A test device for separating rhizosphere nitrogen-fixing microorganisms in situ

Abstract

The utility model discloses a kind of test devices of separating type in-situ marking rhizosphere nitrogen-fixing microorganism, comprising: planting pot, planting pot is hollow cuboid structure, and planting pot side surface is equipped with through-hole;Partition, partition is provided with two groups, and two groups of partition are cross detachably connected in planting pot, and the top center position of partition is equipped with semicircular groove, and semicircular groove is used to plant plants;Annular gas injection channel, annular gas injection channel is fixed in planting pot inner bottom position, and a plurality of gas injection holes are formed in annular gas injection channel;Gas supply assembly, gas supply assembly output end is connected with pipeline, and pipeline one end passes through through-hole and is connected with annular gas injection channel;Wherein, gas flowmeter is installed on pipeline.The utility model effectively solves the technical problems that traditional method cannot cover deep soil and marking efficiency is not high, and provides guarantee for isotope tracing marking rhizosphere nitrogen-fixing microorganism.

Description

Technical Field

[0001] This invention relates to the field of plant rhizosphere microbial analysis technology, and in particular to a test device for septate in-situ labeling of rhizosphere nitrogen-fixing microorganisms. Background Technology

[0002] The rhizosphere generally refers to the area within a few millimeters of the plant root surface; it is the soil micro-region directly affected by plant root activity. In the plant-soil system, rhizosphere microorganisms are the most active components of the soil microbial ecosystem. Research on nitrogen-fixing microorganisms mainly involves isolating and culturing nitrogen-fixing strains indoors, or using techniques such as sequencing clone libraries of the nitrogen-fixing functional gene nifH, denaturing gradient gel electrophoresis, and analysis of terminal restriction enzyme polymorphisms to study the population diversity of rhizosphere nitrogen-fixing microorganisms; however, the analytical throughput is very low. With the rapid development of high-throughput sequencing technology, the method of studying the diversity of nitrogen-fixing microbial communities through nifH amplicon high-throughput sequencing has become quite mature.

[0003] In recent years, airtight plant growth control boxes have been used... 15 This study investigated nitrogen-fixing microorganisms in the rice-soil system using N2 gas feeding combined with nifH amplicon high-throughput sequencing. The results showed that rice cultivation significantly altered the structure of the rhizosphere soil nitrogen-fixing microbial community. This method extends the research scale from indoor potted plants to in-situ field applications, but it still has technical limitations. During plant growth, the airtight plant growth control chamber... 15 N2 gas can come into direct contact with the surface soil of rice pots or the surface of flooded water, but it cannot fully penetrate into the deep soil region of the rice-soil system. This affects the efficiency of isotope labeling of rhizosphere nitrogen-fixing microorganisms, thus making it impossible to accurately identify rhizosphere nitrogen-fixing microorganisms in this deep soil region and seriously underestimating the diversity of rhizosphere nitrogen-fixing microbial communities in the rice-soil system.

[0004] Based on the above-mentioned technical problems, this utility model provides a test device for isolated in-situ labeling of rhizosphere nitrogen-fixing microorganisms. Utility Model Content

[0005] The purpose of this invention is to provide a separate in-situ labeled rhizosphere nitrogen-fixing microorganism experimental device to solve the problems existing in the prior art.

[0006] To achieve the above objectives, this utility model provides the following solution: This utility model provides a test device for isolated in-situ labeled rhizosphere nitrogen-fixing microorganisms, comprising: A planting pot, wherein the planting pot has a hollow rectangular structure and a through hole is provided on the side of the planting pot; The partition has two sets, which are detachably connected to the planting pot in a cross shape. A semi-circular groove is provided at the top center of the partition for planting plants. An annular air injection channel is fixed at the bottom of the planting pot, and several air injection holes are provided on the annular air injection channel. A gas supply assembly, the output end of which is connected to a pipeline, one end of which passes through the through hole and is connected to the annular gas injection channel; A gas flow meter is installed on the pipeline.

[0007] According to the experimental device for the partitioned in-situ labeled rhizosphere nitrogen-fixing microorganisms provided by this utility model, the gas supply component includes a gas storage device, and the output port of the gas storage device is connected to one end of the pipeline.

[0008] According to the experimental device for the partitioned in-situ labeled rhizosphere nitrogen-fixing microorganisms provided by this utility model, the inner contour of the planting pot has a length of 20cm, a width of 20cm, and a height of 30cm.

[0009] According to the experimental device for the partitioned in-situ labeled rhizosphere nitrogen-fixing microorganisms provided by this utility model, the pipeline is a TPU polyurethane hose with an inner diameter of 4 mm and an outer diameter of 6 mm.

[0010] According to the experimental device for the in-situ labeled rhizosphere nitrogen-fixing microorganisms provided by this utility model, a connecting pipe is installed on the annular gas injection channel, and the connecting pipe is connected to the pipeline by a quick-release connector.

[0011] According to the experimental device for the isolated in-situ labeled rhizosphere nitrogen-fixing microorganisms provided by this utility model, the gas storage device is a steel cylinder with a gas pressure reducing valve or a gas bag with a micro gas supply pump.

[0012] The present invention discloses the following technical effects: This device, through its bottom annular gas injection channel and multiple gas injection holes, overcomes the limitations of traditional airtight boxes that can only act on the surface soil. It allows 15N2 labeled gas to diffuse evenly upwards from the bottom, completely penetrating into the deep soil region. This significantly improves the isotope labeling efficiency for nitrogen-fixing microorganisms in the rhizosphere of deep soil, effectively solving the technical problems of traditional methods being unable to cover deep soil and resulting in incomplete labeling. It provides a guarantee for the comprehensive capture of isotope-traceable nitrogen-fixing microorganisms in the rhizosphere.

[0013] The device features a detachable cross-shaped partition design, allowing for adjustments to the partition installation method or removal of the partitions according to research needs, adapting to different plant cultivation and experimental designs. The transparent planting pots facilitate observation of the experimental process, and the convenient connection between the pipeline and the gas injection channel simplifies the device assembly process. Meanwhile, the application of a gas flow meter enables precise control of gas supply, making it suitable not only for indoor potted plant experiments but also for flexible expansion to various research scenarios such as in-situ field experiments, thus enhancing the device's practicality and promotional value. Attached Figure Description

[0014] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0015] Figure 1 This is a schematic diagram of the overall structure of the experimental device for the partitioned in-situ labeled rhizosphere nitrogen-fixing microorganisms of this utility model; Figure 2 This is a schematic diagram of the internal structure of the planting pot of this utility model.

[0016] Figure 3 This is a schematic diagram of the structure of the planting pot of this utility model.

[0017] The components include: 1. Planting pot; 2. Partition; 3. Semi-circular groove; 4. Annular air injection channel; 5. Gas flow meter; 6. Gas storage device; and 7. Pipeline. Detailed Implementation

[0018] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0019] To make the above-mentioned objectives, features and advantages of this utility model more apparent and understandable, the utility model will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0020] Reference Figures 1-3 This invention provides a test device for isolated in-situ labeled rhizosphere nitrogen-fixing microorganisms, comprising: Planting pot 1, which is a hollow rectangular structure, has through holes on its side. The partition 2 is provided in two sets. The two sets of partition 2 are detachably connected in a cross shape inside the planting pot 1. A semi-circular groove 3 is opened at the center of the top of the partition 2. The semi-circular groove 3 is used for planting plants. An annular air injection channel 4 is fixed at the bottom of the planting pot 1, and several air injection holes are provided on the annular air injection channel 4. The gas supply component has a pipe 7 connected to its output end. One end of the pipe 7 passes through a through hole and is connected to the annular gas injection channel 4. Among them, a gas flow meter 5 is installed on pipeline 7.

[0021] Before the experiment began, two sets of removable, cross-shaped partitions 2 were installed inside the hollow rectangular planting pot 1. The target plant (such as rice) was planted in the semi-circular groove 3 at the center of the top of the partition 2, and the corresponding soil was filled into the planting pot 1, allowing the plant roots to grow naturally in the soil. The partitions 2 divided the soil into different areas, ensuring the independence of the rhizosphere microenvironment. Then, the annular aeration channel 4 was fixed to the bottom of the planting pot 1. Through the through-hole on the side of the planting pot 1, the pipe 7 connected to the air supply component was connected to the annular aeration channel 4, ensuring a good seal on the pipe 7. At the same time, the gas flow meter 5 installed on the pipe 7 was checked to ensure it was working properly. After the experiment started, the air supply component delivered a gas containing... 15 The labeled N2 gas is evenly released through several injection holes in the annular injection channel 4. Utilizing the natural diffusion properties of gas, it permeates upwards from the bottom of the planting pot 1 into all soil layers, including deeper soil areas. During gas delivery, the gas flow rate and supply are precisely controlled by the gas flow meter 5 to ensure a stable concentration of the labeled gas in the soil, allowing rhizosphere nitrogen-fixing microorganisms influenced by plant root activity to interact with the surrounding environment. 15 The N2 labeling gas fully contacts and reacts, achieving in-situ labeling of rhizosphere nitrogen-fixing microorganisms. The separation effect of partition 2 avoids cross-interference between microorganisms in different areas, ensuring the targetedness and effectiveness of the labeling process.

[0022] In a further optimized version, the gas supply component includes a gas storage device 6, the output port of which is connected to one end of the pipeline 7.

[0023] The gas supply assembly employs a combined design of a steel cylinder or gas bag with a gas pressure reducing valve and a gas bag with a miniature gas supply pump, achieving stable storage and precise delivery of the marked gas. The steel cylinder or gas bag with the gas pressure reducing valve serves as the gas storage unit 6, safely storing high-purity gas. 15 The N2-labeled gas has its initial pressure precisely regulated by a pressure reducing valve to prevent damage to the pipeline or loss of gas flow rate due to excessive pressure. At the same time, the flexible structure of the gas bag can adapt to pressure changes during the gas output process, ensuring the continuity of gas supply.

[0024] The design was further optimized so that the inner contour of planting pot 1 is 20cm long, 20cm wide, and 30cm high.

[0025] The design was further optimized so that pipe 7 is a TPU polyurethane hose with an inner diameter of 4mm and an outer diameter of 6mm.

[0026] The scheme was further optimized by installing a connecting pipe on the annular gas injection channel 4, and the connecting pipe was connected to the pipeline 7 via a quick-release connector.

[0027] In a further optimized design, the gas storage device 6 is a steel cylinder with a gas pressure reducing valve or a gas bag with a miniature gas supply pump.

[0028] In this embodiment, multiple annular air injection holes can be provided on the outer wall of the planting pot 1, so that the device can be applied to different soil depths. In this way, the entire soil can be completely labeled under potted / in-situ soil column simulation experiments at different soil depths, thereby improving the efficiency of isotope labeling.

[0029] In the description of this utility model, it should be understood that the terms "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this utility model and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

[0030] The embodiments described above are merely preferred embodiments of the present utility model and are not intended to limit the scope of the present utility model. Various modifications and improvements made to the technical solutions of the present utility model by those skilled in the art without departing from the spirit of the present utility model should fall within the protection scope defined by the claims of the present utility model.

Claims

1. A test apparatus for isolated in-situ labeling of rhizosphere nitrogen-fixing microorganisms, characterized in that, include: Planting pot (1), the planting pot (1) is a hollow cuboid structure, and the side of the planting pot (1) is provided with through holes; The partition (2) is provided in two sets. The two sets of partitions (2) are detachably connected to the planting pot (1) in a cross shape. A semi-circular groove (3) is provided at the center of the top of the partition (2). The semi-circular groove (3) is used for planting plants. An annular air injection channel (4) is fixed at the bottom of the planting pot (1), and several air injection holes are provided on the annular air injection channel (4). An air supply assembly is provided, with a pipeline (7) connected to its output end. One end of the pipeline (7) passes through the through hole and is connected to the annular air injection channel (4). A gas flow meter (5) is installed on the pipeline (7).

2. The experimental device for isolated in-situ labeled rhizosphere nitrogen-fixing microorganisms according to claim 1, characterized in that, The gas supply assembly includes a gas storage device (6), the output port of which is connected to one end of the pipeline (7).

3. The experimental device for isolated in-situ labeled rhizosphere nitrogen-fixing microorganisms according to claim 1, characterized in that, The inner contour of the planting pot (1) is 20cm long, 20cm wide, and 30cm high.

4. The experimental device for isolated in-situ labeled rhizosphere nitrogen-fixing microorganisms according to claim 1, characterized in that, The pipeline (7) is a TPU polyurethane hose with an inner diameter of 4 mm and an outer diameter of 6 mm.

5. The experimental device for isolated in-situ labeled rhizosphere nitrogen-fixing microorganisms according to claim 1, characterized in that, A connecting pipe is installed on the annular gas injection channel (4), and the connecting pipe is connected to the pipeline (7) through a quick-release connector.

6. The experimental device for isolated in-situ labeled rhizosphere nitrogen-fixing microorganisms according to claim 2, characterized in that, The gas storage device (6) is a steel cylinder with a gas pressure reducing valve or a gas bag with a miniature gas supply pump.

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