Diatom culture device

Abstract

The utility model discloses a diatom culture device, including outer vessel and inner vessel, the outer vessel is equipped with the base, the inner vessel is nested in the inside of outer vessel, the inside of inner vessel inserts and combines with the adsorption board, the top of adsorption board is combined with annular flow guide groove, the outer wall of inner vessel is wound with flexible heating band, the utility model discloses the hollow cavity of annular flow guide groove and the flow guide hole of equidistance distribution of outer wall form the cooperative structure, and the mild liquid supplementing channel is constructed, and the liquid inlet of annular flow guide groove top can steady and receive fresh culture medium, and the inside hollow cavity plays the role of buffer pressure stabilization, and the flow guide hole of outer wall is closely combined with the inner wall of inner vessel, makes the culture medium along the inner wall of inner vessel slowly and evenly after the flow guide hole, avoids the influence of water flow impact to algal body when supplementing liquid, prevents diatom adhered to adsorption board from being washed off, dispersing or causing mechanical damage, provides the sustained and stable growth condition for diatom.

Description

Technical Field

[0001] This utility model mainly relates to the field of diatom cultivation technology, specifically a diatom cultivation device. Background Technology

[0002] Diatom cultivation refers to the process of inoculating, proliferating, multiplying, or selectively cultivating diatoms under artificially controlled environmental conditions, taking into account their physiological characteristics and growth requirements. This is achieved by selecting high-quality algal strains, preparing suitable culture media, and controlling key factors such as light, temperature, pH, and dissolved oxygen. Its core objectives include providing natural feed for aquaculture, providing highly active algal strains for the field of bioengineering, providing standardized research materials for scientific research experiments, or purifying water quality through the ecological functions of diatoms.

[0003] Diatom cultivation devices are specialized equipment designed and manufactured based on the technical principles and practical needs of diatom cultivation. They are used to support diatom growth and achieve precise control of cultivation conditions, creating a stable and controllable artificial environment for diatom growth. They can meet the diatom cultivation needs in various scenarios, including algal purification, small-batch propagation, and large-scale cultivation. Traditional diatom cultivation devices often suffer from water flow impact during culture medium replacement or replenishment due to direct pouring, causing attached algae to detach, disperse, or suffer mechanical damage. This also easily leads to the resuspension of metabolic waste at the bottom, disrupting the stability of the cultivation system, and resulting in a high algal loss rate during medium changes. Utility Model Content

[0004] This utility model addresses the problem of overly simplistic existing solutions by providing a diatom cultivation device. This device solves the problems mentioned in the background section regarding traditional diatom cultivation devices, where direct pouring during culture medium replacement or replenishment easily causes water flow impact, leading to detachment, dispersion, or mechanical damage of attached algae. It also causes bottom metabolic waste to resuspend, disrupting the stability of the cultivation system, and results in a high algae loss rate during medium replacement.

[0005] The technical solution adopted by this utility model to solve the above-mentioned technical problems is as follows: A diatom cultivation device includes an outer vessel and an inner vessel, with a base located below the outer vessel and the inner vessel nested inside the outer vessel.

[0006] An adsorption plate is inserted inside the inner container, and an annular guide groove is attached to the top of the adsorption plate. A flexible heating strip is wrapped around the outer wall of the inner container.

[0007] Furthermore, the top of the outer container is provided with a top cover, the surface of which is distributed with a plurality of microporous structures for ventilation, and the two ends of the top cover are rotatably connected with handles, the side of the handles that are in contact with the outer container having an interlocking structure.

[0008] Furthermore, both the outer and inner containers are cylindrical in shape, with the bottom of the outer container being an arc-shaped structure that tapers towards the center, while the bottom of the inner container is flat. The outer container has a support platform fixed to the inner wall of the corresponding circular structure for supporting the inner container. The surface of the support platform is evenly distributed with multiple through-grid structures. An independent cavity is formed between the support platform and the arc-shaped structure below the outer container. The outer container has a waste liquid discharge port at the bottom of the corresponding arc-shaped structure.

[0009] Furthermore, there is a gap between the outer wall of the inner container and the inner wall of the outer container, and the inner wall of the outer container is covered with heat-insulating cotton at the position corresponding to the distribution of the flexible heating bands, which are distributed in a spiral shape.

[0010] Furthermore, the adsorption plate is composed of multiple single plates, each of which has a honeycomb-shaped pore structure for diatom attachment. The height of each single plate is the distance between the bottom wall of the inner container and the bottom of the annular guide groove. The outer diameter of the adsorption plate is adapted to the inner diameter of the inner container, and the position of each single plate is set according to the inner diameter distance between the inner walls of the inner container.

[0011] Furthermore, the top of the annular guide channel is provided with an inlet for mounting a spiral sealing cap. The axis of the annular guide channel is a through channel with a hollow cavity inside for containing culture medium. The outer wall of the annular guide channel is provided with multiple guide holes that communicate with the hollow cavity at equal intervals along its shape. The guide holes fit against the inner wall of the inner vessel.

[0012] Furthermore, an interface tube is provided on one side of the outer wall of the inner container, and a piston for cooperating with the interface tube is provided at a corresponding position on the outer wall of the outer container.

[0013] Compared with the prior art, the beneficial effects of this utility model are as follows: 1. This device utilizes a synergistic structure formed by the hollow cavity of the annular flow channel and the equidistantly distributed flow holes on the outer wall to create a gentle replenishment channel. The inlet at the top of the annular flow channel can stably receive fresh culture medium, while the internal hollow cavity acts as a buffer and pressure stabilizer. The flow holes on the outer wall fit tightly against the inner wall of the inner vessel, allowing the culture medium to flow slowly and evenly along the inner wall of the inner vessel after passing through the flow holes. This completely eliminates the traditional method of direct pouring for replenishment. This structure avoids the impact of water flow on the algae during replenishment from the source, preventing the diatoms attached to the adsorption plate from being washed away, dispersed, or mechanically damaged, thus providing continuous and stable growth conditions for the diatoms and ensuring the continuity of the algal growth state.

[0014] 2. The axial through-channel structure of the annular guide channel, without obstructing light or the observation field, combined with the annular equidistant layout of the guide holes, allows fresh culture medium to flow down the entire inner wall of the inner vessel, achieving a smooth replacement with the old culture medium. At the same time, the buffered flow of culture medium will not wash away the diatoms on the adsorption plate. The honeycomb pore structure of the adsorption plate not only provides a stable habitat for diatoms, but its three-dimensionally distributed pores can also guide the replenishment liquid to form a microcirculation, accelerating the replacement of fresh and old culture medium, and promoting the penetration of nutrients into the honeycomb pores. This ensures that the diatoms attached deep in the pores can also fully obtain nutrients. In addition, the structure of the adsorption plate and the inner diameter of the inner vessel are adapted so that the thin film liquid flow formed when the replenishment liquid flows down the inner wall can fully contact the surface of the adsorption plate, further improving the nutrient absorption efficiency.

[0015] The present invention will be explained in detail below with reference to the accompanying drawings and specific embodiments. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the main structure of this utility model; Figure 2 This is a schematic diagram of a partial internal structure of the present invention from the main view. Figure 3 This is a schematic diagram of the internal structure of the outer vessel of this utility model; Figure 4 This is a schematic diagram of the inner vessel structure of this utility model; Figure 5 This is a schematic diagram of the adsorption plate and annular guide groove structure of this utility model.

[0017] Numbering on the map: 1. Outer container; 11. Top cover; 12. Support platform; 13. Piston; 14. Waste liquid discharge port; 2. Inner container; 21. Adsorption plate; 22. Annular guide channel; 23. Flexible heating belt; 24. Interface pipe; 3. Base. Detailed Implementation

[0018] To facilitate understanding of this utility model, a more comprehensive description of the utility model will be given below with reference to the accompanying drawings, which show several embodiments of the utility model. However, the utility model can be implemented in different forms and is not limited to the embodiments described in the text. On the contrary, these embodiments are provided to make the disclosure of the utility model more thorough and comprehensive.

[0019] It should be noted that when an element is referred to as being "fixed to" another element, it can be directly on the other element or there may be an intervening element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or there may be an intervening element. The terms "vertical," "horizontal," "left," "right," and similar expressions used in this document are for illustrative purposes only.

[0020] Please refer to the appendix carefully. Figure 1-5 A diatom cultivation device includes an outer container 1 and an inner container 2. A base 3 is provided below the outer container 1, and the inner container 2 is nested inside the outer container 1.

[0021] An adsorption plate 21 is inserted inside the inner container 2, and an annular guide groove 22 is attached to the top of the adsorption plate 21. A flexible heating belt 23 is wrapped around the outer wall of the inner container 2.

[0022] In this embodiment, as Figure 1 , Figure 2 and Figure 3 As shown, the top of the outer container 1 is provided with a top cover 11. The surface of the top cover 11 is distributed with a plurality of microporous structures for ventilation. The two ends of the top cover 11 are rotatably connected with handles. The side of the handles that are in contact with the outer container 1 is provided with a locking structure.

[0023] With the above structure, the top of the top cover 11 also has a special pipe opening. This pipe opening can be inserted into a silicone tube and penetrate deep into the inner container 2. The other end of the tube is connected to a small air pump. The airflow intensity of the air pump can be controlled at 0.05-0.1m / s to continuously deliver sterile air to the culture system, meet the oxygen requirements for diatom growth, and promote the uniform distribution of nutrients in the culture medium through airflow disturbance. The perforated structure distributed on the surface of the top cover 11 can realize efficient air circulation inside and outside the device, effectively prevent external pollutants from entering during the culture process, and ensure the stability of the internal temperature, humidity and gas environment of the device. It is a detachable structure.

[0024] In this embodiment, as Figure 1 , Figure 2 and Figure 3 As shown, both the outer container 1 and the inner container 2 are cylindrical structures. The bottom of the outer container 1 is a circular arc structure that tapers towards the center point, while the bottom of the inner container 2 is a flat structure. The outer container 1 has a support platform 12 fixed on the inner wall of the corresponding circular structure for supporting the inner container 2. The surface of the support platform 12 is evenly distributed with multiple through-grid structures. An independent cavity is formed between the support platform 12 and the circular arc structure below the outer container 1. The outer container 1 has a waste liquid discharge port 14 at the bottom of the corresponding circular arc structure.

[0025] Through the above structure, the inner wall of the outer container 1 is equipped with a temperature sensor, which is electrically connected to the external temperature control system together with the flexible heating belt 23. The arc structure of the bottom of the outer container 1, which gradually narrows towards the center point, can guide the waste liquid to gather at the bottom. With the waste liquid discharge port 14 opened at the bottom, the waste liquid can be discharged quickly and completely. The support platform 12 provides a stable support for the inner container 2. At the same time, the surface of the support platform 12 is provided with matching insertion points at the corresponding positions of the outer wall of the inner container 2, which can effectively ensure the stability of the inner container 2 when it is placed inside. It also lays the foundation for the coaxial alignment of the interface pipe 24 and the piston 13. The through-type grid structure of the support platform 12 does not block the flow path of the waste liquid, ensuring that the waste liquid is discharged smoothly from the inner container 2.

[0026] In this embodiment, as Figure 5 As shown, there is a gap between the outer wall of the inner container 2 and the inner wall of the outer container 1, and the inner wall of the outer container 1 is covered with heat-insulating cotton at the position corresponding to the distribution of the flexible heating bands 23, which are distributed in a spiral shape.

[0027] With the above structure, the spirally distributed flexible heating band 23 closely adheres to the outer wall of the inner container 2, enabling uniform heating of the entire circumferential and axial areas of the inner container 2, providing a constant temperature environment for diatom growth. The heating band is connected to an external temperature control system, which can adjust the heating power and control the temperature within the optimal growth range of diatoms by using the real-time feedback of the cultivation environment temperature from the temperature sensor located on the inner wall of the outer container 1. The surface of the insulation cotton is covered with an aluminum foil layer, which not only effectively reduces heat loss to the external environment, lowers energy consumption, and maintains temperature stability, but also has good heat reflection properties, reflecting the radiant heat emitted by the heating band back to the inner container 2, further improving heat utilization efficiency.

[0028] In this embodiment, as Figure 5 As shown, the adsorption plate 21 is composed of multiple single plates. Each single plate has a honeycomb-shaped pore structure for diatom attachment. The height of each single plate is the distance between the bottom wall of the inner container 2 and the bottom of the annular guide groove 22. The outer diameter of the adsorption plate 21 is adapted to the inner diameter of the inner container 2. The position of each single plate is set according to the inner diameter distance between the inner walls of the inner container 2.

[0029] Through the above structure, the modular structure composed of multiple single plates, combined with the evenly distributed honeycomb pore structure, greatly expands the effective attachment area of ​​diatoms. The honeycomb pores provide stable habitat sites for diatoms and reduce the impact of water flow disturbance on the algae. The height of each single plate matches the distance from the bottom wall of the inner container 2 to the bottom of the annular guide channel 22, and the outer diameter of the adsorption plate 21 is perfectly matched with the inner diameter of the inner container 2, ensuring that the adsorption plate 21 is stably inserted into the inner container 2. With the help of the annular sealing gasket at the bottom of the top cover 11, it is attached to the top of the annular guide channel 22, ensuring that there is no shaking gap between the components, while making full use of the cultivation space. Its detachable structure also makes it easy to disassemble and clean, effectively avoiding cross-contamination caused by algal residue. At the same time, the number of single plates can be flexibly adjusted according to the cultivation scale, improving the reusability of the device.

[0030] In this embodiment, as Figure 4 and Figure 5 As shown, the top of the annular guide channel 22 is provided with an inlet for mounting a spiral sealing cap. The axis of the annular guide channel 22 is a through channel with a hollow cavity inside for containing culture medium. The outer wall of the annular guide channel 22 is provided with multiple guide holes that communicate with the hollow cavity at equal intervals along its shape. The guide holes fit into the inner wall of the inner vessel 2.

[0031] With the above structure, the through groove at the axis does not block the light and observation field inside the inner container 2, ensuring the convenience of monitoring the photosynthetic needs and growth status of diatoms. The synergy between the hollow cavity inside and the guide holes on the outer wall edge enables water to be replenished along the wall instead of being poured directly. This can effectively avoid the formation of impact water flow when fresh culture medium is injected, preventing the diatoms cultured in the inner container 2 from being washed away, dispersed, or mechanically damaged by the water flow.

[0032] In this embodiment, as Figure 2 and Figure 3 As shown, an interface tube 24 is provided on one side of the outer wall of the inner container 2, and a piston 13 is provided at the corresponding position on the outer wall of the outer container 1 for use in conjunction with the interface tube 24.

[0033] With the above structure, the piston 13 is made of food-grade polytetrafluoroethylene and is conical in shape. One side of the conical structure is connected to an external handle by a rod. The outer container 1 has a guide hole for the rod of the piston 13 to slide at the corresponding position. At the same time, the outer wall of the outer container 1 has an insertion groove that matches the handle at the corresponding position. When the piston 13 is pushed in along the guide hole until it is completely sealed with the interface tube 24, the handle can be inserted and fixed with the insertion groove on the outer wall of the outer container 1. Compared with the planar structure, the piston 13 can not only be aligned by the conical guide during insertion and removal, but also enhance the sealing effect by gradually increasing the conical fit during the pushing process, effectively preventing leakage of culture medium or waste liquid.

[0034] The specific operating procedure of this utility is as follows: Both the outer container 1 and the inner container 2 of this diatom cultivation device are made of transparent high borosilicate glass, which has good light transmittance and can be used in conjunction with lighting equipment such as LED plant growth lights to provide sufficient and suitable light source for diatom photosynthesis.

[0035] When in use, first assemble the adsorption plate 21 inside the inner container 2, and pour in the culture medium mixed with diatom seeds. In daily cultivation, combined with the temperature control function of the external temperature control system for the flexible heating belt 23 and the light supply of the external LED plant growth lamp, the suitable growth environment of diatoms can be simulated to promote their proliferation.

[0036] The top cover 11 has a pre-drilled opening for inserting a silicone tube into the inner container 2. The other end of the tube is connected to a small air pump to continuously provide sterile air to the culture system, which not only meets the oxygen requirements for diatom growth, but also mixes the culture medium evenly through airflow disturbance, preventing algae from settling.

[0037] The flexible heating band 23 and the temperature sensor set on the inner wall of the outer container 1 are connected to the external temperature control system, which can monitor and adjust the temperature of the cultivation environment in real time to ensure that it is stable in the optimal range for diatom growth. The multiple breathable micropores distributed on the surface of the top cover 11 can realize the exchange of internal and external gases, provide carbon dioxide for the growth of diatoms, maintain the internal air pressure balance as much as possible, and avoid contamination of the internal cultivation environment.

[0038] During diatom cultivation, the transparency of the outer vessel 1 and inner vessel 2 allows for direct observation of whether the internal culture medium is turbid, thus determining the growth status of the diatoms. When the culture medium needs to be replaced, the handles on both sides of the top cover 11 are used to remove the top cover 11 by pushing the handles outwards. At the same time, the annular guide groove 22 is placed on top of the adsorption plate 21, the sealing cap of the waste liquid discharge port 14 is unscrewed, and the piston 13 is pulled at the pipe opening corresponding to the piston 13 in the outer vessel 1, so that one end of it is detached from the interface tube 24. Since the interface tube 24 is equipped with a baffle plate with a microporous structure near the inside, the culture medium containing metabolic waste in the inner vessel 2 can flow out, while the diatom body will not flow out with the waste liquid. In addition, the adsorption plate 21 can further fix the algae and prevent them from being lost.

[0039] As the waste liquid in the inner vessel 2 enters the outer vessel 1 through the interface pipe 24, it flows down through the grid structure of the support platform 12 and is finally discharged into the outer collection vessel from the waste liquid discharge port 14. At this time, if it is observed that the discharge of waste liquid inside the inner vessel 2 is nearly completed, a fresh culture medium container can be connected to the liquid inlet at the top of the annular guide channel 22, so that the fresh culture medium enters the hollow cavity of the annular guide channel 22 and is finally discharged from its extended guide hole, flowing in along the inner wall of the inner vessel 2 to replenish the culture medium.

[0040] The present invention has been described above by way of example in conjunction with the accompanying drawings. Obviously, the specific implementation of the present invention is not limited to the above-described manner. Any non-substantial improvement made by adopting the inventive concept and technical solution of the present invention, or the direct application of the inventive concept and technical solution of the present invention to other occasions without modification, shall be within the protection scope of the present invention.

Claims

1. A diatom cultivation device, characterized in that: It includes an outer vessel (1) and an inner vessel (2), with a base (3) below the outer vessel (1) and the inner vessel (2) nested inside the outer vessel (1); An adsorption plate (21) is inserted inside the inner vessel (2), and an annular guide groove (22) is attached to the top of the adsorption plate (21). A flexible heating belt (23) is wrapped around the outer wall of the inner vessel (2).

2. The diatom cultivation device according to claim 1, characterized in that: The top of the outer container (1) is provided with a top cover (11). The surface of the top cover (11) is distributed with a plurality of microporous structures for ventilation. The two ends of the top cover (11) are rotatably connected with handles. The side of the handle that is in contact with the outer container (1) is provided with a locking structure.

3. The diatom cultivation device according to claim 1, characterized in that: Both the outer vessel (1) and the inner vessel (2) are cylindrical structures. The bottom of the outer vessel (1) is a circular arc structure that tapers towards the center point, while the bottom of the inner vessel (2) is a flat structure. The outer vessel (1) has a support platform (12) fixed on the inner wall of the corresponding circular structure for supporting the inner vessel (2). The surface of the support platform (12) is evenly distributed with multiple through-grid structures. An independent cavity is formed between the support platform (12) and the circular arc structure below the outer vessel (1). The outer vessel (1) has a waste liquid discharge port (14) at the bottom of the corresponding circular arc structure.

4. The diatom cultivation device according to claim 1, characterized in that: There is a gap between the outer wall of the inner vessel (2) and the inner wall of the outer vessel (1), and the inner wall of the outer vessel (1) is covered with heat-insulating cotton at the position corresponding to the distribution of the flexible heating band (23), and the flexible heating band (23) is distributed in a spiral shape.

5. The diatom cultivation device according to claim 1, characterized in that: The adsorption plate (21) is composed of multiple single plates. Each single plate is provided with a honeycomb-shaped pore structure for diatom attachment. The height of each single plate is the distance between the bottom wall of the inner vessel (2) and the bottom of the annular guide groove (22). The outer diameter of the adsorption plate (21) is adapted to the inner diameter of the inner vessel (2). The position of each single plate is set according to the inner diameter distance between the inner walls of the inner vessel (2).

6. The diatom cultivation device according to claim 1, characterized in that: The top of the annular guide channel (22) is provided with an inlet for mounting a spiral sealing cap. The annular guide channel (22) is a through channel at its axis and has a hollow cavity inside for containing culture medium. The outer wall of the annular guide channel (22) is provided with multiple guide hole structures that are connected to the hollow cavity at equal intervals along its shape. The guide hole structures are in contact with the inner wall of the inner vessel (2).

7. The diatom cultivation device according to claim 1, characterized in that: The inner vessel (2) has an interface tube (24) on one side of its outer wall, and the outer vessel (1) has a piston (13) at a corresponding position on its outer wall for use with the interface tube (24).

Images