A device for gradient stress domestication of salt-tolerant microorganisms

Abstract

The utility model belongs to the field of microorganism culture technology, especially relate to a salt-tolerant microorganism gradient stress domestication device, including nutrient tank, box and gas mixing box, the inside fixed coupling of nutrient tank has water pump, the water suction end fixed communication of water pump has water suction pipe, the water outlet fixed communication of water pump has water outlet pipe. Through accurate control nutrition solution, salinity and gas and so on condition, can realize the step by step gradient stress domestication, designed the closed gas circulation passage organic volatile gas through the first gas pump import gas mixing box, and with the dry air of second gas pump, after mixing, controllably back to the filler box, this greatly reduces the problem that irritant or harmful volatile organic compounds diffuse to the laboratory air, guarantees the health of experimental personnel, and can improve the bioavailability of hydrophobic organic matter, and the filler box provides huge biofilm for microorganism, and this culture mode is not easy to lose microorganism.

Description

Technical Field

[0001] This invention belongs to the field of microbial culture technology, and in particular relates to a salt-tolerant microbial gradient stress acclimatization device. Background Technology

[0002] Microorganisms refer to tiny organisms that are widely present in nature, are small in size, have simple structures, cannot be observed directly with the naked eye, and can only be observed by magnification of hundreds, thousands, or even tens of thousands of times using an optical or electron microscope. According to their size, presence or absence of basic cellular structure, degree of differentiation of cell nucleus, and nucleic acid composition, they can be divided into three categories: eukaryotic microorganisms, prokaryotic microorganisms, and acellular microorganisms.

[0003] Traditional acclimatization methods include direct addition and shake-flask acclimatization. Direct addition involves adding pollutants directly to activated sludge. However, since many volatile organic compounds are hydrophobic and highly volatile, direct addition cannot enable microorganisms to utilize organic matter effectively. Furthermore, the pungent odor emitted can affect the normal operation of the laboratory. In addition, shake-flask acclimatization produces a small number of microbial communities each time, requiring enrichment before inoculation, which is complex and has a long cultivation and acclimatization cycle. Therefore, we propose a salt-tolerant microbial gradient stress acclimatization device. Utility Model Content

[0004] The purpose of this invention is to provide a salt-tolerant microbial gradient stress acclimatization device to solve the problems mentioned in the background art.

[0005] In view of this, the present invention provides a salt-tolerant microbial gradient stress acclimatization device, including a nutrient tank, a box body, and a gas mixing box. A water pump is fixedly connected inside the nutrient tank. The water pump's suction end is fixedly connected to a suction pipe, and the water pump's outlet end is fixedly connected to an outlet pipe. A packing box is fixedly connected inside the box body. The outlet end of the outlet pipe passes through the nutrient tank, the box body, and the packing box in sequence and extends into the interior of the packing box. Four support legs are fixedly connected to the bottom end of the box body.

[0006] In the above technical solution, the gas mixing box is further provided with a first gas pipe, and one end of the first gas pipe is provided with an organic gas generator.

[0007] In the above technical solution, the first gas tube output end passes through the organic gas generator and extends to the outside, and one end of the first gas tube is fixedly connected to a first gas pump.

[0008] In the above technical solution, the output end of the first air pump is connected to the inside of the first air pipe, and the outer surface of the gas mixing box is fixedly connected to the second air pump.

[0009] In the above technical solution, a gas dryer is fixedly connected to the outer surface of the second air pump, and a second air pipe is fixedly connected to the output end of the second air pump.

[0010] In the above technical solution, one end of the second gas pipe passes through the gas dryer and extends into the interior of the packing box, and an injection pipe is fixedly connected to the outer surface of the gas mixing box.

[0011] In the above technical solution, a valve is fixedly installed on the outer surface of the injection pipe, and a rotor flow meter is fixedly connected to the outer surface of the first gas pipe.

[0012] In this technical solution,

[0013] The beneficial effects of this utility model are:

[0014] 1. This salt-tolerant microbial gradient stress acclimatization device can achieve gradual gradient stress acclimatization by precisely controlling conditions such as nutrient solution, salinity, and gas. It is designed with a closed gas circulation path. Volatile organic gases are introduced into the gas mixing box through the first gas pump and mixed with dry air delivered by the second gas pump. After mixing, the mixture is controllably returned to the packing box. This greatly reduces the problem of irritating or harmful volatile organic compounds escaping into the laboratory air, protects the health of laboratory personnel, and may improve the bioavailability of hydrophobic organic matter.

[0015] 2. This salt-tolerant microbial gradient stress acclimatization device provides a large attachment surface for microorganisms in the packing box, which can form a high-biomass biofilm. This immobilized culture method is superior to the suspension culture of the shake flask method. Microorganisms are not easily lost, the cell density is high, and the acclimatized microbial community is more diverse and stable. This eliminates the tedious enrichment steps, which is expected to shorten the acclimatization cycle and directly provide sufficient strains for subsequent scale-up experiments. Attached Figure Description

[0016] Figure 1 This is a three-dimensional structural diagram of the present invention;

[0017] Figure 2 This is a schematic diagram of the rear view structure of this utility model;

[0018] Figure 3 This is a top view of the structure of this utility model;

[0019] Figure 4 This is a side view of the structure of this utility model.

[0020] The markings in the diagram are as follows:

[0021] 1. Nutrient tank; 2. Box body; 3. Gas mixing box; 4. Water outlet pipe; 5. Packing box; 6. First air pump; 7. Organic gas generator; 8. First air pipe; 9. Second air pump; 10. Gas dryer; 11. Injection pipe; 12. Valve; 13. Second air pipe; 14. Support leg; 15. Rotor flow meter; 16. Water pump; 17. Suction pipe. Detailed Implementation

[0022] The following is in conjunction with the appendix Figures 1-4 This application will be described in further detail.

[0023] In this application, the terms "upper," "lower," "left," "right," "front," "rear," "top," "bottom," "inner," "outer," "middle," "vertical," and "horizontal," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. These terms are primarily for the purpose of better describing this application and its embodiments, and are not intended to limit the indicated device, element, or component to having a specific orientation, or to be constructed and operated in a specific orientation.

[0024] Example 1: This example provides a salt-tolerant microbial gradient stress acclimatization device, including a nutrient tank 1, a box 2 and a gas mixing box 3. A water pump 16 is fixedly connected inside the nutrient tank 1. A water suction pipe 17 is fixedly connected to the water suction end of the water pump 16. A water outlet pipe 4 is fixedly connected to the water outlet end of the water pump 16. A packing box 5 is fixedly connected inside the box 2. The water outlet end of the water outlet pipe 4 passes through the nutrient tank 1, the box 2 and the packing box 5 in sequence and extends into the interior of the packing box 5. Four support legs 14 are fixedly connected to the bottom of the box 2.

[0025] The nutrient tank 1 is the core reaction zone, used to store nutrient solution and microorganisms. It is the starting point and circulation center of the domestication process. The water pump 16 provides liquid circulation power for the system, ensuring uniform mixing and delivery of nutrients and microorganisms. The suction pipe 17 is the channel through which the water pump 16 draws liquid from the nutrient tank 1. The outlet pipe 4 is the channel through which the nutrient solution is delivered from the water pump 16 to the packing box 5. The box body 2 is the main support structure and outer protective shell of the device. The packing box 5 is the core place for microorganisms to attach and grow. Its internal packing provides a carrier for microorganisms to form a high-concentration biofilm.

[0026] Example 2: This example provides a salt-tolerant microbial gradient stress acclimatization device. In addition to the technical solutions of the above examples, it also has the following technical features: a first gas pipe 8 is fixedly connected inside the gas mixing box 3, and an organic gas generator 7 is fixedly connected to one end of the first gas pipe 8.

[0027] Among them, the gas mixing box 3 uniformly mixes different gases to provide a specific and stable gaseous environment for microorganisms, and the first gas pipe 8 is a pipe that delivers volatile organic gases or draws back gases from the packing box 5.

[0028] Example 3: This example provides a salt-tolerant microbial gradient stress acclimatization device. In addition to the technical solutions of the above examples, it also has the following technical features: the output end of the first air pipe 8 passes through the organic gas generator 7 and extends to the outside, and one end of the first air pipe 8 is fixedly connected to the first air pump 6.

[0029] The first air pump 6 provides power for gas circulation or supply and controls the direction and pressure of gas flow.

[0030] Example 4: This example provides a salt-tolerant microbial gradient stress acclimatization device. In addition to the technical solutions of the above examples, it also has the following technical features: the output end of the first air pump 6 is connected to the inside of the first air pipe 8, and the outer surface of the gas mixing box 3 is fixedly connected to the second air pump 9.

[0031] The second air pump 9 is typically used to supply air or oxygen, providing the necessary conditions for the aerobic metabolism of microorganisms.

[0032] Example 5: This example provides a salt-tolerant microbial gradient stress acclimatization device. In addition to the technical solutions of the above examples, it also has the following technical features: a gas dryer 10 is fixedly connected to the outer surface of the second air pump 9, and a second air pipe 13 is fixedly connected to the output end of the second air pump 9.

[0033] Among them, the gas dryer 10 dries the gas to prevent water vapor from interfering with the concentration of the mixed gas or affecting subsequent equipment, and the second gas pipe 13 transports the mixed gas from the gas mixing box 3 to the packing box 5.

[0034] Example 6: This example provides a salt-tolerant microbial gradient stress acclimatization device. In addition to the technical solutions of the above examples, it also has the following technical features: one end of the second air pipe 13 passes through the gas dryer 10 and extends into the interior of the packing box 5, and the outer surface of the gas mixing box 3 is fixedly connected to the injection pipe 11.

[0035] The injection pipe 11 is used to inject specific gases, vapors or liquids into the gas mixing box 3 to adjust the gas phase composition.

[0036] Example 7: This example provides a salt-tolerant microbial gradient stress acclimatization device. In addition to the technical solutions of the above examples, it also has the following technical features: a valve 12 is fixedly installed on the outer surface of the injection pipe 11, and a rotor flow meter 15 is fixedly connected to the outer surface of the first air pipe 8.

[0037] The valve 12 is installed on the injection pipe 11 to precisely control the flow rate or on / off state of the injected substance, and the rotor flow meter 15 can visually display and monitor the instantaneous flow rate of the gas in the first gas pipe 8, which is convenient for precise control.

[0038] Working principle: First, a culture medium containing specific organic pollutants and initial low concentration of salt is prepared in the nutrient tank 1. The water pump 16 is started to pump the culture medium into the packing box 5, so that the suspended packing in it is fully fluidized. When acclimating the wastewater containing volatile organic compounds, the gas circulation system is turned on. The first air pump 6 extracts the gas containing volatile organic compounds from the top space of the packing box 5 and delivers it to the gas mixing box 3 through the first air pipe 8. The second air pump 9 can inject air or specific gas treated by the gas dryer 10. After mixing as needed in the mixing box, it is returned to the bottom of the packing box 5 through the second air pipe 13.

[0039] The embodiments of this application have been described above with reference to the accompanying drawings. Unless otherwise specified, the embodiments and features in the embodiments of this application can be combined with each other. This application is not limited to the specific embodiments described above. The specific embodiments described above are merely illustrative and not restrictive. Those skilled in the art can make many other forms under the guidance of this application without departing from the spirit and scope of the claims, and all of these forms are within the protection scope of this application.

Claims

1. A salt-tolerant microbial gradient stress acclimatization device, characterized in that, include: The nutrient tank (1), the box body (2) and the gas mixing box (3) are provided. A water pump (16) is fixedly connected inside the nutrient tank (1). A water suction pipe (17) is fixedly connected to the water suction end of the water pump (16). A water outlet pipe (4) is fixedly connected to the water outlet end of the water pump (16). A packing box (5) is fixedly connected inside the box body (2). The water outlet end of the water outlet pipe (4) passes through the nutrient tank (1), the box body (2) and the packing box (5) in sequence and extends into the inside of the packing box (5). Four support legs (14) are fixedly connected to the bottom of the box body (2).

2. The salt-tolerant microbial gradient stress acclimatization device according to claim 1, characterized in that, The gas mixing box (3) is fixedly connected to a first gas pipe (8), and one end of the first gas pipe (8) is fixedly connected to an organic gas generator (7).

3. The salt-tolerant microbial gradient stress acclimatization device according to claim 2, characterized in that, The first air pipe (8) has its output end passing through the organic gas generator (7) and extending to the outside. One end of the first air pipe (8) is fixedly connected to the first air pump (6).

4. The salt-tolerant microbial gradient stress acclimatization device according to claim 3, characterized in that, The output end of the first air pump (6) is connected to the inside of the first air pipe (8), and the outer surface of the gas mixing box (3) is fixedly connected to the second air pump (9).

5. The salt-tolerant microbial gradient stress acclimatization device according to claim 4, characterized in that, A gas dryer (10) is fixedly connected to the outer surface of the second air pump (9), and a second air pipe (13) is fixedly connected to the output end of the second air pump (9).

6. The salt-tolerant microbial gradient stress acclimatization device according to claim 5, characterized in that, One end of the second gas pipe (13) passes through the gas dryer (10) and extends into the interior of the packing box (5), and the outer surface of the gas mixing box (3) is fixedly connected to the injection pipe (11).

7. The salt-tolerant microbial gradient stress acclimatization device according to claim 6, characterized in that, A valve (12) is fixedly installed on the outer surface of the injection pipe (11), and a rotor flow meter (15) is fixedly connected to the outer surface of the first air pipe (8).

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