Magnetic cell scraping cell culture flask
Patent Information
- Application Number
- CN202522369023.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-11-07
- Publication Date
- 2026-10-23
- Estimated Expiration
- 2035-11-07
AI Technical Summary
[0003]为了克服上述缺陷，本实用新型提供了一种磁吸式细胞刮取细胞培养瓶，解决了一些细胞在培养过程中需要氧气，现有细胞培养瓶一般会进行开孔来满足氧气的供给，但是由于不同类型的细胞所需要的氧气有所偏差，固定数量的孔能够提供的氧气是确定的，难以满足不同氧气需求的细胞，并且细胞在培养过程中一部分细胞会粘黏在细胞培养瓶的内底部，在后续对细胞悬液进行收集的过程中，容易出现收集不完全的情况的问题
1、该磁吸式细胞刮取细胞培养瓶，通过设置金属刮条、磁铁、筒体、透气孔、限位孔、橡胶塞、弹簧与活动杆，使用时，拉动圆帽带动活动杆与圆板向上移动，圆板会将弹簧进行压缩，且活动杆会脱离其中一个限位孔的内部从而解除活动柱的固定状态，此时即可拉动拉板带动橡胶塞进行移动，从而根据细胞的需氧量来调节接通的透气孔的数量，当调节完毕后，将圆帽松开使得弹簧复位并推动圆板与活动杆向下移动，使得活动杆插接至其中一个限位孔的内部来对活动柱与橡胶塞的位置进行固定即可，然后向细胞培养瓶主体内部添加营养液与细胞进行培养，当需要将粘黏在细胞培养瓶主体内底部的细胞刮下时，拉动柱体带动连接杆与磁铁进行移动，磁铁会将金属刮条进行吸引从而带动金属刮条移动并将粘黏在细胞培养瓶主体内底部的细胞刮下，使得细胞重新悬置于营养液中，该培养瓶能够通过调节接通的透气孔的数量来对氧气的供给量进行调节，能够满足不同氧气需求的细胞，且通过磁铁吸附金属刮条进行移动来将细胞刮下并使其重新悬置于营养液中，避免后续出现收集不完全的情况，且无需将细胞培养瓶主体的瓶盖打开即可进行操作，降低了污染风险；
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Figure CN224884076U_ABST
Abstract
Description
Technical Field
[0001] This invention belongs to the field of cell culture flask technology, specifically a magnetic cell scraping cell culture flask. Background Technology
[0002] Cell culture flasks are specialized containers used for cell culture. They are typically made of transparent plastic or glass, possess good biocompatibility, and provide a suitable growth environment for cells. Some cells require oxygen during culture, and existing cell culture flasks generally have openings to meet this oxygen supply. However, since different types of cells have varying oxygen requirements, a fixed number of openings can only provide a limited amount of oxygen, making it difficult to meet the diverse oxygen needs of different cells. Furthermore, during culture, some cells adhere to the bottom of the cell culture flask, leading to incomplete collection of the cell suspension later on. Utility Model Content
[0003] To overcome the above-mentioned defects, this utility model provides a magnetic cell scraping cell culture flask, which solves the problem that some cells require oxygen during the culture process. Existing cell culture flasks generally have holes to meet the oxygen supply. However, since different types of cells have different oxygen requirements, the oxygen that can be provided by a fixed number of holes is limited, which is difficult to meet the needs of cells with different oxygen requirements. In addition, during the culture process, some cells will stick to the bottom of the cell culture flask, which can easily lead to incomplete collection during the subsequent collection of cell suspension.
[0004] To achieve the above objectives, this utility model provides the following technical solution: a magnetic cell scraping cell culture flask, comprising a cell culture flask body, a metal scraper provided inside the cell culture flask body, sliders fixedly connected to both ends of the metal scraper, a magnet provided at the bottom of the cell culture flask body, the magnet being magnetically connected to the metal scraper, a cap threadedly connected to one side of the cell culture flask body, a cylinder fixedly connected to one side of the cap, a plurality of vent holes provided on the cylinder, a sterile filter membrane fixedly connected inside the vent holes, and a connecting plate fixedly connected to the cylinder; A fixed plate is fixedly connected to the connecting plate, a movable rod is slidably connected through the fixed plate, a circular plate is fixedly connected to the movable rod, a spring is sleeved on the movable rod, a movable column is slidably connected through the connecting plate, a plurality of limiting holes are opened on the movable column, a movable plate is fixedly connected to one end of the movable column, and a rubber plug is fixedly connected to the movable plate.
[0005] As a further embodiment of this utility model: one end of the spring is fixedly connected to the circular plate, the other end of the spring is fixedly connected to the fixed plate, a round cap is fixedly connected to the top of the movable rod, and a pull plate is fixedly connected to one end of the movable column.
[0006] As a further embodiment of this utility model: a silicone sleeve is provided on the outside of the metal scraper, and sliders are fixedly connected to both ends of the metal scraper. A groove is provided inside the cell culture flask body corresponding to the position of the slider, and the slider is slidably connected inside the groove.
[0007] As a further embodiment of this utility model: two L-shaped supports are fixedly connected to the bottom of the cell culture flask body, several suction cups are fixedly connected to the bottom of the L-shaped supports, several connecting strips are fixedly connected to the L-shaped supports, a support plate is provided at the bottom of the cell culture flask body, and the support plate is fixed to the connecting strips.
[0008] As a further embodiment of this utility model: a connecting block is provided on the top of the support plate, the connecting block is fixedly connected to the bottom of the magnet, a connecting rod is fixedly connected to the connecting block, and a column is fixedly connected to one end of the connecting rod.
[0009] As a further embodiment of this utility model: a T-shaped strip is fixedly connected to the bottom of the connecting block, and a T-shaped groove is provided on the support plate at the position corresponding to the T-shaped strip, with the T-shaped strip located inside the T-shaped groove.
[0010] As a further embodiment of this utility model: two fixing strips are fixedly connected to the movable column, and a square hole is opened on the connecting plate corresponding to the position of the fixing strip, and a round hole is opened on the connecting plate corresponding to the position of the movable column.
[0011] Compared with the prior art, the beneficial effects of this utility model are as follows: 1. This magnetic cell scraper culture flask is equipped with a metal scraper, magnet, cylinder, vent, limiting hole, rubber stopper, spring, and movable rod. In use, pulling the cap moves the movable rod and circular plate upwards. The circular plate compresses the spring, and the movable rod disengages from one of the limiting holes, releasing the fixed position of the movable column. At this point, pulling the pull plate moves the rubber stopper, adjusting the number of vents opened according to the cell's oxygen demand. After adjustment, releasing the cap resets the spring and pushes the circular plate and movable rod downwards, allowing the movable rod to engage with one of the limiting holes to fix the position of the movable column and rubber stopper. Then, fill the cell culture flask with... The cell culture flask is culturing cells with nutrient solution. When it is necessary to scrape off the cells adhering to the bottom of the cell culture flask, the column is pulled to move the connecting rod and magnet. The magnet attracts the metal scraper, which moves and scrapes off the cells adhering to the bottom of the cell culture flask, allowing the cells to be resuspended in the nutrient solution. The oxygen supply of this culture flask can be adjusted by changing the number of vents to meet the oxygen requirements of cells with different needs. The use of a magnet to attract and move the metal scraper to scrape off the cells and resuspend them in the nutrient solution avoids incomplete collection later. The operation can be performed without opening the cap of the cell culture flask, reducing the risk of contamination. 2. This magnetic cell scraper for cell culture flasks is equipped with a sliding groove, a slider, a T-shaped strip, and a T-groove. When the connecting block moves, the T-shaped strip slides inside the T-groove. The T-groove serves to limit and guide the T-shaped strip, the connecting block, and the magnet, improving the stability and accuracy of the magnet's movement. When the metal scraper moves, the slider slides inside the sliding groove, which also limits and guides the metal scraper, improving its stability and accuracy. Attached Figure Description
[0012] Figure 1 This is a three-dimensional structural diagram of the present invention; Figure 2 This is a three-dimensional structural diagram of the main body of the cell culture flask of this utility model; Figure 3 This is a schematic diagram of the structure of the present invention, showing the separation of the T-shaped strip and the T-shaped groove; Figure 4 This is a schematic diagram of the cross-sectional structure of the cell culture flask of this utility model; Figure 5 This is a structural schematic diagram of the cross-section of the silicone sleeve of this utility model; Figure 6 This is a structural schematic diagram of the cross-section of the cylinder and connecting plate of this utility model; Figure 7 This utility model Figure 6Enlarged structural diagram at point A; Figure 8 This is a three-dimensional structural diagram of the connecting plate of this utility model; In the diagram: 1. Cell culture flask body; 2. L-shaped support; 3. Cylinder; 4. Connecting strip; 5. Support plate; 6. T-groove; 7. T-strip; 8. Connecting block; 9. Magnet; 10. Connecting rod; 11. Column; 12. Slide groove; 13. Silicone sleeve; 14. Metal scraper; 15. Sliding block; 16. Connecting plate; 17. Movable plate; 18. Rubber stopper; 19. Movable column; 20. Fixing strip; 21. Limiting hole; 22. Vent hole; 23. Pull plate; 24. Fixing plate; 25. Movable rod; 26. Round cap; 27. Round plate; 28. Spring; 29. Round hole; 30. Square hole; 31. Bottle cap; 32. Suction cup; 33. Sterile filter membrane. Detailed Implementation
[0013] The technical solution of this patent will be further described in detail below with reference to specific embodiments.
[0014] like Figure 1-8 As shown, this utility model provides a technical solution: a magnetic cell scraping cell culture flask, including a cell culture flask body 1, a metal scraper 14 inside the cell culture flask body 1, sliders 15 fixedly connected to both ends of the metal scraper 14, a magnet 9 at the bottom of the cell culture flask body 1, the magnet 9 being magnetically connected to the metal scraper 14, a bottle cap 31 threadedly connected to one side of the cell culture flask body 1, a cylinder 3 fixedly connected to one side of the bottle cap 31, a spring 28 fixedly connected to one end of a circular plate 27, a fixed plate 24 at the other end of the spring 28, a round cap 26 fixedly connected to the top of the movable rod 25, and a pull plate 23 fixedly connected to one end of the movable column 19.
[0015] The cylinder 3 has several vent holes 22, and a sterile filter membrane 33 is fixedly connected inside the vent holes 22. Because the sterile filter membrane 33 has a microporous structure, it allows gas to pass through but prevents liquid and microorganisms from penetrating, ensuring air permeability while preventing the cells inside the cell culture flask body 1 from being contaminated. A connecting plate 16 is fixedly connected to the cylinder 3, and a fixing plate 24 is fixedly connected to the connecting plate 16. A movable rod 25 is slidably connected through the fixing plate 24. Because the movable rod 25 is provided, it can limit the spring 28. When the spring 28 is compressed, the movable rod 25 can prevent the spring 28 from bending excessively and deforming, ensuring the normal operation of the spring 28. A circular plate 27 is fixedly connected to the movable rod 25. A silicone sleeve 13 is provided on the outside of the metal scraper 14. A slider 15 is fixedly connected to both ends of the metal scraper 14. A groove 12 is opened inside the cell culture flask body 1 at the position corresponding to the slider 15. The slider 15 is slidably connected inside the groove 12.
[0016] A spring 28 is sleeved on the movable rod 25. A movable column 19 is slidably connected through the connecting plate 16. Several limiting holes 21 are opened on the movable column 19. A movable plate 17 is fixedly connected to one end of the movable column 19. A rubber stopper 18 is fixedly connected to the movable plate 17. Because of the rubber stopper 18, the rubber stopper 18 can seal the cylinder 3 and prevent oxygen from entering through the unconnected vent hole 22, thereby ensuring that the oxygen supply adjustment can be carried out normally. Two L-shaped supports 2 are fixedly connected to the bottom of the cell culture bottle body 1. Several suction cups 32 are fixedly connected to the bottom of the L-shaped supports 2. Because of the suction cups 32, the cell culture bottle body 1 is adsorbed on the table by the suction cups 32, which can effectively improve the stability of the cell culture bottle body 1 and prevent the cell culture bottle body 1 from moving during operation, thus facilitating personnel operation. Several connecting strips 4 are fixedly connected to the L-shaped supports 2. A support plate 5 is set at the bottom of the cell culture bottle body 1, and the support plate 5 is fixed to the connecting strips 4.
[0017] A connecting block 8 is provided on the top of the support plate 5. The connecting block 8 is fixedly connected to the bottom of the magnet 9. A connecting rod 10 is fixedly connected to the connecting block 8. A column 11 is fixedly connected to one end of the connecting rod 10. A T-shaped strip 7 is fixedly connected to the bottom of the connecting block 8. A T-shaped groove 6 is provided on the support plate 5 at the position corresponding to the T-shaped strip 7. Because of the T-shaped strip 7 and the T-shaped groove 6, the T-shaped strip 7 will slide inside the T-shaped groove 6 when the connecting block 8 moves. The T-shaped groove 6 can limit and guide the T-shaped strip 7, the connecting block 8 and the magnet 9. The T-shaped strip 7 is located inside the T-shaped groove 6. Two fixing strips 20 are fixedly connected to the movable column 19. A square hole 30 is provided on the connecting plate 16 at the position corresponding to the fixing strip 20. Because of the square hole 30 and the fixing strip 20, the square hole 30 can limit the fixing strip 20 and prevent the movable column 19 from rotating. A round hole 29 is provided on the connecting plate 16 at the position corresponding to the movable column 19.
[0018] The working principle of this utility model is as follows: In use, pulling the round cap 26 moves the movable rod 25 and the round plate 27 upwards. The round plate 27 compresses the spring 28, and the movable rod 25 disengages from one of the limiting holes 21, thus releasing the fixed state of the movable column 19. At this time, the pull plate 23 can be pulled to move the rubber stopper 18, thereby adjusting the number of connected vent holes 22 according to the oxygen demand of the cells. After adjustment, the round cap 26 is released, causing the spring 28 to return to its original position and pushing the round plate 27 and the movable rod 25 downwards, allowing the movable rod 25 to insert... The movable column 19 and the rubber stopper 18 can be fixed by connecting to the inside of one of the limiting holes 21. Then, nutrient solution and cells are added into the cell culture flask body 1 for culture. When it is necessary to scrape off the cells stuck to the bottom of the cell culture flask body 1, the column 11 is pulled to move the connecting rod 10 and the magnet 9. The magnet 9 will attract the metal scraper 14, thereby moving the metal scraper 14 and scraping off the cells stuck to the bottom of the cell culture flask body 1, so that the cells are resuspended in the nutrient solution.
[0019] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.
[0020] The preferred embodiments of this patent have been described in detail above. However, this patent is not limited to the above embodiments. Within the scope of knowledge possessed by those skilled in the art, various changes can be made without departing from the spirit of this patent.
Claims
1. A magnetic cell scraping culture flask, comprising a cell culture flask body (1), characterized in that: The cell culture flask body (1) is provided with a metal scraper (14) inside. Both ends of the metal scraper (14) are fixedly connected to sliders (15). The cell culture flask body (1) is provided with a magnet (9) at the bottom. The magnet (9) is magnetically connected to the metal scraper (14). A bottle cap (31) is threadedly connected to one side of the cell culture flask body (1). A cylinder (3) is fixed and connected to one side of the bottle cap (31). Several vent holes (22) are opened on the cylinder (3). A sterile filter membrane (33) is fixedly connected inside the vent holes (22). A connecting plate (16) is fixedly connected to the cylinder (3). A fixed plate (24) is fixedly connected to the connecting plate (16). A movable rod (25) is slidably connected through the fixed plate (24). A circular plate (27) is fixedly connected to the movable rod (25). A spring (28) is sleeved on the movable rod (25). A movable column (19) is slidably connected through the connecting plate (16). Several limiting holes (21) are opened on the movable column (19). A movable plate (17) is fixedly connected to one end of the movable column (19). A rubber plug (18) is fixedly connected to the movable plate (17).
2. The magnetic cell scraping culture flask according to claim 1, characterized in that: One end of the spring (28) is fixedly connected to the circular plate (27), and the other end of the spring (28) is fixedly connected to the fixed plate (24). A round cap (26) is fixedly connected to the top of the movable rod (25), and a pull plate (23) is fixedly connected to one end of the movable column (19).
3. The magnetic cell scraping culture flask according to claim 1, characterized in that: The metal scraper (14) is provided with a silicone sleeve (13) on the outside. Both ends of the metal scraper (14) are fixedly connected to sliders (15). The cell culture flask body (1) is provided with a groove (12) corresponding to the position of the slider (15). The slider (15) is slidably connected to the inside of the groove (12).
4. The magnetic cell scraping culture flask according to claim 1, characterized in that: The cell culture flask body (1) has two L-shaped supports (2) fixedly connected to the bottom. The L-shaped supports (2) have several suction cups (32) fixedly connected to the bottom. The L-shaped supports (2) have several connecting strips (4) fixedly connected to the top. The cell culture flask body (1) has a support plate (5) at the bottom. The support plate (5) is fixed to the connecting strips (4).
5. A magnetic cell scraping culture flask according to claim 4, characterized in that: The support plate (5) is provided with a connecting block (8) at the top. The connecting block (8) is fixedly connected to the bottom of the magnet (9). A connecting rod (10) is fixedly connected to the connecting block (8). A column (11) is fixedly connected to one end of the connecting rod (10).
6. A magnetic cell scraping culture flask according to claim 5, characterized in that: The bottom of the connecting block (8) is fixedly connected to a T-shaped strip (7), and a T-shaped groove (6) is provided on the support plate (5) at the position corresponding to the T-shaped strip (7), and the T-shaped strip (7) is located inside the T-shaped groove (6).
7. A magnetic cell scraping culture flask according to claim 1, characterized in that: Two fixing strips (20) are fixedly connected to the movable column (19). A square hole (30) is opened on the connecting plate (16) corresponding to the fixing strip (20), and a round hole (29) is opened on the connecting plate (16) corresponding to the movable column (19).