An agarose gel electrophoresis assisted positioning device

Abstract

The utility model provides a kind of agarose gel electrophoresis auxiliary positioning device, including electrophoresis tank, glue table, fixed plate, glue plate, position sensor and pilot lamp, glue table and fixed plate are set up in electrophoresis tank left and right interval, the height of fixed plate is same with glue table, fixed plate and glue table can be from left and right sides jointly support glue plate, multiple sample application holes are uniformly spaced and set in the side of glue plate close to glue table along front-back direction, multiple position sensors are installed in the side of glue table close to fixed plate, the number of position sensor and agarose gel sample application hole is same, and position one-to-one correspondence;The detection direction of position sensor keeps consistent with sample application direction, and sample application position is monitored in real time, and one pilot lamp is connected to each position sensor.The structure of the present application compared with existing agarose gel electrophoresis instrument, increase the real-time monitoring to sample application position, ensure the accuracy of sample application operation more simple.

Description

Technical Field

[0001] This application belongs to the field of molecular biology experiments and relates to an agarose gel electrophoresis-assisted positioning device. Background Technology

[0002] Agarose gel electrophoresis is a commonly used instrument for separating and identifying mixtures of DNA and RNA molecules. This electrophoresis instrument uses agarose gel as a support and utilizes the charge effect and molecular sieving effect of DNA molecules during migration to achieve the separation of the mixture. Proteins and nucleic acids carry different charges depending on their pH, resulting in different forces in an electric field and thus different speeds of movement. Based on this principle, they can be separated, and the entire separation process is continuous and uninterrupted. In agarose gel electrophoresis experiments, agarose gel is prepared in a gel casting tank. Then, the sample comb used for gel casting is removed, and the gel and casting tank are placed together in the electrophoresis tank. The electrophoresis buffer in the electrophoresis tank covers the agarose gel, and then a sample gun is used to spot the sample into the wells on the gel. In existing techniques, manual spotting often results in inaccurate positioning of the wells, leading to spotting deviations. Even when the wells are accurately positioned, excessive force may be applied, causing the sample gun tip to penetrate too deeply into the agarose gel, damaging the gel and causing sample leakage. Utility Model Content

[0003] To address the aforementioned problems, this invention proposes an agarose gel electrophoresis-assisted positioning device.

[0004] The technical problem solved by this utility model is achieved through the following technical solution:

[0005] An agarose gel electrophoresis-assisted positioning device includes an electrophoresis tank, a gel placement stage, a fixing plate, a gel support plate, position sensors, and indicator lights. The gel placement stage and the fixing plate are spaced apart on the left and right sides of the electrophoresis tank. The fixing plate is at the same height as the gel placement stage. The fixing plate and the gel placement stage together support the gel support plate from both sides. Multiple sample wells are evenly spaced along the front-back direction on the side of the gel support plate near the gel placement stage. Multiple position sensors are installed on the side of the gel placement stage near the fixing plate. The number of position sensors is the same as the number of agarose gel sample wells, and their positions correspond one-to-one. The detection direction of the position sensors is consistent with the sample placement direction, enabling real-time monitoring of the sample placement position. Each position sensor is electrically connected to an indicator light.

[0006] Furthermore, the indicator light is located at the bottom of the electrophoresis tank below the agarose gel spotting well.

[0007] Furthermore, horizontal sliding grooves are provided on the inner walls of the front and rear sides of the electrophoresis tank. The upper ends of the front and rear sides of the fixing plate are respectively limited and slidably connected to the sliding grooves. The fixing plate can move left and right within the electrophoresis tank along the sliding groove range. Multiple locking holes are evenly spaced below the sliding grooves of the electrophoresis tank. A latch is connected to the front and rear sides of the fixing plate, and a locking rod is connected inside the latch. One end of the locking rod is embedded in the locking hole, and the other end of the locking rod is provided with a locking cover.

[0008] Furthermore, a push rod is fixedly connected to the middle of the fixed plate.

[0009] Furthermore, the latch is a ring structure with internal threads, and the locking rod has external threads. The locking rod and the latch mesh with each other, and rotating the locking rod can control the movement of the locking rod.

[0010] Furthermore, the indicator light can display three different colors.

[0011] Furthermore, the lower part of the adhesive plate is a rectangular flat plate structure, and the front and rear ends of the adhesive plate are respectively connected to limit plates. An agarose gel plate is laid on the upper part of the adhesive plate between the limit plates, and multiple agarose gel spotting holes are evenly spaced along the front and rear direction on one side of the gel placement platform.

[0012] Moreover, the electrophoresis tank is a rectangular box with an opening at the top. Electrodes are provided at the left and right ends of the electrophoresis tank. The gel-placement platform has a boss structure, which can cut off the bottom space inside the electrophoresis tank. The upper part of the gel-placement platform has a planar structure.

[0013] The advantages and positive effects of this utility model are:

[0014] 1. Compared with existing agarose gel electrophoresis apparatus, this device adds real-time monitoring of the sample spotting position to ensure the accuracy of the spotting.

[0015] 2. This device is equipped with a movable fixing plate, which makes operation simpler, avoids waste of gel electrophoresis during operation, ensures accurate detection, can accurately locate the positioning hole, and achieve stable sample spotting. Attached Figure Description

[0016] To more clearly illustrate the technical solution of this utility model, the drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0017] Figure 1 This is a schematic diagram of an agarose gel electrophoresis-assisted positioning device.

[0018] Figure 2 yes Figure 1A schematic diagram showing the connection between the movable fixed plate locking rod and the lock hole.

[0019] Figure 3 This is a schematic diagram of an agarose gel plate.

[0020] The markings in the diagram indicate: 1 is the electrophoresis tank, 2 is the gel placement platform (position sensor), 3 is the indicator light, 4 is the power warning button, 5 is the power indicator light, 6 is the slide, 7 is the lock hole, 8 is the locking rod, 9 is the push rod, 10 is the fixing plate, 11 is the buckle, 12 is the lock cover, 13 is the gel support plate, 14 is the agarose gel, and 15 is the agarose gel spotting well. Detailed Implementation

[0021] To make the structure and advantages of this utility model clearer, the structure of this utility model will be further described below with reference to the accompanying drawings.

[0022] In this embodiment, the vertical direction, horizontal direction, front, back, left, right, etc. are all relative position descriptions and do not limit the actual orientation of the product.

[0023] An agarose gel electrophoresis-assisted positioning device, see attached. Figure 1 As shown, the system includes an electrophoresis tank 1, a glue-laying platform 2, a fixing plate 10, a glue-supporting plate 13, a position sensor, and an indicator light 3. The electrophoresis tank 1 is a rectangular box with an open top. Electrodes are respectively installed at the left and right ends of the electrophoresis tank 1. A power warning button 4 and a power indicator light 5 are installed on the outside of the electrophoresis tank. The glue-laying platform 2 and the fixing plate 10 are arranged at intervals on the left and right sides inside the electrophoresis tank 1. The glue-laying platform 2 is fixedly connected to the electrophoresis tank 1 and is a longitudinally arranged protrusion that can cut off the bottom space inside the electrophoresis tank 1.

[0024] The height of the fixing plate 10 is the same as that of the glue placement platform 2. The fixing plate 10 and the glue placement platform 2 can jointly limit the glue-bearing plate 13 from the left and right sides, keeping the glue-bearing plate 13 in a fixed position.

[0025] On the side of the adhesive plate 13 near the adhesive placement platform 2, a plurality of agarose gel 14 spotting holes 15 are evenly spaced along the front-back direction. On the side of the adhesive placement platform 2 facing the fixing plate 10, a plurality of position sensors are installed. The number of position sensors is the same as the number of agarose gel 14 spotting holes 15, and their positions correspond one-to-one. The detection direction of the position sensors is consistent with the spotting direction, so as to monitor the spotting position in real time.

[0026] An indicator light 3 is installed at the bottom of the electrophoresis tank 1 below the spotting well 15 of the agarose gel 14. The indicator light 3 is perpendicular to the position sensor. The light from the indicator light 3 can pass through the spotting well 15 of the agarose gel 14. The indicator light 3 is connected to the position sensor through a circuit. The color of the indicator light 3 is adjusted in real time according to the detection result of the position sensor. In this embodiment, the indicator light 3 can display three different colors to show the corresponding position information. In this embodiment, the colors are set as follows: orange represents that the spotting tip position is too shallow, green represents that the spotting tip position is moderate, and red represents that the spotting tip position is too deep.

[0027] See appendix Figure 1 , 2 As shown, to ensure support stability for adhesive plates 13 of different sizes, the position of the fixing plate 10 can be adjusted. Specifically, the adjustment structure includes: horizontal grooves 6 are provided on the inner walls of the front and rear sides of the electrophoresis tank 1; limit blocks are provided at the upper ends of the front and rear sides of the fixing plate 10, and these limit blocks are embedded in the grooves 6, allowing the fixing plate 10 to move left and right within the electrophoresis tank 1 along the grooves 6; multiple locking holes 7 are evenly spaced below the grooves 6 of the electrophoresis tank 1; a latch 11 is connected to the front and rear sides of the fixing plate 10, and each latch 11 is connected to a locking rod 8 that can be adjusted in the front-rear direction. One end of the locking rod 8 is embedded in the locking hole 7, and the other end of the locking rod 8 is provided with a locking cover 12 for easy operation. In this embodiment, the latch 11 is a ring structure with internal threads, and the locking rod 8 has external threads. The locking rod 8 meshes with the latch 11, and rotating the locking rod 8 controls its movement.

[0028] For ease of operation, a push rod 9 is fixedly connected to the middle of the fixing plate 10. The position of the fixing plate 10 is moved by the push rod 9 and the sliding groove 6 on the side of the gel electrophoresis tank, and the position of the fixing plate 10 is fixed by the locking rod 8 on the movable fixing plate 10 and the locking hole 7 on the side of the electrophoresis tank 1.

[0029] See appendix Figure 3 As shown, the lower part of the adhesive plate 13 is a rectangular flat plate structure. The front and rear ends of the adhesive plate 13 are respectively connected to the limiting plates. The upper end of the adhesive plate 13 between the limiting plates is covered with an agarose gel 14 plate. Multiple agarose gel 14 sampling holes 15 are evenly spaced along the front and rear direction on one side of the gel placement platform 2.

[0030] This embodiment also includes a power module (not shown in the figure), which is connected to the position sensor and indicator light module 3 and provides them with power. The power module has a power indicator light and a warning button. When the power is insufficient, the indicator light will light up and the warning button will sound a buzzing sound. The warning sound can be turned off manually and the power supply can be replaced.

[0031] The circuit structure uses conventional electrical connection methods for wiring.

[0032] Specific steps for using this device:

[0033] During use, turn on the power, place the electrophoresis solution at both ends of the electrophoresis tank, push the push rod to the appropriate position, and fix the movable fixing plate with the locking rod. Place the agarose gel plate on the substrate, ensuring that the spotting hole position corresponds to the position indicator light position, and then spot the sample.

[0034] The location indicator light can be used to monitor the sampling position in real time based on its color change, thus achieving accurate sampling and avoiding waste and inaccurate detection.

[0035] The above description is merely an embodiment of this utility model and is not intended to limit this utility model. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this utility model should be included within the protection scope of this utility model.

Claims

1. An agarose gel electrophoresis-assisted positioning device, characterized in that... The system includes an electrophoresis tank, a gel placement platform, a fixing plate, a gel support plate, position sensors, and indicator lights. The gel placement platform and fixing plate are spaced apart on the left and right sides of the electrophoresis tank. The fixing plate is the same height as the gel placement platform. The fixing plate and the gel placement platform together support the gel support plate from both sides. Multiple sample wells are evenly spaced along the front-back direction on the side of the gel support plate near the gel placement platform. Multiple position sensors are installed on the side of the gel placement platform near the fixing plate. The number of position sensors is the same as the number of agarose gel sample wells, and their positions correspond one-to-one. The detection direction of the position sensors is consistent with the sample placement direction, allowing for real-time monitoring of the sample placement position. Each position sensor is electrically connected to an indicator light.

2. The agarose gel electrophoresis-assisted positioning device according to claim 1, characterized in that... The indicator light is located at the bottom of the electrophoresis tank below the agarose gel spotting well.

3. The agarose gel electrophoresis-assisted positioning device according to claim 1, characterized in that... The inner walls of the front and rear sides of the electrophoresis tank are provided with horizontal sliding grooves. The upper ends of the front and rear sides of the fixing plate are respectively limited and slidably connected to the sliding grooves. The fixing plate can move left and right within the electrophoresis tank along the sliding groove range. Multiple locking holes are evenly spaced below the sliding grooves of the electrophoresis tank. A latch is connected to the front and rear sides of the fixing plate, and a locking rod is connected inside the latch. One end of the locking rod is embedded in the locking hole, and the other end of the locking rod is provided with a locking cover.

4. The agarose gel electrophoresis-assisted positioning device according to claim 3, characterized in that... A push rod is fixedly connected to the middle of the fixed plate.

5. The agarose gel electrophoresis-assisted positioning device according to claim 3, characterized in that... The latch is a ring structure with internal threads, and the locking rod has external threads. The locking rod and the latch mesh with each other, and rotating the locking rod can control the movement of the locking rod.

6. The agarose gel electrophoresis-assisted positioning device according to claim 1, characterized in that... The indicator light can display three different colors.

7. The agarose gel electrophoresis-assisted positioning device according to claim 1, characterized in that... The lower part of the adhesive plate is a rectangular flat plate structure. The front and rear ends of the adhesive plate are respectively connected to limit plates. An agarose gel plate is laid on the upper part of the adhesive plate between the limit plates. Multiple agarose gel spotting holes are evenly spaced along the front and rear direction on one side of the gel placement platform.

8. The agarose gel electrophoresis-assisted positioning device according to claim 1, characterized in that... The electrophoresis tank is a rectangular box with an opening at the top. Electrodes are provided at the left and right ends of the electrophoresis tank. The gel platform has a boss structure, which can cut off the bottom space inside the electrophoresis tank.

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