Multifunctional enzymolysis reaction device suitable for chlorophyll extraction

Abstract

The utility model provides a kind of multifunctional enzymatic reaction device suitable for chlorophyll extraction, it is related to biological chemical industry equipment technical field, including reaction tank main part, the both sides of reaction tank are equipped with support leg, the top center of reaction tank is equipped with motor, the side of motor is equipped with feed inlet, the bottom center of reaction tank is equipped with discharge port, the bottom of discharge port is equipped with discharge valve, the bottom of discharge valve is equipped with integrated solid-liquid separation module, adopt stirring system, realized using composite stirring system, wall scraper can continuously remove viscous material on inner wall, eliminate heat transfer dead angle, ensure the efficient heat exchange between material and heating medium, so that the temperature field in reaction tank is highly uniform, stable in the optimum activity temperature range of enzyme, this uniformity and stability, combine the sufficient macroscopic mixing effect of paddle, ensure that enzyme and substrate are contacted comprehensively and continuously, so that enzymatic reaction time is shortened, significantly improve the rupture rate of cell wall and the extraction rate of chlorophyll.

Description

Technical Field

[0001] This utility model relates to the field of biochemical equipment technology, and in particular to a multifunctional enzymatic hydrolysis reaction device suitable for chlorophyll extraction. Background Technology

[0002] According to a chlorophyll extraction process disclosed in Chinese Patent No. CN105622623B, the process includes the following steps: A. Pretreatment: Fresh corn stalks are coarsely crushed into granules using a coarse crusher; B. Hydrolysis: The coarsely crushed corn stalk granules are added to a hydrolysis device for hydrolysis; C. Filtration: After hydrolysis, the hydrolysate is obtained by filtration; D. Enzyme addition: The temperature of the hydrolysate is controlled, and ligninase and cellulase are added to the hydrolysate; E. Enzyme inactivation: The hydrolysate is heated to 80℃ to inactivate the enzymes; F. Cooling: The hydrolysate is cooled, and amylase, protease, and papain are added to the hydrolysate; G. Separation: Chlorophyll is separated from the mother liquor by controlling the centrifugation speed of a centrifuge; H. Drying: The water in the chlorophyll is evaporated by controlling the temperature and humidity of a dryer to obtain the chlorophyll product.

[0003] The aforementioned comparative documents and existing technologies have the following technical problems: When processing high-viscosity plant slurry, existing traditional reactors are prone to heat transfer dead zones and local temperature unevenness, which leads to low enzymatic hydrolysis efficiency. Moreover, after the reaction, due to the slow heating rate of the equipment, the enzyme cannot be deactivated quickly, causing chlorophyll to be exposed to active enzymes and the heat environment for a long time, making it extremely easy to degrade and deteriorate. Existing processes require the material to be transferred from the reactor to an external heater and solid-liquid separation equipment. This step-by-step operation not only prolongs the production cycle and increases energy consumption, but also brings the risk of material loss and oxidative pollution during the transfer process. Traditional equipment has low precision in temperature and pH feedback and control, making it difficult to ensure the batch stability and consistency of chlorophyll extraction products in large-scale production. Utility Model Content

[0004] The purpose of this invention is to address the shortcomings of existing technologies by proposing a multifunctional enzymatic hydrolysis reaction device suitable for chlorophyll extraction.

[0005] To achieve the above objectives, the present invention adopts the following technical solution: a multifunctional enzymatic hydrolysis reaction device suitable for chlorophyll extraction, comprising a reaction tank body, supporting legs on both sides of the reaction tank, a motor at the top center of the reaction tank, a feed inlet on one side of the motor, a discharge outlet at the bottom center of the reaction tank, a discharge valve at the bottom of the discharge outlet, and an integrated solid-liquid separation module at the bottom of the discharge valve.

[0006] Preferably, the integrated solid-liquid separation module has a residue discharge port at the bottom and a filtrate outlet on one side of the residue discharge port.

[0007] Preferably, the reaction vessel has a stirring system at its center and a measuring unit at its top.

[0008] Preferably, the stirring system includes a rotary rod, a connecting rod on one side of the rotary rod, a detachable wall scraper at the end of the connecting rod, and a paddle-type main stirrer at the bottom end of the rotary rod.

[0009] Preferably, the rotating rod passes through the top of the reaction vessel and is connected to the motor; the rotating rod is welded to the connecting rod; and the end face of the detachable wall scraper is chamfered.

[0010] Preferably, the inner wall of the reaction vessel is provided with a temperature control jacket, and the interior of the integrated solid-liquid separation module is provided with a detachable porous filter plate.

[0011] Preferably, the reaction vessel and the support leg are connected by welding, and the bottom of the support leg is provided with a pad.

[0012] Beneficial effects This invention employs a stirring system. In traditional enzymatic hydrolysis methods, the high viscosity of plant pulp can easily form a heat transfer resistance layer on the inner wall of the reactor, causing localized overheating or underheating. This device uses a composite stirring system, and the wall scraper can continuously remove sticky materials from the inner wall, eliminating heat transfer dead zones and ensuring efficient heat exchange between the material and the heating medium. This results in a highly uniform temperature field within the reactor, stabilizing it within the optimal activity temperature range of the enzyme. This uniformity and stability, combined with the thorough macroscopic mixing effect of the impeller, ensures comprehensive and continuous contact between the enzyme and the substrate, thereby shortening the enzymatic hydrolysis reaction time and significantly improving the cell wall rupture rate and chlorophyll extraction yield.

[0013] This invention employs a temperature-controlled jacket and a stirring system. Chlorophyll is extremely sensitive to heat, acid, and oxygen. After the enzymatic hydrolysis process, the residual enzymes must be rapidly inactivated; otherwise, they will hydrolyze the chlorophyll, causing a decline in quality. This device uses a double-layered, high-efficiency temperature-controlled jacket, combined with the real-time wall-scraping action of the stirrer, to achieve rapid and precise temperature rise in the reaction. The temperature is raised to the enzyme inactivation temperature in a very short time. This rapid thermal shock can immediately terminate enzyme activity, avoiding degradation of chlorophyll caused by prolonged residence at high temperatures. Ultimately, this ensures the high purity and high stability of chlorophyll in the extract, enhancing the market value of the final product.

[0014] This invention employs a reaction vessel and an integrated solid-liquid separation module. In traditional processes, enzymatic hydrolysis and solid-liquid separation are two independent steps. Materials must be transferred from the reaction vessel to a filter or centrifuge via pumps or pipelines. This not only increases the number of operation steps and extends the production cycle but also easily leads to material residue and oxidation exposure risks during the transfer process. This device directly installs a detachable microporous filter plate assembly at the bottom of the tank, enabling the three key steps of enzymatic hydrolysis, inactivation, and preliminary solid-liquid separation to be completed in one stop within the same equipment. This integrated operation significantly shortens batch production time, reduces the pumping energy consumption required for material transfer, and fundamentally eliminates the risk of loss and contamination during the material transfer process. Attached Figure Description

[0015] Figure 1 This is a cross-sectional view of the present invention; Figure 2 This is an isometric view of the present invention; Figure 3 This is a front view of the present invention; Figure 4 This is a side view of the present invention; Figure 5 This is a schematic diagram of the stirring system of this utility model; Figure 6 This is a bottom view of the stirring system of this utility model.

[0016] Legend: 1. Reaction vessel; 101. Temperature control jacket; 2. Support leg; 3. Motor; 4. Feed inlet; 5. Discharge outlet; 6. Discharge valve; 7. Integrated solid-liquid separation module; 701. Detachable porous filter plate; 8. Residue discharge port; 9. Filtrate outlet; 10. Stirring system; 1001. Rotary rod; 1002. Connecting rod; 1003. Detachable wall scraper; 1004. Paddle-type main stirrer; 11. Measuring unit. Detailed Implementation

[0017] To make the technical means, creative features, and achieved objectives and effects of this utility model easier to understand, the present utility model is further described below with reference to specific embodiments and accompanying drawings. However, the following embodiments are merely preferred embodiments of this utility model and not all of them. Other embodiments obtained by those skilled in the art based on the embodiments described in the implementation plan without creative effort are all within the protection scope of this utility model.

[0018] The specific embodiments of this utility model are described below with reference to the accompanying drawings. Specific Implementation Example 1: Reference Figure 1-6This utility model provides a multifunctional enzymatic hydrolysis reaction device suitable for chlorophyll extraction, including a reaction tank body 1, support legs 2 fixed downward on both sides of the reaction tank 1, a motor 3 fixedly installed at the top center of the reaction tank 1, a feed inlet 4 at the top of the reaction tank 1 and on one side of the motor 3, a discharge port 5 at the bottom center of the reaction tank 1, a discharge valve 6 at the bottom of the discharge port 5, an integrated solid-liquid separation module 7 detachably connected to the bottom of the discharge valve 6, a residue discharge port 8 at the bottom of the integrated solid-liquid separation module 7, a filtrate outlet 9 on one side of the residue discharge port 8, a stirring system 10 at the center inside the reaction tank 1, and a measuring unit 11 at the top inside the reaction tank 1. The measuring unit 11 is equipped with a communication interface or a wireless communication unit for communicating with the central controller. The stirring system 10 includes a vertically arranged rotary rod 1001, a connecting rod 1002 horizontally fixed to the side of the rotary rod 1001, a detachable wall scraper 1003 fixed to the end of the connecting rod 1002, and a paddle-type main stirrer 1004 fixed to the bottom of the rotary rod 1001. The rotary rod 1001 passes through the top of the reaction tank 1 and is connected to the motor 3 for transmission. The rotary rod 1001 and the connecting rod 1002 are welded together. The end face of the detachable wall scraper 1003 is chamfered. The inner wall of the reaction tank 1 is provided with a temperature control jacket 101. The integrated solid-liquid separation module 7 is provided with a detachable porous filter plate 701. The reaction tank 1 and the support leg 2 are welded together. The bottom of the support leg 2 is provided with a pad. Working principle: The measuring unit 11 feeds back data to the external central controller in real time to control the temperature control jacket 101 system to accurately adjust the flow rate of the heating / cooling medium and control the temperature. Locked at the optimal temperature for the enzyme, the composite stirring system 10 is activated to ensure full contact between the enzyme, raw materials, and water, and uniform dispersion of the materials, maximizing enzymatic hydrolysis efficiency. Once the reaction time is reached, the controller controls the jacket system to quickly switch to high-pressure steam mode. The detachable scraper 1003 removes the sticky layer on the tank wall, ensuring that heat is rapidly transferred to the materials inside the tank, raising the temperature to above a specific temperature in a very short time, achieving rapid and complete inactivation of the enzyme, and preventing further degradation of chlorophyll. After the enzyme is inactivated and cooled to a safe temperature, stirring is stopped, and the valve connecting to the bottom integrated solid-liquid separation module 7 is opened. The materials pass through the detachable porous filter plate 701 under their own gravity, and the filtrate is discharged and collected through the outlet on the side of the integrated solid-liquid separation module 7. The residue is trapped above the filter plate. This integrated operation eliminates the material transfer link, reducing oxidation risk and material loss. Specific Implementation Example 2: Reference Figure 1The device is equipped with a jacketed enzymatic hydrolysis storage tank for mixing and temperature control of initial materials and enzymes to ensure optimal hydrolysis conditions. A circulating pump continuously draws material from the storage tank and delivers it to the membrane separation unit, providing the driving force. A dynamic cross-flow membrane module employs a cross-flow mode, with an internal mechanical rotor or vibrator to create high-speed turbulent flow of the feed solution across the membrane surface. Piping and valves connect the storage tank, pump, and membrane module, and a return pipeline is provided. This device operates in a dynamic, circulating mode, reacting and separating simultaneously. Plant slurry and enzyme solution undergo enzymatic hydrolysis in the storage tank, while precise temperature control is achieved through the jacket. The circulating pump continuously extracts the liquid from the tank and sends it to the dynamic cross-flow membrane module. The high-speed rotating membrane module effectively prevents the formation of a filter cake layer on the membrane surface due to the high viscosity of the enzymatic hydrolysate, achieving efficient and stable solid-liquid separation. The filtrate produced by membrane separation is rich in chlorophyll and is extracted as a product. The concentrate that does not permeate the membrane is immediately sent back to the storage tank through the return pipeline for secondary or multiple enzymatic hydrolysis. This scheme integrates enzymatic hydrolysis and separation in the same circulation system, significantly shortening the overall process time. The dynamic membrane technology overcomes the problem of easy clogging in traditional static filtration, achieving continuous and efficient separation of chlorophyll solution with high yield.

[0021] In summary: 1. The use of a stirring system 10 achieves the use of a composite stirring system 10. The wall scraper can continuously remove sticky materials on the inner wall, eliminate heat transfer dead zones, and ensure efficient heat exchange between the material and the heating medium. This makes the temperature field in the reaction tank 1 highly uniform and stable within the optimal activity temperature range of the enzyme. This uniformity and stability, combined with the full macroscopic mixing effect of the blades, ensures comprehensive and continuous contact between the enzyme and the substrate, thereby shortening the enzymatic hydrolysis reaction time and significantly improving the cell wall rupture rate and chlorophyll extraction yield. 2. By adopting a temperature control jacket 101 and a stirring system 10, the double-layer high-efficiency temperature control jacket 101, combined with the real-time wall scraping action of the stirrer, can achieve rapid and precise heating of the reaction temperature. The temperature can be raised to the enzyme inactivation temperature in a very short time. This rapid thermal shock can immediately terminate enzyme activity, avoid degradation of chlorophyll caused by prolonged residence at high temperature, and ultimately ensure the high purity and high stability of chlorophyll in the extract, thereby enhancing the market value of the final product. 3. By adopting reaction vessel 1 and integrated solid-liquid separation module 7, the device directly sets up a detachable microporous filter plate assembly at the bottom of the vessel, so that the three key steps of enzymatic hydrolysis, inactivation and preliminary solid-liquid separation can be completed in one stop in the same equipment. This integrated operation significantly shortens the batch production time, reduces the pumping energy consumption required for material transfer, and fundamentally eliminates the risk of loss and contamination during the material transfer process.

[0022] In this invention, unless otherwise explicitly specified and limited, "above" or "below" the second feature can include direct contact between the first and second features, or contact between the first and second features through another feature between them. Furthermore, "above," "over," and "on top" of the second feature includes the first feature directly above or diagonally above the second feature, or simply indicates that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature includes the first feature directly below or diagonally below the second feature, or simply indicates that the first feature is at a lower horizontal level than the second feature.

[0023] The foregoing has shown and described the basic principles, main features, and advantages of this utility model. Those skilled in the art should understand that this utility model is not limited to the above embodiments. The embodiments and descriptions in the specification are merely preferred examples and are not intended to limit the utility model. Various changes and modifications can be made to this utility model without departing from its spirit and scope, and all such changes and modifications fall within the scope of the claimed utility model. The scope of protection of this utility model is defined by the appended claims and their equivalents.

Claims

1. A multifunctional enzymatic hydrolysis reaction device suitable for chlorophyll extraction, comprising a reaction vessel (1), characterized in that: The reaction tank (1) is fixedly provided with support legs (2) on both sides. The reaction tank (1) is fixedly provided with a motor (3) at the top center. The motor (3) is provided with a feed inlet (4) on one side. The reaction tank (1) is provided with a discharge port (5) at the bottom center. The discharge port (5) is provided with a discharge valve (6) at the bottom. The discharge valve (6) is detachably connected to an integrated solid-liquid separation module (7). The reaction tank (1) is provided with a stirring system (10) at the internal center. The stirring system (10) includes a vertically arranged rotating rod (1001). The rotating rod (1001) is horizontally fixed with a connecting rod (1002) on its side. The connecting rod (1002) is provided with a detachable scraper (1003) at its port.

2. The multifunctional enzymatic hydrolysis reaction device for chlorophyll extraction according to claim 1, characterized in that: The integrated solid-liquid separation module (7) has a residue discharge port (8) at the bottom and a filtrate outlet (9) on one side of the residue discharge port (8).

3. The multifunctional enzymatic hydrolysis reaction device for chlorophyll extraction according to claim 1, characterized in that: The reaction vessel (1) is equipped with a measuring unit (11) at its internal top.

4. The multifunctional enzymatic hydrolysis reaction device for chlorophyll extraction according to claim 1, characterized in that: The bottom end of the rotary rod (1001) is fixed with a paddle-type main stirrer (1004).

5. A multifunctional enzymatic hydrolysis reaction device suitable for chlorophyll extraction according to claim 4, characterized in that: The rotating rod (1001) passes through the top of the reaction vessel (1) and is connected to the motor (3) for transmission. The rotating rod (1001) is welded to the connecting rod (1002). The end face of the detachable scraper (1003) is chamfered.

6. A multifunctional enzymatic hydrolysis reaction device suitable for chlorophyll extraction according to claim 1, characterized in that: The inner wall of the reaction vessel (1) is provided with a temperature control jacket (101), and the interior of the integrated solid-liquid separation module (7) is provided with a detachable porous filter plate (701).

7. A multifunctional enzymatic hydrolysis reaction device suitable for chlorophyll extraction according to claim 1, characterized in that: The reaction vessel (1) and the support leg (2) are connected by welding, and the bottom of the support leg (2) is provided with a pad.

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