A device for collecting, separating and culturing pathogenic microorganisms

Abstract

The utility model relates to microorganism detection technical field especially relates to a pathogenic microorganism collection separation culture device, including bottom plate, the top fixedly connected with culture cylinder of bottom plate, the culture tray for cultivating microorganism is fixedly installed in culture cylinder, the top joint of culture cylinder is separated cylinder, the upper and lower ends of separated cylinder are equipped with silica gel pad for reducing mobile pollution, the top joint of separated cylinder is collected cylinder, the upper and lower ends of separated cylinder circumferential surface are equipped with a plurality of joint assembly for joint culture cylinder and collected cylinder, the inner wall fixedly connected with of separated cylinder is used for the preliminary filtration microorganism and is slanted filter screen, through the joint combination of culture cylinder, separated cylinder and collected cylinder on bottom plate, cooperate joint silica gel pad seal, form integrated closed channel, effectively reduce low concentration pathogenic microorganism leak detection risk, and the overall structure realizes quick assembly and disassembly through joint assembly, is convenient for cleaning and disinfection, improves the reliability and operating efficiency of pathogenic microorganism detection.

Description

Technical Field

[0001] This utility model relates to the field of microbial detection technology, and in particular to a device for collecting, separating and culturing pathogenic microorganisms. Background Technology

[0002] Microorganisms are a large group of organisms including bacteria, viruses, fungi, and some small protozoa, and are widely involved in many fields such as health, medicine, industry, agriculture, and environmental protection.

[0003] Existing technical solutions have obvious limitations. The operation process is fragmented, and the collection of samples requires the use of separate tools such as sampling tubes and inoculation loops. The samples are easily contaminated by the outside world during the transfer process, and the sample loss rate is high. In particular, it is easy to miss the detection when targeting low concentrations of pathogenic microorganisms. Furthermore, the separation accuracy is insufficient. Traditional streak separation requires manual control of the inoculation force and trajectory. The technical differences among different operators lead to inconsistent separation results and low single colony formation rate. Utility Model Content

[0004] In view of the problems of fragmented operation and easy contamination mentioned above, this utility model is proposed.

[0005] To solve the above-mentioned technical problems, this utility model provides the following technical solution: a pathogenic microorganism collection, separation and culture device, including a base plate, a culture tube fixedly connected to the top of the base plate, a culture tray for culturing microorganisms fixedly installed inside the culture tube, a separation tube snapped onto the top of the culture tube, silicone pads for reducing movement and contamination provided at both the upper and lower ends of the separation tube, a collection tube snapped onto the top of the separation tube, multiple snapping components for snapping the culture tube and the collection tube provided at both the upper and lower ends of the circumference of the separation tube, an inclined filter screen for preliminary filtration of microorganisms fixedly connected to the inner wall of the separation tube, a guide plate for guiding the movement of microorganisms after preliminary filtration provided below the inclined filter screen, a fine filter membrane for secondary filtration attached to the bottom end of the guide plate, and a negative pressure plate for generating negative pressure and discharging wastewater attached to the bottom end of the fine filter membrane.

[0006] As a preferred embodiment of the pathogenic microorganism collection, isolation and culture device of this utility model, the snap-fit ​​assembly includes an insert block, a semi-circular disk, a push rod, a fixing block, a tension spring and a mounting block. The insert block is fixedly connected to the inner wall of the mounting block, the front end of the insert block is rotatably connected to the circumferential surface of the semi-circular disk, the circumferential surface of the semi-circular disk is fixedly connected to the push rod, and the push rod penetrates the outer wall of the mounting block.

[0007] As a preferred embodiment of the pathogenic microorganism collection, isolation and culture device of this utility model, the inner wall of the fixing block and the mounting block are fixedly connected, the end face of the fixing block is perpendicular to the surface of the push rod, one end of the tension spring is welded to the end face of the fixing block, and the other end of the tension spring is welded to the surface of the push rod.

[0008] As a preferred embodiment of the pathogenic microorganism collection, isolation and culture device of this utility model, the bottom plate is provided with an installation groove, a heating plate is fixedly connected in the installation groove, a vent is installed above the heating plate, and the circumferential surface of the vent is fixedly connected to the inner annular surface of the installation groove.

[0009] As a preferred embodiment of the pathogenic microorganism collection, isolation and culture device of this utility model, the bottom end of the culture tray is attached to the top end of the vent hole, and the interior of the culture tray is provided with solid agar culture medium for culturing microorganisms.

[0010] As a preferred embodiment of the pathogenic microorganism collection, separation and culture device of this utility model, wherein: a drain pipe for drainage is fixedly connected to the bottom end of the negative pressure plate, and the drain pipe penetrates the outer wall of the separation cylinder.

[0011] The beneficial effects of this utility model are:

[0012] The integrated, sealed channel is formed by the snap-fit ​​combination of the culture tube, separation tube, and collection tube on the base plate, with silicone gaskets sealing the seams. Samples can be transferred from collection to separation without the need for separate tools, completely reducing external contamination and sample loss, and effectively reducing the risk of missed detection of low-concentration pathogens. The combination of the inclined filter screen, guide plate, and fine filter membrane inside the separation tube achieves orderly filtration of samples and enrichment of pathogens, eliminating the need for manual streaking and eliminating the influence of operator skill differences, ensuring stable and consistent separation results, and improving the single colony formation rate. The overall structure allows for quick assembly and disassembly through snap-fit ​​components, facilitating cleaning and disinfection, and improving the reliability and operational efficiency of pathogen detection. Attached Figure Description

[0013] To more clearly illustrate the technical solutions of the embodiments of this utility model, the drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort. Among them:

[0014] Figure 1 This is a schematic diagram of the overall structure of this utility model.

[0015] Figure 2 This is a schematic cross-sectional view of the overall structure of this utility model.

[0016] Figure 3 This is a schematic diagram of the internal structure of the snap-fit ​​assembly of this utility model.

[0017] Figure 4 This is a schematic diagram of the installation structure of the culture tray of this utility model.

[0018] Explanation of reference numerals in the attached drawings: 1. Base plate; 2. Culture cylinder; 3. Separation cylinder; 4. Collection cylinder; 5. Snap-fit ​​assembly; 6. Silicone pad; 7. Inclined filter screen; 8. Guide plate; 9. Fine filter membrane; 10. Negative pressure plate; 11. Drain pipe; 12. Culture tray; 13. Heating element; 14. Vent hole; 15. Insert block; 16. Semicircular disc; 17. Push rod; 18. Fixing block; 19. Tension spring; 20. Mounting block. Detailed Implementation

[0019] To make the above-mentioned objectives, features and advantages of this utility model more apparent and understandable, the specific embodiments of this utility model will be described in detail below with reference to the accompanying drawings.

[0020] Example 1

[0021] Reference Figures 1-3 This is the first embodiment of the present invention, which provides a pathogenic microorganism collection, separation and culture device, including a base plate 1, a culture cylinder 2 fixedly connected to the top of the base plate 1, a culture tray 12 for culturing microorganisms fixedly installed inside the culture cylinder 2, a separation cylinder 3 snapped onto the top of the culture cylinder 2, silicone pads 6 for reducing moving contamination provided at both the upper and lower ends of the separation cylinder 3, a collection cylinder 4 snapped onto the top of the separation cylinder 3, and multiple snapping components 5 for snapping the culture cylinder 2 and the collection cylinder 4 provided at both the upper and lower ends of the circumference of the separation cylinder 3, an inclined filter screen 7 for preliminary filtration of microorganisms fixedly connected to the inner wall of the separation cylinder 3, a guide plate 8 for guiding the movement of microorganisms after preliminary filtration provided below the inclined filter screen 7, a fine filter membrane 9 for secondary filtration attached to the bottom end of the guide plate 8, and a negative pressure plate 10 for generating negative pressure and discharging wastewater attached to the bottom end of the fine filter membrane 9.

[0022] The snap-fit ​​assembly 5 includes a plug 15, a semi-circular disk 16, a push rod 17, a fixing block 18, a tension spring 19, and a mounting block 20. The plug 15 is fixedly connected to the inner wall of the mounting block 20, the front end of the plug 15 is rotatably connected to the circumferential surface of the semi-circular disk 16, the circumferential surface of the semi-circular disk 16 is fixedly connected to the push rod 17, and the push rod 17 passes through the outer wall of the mounting block 20.

[0023] The fixing block 18 is fixedly connected to the inner wall of the mounting block 20. The end face of the fixing block 18 is perpendicular to the surface of the push rod 17. One end of the tension spring 19 is welded to the end face of the fixing block 18, and the other end of the tension spring 19 is welded to the surface of the push rod 17.

[0024] During use, align the mounting block 20 at the bottom of the separation cylinder 3 with the corresponding mounting block 20 at the top of the culture cylinder 2, and slowly insert it downwards. During insertion, the insert block 15 inside the culture cylinder 2 mounting block 20 presses the circumferential surface of the semi-circular disk 16 inside the separation cylinder 3 mounting block 20, simultaneously driving the fixedly connected push rod 17 to slide along the inner wall of the mounting block 20. The push rod 17 pulls the tension spring 19 welded to it, and the fixing block 18 at the fixed end of the tension spring 19 remains stationary. When the two mounting blocks 20 are completely in contact, and the silicone pad 6 at the bottom of the separation cylinder 3 is in close contact with the top of the culture cylinder 2, the semi-circular disk 16 of the separation cylinder 3 mounting block 20 and the semi-circular disk 16 of the culture cylinder 2 mounting block 20 are in contact and aligned. The tension spring 19 releases its elastic potential energy, pulls the push rod 17 to reset, and the push rod 17 drives the semi-circular disk 16 to rotate back to the initial angle. The two semi-circular disks 16 engage with each other, achieving a stable and fixed connection between the separation cylinder 3 and the culture cylinder 2.

[0025] Using the same method as above, align the bottom of the collection cylinder 4 with the top of the separation cylinder 3, and insert the mounting block 20 of the collection cylinder 4 and the mounting block 20 of the top of the separation cylinder 3. The entire device is then assembled, forming a closed collection-separation-cultivation channel.

[0026] When disassembly and cleaning are required, simultaneously push the push rods 17 on the two sets of mounting blocks 20 with both hands. The push rods 17 slide against the elastic force of the tension spring 19, causing the semi-circular disk 16 to rotate until the contact surfaces of the two semi-circular disks 16 are perpendicular, thus releasing the locking relationship. At this time, pull the collection tube 4 upward to separate it from the separation tube 3. Then pull the separation tube 3 upward to complete the disassembly from the culture tube 2. After disassembly, each component can be autoclaved or wiped for disinfection. Special attention should be paid to cleaning the gaps of the locking components 5 to avoid contamination by residual samples.

[0027] Example 2

[0028] Reference Figure 1 , Figure 2 and Figure 4 This is the second embodiment of the present invention. The difference between this embodiment and the first embodiment is that: a mounting groove is provided in the base plate 1, a heating element 13 is fixedly connected in the mounting groove, a vent hole 14 is installed above the heating element 13, and the circumferential surface of the vent hole 14 is fixedly connected to the inner annular surface of the mounting groove.

[0029] The bottom of the culture tray 12 is attached to the top of the vent 14, and the interior of the culture tray 12 is provided with solid agar culture medium for culturing microorganisms.

[0030] The bottom end of the negative pressure plate 10 is fixedly connected to a drain pipe 11 for drainage, which penetrates the outer wall of the separation cylinder 3.

[0031] During use, after the device is assembled, the power supply of the heating element 13 in the base plate 1 is turned on. The heat generated by the heating element 13 is evenly transferred to the culture tray 12 in the culture tube 2 through the vent 14, so that the solid agar culture medium maintains a suitable temperature for microbial growth. At the same time, the connection and sealing between the negative pressure plate 10 and the drain pipe 11 are checked, and the end of the drain pipe 11 is connected to the wastewater collection container.

[0032] The liquid sample to be tested is slowly poured into the collection cylinder 4. Under the action of gravity, the sample flows into the separation cylinder 3 below and first comes into contact with the inclined filter screen 7 fixed on the inner wall of the separation cylinder 3. The inclined filter screen 7 performs preliminary filtration on the sample, trapping larger impurities in the sample. The liquid containing pathogenic microorganisms flows down the inclined surface of the filter screen and enters the guide plate 8 below.

[0033] The guide plate 8 precisely directs the pre-filtered liquid to the fine filter membrane 9 attached to its bottom. The fine filter membrane 9 can trap pathogenic microbial cells in the sample, while allowing water and small molecule impurities to pass through. The vacuum pump built into the negative pressure plate 10 is activated, and the negative pressure plate 10 generates negative pressure suction below the fine filter membrane 9, accelerating liquid permeation. Pathogenic microbial cells are firmly attached to the fine filter membrane 9, and excess wastewater is discharged to the collection container through the drain pipe 11 at the bottom of the negative pressure plate 10, completing the separation and enrichment of microorganisms.

[0034] After separation, turn off the vacuum pump, remove the separation cylinder 3 according to the disassembly method described above, carefully remove the negative pressure plate 10, remove the fine filter membrane 9 with pathogenic microorganism cells attached from the bottom of the guide plate 8, open the sealing structure at the top of the culture cylinder 2, spread the fine filter membrane 9 flat on the surface of the solid agar medium in the culture tray 12, ensure that the fine filter membrane 9 is in close contact with the medium, re-cover the separation cylinder 3 and snap it in place, keep the heating plate 13 working continuously, maintain the constant temperature of the culture environment through the vent 14, after a certain number of hours of culture, clear single colonies of pathogenic microorganisms can be observed on the surface of the agar medium, which can be used for subsequent identification and detection.

[0035] After the culture is completed, turn off the power to the heating element 13, disassemble the device and thoroughly disinfect all components, replace the fine filter membrane 9 and the silicone pad 6, and prepare for the next test.

[0036] The remaining structure is the same as that in Example 1.

[0037] It should be noted that the above embodiments are only used to illustrate the technical solution of this utility model and are not intended to limit it. Although this utility model has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solution of this utility model without departing from the spirit and scope of the technical solution of this utility model, and all such modifications or substitutions should be covered within the scope of the claims of this utility model.

Claims

1. A pathogenic microorganism collection, isolation, and culture device, comprising a base plate (1), characterized in that: A culture tube (2) is fixedly connected to the top of the base plate (1). A culture tray (12) for culturing microorganisms is fixedly installed inside the culture tube (2). A separation tube (3) is snapped onto the top of the culture tube (2). Both the upper and lower ends of the separation tube (3) are provided with silicone pads (6) for reducing mobile contamination. A collection tube (4) is snapped onto the top of the separation tube (3). Both the upper and lower ends of the circumferential surface of the separation tube (3) are provided with multiple snapping components (5) for snapping onto the culture tube (2) and the collection tube (4). An inclined filter screen (7) for preliminary filtration of microorganisms is fixedly connected to the inner wall of the separation tube (3). A guide plate (8) for guiding the movement of microorganisms after preliminary filtration is provided below the inclined filter screen (7). A fine filter membrane (9) for secondary filtration is attached to the bottom end of the guide plate (8). A negative pressure plate (10) for generating negative pressure and discharging wastewater is attached to the bottom end of the fine filter membrane (9).

2. The pathogenic microorganism collection, isolation, and culture device according to claim 1, characterized in that: The snap-fit ​​assembly (5) includes a plug (15), a semi-circular disk (16), a push rod (17), a fixing block (18), a tension spring (19), and a mounting block (20). The plug (15) is fixedly connected to the inner wall of the mounting block (20). The front end of the plug (15) is rotatably connected to the circumferential surface of the semi-circular disk (16). The circumferential surface of the semi-circular disk (16) is fixedly connected to the push rod (17). The push rod (17) penetrates the outer wall of the mounting block (20).

3. The pathogenic microorganism collection, isolation, and culture device according to claim 2, characterized in that: The fixing block (18) is fixedly connected to the inner wall of the mounting block (20). The end face of the fixing block (18) is perpendicular to the surface of the push rod (17). One end of the tension spring (19) is welded to the end face of the fixing block (18), and the other end of the tension spring (19) is welded to the surface of the push rod (17).

4. The pathogenic microorganism collection, isolation, and culture device according to claim 1, characterized in that: The base plate (1) has an installation groove, and a heating element (13) is fixedly connected in the installation groove. A vent hole (14) is installed above the heating element (13), and the circumferential surface of the vent hole (14) is fixedly connected to the inner annular surface of the installation groove.

5. The pathogenic microorganism collection, isolation, and culture device according to claim 4, characterized in that: The bottom end of the culture tray (12) is attached to the top end of the vent (14), and the interior of the culture tray (12) is provided with a solid agar culture medium for culturing microorganisms.

6. The pathogenic microorganism collection, isolation, and culture device according to claim 1, characterized in that: The bottom end of the negative pressure plate (10) is fixedly connected to a drain pipe (11) for draining water, and the drain pipe (11) penetrates the outer wall of the separation cylinder (3).

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