A microbial filtration device

Abstract

The utility model discloses a kind of microbial filtration devices, including base, filter cup, filter membrane, gasket, conduit and liquid outlet sealing cover;The filter cup is provided with liquid inlet and air inlet;The base is equipped with liquid outlet, the top of the base is provided with recess, and the liquid outlet is arranged in the bottom of the recess;The filter membrane and gasket are sequentially placed from top to bottom in the recess, and the filter cup is detachably mounted on the base.The microbial filtration device determines the filtration state and the cultivation state by different assembly modes, without opening the device or transferring the filter membrane to another petri dish, the nutrient agar can be directly injected into the microbial filtration device through the liquid outlet, and the cultivation can be carried out, solving the risk of contamination of the filter membrane exposed to a non-sterile environment during the transfer process, simplifying the operation process, and greatly improving the accuracy of the experiment.

Description

Technical Field

[0001] This utility model relates to the field of microbial collection and detection equipment, and in particular to a microbial filtration device. Background Technology

[0002] Currently, after endoscopes are disinfected, they need to be tested for microorganisms to ensure they are sterile. The testing steps mainly include: injecting eluent into the endoscope and collecting any microorganisms that may be present; then isolating and culturing the microorganisms in the eluent; and finally, determining whether there are still microbial residues in the endoscope by calculating the colony count.

[0003] Currently, microbial filtration devices have been developed that can filter eluents, trapping microorganisms on a filter membrane for easy cultivation. However, in existing technologies, the steps of filtering the eluent and incorporating the filter membrane into the culture medium are performed using separate containers. This separation between processes often leads to bacterial contamination, affecting the accuracy of bacterial counts and results. Utility Model Content

[0004] The purpose of this invention is to propose a microbial filtration device to solve the problem that existing microbial filtration devices have the risk of foreign bacteria infecting the filter membrane, leading to inaccurate results.

[0005] To achieve this objective, the present invention adopts the following technical solution: This utility model provides a microbial filtration device, which includes a base, a filter cup, a filter membrane, a gasket, a conduit, and a liquid outlet sealing cap; The filter cup is provided with a liquid inlet and an air inlet; the base is provided with a liquid outlet, and the top of the base is provided with a groove, with the liquid outlet located at the bottom of the groove; the filter membrane and the gasket are placed sequentially from top to bottom in the groove, and the filter cup is detachably installed on the base, pressing the filter membrane and the gasket tightly in the groove; the top of the filter cup is provided with three or more support columns, which are circumferentially spaced with the axis of the filter cup as the central axis; The microbial filtration device includes a filtration state and a cultivation state. When the microbial filtration device is in the filtration state, the liquid inlet is connected to one end of the conduit, and the liquid outlet is connected to an external drainage device. When the microbial filtration device is in the cultivation state, the liquid inlet is connected to one end of the conduit, and the air inlet is connected to the other end of the conduit. The liquid outlet sealing cap is detachably installed at the liquid outlet.

[0006] In the microbial filtration device, the filter cup includes a filter cup body and a filter cup cover. The filter cup cover is detachably connected to the top opening of the filter cup body, and the bottom of the filter cup body is detachably connected to the base. The support column, liquid inlet, and air inlet are all located on the filter cup cover. The air inlet is centrally located in the middle of the filter cup cover. Several support columns are circumferentially arranged around the outer periphery of the air inlet, and the length of the support column is greater than the length of the air inlet. The liquid inlet is located on one side of the air inlet.

[0007] In the microbial filtration device, the filter cup body and the filter cup cover are threadedly connected.

[0008] In the microbial filtration device, the liquid inlet port penetrates the inner wall of the filter cup lid and protrudes to one side of the filter cup body.

[0009] In the microbial filtration device, the base is provided with a protrusion, the bottom of the filter cup body extends downward with a fixing ring, the fixing ring is provided with a sliding groove, and a snap-fit ​​notch communicating with the sliding groove is opened below the sliding groove. The protrusion enters the sliding groove from the snap-fit ​​notch and moves along the sliding groove to the closed end of the sliding groove. The filter cup and the base are connected by a rotating snap-fit ​​structure.

[0010] In the microbial filtration device, an air filter membrane is installed at the air inlet.

[0011] In the microbial filtration device, the filter cup is also provided with a sample retention interface that communicates with the inner cavity of the filter cup. The connection between the sample retention interface and the filter cup is located between the liquid inlet interface and the filter membrane. The microbial filtration device further includes a sample cup, a sample cup sealing cap, and a sample port sealing cap; when the microbial filtration device is in filtration mode, the sample cup is detachably connected to the sample port; when the microbial filtration device is in culture mode, the sample cup sealing cap is detachably connected to the sample cup, and the sample port sealing cap is placed on the sample port.

[0012] In the microbial filtration device, a fixing plate extends outward from the bottom of the base.

[0013] One of the technical solutions of this utility model can have the following beneficial effects: The microbial filtration device determines the filtration and cultivation states through different assembly methods. Without opening the device or transferring the filter membrane to another petri dish, nutrient agar can be directly injected into the microbial filtration device through the outlet for cultivation. This eliminates the risk of contamination of the filter membrane due to exposure to a non-sterile environment during membrane transfer, simplifies the operation process, and makes it faster and more convenient. It also greatly improves the accuracy of experiments; furthermore, it saves petri dishes and culture media, reducing consumable consumption. Attached Figure Description

[0014] Figure 1 This is a schematic diagram of the structure of one embodiment of the present invention in a filtering state; Figure 2 This is a cross-sectional schematic diagram of one embodiment of the present invention in the cultivation state; In the attached diagram: 1. Base; 2. Filter cup; 3. Filter membrane; 4. Gasket; 5. Guide tube; 6. Liquid outlet sealing cap. 11. Protrusion; 12. Fixing plate; 21. Filter cup body; 22. Filter cup cover; 23. Sample retention cup; 24. Sample retention cup sealing cover; 25. Sample retention port sealing cover; Liquid outlet 101; liquid inlet 201, air inlet 202; support column 203; air filter membrane 204; sample retention interface 205; fixing collar 211; sliding groove 212, snap-fit ​​notch 213. Detailed Implementation

[0015] The embodiments of this utility model are described in detail below. Examples of these embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain this utility model, and should not be construed as limiting this utility model.

[0016] In the description of this utility model, it should be understood that the terms "longitudinal," "lateral," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," and "outer," etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the accompanying drawings and are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model. Furthermore, features defined with "first" and "second" may explicitly or implicitly include one or more of these features, used to distinguish descriptive features, without any order or emphasis.

[0017] In the description of this utility model, unless otherwise stated, "a plurality of" means two or more.

[0018] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0019] Please refer to Figure 1 and 2 This utility model provides a microbial filtration device, which includes a base 1, a filter cup 2, a filter membrane 3, a gasket 4, a conduit 5, and an outlet sealing cap 6. The filter cup 2 is provided with a liquid inlet 201 and an air inlet 202; the base 1 is provided with a liquid outlet 101, and the top of the base 1 is provided with a groove, the liquid outlet 101 being located at the bottom of the groove; the filter membrane 3 and the gasket 4 are placed sequentially from top to bottom in the groove, the filter cup 2 is detachably mounted on the base 1, and the filter membrane 3 and the gasket 4 are pressed tightly in the groove; the top of the filter cup 2 is provided with three or more support columns 203, the support columns 203 being circumferentially spaced with the axis of the filter cup 2 as the central axis; The microbial filtration device includes a filtration state and a cultivation state. When the microbial filtration device is in the filtration state, the liquid inlet 201 is connected to one end of the conduit 5, and the liquid outlet 101 is connected to an external drainage device. When the microbial filtration device is in the cultivation state, the liquid inlet 201 is connected to one end of the conduit 5, and the air inlet 202 is connected to the other end of the conduit 5. The liquid outlet sealing cap 6 is detachably installed on the liquid outlet 101.

[0020] The drainage device includes a drainage pump and a drainage pipe. One end of the drainage pump is connected to the drainage pipe, and the other end of the drainage pipe is connected to the outlet 101. When the drainage pump is started, it creates a negative pressure, thereby drawing the eluent from the base 1 and filter cup 2 through the outlet 101. The eluent then passes through the filter membrane 3 and the gasket 4 to achieve filtration. The working process of the microbial filtration device includes: injecting the eluent into the endoscope biopsy port, and then collecting the entire eluent; first connecting the conduit 5 to the inlet port 201 and the drainage pipe to the outlet 101, at which point the microbial filtration device is in filtration mode; then introducing the eluent into the conduit 5; under the action of an external peristaltic pump, the eluent enters the microbial filtration device from the conduit 5; then, under the action of the external drainage device, the eluent flows out from the drainage pipe after being filtered by the filter membrane 3, and the microorganisms in the liquid are intercepted on the filter membrane 3.

[0021] The eluent can enter the outlet 101 through the gasket 4. The gasket 4 can slow down the speed at which the eluent enters the outlet 101, distribute the pressure evenly, and prevent the fragile filter membrane 3 from being crushed or worn by the rigid support plate below, thereby protecting the integrity of the filter membrane.

[0022] After the eluent is completely filtered, the other end of the conduit 5 is installed at the air inlet 202; then the microbial filtration device is inverted so that the support column 203 supports the entire microbial filtration device, thereby placing the base 1 above the filter cup 2 and exposing the liquid outlet 101; liquid nutrient agar is injected into the liquid outlet 101; after injection, the liquid outlet sealing cap 6 is installed on the liquid outlet 101, and then the microbial filtration device is reset so that the filter cup 2 is above the base 1. At this time, the microbial filtration device is in the cultivation state.

[0023] The microbial filtration device determines the filtration and cultivation states through different assembly methods. Without opening the device or transferring the filter membrane to another petri dish, nutrient agar can be directly injected into the microbial filtration device through the outlet 101 for cultivation. This eliminates the risk of contamination of the filter membrane 3 due to exposure to a non-sterile environment during membrane transfer, simplifies the operation process, and makes it faster and more convenient. It also greatly improves the accuracy of experiments; furthermore, it saves petri dishes and culture media, reducing consumable consumption.

[0024] Specifically, the filter cup 2 includes a filter cup body 21 and a filter cup cover 22. The filter cup cover 22 is detachably connected to the top opening of the filter cup body 21, and the bottom of the filter cup body 21 is detachably connected to the base 1. The support column 203, the liquid inlet 201, and the air inlet 202 are all disposed on the filter cup cover 22. The air inlet 202 is centrally disposed in the middle of the filter cup cover 22. Several support columns 203 are circumferentially disposed on the outer periphery of the air inlet 202, and the length of the support column 203 is greater than the length of the air inlet 202. The liquid inlet 201 is disposed on one side of the air inlet 202.

[0025] With the above structure, the filter cup body 21 and the filter cup cover 22 can be detached and installed, facilitating the cleaning and sterilization of the filter cup 2. The length of the support column 203 is greater than the length of the air inlet 202. When the support column 203 supports the entire microbial filtration device, it can stably support the device, preventing the top of the filter cup 2 from directly contacting the bottom surface of the incubator and preventing the air inlet sealing cover from being squeezed, which could lead to a breach in the seal.

[0026] Specifically, the filter cup body 21 is threadedly connected to the filter cup cover 22.

[0027] In one specific embodiment of this utility model, the filter cup body 21 and the filter cup cover 22 are detachably installed via threads. This structure facilitates the assembly and disassembly of the filter cup body 21 and the filter cup cover 22, while ensuring good sealing during assembly.

[0028] Specifically, the liquid inlet 201 penetrates the inner wall of the filter cup cover 22 and protrudes to one side of the filter cup body 21.

[0029] With the above structure, the liquid inlet 201 protrudes from the inner wall of the filter cup body 21 to one side, which avoids the problem of nutrient agar flowing into the liquid inlet 201 when the filter cup is inverted, causing the nutrient agar to harden and block the liquid inlet 201.

[0030] Specifically, the base 1 is provided with a protrusion 11, and the bottom of the filter cup body 21 extends downward with a fixing ring 211. The fixing ring 211 is provided with a sliding groove 212. A snap-fit ​​notch 213 communicating with the sliding groove 212 is provided below the sliding groove 212. The protrusion 11 enters the sliding groove 212 from the snap-fit ​​notch 213 and moves along the sliding groove 212 to the closed end of the sliding groove 212. The filter cup 2 and the base 1 are connected by a rotating snap-fit ​​structure.

[0031] With the above structure, simply align the fixing ring 211 on the filter cup 2 with the protrusion 11 on the base 1, insert it downwards into the snap-fit ​​notch 213, and then gently rotate the filter cup 2. The protrusion 11 will slide along the groove to the closed end to complete the connection. Disassembly can be done by rotating in the opposite direction, which achieves the purpose of quick installation and disassembly and is easy to operate.

[0032] In one specific embodiment of this utility model, a sealing ring is provided between the filter cup 2 and the base 1, and the sealing ring is located above the filter membrane 3. During rotation, a uniform clamping force is applied to the sealing ring between the filter cup 2 and the base 1, thereby ensuring the sealing of the connection.

[0033] Specifically, the air inlet 202 is equipped with an air filter membrane 204.

[0034] The base 1 and filter cup 2 work together to form a filter chamber, and the air inlet 202 is used to balance the air pressure between the filter chamber and the outside air during the filtration process. This structure avoids the problem of airborne bacteria being drawn into the filter chamber during filtration, thus preventing contamination and reducing the likelihood of inaccurate test results.

[0035] Optionally, the filter cup 2 is also provided with a sample retention port 205 that communicates with the inner cavity of the filter cup 2. The connection between the sample retention port 205 and the filter cup 2 is located between the liquid inlet port 201 and the filter membrane 3. The microbial filtration device further includes a sample cup 23, a sample cup sealing cap 24, and a sample port sealing cap 25; when the microbial filtration device is in the filtration state, the sample cup 23 is detachably connected to the sample port 205; when the microbial filtration device is in the cultivation state, the sample cup sealing cap 24 is detachably connected to the sample cup 23, and the sample port sealing cap 25 is placed on the sample port 205.

[0036] With the above structure, the testing personnel can retain a portion of the eluent through the sample retention cup 23 for sample recording. Before the microbial filtration device switches from filtration to culture, the sample retention cup 23 must be removed from the sample retention port 205 and the sample retention cup sealing cap 24 should be quickly placed on it for sealed preservation. At the same time, the sample retention port sealing cap 25 should be fixed to the sample retention port 205.

[0037] Preferably, a fixing plate 12 extends outward from the bottom of the base 1.

[0038] By adopting the above structure, the contact area between the base 1 and the table is increased by the fixing plate 12, forming a more stable support, reducing the possibility of the microbial filtration device tipping over due to accidental collisions, and improving the overall stability.

[0039] The technical principles of this utility model have been described above with reference to specific embodiments. These descriptions are merely for explaining the principles of this utility model and should not be construed as limiting the scope of protection of this utility model in any way. Based on this explanation, those skilled in the art can readily conceive of other specific embodiments of this utility model without inventive effort, and these equivalent modifications or substitutions are all included within the scope defined by the claims of this application.

Claims

1. A microbial filtration device, characterized in that, The microbial filtration device includes a base, a filter cup, a filter membrane, a gasket, a conduit, and a liquid outlet sealing cap; The filter cup is provided with a liquid inlet and an air inlet; the base is provided with a liquid outlet, and the top of the base is provided with a groove, with the liquid outlet located at the bottom of the groove; the filter membrane and the gasket are placed sequentially from top to bottom in the groove, and the filter cup is detachably installed on the base, pressing the filter membrane and the gasket tightly in the groove; the top of the filter cup is provided with three or more support columns, which are circumferentially spaced with the axis of the filter cup as the central axis; The microbial filtration device includes a filtration state and a cultivation state. When the microbial filtration device is in the filtration state, the liquid inlet is connected to one end of the conduit, and the liquid outlet is connected to an external drainage device. When the microbial filtration device is in the cultivation state, the liquid inlet is connected to one end of the conduit, and the air inlet is connected to the other end of the conduit. The liquid outlet sealing cap is detachably installed at the liquid outlet.

2. The microbial filtration device according to claim 1, characterized in that, The filter cup includes a filter cup body and a filter cup cover. The filter cup cover is detachably connected to the top opening of the filter cup body, and the bottom of the filter cup body is detachably connected to the base. The support column, liquid inlet, and air inlet are all located on the filter cup cover. The air inlet is centrally located in the middle of the filter cup cover. Several support columns are circumferentially arranged around the outer periphery of the air inlet, and the length of the support column is greater than the length of the air inlet. The liquid inlet is located on one side of the air inlet.

3. A microbial filtration device according to claim 2, characterized in that, The filter cup body is threadedly connected to the filter cup cover.

4. A microbial filtration device according to claim 2, characterized in that, The liquid inlet port penetrates the inner wall of the filter cup cover and protrudes to one side of the filter cup body.

5. A microbial filtration device according to claim 2, characterized in that, The base has a protrusion, and the bottom of the filter cup body extends downward with a fixing ring. The fixing ring has a sliding groove, and a snap-fit ​​notch communicating with the sliding groove is opened below the sliding groove. The protrusion enters the sliding groove from the snap-fit ​​notch and moves along the sliding groove to the closed end of the sliding groove. The filter cup and the base are connected by a rotating snap-fit ​​structure.

6. A microbial filtration device according to claim 1, characterized in that, An air filter membrane is installed at the air inlet.

7. A microbial filtration device according to claim 1, characterized in that, The filter cup is also provided with a sample retention port that communicates with the inner cavity of the filter cup. The connection between the sample retention port and the filter cup is located between the liquid inlet port and the filter membrane. The microbial filtration device further includes a sample cup, a sample cup sealing cap, and a sample port sealing cap; when the microbial filtration device is in filtration mode, the sample cup is detachably connected to the sample port; when the microbial filtration device is in culture mode, the sample cup sealing cap is detachably connected to the sample cup, and the sample port sealing cap is placed on the sample port.

8. A microbial filtration device according to claim 1, characterized in that, The base has a fixing plate extending outward from its bottom.

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