Mesenchymal stem cell serum-free culture medium preparation equipment

Abstract

The utility model relates to culture medium preparation technical field, concretely disclose a kind of mesenchymal stem cell serum-free medium preparation equipment, including the sealing cover of being arranged in beaker mouth, and sealing cover is according to function and is divided into the inner cover and outer cover of mutual rotation connection, rotation is provided with rotating ball on outer cover, rotating ball is opened and is inserted with the perforation of medicine spoon, rotating ball and outer cover between being provided with tension spring.The mesenchymal stem cell serum-free medium preparation equipment provided by the utility model, by rotating ball being located in the conical tip position of beaker, the medicine spoon above rotating ball just keeps maximum skew angle, it can make the medicine spoon head below cover beaker bottom space, further to the medicine spoon below rotating ball segment form the conical stirring range of same slope with beaker, simultaneously tension spring will pull rotating ball to tilt predetermined angle, so as to make medicine spoon keep at maximum inclination, further guarantee the stirring capacity of medicine spoon, improve the mixing efficiency to raw material.

Description

Technical Field

[0001] This utility model relates to the field of culture medium preparation technology, and more specifically to a device for preparing serum-free culture medium for mesenchymal stem cells. Background Technology

[0002] Human mesenchymal stem cells were first isolated from bone marrow. They are tissue stem cells derived from the mesoderm with self-renewal capabilities and can form other human cells in specific in vivo and in vitro environments. Before culturing stem cells, serum-free culture medium needs to be prepared to meet the requirements of a suitable environment for stem cell culture.

[0003] According to the publication (announcement) number: CN111334466A, the publication (announcement) date: 2020-06-26, a mesenchymal stem cell sphere, its preparation method and application, and the application of the sphere-forming culture medium in the preparation of mesenchymal stem cell spheres are disclosed, including the sphere-forming culture medium for culturing mesenchymal stem cells.

[0004] In the prior art, including the aforementioned patent, the culture medium requires placing the raw materials and nutrients in a triangular beaker and adding appropriate distilled water before preparation. The bottom of the beaker is heated while stirring the distilled water and raw materials. However, this requires workers to continuously stir the raw materials with a spatula and check whether the powder floating on the liquid has completely dispersed until the powdery raw materials are completely dispersed in the distilled water. Due to the special shape of the bottom of the triangular beaker and the need for slow and stable stirring with a spatula, the time is relatively long. It is not possible to completely ensure that the spatula moves in a conical trajectory within the beaker. At the same time, because the nozzle of the beaker is small, there are certain safety issues when using electric stirring equipment, which may even damage the beaker under heating conditions. This prolongs the mixing time of the raw materials in the distilled water and also causes a large amount of steam generated during heating to be consumed, resulting in an increase in the concentration of the culture medium after preparation. Utility Model Content

[0005] The purpose of this invention is to provide a serum-free culture medium preparation device for mesenchymal stem cells, aiming to solve the problems mentioned above.

[0006] To achieve the above objectives, this utility model provides the following technical solution:

[0007] A serum-free culture medium preparation device for mesenchymal stem cells includes a beaker and a spoon placed inside it, and a sealing cap set at the mouth of the beaker. The sealing cap is divided into an inner cap and an outer cap that are rotatably connected to each other according to their functions.

[0008] The outer cover is rotatably provided with a rotating ball, and the rotating ball has a through hole for inserting into the medicine spoon;

[0009] A tension spring is provided between the rotating ball and the outer cover, and the tension spring in the default state makes the perforation horizontal.

[0010] Preferably, the outer cover is provided with a cover protrusion to accommodate the movement of the rotating ball.

[0011] Preferably, the cover protrusion has an annular groove located outside the rotating ball, and a slider that slides in the annular groove is fixedly installed on the tension spring.

[0012] Preferably, the inner cover has an inner opening, and the outer cover has an outer opening that communicates and cooperates with the inner opening.

[0013] Preferably, a sealing ring that fits into the mouth of the beaker is fixedly installed on the outer wall of the inner cover.

[0014] Preferably, the outer wall of the sealing ring is provided with an inclined section, and the inner wall of the outer cover is fixedly installed with an inclined block that slides in cooperation with the inclined section.

[0015] Preferably, the inclined section has an arc segment at its high position, and when the inclined block slides onto the arc segment, the inner and outer openings are staggered.

[0016] Preferably, the end of the slider is provided with an arc-shaped bulge.

[0017] Preferably, the sealing ring is a rubber ring.

[0018] Preferably, the rotating ball has a groove on its side wall.

[0019] In the above technical solution, the mesenchymal stem cell serum-free culture medium preparation device provided by this utility model has the following beneficial effects: by positioning the rotating ball at the cone tip of the triangular beaker, the purpose is that as long as the medicine spoon above the rotating ball maintains the maximum tilt angle, the head of the medicine spoon below can cover the bottom space of the beaker, thereby forming a conical stirring range with the same slope as the beaker on the part of the medicine spoon below the rotating ball. At the same time, the tension spring will pull the rotating ball to tilt at a predetermined angle so that the medicine spoon is kept at the maximum tilt angle, thereby ensuring the stirring ability of the medicine spoon, improving the mixing efficiency of raw materials, and reducing the stirring time. Attached Figure Description

[0020] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in this utility model. For those skilled in the art, other drawings can be obtained based on these drawings.

[0021] Figure 1 A schematic diagram of the beaker assembly and sealing cap provided in an embodiment of this utility model;

[0022] Figure 2This is a front sectional view of the beaker assembly sealing cap provided in an embodiment of the present utility model;

[0023] Figure 3 An exploded view of the inner cover, outer cover, and rotating ball provided for an embodiment of this utility model;

[0024] Figure 4 A schematic diagram of the inner cover, outer cover, and rotating ball assembly from a bottom view, provided for an embodiment of this utility model;

[0025] Figure 5 for Figure 4 Enlarged diagram of point A.

[0026] Explanation of reference numerals in the attached figures:

[0027] 1. Beaker; 2. Spoon; 3. Inner lid; 31. Inner opening; 32. Sealing ring; 33. Inclined section; 34. Arc section; 4. Outer lid; 41. Outer opening; 42. Lid protrusion; 43. Annular groove; 44. Inclined block; 5. Rotating ball; 51. Perforation; 52. Sliding block. Detailed Implementation

[0028] To enable those skilled in the art to better understand the technical solution of this utility model, the present utility model will be further described in detail below with reference to the accompanying drawings.

[0029] like Figure 1-5 As shown, a serum-free culture medium preparation device for mesenchymal stem cells includes a beaker 1 and a medicine spoon 2 placed inside it, and also includes a sealing cap set at the mouth of the beaker 1, and the sealing cap is divided into an inner cap 3 and an outer cap 4 that are rotatably connected to each other according to their functions.

[0030] A rotating ball 5 is rotatably mounted on the outer cover 4, and a through hole 51 is provided on the rotating ball 5 for insertion into the medicine spoon 2;

[0031] A tension spring is provided between the rotating ball 5 and the outer cover 4, and the tension spring in the default state makes the perforation 51 horizontal.

[0032] Specifically, the inner cover 3 and the outer cover 4 both face downwards, and both have holes that restrict the movement of the rotating ball 5, allowing the rotating ball 5 to move at multiple angles within the holes. An arc-shaped groove is provided on the side wall of the rotating ball 5, and one end of the tension spring is welded to the inner wall of the groove so that the tension spring can be reset to pull the rotating ball 5.

[0033] Furthermore, when the perforation 51 is vertical, the tension spring maintains maximum deformation. Therefore, the tension spring in the default state will inevitably pull the rotating ball 5 to tilt and reset direction. After the spoon 2 is inserted into the perforation 51, the upper end of the spoon 2 is the position where the spoon 2 is resisted, which is the position where the spoon 2 tilts to its maximum. At this time, the lower part of the spoon 2 is parallel to the side wall of the beaker 1.

[0034] After the raw materials are mixed, open the sealing cap and remove the spoon 2 from the outer cap 4, then replace the sealing cap. This allows the sealing cap to directly seal the mouth of the beaker 1, eliminating the need for a sealing film and elastic cord to seal the beaker 1, thus reducing operation time. The rotating ball 5 is located at the cone tip of the triangular beaker 1. The purpose is that the spoon 2 above the rotating ball 5 can maintain its maximum tilt angle so that the head of the spoon 2 below covers the bottom space of the beaker 1, thereby giving the spoon 2 a better stirring effect. At the same time, the tension spring will pull the rotating ball 5 to tilt at a predetermined angle so that the spoon 2 is kept at its maximum tilt angle, thus ensuring the stirring ability of the spoon 2, improving the mixing efficiency of the raw materials, and reducing the stirring time.

[0035] As a further embodiment of this utility model, the outer cover 4 is provided with a cover protrusion 42 for accommodating the movement of the rotating ball 5.

[0036] Specifically, the center of the rotating ball 5 is located inside the lid protrusion 42, and the rotating ball 5 slides within the lid protrusion 42, with the sliding gap meeting the sealing requirements of the beaker 1.

[0037] The cover protrusion 42 provides space for the rotating ball 5 to move, making the rotating ball 5 more stable when it moves. Under the action of the tension spring to restore the deformation, the rotating ball 5 can be pulled back to its original position and the perforation 51 can be horizontally placed into the cover protrusion 42, thereby realizing the leakage prevention and sealing function of the perforation 51.

[0038] As another embodiment further provided by this utility model, an annular groove 43 located outside the rotating ball 5 is provided in the cover protrusion 42, and a slider 52 located in the annular groove 43 is fixedly installed on the tension spring.

[0039] Specifically, the slider 52 is welded to the end of the tension spring, and the lower inner wall of the annular groove 43 is lower than the center of the rotating ball 5.

[0040] The slider 52 slides along the designated annular path so that the rotating ball 5 is not disturbed by the tension spring when it moves in the circumferential direction. At the same time, the annular groove 43 can also provide a space to accommodate the thickness of the tension spring when it fully recovers its deformation, ensuring that the perforation 51 is horizontally embedded in the cover protrusion 42 for sealing.

[0041] As another embodiment of this utility model, the inner cover 3 has an inner opening 31, and the outer cover 4 has an outer opening 41 that communicates and cooperates with the inner opening 31.

[0042] By rotating the outer cover 4 onto the inner cover 3, the obstruction range of the outer opening 41 on the inner opening 31 can be adjusted, thereby utilizing the channel left between the two to discharge the steam in the beaker 1, avoiding excessive pressure in the beaker 1. The adjustable channel can also be adjusted according to the heating time to meet the requirement of not discharging too much steam.

[0043] As another embodiment of this utility model, a sealing ring 32 that fits into the mouth of the beaker 1 is fixedly installed on the outer wall of the inner cover 3.

[0044] The opening on the lower end face of the sealing ring 32 is used to fit and clamp the mouth of the beaker 1, thereby meeting the requirement of sealing the mouth of the beaker 1, so that the inner cover 3 can ensure the sealing effect in the default state.

[0045] As a further embodiment of this utility model, the outer wall of the sealing ring 32 is provided with an inclined section 33, and the inner wall of the outer cover 4 is fixedly installed with an inclined block 44 that slides with the inclined section 33.

[0046] Specifically, the inclined segment 33 is an arc-shaped triangle with a gradually increasing radial range on the outer ring of the sealing ring 32, and the inclined block 44 matches the arc surface of the arc-shaped triangle.

[0047] During the process of adjusting the channel size by rotating the outer cover 4, the inclined block 44 actively slides to a radially increased position on the inclined section 33, thereby causing the inclined section 33 to be compressed towards the inner cover 3 axis, increasing the pressure effect of the sealing ring 32 on the mouth of the cup and strengthening the sealing ability of the mouth of the beaker 1.

[0048] As another embodiment provided by this utility model, the inclined segment 33 is provided with an arc segment 34 at a high position, and when the inclined block 44 slides onto the arc segment 34, the inner opening 31 and the outer opening 41 are misaligned.

[0049] Specifically, the maximum position of the arc segment 34 in the radial direction of the inclined segment 33 is no longer raised, so that the position of the sealing ring 32 on the arc segment 34 is kept under maximum pressure.

[0050] When the sealing cap is used to seal beaker 1, the channel is closed by the rotating outer cap 4, and the sealing ring 32 is compressed to its maximum sealing capacity. At this time, the sealing cap has the best sealing effect.

[0051] As another embodiment provided in this utility model, the end of the slider 52 is provided with an arc-shaped bulge.

[0052] The arc-shaped bulge that matches the low position of the annular groove 43 allows the slider 52 to slide in the annular groove 43 without disengaging, thus ensuring the stability of the rotating ball 5 during its movement.

[0053] As another embodiment further provided in this utility model, the sealing ring 32 is specifically a rubber ring.

[0054] The rubber ring material enables the sealing ring 32 to have basic sealing ability, and when combined with radial pressure, it enables the sealing cap to have a better sealing effect.

[0055] As another embodiment of this utility model, a groove is provided on the side wall of the rotating ball 5.

[0056] The groove is exposed upwards after the rotating ball 5 is reset, making it easier for the staff to press the groove to drive the tension spring to deform, thereby exposing the perforation 51 upwards.

[0057] Working principle: After the raw materials are stirred, the sealing cap is opened and the spoon 2 is removed from the outer cap 4. Then the sealing cap is put back on. Under the restoring deformation of the tension spring, the rotating ball 5 is pulled back to its original position, and the perforation 51 is horizontally placed into the protrusion 42 of the cap. This achieves the leak-proof sealing function of the perforation 51, which makes it easy to directly seal the mouth of the beaker 1 with the sealing cap. This eliminates the need to use a sealing film with an elastic rope to seal the beaker 1, reducing operation time. The rotating ball 5 is located at the cone tip of the triangular beaker 1. The purpose is that the spoon 2 above the rotating ball 5 can cover the bottom space of the beaker 1 by maintaining the maximum tilt angle, so that the head of the spoon 2 below can cover the bottom space of the beaker 1, thus giving the spoon 2 a better stirring effect.

[0058] The foregoing description only illustrates certain exemplary embodiments of the present invention. Undoubtedly, those skilled in the art can modify the described embodiments in various ways without departing from the spirit and scope of the present invention. Therefore, the above drawings and descriptions are illustrative in nature and should not be construed as limiting the scope of protection of the claims of the present invention.

Claims

1. A device for preparing serum-free culture medium for mesenchymal stem cells, comprising a beaker (1) and a spoon (2) placed inside it, characterized in that, It also includes a sealing cap set at the mouth of the beaker (1), and the sealing cap is divided into an inner cap (3) and an outer cap (4) that are rotatably connected to each other according to their functions. The outer cover (4) is rotatably provided with a ball (5), and the ball (5) has a through hole (51) for inserting into the medicine spoon (2). A tension spring is provided between the rotating ball (5) and the outer cover (4), and the tension spring in the default state makes the perforation (51) horizontal.

2. The equipment for preparing serum-free culture medium for mesenchymal stem cells according to claim 1, characterized in that, The outer cover (4) is provided with a cover protrusion (42) to accommodate the movement of the rotating ball (5).

3. The equipment for preparing serum-free culture medium for mesenchymal stem cells according to claim 2, characterized in that, The cover protrusion (42) has an annular groove (43) located outside the rotating ball (5), and a slider (52) is fixedly installed on the tension spring and slides in the annular groove (43).

4. The equipment for preparing serum-free culture medium for mesenchymal stem cells according to claim 2, characterized in that, The inner cover (3) has an inner opening (31), and the outer cover (4) has an outer opening (41) that communicates and cooperates with the inner opening (31).

5. The equipment for preparing serum-free culture medium for mesenchymal stem cells according to claim 4, characterized in that, The inner cover (3) is fixedly installed with a sealing ring (32) that fits into the mouth of the beaker (1) on the outer side wall.

6. The apparatus for preparing serum-free culture medium for mesenchymal stem cells according to claim 5, characterized in that, The outer wall of the sealing ring (32) is provided with an inclined section (33), and the inner wall of the outer cover (4) is fixedly installed with an inclined block (44) that slides with the inclined section (33).

7. The apparatus for preparing serum-free culture medium for mesenchymal stem cells according to claim 6, characterized in that, The inclined segment (33) is provided with an arc segment (34) at a high position, and when the inclined block (44) slides onto the arc segment (34), the inner opening (31) and the outer opening (41) are staggered.

8. The equipment for preparing serum-free culture medium for mesenchymal stem cells according to claim 3, characterized in that, The slider (52) has an arc-shaped protrusion at its end.

9. The apparatus for preparing serum-free culture medium for mesenchymal stem cells according to claim 6, characterized in that, The sealing ring (32) is specifically a rubber ring.

10. The apparatus for preparing serum-free culture medium for mesenchymal stem cells according to claim 1, characterized in that, The rotating ball (5) has a groove on its side wall.

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