A centrifugal cup

Abstract

The application discloses a centrifugal cup, and relates to the technical field of centrifugal devices. The application provides a centrifugal cup, which comprises a cup body having a containing groove for containing materials to be centrifugally separated; a cup cover arranged on the cup body and used for closing the containing groove, the cup cover having a liquid inlet channel and a liquid outlet channel which are spaced apart from each other, the liquid inlet channel being in communication with the containing groove, and a first liquid outlet end of the liquid outlet channel penetrating through the cup cover and being close to an inner wall of the containing groove; wherein the first liquid outlet end of the liquid outlet channel is connected with a rotatable flow guide disc, the flow guide disc has a flow guide channel in the inside of the flow guide disc and in communication with the liquid outlet channel, the flow guide disc is provided with flow guide holes which are in communication with the flow guide channel and the containing groove, and the flow guide holes are located at the periphery of the first liquid outlet end of the liquid outlet channel.

Description

Technical Field

[0001] This application relates to the field of centrifuge technology, and more particularly to a centrifuge cup. Background Technology

[0002] In cell processing, the cell fluid typically requires concentration, washing, separation, and purification. Centrifugation primarily utilizes the density difference of the liquids to separate the cells into layers through high-speed rotation. Because the components within the cells have different masses, the centrifugal forces acting on each component are also different, resulting in varying radii of rotation within the centrifuge vessel, thus allowing for the extraction of the desired cells. In existing technologies, the centrifuged fluid is discharged through an outlet channel. The distance between the outlet channel's opening and the center of rotation is fixed, but the rotation radius may differ depending on the type of cell fluid. When a different distance between the outlet channel's opening and the center of rotation is required, a centrifuge with a different centrifugal radius is usually used, increasing operating costs. Utility Model Content

[0003] The main objective of this application is to provide a centrifuge cup that addresses the technical problem in the prior art where, when the distance between the outlet of the liquid outlet and the center of rotation needs to be different, centrifuge devices with different centrifugal radii are usually replaced, increasing the cost of use.

[0004] To achieve the above objectives, this application provides a centrifuge cup, comprising: The cup body has a receiving groove for holding the material to be centrifuged and separated. A cup lid is disposed on the cup body and used to close the receiving groove. The cup lid has a liquid inlet channel and a liquid outlet channel that are separated from each other. The liquid inlet channel is connected to the receiving groove, and the first liquid outlet end of the liquid outlet channel penetrates the inner wall of the cup lid near the receiving groove. The liquid outlet channel has a first liquid outlet end connected to a rotatable guide plate. The guide plate has a guide channel inside that communicates with the liquid outlet channel. The guide plate is provided with a guide hole that communicates with the guide channel and the receiving groove. The guide hole is located on the periphery of the first liquid outlet end of the liquid outlet channel.

[0005] Optionally, the inner wall of the cup lid is provided with a positioning groove, the flow guide plate is disposed in the positioning groove, and a positioning sealing ring is provided between the outer wall of the flow guide plate and the inner wall of the positioning groove.

[0006] Optionally, the cross-sectional area of ​​the flow channel gradually decreases from the first liquid outlet end along the direction close to the flow guide hole.

[0007] Optionally, the guide plate has a connecting protrusion for connecting to the first liquid outlet end, and the connecting protrusion is interference-fitted with the first liquid outlet end.

[0008] Optionally, the cup lid is further provided with a dynamic sealing seat, the dynamic sealing seat having a flow hole communicating with the second liquid outlet end of the liquid outlet channel; A connector is provided above the cup lid, and the connector has a liquid inlet and a liquid outlet. The connector is provided with a first guide tube and a second guide tube. The second guide tube is sleeved around the first guide tube, and the two ends of the first guide tube protrude beyond the two ends of the second guide tube. The first guide tube passes through the dynamic sealing seat and connects the liquid inlet hole and the liquid inlet channel; A flow passage is formed between the inner wall of the second guide tube and the outer wall of the first guide tube, and the flow passage connects the liquid outlet and the flow passage.

[0009] Optionally, the cup lid has a fixing groove for installing the dynamic sealing seat, and a first sealing ring is provided between the outer wall of the dynamic sealing seat and the inner wall of the fixing groove, respectively located on the upper and lower sides of the flow hole.

[0010] Optionally, a bearing is provided on the dynamic seal seat, the second guide tube passes through the bearing, and a second sealing ring is provided between the second guide tube and the dynamic seal seat.

[0011] Optionally, a third sealing ring is provided between the portion of the lower end of the first guide tube that protrudes from the lower end of the second guide tube and the dynamic sealing seat.

[0012] Optionally, one end of the first guide tube that passes through the dynamic sealing seat is connected to a dynamic sealing support.

[0013] Optionally, the bottom of the cup body is provided with at least one radially extending drainage tube, the inner end of all the drainage tubes is connected to the liquid inlet channel, and the outer end of all the drainage tubes has a communication hole that communicates with the receiving groove.

[0014] The beneficial effects that this application can achieve are: The centrifuge cup proposed in this application embodiment forms a relatively sealed space through the cup body and cup lid. The cell fluid to be centrifuged can be transported to the container tank for centrifugation through the liquid inlet channel set on the cup lid. A guide plate is set at the channel opening of the liquid outlet channel of the cup lid, and a guide hole is set on the guide plate. The guide hole and the liquid outlet channel are interconnected through the guide channel. That is, as the cell fluid gradually enters the container tank for centrifugation, the liquid of the stratified cell fluid near the liquid outlet channel first enters the guide channel through the guide hole and then is gradually discharged through the liquid outlet channel. The guide hole is positioned off-center from the guide plate. When the guide plate is rotated, it rotates along its own axis, changing the position of the guide hole relative to the center line of the cup lid. This allows for adjustment of the distance between the guide hole and the center line of the cup lid, effectively changing the distance between the first liquid outlet end of the liquid outlet channel and the center line of the cup lid. This meets the user's needs for separating liquids with different centrifugal radii. Furthermore, the addition of the guide plate does not damage the structure of the cup lid itself, facilitating technical modifications based on existing cup lids and expanding the scope of application. Attached Figure Description

[0015] Figure 1 This is a cross-sectional structural diagram of the centrifuge cup according to an embodiment of this application; Figure 2 for Figure 1 Enlarged structural diagram at point A in the middle; Figure 3 This is an enlarged structural diagram of the dynamic sealing seat in an embodiment of this application; Figure 4 This is a schematic diagram of the exploded structure of a centrifuge cup according to an embodiment of this application.

[0016] The numbers on the map are: 10-Cup body, 11-Containing groove, 20-Cup lid, 21-Inlet channel, 22-Outlet channel, 23-Positioning groove, 30-Guide plate, 31-Connecting convex ring, 32-Guide channel, 33-Guide hole, 34-Positioning sealing ring, 40-Dynamic seal seat, 41-First sealing ring, 42-Second sealing ring, 43-Third sealing ring, 44-Bearing, 45-Dynamic seal support, 50-Connector, 51-Inlet hole, 52-Outlet hole, 60-First guide tube, 70-Second guide tube, 80-Flow hole, 90-Draining tube, 91-Connecting hole.

[0017] The realization of the purpose, functional features and advantages of this application will be further explained in conjunction with the embodiments and with reference to the accompanying drawings. Detailed Implementation

[0018] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0019] It should be noted that all directional indicators (such as up, down, left, right, front, back, etc.) in this utility model embodiment are only used to explain the relative positional relationship and movement of each component in a certain specific posture (as shown in the figure). If the specific posture changes, the directional indicator will also change accordingly.

[0020] In this utility model, unless otherwise explicitly specified and limited, the terms "connection," "fixing," etc., should be interpreted broadly. For example, "fixing" can mean a fixed connection, a detachable connection, or an integral part; it can mean a mechanical connection or an electrical connection; it can mean a direct connection or an indirect connection through an intermediate medium; it can mean the internal communication of two components or the interaction between two components, unless otherwise explicitly limited. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0021] Furthermore, if the embodiments of this utility model involve descriptions such as "first" or "second," these descriptions are for descriptive purposes only and should not be construed as indicating or implying their relative importance or implicitly specifying the number of technical features indicated. Therefore, a feature defined with "first" or "second" may explicitly or implicitly include at least one of those features. Additionally, the meaning of "and / or" throughout the text includes three parallel solutions; for example, "A and / or B" includes solution A, solution B, or a solution where both A and B are satisfied simultaneously. Furthermore, the technical solutions of the various embodiments can be combined with each other, but this must be based on the ability of those skilled in the art to implement them. When the combination of technical solutions is contradictory or impossible to implement, it should be considered that such a combination of technical solutions does not exist and is not within the scope of protection claimed by this utility model.

[0022] Example 1 Reference Figures 1-4 As shown, the first embodiment of this application provides a centrifuge cup, comprising: The cup body 10 has a receiving tank 11 for holding the cell fluid to be centrifuged; A cup lid 20 is disposed on the cup body 10 and is used to close the receiving groove 11. The cup lid 20 has a liquid inlet channel 21 and a liquid outlet channel 22 that are separated from each other. The liquid inlet channel 21 is connected to the receiving groove 11, and the first liquid outlet end of the liquid outlet channel 22 passes through the inner wall of the cup lid 20 near the receiving groove 11. The first outlet end of the outlet channel 22 is connected to a rotatable guide plate 30. The guide plate 30 has a guide channel 32 that communicates with the outlet channel 22. The guide plate 30 is provided with a guide hole 33 that communicates with the guide channel 32 and the receiving groove 11. The guide hole 33 is located on the periphery of the first outlet end of the outlet channel 22.

[0023] In this embodiment, the cup body 10 is cylindrical in shape with an open upper end. The cup lid 20 is detachably mounted on the upper end of the cup body 10, and the lid 20 and cup body 10 are threaded together. During centrifugation, a motor drives the cup body 10 and lid 20 to rotate as a whole, while the lid 20 and cup body 10 remain relatively stationary and rotate synchronously. A liquid inlet channel 21 is provided on the lid 20 to allow the cell fluid to be centrifuged to enter the containing tank 11 for centrifugation. A liquid outlet channel 22 is also provided on the lid 20. The separated solution first enters the liquid outlet channel 22 through its first outlet end and is then discharged. A guide plate 30 is provided at the first outlet end, such as... Figure 1 As shown, the guide plate 30 is positioned to the right of the rotation centerline of the cup lid 20. The guide plate 30 has guide holes 33 and a guide channel 32 inside, connecting the guide holes 33 and the outlet channel 22. This means that the fluid separated by centrifugation within the cup body 10 first enters the guide channel 32 through the guide holes 33 on the guide plate 30, then enters the outlet channel 22, and is finally discharged through the outlet channel 22. By rotating the guide plate 30, the distance between the guide holes 33 and the rotation centerline of the cup lid 20 can be varied. This allows users to adjust the outlet radius of solutions according to their actual needs, such as separating different types of cell fluids or exporting solutions with different radii, simply by rotating the guide plate 30, without needing to use centrifuge cups with different outlet channel lengths 22. Using only one centrifuge cup to achieve different centrifugation radii reduces operating costs compared to using multiple centrifuge cups. Furthermore, it allows for technical modifications based on existing centrifuge cups without requiring the addition or removal of other components, thus reducing the difficulty of the modifications.

[0024] Example 2 like Figure 2As shown, as an optional implementation, this embodiment provides a specific structure of a flow guide plate 30, including: a positioning groove 23 is provided on the inner wall of the cup lid 20, the flow guide plate 30 is disposed in the positioning groove 23, and a positioning sealing ring 34 is provided between the outer wall of the flow guide plate 30 and the inner wall of the positioning groove 23.

[0025] Specifically, a positioning groove 23 is provided on the upper inner wall of the cup lid 20, and the guide plate 30 is installed in the positioning groove 23, so that the lower end face of the guide plate 30 can be flush with the inner wall of the cup lid 20. A positioning sealing ring 34 is provided between the outer wall of the guide plate 30 along the circumference and the inner wall of the positioning groove 23 to ensure the sealing between the guide plate 30 and the positioning groove 23, ensuring that the solution can only enter the outlet channel 22 through the guide hole 33, and will not enter the outlet channel 22 through the gap between the guide plate 30 and the positioning groove 23. An annular first groove is provided on the outer wall of the guide plate 30 along the circumference, and an annular second groove is provided on the inner wall of the positioning groove 23. The first groove and the second groove are engaged to form a limiting space for accommodating the positioning sealing ring 34. The positioning sealing ring 34 is placed in the limiting space, which can also limit the guide plate 30. It should be noted that when it is necessary to rotate the guide plate 30 along its own axis, the cup lid 20 and the cup body 10 can be separated first, and then a circumferential external force can be applied to the guide plate 30 through the guide hole 33. That is, by setting the guide plate 30, it is equivalent to increasing the length of the liquid outlet channel 22, and the position of the solution entering the liquid outlet channel 22 can be adjusted through the guide hole 33.

[0026] Optionally, the cross-sectional area of ​​the flow channel 32 gradually decreases from the first liquid outlet end along the direction close to the flow hole 33.

[0027] Specifically, by gradually decreasing the cross-sectional area of ​​the flow channel 32 from left to right, the solution can be quickly transported to the outlet channel 22 after entering the flow channel 32 through the flow hole 33. It should be noted that... Figure 2 The guide plate 30 shown is only an example to illustrate its structural shape. The height of the guide plate 30, the length and height of the guide channel 32, etc. can be set according to actual usage requirements.

[0028] Optionally, the guide plate 30 has a connecting protrusion 31 for connecting to the first liquid outlet end, and the connecting protrusion 31 is interference-fitted with the first liquid outlet end.

[0029] Specifically, by providing a connecting flange at the upper end of the guide plate 30, and the connecting ring 31 being interference-fitted with the first liquid outlet end, the guide plate 30 is ensured to be fixed on the inner wall of the cup lid 20, and the guide plate 30 will not rotate arbitrarily. This ensures that the rotation of the guide plate 30 requires overcoming a certain external force, and that when the guide plate 30 is not subjected to circumferential external force, it will not move arbitrarily during normal centrifugal separation.

[0030] Example 3 like Figure 3 As an optional implementation, this embodiment provides a specific structure for a dynamic sealing seat 40, including: The dynamic sealing seat 40 has a flow hole 80 that communicates with the second liquid outlet end of the liquid outlet channel 22; A connector 50 is provided above the cup lid 20. The connector 50 has a liquid inlet hole 51 and a liquid outlet hole 52. The connector 50 is provided with a first guide tube 60 and a second guide tube 70. The second guide tube 70 is sleeved around the first guide tube 60, and the two ends of the first guide tube 60 protrude beyond the two ends of the second guide tube 70. The first guide tube 60 passes through the dynamic sealing seat 40 and connects the liquid inlet hole 51 and the liquid inlet channel 21; A flow passage is formed between the inner wall of the second guide tube 70 and the outer wall of the first guide tube 60, and the flow passage connects the liquid outlet 52 and the flow passage 80.

[0031] Specifically, the dynamic seal seat 40 is mounted on the cup lid 20. The dynamic seal seat 40 has a flow passage 80, and the second outlet end of the flow passage 80 and the liquid outlet channel 22 are interconnected. Figure 1As shown, the left end of the liquid outlet channel 22 is the second liquid outlet end, and the right end of the liquid outlet channel 22 is the first liquid outlet channel 22. During centrifugation, the dynamic seal seat 40, the cup lid 20, and the cup body 10 rotate as a single rotating unit. The connector 50 above the cup lid 20 does not rotate with the rotating unit. The connector 50 is provided with an inlet hole 51 and an outlet hole 52. The inlet hole 51 is located above the outlet hole 52. The inlet hole 51 and the outlet hole 52 can be connected to pipes for liquid transport. The upper end of the first guide tube 60 is connected to the inlet hole 51, and the lower end of the first guide tube 60 passes through the dynamic seal seat 40 and is connected to the inlet channel 21. That is, the cell fluid to be centrifuged enters the first guide tube through the inlet hole 51 on the connector 50, then enters the inlet channel 21, and then enters the container tank 11 for centrifugation. The second guide tube 70 is sleeved around the first guide tube 60, and the inner diameter of the second guide tube 70 is larger than the outer diameter of the first guide tube 60. The length of the second guide tube 70 is shorter than the length of the first guide tube 60. After the first guide tube 60 and the second guide tube 70 are installed, the first guide tube 60 is continuously connected to the second guide tube 70 from top to bottom. A flow channel is formed between the inner wall of the second guide tube 70 and the outer wall of the first guide tube 60. The upper end of the flow channel is connected to the liquid outlet 52, and the lower end of the flow channel is connected to the flow hole 80. That is, the solution separated in the receiving tank 11 first enters the guide channel 32 through the guide hole 33 on the guide plate 30, then enters the liquid outlet channel 22 through the guide channel 32, enters the flow channel through the flow hole 80, and finally is discharged through the liquid outlet 52.

[0032] Optionally, the cup lid 20 has a fixing groove for mounting the dynamic sealing seat 40, and a first sealing ring 41 is provided between the outer wall of the dynamic sealing seat 40 and the inner wall of the fixing groove, respectively located on the upper and lower sides of the flow hole 80.

[0033] Specifically, by setting the first sealing ring 41, it is ensured that the solution entering the flow hole 80 can only be discharged through the flow channel, and there will be no leakage through the gap between the dynamic sealing seat 40 and the cup lid 20.

[0034] Optionally, a bearing 44 is provided on the dynamic seal seat 40, a second guide tube 70 passes through the bearing 44, and a second sealing ring 42 is provided between the second guide tube 70 and the dynamic seal seat 40.

[0035] Specifically, by setting a bearing 44, the second guide tube 70 is supported without affecting the rotation of the dynamic sealing seat 40 relative to the second guide tube 70. By setting a second sealing ring 42, it is ensured that the solution in the flow hole 80 can only enter the second guide tube 70 for discharge, and will not leak through the gap between the second guide tube 70 and the dynamic sealing seat 40.

[0036] Optionally, a third sealing ring 43 is provided between the lower end of the first guide tube 60 protruding from the lower end of the second guide tube 70 and the dynamic sealing seat 40.

[0037] Specifically, by setting a third sealing ring 43, leakage of the solution in the flow hole 80 through the gap between the first guide pipe 60 and the dynamic sealing seat 40 is prevented.

[0038] Optionally, the first guide tube 60 passes through one end of the dynamic seal seat 40 and is connected to a dynamic seal support 45.

[0039] Specifically, by providing a dynamic sealing support 45, it is possible to prevent the integral formed by the connector 50, the first guide tube 60, and the second guide tube 70 from falling off the dynamic sealing seat 40.

[0040] Optionally, the bottom of the cup body 10 is provided with at least one radially extending drainage tube 90, the inner end of all drainage tubes 90 is connected to the liquid inlet channel 21, and the outer end of all drainage tubes 90 has a communication hole 91 that communicates with the receiving groove 11.

[0041] Specifically, by setting up drainage tubes 90, the cell fluid to be centrifuged, which enters the container 11 through the inlet channel 21, can be transported to the edge of the container 11. In this embodiment, three drainage tubes 90 are evenly arranged at the bottom of the cup body 10. When the cell fluid enters, it is divided into three independent drainage tubes 90, which can significantly reduce the flow rate and evenly distribute the cells in all directions of the centrifuge cup, thereby improving the cell recovery rate. A sleeve is provided in the middle of the three drainage tubes 90. The sleeve is used to cooperate with the inlet channel 21. That is, the cell fluid in the inlet channel 21 first enters the sleeve, and then enters each drainage tube 90 through the connection port between the sleeve and the drainage tube 90, and then enters the container through the connecting hole 91. It should be noted that a liquid inlet pipe is provided at the lower end of the cup lid 20, and the inside of the liquid inlet pipe is the liquid inlet channel 21. The liquid inlet pipe and the sleeve are overlapped, so as not to affect the disassembly or installation of the cup lid 20 and the cup body 10. The drainage tube 90 can be a complete pipe, or similarly, the drainage tube 90 can be as follows: Figure 4 The structure shown is that the drainage tube 90 is a long strip structure. A first drainage groove is set at the lower end of the drainage tube 90, and a second drainage groove is set at the bottom of the cup body 10. The drainage tube 90 is installed in the second drainage groove, and a drainage space is formed between the first drainage groove and the second drainage groove. A notch is set at the outer end of the drainage tube 90. The cell fluid in the liquid inlet channel 21 first enters the sleeve, and then enters each drainage space through the sleeve. Then it enters the receiving tank 11 through the notch for centrifugal separation.

[0042] The above are merely preferred embodiments of this application and do not limit the patent scope of this application. Any equivalent structural or procedural transformations made using the content of this application's specification and drawings, or direct or indirect applications in other related technical fields, are similarly included within the patent protection scope of this application.

Claims

1. A centrifuge cup, characterized in that, include: The cup body has a container for holding the cell fluid to be centrifuged; A cup lid is disposed on the cup body and used to close the receiving groove. The cup lid has a liquid inlet channel and a liquid outlet channel that are separated from each other. The liquid inlet channel is connected to the receiving groove, and the first liquid outlet end of the liquid outlet channel penetrates the inner wall of the cup lid near the receiving groove. The liquid outlet channel has a first liquid outlet end connected to a rotatable guide plate. The guide plate has a guide channel inside that communicates with the liquid outlet channel. The guide plate is provided with a guide hole that communicates with the guide channel and the receiving groove. The guide hole is located on the periphery of the first liquid outlet end of the liquid outlet channel.

2. The centrifuge cup as described in claim 1, characterized in that, The inner wall of the cup lid is provided with a positioning groove, the flow guide plate is disposed in the positioning groove, and a positioning sealing ring is provided between the outer wall of the flow guide plate and the inner wall of the positioning groove.

3. The centrifuge cup as described in claim 1, characterized in that, The cross-sectional area of ​​the flow channel gradually decreases from the first liquid outlet end along the direction close to the flow guide hole.

4. The centrifuge cup as described in claim 1, characterized in that, The guide plate has a connecting protrusion for connecting to the first liquid outlet end, and the connecting protrusion is interference-fitted with the first liquid outlet end.

5. The centrifuge cup as described in claim 1, characterized in that, The cup lid is also provided with a dynamic sealing seat, which has a flow hole communicating with the second liquid outlet end of the liquid outlet channel; A connector is provided above the cup lid, and the connector has a liquid inlet and a liquid outlet. The connector is provided with a first guide tube and a second guide tube. The second guide tube is sleeved around the first guide tube, and the two ends of the first guide tube protrude beyond the two ends of the second guide tube. The first guide tube passes through the dynamic sealing seat and connects the liquid inlet hole and the liquid inlet channel; A flow passage is formed between the inner wall of the second guide tube and the outer wall of the first guide tube, and the flow passage connects the liquid outlet and the flow passage.

6. The centrifuge cup as described in claim 5, characterized in that, The cup lid has a fixing groove for installing the dynamic sealing seat, and a first sealing ring is provided between the outer wall of the dynamic sealing seat and the inner wall of the fixing groove, respectively located on the upper and lower sides of the flow hole.

7. The centrifuge cup as described in claim 5, characterized in that, A bearing is provided on the dynamic seal seat, the second guide tube passes through the bearing, and a second sealing ring is provided between the second guide tube and the dynamic seal seat.

8. The centrifuge cup as described in claim 5, characterized in that, A third sealing ring is provided between the lower end of the first guide tube, which protrudes beyond the lower end of the second guide tube, and the dynamic sealing seat.

9. The centrifuge cup as described in claim 5, characterized in that, The first guide tube passes through one end of the dynamic sealing seat and is connected to a dynamic sealing support.

10. The centrifuge cup as described in claim 1, characterized in that, The bottom of the cup body is provided with at least one radially extending drainage tube, the inner end of all the drainage tubes is connected to the liquid inlet channel, and the outer end of all the drainage tubes has a connecting hole that communicates with the receiving groove.

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