A shock device for expanding sub-totipotent stem cells

Abstract

The utility model discloses a kind of sub-pancreas stem cell amplification with shock equipment, belong to the technical field of shock device, by the first rotating seat and second rotating seat are connected by the insertion of insertion tube and insertion column, form the double-layer structure of upper and lower superposition, each layer is equipped with multiple accommodating holes, can simultaneously accommodate multiple groups of container, while can adapt to the container demand of different height.This sub-pancreas stem cell amplification with shock equipment includes fixed base, first rotating seat is rotatably embedded and installed on the fixed base, second rotating seat is installed above first rotating seat, insertion tube is vertically fixed at the center of first rotating seat and second rotating seat, insertion column is fixed at the center of second rotating seat bottom, insertion column on second rotating seat is inserted and installed at insertion tube at the top of first rotating seat, accommodating hole for accommodating container is formed on first rotating seat and second rotating seat;Protruding seat is arranged on the outside of fixed base, and electric telescopic rod is installed on protruding seat.

Description

Technical Field

[0001] This utility model belongs to the field of vibration device technology, specifically relating to a vibration device for the expansion of sub-pluripotent stem cells. Background Technology

[0002] Stem cells are a type of pluripotent cell with the ability to self-renew. Under certain conditions, they can differentiate into various functional cells. Based on their developmental stage, stem cells are classified into embryonic stem cells and adult stem cells. Based on their developmental potential, they are classified into three categories: totipotent stem cells, multipotent stem cells, and unipotent stem cells. Stem cell expansion is the process of isolating and purifying autologous, allogeneic, or xenogeneic stem cells, culturing them under sterile conditions to increase their number.

[0003] Existing shaking devices have significant shortcomings in terms of container capacity and compatibility: on the one hand, most devices can only hold a single layer of containers, resulting in limited processing capacity per cycle, which is insufficient to meet the needs of large-scale expansion; on the other hand, the container height is fixed, and changing to different sizes (such as different heights) of culture containers requires adjusting the device structure or replacing parts, which is cumbersome and inefficient. Therefore, a shaking device for sub-pluripotent stem cell expansion is needed to help solve this problem. Utility Model Content

[0004] (1) Technical problems to be solved

[0005] To address the shortcomings of existing technologies, the purpose of this invention is to provide a vibration device for expanding sub-pluripotent stem cells. This device forms a double-layered structure by connecting a first rotating seat and a second rotating seat through a plug-in connection between a plug-in cylinder and a plug-in column. Each layer has multiple receiving holes, which can accommodate multiple sets of containers simultaneously. The second rotating seat can adapt to the needs of containers of different heights by adjusting the insertion depth of the plug-in column in the plug-in cylinder (with locking bolts), significantly improving the device's compatibility with containers of various sizes and its operational efficiency.

[0006] (2) Technical solution

[0007] To solve the above-mentioned technical problems, this utility model provides a vibration device for expanding sub-pluripotent stem cells. The vibration device for expanding sub-pluripotent stem cells includes a fixed base, on which a first rotating base is rotatably embedded. A second rotating base is installed above the first rotating base. A plug-in cylinder is vertically fixed at the center of the first rotating base and the second rotating base. A plug-in post is fixed at the center of the bottom of the second rotating base. The plug-in post on the second rotating base is plugged into the plug-in cylinder at the top of the first rotating base. Both the first rotating base and the second rotating base are provided with receiving holes for accommodating containers.

[0008] A protruding seat is provided on the outer side of the fixed seat, and an electric telescopic rod that assists the first rotating seat to reciprocate is installed on the protruding seat.

[0009] Furthermore, the first rotating seat and the second rotating seat are provided with clamping cavities at the outer edges of the receiving hole, and clamping components are installed in the clamping cavities.

[0010] Furthermore, the clamping assembly includes a sliding rod slidably disposed within the clamping cavity, one end of the sliding rod being fixed with a pressure plate, and an ejector spring fitted onto the sliding rod to assist the pressure plate in pressing against the sliding rod.

[0011] Furthermore, the clamping plate has an arc-shaped plate structure, and an anti-slip pad is embedded on the inner side of the clamping plate.

[0012] Furthermore, a fixing bolt is threadedly installed on the outer edge of the plug-in tube, and the plug-in tube and the fixing bolt are threadedly engaged.

[0013] Furthermore, the outer edge of the fixing base is provided with a fixing ear, and the fixing ear is provided with a fixing hole.

[0014] Furthermore, the electric telescopic rod is hinged to the protruding seat, and the extended end of the electric telescopic rod is hinged to the outer edge of the first rotating seat.

[0015] (3) Beneficial effects

[0016] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0017] This invention connects the first rotating seat and the second rotating seat through a plug-in tube and a plug-in post to form a double-layer structure with each layer having multiple receiving holes, which can accommodate multiple sets of containers at the same time. The second rotating seat can adapt to the needs of containers of different heights by adjusting the insertion depth of the plug-in post in the plug-in tube (with the help of fixing bolts to lock it), which significantly improves the equipment's compatibility with containers of various sizes and its operating efficiency.

[0018] This invention utilizes a clamping assembly, in which an ejector spring, a sliding rod, and a pressure plate work together to adapt to containers of different diameters. The spring force of the ejector spring pushes the sliding rod towards the center of the receiving hole, causing the arc-shaped pressure plate to press against the outer wall of the container. Combined with the anti-slip pad on the inner side of the pressure plate, the curved surface fit and friction enhancement effectively prevent the container from sliding or tipping over during vibration, ensuring the safety of stem cell culture. Attached Figure Description

[0019] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0020] Figure 1 This is a schematic diagram of the structure of this utility model;

[0021] Figure 2 This is a structural diagram of the present invention during disassembly;

[0022] Figure 3 This utility model Figure 1 Enlarged structural diagram at point A;

[0023] Figure 4 This is a cross-sectional view of the fixing base of this utility model.

[0024] The labels in the attached diagram are as follows: 1. Fixed seat; 2. Fixed ear; 3. Protruding seat; 4. Electric telescopic rod; 5. First rotating seat; 6. Receiving hole; 7. Clamping assembly; 8. Sliding rod; 9. Ejection spring; 10. Pressure plate; 11. Insertion tube; 12. Insertion post; 13. Second rotating seat. Detailed Implementation

[0025] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0026] This specific embodiment is a shaking device for expanding sub-pluripotent stem cells, such as... Figure 1-4 As shown, the vibration device for expanding sub-pluripotent stem cells includes a fixed base 1, a fixed ear 2 protruding from the outer edge of the fixed base 1, a fixed hole on the fixed ear 2, a first rotating seat 5 rotatably mounted on the fixed base 1, a second rotating seat 13 mounted above the first rotating seat 5, a protruding seat 3 protruding from the outer side of the fixed base 1, an electric telescopic rod 4 for assisting the reciprocating rotation of the first rotating seat 5 mounted on the protruding seat 3, the electric telescopic rod 4 being hinged to the protruding seat 3, and the extended end of the electric telescopic rod 4 being hinged to the outer edge of the first rotating seat 5. The reciprocating rotation of the first rotating seat 5 can be achieved by the extension and retraction of the electric telescopic rod 4.

[0027] A connector 11 is vertically fixed at the center of the first rotating seat 5 and the second rotating seat 13. A connector 12 is fixed at the center of the bottom of the second rotating seat 13. The connector 12 on the second rotating seat 13 is inserted into the connector 11 at the top of the first rotating seat 5. A fixing bolt is threaded onto the outer edge of the connector 11, and the connector 11 and the fixing bolt are threaded together. Both the first rotating seat 5 and the second rotating seat 13 have receiving holes 6 for accommodating containers.

[0028] By connecting the first rotating seat 5 and the second rotating seat 13 to the plug-in tube 11 and the plug-in post 12 through a plug-in connection, a double-layer structure is formed with the upper and lower layers stacked on top of each other. Each layer is provided with multiple receiving holes 6, which can accommodate multiple sets of containers at the same time. The second rotating seat 13 can adapt to the needs of containers of different heights by adjusting the insertion depth of the plug-in post 12 in the plug-in tube 11 (with the help of fixing bolts to lock it), which significantly improves the equipment's compatibility with containers of multiple specifications and its operating efficiency.

[0029] The first rotating seat 5 and the second rotating seat 13 are provided with clamping cavities at the outer edges of the corresponding receiving holes 6. A clamping assembly 7 is installed in the clamping cavity. The clamping assembly 7 includes a sliding rod 8 that is slidably disposed in the clamping cavity. A pressure plate 10 is fixed to one end of the sliding rod 8. The pressure plate 10 has an arc-shaped plate structure. An anti-slip pad is embedded on the inner side of the pressure plate 10. An ejection spring 9 that assists the pressure plate 10 in pressing is sleeved on the sliding rod 8.

[0030] The clamping assembly 7, with its ejector spring 9, sliding rod 8, and pressure plate 10, can adapt to containers of different diameters. The elastic force of the ejector spring 9 pushes the sliding rod 8 towards the center of the receiving hole 6, causing the arc-shaped pressure plate 10 to press against the outer wall of the container. Combined with the anti-slip pad on the inner side of the pressure plate 10, the curved surface fit and friction enhancement effectively prevent the container from sliding or tipping over during vibration, ensuring the safety of stem cell culture.

[0031] Working principle:

[0032] First, the container holding the sub-pluripotent stem cells is placed in the receiving hole 6 of the first rotating seat 5 or the second rotating seat 13. At this time, the clamping component 7 in the clamping cavity pushes the sliding rod 8 towards the center of the receiving hole 6 by the elastic force of the ejector spring 9, which drives the pressure plate 10 to press against the outer wall of the container. The arc-shaped pressure plate 10 fits against the outer wall of the container, and the anti-slip pad enhances the friction to achieve stable fixation of the container. If the container is too high, the insertion depth of the insertion post 12 of the second rotating seat 13 in the insertion cylinder 11 of the first rotating seat 5 can be adjusted and locked with the fixing bolt to accommodate containers of different heights.

[0033] During oscillation, the electric telescopic rod 4 extends and retracts, its extended end pushing or pulling the first rotating seat 5 to reciprocate around the fixed seat 1. The first rotating seat 5 drives the second rotating seat 13 to rotate synchronously (achieved through the insertion of the plug-in post 12 and the plug-in tube 11). Since the second rotating seat 13 is only connected to the first rotating seat 5 through the plug-in post 12, it can undergo a slight angular displacement due to gravity or inertia during rotation, thus forming a multi-directional, multi-dimensional oscillation effect, promoting the uniform expansion of sub-pluripotent stem cells within the container. The entire device can be fixed to the support surface via the fixing lug 2 to ensure the stability of the oscillation process.

[0034] All technical features in this embodiment can be freely combined according to actual needs.

[0035] Finally, it should be noted that the above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A vibration device for expanding sub-pluripotent stem cells, the vibration device for expanding sub-pluripotent stem cells comprising a fixed base (1), characterized in that, A first rotating seat (5) is rotatably mounted on the fixed seat (1). A second rotating seat (13) is mounted above the first rotating seat (5). A plug-in tube (11) is vertically fixed at the center of the first rotating seat (5) and the second rotating seat (13). A plug-in post (12) is fixed at the center of the bottom of the second rotating seat (13). The plug-in post (12) on the second rotating seat (13) is plugged into the plug-in tube (11) at the top of the first rotating seat (5). Both the first rotating seat (5) and the second rotating seat (13) are provided with receiving holes (6) for the container to be received. A protruding seat (3) is provided on the outer side of the fixed seat (1), and an electric telescopic rod (4) is installed on the protruding seat (3) to assist the first rotating seat (5) in reciprocating rotation.

2. The oscillation device for expanding sub-pluripotent stem cells according to claim 1, characterized in that, The first rotating seat (5) and the second rotating seat (13) are provided with clamping cavities at the outer edge of the receiving hole (6), and clamping components (7) are installed in the clamping cavities.

3. The vibration device for expanding sub-pluripotent stem cells according to claim 2, characterized in that, The clamping assembly (7) includes a sliding rod (8) slidably disposed in the clamping cavity. One end of the sliding rod (8) is fixed with a pressure plate (10), and an ejection spring (9) with an auxiliary pressure plate (10) is sleeved on the sliding rod (8).

4. The oscillation device for expanding sub-pluripotent stem cells according to claim 3, characterized in that, The pressing plate (10) has an arc-shaped plate structure, and an anti-slip pad is embedded on the inner side of the pressing plate (10).

5. The oscillation device for expanding sub-pluripotent stem cells according to claim 1, characterized in that, A fixing bolt is threadedly installed on the outer edge of the plug tube (11), and the plug tube (11) and the fixing bolt are threadedly engaged.

6. The oscillation device for expanding sub-pluripotent stem cells according to claim 1, characterized in that, The outer edge of the fixing base (1) is provided with a fixing ear (2), and a fixing hole is provided on the fixing ear (2).

7. The oscillation device for expanding sub-pluripotent stem cells according to claim 1, characterized in that, The electric telescopic rod (4) is hinged to the protruding seat (3), and the extended end of the electric telescopic rod (4) is hinged to the outer edge of the first rotating seat (5).

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