A whole blood separation device and a plasma separation device

Abstract

The utility model provides a kind of whole blood separation device, the device is matched with syringe to constitute plasma separation device use, after syringe completes the collection of blood, needle is detached, and adapter whole blood separation device is directly utilized the piston assembly of syringe to carry out pressurization, whole blood separation device carries out separation to whole blood and obtains plasma sample, whole process is fast, simple and convenient. Whole blood separation device includes first cylinder, second cylinder, blood filter membrane and containing cavity, first cylinder and second cylinder are connected along the same axial arrangement, blood filter membrane is placed on the port connecting surface of two cylinder connection, and the second port on first cylinder is used to cover and compact blood filter membrane in the fourth port for plasma outflow, avoid whole blood leakage, and plasma filtration effect is good.

Description

Technical Field

[0001] This utility model belongs to the field of biological detection technology, specifically relating to a whole blood separation device and a plasma separation device. Background Technology

[0002] Blood samples include whole blood and plasma. Whole blood samples are usually collected by venous blood collection, which involves using a syringe to puncture a vein to obtain whole blood samples. Plasma samples can be obtained from whole blood samples by filtration separation methods. This method utilizes the physical size of hemoglobin and plasma to design corresponding filter membranes, microcolumns, or microspheres. Hemoglobin in whole blood is captured or retained by the filter membrane, microcolumn, or microspheres, while plasma passes through the pores and is collected to obtain plasma samples. For example, the Chinese patent application CN202420524812.5, entitled "Plasma Separator," uses the aforementioned filtration separation method to separate whole blood samples into plasma samples. This plasma separator includes an upper shell, a lower shell, a connecting belt, a pressing mechanism, and a filter membrane. The upper shell comprises an upper half-shell and a lower half-shell with sequentially increasing outer diameters, connected to each other. The bottom of the lower half-shell has a bottom shell with a through hole. The lower shell includes a lower connecting shell and a lower shell plate. The upper shell is connected to the connecting ring of the lower shell via a slot. An installation cavity is formed between the bottom shell and the lower connecting shell. The filter membrane is placed inside the installation cavity. A raised cylindrical liquid collection part is provided on the lower shell plate. The usage method is as follows: After transferring the collected whole blood sample to the plasma separator through the upper shell, the pressing mechanism is pressed into the upper shell to form a sealed space. Then, the pressing mechanism is pressed down, and the plasma in the whole blood sample passes through the filter membrane in the installation cavity and enters the liquid collection part on the lower connecting shell, completing the plasma separation process. Although the separator accelerates the filtration of whole blood by using air pressure, the entire separator structure is relatively complex. It requires the upper shell and the pressing mechanism to work together to complete the air pressurization process. In addition, the blood sample needs to be transferred from the syringe to the separator before pressurization. The whole process includes steps such as transfer, pressing into the seal and pressing down, which makes the operation quite cumbersome. Utility Model Content

[0003] In view of this, the purpose of this utility model is to provide a whole blood separation device with a simple structure. It is used in conjunction with a syringe barrel to form a plasma separation device. After the syringe completes the blood collection, the syringe is used to apply pressure, and the whole blood separation device separates the whole blood to obtain a plasma sample. The whole process is fast and convenient.

[0004] The whole blood separation device provided by this utility model includes a first cylinder, a second cylinder, a blood filtration membrane, and a receiving cavity, wherein... The first cylindrical body includes a first port, a second port, and a first cavity, wherein the first port and the second port are disposed opposite to each other and are respectively connected to the first cavity; The second cylinder includes a third port, a fourth port, and a second cavity, wherein the third port and the fourth port are arranged opposite to each other and are respectively connected to the second cavity; The third port is axially sleeved on the outer periphery of the second port and is snapped and sealed with the second port. The blood filtration membrane is placed at the bottom of the second cavity and covers the fourth port. The second port and the blood filtration membrane are correspondingly abutted against each other. The fourth port is connected to the receiving cavity.

[0005] The whole blood separation device has a simple structure. The first cylinder and the second cylinder are connected along the same axis. The blood filtration membrane is placed on the connecting surface of the port where the two cylinders are connected. The second port on the first cylinder covers and presses the blood filtration membrane onto the fourth port where the plasma flows out, thus preventing whole blood leakage and achieving good plasma filtration effect.

[0006] Furthermore, the second port has rounded corners around its perimeter to facilitate a smooth connection between the second port and the third port.

[0007] Furthermore, the hollow volume of the receiving cavity is 8-20 μl to facilitate quantitative separation of plasma.

[0008] Furthermore, the hollow width h of the receiving cavity is 0.3-0.5mm to avoid the hollow width of the receiving cavity being too large, which would affect the capillary force of the receiving cavity, and too small, which would increase the processing difficulty of the device.

[0009] Furthermore, the receiving cavity is a hollow cylinder, and the fourth port communicates with the receiving cavity through the side circumference of the cylinder. The receiving cavity is selected in a hollow cylindrical shape to achieve optimal processing and sealing performance.

[0010] Furthermore, the receiving cavity is provided with a viewing window to observe whether the plasma sample in the receiving cavity is full.

[0011] Furthermore, the cavity is transparent and visible to facilitate subsequent testing, such as when using optical detection methods to test plasma samples.

[0012] Another aspect of this utility model provides a plasma separation device, including a syringe barrel, an interface at one end of the syringe barrel, and a needle that is detachably and sealingly connected to the interface. The device is characterized in that it further includes the aforementioned whole blood separation device, wherein the first port of the whole blood separation device can be sealed and connected to the interface after the needle is removed.

[0013] This utility model has the following beneficial effects: The whole blood separation device provided by this invention allows for the separation of a plasma from a syringe after blood collection. The needle, detachably and sealingly connected to one end of the syringe barrel, is then transferred to the whole blood separation device. This eliminates the need for sample transfer and additional pressurization components; pressurization is achieved using the syringe piston assembly. The whole blood separation device filters the whole blood sample to obtain microliters of plasma. This method reduces the number of steps and shortens the separation time, thus improving detection efficiency. The whole blood separation device has a simple structure and can achieve quantitative separation of plasma under the combined action of air pressure and capillary force, resulting in excellent separation performance. Attached Figure Description

[0014] Figure 1 This is an exploded schematic diagram of a plasma separation device provided in some embodiments of this utility model. Figure 2 This is a schematic diagram of a plasma separation device provided in some embodiments of this utility model. Figure 3 This is a front view of the first cylinder in some embodiments of the whole blood separation device provided by this utility model. Figure 4 This is a front view of the second cylinder and the receiving cavity in some embodiments of the whole blood separation device provided by this utility model. Figure 5 yes Figure 4 Sectional view of the middle BB plane. Figure 6 This is a top view of the second cylinder and the receiving cavity in some embodiments of the whole blood separation device provided by this utility model.

[0015] Explanation of reference numerals in the attached figures: 001 Syringe Tube 100 First cylinder, 110 First port, 120 Second port, 130 First cavity. 200 Second cylinder, 210 Third port, 220 Fourth port, 230 Second cavity. 300 blood filter membrane, 400 contains the cavity, 401 is the viewing window. Detailed Implementation

[0016] To more clearly and completely describe the technical solution of this utility model, the following will describe the utility model in detail with reference to the accompanying drawings and embodiments. It should be noted that, unless otherwise specified, the embodiments and features in the embodiments of this utility model can be combined with each other. For ease of description, the terms "upper," "lower," "left," "right," "top," and "bottom" appearing below only indicate that the direction is consistent with the direction shown in the accompanying drawings and do not limit the structure.

[0017] Furthermore, it should be noted that in the description of this utility model, unless otherwise explicitly specified and limited, the terms "set," "connect," and "install" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0018] Example 1 This invention provides a plasma separation device for collecting whole blood samples and separating plasma samples from whole blood samples, such as... Figure 1 As shown, one end of the syringe barrel 001 is provided with a protruding interface and is equipped with a detachable needle (not shown in the figure). The needle is detachably and sealingly connected to the protruding interface. It is also equipped with the whole blood separation device provided by this utility model. The plasma separation device has a first connection type and a second connection type: In the first connection configuration, the needle is sealed to the protruding interface at one end of the syringe barrel 001; this first connection configuration is a common syringe connection configuration and can be used to collect blood samples from veins. In the second connection form, such as Figure 2 As shown, the first port 110 is axially sleeved on the outer periphery of the protruding interface and is snapped and sealed with the protruding interface.

[0019] After completing venous blood collection, the needle is removed, and the whole blood separation device provided by this invention is connected to a second connection configuration. The first port 110 of the whole blood separation device is sealed to the interface. The piston assembly of the syringe is pushed to push the whole blood sample into the whole blood separation device, which then separates plasma samples from the collected blood. The plasma separation process utilizes the piston assembly of the syringe to provide pressure, allowing for continuous filtration and separation of blood samples and plasma without transferring the sample to other pressurizing devices. The operation is simple and convenient.

[0020] like Figure 1 As shown, the whole blood separation device provided by this utility model includes a first cylinder 100, a second cylinder 200, a blood filtration membrane 300, and a receiving cavity 400; as Figure 3 As shown, the first cylindrical body 100 includes a first port 110, a second port 120, and a first cavity 130. The first port 110 and the second port 120 are arranged opposite to each other and are respectively connected to the first cavity 130; Figure 4 , Figure 5 and Figure 6 As shown, the second cylindrical body 200 includes a third port 210, a fourth port 220, and a second cavity 230. The third port 210 and the fourth port 220 are arranged opposite to each other and are respectively connected to the second cavity 230; as Figure 1 , Figure 4 and Figure 5As shown, the third port 210 is axially fitted around the outer periphery of the second port 120 and snaps shut with the second port. The periphery of the second port 120 can be rounded to facilitate smooth connection between the second port 120 and the third port 210. The blood filtration membrane 300 is placed at the bottom of the second cavity 230 and completely covers the fourth port 220. The second port 120 and the blood filtration membrane 300 abut against each other, and the fourth port 220 communicates with the receiving cavity 400. Optionally, the hollow volume of the receiving cavity 400 is 8-20 μl to facilitate quantitative separation of plasma.

[0021] The receiving cavity 400 can be selected in different shapes, such as cylinder, sphere or prism. The spherical shape of the receiving cavity has the best sealing performance. The second cylinder 200 can also be cylindrical or prism shaped and coaxially connected with the cylindrical or prism shaped receiving cavity, and the shape is connected, which is conducive to integral processing.

[0022] The whole blood separation device provided by this utility model has a simple structure. The first cylinder 100 and the second cylinder 200 are connected along the same axis, and the pressurization channel is unobstructed. The blood filtration membrane 300 is placed on the port connection surface where the two cylinders are connected, and the blood filtration membrane 300 is covered and pressed tightly on the fourth port 220 for plasma outflow by the second port 120 on the first cylinder 100, so as to avoid whole blood leakage and achieve good plasma filtration effect.

[0023] Example 2 like Figure 4 , Figure 5 and Figure 6 As shown, the receiving cavity is a hollow cylinder. The fourth port 220 connects to the receiving cavity 400 on the side circumference of the cylinder. The circular BB cross-section of the receiving cavity achieves excellent machining and sealing performance; as shown... Figure 4 As shown, the hollow width h of the cylinder can be selected in the range of 0.3-0.5mm to avoid the hollow width of the receiving cavity being too large, which would affect the capillary force of the receiving cavity, and too small, which would increase the processing difficulty of the device.

[0024] Example 3 like Figure 4 As shown, the receiving cavity 400 is provided with a viewing window 401 to observe whether the plasma sample in the receiving cavity is full.

[0025] Example 4 The receiving cavity 400 is made of transparent polymer material such as PP or PC. The transparent receiving cavity 400 is visible and facilitates subsequent testing. For example, when using optical detection methods to test plasma samples, the plasma in the transparent receiving cavity 400 can be directly tested, simplifying the testing process.

[0026] The whole blood separation device provided by this utility model has been described in detail above. Specific examples have been used to illustrate the principle and implementation of this utility model. The descriptions of the embodiments above are only for the purpose of helping to understand the core idea of ​​this utility model. It should be noted that those skilled in the art can make several improvements and modifications to this utility model without departing from the principle of this utility model, and these improvements and modifications also fall within the protection scope of the claims of this utility model.

Claims

1. A whole blood separation device, comprising a first cylinder (100), a second cylinder (200), a blood filtration membrane (300), and a receiving cavity (400), characterized in that, The first cylindrical body (100) includes a first port (110), a second port (120) and a first cavity (130), wherein the first port (110) and the second port (120) are arranged opposite to each other and are respectively connected to the first cavity (130); The second cylinder (200) includes a third port (210), a fourth port (220), and a second cavity (230). The third port (210) and the fourth port (220) are arranged opposite to each other and are respectively connected to the second cavity (230). The third port (210) is axially sleeved on the outer periphery of the second port (120) and snapped and sealed with the second port. The blood filtration membrane (300) is placed at the bottom of the second cavity (230) and covers the fourth port (220). The second port (120) and the blood filtration membrane (300) abut against each other. The fourth port (220) is connected to the receiving cavity (400).

2. The whole blood separation device according to claim 1, characterized in that, The second port (120) has rounded corners around its perimeter.

3. The whole blood separation device according to claim 1, characterized in that, The hollow volume of the accommodating cavity (400) is 8-20 μl.

4. The whole blood separation device according to claim 1, characterized in that, The hollow width h of the receiving cavity (400) is 0.3-0.5 mm.

5. The whole blood separation device according to claim 1, characterized in that, The receiving cavity (400) is a hollow cylinder, and the fourth port (220) is connected to the receiving cavity (400) on the side circumference of the cylinder.

6. The whole blood separation device according to claim 1, characterized in that, The receiving cavity (400) is provided with a viewing window.

7. The whole blood separation device according to claim 1, characterized in that, The receiving cavity (400) is transparent and visible.

8. A plasma separation device, comprising a syringe barrel (001), an interface at one end of the syringe barrel, and a needle detachably and sealingly connected to the interface, characterized in that, It also includes the whole blood separation device according to any one of claims 1-7, wherein the first port (110) of the whole blood separation device can be sealed to the interface after the needle is removed.

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