Kit for plasma separation from whole blood

Abstract

This invention provides a reagent kit for whole blood plasma separation, comprising a kit body and a top cover. The kit body has a sample chamber, a test strip slot, and a filtration membrane, with the filtration membrane positioned between the sample chamber and the test strip slot. The sample chamber contains a pre-filled target reagent, and the test strip slot is for inserting a test strip. The top cover is sealed and fastened to the opening of the sample chamber. The top cover has a pressing and deforming part; when force is applied to the pressing and deforming part in the direction towards the sample chamber, the plasma from the whole blood sample in the sample chamber can pass through the filtration membrane into the test strip slot to contact the test strip. This invention has a simple and compact structure, is easy and flexible to operate, avoids the use of complex components and requires no additional equipment, significantly reducing manufacturing and purchase costs. It is particularly suitable for home use and facilitates the promotion of POCT technology at the grassroots level.

Description

Technical Field

[0001] This invention belongs to the field of biological detection technology, specifically relating to a reagent kit for whole blood plasma separation. Background Technology

[0002] Currently known methods for separating plasma from whole blood include centrifugation, membrane filtration, and sedimentation. Centrifugation and membrane filtration require machines and can only be performed in fixed locations, making them inflexible and inconvenient. Sedimentation requires whole blood to stand for a long time before plasma can be separated, and it also requires manual extraction and processing of the plasma, resulting in long waiting times and complex operations.

[0003] To overcome the shortcomings of the aforementioned existing methods, related technologies have proposed a method for plasma separation within a paper base. This method facilitates plasma separation, but the separated plasma is stored within the paper base and cannot be collected. There are also methods that use pump pressure to drive a plunger pump, allowing whole blood to pass through a filtration device, thereby achieving plasma separation and collection simultaneously. However, the required equipment and structural components are complex, making it unsuitable for home use and hindering the promotion of POCT (point-of-care testing) technology at the grassroots level. Summary of the Invention

[0004] Therefore, the technical problem to be solved by this utility model is to provide a reagent kit for whole blood plasma separation, which can overcome the shortcomings of existing whole blood plasma separation reagent kits, which require corresponding equipment and are not suitable for home use, thus hindering the promotion of POCT (point-of-care testing) technology at the grassroots level.

[0005] To address the aforementioned problems, this invention provides a whole blood plasma separation kit, comprising a kit body and a top cover. The kit body has a sample chamber, a test strip slot, and a filtration membrane. The filtration membrane is located between the sample chamber and the test strip slot. The sample chamber contains a pre-filled target reagent. The test strip slot is used to insert a test strip for testing. The top cover is sealed and fastened to the opening of the sample chamber. The top cover has a pressing and deforming part. When force is applied to the pressing and deforming part in a direction toward the sample chamber, the plasma from the whole blood sample in the sample chamber can enter the test strip slot through the filtration membrane to contact the test strip for testing.

[0006] In some embodiments, the sample chamber and the test strip slot are connected by a flow channel.

[0007] In some embodiments, the flow channel is provided with a plurality of micropillars spaced apart.

[0008] In some embodiments, the reagent kit body includes a first part and a second part. The sample chamber, test strip slot, and flow channel are all formed on the first part. The sample chamber extends from a first end face of the first part to a second end face opposite to the first end face. The second part is sealed to the second end face of the first part. The flow channel and test strip slot are both open grooves formed on the second end face. The opening of the sample chamber on the second end face is recessed towards the first end face to form a connecting ring platform. The blood filtration membrane is connected to the connecting ring platform, and the flow channel is located on the side of the connecting ring platform closer to the second part.

[0009] In some embodiments, the sample cavity has an opening on the first end face that protrudes from the first end face to form a convex ring, and the upper cover has an annular connecting portion that is interference-fitted onto the convex ring.

[0010] In some embodiments, the pressing deformation portion and the annular connecting portion are integrally formed structures.

[0011] In some embodiments, each of the micropillars is formed on the end face of the second segment facing the first segment.

[0012] In some implementations, the first component and the second component are bonded and packaged as a single unit.

[0013] In some embodiments, the blood filtration membrane is welded to the connecting ring platform.

[0014] This invention provides a reagent kit for whole blood plasma separation. A blood filtration membrane is placed between the sample chamber and the test strip slot, and a pressing deformation part is provided on the top cover. When a whole blood sample is added to the sample chamber and reacts with the pre-placed reagents for extraction, the user (i.e., the testing operator) presses on the pressing deformation part, allowing the plasma in the whole blood sample to be filtered and separated through the blood filtration membrane and enter the test strip slot, where it comes into contact with the inserted test strip. This achieves plasma separation and subsequent testing. The reagent kit has a simple and compact structure, is easy and flexible to operate, avoids the use of complex devices and requires no additional equipment, significantly reducing manufacturing and purchase costs. It is particularly suitable for home use and facilitates the promotion of POCT technology at the grassroots level. The reagent kit of this application can be mass-produced with a simple process and can be used for the detection of whole blood plasma in biological and medical fields, especially in the field of immunodiagnostics. Attached Figure Description

[0015] Figure 1 This is a three-dimensional structural diagram (structural breakdown) of the reagent kit for whole blood plasma separation according to the present invention from a one-view perspective. Figure 2 yes Figure 1 A three-dimensional structural diagram of the first component of the main body of the reagent kit; Figure 3 yes Figure 1 A schematic diagram of the second component of the main body of the reagent kit; Figure 4 yes Figure 1 A schematic diagram of the structure of the upper cover.

[0016] The reference numerals in the attached figures are as follows: 1. Reagent kit body; 11. Sample chamber; 111. Connecting ring platform; 112. Convex ring; 12. Test strip slot; 13. Blood filtration membrane; 14. Flow channel; 15. Microcolumn; 2. Top cover; 21. Pressing deformation part; 22. Ring connecting part; 101. First part; 102. Second part. Detailed Implementation

[0017] See also Figures 1 to 4 As shown in the figure, according to an embodiment of the present invention, a whole blood plasma separation kit is provided, including a kit body 1 and a top cover 2. The kit body 1 has a sample chamber 11, a test strip slot 12, and a blood filtration membrane 13. The blood filtration membrane 13 is located between the sample chamber 11 and the test strip slot 12. The sample chamber 11 is pre-filled with a target reagent (the specific type can be reasonably selected according to the actual testing purpose). The test strip slot 12 is used to insert a test strip (not shown in the figure, the specific type can be reasonably selected according to the actual testing purpose). The top cover 2 is sealed and fastened to the opening of the sample chamber 11. The top cover 2 has a pressing deformation part 21. When force is applied to the pressing deformation part 21 in the direction towards the sample chamber 11, the plasma of the whole blood sample in the sample chamber 11 can enter the test strip slot 12 through the blood filtration membrane 13 to contact the test strip.

[0018] In this technical solution, a blood filtration membrane 13 is placed between the sample chamber 11 and the test strip slot 12, and a pressing deformation part 21 is provided on the upper cover 2. When a whole blood sample is added to the sample chamber 11 and reacts and extracts with the pre-placed reagents, the user (i.e., the test operator) presses and applies force to the pressing deformation part 21, so that the plasma in the whole blood sample can be filtered and separated through the blood filtration membrane 13 and enter the test strip slot 12, where it comes into contact with the test strip inserted therein, thereby realizing plasma separation and subsequent testing. The reagent kit has a simple and compact structure, is easy and flexible to operate, avoids the use of complex devices, and does not require corresponding equipment, significantly reducing manufacturing and purchase costs. It is especially suitable for home use and is conducive to the promotion of POCT technology at the grassroots level. The reagent kit in this application can be mass-produced and has a simple process, and can be used for the detection of whole blood plasma in the fields of biology and medicine, especially in the field of immunodiagnosis.

[0019] In some embodiments, the sample chamber 11 and the test strip slot 12 are connected by a flow channel 14. The specific flow area of ​​the flow channel 14 is reasonably selected according to the actual amount of whole blood sample. By setting the flow channel 14 to connect the sample 11 and the test strip slot 12, the flow rate of the filtered and separated plasma can be controlled, which is beneficial to improving the detection accuracy of the test strip.

[0020] In some embodiments, the flow channel 14 is provided with a plurality of micropillars 15 spaced apart. The diameter of the aforementioned micropillars 15 and the spacing between two adjacent micropillars 15 are reasonably selected according to actual needs. In this application, by providing a plurality of micropillars 15 in the flow channel 14, a secondary filtration can be formed on the filtered and separated plasma, which can further improve the detection accuracy of the test strip.

[0021] In one specific embodiment, the reagent kit body 1 includes a first part 101 and a second part 102. The sample chamber 11, the test strip slot 12, and the flow channel 14 are all formed on the first part 101. The sample chamber 11 extends from the first end face of the first part 101 to the second end face opposite to the first end face. The second part 102 is sealed and connected to the second end face of the first part 101. The flow channel 14 and the test strip slot 12 are both open grooves formed on the second end face. It is understood that the opening of the aforementioned open groove is sealed by the aforementioned second part 102. The opening of the sample chamber 11 on the second end face is recessed towards the first end face to form a connecting ring platform 111. The blood filtration membrane 13 is connected to the connecting ring platform 111, and the flow channel 14 is located on the side of the connecting ring platform 111 closer to the second part 102.

[0022] In this technical solution, the first part 101 and the second part 102 are interlocked to form a structure, so that the flow channel 14 and the test strip slot 12 on the first part 101 are both open groove structures, which can simplify the manufacturing process of these tiny flow channels.

[0023] In some embodiments, the first component 101 and the second component 102 are bonded and packaged as a single unit; the blood filtration membrane 13 is welded to the connecting ring 111, so that the connection between the components is reliable and stable.

[0024] In some embodiments, the sample cavity 11 has an opening on the first end face that protrudes from the first end face to form a convex ring 112, and the upper cover 2 has an annular connecting part 22, which is press-fitted onto the convex ring 112, thereby further simplifying the user's operation.

[0025] In some embodiments, the pressing deformation part 21 and the annular connecting part 22 are integrally molded structures. In one specific embodiment, the aforementioned upper cover 2 is formed by one-time injection molding of silicone.

[0026] In some embodiments, each of the micropillars 15 is formed on the end face of the second segment 102 facing the first segment 101.

[0027] It will be readily understood by those skilled in the art that the aforementioned advantageous methods can be freely combined and superimposed without conflict.

[0028] The above are merely preferred embodiments of this utility model and are not intended to limit the scope of this utility model. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of this utility model should be included within the protection scope of this utility model. The above are only preferred embodiments of this utility model. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the technical principles of this utility model, and these improvements and modifications should also be considered within the protection scope of this utility model.

Claims

1. A reagent kit for whole blood plasma separation, characterized in that, The kit includes a main body (1) and a top cover (2). The main body (1) has a sample chamber (11), a test strip slot (12), and a blood filtration membrane (13). The blood filtration membrane (13) is located between the sample chamber (11) and the test strip slot (12). The sample chamber (11) is pre-filled with a target reagent. The test strip slot (12) is used to insert a test strip for testing. The top cover (2) is sealed and fastened to the opening of the sample chamber (11). The top cover (2) has a pressing deformation part (21). When force is applied to the pressing deformation part (21) in a direction toward the sample chamber (11), the plasma of the whole blood sample in the sample chamber (11) can enter the test strip slot (12) through the blood filtration membrane (13) to contact the test strip for testing.

2. The reagent kit according to claim 1, characterized in that, The sample chamber (11) and the test strip slot (12) are connected by a flow channel (14).

3. The reagent kit according to claim 2, characterized in that, The flow channel (14) is provided with a plurality of micro-pillars (15) spaced apart.

4. The reagent kit according to claim 3, characterized in that, The main body (1) of the reagent kit includes a first part (101) and a second part (102). The sample chamber (11), the test strip slot (12) and the flow channel (14) are all formed on the first part (101). The sample chamber (11) extends from the first end face of the first part (101) to the second end face opposite to the first end face. The second part (102) is sealed and connected to the second end face of the first part (101). The flow channel (14) and the test strip slot (12) are both open slots formed on the second end face. The cavity of the sample chamber (11) on the second end face is recessed towards the first end face to form a connecting ring platform (111). The blood filtration membrane (13) is connected to the connecting ring platform (111). The flow channel (14) is located on the side of the connecting ring platform (111) close to the second part (102).

5. The reagent kit according to claim 4, characterized in that, The sample cavity (11) has an opening on the first end face that protrudes from the first end face to form a convex ring (112). The upper cover (2) has an annular connecting part (22), which is press-fitted onto the convex ring (112).

6. The reagent kit according to claim 5, characterized in that, The pressing deformation part (21) and the annular connecting part (22) are integrally formed structures.

7. The reagent kit according to claim 4, characterized in that, Each of the micropillars (15) is formed on the end face of the second part (102) facing the first part (101).

8. The reagent kit according to claim 4, characterized in that, The first component (101) and the second component (102) are bonded and packaged together as one unit.

9. The reagent kit according to claim 4, characterized in that, The blood filtration membrane (13) is welded to the connecting ring platform (111).

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