The partition 21 is installed on the inner wall of the storage shell 100. Several filtration purification cartridges 22 are arranged above the storage shell 100. The top of each filtration purification cartridge 22 is threaded with a sealing cap. The filtration purification cartridge 22 carries the sample to be purified and, together with the filter membrane 23, separates nucleic acids from impurities. It is the core carrier for multi-channel purification. The filtration purification cartridge 22 is made of transparent polypropylene. The bottom of the filtration purification cartridge 22 extends from top to bottom through the storage shell 100 and the partition 21 and extends to the outside of the partition 21. A filter membrane 23, made of glass fiber membrane, is installed on the inner wall of the filtration purification cartridge 22. The bottom of the filter cartridge has several filter holes 24, which serve as the outflow channels for the filtered waste liquid, allowing the waste liquid separated by the filter membrane 23 to be quickly discharged into the bottom of the inner wall of the storage shell 100. At the bottom of the partition 21 and at the position corresponding to the filter purification cartridge 22, an anti-splash fixing frame 25 is installed to cooperate with the filter purification cartridge 22. The anti-splash fixing frame 25 is welded to the bottom of the partition 21 and covers the bottom of the filter purification cartridge 22 to prevent waste liquid from splashing during centrifugation. The anti-splash fixing frame 25 and the filter purification cartridge 22 are precisely matched to effectively prevent liquid splashing or waste liquid leakage during purification and avoid cross-contamination between channels. At the same time, each filter purification cartridge 22 is independently equipped with a filter membrane 23 and filter holes 24, and the sample and waste liquid are isolated throughout the process, thus blocking the contamination path from the structure.

[0019] A drive clamping assembly 3 is provided on the purification connection assembly 2. The drive clamping assembly 3 includes a fixed shell 31, which is installed on the rear side of the top of the storage shell 100. A servo motor 32 is installed on the right side of the fixed shell 31. The servo motor 32 is a forward and reverse motor with a self-locking function. The left end of the output shaft of the servo motor 32 passes through the fixed shell 31 and extends into it, where a threaded rod 33 is installed. A threaded block 34 is threadedly connected to the surface of the threaded rod 33. The surface of the threaded block 34 slides in contact with the inner wall of the fixed shell 31, which plays a guiding role, so that the force of the rotation of the threaded rod 33 can be converted into a force that drives the threaded block 34 to move left and right. An adjusting block 35 is installed at the bottom of the threaded block 34. The bottom of the adjusting block 35 passes through the storage shell 100 and extends into it, at the top of the partition 21 and located on the left and right sides of the filtration purification cylinder 22. Rubber clamps 36 are provided on both sides. The rubber clamp 36 on the left side slides in contact with the top of the partition 21, and the rubber clamp 36 on the right side is fixedly connected to the top of the partition 21. The side of the rubber clamp 36 closest to the filter purification cylinder 22 is in close contact with the filter purification cylinder 22. An adjusting plate 300 is provided on the front side of the top of the partition 21. The bottom of the adjusting plate 300 slides in contact with the top of the partition 21. Three movable plates 37 are installed on the back of the adjusting plate 300. The bottom of the movable plates 37 slides in contact with the top of the partition 21. The movable plates 37 are fixedly connected to the adjusting plate 300 by welding. The movable plates 37 are fixedly connected to the three rubber clamps 36 on the left side. The top of the movable plate 37 in the middle position is fixedly connected to the bottom of the adjusting block 35. The adjusting block 35 is fixedly connected to the movable plate 37 by welding.

[0020] In use, add the sample to be purified, such as 200 μL of lysed blood sample, to each filtration purification cartridge 22 in sequence to ensure that the sample volume is consistent. Then add the corresponding dose of binding solution, close the sealing cap, and start the servo motor 32 through the controller. The servo motor 32 rotates forward, driving the threaded rod 33 to rotate. The threaded block 34 slides to the right along the inner wall of the fixed shell 31, and then pushes the moving plate 37 in the middle position to the right through the adjusting block 35. The moving plate 37 drives the adjusting plate 300 and all the left rubber clamps 36 to move to the right synchronously, and cooperates with the right fixed rubber clamps 36 to clamp all the filtration purification cartridges 22 at one time. Start the rotary motor 4 to drive the storage shell 100 and the internal purification connection component 2 and the clamping component 3 to rotate synchronously. The annular block 6 slides smoothly along the annular groove 5 to ensure rotational concentricity and reduce vibration. Under the action of centrifugal force, impurities such as proteins and cell debris in the sample are intercepted by the filter membrane 23. The waste liquid flows into the waste liquid collection area at the bottom of the storage shell 100 through the filter hole 24. The anti-splash fixing frame 25 effectively blocks the splashing of waste liquid and avoids cross-contamination between channels. After centrifugation is completed, the rotary motor 4 stops. Turn off the controller, release the drive clamping assembly 3, open the sealing cap of each filtration purification cartridge 22, add washing solution simultaneously, tighten the sealing cap, restart the clamping function to fix the filtration purification cartridge 22, start the rotary motor 4, centrifuge at the same speed, the washing waste liquid is discharged through the filter hole 24, repeat washing twice to thoroughly remove the impurities remaining on the filter membrane 23, release the clamp again, open the sealing cap, add elution solution to each filtration purification cartridge 22, tighten the sealing cap, fix the filtration purification cartridge 22, restart the rotary motor 4, centrifuge at a lower speed to promote the full elution of nucleic acid from the filter membrane 23, then stop the rotation to allow the elution solution to fully contact the filter membrane 23 to ensure the nucleic acid recovery rate, release the drive clamping assembly 3, remove all filtration purification cartridges 22, open the sealing cap, and use a pipette to remove the elution solution from the cartridge.

[0021] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A multi-channel nucleic acid purification device, characterized in that: The device includes a workbench (1), a storage shell (100) is provided on the top of the workbench (1), a purification connection component (2) is provided inside the storage shell (100), the purification connection component (2) is used to purify nucleic acids, and a drive clamping component (3) is provided on the purification connection component (2), the drive clamping component (3) is used to clamp and fix the structure on the purification connection component (2).

2. The multi-channel nucleic acid purification device according to claim 1, characterized in that: The purification connection assembly (2) includes a partition (21) and a filtration purification tube (22). The partition (21) is installed on the inner wall of the storage shell (100). There are several filtration purification tubes (22) and they are respectively arranged above the storage shell (100). The bottom end of the filtration purification tube (22) passes through the storage shell (100) and the partition (21) from top to bottom and extends to the outside of the partition (21).

3. The multi-channel nucleic acid purification device according to claim 2, characterized in that: A filter membrane (23) is installed on the inner wall of the filter purification cylinder (22). Several filter holes (24) are opened at the bottom of the filter purification cylinder (22). A splash-proof fixing frame (25) is installed at the bottom of the partition (21) and at the position corresponding to the filter purification cylinder (22) to cooperate with the filter purification cylinder (22).

4. The multi-channel nucleic acid purification device according to claim 2, characterized in that: The drive clamping assembly (3) includes a fixed housing (31) which is mounted on the rear side of the top of the storage housing (100). A servo motor (32) is mounted on the right side of the fixed housing (31). The left end of the output shaft of the servo motor (32) passes through the fixed housing (31) and extends into it to install a threaded rod (33).

5. The multi-channel nucleic acid purification device according to claim 4, characterized in that: The threaded rod (33) has a threaded block (34) threadedly connected to its surface. An adjusting block (35) is installed at the bottom of the threaded block (34). The bottom of the adjusting block (35) penetrates the storage shell (100) and extends into its interior. Rubber clamps (36) are provided on the top of the partition (21) and on both the left and right sides of the filtration and purification cylinder (22).

6. The multi-channel nucleic acid purification device according to claim 5, characterized in that: An adjustment plate (300) is provided on the front side of the top of the partition (21). Three movable plates (37) are installed on the back of the adjustment plate (300). The movable plates (37) are fixedly connected to three rubber clamps (36) located on the left side. The top of the movable plate (37) located in the middle position is fixedly connected to the bottom of the adjustment block (35).

7. The multi-channel nucleic acid purification device according to claim 1, characterized in that: A rotary motor (4) is installed in the groove at the top of the workbench (1). The top end of the output shaft of the rotary motor (4) is fixedly connected to the bottom of the storage shell (100). An annular groove (5) is provided at the top of the workbench (1). Two annular blocks (6) that rotate in contact with the annular groove (5) are installed at the bottom of the storage shell (100) and inside the annular groove (5).

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