Portable saliva antioxidant activity colorimetric detection box

Abstract

The utility model discloses portable saliva antioxidant activity colorimetric detection box belongs to biomedical detection technical field, including the casing, the casing is by black polylactic acid material integrated injection molding, and the casing top is equipped with smart mobile phone shooting platform, the smart mobile phone shooting platform includes the positioning recess, and the positioning recess top four sides are equipped with EVA antiskid paste, the utility model discloses a casing top is equipped with mobile phone shooting platform, and places the sample loading system that constitutes with the white polylactic acid sample loading board and a plurality of sample grooves of drawing table, and the optical detection system that the LED lamp strip light source parameter and the white polylactic acid sample loading board top located in the detection position and the shooting window interval is the standard forming distance and constitutes, detects the detection box when using first carries out the calibration and establishes standard curve, and then carries out the detection of the liquid sample of deliquoring, and the detection is quick, and the single detection cost is less than 8 yuan, and the laboratory detection expense reduces 98% than, can be applicable to community hospital saliva antioxidant capacity screening and athlete training load monitoring.

Description

Technical Field

[0001] This utility model relates to the field of biomedical detection technology, and in particular to a portable colorimetric detection kit for saliva antioxidant activity. Background Technology

[0002] Current methods for detecting the antioxidant activity of saliva require large equipment such as UV spectrophotometers, which are complex to operate and cannot be performed rapidly on-site. While microfluidic paper-based chip technology simplifies the detection process, it still presents the following problems: 1. Lack of batch sample parallel processing capability (traditional detection kits only support single samples).

[0003] 2. Ambient light interference causes color difference in mobile phone imaging >15%.

[0004] 3. Specular reflection from the sample carrying system caused image overexposure (tests showed a reflection loss of up to 30%).

[0005] 4. Insufficient standardization: The sample slots lack positioning benchmarks, resulting in a mobile phone recognition accuracy of only 68.5%.

[0006] Therefore, a portable saliva antioxidant activity colorimetric detection kit is proposed to solve the above problems. Utility Model Content

[0007] The purpose of this invention is to address the shortcomings of existing technologies by proposing a portable saliva antioxidant activity colorimetric detection kit.

[0008] To achieve the above objectives, the present invention adopts the following technical solution: A portable saliva antioxidant activity colorimetric test kit includes a housing made of black polylactic acid (PLA) material through integral injection molding. A smartphone camera platform is located at the top of the housing, including a positioning recess with EVA anti-slip pads on all four sides of the top. A camera window is located on one side of the positioning recess, and a smartphone is held inside the positioning recess. The inner walls of the housing are covered with a stone-patterned reflective film. Four circularly arranged LED light strips are located on the inner wall of the top of the housing. A double-track guide groove is located on the inner wall of the bottom of the housing, and a sliding tray is slidably mounted on the top of the double-track guide groove. A white PLA sample holder is located on the top side of the sliding tray near the camera window, and a pull handle is fixedly attached to the outer wall of the sliding tray away from the camera window.

[0009] Preferably, the housing has a USB charging port on the side near the shooting window, the housing length is 180mm, the housing width is 100mm, the housing height is 600mm, the shooting window length is 40mm, and the shooting window width is 30mm.

[0010] Preferably, the light-emitting axis of the LED light strip forms an angle of 45°±2° with the horizontal plane, and the four LED light strips are located at the bottom of the shooting window.

[0011] Preferably, the LED light strip is a cool white light source with a color temperature of 5500K±200K, a color rendering index Ra>95, and an adjustable illuminance range of 3000-8000 lux.

[0012] Preferably, the white polylactic acid (PLA) sample carrier has guide rail sliders fixedly installed at both ends of the bottom end near the imaging window. The center of the white PLA sample carrier has a red crosshair. The white PLA sample carrier has sample slots arranged in a rectangular array, and the bottom of the sample slots is engraved with laser positioning crosshairs. The outer wall of the white PLA sample carrier is frosted, and the roughness Ra of the outer wall of the white PLA sample carrier is 3.2 μm. The distance between the top of the white PLA sample carrier at the detection position and the imaging window is 35 mm ± 1 mm.

[0013] Preferably, the number of sample slots is 12, the depth is 5mm±0.2mm, the center-to-center distance between slots is 12mm, the diameter is 10mm±0.1mm, the width of the red crosshair is 0.3mm, and the width of the laser positioning crosshair is 0.1mm.

[0014] Preferably, the smartphone shooting platform has a tilt angle of 15°, a positioning recess height of 5mm, and an EVA anti-slip sticker with a friction coefficient μ of 0.8.

[0015] The beneficial effects of this utility model are as follows: 1. The portable saliva antioxidant activity colorimetric detection kit proposed in this utility model consists of a mobile phone shooting platform at the top of the shell, a placement platform, a white polylactic acid sample carrier plate, and multiple sample slots forming a sample carrying system. The optical detection system is formed by the LED light strip light source parameters and the standard molding distance between the top of the white polylactic acid sample carrier plate located at the detection position and the shooting window. When using the detection kit, calibration and standard curve establishment are performed first, and then the dehydrated sample is tested. The detection is fast, easy to carry, and the cost of a single test is less than 8 yuan, which is 98% lower than the cost of laboratory testing. It can be used for saliva antioxidant capacity screening in community hospitals and training load monitoring for athletes.

[0016] 2. The portable saliva antioxidant activity colorimetric detection box proposed in this utility model supports batch processing through a multi-sample tank, improving detection efficiency by 300%. The LED ring light source is incident from 45° to diffuse reflection on the frosted surface until vertical imaging. Equipped with LED light strip 301 light source parameters, the color difference is controlled within ΔE<3. The positioning recess, laser positioning crosshair, and red crosshair positioning line constitute triple positioning, making the image distortion rate <1%. The shell is integrally injection molded from black polylactic acid material, and the inner wall of the shell is covered with stone texture embossed reflective film. The detection error is controlled within 5% under 100,000 lux illumination. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the structure of the portable saliva antioxidant activity colorimetric detection kit proposed in this utility model; Figure 2 This is a schematic diagram of the structure of the portable saliva antioxidant activity colorimetric detection box shell proposed in this utility model; Figure 3 This is a top view of the portable saliva antioxidant activity colorimetric detection kit proposed in this utility model. Figure 4 This is a schematic diagram of the structure of the white polylactic acid sample carrier of the portable saliva antioxidant activity colorimetric detection kit proposed in this utility model; Figure 5 This is a side view of the white polylactic acid sample carrier of the portable saliva antioxidant activity colorimetric detection kit proposed in this utility model. Figure 6 This is a top view of the white polylactic acid sample carrier of the portable saliva antioxidant activity colorimetric detection kit proposed in this utility model. Figure 7 This is an enlarged structural schematic diagram of the sample cell of the portable saliva antioxidant activity colorimetric detection kit proposed in this utility model; Figure 8 This is a top view of the shell of the portable saliva antioxidant activity colorimetric detection box proposed in this utility model; Figure 9 This is a bottom view of the imaging window of the portable saliva antioxidant activity colorimetric detection box proposed in this utility model. Figure 10 This is a top view of the placement platform of the portable saliva antioxidant activity colorimetric detection box proposed in this utility model. Figure 11 This is a side view of the placement platform of the portable saliva antioxidant activity colorimetric detection box proposed in this utility model. Figure 12 This is a bottom view of the structure of the portable saliva antioxidant activity colorimetric detection box proposed in this utility model, with the platform in place.

[0018] In the image: 101 Smartphone shooting platform, 102 Positioning recess, 103 EVA anti-slip pad, 104 Shooting window, 105 USB interface, 106 Housing, 107 Smartphone, 108 Dual-rail guide groove; 201 White polylactic acid sample holder, 202 Pull-out handle, 203 Sample slot, 204 Laser positioning crosshair, 205 Red crosshair, 206 Placement platform, 207 Guide rail slider. 301 LED light strip. Detailed Implementation

[0019] Reference Figure 1-12 A portable saliva antioxidant activity colorimetric test kit includes a housing 106, which is integrally injection molded from black polylactic acid. A smartphone camera platform 101 is located at the top of the housing 106. The smartphone camera platform 101 includes a positioning recess 102, and EVA anti-slip pads 103 are provided on the four sides of the top of the positioning recess 102. A shooting window 104 is provided on one side of the positioning recess 102. A smartphone 107 is snapped into the positioning recess 102. The inner wall of the housing 106 is covered with a stone-patterned embossed reflective film. Four ring-shaped LED light strips 301 are provided inside the inner wall of the top of the housing 106. A double-track guide groove 108 is provided on the inner wall of the bottom of the housing 106, and a sliding drawer 206 is slidably installed at the top of the double-track guide groove 108. A white polylactic acid sample carrier 201 is provided on the top side of the drawer 206 near the shooting window 104, and a pull handle 202 is fixedly provided on the outer wall of the drawer 206 away from the shooting window 104.

[0020] In this utility model, the housing 106 is provided with a USB charging interface 105 on the side near the shooting window 104, which can support 5V / 2A input. The housing 106 is 180mm long, 100mm wide, and 600mm high. The shooting window 104 is 40mm long and 30mm wide. The light-emitting axis of the LED light strip 301 forms an angle of 45°±2° with the horizontal plane, and the four LED light strips 301 are located at the bottom of the shooting window 104; LED strip 301 is a cool white light source with a color temperature of 5500K±200K, a color rendering index Ra>95, and an adjustable illuminance range of 3000-8000 lux; A guide rail slider 207 is fixedly installed on both ends of the white polylactic acid sample holder 201 near the imaging window 104. A red cross mark 205 is provided in the center of the white polylactic acid sample holder 201. The white polylactic acid sample holder 201 has sample slots 203 arranged in a rectangular array, and the bottom of the sample slots 203 is engraved with laser positioning cross marks 204. The outer wall of the white polylactic acid sample holder 201 is frosted, and the roughness Ra of the outer wall of the white polylactic acid sample holder 201 is 3.2μm. The distance between the top of the white polylactic acid sample holder 201 at the detection position and the imaging window 104 is 35mm±1mm. The number of sample slots 203 is 12, the depth is 5mm±0.2mm, the center-to-center distance of the slots is 12mm, the diameter is 10mm±0.1mm, the width of the red crosshair 205 is 0.3mm, and the width of the laser positioning crosshair 204 is 0.1mm. The smartphone shooting platform 101 has a tilt angle of 15°, the positioning recess 102 has a height of 5mm, and the EVA anti-slip pad 103 has a friction coefficient μ of 0.8.

[0021] Working principle: The sample carrying system consists of a mobile phone shooting platform 101 at the top of the housing 106, a drawer 206, a white polylactic acid sample carrier 201, and multiple sample slots 203. The light source parameters of the LED light strip 301 and the standard molding distance between the top of the white polylactic acid sample carrier 201 and the shooting window 104 at the detection position constitute the optical detection system. When using the detection box, it is first calibrated and a standard curve is established before the dehydrated sample is detected. The detection is fast, portable, and the cost of a single test is less than 8 yuan, which is 98% lower than the cost of laboratory testing. It can be used for screening saliva antioxidant capacity in community hospitals and monitoring the training load of athletes. The 12-sample-cell 203 supports batch processing, improving detection efficiency by 300%. The LED ring light source is incident from 45° to diffuse reflection on the frosted surface and then to vertical imaging. Equipped with LED light strip 301 light source parameters, the color difference is controlled within ΔE < 3. The positioning recess 102, laser positioning crosshair 204, and red crosshair positioning line 205 constitute triple positioning, making the image distortion rate < 1%. The shell 106 is integrally injection molded from black polylactic acid material, and the inner wall of the shell 106 is covered with stone texture embossed reflective film. Under 100,000 lux illumination, the detection error is controlled within 5%. In use, the smartphone 107 is placed inside the positioning recess 102, with the lens pressed against the shooting window 104. The EVA anti-slip sticker 103 is used for locking and anti-slip. Then, the smartphone 107's APP recognizes the red crosshair 205 to complete XY positioning (accuracy ±0.1mm), and the laser positioning crosshair 204 achieves Z-axis distance measurement (error <0.3mm). Next, the area of ​​the white polylactic acid sample plate 201 is photographed to extract the RGB mean value until the calibration reaches R=G=B=245±3. Then, the paper-based chip pre-loaded with artificial simulated enzyme is placed into the sample slot (203), and 100μL of standard ascorbic acid of different concentrations is added for calibration. The sample was prepared by allowing the solution to stand for 3 minutes to develop color. Then, the LED light source (5000 lux setting) was activated. The APP identified the laser positioning crosshair 204 and analyzed the HSV value of the color development area in sample cell 203 to generate a standard curve. Next, the paper-based chip pre-loaded with artificial enzyme was placed into sample cell (203). At the same time, the saliva was diluted 10 times, and 100 μL of saliva diluted sample was added. The sample was allowed to stand for 3 minutes to develop color, and the LED light source (5000 lux setting) was activated again. The APP identified the laser positioning crosshair 204 and analyzed the HSV value of the color development area in sample cell 203. The antioxidant properties of the saliva were obtained based on the previously generated standard curve.

[0022] The above description is only a preferred embodiment of the present utility model, but the protection scope of the present utility model is not limited thereto. Any equivalent substitutions or changes made by those skilled in the art within the technical scope disclosed in the present utility model, based on the technical solution and the inventive concept of the present utility model, should be included within the protection scope of the present utility model.

Claims

1. A portable colorimetric assay kit for salivary antioxidant activity, comprising a housing (106), characterized in that, The housing (106) is integrally injection molded from black polylactic acid material. A smartphone shooting platform (101) is provided at the top of the housing (106). The smartphone shooting platform (101) includes a positioning recess (102), and EVA anti-slip pads (103) are provided on the four sides of the top of the positioning recess (102). A shooting window (104) is provided on one side of the positioning recess (102). A smartphone (107) is snapped into the inside of the positioning recess (102). The inner wall of the housing (106) is covered with stone pattern embossing. The reflective film has four ring-shaped LED light strips (301) inside the inner wall of the top of the housing (106). The inner wall of the bottom of the housing (106) has a double-track guide groove (108), and a placement platform (206) is slidably installed at the top of the double-track guide groove (108). A white polylactic acid sample plate (201) is provided on the top side of the placement platform (206) near the shooting window (104), and a pull handle (202) is fixedly provided on the outer wall of the side of the placement platform (206) away from the shooting window (104).

2. The portable saliva antioxidant activity colorimetric detection kit according to claim 1, characterized in that, The housing (106) has a USB charging port (105) on the side near the shooting window (104). The housing (106) is 180mm long, 100mm wide, and 600mm high. The shooting window (104) is 40mm long and 30mm wide.

3. The portable saliva antioxidant activity colorimetric detection kit according to claim 1, characterized in that, The light-emitting axis of the LED light strip (301) forms an angle of 45°±2° with the horizontal plane, and the four LED light strips (301) are located at the bottom of the shooting window (104).

4. The portable saliva antioxidant activity colorimetric detection kit according to claim 3, characterized in that, The LED light strip (301) is a cool white light source with a color temperature of 5500K±200K, a color rendering index Ra>95, and an adjustable illuminance range of 3000-8000 lux.

5. The portable saliva antioxidant activity colorimetric detection kit according to claim 1, characterized in that, The white polylactic acid sample holder (201) has guide rail sliders (207) fixedly installed on the bottom two ends near the shooting window (104). The white polylactic acid sample holder (201) has a red cross mark (205) in the center. The white polylactic acid sample holder (201) has sample slots (203) in a rectangular array. The bottom of the sample slots (203) is engraved with laser positioning cross marks (204). The outer wall of the white polylactic acid sample holder (201) is frosted and the roughness Ra of the outer wall of the white polylactic acid sample holder (201) is 3.2μm. The distance between the top of the white polylactic acid sample holder (201) at the detection position and the shooting window (104) is 35mm±1mm.

6. The portable saliva antioxidant activity colorimetric detection kit according to claim 5, characterized in that, The sample grooves (203) are 12 in number, with a depth of 5mm ± 0.2mm, a center-to-center spacing of 12mm, and a diameter of 10mm ± 0.1mm. The red crosshairs (205) have a line width of 0.3mm, and the laser positioning crosshairs (204) have a line width of 0.1mm.

7. The portable saliva antioxidant activity colorimetric detection kit according to claim 1, characterized in that, The smartphone shooting platform (101) has an inclination angle of 15°, a positioning recess (102) height of 5mm, and an EVA anti-slip sticker (103) with a friction coefficient μ of 0.8.

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