Chip sample injection filtering device

Abstract

The utility model discloses a kind of chip sample introduction filtering devices, belong to microfluidic chip technical field.It includes: sample introduction container, one side is provided with air pressure inlet;Outflow pipe, one end is inserted into the bottom of sample introduction container, the other end is worn out sample introduction container;Filtering component, is installed in the one end of outflow pipe;From air pressure inlet into compressed gas, promote the liquid in sample introduction container to pass through filtering component, from outflow pipe and flow out.Let the other end of outflow pipe connect microfluidic chip, compressed gas promotes sample liquid in sample introduction container to pass through filtering component's filtration from the one end of outflow pipe, from the other end of outflow pipe and flow out and enter microfluidic chip.Sample liquid in the bottom of sample introduction container is little, sample liquid in outflow pipe can also be pushed into microfluidic chip under the promotion of compressed gas, make the dead volume produced after filtration processing very small, reduce the waste of sample liquid, in addition, it also has the advantages such as low filtering cost, pipeline compatibility is strong and convenient to install operation.

Description

Technical Field

[0001] This utility model relates to the field of microfluidic chip technology, and in particular to a chip sample introduction and filtering device. Background Technology

[0002] In practical biochemical applications or scientific research using microfluidics, clogging is often a significant factor contributing to the failure of commercial products or scientific experiments. Existing common filtration methods include syringe-type filter heads, cell filter pretreatment, and channel-integrated filter structures, but these methods suffer from the drawback of excessive dead volume. Utility Model Content

[0003] The purpose of this invention is to provide a chip sample filtering device to solve one or more technical problems existing in the prior art, and at least provide a beneficial option or create conditions.

[0004] The technical solution adopted to solve the above-mentioned technical problems is as follows: a chip sample injection and filtration device, comprising: a sample injection container with a pressure inlet on one side; an outlet tube, one end of which extends into the bottom of the sample injection container and the other end of which extends out of the sample injection container; a filter assembly installed at the end of the outlet tube that extends into the sample injection container; compressed gas is introduced from the pressure inlet to push the liquid in the sample injection container through the filter assembly and then flow from one end of the outlet tube to the other end.

[0005] This technical solution has at least the following beneficial effects: the other end of the outflow tube is connected to a microfluidic chip. Compressed gas pushes the sample liquid in the injection container from one end of the outflow tube, and after being filtered by the filter assembly, it flows out from the other end of the outflow tube and enters the microfluidic chip, thus achieving sample liquid filtration. At the same time, there is less residual sample liquid at the bottom of the injection container, and the sample liquid in the outflow tube can also enter the microfluidic chip under the push of compressed gas, so that the dead volume generated after filtration is very small, reducing sample liquid waste. In addition, it also has the advantages of low filtration cost, strong pipeline compatibility, and easy installation and operation.

[0006] As a further improvement to the above technical solution, the filtration assembly includes a biofilter membrane sleeved on one end of the outflow tube that extends into the sample inlet container.

[0007] As a further improvement to the above technical solution, the filtration assembly also includes an inner nested tube sleeved on one end of the outflow tube that extends into the sample inlet container, and the biofilter membrane is sleeved on the inner nested tube.

[0008] As a further improvement to the above technical solution, the filtration assembly also includes an outer nested tube sleeved outside the inner nested tube. One end of the outer nested tube has a flow port corresponding to the outlet tube port. The width of the flow port is smaller than the outer diameter of the inner nested tube, so that an inner bottom wall is formed inside the outer nested tube. The end face of the inner nested tube and the inner bottom wall of the outer nested tube together clamp the bio-filter membrane.

[0009] As a further improvement to the above technical solution, the edge of the biofilter membrane is sandwiched between the outer wall of the inner nested tube and the inner wall of the outer nested tube.

[0010] As a further improvement to the above technical solution, the edge of the biofilter membrane extends beyond the outer nested tube.

[0011] As a further improvement to the above technical solution, the inner nested tube is connected to an inlet tube, and the biofilter membrane is sandwiched between the inlet tube and the outlet tube.

[0012] As a further improvement to the above technical solution, the bottom of the injection container is conical.

[0013] As a further improvement to the above technical solution, the sample injection container includes a sample liquid tube and a sealing cap covering the opening of the sample liquid tube. The gas pressure inlet is located on the sealing cap, and the other end of the outflow tube extends out of the sealing cap. An air inlet pipe is installed at the gas pressure inlet of the sealing cap.

[0014] As a further improvement to the above technical solution, a connecting column is provided at the top of the injection container, and the other end of the outflow tube is sealed through the connecting column. Attached Figure Description

[0015] The above and / or additional aspects and advantages of this utility model will become apparent and readily understood from the description of the embodiments taken in conjunction with the following drawings, in which: Figure 1 This is a schematic diagram of the overall structure of an embodiment of the present utility model; Figure 2 This is a schematic diagram of the structure of the filter assembly in an embodiment of the present invention; Figure 3 This is a schematic diagram of the structure of the biofiltration membrane in an embodiment of this utility model; Figure 4 This is a schematic diagram of the specific structure of the sample injection container in an embodiment of this utility model.

[0016] 100. Sample inlet container; 110. Sample tube; 120. Sealing cap; 121. Gas pressure inlet; 122. Gas inlet tube; 130. Connecting column; 200. Outlet tube; 300. Filter assembly; 310. Biofiltration membrane; 320. Inner nested tube; 330. Outer nested tube; 331. Flow port. Detailed Implementation

[0017] The embodiments of this utility model are described in detail below. Examples of these embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain this utility model, and should not be construed as limiting this utility model.

[0018] In the description of this utility model, it should be understood that the directional descriptions, such as up, down, front, back, left, right, etc., indicate the directional or positional relationship based on the directional or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

[0019] In the description of this utility model, "several" means one or more, "multiple" means two or more, "greater than," "less than," and "exceeding" are understood to exclude the stated number, while "above," "below," and "within" are understood to include the stated number. If "first" or "second" is used in the description, it is only for the purpose of distinguishing technical features and should not be construed as indicating or implying relative importance, or implicitly indicating the number of indicated technical features, or implicitly indicating the order of the indicated technical features.

[0020] In the description of this utility model, unless otherwise explicitly defined, terms such as "setting," "installation," and "connection" should be interpreted broadly, and those skilled in the art can reasonably determine the specific meaning of the above terms in this utility model in conjunction with the specific content of the technical solution.

[0021] Reference Figure 1-4 A chip sample introduction and filtration device includes a sample introduction container 100, an outflow tube 200, and a filter assembly 300.

[0022] The sample inlet container 100 includes a sample tube 110 and a sealing cap 120. The sample tube 110 is used to hold the sample solution. The sample tube 110 can be a disposable reaction tube with a cap on one side of its top. After opening the cap, the sealing cap 120 is sealed to the top opening of the reaction tube, creating a sealed space between the sealing cap 120 and the sample tube 110. The sealing cap 120 and the sample tube 110 can be connected by threaded connection, bolt connection, snap-fit ​​connection, or interference fit. The bottom of the sample tube 110 is tapered with a gradually decreasing area to minimize the amount of sample solution remaining inside. The reaction tube is generally made of a transparent material to facilitate observation of the remaining sample solution.

[0023] The sealing cover 120 has a pressure inlet 121 on one side that connects to the internal sealed space. An air inlet pipe 122 is installed on the sealing cover 120 at the pressure inlet 121. The air inlet pipe 122 is used to connect to a compressed gas source. The sealing cover 120 is made of plastic, and the air inlet pipe 122 is made of metal.

[0024] A vertically arranged connecting post 130 is provided on the top surface of the sealing cap 120. One end of the outflow tube 200 extends into the bottom of the sample tube 110, and the other end passes through the connecting post 130 of the sealing cap 120. The outflow tube 200 and the connecting post 130 are sealed together, such as by providing a sealing ring between them. It is understood that the outflow tube 200 can slide relative to the connecting post 130 and the sealing cap 120, thereby adjusting the length of the outflow tube 200 extending into the sample tube 110. In other embodiments, the sample tube 110 and the sealing cap 120 can also be made into an integral structure, and the sample is pre-injected into the sample inlet container 100.

[0025] The filter assembly 300 includes a biofiltration membrane 310, an inner nested tube 320, and an outer nested tube 330.

[0026] The inner nested tube 320 is fitted onto the end of the outflow tube 200 that extends into the bottom of the sample tube 110. The outflow tube 200 and the inner nested tube 320 can be connected by an interference fit, or by the elasticity and friction between the materials to achieve a tight fit. The biofilter membrane 310 is fitted onto the end opening of the inner nested tube 320 near the bottom of the sample tube 110, thereby covering the end opening of the outflow tube 200 that extends into the bottom of the sample tube 110, so that the sample liquid must be filtered by the biofilter membrane 310 before entering the outflow tube 200.

[0027] One end of the outer nested tube 330 is into which the inner nested tube 320 is embedded. The inner nested tube 320 and the outer nested tube 330 can also be tightly connected by an interference fit. The other end of the outer nested tube 330 is closed and has a flow port 331, which connects to the interior of the outer nested tube 330 and is arranged correspondingly to the inner channel of the outflow tube 200. The size of the outer nested tube 330 is between 1-10 mm. The size of the biofilter membrane 310 is between 1 nm and 1 mm. The size of the biofilter membrane 310 is selected according to actual needs. For conventional biochemical applications, the size is designed to be between 1-100 μm.

[0028] The width of the flow port 331 is smaller than the inner diameter of the inner nested tube 320, so that the inner bottom wall of the outer nested tube 330 is formed. The end of the inner nested tube 320 and the inner bottom wall of the outer nested tube 330 together clamp the biofilter membrane 310, maintaining the installation stability of the biofilter membrane 310.

[0029] The width of the biofilter membrane 310 is greater than the end face width of the inner nested tube 320, so that the edge of the biofilter membrane 310 is clamped between the outer wall of the inner nested tube 320 and the inner wall of the outer nested tube 330, thereby improving the installation stability of the biofilter membrane 310. It can be understood that the outer diameter of the inner nested tube 320 is slightly smaller than the inner diameter of the outer nested tube 330, and the gap between the outer wall of the inner nested tube 320 and the inner wall of the outer nested tube 330 is smaller than the thickness of the biofilter membrane 310.

[0030] Furthermore, the width of the biofilter membrane 310 minus the end face width of the inner nested tube 320 is greater than twice the inner bottom wall depth of the outer nested tube 330, so that the edge of the biofilter membrane 310 extends beyond the outer nested tube 330, facilitating the installation, disassembly, and replacement of the biofilter membrane 310. The length of the inner nested tube 320 is greater than the inner bottom wall depth of the outer nested tube 330, facilitating the installation, disassembly, and replacement of the inner nested tube 320 and the outer nested tube 330.

[0031] Both the inner nested tube 320 and the outer nested tube 330 can be formed by 3D printing, machining or micro-injection molding.

[0032] In other embodiments, the filter assembly 300 may omit the inner nested tube 320 and the outer nested tube 330, and the biofilter membrane 310 may be directly fitted onto the end of the outflow tube 200 that extends into the bottom wall of the sample tube 110 and then secured. In other embodiments, the filter assembly 300 may omit the outer nested tube 330, and the biofilter membrane 310 may be directly fitted onto the end of the inner nested tube 320 and then secured.

[0033] In another embodiment, the outer nested tube 330 or the inner nested tube 320 may be connected to an inlet tube, which together with the inner nested tube 320 clamps the biofilter membrane 310, or the inlet tube and the outlet tube 200 together clamp the biofilter membrane 310. The sample solution entering from the inlet tube is filtered by the biofilter membrane 310 and then enters the outlet tube 200, thus achieving sample solution filtration. This embodiment can not only filter the liquid in the sample solution tube 110, but also filter the liquid between any two tubes.

[0034] When it is necessary to filter the sample solution before injecting it into the microfluidic chip, select a suitable biofilter membrane 310 and assemble the filter assembly 300. Seal the reaction tube containing the sample solution to the sealing cap 120. One end of the outflow tube 200 containing the filter assembly 300 enters the bottom of the reaction tube, and the other end of the outflow tube 200 extends from the connecting post 130 of the sealing cap 120 and connects to the sample inlet end of the microfluidic chip. Adjust the position of the outflow tube 200. Compressed gas is introduced into the reaction tube through the air inlet tube 122. The compressed gas pushes the sample solution through the biofilter membrane 310 and into the outflow tube 200. The filtered sample solution enters the microfluidic chip through the outflow tube 200, thus realizing the filtration treatment of the sample solution.

[0035] Minimal sample residue remains at the bottom of the reaction tube, and the sample in the outflow tube 200 can also enter the microfluidic chip under the propulsion of compressed gas, resulting in a very small dead volume after filtration and reducing sample waste. Furthermore, it offers advantages such as low filtration cost, strong pipeline compatibility, and ease of installation and operation.

[0036] The embodiments of the present utility model have been described in detail above with reference to the accompanying drawings. However, the present utility model is not limited to the above embodiments. Within the scope of knowledge possessed by those skilled in the art, various changes can be made without departing from the spirit of the present utility model.

Claims

1. A chip sample introduction and filtering device, characterized in that, include: The sample inlet container has a pressure inlet on one side; The outflow tube has one end inserted into the bottom of the injection container and the other end protruding out of the injection container; A filter assembly is installed at one end of the outflow tube that extends into the sample inlet container; compressed gas is introduced from the gas pressure inlet, which pushes the liquid in the sample inlet container through the filter assembly and then flows from one end of the outflow tube to the other end.

2. The chip sample introduction and filtering device according to claim 1, characterized in that: The filtration assembly includes a biofilter membrane fitted over the end of the outflow tube that extends into the sample inlet container.

3. The chip sample introduction and filtering device according to claim 2, characterized in that: The filtration assembly also includes an inner nested tube sleeved on one end of the outflow tube that extends into the sample inlet container, and the biofilter membrane is sleeved on the inner nested tube.

4. The chip sample introduction and filtering device according to claim 3, characterized in that: The filtration assembly also includes an outer nested tube sleeved outside the inner nested tube. One end of the outer nested tube has a flow port corresponding to the outlet port. The width of the flow port is smaller than the outer diameter of the inner nested tube, so that an inner bottom wall is formed inside the outer nested tube. The end face of the inner nested tube and the inner bottom wall of the outer nested tube together hold the biofilter membrane.

5. The chip sample introduction and filtering device according to claim 4, characterized in that: The edge of the biofiltration membrane is sandwiched between the outer wall of the inner nested tube and the inner wall of the outer nested tube.

6. The chip sample introduction and filtering device according to claim 5, characterized in that: The edge of the biofiltration membrane extends beyond the outer nested tube.

7. The chip sample introduction and filtering device according to claim 3, characterized in that: The inner nested tube is connected to an inlet tube, and the biofilter membrane is sandwiched between the inlet tube and the outlet tube.

8. The chip sample introduction and filtering device according to claim 1, characterized in that: The bottom of the sample inlet container is conical.

9. The chip sample introduction and filtering device according to claim 1, characterized in that: The sample inlet container includes a sample tube and a sealing cap covering the opening of the sample tube. The pressure inlet is located on the sealing cap, and the other end of the outflow tube extends out of the sealing cap. An air inlet pipe is installed at the pressure inlet of the sealing cap.

10. The chip sample introduction and filtering device according to claim 1, characterized in that: The top of the injection container is provided with a connecting column, and the other end of the outflow tube is sealed through the connecting column.

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