A hand-held portable constant temperature amplification detection device

Abstract

The utility model belongs to detection device technical field, concretely relates to a hand -held portable constant temperature amplification detection device, include: amplification pipe, card box and hatcher, the amplification pipe includes amplification pipe A and amplification pipe B, amplification pipe A includes the reaction bin and the breakage groove at reaction bin bottom, be provided with magnetic stirring in the reaction bin, the upper portion of reaction bin is provided with internal thread, and the outer wall is provided with first outer thread still, amplification pipe B includes the pipe head that is provided with second outer thread, to cooperate the internal thread of amplification pipe A and carry out the connection, be provided with valve port and the broken rod that one end sets up in valve port on the pipe head, be provided with soft rubber tube below valve port, the initial state of valve port is closed, and after the broken rod in soft rubber tube stress deformation breaks, valve port opens, the pipe head and soft rubber tube are connected through fixed snap ring between and form the seal.

Description

Technical Field

[0001] This utility model belongs to the technical field of detection devices, specifically relating to a handheld portable isothermal amplification detection device. Background Technology

[0002] In the field of nucleic acid testing, many similar products on the market use fluorescence detection PCR solutions. Fluorescence detection PCR solutions require complex opto-mechanical instruments, have a high degree of automation, and high throughput, but the instrument cost and consumable cost are also high, making them unsuitable for low-throughput, low-cost applications such as home environments or primary care testing. Another type of immunoassay device based on lateral flow strips has a short detection time and is simple to operate, but because it lacks a nucleic acid amplification step, its sensitivity is lower and it may miss low concentrations of target substances, but it can still achieve qualitative judgment of positive / negative results.

[0003] In our existing patent CN202320104241.5, there is a home nucleic acid testing device, in which the incubator can meet the basic functional requirements, but its consumables are relatively cumbersome to operate and there is insufficient space for hand operation.

[0004] Therefore, overcoming the shortcomings of existing handheld isothermal amplification detection devices, which are too large and inconvenient for hand operation, is a technical problem that urgently needs to be solved in this field.

[0005] It should be noted that the information disclosed in this background section is only for understanding the background technology of the present application concept, and therefore, the above description is not considered to constitute prior art information. Summary of the Invention

[0006] This disclosure provides at least one handheld portable isothermal amplification detection device.

[0007] In a first aspect, this disclosure provides a handheld portable isothermal amplification detection device, comprising: an amplification tube, a cartridge, and an incubator; the amplification tube includes amplification tube A and amplification tube B, amplification tube A includes a reaction chamber and a rupture groove located at the bottom of the reaction chamber, a magnetic stir bar is provided inside the reaction chamber, an internal thread is provided on the upper part of the reaction chamber, and a first external thread is provided on the outer wall; amplification tube B includes a tube head with a second external thread for connection with the internal thread of amplification tube A, a valve port and a break rod with one end provided at the valve port are provided on the tube head, a soft rubber tube is provided below the valve port, the valve port is initially closed, and the valve port opens after the break rod is deformed and broken by the soft rubber tube under force, the tube head and the soft rubber tube are connected and sealed by a retaining ring; an aluminum groove is provided above the incubator for inserting into the reaction chamber for amplification reaction; an amplification tube insertion port is provided above the cartridge for inserting into the reaction chamber for colorimetric reaction.

[0008] In one optional embodiment, a hand-tightening strip is also provided on the outer side of the internal thread of the amplification tube A.

[0009] In one optional embodiment, the incubator includes a main control board located at the bottom of the incubator; a temperature control sensor is embedded in the bottom of the aluminum tank and electrically connected to the main control board; the temperature control sensor is connected to a temperature control protection switch.

[0010] In one optional embodiment, a DC motor is disposed below the aluminum tank. The DC motor is connected to the outer shell of the incubator via an LED light control board. Two magnets with opposite magnetic pole directions are disposed on the shaft of the DC motor to drive the magnetic stir bar in the reaction chamber to rotate when the DC motor rotates. The DC motor is electrically connected to the main control board.

[0011] In one optional embodiment, the incubator's outer casing is further provided with multi-color LED lights and a ring-shaped lampshade for lighting indication; the multi-color LED lights and the ring-shaped lampshade are electrically connected to the LED light control board, and the LED light control board is electrically connected to the main control board.

[0012] In one alternative implementation, the incubator further includes a voice module and a Type-C interface, which are integrated into and electrically connected to a main control board located at the bottom of the incubator.

[0013] In one optional embodiment, the cartridge includes an upper cartridge and a lower cartridge; the upper cartridge is provided with an amplification tube socket and a viewing window, and the amplification tube socket is provided with a second internal thread that engages with a first external thread.

[0014] In one optional embodiment, the lower box portion is provided with a piercing rod and a liquid storage tank. The piercing rod is used to pierce the crack groove at the bottom of the reaction chamber and allow the reaction liquid to flow into the liquid storage tank, and then through the drainage channel to allow the reaction liquid to flow into the paper strip groove.

[0015] In one optional embodiment, a flow interceptor is provided between the storage tank and the diversion channel to control the flow rate of the reaction liquid.

[0016] In one optional embodiment, an overflow groove is provided below the paper strip groove to prevent the reaction liquid from overflowing into the paper strip groove.

[0017] The beneficial effects of this utility model are that the handheld portable isothermal amplification detection device is designed with separate tubes A and B in the amplification tube, and the reaction chamber and the break rod are structurally detachable in a sealed environment, which solves the problem of insufficient hand operation space, simplifies the operation steps and reduces the difficulty of the production process, and provides a technical solution for use by people with no prior experience.

[0018] Other features and advantages of this invention will be set forth in the description which follows, and will be apparent in part from the description, or may be learned by practicing the invention. The objectives and other advantages of this invention are realized and obtained through the structures particularly pointed out in the description and drawings.

[0019] To make the above-mentioned objectives, features and advantages of this utility model more apparent and understandable, preferred embodiments are described below in detail with reference to the accompanying drawings. Attached Figure Description

[0020] To more clearly illustrate the specific embodiments of this utility model or the technical solutions in the prior art, the drawings used in the description of the specific embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of this utility model. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0021] Figure 1 This is a schematic diagram of the structure of the amplification tube in the handheld portable isothermal amplification detection device provided in the embodiments of this disclosure; Figure 2 A cross-sectional view of the amplification tube in the handheld portable isothermal amplification detection device provided in the embodiments of this disclosure; Figure 3 This is a schematic diagram of the cartridge structure in the handheld portable isothermal amplification detection device provided in the embodiments of this disclosure; Figure 4 This is a schematic diagram of the incubator in the handheld portable isothermal amplification detection device provided in the embodiments of this disclosure; Figure 5 This is a schematic diagram of amplification in a handheld portable isothermal amplification detection device provided in an embodiment of this disclosure; Figure 6 This is a schematic diagram of color development in a handheld portable isothermal amplification detection device provided in an embodiment of this disclosure.

[0022] In the picture: 100. Amplification tube A; 101. Reaction chamber; 102. Rupture groove; 103. Internal thread; 104. First external thread; 105. Hand-tightening strip; 200. Amplification tube B; 201. Valve port; 202. Breaking rod; 203. Flexible tubing; 204. Retaining clasp; 205. Second external thread; 210. Tube end; 300. Card holder; 301. Amplification tube inlet; 302. Second internal thread; 303. Viewing window; 304. Liquid reservoir; 305. Drainage channel; 306. Paper strip groove; 307. Interception groove; 308. Overflow groove; 309. Puncture rod; 400. Incubator; 401. Aluminum tank; 402. Temperature sensor; 403. Temperature protection switch; 404. DC motor; 405. LED light control board; 406. Magnet; 407. Multi-color LED light; 408. Ring lampshade; 410. Main control board; 411. Type-C interface; 412. Voice module. Detailed Implementation

[0023] To make the objectives, technical solutions, and advantages of the embodiments of this utility model clearer, the technical solutions of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this utility model, not all embodiments. Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of this utility model.

[0024] As used herein, the phrases “in one embodiment,” “according to one embodiment,” “in some embodiments,” etc., generally refer to the fact that a particular feature, structure, or characteristic following the phrase can be included in at least one embodiment of this disclosure. Therefore, a particular feature, structure, or characteristic can be included in more than one embodiment of this disclosure, such that these phrases do not necessarily refer to the same embodiment. As used herein, the terms “example,” “exemplary,” etc., are used to “serve as an example, instance, or illustration.” Any implementation, aspect, or design described herein as “example” or “exemplary” is not necessarily to be construed as preferred or superior to other implementations, aspects, or designs. Rather, the use of the terms “example,” “exemplary,” etc., is intended to present concepts in a specific manner.

[0025] In this document, exemplary embodiments of the present disclosure will be described in more detail with reference to the accompanying drawings. As used herein, expressions such as “at least one of…” modify the entire list of elements when following a list of elements, rather than individual elements in the list. For example, the expression “at least one of a, b, and c” should be understood to include only a, only b, only c, both a and b, both a and c, both b and c, or all of a, b, and c.

[0026] The terminology used herein is for the purpose of describing specific exemplary configurations only and is not intended to be limiting. As used herein, the singular articles “a,” “an,” and “the” may also be intended to include plural forms unless otherwise clearly stated herein. The terms “comprising,” “including,” and “having” are inclusive and thus specify the presence of features, steps, operations, elements, and / or components, but do not preclude the presence or addition of one or more other features, steps, operations, elements, components, and / or combinations thereof. The method steps, processes, and operations described herein should not be construed as requiring them to be performed in the specific order discussed or shown, unless specifically identified as such. Additional or alternative steps may be employed.

[0027] It should be noted that similar labels and letters in the following figures indicate similar items. Therefore, once an item is defined in one figure, it does not need to be further defined and explained in subsequent figures.

[0028] The following detailed description, with reference to the accompanying drawings, describes some embodiments of the present invention. Unless otherwise specified, the following embodiments and features can be combined with each other.

[0029] Please see Figure 1 and Figure 2 As shown, this disclosure provides a handheld portable isothermal amplification detection device, including: amplification tubes, a cartridge 300, and an incubator 400; the amplification tubes include amplification tube A 100 and amplification tube B 200, amplification tube A 100 includes a reaction chamber 101 and a rupture groove 102 located at the bottom of the reaction chamber 101, a magnetic stir bar is provided inside the reaction chamber 101, the upper part of the reaction chamber 101 is provided with an internal thread 103, and the outer wall is also provided with a first external thread 104; the amplification tube B 200 includes a tube head 210 provided with a second external thread 205 to cooperate with amplification tube A. The pipe head 210 is connected by an internal thread 103. A valve port 201 and a breakable rod 202 with one end attached to the valve port 201 are provided on the pipe head 210. A flexible rubber tube 203 is located below the valve port 201. The valve port 201 is initially closed. After the breakable rod 202 breaks due to deformation of the flexible rubber tube 203, the valve port 201 opens. The pipe head 210 and the flexible rubber tube 203 are connected and sealed by a retaining ring 204. Figure 5 As shown, an aluminum groove is provided above the incubator for inserting the reaction chamber 101 to carry out the amplification reaction; as Figure 6 As shown, the card box has an amplification tube socket on its top for insertion into the reaction chamber 101 for colorimetric reaction.

[0030] Specifically, the reaction chamber 101 can encapsulate one or more lyophilized reagent microspheres, lyophilized reagent powders, or liquid reagents. The reaction chamber 101 can be made of PP plastic. The bottom of the reaction chamber 101 is thin and the sides are thick, which facilitates the cooperation between the rupture groove 102 and the puncture rod 309.

[0031] Specifically, the valve port 201 is initially closed. When the flexible tube 203 is deformed by manual operation, causing the break rod 202 to bend and break, the valve port 201 opens, and the flexible tube 203 flows out through the valve port 201. The retaining ring 204 is located on the outside of the flexible tube 203, and a seal is formed between the flexible tube 203 and the tube head 210 by assembly compression.

[0032] In some embodiments, specifically, a hand-tightening strip 105 is provided on the outside of the internal thread 103 of the amplification tube A 100.

[0033] In some embodiments, specifically, the incubator 400 includes a main control board 410 located at the bottom of the incubator; a temperature control sensor 402 is embedded in the bottom of the aluminum tank 401 and electrically connected to the main control board 410; the temperature control sensor 402 is connected to a temperature control protection switch 403; the function of the temperature sensor 402 is to detect the temperature of the aluminum tank in real time and control the temperature by adjusting the temperature parameters. Optionally, the temperature is set to 42°C, the heating film is activated, and when the temperature of the aluminum tank reaches 42°C, the temperature sensor feeds the detection data back to the main board circuit board, controlling the heating film to stop heating and enter natural cooling. When the aluminum bath temperature cools to 41℃, the temperature sensor feeds the detected data back to the main board circuit board, controlling the heating film to start heating. This cycle repeats to ensure precise temperature control. The temperature protection switch 403 is for over-temperature protection. When the temperature sensor fails, due to the lack of real-time temperature data or erroneous data feedback to the main board circuit board, the heating film will continue to start heating without stopping. Overheating can lead to device malfunction and the risk of high temperature. At this time, the temperature protection switch will activate, directly disconnecting the power supply to the heating film and stopping heating. Optionally, a 55℃ model temperature protection switch can be selected. When the aluminum bath temperature exceeds 55℃, the power to the heating film will be cut off.

[0034] In some embodiments, specifically, a DC motor 404 is disposed below the aluminum tank 401. The DC motor 404 is connected to the outer shell of the incubator 400 through an LED light control board 405. Two magnets 406 with opposite magnetic pole directions are disposed on the shaft of the DC motor 404 so that when the DC motor 404 rotates, it drives the magnetic stir bar in the reaction chamber 101 to rotate. When the DC motor 404 rotates, it drives the magnets 406 to rotate, forming a rotating magnetic field in the aluminum tank 401. The stir bar in the amplification tube rotates under the influence of the rotating magnetic field, thereby stirring and mixing the reagent liquid. The DC motor 404 is electrically connected to the main control board 410.

[0035] In some embodiments, specifically, the incubator 400 is further provided with a multi-color LED light 407 and a ring lampshade 408 for lighting indication; the multi-color LED light 407 and the ring lampshade 408 are electrically connected to the LED light control board 405, and the LED light control board 405 is electrically connected to the main control board 410.

[0036] In some embodiments, the incubator 400 further includes a voice module 412 and a Type-C interface 411, which are integrated on and electrically connected to a main control board 410 located at the bottom of the incubator 400.

[0037] Specifically, because this handheld portable isothermal amplification detection device has indicator lights and voice prompts, it guides users through the entire nucleic acid amplification reaction process, further reducing user learning and training costs.

[0038] Specifically, as an optional implementation method, the control timing is as follows: S1, heating period, the temperature rises from 37℃ to 42℃, and the mixing process is carried out from 0-20s, with the light showing alternating yellow and blue. The rapid heating process is carried out from 20-120s, with the light showing a constant yellow. S2, constant temperature incubation period, heated at 42℃ for 20 minutes, the light is yellow and constantly bright; S3, after the natural cooling process ends, the temperature drops from 42℃ to 30℃. First, it is left to cool down for 3 minutes, during which the light flashes yellow quickly. Then, it undergoes a 20-second mixing process, during which the light alternates between yellow and blue. Finally, it cools down naturally to 30℃, during which the light is constantly blue and enters standby mode.

[0039] Please see Figure 3 ,like Figure 3 As shown, specifically, the card box 300 includes an upper box and a lower box; the upper box is provided with an amplification tube socket 301 and a window 303, and the amplification tube socket is provided with a second internal thread 302 and is connected to the first external thread 104.

[0040] In some embodiments, specifically, the lower box portion is provided with a piercing rod 309 and a liquid storage tank 304. The piercing rod 309 is used to pierce the rupture groove 102 at the bottom of the reaction chamber 101 and allow the reaction liquid to flow into the liquid storage tank 304, and then through the drainage channel 305 to allow the reaction liquid to flow into the paper strip groove 306. Optionally, the drainage channel 305 is 2.5 mm wide.

[0041] Specifically, the cartridge 300 can contain one or more colloidal gold test strips, enabling single- to multiplex nucleic acid amplification detection in a single experiment. It can be configured according to the user's experimental needs to meet different application scenarios. In some embodiments, specifically, a flow interceptor 307 is provided between the storage tank and the diversion channel, the flow interceptor being 1.9 mm wide, to control the flow rate of the reaction liquid.

[0042] In some embodiments, specifically, an overflow groove 308 is provided below the paper strip groove to prevent the reaction liquid from overflowing in the paper strip groove.

[0043] In summary, this handheld portable isothermal amplification detection device features separate A and B tubes in its amplification tube design. The reaction chamber and break rod are designed to be detachable in a sealed environment, solving the problem of insufficient hand operating space. It also simplifies the operation steps and reduces the difficulty of the manufacturing process, providing a technical solution for users with no prior experience.

[0044] Based on the above-described preferred embodiments of this utility model, and through the foregoing description, those skilled in the art can make various changes and modifications without departing from the technical concept of this utility model. The technical scope of this utility model is not limited to the contents of the specification, but must be determined according to the scope of the claims.

Claims

1. A handheld portable isothermal amplification detection device, characterized in that, include: Amplification tubes, cartridges (300), and incubators (400); The amplification tube includes amplification tube A (100) and amplification tube B (200). Amplification tube A (100) includes a reaction chamber (101) and a rupture groove (102) located at the bottom of the reaction chamber (101). A magnetic stir bar is provided inside the reaction chamber (101). An internal thread (103) is provided on the upper part of the reaction chamber (101), and a first external thread (104) is provided on the outer wall. The amplification tube B (200) includes a tube head (210) with a second external thread (205) for connection with the internal thread (103) of the amplification tube A (100). The tube head (210) is provided with a valve port (201) and a break rod (202) with one end provided at the valve port (201). A soft rubber tube (203) is provided below the valve port (201). The valve port (201) is initially closed. After the break rod (202) is broken by the soft rubber tube (203) under force deformation, the valve port (201) opens. The tube head (210) and the soft rubber tube (203) are connected and sealed by a retaining ring (204). An aluminum groove is provided above the incubator for inserting the reaction chamber (101) for amplification reaction; The card box is provided with an amplification tube socket on the top for insertion into the reaction chamber (101) for colorimetric reaction.

2. The handheld portable isothermal amplification detection device as described in claim 1, characterized in that, The amplification tube A (100) is also provided with a hand-tightening strip (105) on the outside of the internal thread (103).

3. The handheld portable isothermal amplification detection device as described in claim 1, characterized in that, The incubator (400) includes a main control panel (410) located at the bottom of the incubator; A temperature control sensor (402) is embedded in the bottom of the aluminum tank and is electrically connected to the main control board (410); The temperature control sensor (402) is connected to a temperature control protection switch (403).

4. The handheld portable isothermal amplification detection device as described in claim 3, characterized in that, A DC motor (404) is installed below the aluminum tank (401). The DC motor (404) is connected to the outer shell of the incubator through an LED light control board (405). Two magnets (406) with opposite magnetic pole directions are installed on the shaft of the DC motor (404) so ​​as to drive the magnetic stir bar in the reaction chamber (101) to rotate when the DC motor (404) rotates. The DC motor (404) is electrically connected to the main control board (410).

5. The handheld portable isothermal amplification detection device as described in claim 4, characterized in that, The incubator (400) is also equipped with multi-color LED lights (407) and a ring-shaped lampshade (408) for lighting indication; The multi-color LED lamp (407) and the ring lamp cover (408) are electrically connected to the LED lamp control board (405), and the LED lamp control board (405) is electrically connected to the main control board (410).

6. The handheld portable isothermal amplification detection device as described in claim 1, characterized in that, The incubator also includes a voice module (412) and a Type-C interface (411), which are integrated on and electrically connected to the main control board (410) located at the bottom of the incubator.

7. The handheld portable isothermal amplification detection device as described in claim 1, characterized in that, The card box (300) includes an upper box and a lower box; The upper box is provided with an amplification tube socket (301) and a window (303). The amplification tube socket is provided with a second internal thread (302) and is connected to the first external thread (104).

8. The handheld portable isothermal amplification detection device as described in claim 7, characterized in that, The lower box is provided with a piercing rod (309) and a liquid storage tank (304). The piercing rod (309) is used to pierce the crack groove (102) at the bottom of the reaction chamber (101) and allow the reaction liquid to flow into the liquid storage tank (304), and then through the drainage channel (305) to allow the reaction liquid to flow into the paper strip groove (306).

9. The handheld portable isothermal amplification detection device as described in claim 8, characterized in that, A flow interceptor (307) is provided between the storage tank and the diversion channel to control the flow rate of the reaction liquid.

10. The handheld portable isothermal amplification detection device as described in claim 8, characterized in that, An overflow trough (308) is provided below the paper strip groove to prevent the reaction liquid from overflowing into the paper strip groove.

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