Counting cell, counting cell supply mechanism and detection device

Abstract

The utility model discloses counting pool, counting pool supply mechanism and detection device, counting pool includes the casing, and the casing has the sample inlet, the exhaust port and the window, and the casing defines the inner chamber, first flow channel and second flow channel, and the inner chamber is used for accommodating sample, and the inner chamber includes first sub inner chamber and second sub inner chamber, and the height of first sub inner chamber is less than the height of second sub inner chamber, and the window is used for imaging mechanism to carry out imaging to the sample in the inner chamber, and the sample inlet is communicated with the inner chamber through first flow channel, and the exhaust port is communicated with the inner chamber through second flow channel, and the casing is divided into at least first area and second area, and the inner chamber and the window are arranged in first area, and the sample inlet, the exhaust port, at least partial first flow channel and at least partial second flow channel are arranged in second area, and first area and second area are arranged side by side to not overlap in the height direction of the inner chamber. The utility model discloses by setting up the sample inlet and the exhaust port in the same area, and the structure of counting pool is more compact, and it is helpful to reduce the volume of counting pool.

Description

Technical Field

[0001] This utility model relates to the field of medical device technology, and in particular to a counting pool, a counting pool supply mechanism, and a detection device. Background Technology

[0002] In fields such as life sciences, medicine and health, food, and environmental protection, it is often necessary to perform image or counting analysis on liquids and their components. This typically involves using a flat, transparent counting cell. After the liquid sample is injected, it undergoes a process (such as natural sedimentation or centrifugation) to allow the particles in the liquid sample to settle to the bottom of the cell, where they are then photographed and counted. The counting cell includes a housing with an inner cavity for containing the sample, and an inlet and an outlet connecting the inner cavity. In related technologies, the inlet and outlet are located on different sides of the inner cavity, for example, distributed along the length of the housing on opposite sides of the inner cavity, increasing the size of the housing in that direction. Utility Model Content

[0003] This invention aims to solve at least one of the technical problems existing in the prior art. To this end, this invention proposes a counting cell that can reduce the volume of the counting cell.

[0004] This utility model also proposes a counting pool supply mechanism and a detection device.

[0005] The counting cell according to the first embodiment of this utility model includes a housing, the housing having a sample inlet, an exhaust outlet, and a viewing window, and the housing defining an inner cavity, a first flow channel, and a second flow channel. The inner cavity is used to accommodate a sample, and the inner cavity includes a first sub-inner cavity and a second sub-inner cavity, the height of the first sub-inner cavity being less than the height of the second sub-inner cavity. The viewing window is used for an imaging mechanism to image the sample in the inner cavity. The sample inlet communicates with the inner cavity through the first flow channel to allow the sample to enter the inner cavity. The exhaust outlet communicates with the inner cavity through the second flow channel to allow gas and the sample to exit the inner cavity. The housing is divided into at least a first region and a second region. The inner cavity and the viewing window are disposed in the first region. The sample inlet, the exhaust port, at least a portion of the first flow channel and at least a portion of the second flow channel are disposed in the second region. The first region and the second region are arranged side by side so as not to overlap in the height direction of the inner cavity.

[0006] The counting cell according to the embodiments of this utility model has at least the following beneficial effects: This embodiment concentrates the sample inlet and exhaust outlet in the same area, making the counting cell structure more compact and helping to reduce the volume of the counting cell.

[0007] In other embodiments of this utility model, one of the first sub-cavity and the second sub-cavity is connected to the sample inlet through the first flow channel, and the other is connected to the exhaust port through the second flow channel, and the first sub-cavity and the second sub-cavity are interconnected. Alternatively, the first sub-cavity is connected to the sample inlet through the first flow channel and to the exhaust port through the second flow channel, and the first sub-cavity and the second sub-cavity are interconnected. Alternatively, the second sub-cavity is connected to the sample inlet through the first flow channel and to the exhaust port through the second flow channel, and the first sub-cavity and the second sub-cavity are interconnected.

[0008] In other embodiments of this utility model, both the first sub-cavity and the second sub-cavity are connected to the inlet through the first flow channel.

[0009] In other embodiments of this utility model, the first sub-cavity and the second sub-cavity are interconnected, and at least one of the first sub-cavity and the second sub-cavity is connected to the exhaust port through the second flow channel; or, the first sub-cavity and the second sub-cavity are mutually separated cavities, and both are connected to the exhaust port through the second flow channel.

[0010] In other embodiments of this utility model, the first sub-cavity is connected to the sample inlet through the first flow channel, and the first sub-cavity is connected to the second sub-cavity, wherein the counting cell includes at least one of the following: The first sub-cavity has a first communication port communicating with the first flow channel. The first sub-cavity is approximately fan-shaped, and the first communication port is located in the central region of the fan-shaped cavity. Alternatively, the first sub-cavity is approximately triangular in shape, and the first communication port is located in the vertex region of the triangle. The first sub-cavity is located at a corner of the inner cavity, or the first sub-cavity is located on one side of the inner cavity and between the two corners of the inner cavity corresponding to the two ends of the side edge, or the first sub-cavity is located on one side of the inner cavity and the two ends of the first sub-cavity extend to the two corners of the inner cavity corresponding to the two ends of the side edge. The inner cavity has a second communication port communicating with the second flow channel. A transition structure is provided between the first sub-inner cavity and the second sub-inner cavity. The transition structure includes a first guide section and a dividing section. The first guide section is disposed away from the second communication port relative to the dividing section. The first guide section includes a first guide surface, which is connected to the first cavity surface of the first sub-inner cavity and the second cavity surface of the second sub-inner cavity, respectively. The dividing section includes a dividing interface, which is connected to the first cavity surface and the second cavity surface, respectively. The angle between the first guide surface and the first cavity surface is greater than the angle between the dividing interface and the first cavity surface, so that the sample in the first sub-inner cavity can be guided by the first guide surface and enter the second sub-inner cavity more easily from the first guide section relative to the dividing section. Preferably, the first sub-inner cavity and the second communication port are disposed on the same side of the inner cavity. The transition structure also... The system includes a second guide section, with the dividing section positioned away from the second communication port relative to the second guide section. The second guide section includes a second guide surface, which is connected to both the first cavity surface and the second cavity surface. The angle between the first guide surface and the first cavity surface is greater than the angle between the second guide surface and the first cavity surface, and the angle between the second guide surface and the first cavity surface is greater than the angle between the dividing section and the first cavity surface. Samples within the first sub-cavity can be guided by the first guide surface and enter the second sub-cavity more easily from the first guide section compared to the dividing section and the second guide section. Preferably, the first guide surface extends to the furthest point of the transition structure from the exhaust port. Preferably, the first sub-cavity and the second communication port are located on different sides of the cavity, and there are two first guide sections, with the dividing section located between the two first guide sections. The second sub-cavity is connected to the exhaust port through the second flow channel; preferably, the first sub-cavity has a first communication port connected to the first flow channel, and the second sub-cavity has a second communication port connected to the second flow channel, with the first communication port and the second communication port located on the same side of the cavity; more preferably, the first communication port and the second communication port are located on the side of the cavity facing the second region.

[0011] In other embodiments of this utility model, the second region is located on one side of the first region, the inner cavity has a side facing the second region, and the axis passing through the midpoint of the side is defined as the central axis of the inner cavity. The counting pool includes one of the following schemes: The central axis passes through the injection port, preferably through the center of the injection port; and / or, the central axis passes through the exhaust port, preferably through the center of the exhaust port. The exhaust port and the sample inlet are respectively located on both sides of the central axis. Preferably, both the exhaust port and the sample inlet are located close to the central axis.

[0012] In other embodiments of this utility model, the bottom surface and top surface of the first sub-cavity are mutually parallel planes, and / or the bottom surface and top surface of the second sub-cavity are mutually parallel planes.

[0013] In other embodiments of this utility model, the bottom surface of the first sub-cavity is parallel to the bottom surface of the second sub-cavity, and the bottom surface of the first sub-cavity is higher than the bottom surface of the second sub-cavity; preferably, the top surface of the first sub-cavity and the top surface of the second sub-cavity are on the same plane. Alternatively, the top surface of the first sub-cavity is parallel to the top surface of the second sub-cavity, and the top surface of the first sub-cavity is lower than the top surface of the second sub-cavity; preferably, the bottom surface of the first sub-cavity and the bottom surface of the second sub-cavity are on the same plane.

[0014] In other embodiments of this utility model, the first flow channel is completely disposed in the second region, and / or the second flow channel is completely disposed in the second region.

[0015] In other embodiments of this utility model, the first sub-cavity is disposed at the corner of the cavity; Alternatively, the second sub-cavity may be arranged around the first sub-cavity; Alternatively, the first sub-cavity is disposed on one side of the cavity and located between the two corners of the cavity corresponding to the two ends of the side edge; Alternatively, the first sub-cavity is disposed on one side of the inner cavity, and the two ends of the first sub-cavity extend to the two corners of the inner cavity corresponding to the two ends of the side edge.

[0016] In other embodiments of this utility model, the second region is disposed on one side of the first region.

[0017] In other embodiments of this utility model, the first sub-cavity has a first communication port communicating with the first flow channel, and the second sub-cavity has a second communication port communicating with the second flow channel; Wherein, the first connecting port and the second connecting port are located on the same side of the inner cavity; preferably, the first connecting port and the second connecting port are located on the side of the inner cavity facing the second region; Alternatively, the first connecting port and the second connecting port are located on different sides of the inner cavity; preferably, the inner cavity is rectangular, and the first connecting port and the second connecting port are distributed along the diagonal direction of the inner cavity.

[0018] In other embodiments of this utility model, the bottom and / or top surfaces of the inner cavity are provided with markings; preferably, the markings are provided at the corners of the inner cavity.

[0019] In other embodiments of the present invention, the top surface of the housing is provided with a first groove, the projection of the window on the top surface is located within the projection of the first groove on the top surface, or coincides with the projection of the first groove, and / or, the bottom surface of the housing is provided with a second groove, the projection of the inner cavity on the bottom surface is located within the projection of the second groove on the bottom surface, or coincides with the projection of the second groove.

[0020] In other embodiments of this utility model, the housing is provided with a first anti-mistake structure, which is configured to cooperate with an external second anti-mistake mechanism to place the counting pool in a set posture; preferably, the anti-mistake structure is provided on the side of the housing, and the anti-mistake structure is offset from the central axis of the side perpendicular to its extension direction, or the anti-mistake structure is provided on both adjacent sides of the housing. More preferably, the foolproof structure is configured as a third groove.

[0021] In other embodiments of this utility model, a positioning structure is provided on the top surface of the housing, and the positioning structure is configured to cooperate with an external positioning mechanism to limit the displacement of the counting pool along a set direction; preferably, the positioning structure is provided on the top surface of the housing; preferably, the positioning structure is configured as a fourth groove.

[0022] The counting pool supply mechanism according to the second embodiment of the present invention includes: The storage compartment is used to store the counting pool. An output mechanism is used to output the counting pool inside the chamber.

[0023] The detection device according to the third embodiment of the present invention includes: The aforementioned counting pool supply mechanism; A sampling mechanism is used to perform a sampling operation on the counting cell output by the counting cell supply mechanism; A centrifugation mechanism is used to centrifuge the sampled counting cell. An imaging mechanism is configured to perform a first imaging operation on a sample in the first sub-cavity through the viewing window to obtain first image information, and / or to perform a second imaging operation on a sample in the second sub-cavity through the viewing window to obtain second image information; A transfer mechanism is used to transfer the counting cell output by the counting cell supply mechanism to the sample dispensing mechanism, the centrifugation mechanism, and the imaging mechanism, respectively. A controller is used to obtain detection results based on the first image information and / or the second image information.

[0024] Additional aspects and advantages of this invention will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention. Attached Figure Description

[0025] The present invention will be further described below with reference to the accompanying drawings and embodiments, wherein: Figure 1 This is a top view of the counting cell in one embodiment of the present invention; Figure 2 for Figure 1 A top view of the internal structure of the middle counting cell is shown. Figure 3 for Figure 1 A cross-sectional view along the AA direction; Figure 4 for Figure 3 Enlarged view of region B in the middle; Figure 5 for Figure 1 A cross-sectional view along the CC direction; Figure 6 for Figure 5 Enlarged schematic diagram of region D in the middle; Figure 7 for Figure 1 A schematic diagram of the decomposed counting cell; Figure 8 for Figure 1 A three-dimensional schematic diagram of the base plate of the middle counting cell; Figure 9 This is a simplified schematic diagram of the counting cell in another embodiment of the present invention; Figure 10 This is a simplified schematic diagram of the counting cell in another embodiment of the present invention; Figure 11 This is a simplified schematic diagram of the counting cell in another embodiment of the present invention; Figure 12 This is a simplified schematic diagram of the counting cell in another embodiment of the present invention; Figure 13 This is a simplified schematic diagram of the counting cell in another embodiment of the present invention; Figure 14 This is a simplified schematic diagram of the counting cell in another embodiment of the present invention; Figure 15 This is a simplified schematic diagram of the counting cell in another embodiment of the present invention; Figure 16 This is a simplified schematic diagram of the counting cell in another embodiment of the present invention; Figure 17 This is a simplified schematic diagram of the counting cell in another embodiment of the present invention; Figure 18 This is a simplified schematic diagram of the counting cell in another embodiment of the present invention.

[0026] Figure label: Counting pool 10; Shell 100, inlet 101, outlet 102, window 103, inner cavity 104, marking 1041, first sub-inner cavity 105, first connecting port 1051, first cavity surface 1052, second sub-inner cavity 106, second connecting port 1061, second cavity surface 1062, first flow channel 107, second flow channel 108, first foolproof structure 109, positioning structure 1010, first area 100a, second area 100b, top cover 110, first groove 111, bottom plate 120, second groove 121; Transition structure 200, dividing section 201, interface 2011, second guide section 202, first guide section 203, first guide surface 2031; Central axis L. Detailed Implementation

[0027] The embodiments of this utility model are described in detail below. Examples of these embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain this utility model, and should not be construed as limiting this utility model.

[0028] In the description of this utility model, it should be understood that the directional descriptions, such as up, down, front, back, left, right, etc., indicate the directional or positional relationship based on the directional or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

[0029] In the description of this utility model, "multiple" means two or more; "greater than," "less than," and "exceeding" are understood to exclude the stated number; "above," "below," and "within" are understood to include the stated number. The use of "first" and "second" in the description is merely for distinguishing technical features and should not be construed as indicating or implying relative importance, or implicitly specifying the number of indicated technical features or their sequential relationship.

[0030] In the description of this utility model, unless otherwise explicitly defined, terms such as "setting," "installation," and "connection" should be interpreted broadly, and those skilled in the art can reasonably determine the specific meaning of the above terms in this utility model in conjunction with the specific content of the technical solution.

[0031] In the description of this utility model, the terms "one embodiment," "some embodiments," "illustrative embodiment," "example," "specific example," or "some examples," etc., refer to specific features, structures, materials, or characteristics described in connection with that embodiment or example, which are included in at least one embodiment or example of this utility model. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0032] As mentioned earlier, in related technologies, the inlet and outlet of the counting cell are usually located at opposite ends of the detection area. This necessitates extending a section at each end of the detection area to accommodate the inlet and outlet, increasing the size of the counting cell in the designated direction and consequently increasing the size of related structures on the analytical equipment. Therefore, this invention proposes a counting cell that reduces volume, which will be described below with reference to the accompanying drawings and specific embodiments.

[0033] Reference Figures 1 to 7 The diagram shows a top view of the counting cell 10 in the first embodiment of the present invention, a top view showing the internal structure of the display portion, and a top view along the [unclear text - possibly a diagram or diagram]. Figure 1 Sectional view of section AA, enlarged partial view of region C in the sectional view, along Figure 1 The figure shows a cross-sectional view of section CC, a partially enlarged schematic diagram of region D in the cross-sectional view, and an exploded view. As shown in the figure, the counting cell 10 includes a housing 100, which has a sample inlet 101, an exhaust port 102, and a viewing window 103. The housing 100 defines an inner cavity 104, which is used to contain samples such as urine. The viewing window 103 is used for imaging the sample in the inner cavity 104 by an imaging mechanism. The sample inlet 101 is connected to the inner cavity 104 to allow the sample to enter the inner cavity 104, and the exhaust port 102 is connected to the inner cavity 104 to allow gas and sample to exit the inner cavity 104.

[0034] Reference Figure 3 and Figure 7In some specific embodiments, the housing 100 adopts a split structure. For example, the housing 100 includes an upper cover 110 and a bottom plate 120. When the counting cell 10 is in normal use, the upper cover 110 is disposed on the upper side of the bottom plate 120, and the two are connected by means such as ultrasonic welding. Based on this, the inner cavity 104 is defined by the upper cover 110 and the bottom plate 120. In some specific embodiments, the upper cover 110 is provided with a groove, and the bottom plate 120 is sealed within the groove, thereby forming the inner cavity 104 through the groove on the upper cover 110. In other specific embodiments, the bottom plate 120 is provided with a groove, and the upper cover 110 is sealed within the groove, thereby forming the inner cavity 104 through the groove on the bottom plate 120. In still other specific embodiments, both the upper cover 110 and the bottom plate 120 are provided with grooves, and the grooves of the two together form the inner cavity 104.

[0035] To facilitate sample addition and venting, the sample inlet 101 and the inner cavity 104 can be disposed on the upper cover 110. For example, both are disposed on the upper surface of the upper cover 110, thereby facilitating sample addition by a sample addition component, such as a sample addition needle, from the upper side of the sample inlet 101, and sample extraction by a sample removal component, such as a sample extraction needle, from the venting port 102. Furthermore, to enable the imaging mechanism to acquire an image of the sample in the inner cavity, the housing 100 is at least partially translucent in the viewing window 103. For example, the entire housing 100 is a transparent structure, so that when the imaging mechanism images through the viewing window 103, a light source can illuminate the inner cavity from the opposite side of the viewing window 103, thereby facilitating the formation of a clear image. In some specific embodiments, the viewing window 103 is located on the lower side of the housing 100, for example, on the lower side of the base plate 120; in other specific embodiments, the viewing window 103 is located on the upper side of the housing 100, for example, on the upper side of the upper cover 110. It should be noted that the housing 100 typically has a clear boundary line to mark the extent of the viewing window 103, such as the first groove 111 which will be mentioned in later embodiments. In some specific embodiments, the extent of the viewing window 103 may coincide with the extent of the inner cavity 104, or it may be smaller than the extent of the inner cavity 104.

[0036] Reference Figures 1 to 4The inner cavity 104 includes a first sub-inner cavity 105 and a second sub-inner cavity 106. The height of the first sub-inner cavity 105 is smaller than the height of the second sub-inner cavity 106 (for ease of understanding, these are referred to as the thin region and the thick region, respectively). Thus, the lower height of the first sub-inner cavity 105 results in a smaller volume of liquid per unit area, leading to a smaller total number of particles to be analyzed within the liquid, which may result in missed detections. However, the particles are less likely to stack, facilitating accurate counting. Conversely, the higher height of the second sub-inner cavity 106 allows for a larger volume of liquid per unit area, thus increasing the total number of particles to be analyzed within the liquid. The large total number of particles analyzed can reduce the false negative rate. However, when the particle concentration is high, they tend to overlap, which can affect the accuracy of counting. In this embodiment, by setting cavities with different heights, it is possible to select to image the sample in the first sub-cavity 105 and obtain the analysis result based on the image information, or to select to image the sample in the second sub-cavity 106 and obtain the analysis result based on the image information, or to select to image the samples in the first sub-cavity 105 and the samples in the second sub-cavity 106 separately and obtain the analysis result by combining the image information from both. In some specific embodiments, the height of the first sub-cavity 105 is no greater than 1 mm, and further, the height of the first sub-cavity 105 is no greater than 0.5 mm.

[0037] In this embodiment, refer to Figure 2 The shell 100 is at least divided into a first region 100a and a second region 100b (for ease of understanding, Figure 2 In the diagram, the double-dotted line roughly indicates the boundary between the two regions. Region 100a and region 100b are arranged side-by-side so that they do not overlap in the height direction of the inner cavity 104. For example, when the counting cell 10 is in normal use, region 100a and region 100b are arranged side-by-side horizontally. The inner cavity 104 and the viewing window 103 are located in region 100a, while the sample inlet 101 and the exhaust port 102 are located in region 100b. Since the sample inlet 101, the exhaust port 102, and the inner cavity 104 are located in different regions, refer to... Figure 2 , Figure 8Therefore, the housing 100 also forms a first flow channel 107 and a second flow channel 108, and at least a portion of the first flow channel 107 and at least a portion of the second flow channel 108 are located within the second region 100b, so that the sample inlet 101 can communicate with the inner cavity 104 through the first flow channel 107, and the exhaust port 102 can communicate with the inner cavity 104 through the second flow channel 108. Correspondingly, the first sub-inner cavity 105 has a first connecting port 1051 leading to the sample inlet 101, and the second sub-inner cavity 106 has a second connecting port 1061 leading to the exhaust port 102. In this embodiment, by concentrating the sample inlet 101 and the exhaust port 102 in the same region, compared with the scheme of setting the sample inlet 101 and the exhaust port 102 on opposite sides of the detection region, the structure of the counting cell is more compact, which helps to reduce the volume of the counting cell.

[0038] It should be noted that in some embodiments, there are relatively clear boundaries on the counting pool to distinguish the first region 100a from the second region 100b, for example, using Figure 2 The lower side of the inner cavity 104 serves as the dividing line; in other embodiments, there is no obvious boundary on the counting cell to distinguish the first region 100a and the second region 100b. In this case, one method to distinguish the two regions is to find at least one trajectory on the counting cell 10 that does not pass through the inner cavity 104. When the counting cell 10 is cut along the thickness direction along this trajectory, the counting cell can be divided into two structures, one of which has a complete inner cavity 104, and the other structure has an inlet 101 and an outlet 102.

[0039] Based on the first embodiment, in some embodiments of this utility model, reference is made to Figure 1 , Figure 2 One of the first sub-cavity 105 and the second sub-cavity 106 is connected to the sample inlet 101 via the first flow channel 107, and the other is connected to the exhaust port 102 via the second flow channel 108. The first sub-cavity 105 and the second sub-cavity 106 are interconnected; that is, in this embodiment, the first sub-cavity 105 and the second sub-cavity 106 are connected in series. The sample always flows from one sub-cavity to the other. Depending on the cavity connected to the sample inlet 101, it can be further distinguished as "thin-area injection, thick-area extraction" and "thick-area injection, thin-area extraction." It should be noted that when using the series scheme, the sample inlet 101 is only connected to one of the sub-cavities, thus reducing the control difficulty during liquid injection.

[0040] Based on the first embodiment, in some embodiments of this utility model, reference is made to Figure 9Both the first sub-cavity 105 and the second sub-cavity 106 are connected to the sample inlet 101 via the first flow channel 107. That is, in this embodiment, the first sub-cavity 105 and the second sub-cavity 106 are connected in parallel, and the sample will flow in two parts within the two sub-cavities respectively. In some specific embodiments, there is one sample inlet 101, which is connected to the first sub-cavity 105 and the second sub-cavity 106 via two first flow channels 107 respectively. In other specific embodiments, there are two sample inlets 101, which are connected to the first sub-cavity 105 and the second sub-cavity 106 via two first flow channels 107 respectively. Furthermore, the first sub-cavity 105 and the second sub-cavity 106 can be interconnected, meaning that sample flow can occur between the two sub-cavities. At least one of the first sub-cavity 105 and the second sub-cavity 106 is connected to the exhaust port 102 via the second flow channel 108. In other embodiments, the first sub-cavity 105 and the second sub-cavity 106 are mutually separated cavities, meaning there is no direct sample flow between the two sub-cavities. They are each connected to an exhaust port 102 via a second flow channel 108. In this case, there can be one or two exhaust ports 102. When the first sub-cavity 105 and the second sub-cavity 106 are configured as mutually separated cavities, the samples within the two sub-cavities will not interfere with each other, reducing the generation of air bubbles.

[0041] Based on the first embodiment, in some embodiments of this utility model, the first sub-cavity 105 is connected to the sample inlet 101 through the first flow channel 107 and to the exhaust port 102 through the second flow channel 108. The first sub-cavity 105 and the second sub-cavity 106 are also interconnected. In this case, the sample enters from and exits from the first sub-cavity 105, and the sample also enters the second sub-cavity 106 from the first sub-cavity 105. For example… Figure 10 As shown.

[0042] Based on the first embodiment, in some embodiments of this utility model, the second sub-cavity 106 is connected to the sample inlet 101 through the first flow channel 107 and to the exhaust port 102 through the second flow channel 108. The first sub-cavity 105 and the second sub-cavity 106 are interconnected. In this case, the sample enters from and exits from the second sub-cavity 106, and the sample also enters the first sub-cavity 105 from the second sub-cavity 106. For example… Figure 11 As shown.

[0043] Based on the first embodiment, in some embodiments of this utility model, the first sub-cavity 105 is connected to the sample inlet 101 through the first flow channel 107, and the first sub-cavity 105 is interconnected with the second sub-cavity 106. Because the first sub-cavity 105 and the second sub-cavity 106 are interconnected, when the sample flows from one sub-cavity to the other, air bubbles may become trapped at certain locations in the cavity 104, affecting subsequent counting. Figure 1 Taking an example, if the positions of the first flow channel 107 and the second flow channel 108 are interchanged, that is, if the "thick area injection, thin area effluent" scheme is adopted, since the height of the second sub-cavity 106 is relatively large, the flow of liquid in the second sub-cavity 106 is mainly affected by the liquid pressure. It will follow the path of least flow resistance between the first flow channel 107 and the second flow channel 108 (e.g., the shortest path, i.e.) Figure 1 The liquid quickly reaches the second flow channel 108 from the lower side of the first sub-cavity 105, thereby establishing a relatively stable flow channel. If air bubbles still exist in other parts of the first sub-cavity 105 and the second sub-cavity 106 at this time (e.g., the upper side of the first sub-cavity 105, the upper side of the second sub-cavity 106, etc.), the air bubbles will be trapped here. In this embodiment, a "thin-area injection" scheme is adopted. Since the height of the first sub-cavity 105 is small, the flow of liquid in the first sub-cavity 105 is mainly affected by capillary action. Unlike the usual situation driven by liquid pressure, when the liquid flows in the first sub-cavity 105, it tends to fill the first sub-cavity 105 first, and then flow from the first sub-cavity 105 to the second sub-cavity 106. Therefore, this embodiment can at least avoid the trapping of air bubbles in the first sub-cavity 105.

[0044] When the first sub-cavity 105 is connected to the sample inlet 101 through the first flow channel 107, as described in some embodiments of this utility model, refer to Figure 2 The first sub-cavity 105 has a first connecting port 1051 communicating with the first flow channel 107. The first sub-cavity 105 is approximately fan-shaped, and the first connecting port 1051 is located in the central region of the fan. Thus, the shape of the first sub-cavity 105 conforms to the sample diffusion trend, making it easier for the first sub-cavity 105 to be filled. It should be noted that the term "approximately fan-shaped" in this embodiment should include standard fan shapes and cases approximating fan shapes. For example, replacing the arc segment of a standard fan shape with a combination of convex line segments formed by multiple straight line segments, or a combination of convex line segments formed by straight line segments and arc segments, or a combination of convex line segments formed by arc segments with different curvatures, should all be included within the term "approximately fan-shaped" in this embodiment. Furthermore, this embodiment does not limit the size of the fan angle; it can be as follows: Figure 2 The angle shown is 90°, but it can be any other angle.

[0045] In other embodiments, the first sub-cavity 105 is approximately triangular in shape, with the first connecting port 1051 located at the vertex of the triangle. This shape aligns with the sample diffusion trend, making the first sub-cavity 105 easier to fill. It should be noted that the term "approximately triangular in shape" in this embodiment should include both standard triangles and near-triangular cases. For example, replacing the straight segments opposite the first connecting port 1051 in a standard triangle with a combination of concave line segments formed by multiple straight segments, or a combination of concave line segments formed by straight segments and arc segments, or a combination of concave line segments formed by arc segments with different curvatures, should all be included within the "approximately triangular in shape" definition in this embodiment.

[0046] When the first sub-cavity 105 is connected to the sample inlet 101 through the first flow channel 107, as described in some embodiments of this utility model, refer to Figure 2 The first sub-cavity 105 is located at the corner of the inner cavity 104, that is, the first sub-cavity 105 extends to the adjacent side of the inner cavity 104. For example, the first sub-cavity 105 is located at the lower left corner of the inner cavity 104, extending to the left and lower sides of the inner cavity 104. Based on this, when the shape of the first sub-cavity 105 is approximately fan-shaped, the first connecting opening 1051 is located in the central region of the fan shape, including any one of the cases where the first connecting opening 1051 is located on an adjacent side of the inner cavity 104. For example... Figure 2 The first connecting port 1051 can be located either at the lower left end of the inner cavity 104 or at the lower left end of the inner cavity 104.

[0047] In other embodiments, reference is made to Figure 12 The first sub-cavity 105 is disposed on one side of the cavity 104 and located between the two corners of the cavity 104 corresponding to the two ends of the side edge. In this embodiment, when the exhaust port 102 is connected to the second sub-cavity 106 through the second flow channel 108, the second connecting port 1061 of the second sub-cavity 106 for connecting with the second flow channel 108 can be disposed on the opposite side of the first connecting port 1051, thereby reducing the path difference of the sample flowing from the left to the second connecting port 1061 and from the right to the second connecting port 1061, which helps to reduce the retention of bubbles.

[0048] In other embodiments, reference is made to Figure 13 The first sub-cavity 105 is disposed on one side of the cavity 104, and the two ends of the first sub-cavity 105 extend to the two corners of the cavity 104 corresponding to the two ends of the side edge. That is, the first sub-cavity 105 and the second sub-cavity 106 are typically arranged side by side. For example, the first sub-cavity 105 is disposed on the left side of the cavity 104, and the second sub-cavity 106 is disposed on the right side of the cavity 104.

[0049] When the first sub-cavity 105 is connected to the sample inlet 101 through the first flow channel 107, as described in some embodiments of this utility model, refer to Figure 2 The second sub-cavity 106 is connected to the exhaust port 102 via the second flow channel 108. That is, the first sub-cavity 105 and the second sub-cavity 106 form a typical series structure. The sample flows from the first sub-cavity 105 into the second sub-cavity 106 and then out of the second sub-cavity 106. In some specific embodiments, the first connecting port 1051 and the second connecting port 1061 are located on the same side of the cavity 104 to facilitate the centralized arrangement of the first flow channel 107 and the second flow channel 108. Further, the first connecting port 1051 and the second connecting port 1061 are located on the side of the cavity 104 facing the second region 100b. For example, refer to... Figure 2 The first connecting port 1051 and the second connecting port 1061 are located on the lower side of the inner cavity 104, so that the first flow channel 107 and the second flow channel 108 can be set in the second region 100b.

[0050] When the first sub-cavity 105 is connected to the sample inlet 101 through the first flow channel 107, as described in some embodiments of this utility model, refer to Figure 8 and combined Figure 2 A transition structure 200 is provided between the first sub-cavity 105 and the second sub-cavity 106. The transition structure 200 includes a first guide section 203 and a boundary section 201. The first guide section 203 is positioned away from the second connecting port 1061 relative to the boundary section 201. It should be noted that this limitation refers to the relative relationship between the first guide section 203 and the boundary section 201, and does not mean that the first guide section 203 is the farthest end of the transition structure 200, or that the boundary section 201 is the closest segment of the transition structure 200. (Refer to...) Figure 6 The first guide section 203 includes a first guide surface 2031, which is connected to the first cavity surface 1052 of the first sub-cavity 105 and the second cavity surface 1062 of the second sub-cavity 106, respectively. Figure 4The dividing segment 201 includes a dividing interface 2011, which is connected to the first cavity surface 1052 and the second cavity surface 1062 respectively. It should be noted that the first guide surface 2031, the interface 2011, the first cavity surface 1052, and the second cavity surface 1062 are located on the same side of the inner cavity. For example, if the first guide section 203 and the interface section 201 are located at the bottom of the inner cavity, then the first guide surface 2031 is the upper surface of the first guide section 203, the interface 2011 is a vertical surface, the first cavity surface 1052 is the bottom surface of the first sub-inner cavity 105, and the second cavity surface 1062 is the bottom surface of the second sub-inner cavity 106. If the first guide section 203 and the interface section 201 are located at the top of the inner cavity, then the first guide surface 2031 is the lower surface of the first guide section 203, the interface 2011 is a vertical surface, the first cavity surface 1052 is the top surface of the first sub-inner cavity 105, and the second cavity surface 1062 is the top surface of the second sub-inner cavity 106.

[0051] Among them, reference Figure 4 and Figure 6 The angle β between the first guide surface 2031 and the first cavity surface 1052 is greater than the angle α between the interface 2011 and the first cavity surface 1052. That is, the first guide surface 2031 is gentler than the interface 2011, so that the sample in the first sub-cavity 105 can be guided by the first guide surface 2031 and enter the second sub-cavity 106 more easily from the first guide section 203 than the boundary section 201. As mentioned earlier, the flow of liquid in the second sub-cavity 106 is mainly affected by liquid pressure and tends to flow along the path of least flow resistance. Since the flow distance is positively correlated with the flow resistance, if the sample first enters the second sub-cavity 106 from the boundary segment 201, which is closer to the second connection port 1061, a flow channel will be established along the closer path, and subsequent samples will generally follow this channel, resulting in the inability of gas in other parts to expel trapped bubbles in time. On the other hand, as mentioned earlier, the liquid in the first sub-cavity 105 flows according to the capillary principle, so it is easier to fill the first sub-cavity 105. Once the sample fills the first sub-cavity 105, if there is no interference, it may break through from any position at the junction of the first sub-cavity 105 and the second sub-cavity 106. If it enters from the boundary segment 201, which is closer to the second connection port 1061, bubbles may be trapped due to the aforementioned reasons. Based on this, in this embodiment, a first guide section 203 is provided in the transition structure 200 between the first sub-cavity 105 and the second sub-cavity 106, making it easier for the sample to break through from a random position in the transition structure instead of from breaking through the specific first guide section 203. Since the first guide section 203 is located relatively far from the boundary section 201 and away from the second connecting port 1061, the sample will first fill the area further away from the second connecting port 1061, thereby improving the problem of air bubbles easily getting trapped. Figure 2For example, the flow direction of the sample is indicated by the curved arrows in the figure. The topmost and middle arrows represent the samples flowing from the first guide section 203 and the dividing section 201 to the second sub-cavity 106, respectively. If the sample breaks through the dividing section 201 first, it will tend to flow from the lower side of the cavity 104 to the second connecting port 1061. At this time, the upper side of the cavity 104 (especially the two corners of the upper side) is prone to retaining air bubbles. However, if the sample breaks through the first guide section 203 first, it will fill the upper area of ​​the cavity 104 first, and then flow to the second connecting port 1061, which facilitates the discharge of gas.

[0052] It should be noted that the angle β between the first guide surface 2031 and the first cavity surface 1052 is less than the angle α between the interface 2011 and the first cavity surface 1052. Specifically, this means that at least the angle β between the connection point of the first guide surface 2031 and the first cavity surface 1052 and the first cavity surface 1052 is greater than the angle α between the connection point of the interface 2011 and the first cavity surface 1052 and the first cavity surface 1052. Taking the first guide surface 2031 and the first cavity surface 1052 as an example, if there is a certain angle between them... There is a clearly distinguishable boundary between the first cavity surface 1052 and the first guide surface 2031. For example, if the first cavity surface 1052 is a plane parallel to the horizontal plane, and the first guide surface 2031 is an inclined plane, then the angle β between the first guide surface 2031 and the first cavity surface 1052 is the inclination angle of the inclined plane. Alternatively, if the first cavity surface 1052 is a plane parallel to the horizontal plane, and the first guide surface 2031 is a curved surface, then the angle β between the first guide surface 2031 and the first cavity surface 1052 is the inclination angle of the tangent at the junction of the first guide surface 2031 and the first cavity surface 1052. If there is no clearly distinguishable boundary between the first guide surface 2031 and the first cavity surface 1052, for example, if the first guide surface 2031 and the first cavity surface 1052 form a continuous inclined plane or curved surface, then the angle between the first guide surface 2031 and the first cavity surface 1052 is 0°.

[0053] It should also be noted that the first guide surface 2031 in this invention can be a continuous surface or it can be composed of multiple surfaces connected sequentially along the direction from the first sub-cavity 105 to the second sub-cavity 106. For the latter, for example, the first guide surface 2031 includes inclined surfaces with different degrees of inclination, or it can include a combination of inclined surfaces or curved surfaces.

[0054] When the transition structure 200 includes a first guide section 203 and a boundary section 201, in some embodiments of the present invention, refer to Figure 2 , Figure 8The first sub-cavity 105 and the second connecting port 1061 are located on the same side of the cavity 104. In this case, the transition structure 200 also includes a second guide section 202. The dividing section 201 is located away from the second connecting port 1061 relative to the second guide section 202. That is, it can be considered that the first guide section 203, the dividing section 201, and the second guide section 202 are distributed along a direction away from the second connecting port 1061 to close to the second connecting port 1061. Based on this, this embodiment also ensures that the sample is in the first guide section 203. The flow between the first guide section 203 and the boundary section 201 is more easily broken through, without restricting the order of easier breakthrough between the first guide section 203 and the second guide section 202. This is because even if the sample breaks through the second guide section 202 first, it will stop at the second connecting port 1061 due to surface tension and other factors, and will not flow directly into the second connecting port 1061 until samples from other directions also flow into the second connecting port 1061. In summary, this embodiment can be roughly summarized as follows: among the three sections distributed along the direction from away from the second connecting port 1061 to near the second connecting port 1061 on the transition structure 200, it is sufficient to ensure that samples in the relatively far sections are more easily broken through by samples in the middle sections. It should be noted that this embodiment does not limit the specific range of the first guide section 203, the boundary section 201, and the second guide section 202.

[0055] When the transition structure 200 includes a first guide section 203 and a boundary section 201, in some embodiments of the present invention, referring to... Figure 8 The first guiding surface 2031 is an inclined surface and a curved surface. Taking the inclined surface as an example, the two ends of the inclined surface extend to the top surfaces of the first sub-cavity 105 and the second sub-cavity 106, respectively, or the two ends of the inclined surface extend to the bottom surfaces of the first sub-cavity 105 and the second sub-cavity 106, respectively. The interface 2011 is a vertical surface, and the two ends of the vertical surface extend to the top surfaces of the first sub-cavity 105 and the second sub-cavity 106, respectively, or the two ends of the vertical surface extend to the bottom surfaces of the first sub-cavity 105 and the second sub-cavity 106, respectively. It can be understood that when the sample fills the first sub-cavity 105 and remains in the transition structure 200 between the two sub-cavities, the constraint force of the inclined surface and the curved surface on the liquid is less than the constraint force of the vertical surface on the liquid. Therefore, the liquid at the first guiding section 203 is more likely to overcome its surface tension and flow into the second sub-cavity 106.

[0056] In other embodiments, both the first guide surface 2031 and the interface 2011 are inclined surfaces, and the angle β between the inclined surface of the first guide section 203 and the first cavity surface 1052 is greater than the angle α between the inclined surface of the interface section 201 and the first cavity surface 1052. It is understood that the gentler the slope, the weaker the constraint force on the liquid, and therefore the liquid at the first guide section 203 is more likely to overcome its surface tension and flow into the second sub-cavity 106.

[0057] In other embodiments, both the first guide surface 2031 and the interface 2011 are curved surfaces, and the curvature of the surface of the first guide section 203 is less than the curvature of the surface of the interface section 201, that is, the curvature of the surface of the first guide section 203 is smaller. It is understood that the gentler the surface, the less constraint it exerts on the liquid, and therefore the liquid at the first guide section 203 is more likely to overcome its surface tension and flow into the second sub-cavity 106.

[0058] When the transition structure 200 includes a first guide section 203, a dividing section 201, and a second guide section 202, in some embodiments of this utility model, the second guide section 202 includes a second guide surface, which is connected to the first cavity surface 1052 and the second cavity surface 1062 respectively. The angle between the second guide surface and the first cavity surface 1052 is greater than the angle α between the dividing section 2011 and the first cavity surface 1052, so that the sample in the first sub-cavity 105 can more easily enter the second sub-cavity 106 from the second guide section 202 relative to the dividing section 201. That is, this embodiment can be roughly summarized as follows: among the three sections distributed along the direction away from the second connecting port 1061 to near the second connecting port 1061 on the transition structure 200, it is ensured that the sample in the relatively close section is more easily broken through by the sample in the middle section, thus further reducing the probability of bubble retention. Figure 2 For example, if the sample in the dividing section 201 is easier for the sample in the second guiding section 202 to break through, the sample will flow from the dividing section 201 to the second connecting port 1061 along a roughly arc-shaped trajectory. At this time, air bubbles may be trapped on the right side of the first sub-cavity 105. If the sample in the second guiding section 202 is easier for the sample in the dividing section 201 to break through, the sample can first fill the lower part of the second sub-cavity 106, thereby avoiding the trapping of air bubbles on the right side of the first sub-cavity 105.

[0059] To facilitate the breakthrough of the boundary section 201 in the second guide section 202, in some specific embodiments, the second guide surface is an inclined surface and a curved surface, and the boundary section 2011 is a vertical surface. The constraint force of the inclined surface and the curved surface on the liquid is less than that of the vertical surface on the liquid. Therefore, the liquid at the second guide section 202 can more easily overcome its surface tension and flow into the second sub-cavity 106.

[0060] In other embodiments, both the interface 2011 and the second guide surface are inclined planes, and the angle between the inclined plane of the second guide section 202 and the first cavity surface 1052 is greater than the angle α between the inclined plane of the interface section 201 and the horizontal plane. It is understood that the gentler the slope, the weaker the constraint force on the liquid, and therefore the liquid at the second guide section 202 is more likely to overcome its surface tension and flow into the second sub-cavity 106.

[0061] In other embodiments, both the dividing section 201 and the second guide section 202 have curved surfaces, and the curvature of the second guide surface is less than that of the dividing section 2011, that is, the curvature of the second guide surface is smaller. It is understood that the gentler the curvature, the less constraint it exerts on the liquid, and therefore the liquid at the second guide section 202 is more likely to overcome its surface tension and flow into the second sub-cavity 106.

[0062] When the transition structure 200 includes a first guide section 203, a boundary section 201, and a second guide section 202, in some embodiments of this utility model, the included angle β between the first guide surface 2031 and the first cavity surface 1052 is greater than the included angle between the second guide surface and the first cavity surface 1052, and the included angle between the second guide surface and the first cavity surface 1052 is greater than the included angle α between the boundary 2011 and the first cavity surface 1052, so that the sample in the first sub-cavity 105 can be guided by the first guide surface 2031. The guide is more likely to enter the second sub-cavity 106 from the first guide section 203 than from the boundary section 201 and the second guide section 202. That is, this embodiment can be roughly summarized as follows: among the three sections distributed on the transition structure 200 along the direction away from the second connection port 1061 to near the second connection port 1061, the sample in the relatively far section is the easiest to break through, the sample in the relatively near section breaks through first, and the sample in the middle section breaks through last. In this way, it can be further ensured that the area relatively far from the second connection port 1061 is filled first.

[0063] When the transition structure 200 includes a first guide section 203, a boundary section 201, and a second guide section 202, in some embodiments of the present invention, refer to Figure 2 The axis of the second connecting port 1061 intersects the side of the inner cavity 104 where the first sub-inner cavity 105 and the second connecting port 1061 are provided. This creates an angle between the side and the wall of the second connecting port 1061, increasing the constraint on the sample and making it easier to stop the sample flowing from the second guide section 202 to the second connecting port 1061, allowing for samples from other directions to be collected. For example, the axis of the second connecting port 1061 intersects the lower side of the inner cavity 104. In some specific embodiments, the axis of the second connecting port 1061 is perpendicular to the inner cavity 104 where the first sub-inner cavity 105 and the side of the second connecting port 1061 are provided.

[0064] When the transition structure 200 includes a first guide section 203, a boundary section 201, and a second guide section 202, refer to Figure 2The first guide surface 2031 extends to the farthest end of the transition structure 200 from the exhaust port 102. This further ensures that the area furthest from the second connecting port 1061 is more likely to be filled in the middle area first. For example, when the first sub-cavity 105 is located at the lower left corner of the cavity 104, the first guide section 203 extends to the left side of the cavity 104.

[0065] In other embodiments, reference is made to Figure 2 The second guide surface extends to the end of the transition structure 200 closest to the exhaust port 102. This further ensures that the area closest to the second connecting port 1061 is more likely to be filled in the middle area first. For example, when the first sub-cavity 105 is located at the lower left corner of the cavity 104, the second guide section 202 extends to the lower side of the cavity 104.

[0066] Based on the foregoing embodiments where the first sub-cavity 105 and the second communication port 1061 are located on the same side of the cavity 104, in other embodiments, referring to... Figure 14 The first sub-cavity 105 and the second connecting port 1061 can also be located on different sides of the cavity 104. In this case, there are two first guide sections 203, and the dividing section 210 is located between the two first guide sections 203. For example, the dividing line is the shortest line between the central axis of the transition structure 200 and the second connecting port 1061 (shown as a dashed line in the figure). The transition structure 200 on both sides of the shortest line includes the first guide section 203 and the dividing section 201. That is, when the first sub-cavity 105 and the second connecting port 1061 are on different sides, the second sub-cavity 106 can be divided into two regions. Each part follows the rule that samples from relatively distant sections are easier to break through than samples from relatively nearby sections. In this way, the retention of bubbles can be reduced. Figure 14 Taking the region on the upper left side of the second sub-cavity 106 as an example, if the sample in the dividing section 201 is easier to break through, the sample will more easily establish a flow channel along the direction from the dividing section 201 to the second connecting port 1061, and the upper left corner of the second sub-cavity 106 is prone to retaining air bubbles. If the sample in the first guiding section 203 is easier to break through, the sample will first fill the upper left corner of the second sub-cavity 106, thus reducing the retention of air bubbles.

[0067] When the first sub-cavity 105 and the second connecting port 1061 are located on different sides of the cavity 104, in some embodiments of the present invention, refer to Figure 14 The first sub-cavity 105 and the second connecting port 1061 are roughly diagonally distributed. This can shorten the flow distance difference between the samples flowing out from the first guide section 203 and the samples flowing out from the boundary section 201 and the second connecting port 1061. Combined with the easier breakthrough of the samples in the first guide section 203, the samples flowing out from the first guide section 203 can flow quickly to the second connecting port 1061.

[0068] It should be noted that the above descriptions of the corresponding embodiments of the transition structure 200 are all based on the principle of "thin area sample injection and thick area sample output," but this utility model is not limited to this. Figure 11 As shown in the example, even with the "thin-area injection and thin-area effluent" scheme, the first guide section 203 can still reduce bubble retention. For instance, the sample is introduced from the area located at... Figure 11 When the first guide section 203 of the upper part of the intermediate transition structure 200 is easier to break through, this part of the sample will flow roughly along the upper left, upper right and lower right corners of the second sub-cavity 106 (the flow direction of the sample is roughly indicated by the dashed arrows in the figure), thus making it easier to fill these parts that are prone to stagnating air bubbles.

[0069] Based on the first embodiment, in some embodiments of this utility model, reference is made to Figure 2 The second region 100b is located on one side of the first region 100a. The inner cavity 104 has a side facing the second region 100b. The axis passing through the midpoint of the side is defined as the central axis L of the inner cavity 104. Based on this, this embodiment, by restricting the position of the sample inlet 101 and / or the exhaust port 102 relative to the central axis L, can facilitate the reduction of sample oscillation during subsequent centrifugation. In some specific embodiments, refer to... Figure 2 The exhaust port 102 and the sample inlet 101 are respectively located on both sides of the central axis L. Furthermore, both the exhaust port 102 and the sample inlet 101 are positioned close to the central axis L. It should be noted that, for ease of sample injection, the housing 100 also has a flared opening communicating with the sample inlet 101. This flared opening requires a large area. In this embodiment, by positioning the exhaust port 102 and the sample inlet 101 on both sides of the central axis L, the arrangement satisfies the requirement of being as close to the central axis L as possible, while also making full use of the upper edge of the second region 100b. Figure 2 The space in the left and right directions should be avoided to prevent the increase of the upper edge of the second area 100b. Figure 2 Dimensions in the front-to-back direction.

[0070] In some specific embodiments, the central axis L passes through the injection port 101, and further, the central axis L passes through the center of the injection port 101; in some specific embodiments, the central axis L passes through the exhaust port 102, and further, the central axis L passes through the center of the exhaust port 102. In this embodiment, the centers of the exhaust port 102 and the injection port 101 can be on the same side of the central axis L or on different sides of the central axis L.

[0071] Based on the first embodiment, in some embodiments of this utility model, reference is made to Figure 3 , Figure 4The bottom and top surfaces of the first sub-cavity 105 are parallel planes, meaning the height of the first sub-cavity 105 is constant, which helps reduce molding difficulty. In some specific embodiments, the bottom and top surfaces of the first sub-cavity 105 are both parallel to the horizontal plane. In other embodiments, the bottom and top surfaces of the second sub-cavity 106 are parallel planes, meaning the height of the first sub-cavity 105 is constant, which helps reduce molding difficulty. In some specific embodiments, the bottom and top surfaces of the second sub-cavity 106 are both parallel to the horizontal plane.

[0072] Based on the first embodiment, in some embodiments of this utility model, reference is made to Figure 3 , Figure 4 The top surface of the first sub-cavity 105 is parallel to the top surface of the second sub-cavity 106, and the top surface of the first sub-cavity 105 is lower than the top surface of the second sub-cavity 106, thus making the height of the first sub-cavity 105 less than the height of the second sub-cavity 106. For example, a boss structure facing the bottom plate 120 can be provided on the upper cover 110 at a position corresponding to the first sub-cavity 105. In some specific embodiments, the bottom surface of the first sub-cavity 105 and the bottom surface of the second sub-cavity 106 are on the same plane.

[0073] In other embodiments, the bottom surface of the first sub-cavity 105 is parallel to the bottom surface of the second sub-cavity 106, and the bottom surface of the first sub-cavity 105 is higher than the bottom surface of the second sub-cavity 106, thereby making the height of the first sub-cavity 105 less than the height of the second sub-cavity 106. For example, a boss structure facing the upper cover 110 can be provided on the base plate 120 at a position corresponding to the first sub-cavity 105. In some specific embodiments, the top surface of the first sub-cavity 105 and the top surface of the second sub-cavity 106 are on the same plane.

[0074] Based on the first embodiment, in some embodiments of this utility model, reference is made to Figure 2 The first flow channel 107 is entirely disposed within the second region 100b. In other embodiments, refer to... Figure 2 The second flow channel 108 is entirely located within the second region 100b. This design allows for a more compact structure of the counting cell and simplifies the flow channel setup.

[0075] Based on the first embodiment, in some embodiments of this utility model, reference is made to Figure 15 The first sub-cavity 105 is located at the corner of the inner cavity 104, that is, the first sub-cavity 105 extends to the adjacent side of the inner cavity 104. For example, the first sub-cavity 105 is located at the upper right corner of the inner cavity 104, extending to the upper and right sides of the inner cavity 104. It should be noted that in this embodiment, the first sub-cavity 105 may or may not be connected to the sample inlet 101.

[0076] In other embodiments, reference is made to Figure 16 The second sub-cavity 106 is arranged around the first sub-cavity 105. For example, the cavities 104 and 105 are generally rectangular, and the second sub-cavity 106 is an annular region surrounding the first sub-cavity 105. In this embodiment, both the sample inlet 101 and the exhaust outlet 102 are connected to the second sub-cavity 106.

[0077] In other embodiments, reference is made to Figure 17 The first sub-cavity 105 is disposed on one side of the cavity 104 and is located between the two corners of the cavity 104 corresponding to the two ends of the side edge. It should be noted that in this embodiment, the first sub-cavity 105 may or may not be connected to the sample inlet 101.

[0078] In other embodiments, reference is made to Figure 13 The first sub-cavity 105 is disposed on one side of the inner cavity 104, and both ends of the first sub-cavity 105 extend to the two corners of the inner cavity 104 corresponding to the two ends of the side edge. That is, the first sub-cavity 105 and the second sub-cavity 106 are typically arranged side by side. For example, the first sub-cavity 105 is disposed on the left side of the inner cavity 104, and the second sub-cavity 106 is disposed on the right side of the inner cavity 104. It should be noted that in this embodiment, the first sub-cavity 105 may or may not be connected to the injection port 101.

[0079] Based on the first embodiment, in some embodiments of this utility model, reference is made to Figure 2 The second region 100b is located on one side of the first region 100a. That is, the first region 100a and the second region 100b are arranged in a typical parallel manner, and the shape of the inner cavity 104 is more regular, which is convenient for design and processing.

[0080] Based on the first embodiment, in some embodiments of this utility model, reference is made to Figure 2 The first connecting port 1051 and the second connecting port 1061 are located on the same side of the inner cavity 104 to facilitate the centralized arrangement of the first flow channel 107 and the second flow channel 108. Furthermore, the first connecting port 1051 and the second connecting port 1061 are located on the side of the inner cavity 104 facing the second region 100b, for example, referring to... Figure 2The first connecting port 1051 and the second connecting port 1061 are located on the lower side of the inner cavity 104. In this way, the first flow channel 107 and the second flow channel 108 can be set in the second region 100b. Thus, the sample inlet 101, the exhaust port 102, the first flow channel 107 and the second flow channel 108 are all set in the second region 100b. The first flow channel 107 and the second flow channel 108 do not need to occupy the space of the first region 100a, and the overall structure is more compact. The first flow channel 107 and the second flow channel 108 do not need to occupy the space of the first region 100a, which helps to reduce the volume of the counting cell 10.

[0081] In other embodiments, reference is made to Figure 18 The first connecting port 1051 and the second connecting port 1061 are located on different sides of the inner cavity 104. In this case, the corresponding flow channels need to be adjusted, and part of the flow channels will extend into the first region 100a. Furthermore, the inner cavity 104 is rectangular, and the first connecting port 1051 and the second connecting port 1061 are distributed along the diagonal direction of the inner cavity 104. This shortens the distance difference when flowing from the first connecting port 1051 to the second connecting port 1061 along different paths. For example, the first connecting port 1051 and the second connecting port 1061 are located at the lower left corner and the upper right corner of the inner cavity 104, respectively. If these are replaced with... Figure 2 In one embodiment, a portion of the second flow channel 108 extends into the first region 100a.

[0082] Reference Figure 2 , Figure 7 Based on the first embodiment, in some embodiments of this utility model, the bottom and / or top surface of the inner cavity 104 is provided with a mark 1041. The mark 1041 can be used for focusing by the imaging mechanism. For example, when the imaging mechanism is a microscope, if the mark 1041 is clear enough within the field of view of the microscope, it means that the microscope has been successfully focused. On the other hand, the mark 1041 can also be used for positioning. The actual imaging area of ​​the microscope is a local area within the viewing window 103, and the housing 100 is usually made of transparent material. It is difficult to position directly without the help of a corresponding reference. The mark 1041 in this embodiment can be used as a positioning reference. Specifically, when the mark 1041 appears in the field of view of the microscope, the imaging area can be located by searching for it in the vicinity.

[0083] In some specific embodiments, the area of ​​the second sub-cavity 106 is usually larger than the area of ​​the first sub-cavity 105. Therefore, the positioning difficulty of the shooting area corresponding to the second sub-cavity 106 is also greater than the positioning difficulty of the first sub-cavity 105. Based on this, the identifier 1041 can be set in the second sub-cavity 106.

[0084] In some specific embodiments, the microscope imaging area is usually located in the middle area of ​​the viewing window 103. In order not to affect the microscope imaging, the mark 1041 is set at the corner of the inner cavity 104.

[0085] In some specific embodiments, identifier 1041 can be an array of multiple fine protrusions formed from the bottom and / or top surface of the inner cavity 104.

[0086] Based on the first embodiment, in some embodiments of this utility model, reference is made to Figure 1 The top surface of the housing 100 is provided with a first groove 111. The projection of the window 103 on the top surface is located within the projection of the first groove 111 on the top surface, or coincides with the projection of the first groove 111. In this way, when the counting cells 10 are stacked, the upper surface can be prevented from being scratched and affecting the light transmittance. When the window 103 is set on the top surface, the first groove 111 can also mark the range of the window 103. In addition, the first groove 111 can also reduce the thickness of the upper cell body of the inner cavity 104, thereby increasing the light transmittance.

[0087] In other embodiments, reference is made to Figure 3 The bottom surface of the housing 100 is provided with a second groove 121. The projection of the inner cavity 104 on the bottom surface is located within the projection of the second groove 121 on the bottom surface, or coincides with the projection of the second groove 121. In this way, when the counting cells 10 are stacked or slide on the table, the lower surface can be prevented from being scratched and affecting the light transmittance. When the window 103 is set on the bottom surface, the second groove 121 can also mark the range of the window 103. In addition, the second groove 121 can also reduce the thickness of the lower cell body of the inner cavity 104, thereby increasing the light transmittance.

[0088] Based on the first embodiment, in some embodiments of this utility model, reference is made to Figure 1 , Figure 7 The housing 100 is provided with a first foolproof structure 109, which is configured to cooperate with an external second foolproof mechanism (e.g., a foolproof mechanism on an analyzer) to position the counting cell 10 in a predetermined orientation. In some embodiments, a foolproof structure is provided on the side of the housing 100, offset from the central axis perpendicular to its extension direction, which can reduce the number of foolproof structures. For example... Figure 1 The foolproof structure is located on the lower side of the housing 100 and is offset from the central axis L. It should be noted that the foolproof structure being offset from the central axis perpendicular to the extension direction of this side means that the foolproof structure and the central axis are located on one side of the central axis of this side.

[0089] In some other embodiments, the housing 100 is provided with a foolproof structure on both adjacent sides, which can also play a foolproof role. In this embodiment, it is not restricted that the foolproof structure must be deviated from the central axis of the side perpendicular to its extension direction.

[0090] In the above embodiments, the foolproof structure can be set as a third groove, so as to avoid forming a protruding structure on the housing 100 and facilitate the storage of the counting pool 10.

[0091] Based on the first embodiment, in some embodiments of this utility model, reference is made to Figure 1 , Figure 7 The top surface of the housing 100 is provided with a positioning structure 1010. The positioning structure 1010 is configured to cooperate with an external positioning mechanism to limit the displacement of the counting cell 10 along a set direction. For example, the positioning structure 1010 can cooperate with a robotic arm on the analyzer to limit the displacement of the counting cell 10 along a set direction. Figure 2 The displacement is in the vertical direction. In some specific embodiments, the positioning structure 1010 is disposed on the top surface of the housing 100 to facilitate cooperation with the robotic arm on the analyzer. In some specific embodiments, the positioning structure 1010 is configured as a fourth groove, thus avoiding the formation of a protruding structure on the housing 100 and facilitating the storage of the counting cell 10.

[0092] It should be noted that the first sub-cavity 105 and the second sub-cavity 106 of this utility model are not limited to the situations shown in the above figures. Specifically, except for the height, this utility model does not limit the first sub-cavity 105 and the second sub-cavity 106. The area of ​​the first sub-cavity 105 can be greater than, less than or equal to the area of ​​the second sub-cavity 106. The first sub-cavity 105 and the second sub-cavity 106 can also adopt other shapes besides those shown in the foregoing embodiments. The number of first sub-cavities 105 and second sub-cavities 106 can be one or more, and the number of first sub-cavities 105 and second sub-cavities 106 can be equal or unequal. Multiple first sub-cavities 105 can share a common inlet 101 or an exhaust port 102, and the same applies to the second sub-cavities 106.

[0093] The second embodiment of this utility model proposes a counting pool supply mechanism, which includes a storage chamber and an output mechanism. The storage chamber is used to store the counting pool 10 in the aforementioned embodiment, and the output mechanism is used to output the counting pool from the storage chamber. That is, the counting pool in this embodiment is a disposable counting pool. In some specific embodiments, the storage chamber includes a storage cavity, in which multiple counting pools 10 are stored. The storage chamber also has an output port communicating with the storage cavity. In some specific embodiments, the output mechanism can be a pushing mechanism, which includes a pushing component and a driving device for driving the pushing component. As the pushing component moves, the counting pool 10 in the storage cavity can be output through the output port. It should be noted that the output mechanism is not limited to this; it can also be a robotic arm or other mechanism.

[0094] The third embodiment of this utility model provides a detection device, which includes a sample loading mechanism, a centrifugation mechanism, an imaging mechanism, a transfer mechanism controller, and the aforementioned counting cell supply mechanism. The sample loading mechanism is used to load a sample into the counting cell 10 output by the counting cell supply mechanism; exemplarily, the sample loading mechanism includes a sample loading needle. The centrifugation mechanism is used to centrifuge the sampled counting cell 10, thereby accelerating the precipitation of particles within the sample. The imaging mechanism is used to perform a first imaging operation on the sample in the first sub-cavity 105 through a viewing window 103 to obtain first image information, and / or, to perform a second imaging operation on the sample in the second sub-cavity 106 through the viewing window 103 to obtain second image information. The transfer mechanism is used to sequentially transfer the counting cell 10 output by the counting cell supply mechanism to the sample loading mechanism, the centrifugation mechanism, and the imaging mechanism; exemplarily, the transfer mechanism can be a robotic arm. The controller is used to obtain detection results based on the first image information and / or the second image information.

[0095] The embodiments of the present invention have been described in detail above with reference to the accompanying drawings. However, the present invention is not limited to the above embodiments. Within the scope of knowledge possessed by those skilled in the art, various changes can be made without departing from the spirit of the present invention. Furthermore, the embodiments of the present invention and the features thereof can be combined with each other unless otherwise specified.

Claims

1. A counting cell, characterized in that, The device includes a housing having an inlet, an outlet, and a viewing window. The housing defines an inner cavity, a first flow channel, and a second flow channel. The inner cavity is used to contain a sample and includes a first sub-cavity and a second sub-cavity, the height of the first sub-cavity being less than the height of the second sub-cavity. The viewing window is used for an imaging mechanism to image the sample within the inner cavity. The inlet communicates with the inner cavity through the first flow channel to allow the sample to enter the inner cavity. The outlet communicates with the inner cavity through the second flow channel to allow gas and the sample to exit the inner cavity. The housing is divided into at least a first region and a second region. The inner cavity and the viewing window are disposed in the first region. The sample inlet, the exhaust port, at least a portion of the first flow channel and at least a portion of the second flow channel are disposed in the second region. The first region and the second region are arranged side by side so as not to overlap in the height direction of the inner cavity.

2. The counting cell according to claim 1, characterized in that, One of the first sub-cavity and the second sub-cavity is connected to the sample inlet through the first flow channel, and the other is connected to the exhaust port through the second flow channel, and the first sub-cavity and the second sub-cavity are interconnected; Alternatively, the first sub-cavity is connected to the sample inlet through the first flow channel and to the exhaust port through the second flow channel, and the first sub-cavity and the second sub-cavity are interconnected. Alternatively, the second sub-cavity is connected to the sample inlet through the first flow channel and to the exhaust port through the second flow channel, and the first sub-cavity and the second sub-cavity are interconnected.

3. The counting cell according to claim 1, characterized in that, Both the first sub-cavity and the second sub-cavity are connected to the inlet via the first flow channel.

4. The counting cell according to claim 3, characterized in that, The first sub-cavity and the second sub-cavity are interconnected, and at least one of the first sub-cavity and the second sub-cavity is connected to the exhaust port through the second flow channel; or, the first sub-cavity and the second sub-cavity are mutually separated cavities, and both are connected to the exhaust port through the second flow channel.

5. The counting cell according to claim 1, characterized in that, The first sub-cavity is connected to the sample inlet via the first flow channel, and the first sub-cavity is interconnected with the second sub-cavity, wherein the counting cell includes at least one of the following: The first sub-cavity has a first communication port communicating with the first flow channel. The first sub-cavity is approximately fan-shaped, and the first communication port is located in the central region of the fan-shaped cavity. Alternatively, the first sub-cavity is approximately triangular in shape, and the first communication port is located in the vertex region of the triangle. The first sub-cavity is located at a corner of the inner cavity, or the first sub-cavity is located on one side of the inner cavity and between the two corners of the inner cavity corresponding to the two ends of the side edge, or the first sub-cavity is located on one side of the inner cavity and the two ends of the first sub-cavity extend to the two corners of the inner cavity corresponding to the two ends of the side edge.

6. The counting cell according to claim 1, characterized in that, The first sub-cavity is connected to the sample inlet through the first flow channel, and the first sub-cavity is interconnected with the second sub-cavity. The cavity has a second connection port connected to the second flow channel. A transition structure is provided between the first sub-cavity and the second sub-cavity. The transition structure includes a first guide section and a boundary section. The first guide section is located away from the second connection port relative to the boundary section. The first guide section includes a first guide surface, which is connected to the first cavity surface of the first sub-cavity and the second cavity surface of the second sub-cavity. The boundary section includes an interface, which is connected to the first cavity surface and the second cavity surface. The angle between the first guide surface and the first cavity surface is greater than the angle between the interface and the first cavity surface, so that the sample in the first sub-cavity can be guided by the first guide surface and enter the second sub-cavity more easily from the first guide section relative to the boundary section.

7. The counting cell according to claim 6, characterized in that, The first sub-cavity and the second connecting port are located on the same side of the cavity. The transition structure further includes a second guide section. The dividing section is located away from the second connecting port relative to the second guide section. The second guide section includes a second guide surface, which is connected to the first cavity surface and the second cavity surface respectively. The angle between the first guide surface and the first cavity surface is greater than the angle between the second guide surface and the first cavity surface, and the angle between the second guide surface and the first cavity surface is greater than the angle between the dividing section and the first cavity surface. The sample in the first sub-cavity can be guided by the first guide surface and enter the second sub-cavity more easily from the first guide section than from the dividing section and the second guide section.

8. The counting cell according to claim 6, characterized in that, The first guide surface extends to the farthest end of the transition structure from the exhaust port.

9. The counting cell according to claim 6, characterized in that, The first sub-cavity and the second communication port are located on different sides of the cavity, and there are two first guide sections. The dividing section is located between the two first guide sections.

10. The counting cell according to claim 1, characterized in that, The first sub-cavity is connected to the sample inlet through the first flow channel, and the first sub-cavity is connected to the second sub-cavity. The second sub-cavity is connected to the exhaust port through the second flow channel.

11. The counting cell according to claim 10, characterized in that, The first sub-cavity has a first communication port communicating with the first flow channel, and the second sub-cavity has a second communication port communicating with the second flow channel. The first communication port and the second communication port are located on the same side of the cavities.

12. The counting cell according to claim 11, characterized in that, The first and second connecting ports are located on the side of the inner cavity facing the second region.

13. The counting cell according to claim 1, characterized in that, The second region is located on one side of the first region, and the inner cavity has a side facing the second region. The axis passing through the midpoint of the side is defined as the central axis of the inner cavity, and the central axis passes through the injection port.

14. The counting cell according to claim 13, characterized in that, The central axis passes through the center of the injection port; and / or, the central axis passes through the exhaust port.

15. The counting cell according to claim 13, characterized in that, The central axis passes through the center of the exhaust port.

16. The counting cell according to claim 1, characterized in that, The second region is located on one side of the first region, and the inner cavity has a side facing the second region. The axis passing through the midpoint of the side is defined as the central axis of the inner cavity, and the exhaust port and the sample inlet are respectively located on both sides of the central axis.

17. The counting cell according to claim 16, characterized in that, Both the exhaust port and the sample inlet are located near the central axis.

18. The counting cell according to claim 1, characterized in that, The bottom and top surfaces of the first sub-cavity are parallel planes, and / or the bottom and top surfaces of the second sub-cavity are parallel planes.

19. The counting cell according to claim 1, characterized in that, The bottom surface of the first sub-cavity is parallel to the bottom surface of the second sub-cavity, and the bottom surface of the first sub-cavity is higher than the bottom surface of the second sub-cavity.

20. The counting cell according to claim 19, characterized in that, The top surface of the first sub-cavity and the top surface of the second sub-cavity are on the same plane.

21. The counting cell according to claim 1, characterized in that, The top surface of the first sub-cavity is parallel to the top surface of the second sub-cavity, and the top surface of the first sub-cavity is lower than the top surface of the second sub-cavity.

22. The counting cell according to claim 21, characterized in that, The bottom surface of the first sub-cavity and the bottom surface of the second sub-cavity are on the same plane.

23. The counting cell according to claim 1, characterized in that, The first flow channel is completely disposed within the second region, and / or the second flow channel is completely disposed within the second region.

24. The counting cell according to claim 1, characterized in that, The first sub-cavity is located at the corner of the cavity; Alternatively, the second sub-cavity may be arranged around the first sub-cavity; Alternatively, the first sub-cavity is disposed on one side of the cavity and located between the two corners of the cavity corresponding to the two ends of the side edge; Alternatively, the first sub-cavity is disposed on one side of the inner cavity, and the two ends of the first sub-cavity extend to the two corners of the inner cavity corresponding to the two ends of the side edge.

25. The counting cell according to claim 1, characterized in that, The second region is located on one side of the first region.

26. The counting cell according to claim 1, characterized in that, The first sub-cavity has a first communication port communicating with the first flow channel, and the second sub-cavity has a second communication port communicating with the second flow channel; The first connecting port and the second connecting port are located on the same side of the inner cavity.

27. The counting cell according to claim 26, characterized in that, The first and second connecting ports are located on the side of the inner cavity facing the second region.

28. The counting cell according to claim 1, characterized in that, The first sub-cavity has a first communication port communicating with the first flow channel, and the second sub-cavity has a second communication port communicating with the second flow channel; The first connecting port and the second connecting port are located on different sides of the inner cavity.

29. The counting cell according to claim 28, characterized in that, The inner cavity is rectangular, and the first and second connecting ports are distributed along the diagonal direction of the inner cavity.

30. The counting cell according to claim 1, characterized in that, The bottom and / or top surfaces of the inner cavity are marked.

31. The counting cell according to claim 30, characterized in that, The marking is located at the corner of the inner cavity.

32. The counting cell according to claim 1, characterized in that, The top surface of the housing is provided with a first groove, and the projection of the window on the top surface is located within the projection of the first groove on the top surface, or coincides with the projection of the first groove, and / or the bottom surface of the housing is provided with a second groove, and the projection of the inner cavity on the bottom surface is located within the projection of the second groove on the bottom surface, or coincides with the projection of the second groove.

33. The counting cell according to claim 1, characterized in that, The housing is provided with a first foolproof structure, which is configured to cooperate with an external second foolproof mechanism to place the counting pool in a set posture.

34. The counting cell according to claim 33, characterized in that, The mis-proof structure is provided on the side of the housing, and the mis-proof structure is offset from the central axis of the side perpendicular to its extension direction, or the mis-proof structure is provided on both adjacent sides of the housing.

35. The counting cell according to claim 34, characterized in that, The foolproof structure is configured as a third groove.

36. The counting cell according to claim 1, characterized in that, The top surface of the housing is provided with a positioning structure, which is configured to cooperate with an external positioning mechanism to limit the displacement of the counting cell along a set direction.

37. The counting cell according to claim 36, characterized in that, The positioning structure is disposed on the top surface of the housing.

38. The counting cell according to claim 36, characterized in that, The positioning structure is configured as a fourth groove.

39. A counting pool supply mechanism, characterized in that, include: The storage container is used to store the counting pool as described in any one of claims 1 to 38; An output mechanism is used to output the counting pool inside the chamber.

40. A detection device, characterized in that, include: The counting pool supply mechanism as described in claim 39; A sampling mechanism is used to perform a sampling operation on the counting cell output by the counting cell supply mechanism; A centrifugation mechanism is used to centrifuge the sampled counting cell. An imaging mechanism is configured to perform a first imaging operation on a sample in the first sub-cavity through the viewing window to obtain first image information, and / or to perform a second imaging operation on a sample in the second sub-cavity through the viewing window to obtain second image information; A transfer mechanism is used to transfer the counting cell output by the counting cell supply mechanism to the sample dispensing mechanism, the centrifugation mechanism, and the imaging mechanism, respectively. A controller is used to obtain detection results based on the first image information and / or the second image information.

Images