A soybean cyst nematode hatching device

Abstract

The utility model belongs to cyst nematode hatching technical field, specifically is a kind of soybean cyst nematode hatching device, including thermostat, the side wall of thermostat is equipped with cabinet door, the bottom of cabinet door is equipped with temperature control switch, the top of thermostat is equipped with two circulating fans, the middle part of temperature control switch is equipped with temperature display, hatching tray is installed in the thermostat, the middle part of hatching tray is equipped with several mesh screens with 200 microns aperture, the bottom of hatching tray is fixedly connected with collection tray, collection assembly is installed in the bottom of collection tray, liquid injection assembly is fixedly connected in the middle part of thermostat, the collection assembly includes clamping strip, the bottom of collection tray is fixedly connected with clamping strip one end, the bottom of collection tray is equipped with inclined groove, through the above structure, larvae can flow into collection box interior with liquid quickly, reduce the physical damage of larvae to be subjected to any external force shear or adsorption generation, can keep the activity and integrity of larvae, effectively reduce larvae loss and operation time, improve larvae collection efficiency.

Description

Technical Field

[0001] This invention belongs to the field of soybean cyst nematode hatching technology, specifically a soybean cyst nematode hatching device. Background Technology

[0002] Soybean cyst nematode is a highly specialized plant parasitic nematode, and the soybean cyst nematode disease it causes is one of the most destructive soybean diseases.

[0003] Soybean cyst nematode hatching refers to the process by which the embryos of the eggs mature under suitable conditions, and the second-stage larvae break through the eggshell and are released. Before hatching, most of the eggs in the cysts enter a physiological dormancy period, which requires root secretions from host plants such as soybeans to awaken them. After receiving the signal, the embryos in the eggs begin metabolic activities, complete the final developmental stage, and form second-stage larvae with infectivity. The larvae emerge from the eggshell and begin to search for and infect soybean roots. Traditionally, larvae collection requires the use of a pipette to repeatedly and carefully aspirate liquid in a shallow dish. The suction force can easily cause physical damage to the larvae, and some larvae attached to the side walls are easily missed. In addition, the operation is relatively time-consuming.

[0004] Therefore, this utility model provides a soybean cyst nematode hatching device, which is beneficial for more efficient collection of soybean cyst nematodes. Utility Model Content

[0005] The purpose of this invention is to address the problems of difficulty in collecting hatched larvae, the risk of physical damage to the larvae, or omissions during the collection process by proposing a soybean cyst nematode hatching device to solve the problems mentioned in the background art.

[0006] The technical solution adopted by this utility model to solve its technical problem is as follows: A soybean cyst nematode hatching device of this utility model includes a constant temperature chamber; a door is installed on the side wall of the constant temperature chamber; a temperature control switch is provided at the bottom of the door; two circulating fans are installed on the top of the constant temperature chamber; a temperature display is provided in the center of the temperature control switch; an incubation tray is installed on the inner side wall of the constant temperature chamber; several sieves with a aperture of 200 micrometers are opened in the center of the incubation tray; a collection tray is fixedly connected to the bottom of the incubation tray; a collection component is installed at the bottom of the collection tray; and a collection assembly is fixedly connected to the center of the constant temperature chamber. The liquid injection assembly includes a locking strip, which is fixed to one end of the bottom of the collection tray. A sloping groove is formed at the bottom of the collection tray, corresponding to the locking strip. A sealing plate is installed in the middle of the locking strip. Two fixing frames are fixed to the bottom of the collection tray, and a collection box is installed in the middle of the two fixing frames. This structure allows larvae to flow quickly into the collection box with the liquid, reducing physical damage caused by shearing or adsorption from any external force, maintaining the activity and integrity of the larvae, effectively reducing larval loss and operation time, and improving larval collection efficiency.

[0007] Preferably, the liquid injection assembly includes a liquid injection tube; the liquid injection tube is fixed to the inner side wall of the constant temperature chamber; the liquid injection tube penetrates the top of the constant temperature chamber; an elastic rod is fixed to the top of the constant temperature chamber; a rubber stopper is fixed to the end of the elastic rod; the rubber stopper is installed on the top of the liquid injection tube; the above structure can reduce the sudden drop in temperature and loss of humidity inside the constant temperature chamber caused by opening the chamber door to add liquid, providing a continuous and consistent optimal environment for the hatching of cyst nematodes, and can effectively reduce the introduction of pollutants from the outside air into the hatching solution when the chamber door is opened, thereby reducing microbial contamination.

[0008] Preferably, the inner sidewall of the constant temperature chamber has two mounting grooves; the two mounting grooves are arranged opposite each other; a positioning plate is slidably connected to the middle of the mounting groove; a rubber pad is fixed to the outer sidewall of the positioning plate; the above structure can quickly fix the incubation tray in the designated position, and the friction of the rubber pad keeps the incubation tray stable inside the constant temperature chamber, maintaining the integrity of the sample and the accuracy of the data.

[0009] Preferably, a phase change energy storage plate is fixedly connected to the middle of the constant temperature chamber; the phase change energy storage plate is fixedly connected to the outer wall of the constant temperature chamber; the above structure can actively suppress temperature fluctuations inside the constant temperature chamber and provide a stable incubation environment.

[0010] Preferably, a sealing groove is provided on the inner side wall of the door; a sealing ring is fixedly connected to the side wall of the constant temperature chamber; the sealing groove and the sealing ring are correspondingly arranged; the above structure can effectively fill the tiny gaps between the constant temperature chamber and the door, reduce the heat loss inside the constant temperature chamber, and form a good seal.

[0011] Preferably, the inner wall of the hatching tray is sloped; this structure facilitates observation of the cyst nematode hatching process and improves monitoring convenience.

[0012] The beneficial effects of this utility model are as follows:

[0013] 1. The soybean cyst nematode hatching device of this utility model, through the above structure, enables larvae to flow quickly into the collection box with the liquid, reducing physical damage to the larvae caused by any external shearing or adsorption, maintaining the activity and integrity of the larvae, effectively reducing larval loss and operation time, and improving larval collection efficiency.

[0014] 2. The soybean cyst nematode hatching device of this utility model, through the above structure, can reduce the sudden drop in temperature and loss of humidity inside the constant temperature chamber caused by opening the chamber door to add liquid, providing a continuous and consistent optimal environment for cyst nematode hatching, and can effectively reduce the introduction of pollutants from the outside air into the hatching solution when the chamber door is opened, thereby reducing microbial contamination. Attached Figure Description

[0015] The present invention will be further described below with reference to the accompanying drawings.

[0016] Figure 1 This is a perspective view of the present invention;

[0017] Figure 2 This is a schematic diagram of the sealing ring structure in this utility model;

[0018] Figure 3 This is a schematic diagram of the structure of the constant temperature chamber in this utility model;

[0019] Figure 4 This is an exploded view of the collecting components in this utility model;

[0020] Figure 5 This is a schematic diagram of the liquid injection tube in this utility model.

[0021] In the diagram: 1. Incubator; 10. Temperature display; 11. Door; 12. Temperature control switch; 13. Circulating fan; 14. Incubation tray; 15. Screen; 16. Collection tray; 2. Collection assembly; 21. Locking strip; 22. Inclined groove; 23. Sealing plate; 24. Fixing frame; 25. Collection box; 3. Liquid injection assembly; 31. Liquid injection tube; 32. Elastic rod; 33. Rubber stopper; 4. Installation slide; 41. Positioning plate; 42. Rubber pad; 5. Phase change energy storage plate; 6. Sealing groove; 61. Sealing ring. Detailed Implementation

[0022] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of the present utility model.

[0023] Specific implementation examples are given below.

[0024] like Figures 1 to 5As shown in the figure, a soybean cyst nematode hatching device according to an embodiment of the present invention includes a constant temperature chamber 1; a door 11 is installed on the side wall of the constant temperature chamber 1; a temperature control switch 12 is provided at the bottom of the door 11; two circulating fans 13 are installed on the top of the constant temperature chamber 1; a temperature display 10 is provided in the middle of the temperature control switch 12; an incubation tray 14 is installed on the inner side wall of the constant temperature chamber 1; a plurality of sieves 15 with a aperture of 200 micrometers are opened in the middle of the incubation tray 14; a collection tray 16 is fixedly connected to the bottom of the incubation tray 14; the collection tray... A collection component 2 is installed at the bottom of the collection tray 16; a liquid injection component 3 is fixedly connected to the middle of the constant temperature chamber 1; the collection component 2 includes a retaining strip 21; the retaining strip 21 is fixedly connected to one end of the bottom of the collection tray 16; a sloping groove 22 is opened at the bottom of the collection tray 16; the sloping groove 22 is correspondingly set at the position of the retaining strip 21; a sealing plate 23 is installed in the middle of the retaining strip 21; two fixing brackets 24 are fixedly connected to the bottom of the collection tray 16; a collection box 25 is installed in the middle of the two fixing brackets 24; during operation, the constant temperature chamber 1 is opened and the target temperature is set, and liquid is injected into the collection tray 16. Add an appropriate amount of incubation solution to the incubation tray 14, ensuring the solution level just touches the bottom. Place the sporangia on the incubation tray 14 and place it in the preheated incubator 1. Close the door 11 and periodically check the temperature control display of the incubator 1 to maintain a stable temperature. During incubation, the larvae pass through the screen 15 into the liquid inside the collection tray 16. At the set time, open the door 11 and remove the incubation tray 14. Then, pull the sealing plate 23 out from the middle of the retaining strip 21. The collection tray 16 tilts to one side, and the larvae and liquid slide down the inclined groove 22 into the collection box 25 for rapid collection. Pull the collection box 25 out from the middle of the fixing frame 24 to transfer all the larvae to the counting plate. Count and record the larvae under a microscope. This structure allows the larvae to flow quickly into the collection box 25 with the liquid, reducing physical damage caused by any external shearing or adsorption, maintaining the activity and integrity of the larvae, effectively reducing larvae loss and operation time, and improving larvae collection efficiency.

[0025] like Figure 3 and Figure 5As shown, the liquid injection assembly 3 includes a liquid injection tube 31; the liquid injection tube 31 is fixed to the inner wall of the constant temperature chamber 1; the liquid injection tube 31 passes through the top of the constant temperature chamber 1; an elastic rod 32 is fixed to the top of the constant temperature chamber 1; a rubber stopper 33 is fixed to the end of the elastic rod 32; the rubber stopper 33 is installed on the top of the liquid injection tube 31; during operation, when it is necessary to replenish the incubation solution during the incubation process, simply pull the rubber stopper 33 out from the end of the liquid injection tube 31, inject the fresh incubation solution into the end of the liquid injection tube 31, and let it flow through the liquid injection tube 31 by gravity, and the liquid flows directly out from the other end, accurately and steadily injecting into the designated collection tray 16. After replenishment, the rubber stopper 33 is plugged and sealed at the end of the liquid injection tube 31. The above structure can reduce the sudden drop in temperature and loss of humidity inside the constant temperature chamber 1 caused by opening the chamber door 11 to replenish the solution, providing a continuous and consistent optimal environment for the incubation of cyst nematodes, and can effectively reduce the introduction of pollutants from the outside air into the incubation solution when the chamber door 11 is opened, thus reducing microbial contamination.

[0026] like Figure 3 and Figure 4 As shown, two mounting grooves 4 are provided on the inner side wall of the constant temperature chamber 1; the two mounting grooves 4 are arranged opposite each other; a positioning plate 41 is slidably connected to the middle of the mounting groove 4; a rubber pad 42 is fixed to the outer side wall of the positioning plate 41; during operation, when the incubation tray 14 is placed inside the constant temperature chamber 1, the positioning plate 41 is first placed in the position corresponding to the mounting groove 4, and the incubation tray 14 is moved inward. At this time, the rubber pad 42 is tightly fitted with the inner side wall of the mounting groove 4, and the incubation tray 14 is fixed inside the constant temperature chamber 1 by the positioning plate 41. After the incubation tray 14 is in place, the rubber pad 42 on the positioning plate 41 and the inner wall of the mounting groove 4 form an interference fit, filling the gap between the positioning plate 41 and the mounting groove 4. Through the above structure, the incubation tray 14 can be quickly fixed in the designated position. The friction of the rubber pad 42 keeps the incubation tray 14 stable inside the constant temperature chamber 1, maintaining the integrity of the sample and the accuracy of the data.

[0027] like Figure 1 As shown, a phase change energy storage plate 5 is fixedly connected to the middle of the constant temperature chamber 1; the phase change energy storage plate 5 is fixedly connected to the outer wall of the constant temperature chamber 1; during operation, when the internal temperature of the constant temperature chamber 1 is higher than the set point during the incubation process, the phase change energy storage plate 5 will absorb excess heat from the outer wall of the constant temperature chamber 1; when the internal temperature of the constant temperature chamber 1 is lower than the set point, the internal temperature of the constant temperature chamber 1 begins to drop; when the temperature drops to the freezing point of the phase change energy storage plate 5, the stored latent heat is released. Through the above structure, the temperature fluctuation inside the constant temperature chamber 1 can be actively suppressed, providing a stable incubation environment.

[0028] like Figure 2As shown, a sealing groove 6 is provided on the inner side wall of the door 11; a sealing ring 61 is fixedly connected to the side wall of the constant temperature chamber 1; the sealing groove 6 and the sealing ring 61 are correspondingly arranged; during operation, when the door 11 is closed, the sealing ring 61 enters the corresponding sealing groove 6, and the door 11 and the constant temperature chamber 1 are sealed by the sealing ring 61. The above structure can effectively fill the tiny gaps between the constant temperature chamber 1 and the door 11, reduce the heat loss inside the constant temperature chamber 1, and form a good seal.

[0029] like Figure 4 As shown, the inner wall of the hatching tray 14 is set with an incline. When the cyst nematodes are placed in the middle of the hatching tray 14 during operation, they will naturally concentrate in the central area of ​​the hatching tray 14. The above structure makes it easier to observe the hatching process of the cyst nematodes and improves the convenience of monitoring.

[0030] During operation, turn on the incubator 1 and set the target temperature. Add an appropriate amount of incubation solution to the collection tray 16, ensuring the solution level is just touching the bottom of the incubation tray 14. Place the sporangia on the incubation tray 14 and place it in the center of the preheated incubator 1. Close the door 11 and periodically check the temperature control display of the incubator 1 to maintain a stable temperature. During incubation, the larvae will pass through the screen 15 and enter the liquid inside the collection tray 16. At the set time, open the door 11, remove the incubation tray 14, and then pull the sealing plate 23 out from the middle of the retaining strip 21. The collection tray 16 is tilted to one side, allowing the larvae and liquid to slide down the inclined groove 22 into the collection box 25 for rapid collection. The collection box 25 can then be pulled out from the middle of the fixing frame 24 to transfer all the larvae to a counting plate. The larvae are counted and recorded under a microscope. During hatching, when additional hatching solution is needed, simply pull the rubber stopper 33 from the end of the injection tube 31, inject fresh hatching solution into the end of the injection tube 31, and allow it to flow through the injection tube 31 by gravity, with the liquid flowing directly out from the other end, precisely and steadily injected into the designated collection tray 16. After replenishment, seal the end of the injection tube 31 by plugging the rubber stopper 33. When placing the incubation tray 14 inside the incubator 1, first place the positioning plate 41 corresponding to the mounting groove 4, and then move the incubation tray 14 inward. At this time, the rubber pad 42 is tightly fitted with the inner wall of the mounting groove 4. The positioning plate 41 fixes the incubation tray 14 inside the incubator 1. After the incubation tray 14 is in place, the rubber pad 42 on the positioning plate 41 and the inner wall of the mounting groove 4 form an interference fit, filling the gap between the positioning plate 41 and the mounting groove 4. During the incubation process... During the process, when the internal temperature of the constant temperature chamber 1 is higher than the set point, the phase change energy storage plate 5 will absorb excess heat from the outer wall of the constant temperature chamber 1. When the internal temperature of the constant temperature chamber 1 is lower than the set point, the internal temperature of the constant temperature chamber 1 begins to drop. When the temperature drops to the freezing point of the phase change energy storage plate 5, the stored latent heat is released. When the chamber door 11 is closed, the sealing ring 61 enters the corresponding sealing groove 6 and seals the chamber door 11 and the constant temperature chamber 1 through the sealing ring 61. When the cyst nematodes are placed in the middle of the hatching tray 14, they will naturally concentrate in the central area of ​​the hatching tray 14.

[0031] The foregoing has shown and described the basic principles, main features, and advantages of this utility model. Those skilled in the art should understand that this utility model is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of this utility model. Various changes and modifications can be made to this utility model without departing from its spirit and scope, and all such changes and modifications fall within the scope of the claimed utility model. The scope of protection of this utility model is defined by the appended claims and their equivalents.

Claims

1. A soybean cyst nematode hatching device, comprising a constant temperature chamber (1); characterized in that: The constant temperature chamber (1) has a door (11) installed on its side wall; a temperature control switch (12) is provided at the bottom of the door (11); two circulating fans (13) are installed on the top of the constant temperature chamber (1); a temperature display (10) is provided in the middle of the temperature control switch (12); an incubation tray (14) is installed on the inner side wall of the constant temperature chamber (1); several sieves (15) with a aperture of 200 micrometers are opened in the middle of the incubation tray (14); a collection tray (16) is fixed to the bottom of the incubation tray (14); a collection component (2) is installed at the bottom of the collection tray (16); and a liquid injection component (3) is fixed to the middle of the constant temperature chamber (1).

2. The soybean cyst nematode hatching device according to claim 1, characterized in that: The collection component (2) includes a retaining strip (21); the retaining strip (21) is fixed to one end of the bottom of the collection tray (16); the bottom of the collection tray (16) is provided with a slanted groove (22); the slanted groove (22) is correspondingly set at the position of the retaining strip (21); a sealing plate (23) is installed in the middle of the retaining strip (21); two fixing brackets (24) are fixed to the bottom of the collection tray (16); a collection box (25) is installed in the middle of the two fixing brackets (24).

3. The soybean cyst nematode hatching device according to claim 1, characterized in that: The liquid injection assembly (3) includes a liquid injection tube (31); the liquid injection tube (31) is fixed to the inner wall of the constant temperature chamber (1); the liquid injection tube (31) passes through the top of the constant temperature chamber (1); an elastic rod (32) is fixed to the top of the constant temperature chamber (1); a rubber stopper (33) is fixed to the end of the elastic rod (32); the rubber stopper (33) is installed on the top of the liquid injection tube (31).

4. The soybean cyst nematode hatching device according to claim 1, characterized in that: The constant temperature chamber (1) has two mounting grooves (4) on its inner side wall; the two mounting grooves (4) are arranged opposite to each other; a positioning plate (41) is slidably connected in the middle of the mounting groove (4); a rubber pad (42) is fixed to the outer side wall of the positioning plate (41).

5. The soybean cyst nematode hatching device according to claim 1, characterized in that: A phase change energy storage plate (5) is fixedly connected to the middle of the constant temperature chamber (1); the phase change energy storage plate (5) is fixedly connected to the outer wall of the constant temperature chamber (1).

6. The soybean cyst nematode hatching device according to claim 1, characterized in that: The inner side wall of the door (11) is provided with a sealing groove (6); the side wall of the constant temperature box (1) is fixed with a sealing ring (61); the sealing groove (6) and the sealing ring (61) are respectively provided.

7. The soybean cyst nematode hatching device according to claim 1, characterized in that: The inner wall of the incubation tray (14) is sloped.

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