Mass spectrometry sample pretreatment automated online solid phase extraction device

Abstract

The utility model relates to the field of sample pretreatment technology in analytical chemistry, concretely is mass spectrum sample pretreatment automation online solid phase extraction device, including the extraction bucket, the rotatable carousel is installed in the extraction bucket, the carousel top fixed mounting has a plurality of vertical support bars, every vertical support bar outside all sets up an extraction column. The utility model discloses a rotatable carousel is arranged in the extraction bucket, and a plurality of extraction columns are installed on the carousel with the help of vertical support bar, and the carousel is driven to rotate by the transmission shaft of rotary motor, realizes the dynamic rotation of extraction column in the extraction process. This design can make the extraction column and the sample solution in the extraction bucket fully contact, increase the contact area and contact frequency, improve the extraction efficiency of target material, replace manual operation through automatic rotation drive, improve the automation degree of pretreatment process, adapt to the requirement of mass spectrum detection to sample pretreatment efficiency and consistency.

Description

Technical Field

[0001] This utility model relates to the field of sample pretreatment technology in analytical chemistry, specifically to an automated online solid-phase extraction device for mass spectrometry sample pretreatment. Background Technology

[0002] Solid-phase extraction (SPE) is a core step in mass spectrometry sample pretreatment. It achieves the separation and enrichment of trace analytes in complex matrices through the specific adsorption-desorption interaction between the extraction column packing and the target substance, directly impacting the sensitivity, recovery rate, and data repeatability of mass spectrometry detection. However, existing SPE devices still face several challenges in meeting the demands for high efficiency and consistency in mass spectrometry detection. Insufficient extraction efficiency and mass transfer performance are common problems with traditional devices, which often employ static or unidirectional liquid flow driven modes. The contact between the extraction column and the sample solution is limited to a fixed area, resulting in a small contact area and slow mass transfer rate. This leads to insufficient adsorption of the target substance, especially when processing samples with high matrix complexity (such as serum and food extracts). The extraction cycle for a single sample is long, and uneven mass transfer increases the fluctuation range of the recovery rate, far exceeding the error range required for mass spectrometry detection. While some automated devices have achieved process automation, they have not optimized the contact method between the extraction column and the sample, and efficiency bottlenecks still exist.

[0003] In view of this, we propose an automated online solid-phase extraction device for mass spectrometry sample pretreatment. Utility Model Content

[0004] To overcome the above deficiencies, this invention provides an automated online solid-phase extraction device for mass spectrometry sample pretreatment.

[0005] The technical solution of this utility model is: An automated online solid-phase extraction (SPE) device for mass spectrometry sample pretreatment includes an extraction tank with a rotatable turntable inside. Several vertical support rods are fixedly mounted on the top of the turntable, and an extraction column is fitted onto the outer side of each support rod. A base is located below the extraction tank, and a top plate is positioned above the base. Two vertical plates are symmetrically fixed to the bottom of the top plate, with their bottoms fixed to the top of the base. A rotary motor is fixedly mounted at the center of the top of the top plate, and a drive shaft is coaxially fixed to the output shaft of the rotary motor. The bottom of the drive shaft is coaxially fixed to the top of the turntable. By setting a rotatable turntable inside the extraction tank and mounting several extraction columns on it with the help of the vertical support rods, and simultaneously using a rotary motor to drive the drive shaft to rotate the turntable, dynamic rotation of the extraction columns is achieved during the extraction process. This design allows the extraction columns to fully contact the sample solution inside the extraction tank, increasing the contact area and contact frequency, and improving the extraction efficiency of the target substance. Furthermore, by replacing manual operation with automated rotation, the automation level of the pretreatment process is improved, meeting the efficiency and consistency requirements of mass spectrometry for sample pretreatment.

[0006] As a preferred technical solution, a base plate is fixedly installed at the bottom of the extraction tank, and two hydraulic cylinders are symmetrically fixedly installed at the bottom of the top plate. The piston rods of both hydraulic cylinders are fixedly connected to the top of the base plate. The two hydraulic cylinders can drive the extraction tank to move up and down, facilitating the adjustment of the relative position of the extraction tank and the cap. When it is necessary to load or unload the extraction column or add samples, the hydraulic cylinders move the extraction tank downwards for easy operation; when extraction is performed, the hydraulic cylinders move the extraction tank upwards to seal the cap, preventing sample solution leakage or external contamination. Simultaneously, the position of the extraction tank can be flexibly adjusted according to the extraction column height and sample volume, enhancing the adaptability and ease of operation of the device.

[0007] As a preferred technical solution, a connecting block is installed at the center of the bottom of the top plate, and a cylindrical cover is installed at the bottom of the connecting block, with the cylindrical cover facing the extraction tank. This allows for sealing of the extraction tank during the extraction process.

[0008] As a preferred technical solution, the drive shaft passes through the top plate, the connecting block, and the cylinder cover, and is rotatably connected to all three. This rotatable connection ensures stable driving of the turntable by the rotary motor without compromising the sealing function of the cylinder cover over the extraction tank.

[0009] As a preferred technical solution, a fixed plate is integrally formed coaxially at the bottom of the drive shaft. The fixed plate is coaxially fixed to the turntable by a plurality of fixing bolts arranged in a circular array. This enhances the firmness and coaxiality of the connection between the drive shaft and the turntable, while also facilitating the disassembly of the turntable.

[0010] As a preferred technical solution, each of the vertical support rods is threaded with a limiting screw head at its top, and the bottom of the limiting screw head is in close contact with the extraction column. This securely fixes the extraction column to the vertical support rod. During the rotation of the turntable, it prevents the extraction column from shifting or falling off due to centrifugal force or vibration, ensuring that the extraction column remains in a stable working position.

[0011] As a preferred technical solution, a discharge pipe is installed near the bottom of the outer wall of the extraction tank, and a discharge valve is installed on the discharge pipe. The discharge pipe and discharge valve on the outer wall of the extraction tank can realize the centralized discharge of the sample solution after extraction. Waste liquid or extracted solution can be quickly discharged without disassembling the device, simplifying the operation process.

[0012] As a preferred technical solution, a support column is fixedly installed at each of the four corners of the base. The four support columns at the bottom of the base provide stable support for the entire device, raising the height of the device and preventing the base from directly contacting the ground.

[0013] Compared with the prior art, the beneficial effects of this utility model are: This invention achieves dynamic rotation of the extraction columns during the extraction process by incorporating a rotatable turntable within the extraction chamber and mounting several extraction columns on the turntable via vertical support rods. A rotary motor drives a transmission shaft to rotate the turntable. This design ensures sufficient contact between the extraction columns and the sample solution within the extraction chamber, increasing the contact area and frequency, and improving the extraction efficiency of the target substance. Furthermore, the automated rotation drive replaces manual operation, enhancing the automation level of the pretreatment process and meeting the efficiency and consistency requirements of mass spectrometry for sample pretreatment. Attached Figure Description

[0014] Figure 1 This is one of the overall structural schematic diagrams of this utility model; Figure 2 This is the second schematic diagram of the overall structure of this utility model; Figure 3 In this utility model Figure 2 A partial structural diagram; The meanings of the labels in the diagram are as follows: 1. Base; 2. Support column; 3. Top plate; 30. Vertical plate; 4. Hydraulic cylinder; 5. Extraction tank; 50. Bottom plate; 51. Discharge pipe; 52. Discharge valve; 53. Connecting block; 54. Cylinder cover; 55. Drive shaft; 550. Fixing plate; 551. Fixing bolt; 56. Turntable; 57. Limiting screw; 58. Vertical support rod; 6. Rotary motor; 7. Extraction column. Detailed Implementation

[0015] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0016] Please see Figures 1-3 This utility model provides a technical solution: An automated online solid-phase extraction (SPE) device for mass spectrometry sample pretreatment includes an extraction tank 5. Inside the extraction tank 5 is a rotatable turntable 56. Several vertical support rods 58 are fixedly mounted on the top of the turntable 56. An extraction column 7 is fitted onto the outer side of each vertical support rod 58. A base 1 is located below the extraction tank 5, and a top plate 3 is located above the base 1. Two vertical plates 30 are symmetrically fixed to the bottom of the top plate 3, and the bottoms of both vertical plates 30 are fixed to the top of the base 1. A rotary motor 6 is fixedly mounted at the center of the top of the top plate 3. A drive shaft 55 is coaxially fixed to the output shaft of the rotary motor 6, and the bottom of the drive shaft 55 is coaxially fixed to the top of the turntable 56. By setting a rotatable turntable 56 inside the extraction tank 5 and mounting several extraction columns 7 on the turntable 56 with the help of the vertical support rods 58, and simultaneously using the rotary motor 6 to drive the drive shaft 55 to rotate the turntable 56, dynamic rotation of the extraction columns 7 is achieved during the extraction process. This design allows the extraction column 7 to fully contact the sample solution in the extraction tank 5, increasing the contact area and contact frequency, and improving the extraction efficiency of the target substance. At the same time, the automated rotation drive replaces manual operation, improving the automation level of the pretreatment process and meeting the requirements of mass spectrometry for sample pretreatment efficiency and consistency.

[0017] In a preferred embodiment, a base plate 50 is fixedly installed at the bottom of the extraction tank 5, and two hydraulic cylinders 4 are symmetrically fixedly installed at the bottom of the top plate 3. The piston rods of both hydraulic cylinders 4 are fixedly connected to the top of the base plate 50. The two hydraulic cylinders 4 can drive the extraction tank 5 to move up and down, facilitating the adjustment of the relative position of the extraction tank 5 and the cap 54. When it is necessary to load or unload the extraction column 7 or add samples, the hydraulic cylinders 4 drive the extraction tank 5 to move down, facilitating operation; when extraction is performed, the hydraulic cylinders 4 drive the extraction tank 5 to move up, so that the cap 54 seals the extraction tank 5, preventing sample solution leakage or external contamination. At the same time, the position of the extraction tank 5 can be flexibly adjusted according to the height of the extraction column 7 and the sample volume, enhancing the adaptability and ease of operation of the device.

[0018] In a preferred embodiment, a connecting block 53 is installed at the center of the bottom of the top plate 3, and a cylindrical cover 54 is installed at the bottom of the connecting block 53, with the cylindrical cover 54 facing the extraction tank 5. This allows for sealing of the extraction tank 5 during the extraction process.

[0019] In a preferred embodiment, the drive shaft 55 passes through the top plate 3, the connecting block 53, and the cylinder cover 54, and is rotatably connected to all three. This rotatable connection ensures stable driving of the rotary motor 6 to the turntable 56 without affecting the sealing function of the cylinder cover 54 over the extraction tank 5.

[0020] In a preferred embodiment, the bottom of the drive shaft 55 is integrally formed with a fixed plate 550, which is coaxially fixed to the turntable 56 by a plurality of fixing bolts 551 arranged in a circular array. This enhances the firmness and coaxiality of the connection between the drive shaft 55 and the turntable 56, and also facilitates the disassembly of the turntable 56.

[0021] In a preferred embodiment, each vertical support rod 58 is threaded with a limiting screw head 57 at its top, and the bottom of the limiting screw head 57 is in close contact with the extraction column 7. This securely fixes the extraction column 7 to the vertical support rod 58. During the rotation of the turntable 56, this prevents the extraction column 7 from shifting or falling off due to centrifugal force or vibration, ensuring that the extraction column 7 remains in a stable working position.

[0022] In a preferred embodiment, a discharge pipe 51 is installed near the bottom of the outer wall of the extraction tank 5, and a discharge valve 52 is installed on the discharge pipe 51. The discharge pipe 51 and discharge valve 52 on the outer wall of the extraction tank 5 enable centralized discharge of the sample solution after extraction. Waste liquid or extracted solution can be quickly discharged without disassembling the device, simplifying the operation process.

[0023] As a preferred embodiment, a support column 2 is fixedly installed at each of the four corners of the bottom of the base 1. The four support columns 2 at the bottom of the base 1 provide stable support for the entire device, raising the height of the device and preventing the base 1 from directly contacting the ground.

[0024] In use, the automated online solid-phase extraction device for mass spectrometry sample pretreatment of this utility model first retracts the piston rod of the hydraulic cylinder 4 at the bottom of the top plate 3 via the control system, causing the extraction tank 5 to move down and separate from the cover 54. The operator places the extraction column 7 onto the vertical support rod 58 of the turntable 56, fixes it with the limiting screw head 57, and then injects the sample solution. Then, the hydraulic cylinder 4 is controlled to move the extraction tank 5 up and seal it with the cover 54. Next, the rotary motor 6 on the top plate 3 is started. The motor drives the turntable 56 to rotate via the transmission shaft 55 (firmly connected to the turntable 56 through the fixed plate 550), so that the extraction column 7 makes dynamic contact with the sample solution to enhance mass transfer and improve extraction efficiency. During the process, the cover 54 ensures a seal and the transmission shaft 55 provides stable transmission. After extraction is completed, the motor is stopped and the discharge valve 52 of the discharge pipe 51 of the extraction tank 5 is opened to discharge the solution. Then, the hydraulic cylinder 4 is controlled to move the extraction tank 5 down, the limiting screw head 57 is unscrewed to replace the extraction column 7, and the next cycle can begin. The support column 2 of the base 1 always provides stable support for the device.

[0025] The foregoing has shown and described the basic principles, main features, and advantages of this utility model. Those skilled in the art should understand that this utility model is not limited to the above embodiments. The embodiments and descriptions in the specification are merely preferred examples and are not intended to limit the utility model. Various changes and modifications can be made to this utility model without departing from its spirit and scope, and all such changes and modifications fall within the scope of the claimed utility model. The scope of protection of this utility model is defined by the appended claims and their equivalents.

Claims

1. An automated online solid-phase extraction device for mass spectrometry sample pretreatment, characterized in that: The extraction tank (5) includes a rotating turntable (56) installed inside the extraction tank (5). Several vertical support rods (58) are fixedly installed on the top of the turntable (56). An extraction column (7) is sleeved on the outside of each vertical support rod (58). A base (1) is provided below the extraction tank (5). A top plate (3) is provided above the base (1). Two vertical plates (30) are symmetrically fixedly installed at the bottom of the top plate (3). The bottom of the two vertical plates (30) is fixed to the top of the base (1). A rotary motor (6) is fixedly installed at the center of the top of the top plate (3). A transmission shaft (55) is coaxially fixed to the output shaft of the rotary motor (6). The bottom of the transmission shaft (55) is coaxially fixed to the top of the turntable (56).

2. The automated online solid-phase extraction device for mass spectrometry sample pretreatment as described in claim 1, characterized in that: The bottom of the extraction tank (5) is fixedly installed with a base plate (50), and two hydraulic cylinders (4) are symmetrically fixedly installed at the bottom of the top plate (3). The piston rods of the two hydraulic cylinders (4) are fixedly connected to the top of the base plate (50).

3. The automated online solid-phase extraction device for mass spectrometry sample pretreatment as described in claim 2, characterized in that: A connecting block (53) is installed at the center of the bottom of the top plate (3), and a cylinder cover (54) is installed at the bottom of the connecting block (53), which is directly opposite the extraction tank (5).

4. The automated online solid-phase extraction device for mass spectrometry sample pretreatment as described in claim 3, characterized in that: The drive shaft (55) passes through the top plate (3), the connecting block (53) and the cylinder cover (54), and is rotatably connected to all three.

5. The automated online solid-phase extraction device for mass spectrometry sample pretreatment as described in claim 4, characterized in that: The bottom of the drive shaft (55) is integrally formed with a fixed plate (550), which is coaxially fixed to the turntable (56) by a number of fixed bolts (551) arranged in a circular array.

6. The automated online solid-phase extraction device for mass spectrometry sample pretreatment as described in claim 5, characterized in that: Each of the vertical support rods (58) has a limiting screw head (57) threaded on its top, and the bottom of the limiting screw head (57) is in close contact with the extraction column (7).

7. The automated online solid-phase extraction device for mass spectrometry sample pretreatment as described in claim 6, characterized in that: The extraction tank (5) has a discharge pipe (51) installed near the bottom on its outer wall, and a discharge valve (52) is installed on the discharge pipe (51).

8. The automated online solid-phase extraction device for mass spectrometry sample pretreatment as described in claim 7, characterized in that: Each of the four corners of the bottom of the base (1) is fixedly installed with a support column (2).

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