Fully automated nucleic acid extractor

Abstract

The utility model relates to the field of laboratory equipment, specifically disclose full -automatic nucleic acid extractor, including drive assembly and extraction component, and extraction component includes sample holder, first fixed mount, second fixed mount, magnet and stick cover, a plurality of magnet is arranged on first fixed mount, a plurality of stick cover is arranged on second fixed mount, and sample holder is set below stick cover, and stick cover is set below corresponding magnet, drive assembly includes moving seat and sets up first execution element, second execution element, third execution element, limit lead screw on moving seat, and first execution element drives first fixed mount to go up and down through first sliding block, and second execution element drives second fixed mount to go up and down through second sliding block, and first sliding block and second sliding block are lifted along limit lead screw respectively, and third execution element drives moving seat to carry out limit movement, the utility model whole size reduces, and space utilization greatly improves, and application scene is more extensive, and operation is more stable and reliable.

Description

Technical Field

[0001] This utility model relates to the field of laboratory equipment, and in particular to a nucleic acid purification instrument. Background Technology

[0002] Currently, nucleic acid purification instruments, which utilize magnetic bead technology for fully automated nucleic acid extraction, are widely used in fields such as genomics, disease control and medical treatment, food safety, and forensic medicine. This instrument achieves automated DNA / RNA separation through magnetic bead adsorption and transfer, supporting multiple sample types including blood, animal and plant tissues.

[0003] Currently available 32-channel fully automated nucleic acid extraction and purification instruments are typically large, taking up considerable laboratory space; this can be inconvenient for labs with limited space. Furthermore, because the magnetic rods need to pick up and transfer magnetic beads from the sample, the movement path of the magnetic rods requires precise control.

[0004] The technical problem to be solved in this application is: how to make the structure of a 32-channel fully automated nucleic acid extraction and purification instrument more compact. Utility Model Content

[0005] In order to overcome the shortcomings of the existing technology, the purpose of this utility model is to provide a fully automated nucleic acid extractor.

[0006] The technical solution adopted by this utility model is as follows: a fully automatic nucleic acid extractor, including a driving component and an extraction component. The extraction component includes a sample holder, a first fixed frame, a second fixed frame, magnetic rods, and rod sleeves. Several magnetic rods are arranged on the first fixed frame, and several rod sleeves are arranged on the second fixed frame. The sample holder is located below the rod sleeves, and the rod sleeves are located below the corresponding magnetic rods. The driving component includes a moving base and a first actuator, a second actuator, a third actuator, and a limiting screw arranged on the moving base. The first actuator drives the first fixed frame to rise and fall through a first slider, and the second actuator drives the second fixed frame to rise and fall through a second slider. The first slider and the second slider rise and fall along the limiting screw, respectively. The third actuator drives the moving base to move along the Y-axis.

[0007] In some embodiments, the second fixed frame is provided with a replacement groove, and the rod sleeves in the same row are fixedly installed on the replacement platform. The replacement platform slides at the upper limit of the replacement groove, and the upper end of the replacement groove is connected to the outside.

[0008] In some embodiments, the fixed end of the first actuator is fixedly connected to the movable seat, the output end drives the limit screw to rotate at the upper limit of the movable seat, and the first slider is threadedly engaged with the limit screw.

[0009] In some embodiments, the second actuator is provided with a Z-axis driving wheel, a Z-axis driven wheel, and a Z-axis synchronous belt. The Z-axis driving wheel and the Z-axis driven wheel are respectively driven and cooperate with the Z-axis synchronous belt. The Z-axis driving wheel and the Z-axis driven wheel rotate at the upper limit of the moving seat. The fixed end of the second actuator is fixedly connected to the moving seat, and the output end drives the Z-axis driving wheel. The second slider is fixedly connected to the Z-axis synchronous belt, and the second slider is movably sleeved on the limit screw.

[0010] In some implementations, the movable seat is provided with a limiting block, which is located below the Z-axis drive wheel.

[0011] In some embodiments, the fully automated nucleic acid extractor includes an outer frame and a base plate, which are fixedly connected to form a workspace. The workspace is divided into three areas, including a working area and a driving area. The driving component is located in the driving area, and the extraction component is located in the working area. The working area and the driving area are isolated from each other by a first isolation plate.

[0012] In some embodiments, the first isolation plate is provided with a movable hole, through which the working area and the driving area are connected, and the first slider and the second slider move within the movable hole.

[0013] In some embodiments, the movable seat is provided with a baffle plate, the baffle plate has a movable hole that communicates with the movable hole, the baffle plate is fixedly connected to the movable seat, and the width of the movable hole is just enough to allow the first slider and the second slider to pass through.

[0014] In some embodiments, an ultraviolet lamp is provided above the extraction component, and the ultraviolet lamp is fixedly connected to the first isolation plate.

[0015] In some implementations, a heating element is fixedly provided at the bottom of the base plate, and the heating element is correspondingly positioned below the sample holder.

[0016] The beneficial effects of this utility model are as follows:

[0017] This fully automated nucleic acid extractor reduces the lateral width of the equipment by setting up the magnetic rod, rod sleeve and sample holder in a vertical direction. The drive components are set on the same moving seat, reducing the number of parts. The first slider and the second slider move along the same limiting screw, which can ensure the vertical alignment between the magnetic rod and the rod sleeve. The structure is simple and stable and can work for a long time. It is convenient for users to replace the rod sleeve. The overall size of the machine is reduced, the space utilization rate is greatly improved, the production cost is correspondingly lower, the application scenarios are wider, and the operation is more stable and reliable. Attached Figure Description

[0018] Figure 1 This is a schematic diagram of the structure of an embodiment;

[0019] Figure 2 This is a schematic diagram of the internal structure (power supply hidden) of an embodiment;

[0020] Figure 3 This is a side view of the internal structure (power supply hidden) of an embodiment.

[0021] Figure 4 This is a top view of the internal structure (power supply hidden) of the embodiment.

[0022] The labels and names in the diagram correspond as follows: 1. Outer frame; 2. Base plate; 3. Running component; 4. Drive component; 5. Extraction component; 11. Working area; 12. Drive area; 13. Running area; 14. First isolation plate; 15. Second isolation plate; 16. Ultraviolet lamp; 17. Heating component; 40. Moving seat; 41. First actuator; 42. Second actuator; 43. Third actuator; 44. First slider; 45. Second slider; 46. Limiting screw; 51. Sample holder; 52. First fixing frame; 53. Second fixing frame; 54. Magnetic rod; 55. Rod sleeve; 56. Changing slot; 57. Changing table; 421. Z-axis driving wheel; 422. Z-axis driven wheel; 423. Z-axis synchronous belt; 424. Limiting block; 431. Y-axis driving wheel; 432. Y-axis driven wheel; 433. Y-axis synchronous belt; 434. Fixed guide rail. Detailed Implementation

[0023] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0024] Please see Figure 1 This utility model provides a technical solution: a fully automated nucleic acid extractor, including an outer frame 1, a base plate 2, a running component 3, a driving component 4, and an extraction component 5. The outer frame 1 and the base plate 2 are fixedly connected and form a working space, and the running component 3, the driving component 4, and the extraction component 5 are arranged within the working space formed by the outer frame 1 and the base plate 2.

[0025] Please see Figure 4 The workspace is divided into three areas: work area 11, drive area 12 and running area 13. Extraction component 5 is set in work area 11, drive component 4 is set in drive area 12, and running component 3 is set in running area 13. Work area 11 and drive area 12 are isolated from each other by a first isolation plate 14, and drive area 12 and running area 13 are separated from each other by a second isolation plate 15.

[0026] Please see Figure 2-4The extraction component 5 includes a sample holder 51, a first fixing frame 52, a second fixing frame 53, magnetic rods 54, and rod sleeves 55. Several magnetic rods 54 are arranged in an orderly fashion on the first fixing frame 52, with one rod sleeve corresponding to one magnetic rod 54. Corresponding magnetic rods 54 and rod sleeves 55 are aligned on the same straight line, with the rod sleeves 55 positioned below the corresponding magnetic rods 54. Several rod sleeves 55 are arranged in an orderly fashion on the second fixing frame 53, with the sample holder 51 positioned below the rod sleeves 55. To facilitate the replacement of the rod sleeves 55, the second fixing frame 53 has a replacement groove 56. Rod sleeves 55 in the same row are fixedly mounted on a replacement platform 57. The replacement platform 57 slides within the upper limit of the replacement groove 56, with one end protruding from the replacement groove 56 to facilitate manual removal or insertion. The upper end of the replacement groove 56 communicates with the outside, allowing the magnetic rods 54 to enter the rod sleeves 55. The bottom of the rod sleeves 55 is closed to isolate the magnetic rods 54 from the sample liquid. The magnetic rod 54 draws in the magnetic beads in the sample through the rod sleeve 55.

[0027] Please see Figure 3 and Figure 4 The drive assembly 4 includes a movable base 40, a first actuator 41, a second actuator 42, a third actuator 43, a first slider 44, a second slider 45, and a limiting screw 46. The first actuator 41, the second actuator 42, and the third actuator 43 are all motors with rotating output ends. The fixed end of the first actuator 41 is fixedly connected to the movable base 40, and its output end drives the limiting screw 46 to rotate at the upper limit on the movable base 40. The first slider 44 is threadedly engaged with the limiting screw 46, so when the limiting screw 46 rotates, the first slider 44 moves up and down. The first slider 44 is fixedly connected to the first fixed frame 52. The second actuator 42 is equipped with a Z-axis drive wheel 421, a Z-axis driven wheel 422, and a Z-axis synchronous belt 423. The Z-axis drive wheel 421 and the Z-axis driven wheel 422 are respectively driven by the Z-axis synchronous belt 423. The Z-axis drive wheel 421 and the Z-axis driven wheel 422 rotate at their upper limits on the movable seat 40. The fixed end of the second actuator 42 is fixedly connected to the movable seat 40, and the output end drives the Z-axis drive wheel 421. The second slider 45 is fixedly connected to the Z-axis synchronous belt 423. Therefore, when the Z-axis synchronous belt 423 rotates, the second slider 45 moves. The second slider 45 is movably sleeved on the limiting screw 46 and moves along the limiting screw 46. Therefore, the moving directions of the first slider 44 and the second slider 45 are the same. The second slider 45 is fixedly connected to the second fixed frame 53. Since the first slider 44 and the second slider 45 move up and down along the limiting screw 46 respectively, the accuracy of the axial movement of the magnetic rod 54 and the sleeve 55 can be improved, ensuring the accuracy of the movement path of the magnetic rod 54.

[0028] In order to limit the upward stroke of the second slider 45, the moving seat 40 is provided with a limiting block 424. The limiting block 424 is located below the Z-axis drive wheel 421, and the limiting block 424 restricts the second slider 45 from moving in the direction of the drive wheel.

[0029] The third actuator 43 is equipped with a Y-axis drive wheel 431, a Y-axis driven wheel 432, and a Y-axis synchronous belt 433. The Y-axis drive wheel 431 and the Y-axis driven wheel 432 are respectively driven by the Y-axis synchronous belt 433. The Y-axis drive wheel 431 and the Y-axis driven wheel 432 are respectively limited to rotating at the upper limit on the base plate 2. The fixed end of the third actuator 43 is fixedly connected to the base plate 2, and the output end drives the Y-axis drive wheel 431. The movable seat 40 is fixedly connected to the Y-axis synchronous belt 433. In order to further limit the movement path of the movable seat 40, the base plate 2 is provided with a fixed guide rail 434, and the movable seat 40 moves along the fixed guide rail 434 for limited movement.

[0030] When the third actuator 43 operates, the movable seat 40 moves, and the first slider 44 and the second slider 45 drive the first fixed frame 52 and the second fixed frame 53. To allow sufficient space for movement, the first isolation plate 14 is provided with a moving hole, through which the working area 11 and the driving area 12 are connected. The first slider 44 and the second slider 45 move within the moving hole. To further prevent sample liquid in the working area 11 from contaminating the driving area 12, the movable seat 40 is provided with a baffle plate. The baffle plate is provided with a moving hole, which is connected to the movable hole. The width of the moving hole is just enough to allow the first slider 44 and the second slider 45 to pass through. The baffle plate is fixedly connected to the movable seat 40, further blocking the connection between the driving area 12 and the working area 11, reducing the communication between the driving area 12 and the working area 11.

[0031] The operating area 13 houses components related to operation, such as cooling fans, drivers, and filters. Due to the large size of the power supply, it is positioned across the driver area 12 and the operating area 13.

[0032] To facilitate disinfection of the work area 11, in this embodiment, preferably, an ultraviolet lamp 16 is provided above the extraction component 5, and the ultraviolet lamp 16 is fixedly connected to the first isolation plate 14.

[0033] In order to heat the sample, in this embodiment, preferably, a heating component 17 is fixedly provided at the bottom of the base plate 2. The heating component 17 is correspondingly arranged below the sample holder 51. The heating component 17 can be a part used for heating, such as a heating plate, heating wire, etc.

[0034] The working principle and usage process of this utility model are as follows: The second actuator 42 drives the rod sleeve 55 to descend into the sample. Through repeated lifting and lowering, the sample liquid is fully oscillated. After oscillation, the first actuator 41 drives the magnetic rod 54 to descend. The magnetic rod 54 picks up the magnetic beads in the sample liquid through the rod sleeve 55. The first actuator 41 and the second actuator 42 drive the magnetic rod 54 and the rod sleeve 55 to rise together. The third actuator 43 drives the moving seat 40 to move. The magnetic rod 54 and the rod sleeve 55 are transferred to the top of another sample. The magnetic rod 54 rises and the rod sleeve 55 falls, thereby completing the transfer of the magnetic beads.

[0035] Finally, it should be noted that the above description is merely a preferred embodiment of this utility model and is not intended to limit the utility model. Although the utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this utility model should be included within the protection scope of this utility model.

Claims

1. A fully automated nucleic acid extractor, comprising a drive assembly (4) and an extraction assembly (5), characterized in that, The extraction component (5) includes a sample holder (51), a first fixing frame (52), a second fixing frame (53), magnetic rods (54), and rod sleeves (55). A plurality of magnetic rods (54) are arranged on the first fixing frame (52), and a plurality of rod sleeves (55) are arranged on the second fixing frame (53). The sample holder (51) is positioned below the rod sleeves (55), and the rod sleeves (55) are positioned below the corresponding magnetic rods (54). The driving component (4) includes a movable base (40) and a mechanism positioned on the movable base (40). The device includes a first actuator (41), a second actuator (42), a third actuator (43), and a limiting screw (46). The first actuator (41) drives the first fixed frame (52) to rise and fall through the first slider (44). The second actuator (42) drives the second fixed frame (53) to rise and fall through the second slider (45). The first slider (44) and the second slider (45) rise and fall along the limiting screw (46) respectively. The third actuator (43) drives the moving seat (40) to perform a limiting movement.

2. The fully automated nucleic acid extractor according to claim 1, characterized in that, The second fixing frame (53) is provided with a replacement groove (56), and the rod sleeves (55) in the same column are fixedly installed on the replacement table (57). The replacement table (57) slides at the upper limit of the replacement groove (56), and the upper end of the replacement groove (56) is connected to the outside.

3. The fully automated nucleic acid extractor according to claim 1, characterized in that, The fixed end of the first actuator (41) is fixedly connected to the moving seat (40), and the output end drives the limit screw (46) to rotate at the upper limit on the moving seat (40). The first slider (44) is threadedly engaged with the limit screw (46).

4. The fully automated nucleic acid extractor according to claim 1, characterized in that, The second actuator (42) is provided with a Z-axis drive wheel (421), a Z-axis driven wheel (422) and a Z-axis synchronous belt (423). The Z-axis drive wheel (421) and the Z-axis driven wheel (422) are respectively driven and cooperate with the Z-axis synchronous belt (423). The Z-axis drive wheel (421) and the Z-axis driven wheel (422) rotate at the upper limit on the moving seat (40). The fixed end of the second actuator (42) is fixedly connected to the moving seat (40), and the output end drives the Z-axis drive wheel (421). The second slider (45) is fixedly connected to the Z-axis synchronous belt (423), and the second slider (45) is movably sleeved on the limit screw (46).

5. The fully automated nucleic acid extractor according to claim 4, characterized in that, The movable seat (40) is provided with a limiting block (424), which is located below the Z-axis drive wheel (421).

6. The fully automated nucleic acid extractor according to claim 1, characterized in that, The fully automated nucleic acid extractor includes an outer frame (1) and a base plate (2). The outer frame (1) and the base plate (2) are fixedly connected to form a workspace. The workspace is divided into three areas, including a working area (11) and a driving area (12). The driving component (4) is located in the driving area (12), and the extraction component (5) is located in the working area (11). The working area (11) and the driving area (12) are isolated from each other by a first isolation plate (14).

7. The fully automated nucleic acid extractor according to claim 6, characterized in that, The first isolation plate (14) is provided with a moving hole, and the working area (11) and the driving area (12) are connected through the moving hole. The first slider (44) and the second slider (45) move within the moving hole.

8. The fully automated nucleic acid extractor according to claim 6, characterized in that, The movable seat (40) is provided with a baffle plate, the baffle plate is provided with a movable hole, the movable hole is connected to the movable hole, the baffle plate is fixedly connected to the movable seat (40), and the width of the movable hole is just enough to allow the first slider (44) and the second slider (45) to pass through.

9. The fully automated nucleic acid extractor according to claim 6, characterized in that, An ultraviolet lamp (16) is provided above the extraction component (5), and the ultraviolet lamp (16) is fixedly connected to the first isolation plate (14).

10. The fully automated nucleic acid extractor according to claim 6, characterized in that, The bottom of the base plate (2) is fixedly provided with a heating element, which is correspondingly positioned below the sample holder (51).

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