Zebrafish image acquisition device

Abstract

The utility model discloses a zebra fish image acquisition device belongs to biological imaging detection technical field, including imaging component, the imaging component includes the bottom plate, the central part of bottom plate is equipped with the imaging groove, the imaging groove is detachably provided with the axial rotation imaging pipe along the length direction, and the imaging groove is equipped with the sample tube and the recovery pipe who links in series with imaging pipe respectively along the length direction both ends outside. In the scheme, the imaging pipe in the imaging groove is used for fixing zebra fish sample and carries out the detection, and zebra fish sample passes through the sample tube and enters the imaging pipe and carries out the detection, and can be recycled through the recovery pipe after imaging observation, and the imaging pipe in the imaging groove can rotate peripherally, thereby can realize the body position adjustment of zebra fish sample, and 360 degrees dead angle observation of zebra fish sample.

Description

Technical Field

[0001] This utility model relates to an image acquisition device, and more specifically, to a zebrafish image acquisition device. Background Technology

[0002] Zebrafish, with their 87% genetic similarity to humans, transparent embryos (complying with the international 3R principle, replacement, reduction, optimization), high reproductive capacity, and ease of basic editing, are widely used in disease mechanism research and drug screening, gene editing, toxicology analysis, memory, and neurological experiments. Recording changes in zebrafish vital signs is essential in most studies. Currently, the main methods for zebrafish fixation and imaging are gel fixation and microfluidic chip fixation. However, neither of these methods can effectively adjust the zebrafish's posture and position, and they also suffer from unclear imaging or the risk of damaging the zebrafish, making continuous observation and imaging of zebrafish ineffective.

[0003] For example, Chinese Patent Publication No. CN214097178U, published on August 31, 2021, discloses a utility model entitled "A Microplate for Convenient Live Imaging of Zebrafish Juveniles." This application discloses a fixing structure for zebrafish detection, including a horizontally placed glass slide to support the zebrafish juveniles and a water film; a PDMS membrane naturally covering the glass slide; the thickness of the PDMS membrane is 1mm ± 0.1mm; the PDMS membrane has several penetrating holes, the diameter of which is adapted to the body length of the zebrafish juveniles; the holes and the glass slide below form a sample slot for placing the zebrafish juveniles and the water layer. While the above solution involves readily available raw materials and convenient assembly, enabling simultaneous live imaging and high-speed motion photography of zebrafish juveniles, it cannot effectively adjust the zebrafish's body position and posture, and therefore cannot provide comprehensive observation and detection of the zebrafish. Utility Model Content

[0004] This invention overcomes the problem that existing image acquisition devices cannot effectively adjust the body position of zebrafish after fixing them, and provides a zebrafish image acquisition device. In the process of image acquisition of zebrafish, this solution can adjust the body position of zebrafish with a relatively fixed position, so as to realize all-round imaging detection and observation of zebrafish.

[0005] To solve the above-mentioned technical problems, this utility model adopts the following technical solution: a zebrafish image acquisition device, including an imaging component, the imaging component including a base plate, an imaging groove provided in the center of the base plate, an axially rotatable imaging tube detachably provided along the length direction of the imaging groove, and a sample loading tube and a recovery tube respectively provided at both ends of the imaging groove along the length direction, connected in series with the imaging tube. In this solution, the imaging tube in the imaging groove is used to fix the zebrafish sample for detection. The zebrafish sample enters the imaging tube through the sample loading tube for detection. After imaging observation, it can be recovered through the recovery tube. The imaging tube in the imaging groove can rotate circumferentially, thereby realizing the adjustment of the zebrafish sample's position and enabling 360° observation of the zebrafish sample without blind spots.

[0006] Preferably, the sample loading tube is equipped with a first driving device at the end near the imaging slot, and the recovery tube is equipped with a second driving device at the side near the imaging slot. The first driving device and the second driving device drive the sample loading tube and the recovery tube to rotate, respectively. The first driving device and the second driving device need to synchronously control the transmission of the sample loading tube and the recovery tube, thereby synchronously driving the imaging tube to rotate, so as to realize the body position adjustment of the zebrafish sample and realize the all-round imaging detection of the zebrafish.

[0007] Preferably, a detection sensor is also provided in the width direction of the imaging slot, and a detection window corresponding to the detection sensor is provided in the width direction of the imaging slot. The detection sensor is used to detect the position of the zebrafish sample inside the imaging tube, so as to adjust the position of the zebrafish sample to be within the imaging range, and the detection window is used to avoid the detection sensor.

[0008] Preferably, pressure sensors are provided on both the sample loading tube and the recovery tube near the imaging slot. These pressure sensors can detect the pressure inside the sample loading tube and the recovery tube, thus providing a comprehensive assessment of the pressure inside the imaging tube. This prevents excessive pressure inside the imaging tube from affecting the vital signs of the zebrafish sample and leading to inaccurate imaging results.

[0009] Preferably, the system also includes an imager. The base plate of the imaging component is mounted on the platform of the imager. The imager is equipped with a first driving mechanism and a second driving mechanism. The base plate is mounted on the first driving mechanism, and the first driving mechanism is mounted on the second driving mechanism. The driving directions of the first driving mechanism and the second driving mechanism are perpendicular to each other. The first driving mechanism and the second driving mechanism can drive the base plate of the imaging component to move on the surface of the imager platform, thereby changing the position of the imaging component on the imager platform to accurately locate the position of the zebrafish sample and perform imaging observation.

[0010] Preferably, the base plate has a clearance hole in the width direction of the imaging slot, and the detection sensor is disposed in the clearance hole and fixedly arranged on the platform. The clearance hole on the base plate is used to avoid the detection sensor fixed on the imaging platform, so that the base plate can be normally adjusted on the imaging platform to locate the position of the zebrafish sample for imaging observation.

[0011] Preferably, the sample loading chamber is also included, which contains a stirring assembly and a driving assembly. The stirring assembly includes a base and a stirring shaft passing through the base. One end of the stirring shaft is connected to the driving assembly via a transmission belt, and the other end of the stirring shaft is built into the sample loading chamber fixed on the base. The driving assembly can drive the stirring shaft of the stirring assembly to rotate, thereby mixing the zebrafish sample in the sample loading chamber, ensuring smooth sample loading and improving sample loading efficiency.

[0012] Preferably, the stirring shaft is a hollow shaft with a sample loading channel inside. The end of the sample loading channel away from the sample loading chamber is connected to the sample loading tube, and the stirring shaft, with stirring blades at the end inside the sample loading chamber, can guide the zebrafish sample from the sample loading chamber into the sample loading tube, and then send it to the imaging tube in the imaging tank for imaging observation. The stirring blades on the stirring shaft can evenly stir the zebrafish in the sample loading chamber, preventing the zebrafish from accumulating and affecting the sample loading operation of the sample loading tube.

[0013] Preferably, the sample loading chamber is also equipped with a drive pump, and the recovery pipe is equipped with a flow path control valve. The drive pump and the flow path control valve are connected through a connecting pipe. The drive pump is the power device for loading zebrafish. Through the cooperation of the flow path control valve and the drive pump, the loading and recovery operations of zebrafish can be realized.

[0014] Preferably, a control board is also provided inside the sample loading chamber. The control board inside the sample loading chamber is a circuit board, which can start the image acquisition device and control the normal operation of the imaging component and stirring component.

[0015] Compared with the prior art, the beneficial effects of this utility model are: (1) It can realize the body position adjustment of zebrafish samples and observe zebrafish samples from 360° without dead angles; (2) It causes less damage to zebrafish samples, which can improve the survival rate of zebrafish after the experiment and realize continuous observation of zebrafish; (3) It can accurately locate the position of zebrafish samples, improve the detection and observation efficiency, and obtain clearer image acquisition. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the overall design of this utility model.

[0017] Figure 2 This is a schematic diagram of the imaging component structure of this utility model.

[0018] Figure 3 This is a top view of the imaging component of this utility model.

[0019] Figure 4 This is an enlarged schematic diagram of the inside of the imaging groove of this utility model.

[0020] Figure 5 This is a schematic diagram of the imaging component and driving mechanism of this utility model.

[0021] Figure 6 This is a schematic diagram of the internal structure of the sample box of this utility model.

[0022] Figure 7 This is a top view of the stirring assembly of this utility model.

[0023] Figure 8 for Figure 7 Sectional view along the AA direction.

[0024] Figure 9 This is a schematic diagram of the structure of this utility model.

[0025] In the diagram: 1. Base plate, 2. Imaging tank, 3. Imaging tube, 4. Sample loading tube, 5. Recovery tube, 6. First drive device, 7. Second drive device, 8. Detection sensor, 9. Detection window, 10. Pressure sensor, 11. Imager, 12. First drive mechanism, 13. Second drive mechanism, 14. Clearance hole, 15. Platform, 16. Sample loading box, 17. Stirring shaft, 18. Base, 19. Transmission belt, 20. Drive assembly, 21. Sample loading chamber, 22. Sample loading channel, 23. Stirring blade, 24. Drive pump, 25. Flow path control valve, 27. Control board, 28. Microscope lens, 29. Focusing knob, 30. Z-shaped support, 31. Adjustment knob, 32. Transmission seat. Detailed Implementation

[0026] The technical solution of this utility model will be further described in detail below through specific embodiments and in conjunction with the accompanying drawings.

[0027] Example 1: As Figures 1 to 9 The zebrafish image acquisition device shown includes an imaging component and a stirring component. The imaging component and the stirring component are arranged adjacent to each other and are connected by a sample loading tube 4. The stirring component is used to stir the zebrafish sample evenly and then supply it to the imaging component through the sample loading tube 4. The imaging component is used for the detection and observation of the zebrafish sample.

[0028] Specifically, such as Figures 1 to 4As shown, the imaging assembly is arranged on platform 15 of imager 11. A microscope lens 28 is mounted on top of imager 11, and a horizontal platform 15 is mounted on the base of imager 11. Focusing knobs 29 are located on both sides of the base of imager 11. These knobs adjust the imaging sharpness of the microscope lens 28, thereby acquiring clear images of the zebrafish sample. Furthermore, the imaging assembly includes a base plate 1 and an imaging groove 2. The base plate 1 is a rectangular thin plate used to support the imaging groove 2. The imaging groove 2 is also a cuboid groove structure, located in the middle of the base plate 1. An imaging tube 3 is arranged inside the imaging groove 2 along its length. The imaging tube 3 is a capillary structure that can accommodate and allow the zebrafish sample to pass through. The imaging tube 3 and the imaging groove 2 are detachably connected, and a bearing structure is provided at the connection point, allowing the imaging tube 3 to rotate circumferentially. When using imager 11 to acquire zebrafish images, the position of the zebrafish sample can be adjusted by rotating the imaging tube 3. Imager 11 can then acquire zebrafish sample images in different positions, improving the accuracy of the observation results.

[0029] Furthermore, a sample loading tube 4 and a recovery tube 5 are respectively provided at both ends of the imaging tank 2 along its length. Both the sample loading tube 4 and the recovery tube 5 are connected to the imaging tube 3. The other end of the sample loading tube 4 is also connected to the stirring assembly, and the other end of the recovery tube 5 is connected to the sample recovery device (not shown in the figure). The connection between the imaging tube 3 and the sample loading tube 4 and the recovery tube 5 is coaxially arranged, and the rotation of the imaging tube 3 can be achieved simply by rotating the sample loading tube 4 and the recovery tube 5 outside the imaging tank 2. Specifically, a first driving device 6 and a second driving device 7 are also provided on the base plate 1. The first driving device 6 and the second driving device 7 are respectively arranged at both ends of the imaging slot 2 along its length and correspond to the positions of the sample tube 4 and the recovery tube 5, respectively. Both the first driving device 6 and the second driving device 7 are rotary motors. Both the first driving device 6 and the second driving device 7 are fixed to the base plate 1 by a Z-shaped bracket 30. The bottom of the Z-shaped bracket 30 is connected to the base plate 1 by screws or other fasteners. The first driving device 6 or the second driving device 7 is arranged at the upper end of the Z-shaped bracket 30, and the sample tube 4 and the recovery tube 5 are respectively located below the first driving device 6 and the second driving device 7. Since the rotation method of the sample tube 4 driven by the first driving device 6 is the same as the rotation method of the recovery tube 5 driven by the second driving device 7, only the linkage between the first driving device 6 and the sample tube 4 will be described here.

[0030] The output end of the first driving device 6 is arranged downwards, and a set of bevel gears arranged vertically are provided at the output end. A set of bevel gears arranged horizontally are fitted around the outside of the upper sample tube 4. The bevel gears on the first driving device 6 mesh with the bevel gears on the upper sample tube 4. When the first driving device 6 is activated, it drives the upper sample tube 4 to rotate circumferentially. When it is necessary to drive the imaging tube 3 to rotate, only the first driving device 6 and the second driving device 7 need to operate synchronously. Couplings are also provided between the upper sample tube 4 and the imaging tube 3, and between the recovery tube 5 and the imaging tube 3, for connecting the upper sample tube 4, the imaging tube 3, and the recovery tube 5. Sealing is required at the connection points.

[0031] Furthermore, two sets of pressure sensors 10 are arranged on the base plate 1. The two sets of pressure sensors 10 are respectively arranged on the side of the sample tube 4 and the recovery tube 5 near the imaging tank 2. The pressure sensors 10 can detect the pressure in the sample tube 4 and the recovery tube 5, thereby understanding the pressure in the imaging tube 3, and avoiding excessive pressure in the imaging tube 3 from affecting the vital signs of the zebrafish sample.

[0032] Since the stirring assembly's movement is controlled by the control board 27, and there is a delay in signal transmission from various sensing components, the zebrafish sample may not be within the effective detection range after entering the imaging tube 3. In this case, it is necessary to adjust the position of the imaging assembly on the imager 11 platform 15 to reposition the zebrafish sample within the imager 11's detection range. Therefore, a driving mechanism is also provided between the imager 11 platform 15 and the imaging assembly. This driving mechanism includes a first driving mechanism 12 and a second driving mechanism 13. The first driving mechanism 12 and the second driving mechanism 13 are driving mechanisms in two dimensions on the platform 15, used to drive the imaging assembly to move in both the horizontal and vertical dimensions (the frontal view direction of the imager 11) of the imager 11 platform 15, thereby enabling the imaging assembly to be horizontally adjusted on the imager 11 platform 15. Specifically, as... Figure 5 As shown, the first drive mechanism 12 is a lead screw transmission structure. An adjustment knob 31 is provided at the outer end of the lead screw, and a transmission seat 32 is provided in the middle of the lead screw. The base plate 1 is fixed on the transmission seat 32. When the adjustment knob 31 is rotated, the transmission seat 32 can be driven to move longitudinally, which in turn causes the base plate 1 to move longitudinally, thus realizing the longitudinal adjustment of the imaging component on the platform 15. Furthermore, the first drive mechanism 12 is arranged on the second drive mechanism 13. The second drive mechanism 13 is a gear and rack transmission structure, wherein the rack is arranged on the second drive mechanism 13, and the gear can be arranged on the platform 15. In this way, the rack can be driven to move through the gear, thereby driving the first drive mechanism 12 on the second drive mechanism 13 to move in the horizontal direction, thus realizing the horizontal adjustment of the imaging component.

[0033] Furthermore, detection sensors 8 are also provided on both sides of the imaging slot 2 in the width direction. Detection sensors 8 are two through-beam sensors, one for transmitting signals and the other for receiving signals, used to detect the sample passage status in the imaging tube 3, thereby fixing the position of the zebrafish sample. To avoid misjudgment, waist-shaped detection windows 9 are provided on both side walls of the imaging slot 2 in the width direction, and transparent glass plates are arranged on the detection windows 9 to avoid interference. When the imaging assembly is moved and adjusted, the detection sensors 8 do not need to move. The position of the detection sensors 8 relative to the imager 11 is pre-adjusted; therefore, the detection sensors 8 need to be fixed on the platform 15 of the imager 11, while also avoiding the base plate 1 so that the imaging assembly can be adjusted.

[0034] Specifically, two sets of clearance holes 14 are provided on the base plate 1, respectively arranged on both sides of the imaging slot 2 in the width direction. The clearance holes 14 are rectangular through holes, and the detection sensor 8 is located inside the clearance holes 14. The size of the clearance holes 14 is larger than the size of the detection sensor 8, thus allowing for a certain adjustment range between the base plate 1 and the platform 15. When the zebrafish sample in the imaging tube 3 is detected again by the detection sensor 8, the adjustment can be stopped. The specific adjustment method can be adjusted through computer control software (jog control combined with long press control), or it can be controlled by rotating the adjustment knob 31 or other structures.

[0035] Furthermore, such as Figures 6 to 9 As shown, the stirring assembly is located inside the sample loading chamber 16, which is arranged adjacent to the imager 11. The stirring assembly includes a base 18, a stirring shaft 17, and a sample loading chamber 21. Specifically, the stirring shaft 17 extends vertically through the base 18, and both the upper and lower ends of the stirring shaft 17 are located outside the base 18. The sample loading chamber 21 is located below the base 18. The sample loading chamber 21 is a cylindrical or conical cup structure. A buckle is provided at the top of the sample loading chamber 21. An annular groove that matches the buckle at the top of the sample loading chamber 21 is provided on the lower surface of the base 18, so that a detachable connection can be formed between the sample loading chamber 21 and the base 18, which facilitates sample loading and sample changing. Furthermore, the lower end of the stirring shaft 17 is located inside the sample loading chamber 21, which contains zebrafish samples (clear water and juvenile zebrafish). The lower end of the stirring shaft 17 is also equipped with stirring blades 23. These blades are spiral-shaped and made of medical-grade 316L stainless steel, with a polished and specially treated surface to prevent secondary damage to the zebrafish samples and sample adhesion to the sample walls. It should be noted that the stirring blades 23 and the stirring shaft 17 are detachably connected, allowing for easy adjustment of the stirring blades according to different samples.

[0036] The sample loading chamber 16 also houses a drive assembly 20 and a control board 27. The drive assembly 20 is a servo motor, with a drive wheel at its top. Furthermore, a driven wheel is located at the upper end of the stirring shaft 17. The drive wheel and driven wheel are connected by a transmission belt 19. When the drive assembly 20 operates, it drives the drive wheel to rotate, which in turn drives the driven wheel via the transmission belt 19, thus achieving the stirring operation of the stirring shaft 17. The stirring by the stirring shaft 17 thoroughly mixes the zebrafish sample in the sample loading chamber 21, preventing sample accumulation and blockage of the sample loading tube 4, and ensuring that the zebrafish can smoothly enter the imaging tube 3. Only single-tailed juvenile zebrafish can pass through the imaging tube 3. The control board 27 is an integrated circuit board that controls the operation of the stirring assembly, drive assembly 20, and drive pump 24.

[0037] Furthermore, the stirring shaft 17 is a hollow shaft, and an axial sample loading channel 22 is formed inside the stirring shaft 17. The bottom of the sample loading channel 22 connects to the interior of the sample loading chamber 21, and the top of the sample loading channel 22 connects to the sample loading tube 4. A drive pump 24 is also installed inside the sample loading chamber 16. The drive pump 24 is used to draw the zebrafish sample in the sample loading chamber 21 into the imaging tube 3. In order to prevent damage to the zebrafish sample, the drive pump 24 needs to be arranged outside the pipeline. The sample loading chamber 21, sample loading channel 22, sample loading tube 4, imaging tube 3 and recovery tube 5 are connected in sequence. The interior of the sample loading chamber 21 is in a closed state. Therefore, the drive pump 24 can only be connected to the side closest to the recovery tube 5. Since the drive pump 24 needs to be arranged outside the pipeline, the drive pump 24 is a piston pump. The forward movement of the piston in the drive pump 24 draws the zebrafish sample in the sample loading chamber 21 into the sample loading tube 4 and the imaging tube 3. When the detection sensor 8 detects the zebrafish sample, the drive pump 24 stops working. Because a negative pressure is created inside the drive pump 24, the zebrafish sample in the imaging tube 3 remains stationary, allowing the imager 11 to acquire an image of the zebrafish sample. When the piston inside the drive pump 24 moves in the reverse direction, the zebrafish is discharged through the recovery tube 5 into the sample recovery device.

[0038] Specifically, a flow path control valve 25 is also installed on the base plate 1. The flow path control valve 25 is arranged on one side of the recovery pipe 5, and the drive pump 24 is connected to the flow path control valve 25 through a connecting pipe. The flow path control valve 25 is a clamp valve. The connecting pipe and the recovery pipe 5 are connected in parallel to form a Y-shaped pipeline. One end of the recovery pipe 5 is connected in series with the imaging pipe 3 in the imaging tank 2, and the other end of the recovery pipe 5 is connected to the sample recovery device. The connecting pipe connects to the middle position of the recovery pipe 5. The two clamping and shut-off positions of the flow path control valve 25 are located on the recovery pipe 5 and on both sides of the connecting pipe. Here, the side of the recovery pipe 5 closer to the imaging tank 2 is defined as the upstream of the flow path control valve 25, and the upstream clamping and shut-off of the flow path control valve 25 is defined as the upstream of the recovery pipe 5; the side of the recovery pipe 5 farther from the imaging tank 2 is defined as the downstream of the flow path control valve 25, and the downstream clamping and shut-off of the flow path control valve 25 is defined as the downstream of the recovery pipe 5. During the sample loading process, the flow path control valve 25 first shuts off downstream. At this time, the connecting pipe is connected to the upstream of the recovery pipe 5. That is, the drive pump 24 connects the upstream of the recovery pipe 5, the imaging pipe 3, the sample loading pipe 4, and the sample loading chamber 21. The piston in the drive pump 24 moves forward to produce a suction effect on the zebrafish sample in the sample loading chamber 21, so that the mixed zebrafish sample enters the imaging pipe 3 through the sample loading channel 22 in the stirring shaft 17 and the sample loading pipe 4. When the zebrafish reaches the imaging pipe 3 and is detected, the drive pump 24 stops working and can keep the zebrafish sample in the imaging pipe 3, thus completing the sample loading operation.

[0039] During sample recovery, it is also necessary to prevent zebrafish samples from entering the drive pump 24 and causing damage. It is crucial to prevent zebrafish from entering deep into the connecting tube. Therefore, a blocking device is installed inside the connecting tube to keep the zebrafish samples within the recovery tube 5 or to allow them to enter only a small portion of the connecting tube without approaching the drive pump 24. In this embodiment, the blocking device can be a filter screen structure, which can block juvenile zebrafish without isolating the liquid. Before sample recovery, the zebrafish samples are imaged and acquired in the imaging tank 2 and remain in the imaging tube 3. The drive pump 24 needs to continue pumping the zebrafish samples into the recovery tube 5 in its original direction (piston forward movement). At this time, the flow path control valve 25 is still in the downstream cutoff position. When all the zebrafish samples are in the downstream cutoff position (or in a small portion of the connecting tube), the flow path control valve 25 is adjusted to the upstream cutoff position. At this time, the connecting tube and the downstream of the recovery tube 5 are connected, meaning the drive pump 24 is connected to the downstream of the recovery tube 5. The piston inside the drive pump 24 moves in the reverse direction, pushing the water and zebrafish samples together into the sample recovery device. In practice, the sample recovery device can use the same cup as the sample loading chamber 21 to facilitate the next sample loading.

Claims

1. A zebrafish image acquisition device, characterized in that, The device includes an imaging assembly, which includes a base plate. An imaging groove is provided in the center of the base plate. An axially rotatable imaging tube is detachably provided in the imaging groove along its length. A sample loading tube and a recovery tube connected in series with the imaging tube are respectively provided at both ends of the imaging groove along its length.

2. The zebrafish image acquisition device according to claim 1, characterized in that, The sample loading tube is provided with a first driving device at one end near the imaging slot, and the recovery tube is provided with a second driving device at one side near the imaging slot. The first driving device and the second driving device respectively drive the sample loading tube and the recovery tube to rotate.

3. The zebrafish image acquisition device according to claim 1, characterized in that, A detection sensor is also provided in the width direction of the imaging slot, and a detection window corresponding to the detection sensor is provided in the width direction of the imaging slot.

4. The zebrafish image acquisition device according to claim 1, characterized in that, Pressure sensors are provided on both the sample loading tube near the imaging slot and the recovery tube near the imaging slot.

5. The zebrafish image acquisition device according to claim 3, characterized in that, It also includes an imager, the base plate of the imaging component is disposed on the platform of the imager, the imager is provided with a first driving mechanism and a second driving mechanism, the base plate is disposed on the first driving mechanism, the first driving mechanism is disposed on the second driving mechanism, and the driving directions of the first driving mechanism and the second driving mechanism are perpendicular to each other.

6. The zebrafish image acquisition device according to claim 5, characterized in that, The base plate has a clearance hole in the width direction of the imaging slot, and the detection sensor is disposed in the clearance hole and fixedly arranged on the platform.

7. A zebrafish image acquisition device according to any one of claims 1 to 6, characterized in that, It also includes a sample loading chamber, which contains a stirring assembly and a driving assembly. The stirring assembly includes a base and a stirring shaft that passes through the base. One end of the stirring shaft is connected to the driving assembly via a transmission belt, and the other end of the stirring shaft is built into the sample loading chamber fixed on the base.

8. The zebrafish image acquisition device according to claim 7, characterized in that, The stirring shaft is a hollow shaft and forms a sample loading channel inside. The end of the sample loading channel away from the sample loading chamber is connected to the sample loading tube. The stirring shaft is equipped with stirring blades at the end built into the sample loading chamber.

9. A zebrafish image acquisition device according to claim 7, characterized in that, The sample loading chamber is also equipped with a drive pump, and the recovery pipe is equipped with a flow path control valve. The drive pump and the flow path control valve are connected through a connecting pipe.

10. A zebrafish image acquisition device according to claim 7, characterized in that, The sample loading box is also equipped with a control panel.

Images