A methylation detection reagent preparation device

Abstract

The utility model relates to biological reagent preparation technical field relates to a methylation detection reagent preparation device, including sealed cavity, the upper end of sealed cavity is installed with a plurality of storage tank, install through setting independent feed channel between storage tank and sealed cavity, install in the sealed cavity and have multifunctional stirring assembly, multifunctional stirring assembly include sealed cavity rotation connection's stirring axle, the helical dispersion paddle of edge circumferential rotation is installed to the stirring axle upper end, the oblique shearing paddle is installed to the stirring axle lower extreme, the wall scraping paddle is installed to the oblique shearing paddle's end, effectively solved the problem of low raw material proportioning accuracy and poor mixing efficiency in the prior art.

Description

Technical Field

[0001] This utility model relates to the field of biological reagent preparation technology, and in particular to a methylation detection reagent preparation device. Background Technology

[0002] Methylation detection is an important diagnostic tool in the field of precision medicine, and the quality of reagent preparation directly affects the accuracy of test results. Current methylation detection reagent preparation processes suffer from the following problems: First, the raw material ratio accuracy is low. The enzymes, buffer solutions, primers, and probes required for methylation detection reagents must be mixed in strict proportions. Traditional manual feeding or single-channel feeding methods are prone to dosage deviations, leading to unstable reagent activity. Second, the mixing efficiency is poor. Different raw materials have significant differences in solubility and density, and conventional stirring devices are prone to producing uneven local concentrations, affecting reagent reaction efficiency. Utility Model Content

[0003] This invention addresses the shortcomings of existing technologies by providing a methylation detection reagent preparation device that effectively solves the problems of low raw material ratio accuracy and poor mixing efficiency in existing technologies.

[0004] The technical solution adopted by this utility model to solve the above problems is as follows: A methylation detection reagent preparation device includes a sealed cavity. Several storage tanks are installed at the upper end of the sealed cavity. The storage tanks and the sealed cavity are connected by an independent feeding channel. A multifunctional stirring assembly is installed inside the sealed cavity. The multifunctional stirring assembly includes a stirring shaft rotatably connected to the sealed cavity. A spiral dispersing paddle that rotates on its own axis and in a circular motion is installed at the upper end of the stirring shaft. An oblique shearing paddle is installed at the lower end of the stirring shaft. A wall scraping paddle is installed at the end of the oblique shearing paddle.

[0005] Preferably, the independent feed channel includes a plunger pump installed in the storage tank pipeline, and the outlet pipeline of the plunger pump is equipped with an anti-backflow valve body.

[0006] Preferably, the blades of the spiral dispersing paddle have a spiral structure, the fixed cylinder is fixedly installed at the upper end of the sealed cavity, and a fixed bevel gear is fixedly installed at the lower end of the fixed cylinder. Both the fixed bevel gear and the rotating bevel gear are installed in a protective box that is fixedly connected to the stirring shaft. The two ends of the fixed bevel gear are respectively meshed with rotating bevel gears that are rotatably connected to the protective box.

[0007] Preferably, the blades of the oblique shearing paddle are set at a preset angle to the stirring shaft.

[0008] Preferably, the scraper is made of an elastic material, which is silicone.

[0009] Preferably, the fixing plate has mounting holes adapted to the shape of the storage tank, and the storage tank is detachably connected to the fixing plate through the mounting holes.

[0010] Preferably, the inner wall of the sealed cavity is provided with a corrosion-resistant coating, which is a ceramic coating.

[0011] Compared with the prior art, this utility model has the following advantages: 1. The design incorporates several storage tanks with corresponding independent feed channels, allowing for the separate storage and transport of different raw materials such as enzymes, buffer solutions, and primers / probes. This avoids cross-contamination or dosage interference associated with single-channel feeding. The plunger pumps connected in series within the independent feed channels precisely control the feed rate, while the anti-backflow valve prevents backflow deviation. This design completely replaces the subjective errors of traditional manual feeding and the dosage fluctuations associated with single-channel feeding, ensuring that each raw material is strictly proportioned according to the formula. This directly guarantees the activity and stability of the methylation detection reagent and reduces reagent waste caused by proportioning deviations.

[0012] 2. The spiral dispersion paddle at the upper end of the stirring shaft adopts a spiral structure that rotates on its own axis while also rotating in a circular motion. This structure can initially disperse the raw materials that have just entered the sealed cavity, quickly breaking down the stratification of raw materials with different densities and solubilities, such as low-density primers and probes and high-density buffer solutions, laying the foundation for subsequent mixing. The inclined shearing paddle at the lower end, through the design of the blades and the stirring shaft at a preset angle, can generate local turbulence when rotating at high speed. This high-intensity shearing of poorly soluble raw materials, such as enzyme preparations, accelerates their dissolution and dispersion, preventing excessively high local concentrations. The synergy of these two components can significantly shorten mixing time, improve mixing efficiency, and solve the problems of incomplete mixing and long mixing times associated with conventional stirring. The silicone wall scraper at the end of the inclined shearing paddle can rotate closely against the inner wall of the sealed cavity, scraping off the raw materials adhering to the inner wall and bringing them back to the mixing system. This avoids waste caused by raw materials adhering to the inner wall and prevents residual raw materials from deteriorating due to prolonged residence. At the same time, it ensures that there are no dead zones in the cavity, further improving mixing uniformity and ensuring consistent reaction efficiency in all parts of the reagent. Attached Figure Description

[0013] Figure 1 This is an isometric view of a methylation detection reagent preparation device according to the present invention; Figure 2 This is a schematic diagram of the internal structure of the sealed cavity of a methylation detection reagent preparation device according to the present invention; Figure 3 This is a schematic diagram of the spiral dispersion paddle of a methylation detection reagent preparation device according to the present invention; Figure 4 This is a schematic diagram of the storage tank of a methylation detection reagent preparation device according to the present invention; Figure 5This is a schematic diagram of the wall scraping paddle of a methylation detection reagent preparation device according to the present invention; The following are the labels in the diagram: 1-Sealed cavity, 2-Discharge pipe, 3-Regulating valve, 4-Fixed plate, 5-Storage tank, 6-Anti-backflow valve body, 7-Wall scraper, 8-Protective box, 9-Spiral dispersing impeller, 10-Fixed bevel gear, 11-Angled shearing impeller, 12-Rotating bevel gear, 13-Fixed cylinder, 14-Agitator shaft, 15-Plunger pump. Detailed Implementation

[0014] The following are specific embodiments of the present invention, and the technical solution of the present invention will be further described in conjunction with the accompanying drawings. However, the present invention is not limited to these embodiments.

[0015] like Figure 1-5 As shown, a methylation detection reagent preparation device includes a sealed cavity 1. Several storage tanks 5 are installed at the upper end of the sealed cavity 1. The storage tanks 5 and the sealed cavity 1 are connected by an independent feeding channel. A multifunctional stirring assembly is installed inside the sealed cavity 1. The multifunctional stirring assembly includes a stirring shaft 14 rotatably connected to the sealed cavity 1. A spiral dispersing paddle 9 that rotates on its own axis and rotates in a circular motion is installed at the upper end of the stirring shaft 14. An oblique shearing paddle 11 is installed at the lower end of the stirring shaft 14. A wall scraping paddle 7 is installed at the end of the oblique shearing paddle 11.

[0016] The independent feed channel includes a plunger pump 15 installed in the pipeline of the storage tank 5, and the outlet pipeline of the plunger pump 15 is equipped with an anti-backflow valve body 6.

[0017] like Figure 2 and 4 As shown, the plunger pumps 15 of each independent feeding channel start synchronously. Driven by a motor, the plunger pumps 15 draw raw materials from the corresponding storage tanks 5 according to the preset dosage. After being quantitatively processed by the plunger pumps 15, the raw materials flow along the pipeline to the anti-backflow valve body 6. This valve body is a one-way valve, which only allows the raw materials to flow from the storage tank 5-plunger pump 15-sealed cavity 1. This effectively avoids the raw materials from flowing back to the plunger pump 15 or storage tank 5 due to pressure fluctuations in the cavity during subsequent mixing, such as the instantaneous pressure generated by stirring, thus preventing cross-contamination of raw materials and deviations in the mixing ratio.

[0018] The blades of the spiral dispersing paddle 9 are spiral in shape. The fixed cylinder 13 is fixedly installed at the upper end of the sealed cavity 1. A fixed bevel gear 10 is fixedly installed at the lower end of the fixed cylinder 13. The fixed bevel gear 10 and the rotating bevel gear 12 are both installed in the protective box 8 which is fixedly connected to the stirring shaft 14. The two ends of the fixed bevel gear 10 are respectively meshed with the rotating bevel gear 12 which is rotatably connected to the protective box 8.

[0019] The blades of the oblique shearing paddle 11 are set at a preset angle to the stirring shaft 14.

[0020] The wall scraper 7 is made of an elastic material, namely silicone.

[0021] like Figure 2 and 3 As shown in Figure 5, the motor drives the stirring shaft 14 to rotate. While rotating in a circular motion with the stirring shaft 14, the rotating shaft 14, along with the rotation of its own spiral blades, is protected by the fixed cylinder 13, which is fixedly installed at the upper end of the sealed cavity 1. The fixed cylinder 13 secures the fixed bevel gear 10. The fixed bevel gear 10 and the rotating bevel gear 12 are installed inside the protective box 8, which protects them. As the protective box 8 rotates with the stirring shaft 14, it is rotatably mounted with the fixed cylinder 13 and the fixed bevel gear 10. Therefore, the protective box 8 drives the rotating bevel gear 12 to rotate in a circular motion, which meshes with the fixed bevel gear 10, thus achieving simultaneous circular and self-rotation of the rotating bevel gear 12. The rotating bevel gear 12 drives the spiral dispersion paddle 9 to rotate in a circular motion while also rotating on its own axis, initially dispersing the raw materials that have just entered the mixing zone. The raw materials are quickly broken up by the spiral push and shearing action of the spiral dispersion paddle 9, achieving initial mixing. The blades are at a preset angle to the stirring shaft 14 and rotate at high speed with the stirring shaft 14, performing high-intensity shearing on the initially dispersed raw materials. For enzyme preparations with poor solubility, the oblique shearing paddle 11 can generate local turbulence, accelerating the dissolution and dispersion of the enzyme preparation and avoiding the problem of excessively high local concentrations. Made of silicone elastic material, it tightly adheres to the inner wall of the sealed cavity 1 when rotating synchronously with the oblique shearing paddle 11, scraping off the raw materials adhering to the inner wall and bringing them back to the mixing system. This avoids waste of raw materials and prevents the residual raw materials on the inner wall from deteriorating due to prolonged residence, thus affecting the quality of the reagents.

[0022] The fixing plate 4 has mounting holes that are adapted to the shape of the storage tank 5, and the storage tank 5 is detachably connected to the fixing plate 4 through the mounting holes.

[0023] like Figure 1 and 2 As shown, each storage tank 5 can be detachably fixed via the adapter mounting holes on the fixing plate 4, which facilitates the replenishment of raw materials and cleaning of the tank.

[0024] The inner wall of the sealed cavity 1 is provided with a corrosion-resistant coating, which is a ceramic coating.

[0025] like Figure 2 As shown, ceramic corrosion-resistant coatings can reduce the adsorption of raw materials and the adhesion of microorganisms, further reducing the risk of pollution.

[0026] The working process of this utility model is as follows: According to the formula requirements of the methylation detection reagent, the operator puts different raw materials such as enzyme preparation, buffer solution, primer probe and so on into the storage tank 5 with corresponding independent feeding channel. Each storage tank 5 can be detached and fixed by the adapter mounting hole on the fixing plate 4, which facilitates the replenishment of raw materials and cleaning of the tank.

[0027] The plunger pumps 15 of each independent feeding channel start synchronously. Driven by a motor, the plunger pumps 15 draw raw materials from the corresponding storage tanks 5 according to the preset dosage. After being metered by the plunger pumps 15, the raw materials flow along the pipeline to the anti-backflow valve body 6. This valve body is a one-way valve, which only allows the raw materials to flow from the storage tank 5-plunger pump 15-sealed cavity 1. This effectively avoids the raw materials from flowing back to the plunger pump 15 or storage tank 5 due to pressure fluctuations in the cavity during subsequent mixing, such as the instantaneous pressure generated by stirring. This prevents cross-contamination of raw materials and deviations in the proportioning.

[0028] After passing through the anti-backflow valve body 6, the raw materials from each channel simultaneously enter the mixing zone of the sealed cavity 1. Once in the mixing zone, the motor drives the stirring shaft 14 to rotate. As the stirring shaft 14 rotates in a circular motion, the raw materials also rotate due to the rotation of their own spiral blades. Since the fixed cylinder 13 is fixedly installed at the upper end of the sealed cavity 1, it secures the fixed bevel gear 10. The fixed bevel gear 10 and the rotating bevel gear 12 are installed inside the protective box 8, which protects them. When the protective box 8 rotates with the stirring shaft 14, the fixed bevel gear 10 and the rotating bevel gear 12 are protected. The fixed cylinder 13 and the fixed bevel gear 10 are rotatably installed. Therefore, the protective box 8 drives the rotating bevel gear 12 to rotate in a circular motion, which meshes with the fixed bevel gear 10. This allows the rotating bevel gear 12 to rotate in a circular motion while also rotating on its own axis. The rotating bevel gear 12 drives the spiral dispersion paddle 9 to rotate in a circular motion while also rotating on its own axis, thus initially dispersing the raw materials that have just entered the mixing zone. The raw materials are pushed and sheared by the spiral dispersion paddle 9, which can quickly break up the material stratification and achieve initial mixing. The blades are at a preset angle to the stirring shaft 14 and rotate at high speed with the stirring shaft 14, performing high-intensity shearing on the initially dispersed raw materials. For enzyme preparations with poor solubility, the oblique shearing paddle 11 can generate local turbulence, accelerating the dissolution and dispersion of the enzyme preparation and avoiding the problem of excessively high local concentrations. It is made of silicone elastic material and, when rotating synchronously with the oblique shearing paddle 11, it fits tightly against the inner wall of the sealed cavity 1, scraping off the raw materials adhering to the inner wall and bringing them back to the mixing system. This avoids waste of raw materials and prevents the residual raw materials on the inner wall from deteriorating due to prolonged residence, thus affecting the quality of the reagents.

[0029] After mixing is complete, open the regulating valve 3 to discharge the raw material from the discharge pipe 2 into the sealed cavity 1.

[0030] After the material is discharged, close the regulating valve 3 and inject sterile water or special cleaning agent into the storage tank 5. The water is then pumped to the sealed cavity 1 by the plunger pump 15. The stirring shaft 14 rotates and works with the wall scraper 7 to clean the inner wall of the cavity and the pipes. The wastewater after cleaning is discharged through the discharge pipe 2.

[0031] The specific embodiments described herein are merely illustrative examples illustrating the spirit of this utility model. Those skilled in the art to which this utility model pertains may make various modifications or additions to the described specific embodiments or use similar methods to replace them, without departing from the spirit of this utility model or exceeding the scope defined by the appended claims.

Claims

1. A methylation detection reagent preparation apparatus, characterized in that: The device includes a sealed cavity (1), with several storage tanks (5) installed at the upper end of the sealed cavity (1). The storage tanks (5) and the sealed cavity (1) are connected by an independent feeding channel. A multi-functional stirring assembly is installed inside the sealed cavity (1). The multi-functional stirring assembly includes a stirring shaft (14) rotatably connected to the sealed cavity (1). A spiral dispersing paddle (9) that rotates on its own axis and rotates in a circular motion is installed at the upper end of the stirring shaft (14). An oblique shearing paddle (11) is installed at the lower end of the stirring shaft (14). A wall scraping paddle (7) is installed at the end of the oblique shearing paddle (11).

2. The methylation detection reagent preparation apparatus according to claim 1, characterized in that: The independent feed channel includes a plunger pump (15) installed in the pipeline of the storage tank (5), and the outlet pipeline of the plunger pump (15) is equipped with an anti-backflow valve body (6).

3. The methylation detection reagent preparation apparatus according to claim 1, characterized in that: The blades of the spiral dispersion paddle (9) are spiral in shape. The fixed cylinder (13) is fixedly installed at the upper end of the sealed cavity (1). The fixed bevel gear (10) is fixedly installed at the lower end of the fixed cylinder (13). The fixed bevel gear (10) and the rotating bevel gear (12) are both installed in the protective box (8) which is fixedly connected to the stirring shaft (14). The two ends of the fixed bevel gear (10) are respectively meshed with the rotating bevel gear (12) which is rotatably connected to the protective box (8).

4. The methylation detection reagent preparation apparatus according to claim 1, characterized in that: The blades of the oblique shearing paddle (11) are set at a preset angle to the stirring shaft (14).

5. The methylation detection reagent preparation apparatus according to claim 1, characterized in that: The scraper (7) is made of an elastic material, namely silicone.

6. The methylation detection reagent preparation apparatus according to claim 1, characterized in that: The fixing plate (4) has mounting holes that are adapted to the shape of the storage tank (5), and the storage tank (5) is detachably connected to the fixing plate (4) through the mounting holes.

7. The methylation detection reagent preparation apparatus according to claim 1, characterized in that: The inner wall of the sealed cavity (1) is provided with a corrosion-resistant coating, which is a ceramic coating.

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