Floor cleaning agent bio-enzyme preparation device

Abstract

The utility model relates to the field of biological enzyme preparation, specifically disclose a floor cleaning agent biological enzyme preparation device, including fermentation tank, the upper baffle and lower baffle are equipped with respectively at fermentation tank top and bottom, be equipped with rotating shaft in the fermentation tank, the rotating shaft inner sleeve is equipped with with its rotation cooperation's adjusting screw rod, rotating shaft is equipped with with adjusting screw rod screw joint and can along the rotating shaft inner wall up and down reciprocating sliding's sliding block, sliding block is equipped with with its rotation cooperation's adjusting arm, adjusting arm bottom is equipped with with its rotation cooperation's push block, push block bottom is equipped with with its sliding cooperation's stirring oar, stirring oar one side movable riveting in rotating shaft inner wall, stirring oar inside still embeds and is equipped with with push block synchronous movement's extension paddle, through the accurate angle adjustment of stirring oar 15~70 and the length adjustment of extension paddle, realize the stirring on demand, adapt period and keep the body, logarithm period and dissolve oxygen, late stage clear precipitate, solve the poor adaptability problem when using the conventional fixed angle paddle.

Description

Technical Field

[0001] This utility model relates to the field of bio-enzyme preparation, and in particular to a bio-enzyme preparation device for floor cleaners. Background Technology

[0002] The bio-enzyme preparation device for floor cleaners is a complete set of equipment specifically designed for fermenting, extracting, and purifying bio-enzymes, such as proteases and lipases. The final bio-enzymes produced can serve as the core active ingredients of floor cleaners, achieving highly efficient stain removal and easy biodegradability. The core equipment is a fermentation tank. Culture medium and microbial strains are added to the fermentation tank, and by controlling the temperature, pH value, dissolved oxygen, and stirring speed, the microorganisms are allowed to multiply in large quantities and secrete the target bio-enzymes in a suitable environment.

[0003] As the source of bio-enzyme synthesis, the fermentation process directly determines the enzyme yield, activity, and subsequent application effects. Therefore, the performance of the fermenter is crucial to the entire preparation process. As the core component for material mixing, the stirring mechanism needs to achieve a uniform distribution of nutrients and oxygen in the fermentation broth through reasonable stirring intensity and range. This avoids imbalances in cell growth and hinders enzyme synthesis caused by local concentration gradients or insufficient dissolved oxygen. The stirring requirements of microorganisms vary significantly at different fermentation stages. Gentle stirring is required during the logarithmic phase to reduce cell shear damage, while increased stirring intensity is needed during the stationary phase to improve enzyme synthesis efficiency. In the later stages of enzyme production, the stirring range needs to be adjusted to adapt to changes in the viscosity of the fermentation broth.

[0004] The length and installation angle of the stirring blades in current fermentation tanks are fixed, making it impossible to flexibly adjust the stirring effect according to the dynamic changes in the fermentation stage. This can lead to discrepancies between the stirring requirements and the actual situation. For example, excessive stirring in the early stage of fermentation can damage the activity of the microorganisms, while insufficient stirring in the later stage can cause local nutrient depletion and low dissolved oxygen in the tank. This directly leads to an imbalance in the uniformity of the microbial growth environment, which not only reduces the synthesis efficiency and enzyme activity recovery rate of biological enzymes, but also affects the stability of the cleaning performance of subsequent floor cleaners.

[0005] The technical problem to be solved in this application is: how to adjust the radial coverage range and axial stirring height of the impeller according to the fermentation of microorganisms to achieve on-demand stirring. Utility Model Content

[0006] In order to overcome the shortcomings of the existing technology, the purpose of this utility model is to provide a floor cleaner bio-enzyme preparation device with adjustable stirring blade length and angle, which can adapt to the stirring needs of different fermentation stages of microorganisms to ensure the uniformity of the growth environment, thereby improving the efficiency and quality of bio-enzyme preparation.

[0007] The technical solution adopted by this utility model is as follows: a biological enzyme preparation device for floor cleaner, including a fermentation tank, with an upper cover plate and a lower cover plate respectively at the top and bottom of the fermentation tank. A rotating shaft is provided inside the fermentation tank, and an adjusting screw is fitted inside the rotating shaft to rotate with it. The rotating shaft is provided with a sliding block that is screwed to the adjusting screw and can slide up and down along the inner wall of the rotating shaft. The sliding block is provided with an adjusting arm that rotates with it, and a push block that rotates with it is provided at the bottom of the adjusting arm. A stirring paddle that slides with it is provided at the bottom of the push block. One side of the stirring paddle is movably riveted to the inner wall of the rotating shaft, and an extension paddle that moves synchronously with the push block is also embedded inside the stirring paddle.

[0008] When the adjusting screw rotates, it drives the screwed sliding block to slide up and down along the inner wall of the rotating shaft. The sliding block pushes the push block to move through the adjusting arm. The push block drives the stirring paddle to rotate around the riveting point on the inner wall of the rotating shaft to adjust the angle. At the same time, the push block drives the extension paddle to extend and retract within the stirring paddle to adjust the length. By simultaneously adjusting the angle of the stirring paddle and the length of the extension paddle, the stirring intensity and range requirements of different fermentation stages of microorganisms can be adapted to ensure uniform distribution of nutrients and dissolved oxygen in the fermenter and improve the yield and activity of biological enzymes.

[0009] In some embodiments, a groove is provided at the top of the stirring paddle, and the bottom of the pusher block passes through the groove and is fixedly connected to one end of the extension paddle that extends into the stirring paddle; when the pusher block slides in the groove, it not only provides guidance for the angle adjustment of the stirring paddle, but also drives the extension paddle to extend and retract synchronously through the fixed connection, ensuring the linkage between the angle adjustment of the stirring paddle and the length adjustment of the extension paddle.

[0010] In some embodiments, at least two adjusting arms are provided in the lateral direction. Each adjusting arm is connected to a set of sliding blocks, push blocks, stirring paddles and extension paddles. The adjusting screw, adjusting arms and stirring paddles are triangular in structure. The multiple adjusting arms and corresponding components distributed laterally, together with the stability of the triangular structure, make the driving force of the sliding blocks evenly transmitted to each stirring paddle, ensuring that multiple sets of stirring components are adjusted synchronously. This improves the force balance of the stirring system, avoids deformation of the rotating shaft or adjusting screw caused by one-sided weight imbalance, and expands the lateral stirring range, reducing the stirring dead zone in the fermenter.

[0011] In some embodiments, at least three pairs of stirring paddles are provided along the longitudinal direction, and each pair of stirring paddles is symmetrically distributed about the adjusting screw; when the multiple pairs of symmetrically distributed stirring paddles are rotated by the rotating shaft, they can cover different height areas in the fermenter, forming a three-dimensional stirring space in conjunction with the lateral distribution; eliminating the concentration and dissolved oxygen gradient between the upper and lower layers in the tank, ensuring that the microbial growth environment is consistent in each height layer, and promoting uniform cell reproduction and enzyme secretion.

[0012] In some embodiments, the angle of the stirring paddle can be adjusted to 15°–30°, 30°–40°, 40°–50°, and 60°–70°, corresponding to the enzyme adaptation phase, logarithmic growth phase, enzyme secretion phase, and late fermentation phase, respectively. By adjusting the stirring paddle to the corresponding angle to suit the metabolic characteristics of microorganisms at different fermentation stages, a smaller angle during the adaptation phase reduces cell shear damage; a medium angle during the logarithmic growth phase balances nutrient mixing and cell protection; a larger angle during the enzyme secretion phase improves material exchange efficiency; and a larger angle in the late fermentation phase adapts to the stirring requirements of high-viscosity fermentation broth. Precise angle adjustment optimizes the microbial growth environment at each stage, improving the efficiency and activity stability of biological enzymes.

[0013] In some embodiments, a stirring motor is mounted on the top of the rotating shaft, and an adjusting motor is mounted on the top of the adjusting screw. The stirring motor and the adjusting motor are arranged vertically and synchronously, and the rotating shaft and the adjusting screw rotate coaxially.

[0014] In some embodiments, an annular gas distribution pipe is fitted on the outer side of the fermenter, and the annular gas distribution pipe is provided with multiple gas nozzles that connect to the inside of the fermenter.

[0015] In some embodiments, multiple gas nozzles are provided with gas guide tubes connected in series, and the gas guide tubes are also provided with gas filling ports; sterile gas can enter the gas guide tubes along the gas filling ports and be sprayed out from the gas nozzles, agitating the sediment at the bottom of the fermentation broth to roll and prevent precipitation.

[0016] In some embodiments, the upper cover plate is provided with a microbial inlet and a culture medium inlet, and the lower cover plate is provided with a discharge outlet.

[0017] The beneficial effects of this utility model are as follows: by adjusting the precise angle of the stirring paddle from 15° to 70° and extending the length of the paddle, stirring can be achieved on demand, preserving bacteria during the adaptation period, increasing dissolved oxygen during the logarithmic period, and clearing sediment in the later stage, thus solving the problem of poor adaptability when using traditional fixed-angle paddles.

[0018] The combination of a horizontal triangular structure and a vertically symmetrically distributed stirring component, along with dual adjustment of angle and length, creates a three-dimensional stirring space. This eliminates the concentration and dissolved oxygen gradient between the upper and lower layers of the tank, improving stirring efficiency, bio-enzyme production, and total energy consumption, while ensuring enzyme activity retention. Attached Figure Description

[0019] Figure 1 This is a schematic diagram of the overall three-dimensional structure of the present invention;

[0020] Figure 2 This is a schematic cross-sectional view of the internal structure of the fermenter of this utility model;

[0021] Figure 3 For the present utility model Figure 2 A magnified view of the structure at point A in the middle;

[0022] Figure 4 This is a cross-sectional view of the annular gas distribution pipe of this utility model.

[0023] In the diagram: 1. Fermentation tank; 11. Top cover plate; 111. Inoculum inlet; 112. Culture medium inlet; 12. Bottom cover plate; 121. Outlet; 2. Annular gas distribution pipe; 21. Gas supply pipe; 22. Gas guide pipe; 23. Gas nozzle; 3. Rotating shaft; 30. Stirring motor; 31. Sliding block; 32. Adjusting arm; 321. Push block; 33. Stirring paddle; 331. Slide groove; 34. Extension paddle; 4. Adjusting screw; 40. Adjusting motor. Detailed Implementation

[0024] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0025] Please see Figure 1 This utility model provides a technical solution: a biological enzyme preparation device for floor cleaner, including a fermentation tank 1. The fermentation tank 1 is a vertical cylindrical structure with an upper cover plate 11 sealed at the top and a lower cover plate 12 sealed at the bottom. The upper cover plate 11 is provided with a microbial inlet 111 and a culture medium inlet 112 for independently adding microbial inlets and culture medium. The lower cover plate 12 is provided with an outlet 121 in the center for discharging the enzyme solution after fermentation.

[0026] Please see Figure 2 and 3The stirring and regulating system is the core functional component, located inside the fermentation tank 1. It includes a hollow rotating shaft 3, an adjusting screw 4 coaxially sleeved inside the rotating shaft 3, a sliding block 31 inside the rotating shaft 3, at least two adjusting arms 32 arranged laterally along the rotating shaft 3, a long, strip-shaped stirring paddle 33, a pusher block 321, and an extension paddle 34 embedded inside the stirring paddle 33. The rotating shaft 3 is vertically arranged along the axis of the fermentation tank 1, with an upper cover plate 11 extending from its top and connected to a stirring motor 30. The stirring motor 30 drives the shaft to rotate around its axis. The adjusting screw 4 rotates in conjunction with the rotating shaft 3. The upper cover plate 11 extends from its top and connects to a regulating motor 40. The stirring motor 30 and the regulating motor 40 are vertically and synchronously arranged, enabling independent or coaxial rotation of the rotating shaft 3 and the adjusting screw 4. The sliding block... The inner wall of the adjustment arm 31 is provided with an internal thread that is compatible with the adjusting screw 4. The screw connection enables the arm to slide back and forth along the inner wall of the rotating shaft 3. The two ends of the adjusting arm 32 are respectively connected to the sliding block 31 and the push block 321 through pins. The adjusting screw 4, the adjusting arm 32 and the stirring paddle 33 form a triangular structure to improve the stability of the driving force transmission. One side of the stirring paddle 33 is movably riveted to the inner wall of the rotating shaft 3 through a pin. The top is provided with a sliding groove 331 extending along the length direction. At least three pairs of grooves are provided along the longitudinal direction of the rotating shaft 3. Each pair is symmetrically distributed about the adjusting screw 4 to form a three-dimensional stirring space. The bottom of the push block 321 passes through the sliding groove 331 and slides in cooperation with the sliding groove 331. The end of the extension paddle 34 that extends into the stirring paddle 33 is fixedly connected to the bottom of the push block 321 to achieve synchronous adjustment of length and angle.

[0027] The stirring paddle 33 can rotate precisely within a range of 15° to 70° around the riveting point on the inner wall of the rotating shaft 3. When the angle is between 15° and 30°, the stirring paddle 33 is close to the rotating shaft 3 and is in a contracted state, rotating only in the central area near the rotating shaft 3. The fermentation broth mainly forms a small axial circulation, with the vertical flow limited to the middle of the tank. The stirring in the tank wall and bottom areas is weaker. This state is suitable for the early stage of fermentation, i.e., the adaptation period, with a fermentation time of about 0 to 12 hours. It can avoid damage to the fragile cells that have just been inoculated with low shear force, helping the cells to initially adapt to the environment. Moreover, because the stirring paddle 33 has a small force-bearing area and low rotational resistance, it has the lowest energy consumption and is suitable for viscosities ≤100 cP and cell densities ≤10. 6 The fermentation broth has a concentration of CFU / mL. When the angle is adjusted to 30°–50°, the agitator 33 is partially extended, radially covering two-thirds of the tank and axially covering the lower and middle parts. The fermentation broth forms a combined axial and radial circulation, flowing up and down along the agitator 33 and diffusing towards the tank wall. It also tumbles the fermentation broth at the bottom of the tank. Combined with multiple pairs of longitudinally symmetrically distributed agitators 33, dead zones between the upper and lower layers can be eliminated. This state corresponds to the logarithmic growth phase (12–48 h) and the enzyme secretion phase (48–72 h), ensuring a cell density ≥10⁻⁶ cells / mL during these stages. 8 ~10 9The high oxygen demand (CFU / mL) and uniform distribution of oxygen and nutrients prevent localized hypoxia or nutrient overload. The medium shear force does not damage the enzyme protein structure. Dissolved oxygen rate is increased by 20%–30% compared to smaller angles. Temperature and pH uniformity within the tank are optimal, with deviations ≤0.2℃ / 0.1pH. Sufficient contact between substrate and cells ensures a 15%–25% increase in enzyme yield. When the angle is increased to 60°–70°, the agitator 33 extends nearly horizontally, radially covering the entire tank, including the tank wall edges, and axially covering the lower part and bottom. This creates a strong radial diffusion of the fermentation broth combined with bottom scouring flow. The agitator 33 directly scours the tank wall and carries the sediment from the bottom upwards. With the extension paddle 34, the stirring range can be further expanded, making it suitable for the later stage of fermentation, i.e., after 72 hours, during the enzyme concentration stage. It can address the issues of cell flocculation or substrate precipitation caused by the increased viscosity of the fermentation broth (300-500 cP) and increased enzyme concentration. High shear force breaks down flocculation, and bottom rinsing prevents sediment agglomeration, ensuring the uniformity of the enzyme solution after subsequent separation and purification. Complete elimination of stagnant areas on the tank walls prevents scaling of the culture medium, achieving a bottom sediment removal rate of over 90%. The uniformity of the fermentation broth after stirring reaches over 95%. Only the usage time needs to be controlled, at 1-2 hours per batch, to avoid damage to sensitive cells due to high shear force during prolonged use. This stage also has the highest energy consumption. It is 20%-25% higher than the mid-angle.

[0028] When the pusher block 321 slides, it will drive the extension paddle 34 to extend and retract within the stirring paddle 33 through the fixed connection. When the small angle is adjusted, the extension paddle 34 retracts, which can reduce the stirring resistance in the central area. When the medium and large angles are adjusted, the extension paddle 34 extends, which can further expand the radial coverage range, enhance the stirring effect of the whole tank, and achieve synchronous adaptation of angle and length. At the same time, the stirring motor 30 drives the rotating shaft 3 to rotate, which drives the adjusted stirring paddle 33 and the extension paddle 34 to rotate synchronously.

[0029] Please see Figure 1 and 4 An annular gas distribution pipe 2 is fitted on the outer side of the fermenter 1. The annular gas distribution pipe 2 connects multiple gas nozzles 23 in series through a gas guide pipe 22. One end of the gas guide pipe 22 extending out of the fermenter 1 is provided with a gas inlet 21. The gas nozzles 23 penetrate the side wall of the fermenter 1 and extend into the interior. After sterile gas is introduced through the gas inlet 21, it is distributed to each gas nozzle 23 through the gas guide pipe 22 and sprayed out. Together with the stirring action, it increases the dissolved oxygen content and further prevents sedimentation at the bottom of the tank.

[0030] Finally, it should be noted that the above description is merely a preferred embodiment of the present utility model and is not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A bio-enzyme preparation device for floor cleaner, comprising a fermentation tank (1), wherein the fermentation tank (1) is provided with an upper cover plate (11) at the top and a lower cover plate (12) at the bottom, characterized in that, The fermenter (1) is provided with a rotating shaft (3), and an adjusting screw (4) is fitted inside the rotating shaft (3) to rotate with it. The rotating shaft (3) is provided with a sliding block (31) that is screwed to the adjusting screw (4) and can slide up and down along the inner wall of the rotating shaft (3). The sliding block (31) is provided with an adjusting arm (32) to rotate with it. The bottom of the adjusting arm (32) is provided with a push block (321) to rotate with it. The bottom of the push block (321) is provided with a stirring paddle (33) to slide with it. One side of the stirring paddle (33) is movably riveted to the inner wall of the rotating shaft (3). An extension paddle (34) that moves synchronously with the push block (321) is also embedded inside the stirring paddle (33).

2. The bio-enzyme preparation device for floor cleaner according to claim 1, characterized in that, The top of the stirring paddle (33) is provided with a groove (331), and the bottom of the pusher block (321) passes through the groove (331) and is fixedly connected to one end of the extension paddle (34) that extends into the stirring paddle (33).

3. The bio-enzyme preparation device for floor cleaner according to claim 1, characterized in that, At least two adjustment arms (32) are provided in the transverse direction. Each adjustment arm (32) is connected to a set of sliding blocks (31), push blocks (321), stirring paddles (33) and extension paddles (34). The adjustment screw (4), adjustment arms (32) and stirring paddles (33) are triangular in shape.

4. The bio-enzyme preparation device for floor cleaner according to claim 1, characterized in that, The stirring paddle (33) is provided in at least three pairs along the longitudinal direction, and each pair of stirring paddles (33) is symmetrically distributed about the adjusting screw (4).

5. The bio-enzyme preparation device for floor cleaner according to claim 1, characterized in that, The angle of the stirring paddle (33) can be adjusted to 15°~30°, 30°~40°, 40°~50° and 60°~70°, respectively, which correspond to the enzyme adaptation period, logarithmic growth period, enzyme secretion period and late fermentation period in the fermentation process.

6. The bio-enzyme preparation device for floor cleaner according to claim 1, characterized in that, A stirring motor (30) is installed on the top of the rotating shaft (3), and an adjusting motor (40) is installed on the top of the adjusting screw (4). The stirring motor (30) and the adjusting motor (40) are arranged vertically and synchronously, and the rotating shaft (3) and the adjusting screw (4) rotate coaxially.

7. The bio-enzyme preparation device for floor cleaner according to claim 1, characterized in that, The fermenter (1) is fitted with an annular gas distribution pipe (2) on its outer side, and the annular gas distribution pipe (2) is provided with a plurality of gas nozzles (23) that connect to the inside of the fermenter (1).

8. The bio-enzyme preparation device for floor cleaner according to claim 7, characterized in that, The plurality of gas nozzles (23) are provided with gas guide pipes (22) connected in series, and the gas guide pipes (22) are also provided with gas filling ports (21).

9. The bio-enzyme preparation device for floor cleaner according to claim 1, characterized in that, The upper cover plate (11) is provided with a microbial inlet (111) and a culture medium inlet (112), and the lower cover plate (12) is provided with an outlet (121).

Images