A high-throughput cell sorting magnetic bead recovery device

Abstract

The utility model relates to cell sorting technical field, concretely to a kind of high-throughput cell sorting magnetic bead recovery device, including placing seat, the top of placing seat is downwardly provided with mounting groove, storage tube is installed in mounting groove, storage tube inside has storage cavity, storage cavity is used to store cell sorting test tube, the outside of storage tube is provided with electromagnetic coil, after electromagnetic coil energization, reaction magnetic field is formed inside storage cavity, the outside of electromagnetic coil is provided with shield cover, inner heat conduction structure is arranged between shield cover and electromagnetic coil, the outside of shield cover is provided with outer heat conduction structure, the top of shield cover is provided with limit along, the top of storage tube is provided with mounting along, mounting along is detachably engaged in mounting groove, and located above limit along, the utility model can be flexibly adapted to the separation of magnetic bead of different magnetism, use more flexible.

Description

Technical Field

[0001] This utility model relates to the field of cell sorting technology, specifically a high-throughput cell sorting magnetic bead recovery device. Background Technology

[0002] In the field of cell sorting technology, magnetic bead sorting is widely used due to its advantages such as convenient operation and high efficiency. Its core is to separate the target cells labeled with magnetic beads from other components through the action of magnetic field. The subsequent magnetic bead recovery effect directly affects the sorting accuracy and experimental cost.

[0003] Existing magnetic bead recycling devices mostly use a fixed permanent magnetic field structure, the magnetic field strength is not adjustable, it is difficult to adapt to magnetic beads of different magnetic specifications, and the flexibility of use is insufficient.

[0004] Meanwhile, although some devices achieve magnetic field regulation, the electromagnetic coils easily generate a lot of heat during operation. They lack efficient heat conduction and heat dissipation structures, and high temperatures can easily lead to coil performance degradation and shorten service life.

[0005] Therefore, there is an urgent need for a high-throughput cell sorting magnetic bead recovery device that has an adjustable magnetic field, good heat dissipation, excellent shielding effect, and is easy to maintain. Utility Model Content

[0006] The purpose of this invention is to provide a high-throughput cell sorting magnetic bead recovery device to solve the problems mentioned in the background art.

[0007] To achieve the above objectives, this utility model provides the following technical solution: a high-throughput cell sorting magnetic bead recovery device, comprising a placement base, wherein the top of the placement base is provided with an installation groove facing downwards, and a storage tube is installed in the installation groove. The storage tube can be made of non-ferromagnetic material (including but not limited to copper and aluminum) or ferromagnetic material (such as pure iron) according to the magnetic field requirements, to adapt to the control requirements of different magnetic field strengths. The storage tube has a storage cavity inside, which is used to store cell sorting test tubes. An electromagnetic coil is provided on the outside of the storage tube. When the electromagnetic coil is energized, a reaction magnetic field is formed inside the storage cavity. A shielding cover is provided on the outside of the electromagnetic coil. An internal heat-conducting structure is provided between the shielding cover and the electromagnetic coil. An external heat-conducting structure is provided on the outside of the shielding cover. A limiting edge is provided at the top of the shielding cover. An installation edge is provided at the top of the storage tube. The installation edge is detachably engaged in the installation groove and located above the limiting edge.

[0008] The present invention is further configured such that the internal thermally conductive structure includes a thermally conductive insulating pad layer, the thermally conductive insulating pad layer being made of, but not limited to, polyimide film, silicone glass cloth or soft mica sheet, etc., and a thermally conductive silicone sheet is disposed between the thermally conductive insulating pad layer and the shielding cover, the thermally conductive silicone preferably being insulating thermally conductive silicone, and secondly being conventional thermally conductive silicone.

[0009] The present invention is further configured such that the shielding cover includes a heat-conducting cover body, which can be made of materials with good thermal conductivity such as copper, copper, or aluminum. The heat-conducting cover body has a shielding layer inside, which is made of thin permalloy to absorb and guide low-frequency magnetic fields.

[0010] The present invention is further configured such that the external heat-conducting structure includes a heat-conducting sleeve, the inner side of which is fitted to the outer wall of the shielding cover, and a heat-conducting plate is provided on the outer side of the heat-conducting sleeve. The heat-conducting plate extends to the outer side of the placement base and forms heat dissipation fins. The heat-conducting sleeve can be integrally set with the heat-conducting cover or separately set. If it is set separately, the shielding cover can be easily disassembled. If it is set as an integral unit, the overall heat dissipation effect is better, ensuring the performance and service life of the electromagnetic coil. The heat-conducting sleeve, the heat-conducting plate, and the heat dissipation fins are preferably made of materials with good thermal conductivity and heat dissipation performance, such as copper, copper, or aluminum.

[0011] The present invention is further configured such that a control component is provided on the placement base. The control component is used to control the operation of the electromagnetic coil. The control component may include a control panel and a control element group. The control element group includes a controller (microcontroller, single-chip microcomputer, etc.), a temperature controller, and other related electrical components. The specific operating principle has been disclosed in Chinese patent document CN220246135U, and the present invention will not elaborate on it. The selection of the control component in the present invention includes, but is not limited to, the above-mentioned control structure. All combinations of electrical components that can realize the operation function of the electromagnetic coil of the present invention can be used in the present invention. Here, the control panel includes, but is not limited to, traditional control button type control panel and existing touch screen type control panel. The cable connection between related electrical components can be realized by existing technology, and the present invention does not limit it.

[0012] The present invention is further configured such that a battery pack is provided inside the placement base, and a charging port is provided in conjunction with the battery pack. The battery pack is used to power the control components and the electromagnetic coil. Corresponding electrical components such as a settable voltage converter, inverter, and overload protection components (freewheeling diode, RC absorption circuit) are provided in conjunction with it. The selection and operation of the above structure can be realized by existing technology and can be selected according to actual needs. The present invention does not limit this.

[0013] The present invention is further provided with an anti-slip pad on the surface of the placement seat, so that it can be more stable when the operator holds the entire placement seat to pour the cell liquid in the test tube outward.

[0014] The present invention is further provided with an anti-slip gasket at the top of the inner wall of the storage cavity, which can enhance the stability of the cell sorting test tube when placed in the storage cavity and prevent the test tube from accidentally falling off when tilted outward.

[0015] Compared with the prior art, the beneficial effects of this utility model are: 1. This device uses an electromagnetic coil to achieve adjustable magnetic field strength, adapting to the sorting needs of different magnetic beads. Operators can set preset programs through the control components to adjust the magnetic field strength generated by the electromagnetic coil, so that the magnetic beads in the cell sorting tube are accurately adsorbed onto the tube wall, achieving efficient separation from impurity liquids, significantly improving sorting adaptability and flexibility, and meeting the needs of diverse experimental scenarios.

[0016] 2. The storage tube adopts a detachable snap-fit ​​design, making installation and replacement convenient and efficient. The mounting edge at the top of the storage tube can be detachably snapped into the mounting groove of the placement base, and the limiting edge of the shielding cover can limit and fix it. When the storage tube is damaged, aged, or needs to be replaced with a different material to adapt to the magnetic field due to long-term use, it can be quickly disassembled and replaced. Mature methods such as interference fit or toothed limiting are used to ensure installation stability while reducing maintenance costs.

[0017] 3. The shielding cover and the inner and outer heat-conducting structures work together to ensure both magnetic field stability and heat dissipation. The shielding layer on the inner side of the shielding cover reduces external interference with the magnetic field and improves separation accuracy. The thermally conductive insulating pad and thermally conductive silicone pad of the inner heat-conducting structure, and the thermally conductive sleeve and heat dissipation fins of the outer heat-conducting structure form an efficient heat conduction path to promptly dissipate the heat generated by the electromagnetic coil, preventing high temperatures from affecting coil performance and lifespan, thus achieving dual protection of shielding and heat dissipation. Attached Figure Description

[0018] Figure 1 This is a schematic diagram of the overall structure of a high-throughput cell sorting magnetic bead recovery device according to the present invention. Figure 1 ; Figure 2 This is a cross-sectional view of the overall structure of the present invention. Figure 1 ; Figure 3 This is a cross-sectional view of the overall structure of the present invention. Figure 2 ; Figure 4 This is an exploded view of the connection structure between the heat-conducting sleeve and the shielding cover in this utility model; Figure 5 This is a cross-sectional schematic diagram of the partial mating structure between the heat-conducting cover and the shielding layer in this utility model; Figure 6 This is a schematic diagram of a partial mating structure between the thermally conductive insulating pad layer and the thermally conductive silicone sheet in this utility model.

[0019] The components represented by each number in the attached diagram are listed below: 1. Placement base; 2. Mounting slot; 3. Storage tube; 4. Storage cavity; 5. Electromagnetic coil; 6. Shielding cover; 7. Limiting edge; 8. Mounting edge; 9. Thermally conductive insulating pad layer; 10. Thermally conductive silicone sheet; 11. Thermally conductive cover; 12. Shielding layer; 13. Thermally conductive sleeve; 14. Thermally conductive sheet; 15. Heat dissipation fins; 16. Battery pack; 17. Anti-slip pad; 18. Anti-slip washer. Detailed Implementation

[0020] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0021] This utility model provides a technical solution: Please refer to Figures 1-6 A high-throughput cell sorting magnetic bead recovery device includes a placement base 1, with a downward-facing mounting groove 2 on the top of the placement base 1. A storage tube 3 is installed in the mounting groove 2. The storage tube 3 can be made of materials including, but not limited to, iron, copper, and aluminum, depending on the magnetic field requirements. The storage tube 3 has a storage cavity 4 inside, which is used to store cell sorting test tubes. An electromagnetic coil 5 is provided on the outside of the storage tube 3. The electromagnetic coil 5 can be made of 0.1-0.5mm enameled copper wire. When the electromagnetic coil 5 is energized, a reaction magnetic field is formed inside the storage cavity 4. A shielding cover 6 is provided on the outside of the electromagnetic coil 5. An internal heat-conducting structure is provided between the shielding cover 6 and the electromagnetic coil 5. An external heat-conducting structure is provided on the outside of the shielding cover 6. A limiting edge 7 is provided at the top of the shielding cover 6. An installation edge 8 is provided at the top of the storage tube 3. The installation edge 8 is detachably engaged in the mounting groove 2 and located above the limiting edge 7.

[0022] Please see Figures 1-6 As one embodiment of the present invention: the internal heat-conducting structure of the present invention includes a heat-conducting insulating pad layer 9. The heat-conducting insulating pad layer 9 is made of, but is not limited to, polyimide film, silicone glass cloth or soft mica sheet. A heat-conducting silicone sheet 10 is provided between the heat-conducting insulating pad layer 9 and the shielding cover 6. The heat-conducting silicone is preferably insulating heat-conducting silicone, and secondly, conventional heat-conducting silicone.

[0023] Please see Figures 1-6 As one embodiment of the present invention: the shielding cover 6 of the present invention includes a heat-conducting cover 11, which can be made of materials with good thermal conductivity such as copper, copper, or aluminum. The heat-conducting cover 11 has a shielding layer 12 inside, which uses a thin permalloy to absorb and guide low-frequency magnetic fields.

[0024] Please see Figures 1-6 As one embodiment of this utility model: the external heat-conducting structure of this utility model includes a heat-conducting sleeve 13. The inner side of the heat-conducting sleeve 13 is attached to the outer wall of the shielding cover 6. A heat-conducting plate 14 is provided on the outer side of the heat-conducting sleeve 13. The heat-conducting plate 14 extends to the outer side of the placement base 1 and forms a heat dissipation fin 15. The heat-conducting sleeve 13 can be integrally set with the heat-conducting cover 11 or separately set. If it is set separately, the shielding cover 6 can be easily disassembled. If it is set as an integral unit, the overall heat dissipation effect is better, ensuring the use effect and service life of the electromagnetic coil 5. The heat-conducting sleeve 13, the heat-conducting plate 14 and the heat dissipation fin 15 are preferably made of materials with good thermal conductivity and heat dissipation performance, such as copper, copper, and aluminum.

[0025] Please see Figures 1-6 As one embodiment of this utility model: the placement base 1 of this utility model is provided with a control component, which is used to control the operation of the electromagnetic coil 5. The control component may include a control panel and a control element group. The control element group includes a controller (microcontroller, single-chip microcomputer, etc.), a temperature controller, and other related electrical components. The specific operating principle has been disclosed in Chinese patent document with publication number CN220246135U, and this utility model will not repeat it. The selection of the control component in this utility model includes, but is not limited to, the above-mentioned control structure. All combinations of electrical components that can realize the operation function of the electromagnetic coil 5 of this utility model can be used in this utility model. Here, the control panel includes, but is not limited to, traditional control button type control panel and existing touch screen type control panel. The cable connection between related electrical components can be realized by existing technology, and this utility model does not limit it.

[0026] Please see Figures 1-6 As one embodiment of this utility model: the placement base 1 of this utility model is provided with a battery pack 16 inside, and a charging port is provided in conjunction with the battery pack 16. The battery pack 16 is used to supply power to the control components and the electromagnetic coil 5. The corresponding electrical components such as the adjustable voltage converter, inverter, and overload protection components (freewheeling diode, RC absorption circuit) are also provided. The selection and operation of the above structures can be realized by existing technology and can be selected according to actual needs. This utility model does not limit this.

[0027] Please see Figures 1-6 As one embodiment of the present invention: the surface of the placement seat 1 is provided with an anti-slip pad 17, so that when the operator holds the placement seat 1 as a whole to pour the cell liquid in the test tube outward, it can be more stable.

[0028] Please see Figures 1-6As one embodiment of the present invention: the top of the inner wall of the storage cavity 4 is provided with an anti-slip gasket 18, which can enhance the stability of the cell sorting test tube when placed in the storage cavity 4 and prevent the test tube from accidentally falling off when tilted outward.

[0029] In summary: This invention, by employing an electromagnetic coil 5, can adjust the magnitude of the magnetic field through a preset program controlled by electricity. This allows it to adapt to different magnetic bead magnetic strengths during cell sorting, enabling the magnetic beads in the cell sorting test tube to adhere to the inner wall of the test tube and separate from other impurity liquids, thus achieving cell sorting and separation from the magnetic beads. In this utility model, the storage tube 3 for placing and securing test tubes can be detachably snapped into the mounting groove 2. In this way, when the storage tube 3 is damaged or aged after long-term use, it can be flexibly disassembled and replaced. Here, the snapping installation of the storage tube 3 can adopt the interference snapping method in the prior art, or the toothed limiting snapping method in the prior art. The above methods are all prior art, and this utility model will not elaborate on them. In this invention, a shielding cover 6 is provided on the outside of the electromagnetic coil 5. This reduces the influence of external factors on the magnetic field formed by the electromagnetic coil 5 and improves the separation effect between the extract and the magnetic beads. The shielding cover 6 is provided with an inner heat-conducting structure and an outer heat-conducting structure, respectively, to ensure the electromagnetic shielding effect while ensuring the heat dissipation effect of the electromagnetic coil 5 during operation.

[0030] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0031] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A high-throughput cell sorting magnetic bead recovery device, comprising a placement base (1), characterized in that: The top of the placement seat (1) is provided with an installation groove (2) facing downwards. A storage tube (3) is installed in the installation groove (2). The storage tube (3) has a storage cavity (4) inside. The storage cavity (4) is used to store cell sorting test tubes. An electromagnetic coil (5) is provided on the outside of the storage tube (3). A shield (6) is provided on the outside of the electromagnetic coil (5). An internal heat conduction structure is provided between the shield (6) and the electromagnetic coil (5). An external heat conduction structure is provided on the outside of the shield (6). A limiting edge (7) is provided at the top of the shield (6). An installation edge (8) is provided at the top of the storage tube (3). The installation edge (8) is detachably engaged in the installation groove (2) and located above the limiting edge (7).

2. The high-throughput cell sorting magnetic bead recovery device according to claim 1, characterized in that: The internal heat-conducting structure includes a heat-conducting insulating pad layer (9), and a heat-conducting silicone sheet (10) is disposed between the heat-conducting insulating pad layer (9) and the shield (6).

3. The high-throughput cell sorting magnetic bead recovery device according to claim 2, characterized in that: The shield (6) includes a heat-conducting cover (11), and a shielding layer (12) is provided inside the heat-conducting cover (11).

4. The high-throughput cell sorting magnetic bead recovery device according to claim 3, characterized in that: The external heat-conducting structure includes a heat-conducting sleeve (13), the inner side of which is attached to the outer wall of the shield (6), and a heat-conducting plate (14) is provided on the outer side of the heat-conducting sleeve (13). The heat-conducting plate (14) extends to the outer side of the placement seat (1) and forms heat dissipation fins (15).

5. The high-throughput cell sorting magnetic bead recovery device according to claim 1, characterized in that: The placement base (1) is provided with a control component, which is used to control the operation of the electromagnetic coil (5).

6. A high-throughput cell sorting magnetic bead recovery device according to claim 5, characterized in that: The placement base (1) is equipped with a battery pack (16) and a charging port is provided in conjunction with the battery pack (16). The battery pack (16) is used to supply power to the control components and the electromagnetic coil (5).

7. The high-throughput cell sorting magnetic bead recovery device according to claim 1, characterized in that: The surface of the placement seat (1) is provided with anti-slip pads (17).

8. The high-throughput cell sorting magnetic bead recovery device according to claim 1, characterized in that: The top of the inner wall of the storage cavity (4) is provided with an anti-slip gasket (18).

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