A plant tissue culture bottle

Abstract

The utility model relates to a kind of plant tissue culture bottles, including glass bottle body, bottle cap being installed in the top of glass bottle body, magnetic cover being installed in the top of bottle cap, the upper end surface of bottle cap is equipped with installation slot, the bottom of installation slot is equipped with air hole, the inside of installation slot is provided with air filter membrane, air filter membrane is used to filter microorganism and particulate matter in external air, the upper end surface of magnetic cover is equipped with eccentric hole, the upper end surface of magnetic cover is located the outside of eccentric hole and is equipped with outlet micro-porous, magnetic cover and bottle cap between being provided with magnetic attraction component;The utility model is through the eccentric hole, air hole linkage adjusting structure between magnetic cover and bottle cap, realized the continuous or grading dynamic control of ventilation volume, rotates magnetic cover, changes the overlapping area between eccentric hole and air hole, changes ventilation area, to adapt to the demand of plant tissue in different growth stages to gas environment, improves the controllability and adaptability of culture process.

Description

Technical Field

[0001] This utility model relates to the field of culture bottle technology, specifically a plant tissue culture bottle. Background Technology

[0002] Plant tissue culture is one of the core technologies in modern biotechnology. It is widely used in fields such as rapid propagation of seedlings, detoxification treatment, genetic transformation, germplasm resource preservation, and production of secondary metabolites of medicinal plants. During tissue culture, the growth and differentiation of explants not only depend on suitable culture medium components and hormone ratios, but also on stable physical environmental conditions. Among them, temperature, light, humidity, and gas exchange (especially the balance of oxygen and carbon dioxide) are key factors affecting cell respiration, metabolic activity, and morphogenesis. For example, in olive tissue culture propagation technology, culture flasks are required for primary culture, subculture proliferation culture, and rooting culture of explants. Currently, conventional culture flasks have ventilation holes on their caps for easy aeration. However, existing plant tissue culture flasks cannot dynamically adjust the gas supply according to different growth stages of the plant, have a relatively simple structure, and require the addition of a filtration structure when aerating. Generally, the filter membrane is placed at the bottle mouth before installing the cap, but the replacement process is cumbersome and can easily damage the seal. Based on this, we propose a plant tissue culture flask to solve the above problems. Summary of the Invention

[0003] To address the shortcomings of existing technologies, this utility model provides a plant tissue culture bottle that enables continuous or graded adjustment of aeration. This solves the problems of existing plant tissue culture bottles being unable to dynamically adjust gas supply according to different growth stages of plants, having a relatively simple structure, and requiring the addition of a filtration structure during aeration. Generally, the filter membrane is placed at the bottle mouth before installing the bottle cap, but the replacement process is cumbersome and easily damages the seal.

[0004] To achieve the above objectives, this utility model provides the following technical solution: a plant tissue culture bottle, comprising a glass bottle body, a bottle cap mounted on the top of the glass bottle body, and a magnetic cap fitted to the top of the bottle cap. The upper surface of the bottle cap has an installation groove, and the bottom of the installation groove has a vent hole. An air filter membrane is provided inside the installation groove to filter microorganisms and particulate matter from the outside air. The upper surface of the magnetic cap has an eccentric hole, and the upper surface of the magnetic cap, located outside the eccentric hole, has a micro-vent hole. A magnetic assembly is provided between the magnetic cap and the bottle cap for detachable connection. By rotating the magnetic cap, the overlap area between the eccentric hole and the vent hole changes, thereby adjusting the gas flow rate into the bottle.

[0005] Furthermore, the surface of the glass bottle is equipped with a scale, the cap and the glass bottle are connected by a threaded seal, and a sealing rubber ring is provided on the top of the inner side of the cap.

[0006] Furthermore, the outer wall of the magnetic cap is equipped with a rotation indicator, and the outer wall of the bottle cap is equipped with a corresponding status indicator, which includes ventilation status scales of closed, low, medium and high, to intuitively display the current ventilation status.

[0007] Furthermore, the air filter membrane is a hydrophobic polytetrafluoroethylene microporous filter membrane with a pore size of .~.μm, and the outer diameter of the air filter membrane matches the mounting groove, is tightly embedded in the mounting groove, and covers the vent holes.

[0008] Furthermore, the eccentric hole is a fan-shaped through hole, and the geometric center of the eccentric hole is offset from the rotation center of the magnetic cover. When the magnetic cover rotates around its central axis, the overlapping area of ​​the eccentric hole and the vent hole changes, forming multiple settings such as closed, low ventilation, medium ventilation and high ventilation.

[0009] Furthermore, the exhaust micro-holes are distributed in a ring array along the edge of the magnetic cover, and all the exhaust micro-holes are tapered structures with a smaller top and a larger bottom, which are used to assist in exhaust.

[0010] It is important to note that the air intake (eccentric hole-vent) and air outlet (conical micro-outlet) paths are separated, forming a directional airflow circulation, which effectively avoids gas retention and CO2 accumulation caused by a single opening.

[0011] Furthermore, the magnetic suction assembly includes a first permanent magnet fixedly installed on the bottom edge of the magnetic cap, a corresponding engagement groove opened on the upper edge of the bottle cap, and a second permanent magnet fixedly installed at the bottom of the engagement groove. The first permanent magnet and the engagement groove correspond one-to-one. When the first permanent magnet is inserted into the engagement groove, the first permanent magnet and the second permanent magnet attract each other, so that the magnetic cap fits tightly against the bottle cap.

[0012] It should be noted that the overlap adjustment between the eccentric hole and the vent hole is achieved through magnetic attraction. After removing the magnetic cap, simply adjust its orientation and reinstall it. The meshing grooves are distributed in a ring, such as 8 meshing grooves, with an angle of 45 degrees between two meshing grooves, which facilitates adjustment and control. This also makes it easy to adapt to the independent installation of the air filter membrane, ensuring that the entire bottle cap does not need to be disassembled or the sealing structure damaged when replacing the filter membrane. At the same time, compared with conventional rotation adjustment, the internal structure is simplified, making it easier to clean and disinfect the bottle cap.

[0013] Furthermore, the magnetic cover is completely detachable, the mounting slot is an open structure, and the outer wall of the magnetic cover is provided with anti-slip texture.

[0014] Compared with the prior art, the technical solution of this application has the following beneficial effects: 1. This plant tissue culture bottle, through the setting of an eccentric hole-ventilation hole linkage adjustment structure between the magnetic cap and the bottle cap, realizes continuous or graded dynamic control of the ventilation volume. By rotating the magnetic cap, the overlap area between the eccentric hole and the ventilation hole is changed, thereby changing the ventilation area, thus adapting to the gas environment requirements of plant tissues at different growth stages such as callus induction, proliferation culture, and root differentiation, and improving the controllability and adaptability of the culture process; 2. This plant tissue culture bottle adopts a modular magnetic connection structure and an independent installation slot design, which makes the air filter membrane easy to replace and the whole bottle easy to clean and disinfect. The magnetic cap can be completely removed, and the installation slot has an open structure, which makes it easy to remove the aged or clogged filter membrane and replace it with a new one. 3. The plant tissue culture bottle features conical venting micropores arranged in a ring to promote the directional discharge of CO2 and moisture, forming a beneficial gas circulation. The magnetic cap has a rotation indicator on its outer wall, which, together with the ventilation status scale on the cap, allows for visual operation of the ventilation level. Attached Figure Description

[0015] Figure 1 The diagram shown is a schematic representation of the overall structure of this utility model. Figure 2 The diagram shown is a schematic representation of the internal structure of this utility model. Figure 3 This utility model is shown. Figure 2 Enlarged structural diagram at point A in the middle; Figure 4 The diagram shown is a schematic of the eccentric hole structure of this utility model. Figure 5 The diagram shown is a schematic of the magnetic cap structure of this utility model. Figure 6 The diagram shown is a schematic diagram of the bottle cap structure of this utility model.

[0016] Explanation of reference numerals in the attached drawings: 1. Glass bottle body; 2. Bottle cap; 3. Magnetic cap; 31. First permanent magnet; 32. Engaging groove; 33. Second permanent magnet; 4. Mounting groove; 5. Vent hole; 6. Air filter membrane; 7. Eccentric hole; 8. Exhaust micro-hole; 9. Rotation indicator. Detailed Implementation

[0017] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0018] Please see Figures 1-6This embodiment of a plant tissue culture flask includes a glass bottle body 1, a bottle cap 2 mounted on the top of the glass bottle body 1, and a magnetic cap 3 fitted onto the top of the bottle cap 2. The upper surface of the bottle cap 2 has a mounting groove 4. A scale is provided on the surface of the glass bottle body 1. The bottle cap 2 and the glass bottle body 1 are connected by a threaded seal, and a sealing rubber ring is provided on the top inner side of the bottle cap 2. A vent hole 5 is provided at the bottom of the mounting groove 4, and an air filter membrane 6 is provided inside the mounting groove 4 for filtering external air. For the removal of microorganisms and particulate matter, the upper surface of the magnetic cap 3 is provided with an eccentric hole 7, and the upper surface of the magnetic cap 3 is provided with a venting micro-hole 8 outside the eccentric hole 7. A magnetic suction assembly is provided between the magnetic cap 3 and the bottle cap 2. The magnetic suction assembly is used for the detachable connection between the magnetic cap 3 and the bottle cap 2. By rotating the magnetic cap 3, the overlapping area between the eccentric hole 7 and the venting hole 5 changes, thereby adjusting the gas flow rate into the bottle. The magnetic cap 3 can be completely removed, and the mounting groove 4 is an open structure. The outer wall of the magnetic cap 3 is provided with anti-slip texture.

[0019] It should be noted that the geometric center of the eccentric hole 7 is offset from the rotation center of the magnetic cover 3. When the magnetic cover 3 rotates around its central axis, the overlapping area between it and the vent hole 5 gradually increases from zero to the maximum, forming multiple stable positions from "closed" to "high ventilation". This avoids the technical defects of traditional fixed hole diameter or cotton plug sealing that cannot be adjusted as needed, and improves the adaptability to the gas requirements of different culture stages.

[0020] In this embodiment, the venting micropores 8 are arranged in a ring array along the edge of the magnetic cap 3, and all the venting micropores 8 are tapered structures with a smaller top and a larger bottom. The venting micropores 8 are used to assist in exhaust. This design has a dual function: on the one hand, it can reduce the risk of reverse intrusion of external dust and droplets, playing a physical protection role; on the other hand, it allows the CO2 and moisture accumulated in the bottle to be smoothly discharged under the action of internal and external pressure difference, forming a directional airflow, promoting gas renewal, and preventing growth inhibition or vitrification caused by the accumulation of metabolic products.

[0021] It should be noted that the design of separating the air intake and exhaust paths effectively improves the gas exchange efficiency. Fresh air enters through the eccentric hole 7 and the vent 5, and enters the bottle after being filtered by the air filter membrane 6. Meanwhile, the exhaust gas in the bottle is passively discharged mainly through the annularly distributed exhaust micropores 8. The two are spatially separated, avoiding short-circuit backflow and enhancing the rationality and functionality of the ventilation system. The conical structure can also slow down the airflow speed to a certain extent, reduce the drastic disturbance to the humidity inside the bottle, and maintain a relatively stable microenvironment.

[0022] Please see Figure 1 , Figure 4 and Figure 6In this embodiment, the outer wall of the magnetic cap 3 is provided with a rotation indicator 9, and the outer wall of the bottle cap 2 is provided with a corresponding status indicator. The status indicator includes ventilation status scales of closed, low, medium and high, which are used to intuitively display the current ventilation status. The air filter membrane 6 is a hydrophobic polytetrafluoroethylene microporous filter membrane with a pore size of 0.1 to 0.45 μm. The outer diameter of the air filter membrane 6 matches the mounting groove 4, is tightly embedded in the mounting groove 4 and covers the ventilation hole 5. The eccentric hole 7 is a fan-shaped through hole. The geometric center of the eccentric hole 7 is offset from the rotation center of the magnetic cap 3. When the magnetic cap 3 rotates around its central axis, the overlapping area of ​​the eccentric hole 7 and the ventilation hole 5 changes, forming multiple positions of closed, low ventilation, medium ventilation and high ventilation.

[0023] It should be noted that there is a synergistic relationship among the above structural features. The accuracy of the rotating indicator 9 depends on the stable positioning capability provided by the magnetic component. The effective protection of the air filter membrane 6 depends on the continuous coverage of the magnetic cover 3 during the adjustment process. The repeatability of ventilation control is based on the precise alignment of the eccentric hole 7 and the ventilation hole 5. These components do not exist in isolation, but constitute a complete and logically closed-loop gas management system.

[0024] In this embodiment, the eccentric hole 7 is an array of circular holes, and the distribution of the circular holes is adapted to the bottom vent hole 5. When the magnetic cover 3 is rotated, the circular holes and the vent hole 5 are misaligned, and the overlapping area of ​​the eccentric hole 7 and the vent hole 5 changes more evenly and smoothly, which further improves the precision and stability of ventilation control.

[0025] Please see Figure 2 , Figure 5 and Figure 6 In this embodiment, the magnetic suction assembly includes a first permanent magnet 31 fixedly installed on the bottom edge of the magnetic suction cap 3, an engagement groove 32 correspondingly opened on the upper edge of the bottle cap 2, and a second permanent magnet 33 fixedly installed at the bottom of the engagement groove 32. The first permanent magnet 31 and the engagement groove 32 correspond one-to-one. When the first permanent magnet 31 is inserted into the engagement groove 32, the first permanent magnet 31 and the second permanent magnet 33 attract each other, so that the magnetic suction cap 3 fits tightly against the bottle cap 2.

[0026] It should be noted that the design of the magnetic component fully considers the safety and durability of actual use scenarios. The permanent magnet is made of corrosion-resistant neodymium iron boron material and is epoxy coated to prevent oxidation failure in long-term sterilization or humid environments. The engagement groove 32 has a guide slope for easy assembly alignment. Multiple magnets are evenly distributed circumferentially to ensure uniform pressure distribution and avoid local warping that could lead to air leakage. This connection method eliminates the need for screws, clips, or adhesive processes, simplifying the structure, reducing manufacturing costs, and improving the user experience.

[0027] The working principle of the above embodiments is as follows: Inject the prepared culture medium into the glass bottle 1 to the appropriate volume. After inoculating the explants, tighten the bottle cap 2 onto the bottle mouth, ensuring the sealing rubber ring is pressed tightly and sealed. Then, place the air filter membrane 6 into the mounting groove 4, covering the ventilation hole 5. Next, align and place the magnetic cap 3 so that the first permanent magnet 31 is inserted into the engagement groove 32 and attracted and fixed with the second permanent magnet 33. According to the needs of the current culture stage, rotate the magnetic cap 3 to adjust the overlap between the eccentric hole 7 and the ventilation hole 5: Closed state: The eccentric hole 7 is completely offset from the ventilation hole 5, with almost no gas entering, suitable for transportation, temporary storage, or the early callus stage requiring low oxygen induction; Low and medium ventilation state: Partial overlap. It controls the inflow of a small amount of air, which is suitable for maintaining a moderate gas exchange during the propagation period. In the high-ventilation state, the two holes are basically aligned, with the largest ventilation area, ensuring a sufficient oxygen supply during the rooting period. Outside air enters through the eccentric hole 7, passes through the air filter membrane 6 and enters the bottle after filtration. The CO2 and water vapor enriched in the bottle are slowly discharged through the annularly distributed conical air outlet micro-holes 8, forming a virtuous cycle. Throughout the process, the user can monitor the ventilation status in real time by rotating the indicator mark 9 and the status scale to ensure consistent operation. When the filter membrane ages or becomes clogged, a new membrane can be replaced simply by removing the magnetic cap 3. There is no need to replace the entire cap, which is economical, environmentally friendly, efficient and safe.

[0028] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Without further limitations, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes said element.

[0029] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A plant tissue culture flask, characterized in that: The device includes a glass bottle body (1), a bottle cap (2) installed on the top of the glass bottle body (1), and a magnetic cap (3) fitted to the top of the bottle cap (2). The upper end face of the bottle cap (2) is provided with a mounting groove (4), and the bottom of the mounting groove (4) is provided with a vent hole (5). An air filter membrane (6) is provided on the inner side of the mounting groove (4). The air filter membrane (6) is used to filter microorganisms and particulate matter in the outside air. The upper end face of the magnetic cap (3) is provided with an eccentric hole (7), and the upper end face of the magnetic cap (3) is provided with an air outlet micro-hole (8) located outside the eccentric hole (7). A magnetic assembly is provided between the magnetic cap (3) and the bottle cap (2). The magnetic assembly is used for the detachable connection between the magnetic cap (3) and the bottle cap (2). By rotating the magnetic cap (3), the overlapping area between the eccentric hole (7) and the vent hole (5) changes, thereby adjusting the gas flow rate entering the bottle.

2. The plant tissue culture flask according to claim 1, characterized in that: The surface of the glass bottle body (1) is provided with a scale, the bottle cap (2) and the glass bottle body (1) are connected by a threaded seal, and a sealing rubber ring is provided on the top of the inner side of the bottle cap (2).

3. A plant tissue culture flask according to claim 1, characterized in that: The magnetic cap (3) has a rotation indicator (9) on its outer wall, and the bottle cap (2) has a corresponding status indicator on its outer wall. The status indicator includes ventilation status scales for closed, low, medium and high, which are used to intuitively display the current ventilation status.

4. A plant tissue culture flask according to claim 1, characterized in that: The air filter membrane (6) is a hydrophobic polytetrafluoroethylene microporous filter membrane with a pore size of 0.1 to 0.45 μm. The outer diameter of the air filter membrane (6) matches the mounting groove (4), is tightly embedded in the mounting groove (4), and covers the ventilation hole (5).

5. A plant tissue culture flask according to claim 1, characterized in that: The eccentric hole (7) is a fan-shaped through hole. The geometric center of the eccentric hole (7) is offset from the rotation center of the magnetic cover (3). When the magnetic cover (3) rotates around its central axis, the overlapping area of ​​the eccentric hole (7) and the vent hole (5) changes, forming multiple positions such as closed, low ventilation, medium ventilation and high ventilation.

6. A plant tissue culture flask according to claim 1, characterized in that: The exhaust micro-holes (8) are arranged in a ring array along the edge of the magnetic cover (3), and the exhaust micro-holes (8) are all cone-shaped structures with smaller tops and larger bottoms. The exhaust micro-holes (8) are used to assist in exhaust.

7. A plant tissue culture flask according to claim 1, characterized in that: The magnetic suction assembly includes a first permanent magnet (31) fixedly installed on the bottom edge of the magnetic suction cap (3), a meshing groove (32) correspondingly opened on the upper edge of the bottle cap (2), and a second permanent magnet (33) fixedly installed at the bottom of the meshing groove (32). The first permanent magnet (31) and the meshing groove (32) correspond one-to-one. When the first permanent magnet (31) is inserted into the meshing groove (32), the first permanent magnet (31) and the second permanent magnet (33) attract each other, so that the magnetic suction cap (3) fits tightly against the bottle cap (2).

8. A plant tissue culture flask according to claim 1, characterized in that: The magnetic cover (3) can be completely disassembled, and the mounting groove (4) is an open structure. The outer wall of the magnetic cover (3) is provided with anti-slip texture.

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