Biological technology human stem cell storage device

Abstract

The utility model relates to the field of biological science and technology, and disclose a kind of biological technology is used human stem cell storage device, it includes base, the top of base is fixedly connected with liquid nitrogen tank body, the top of liquid nitrogen tank body is rotatably connected with top cover, the inside rotation of base is connected with rotating rod, rotating rod's outside is fixed with worm wheel, the inside rotation of base is connected with the worm that is adapted to worm wheel, the outside of worm is extended to the fixed connection of hand wheel of base in one side, and the inside of liquid nitrogen tank body is extended to the fixed connection of rotating disc of rotating rod top;The utility model has realized the quick location of cryopreservation tube fixed, avoid the shaking of cryopreservation tube in the device running process, convenient to operate simultaneously, adopt the scheme of four storage barrels around arrangement, complete rotary discharge from one discharge hole, greatly reduce the heat exchange between internal low-temperature environment and outside, effectively keep the stability of storage temperature.

Description

Technical Field

[0001] This utility model belongs to the field of bioscience and technology, specifically a human stem cell storage device for biotechnology. Background Technology

[0002] In the field of biotechnology, the storage of human stem cells is of paramount importance. Stem cells are usually stored in tiny containers called cryovials, which are then placed in liquid nitrogen tanks for long-term preservation.

[0003] Existing storage containers are often inconvenient to fix and position, and are prone to shaking or tipping during transfer or transportation, which can lead to sample tube breakage or label confusion, causing irreversible loss of precious stem cell resources. When a specific sample needs to be retrieved, the operator needs to open the entire storage device and search for and retrieve the sample at room temperature. This not only causes drastic temperature fluctuations inside the device, affecting the activity of other samples, but also exposes stem cells to an adverse environment, increasing the risk of contamination. Utility Model Content

[0004] In view of the above situation and to overcome the shortcomings of the prior art, this utility model provides a human stem cell storage device for biotechnology, which effectively solves the problems mentioned in the background.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a human stem cell storage device for biotechnology, comprising a base, a liquid nitrogen tank fixedly connected to the top of the base, a top cover rotatably connected to the top of the liquid nitrogen tank, a rotating rod rotatably connected inside the base, a worm gear fixedly sleeved on the outside of the rotating rod, a worm adapted to the worm gear rotatably connected inside the base, one side of the worm extending through to the outside of the base and fixedly connected to a handwheel, a rotating disk extending above the rotating rod into the interior of the liquid nitrogen tank and fixedly connected to it, four placement cylinders arranged in a circular array fixedly connected above the rotating disk, cryopreservation tubes being installed inside the placement cylinders, positioning grooves being provided on both sides of the interior of the placement cylinders, a limit mechanism being provided above the cryopreservation tubes, a discharge hole being provided above the liquid nitrogen tank, a rectangular frame fixedly connected to the top of the liquid nitrogen tank, a cylinder fixedly connected to the inner wall of the rectangular frame, a sealing gasket fixedly connected to the output end of the cylinder, the sealing gasket being located above the discharge hole.

[0006] Preferably, the limiting mechanism includes a box body, which is fixedly installed above the cryopreservation tube. A limiting rod is fixedly connected inside the box body. Movable plates are movably sleeved on both sides of the limiting rod. A first spring is movably sleeved on the outside of the limiting rod. The two sides of the first spring are fixedly connected to the movable plates.

[0007] Preferably, a positioning rod is fixedly connected to the side of each of the two movable plates that are far apart. One side of the positioning rod extends through to the outside of the box body. The positioning rod is movably inserted into the positioning groove. A first wedge block is fixedly connected to the corresponding side of each of the two movable plates.

[0008] Preferably, a handle is fixedly connected to the top of the box body, a T-shaped rod is provided above the handle, a slot is provided on one side of the T-shaped rod, and the bottom of the T-shaped rod extends into the interior of the box body and is fixedly connected to a second wedge block, which is adapted to the first wedge blocks on both sides.

[0009] Preferably, a rectangular groove is provided on one side above the handle, a crossbar is fixedly connected inside the rectangular groove, a slide plate is movably sleeved on the outside of the crossbar, a second spring is fixedly connected to one side of the slide plate, the second spring is sleeved on the outside of the crossbar, and an insert rod is fixedly connected to one side of the slide plate, with one side of the insert rod movably inserted into the slot.

[0010] Compared with the prior art, the beneficial effects of this utility model are: 1. This utility model uses a sliding plate to move to one side, which moves the insertion rod away from the slot and compresses the second spring. When the insertion rod leaves the slot, the elastic force of the first spring pulls the moving plates on both sides closer together. The moving plates move the first wedge block and the positioning rod closer together. The first wedge block moves the matching second wedge block upward. When the positioning rod leaves the positioning groove, the handle is pulled upward, and the handle pulls the cryopreservation tube upward. When the cryopreservation tube is fixed, the T-shaped rod is pressed down and moves downward. The T-shaped rod moves the first wedge blocks on both sides away from each other through the second wedge block. The first wedge block moves the positioning rod into the positioning groove through the moving plate and stretches the first spring. At this time, the elastic force of the second spring will move the insertion rod into the slot, thus completing the limit and realizing the quick limit and fixation of the cryopreservation tube, preventing the cryopreservation tube from shaking during the operation of the device, and making disassembly and disassembly convenient. 2. This utility model uses the contraction of a cylinder to move the sealing gasket, opening the discharge hole. Then, the handwheel is rotated, which drives the worm gear to rotate. The worm gear drives the rotating rod to rotate, which drives the rotating disk to rotate. The rotating disk drives the placement cylinder to rotate. The cryogenic tubes can be taken out one by one through the discharge hole. The scheme of four storage cylinders arranged in a circle, and the rotation discharge is completed from one discharge hole, greatly reduces the heat exchange between the internal low temperature environment and the outside, and effectively maintains the stability of the storage temperature. Attached Figure Description

[0011] The accompanying drawings are provided to further understand the present invention and form part of the specification. They are used together with the embodiments of the present invention to explain the present invention and do not constitute a limitation thereof.

[0012] In the attached diagram: Figure 1 This is a schematic diagram of the overall structure of this utility model; Figure 2 This is a cross-sectional view of the structure of this utility model; Figure 3 This is a schematic diagram of the structure of the rotating disk of this utility model; Figure 4 This is a schematic diagram of the limiting mechanism of this utility model; Figure 5 This utility model Figure 4 Enlarged view of the structure of A in the middle; In the diagram: 1. Base; 2. Liquid nitrogen tank; 3. Top cover; 4. Worm gear; 5. Worm; 6. Handwheel; 7. Rotary disc; 8. Placement cylinder; 9. Cryopreservation tube; 10. Discharge port; 11. Rectangular frame; 12. Cylinder; 13. Sealing gasket; 14. Positioning groove; 15. Limiting mechanism; 1501. Box body; 1502. Limiting rod; 1503. Moving plate; 1504. Positioning rod; 1505. First wedge block; 1506. First spring; 1507. Second wedge block; 1508. Handle; 1509. Rectangular groove; 1510. Crossbar; 1511. Second spring; 1512. Slide plate; 1513. Insert rod; 1514. Slot; 1515. T-shaped rod; 16. Rotating rod. Detailed Implementation

[0013] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present utility model without creative effort are within the protection scope of the present utility model.

[0014] Example 1, by Figures 1-5The present invention includes a base 1, which provides stable support for the components above and protects the internal transmission components. A liquid nitrogen tank 2 is fixedly connected to the top of the base 1, and a top cover 3 is rotatably connected to the top of the liquid nitrogen tank 2. The top cover 3 is rotatably connected to the top of the liquid nitrogen tank 2 and can be opened and closed flexibly. A rotating rod 16 is rotatably connected inside the base 1, and a worm gear 4 is fixedly sleeved on the outside of the rotating rod 16. A worm 5 adapted to the worm gear 4 is rotatably connected inside the base 1. One side of the worm 5 extends through to the outside of the base 1 and is fixedly connected to a handwheel 6. A rotating disk 7 extends above the rotating rod 16 into the interior of the liquid nitrogen tank 2 and is fixedly connected to it. Four placement cylinders 8 arranged in a circular array are fixedly connected above the rotating disk 7. The placement cylinders 8 are equipped with cryopreservation containers. Cryopreservation tubes 9 are used to seal and store stem cells, preventing direct contact between stem cells and liquid nitrogen. Positioning grooves 14 are provided on both sides inside the placement cylinder 8. A limiting mechanism 15 is provided above the cryopreservation tube 9. A discharge hole 10 is provided above the liquid nitrogen tank 2. A rectangular frame 11 is fixedly connected to the top of the liquid nitrogen tank 2. A cylinder 12 is fixedly connected to the inner wall of the rectangular frame 11. A sealing gasket 13 is fixedly connected to the output end of the cylinder 12. The sealing gasket 13 is located above the discharge hole 10. When the rotating rod 16 drives the rotating disk 7 to rotate, it drives all the placement cylinders 8 to move smoothly, so that the cryopreservation tube 9 can be accurately aligned with the discharge hole 10. The cylinder 12 drives the sealing gasket 13 to move and open the discharge hole 10. After the placement is completed, the cylinder 12 pushes the sealing gasket 13 to move and fit the discharge hole 10, achieving rapid sealing.

[0015] The limiting mechanism 15 includes a box body 1501, which is fixedly installed above the cryopreservation tube 9. A limiting rod 1502 is fixedly connected inside the box body 1501. Movable plates 1503 are movably sleeved on both sides of the limiting rod 1502. A first spring 1506 is movably sleeved on the outside of the limiting rod 1502. Both sides of the first spring 1506 are fixedly connected to the movable plates 1503. A positioning rod 1504 is fixedly connected to the side of each movable plate 1503 that is furthest away from the other. One side of the positioning rod 1504 extends through to the outside of the box body 1501. The positioning rod 1504 is connected to the... The positioning slot 14 is movably inserted, and the limiting rod 1502 serves as a motion guide, restricting the moving plate 1503 to slide only in the horizontal direction. This prevents the moving plate 1503 from shifting, which would cause the positioning rod 1504 to fail to align with the positioning slot 14. When the first spring 1506 is subjected to external force, it pushes the moving plate 1503 to expand outward, keeping the positioning rod 1504 inserted into the positioning slot 14 and ensuring the cryopreservation tube 9 is stably fixed. A first wedge block 1505 is fixedly connected to one side of each of the two moving plates 1503. A handle 1508 is fixedly connected to the top of the box body 1501, and a [missing information - likely a design feature] is provided above the handle 1508. A T-shaped rod 1515 has a slot 1514 on one side. The lower part of the T-shaped rod 1515 extends into the interior of the housing 1501 and is fixedly connected to a second wedge block 1507. The second wedge block 1507 is adapted to the first wedge blocks 1505 on both sides. A rectangular groove 1509 is provided on one side above the handle 1508. A crossbar 1510 is fixedly connected inside the rectangular groove 1509. A sliding plate 1512 is movably fitted on the outside of the crossbar 1510. A second spring 1511 is fixedly connected to one side of the sliding plate 1512 and is fitted onto the crossbar. On the outside of 1510, a rod 1513 is fixedly connected to one side of the slide plate 1512. One side of the rod 1513 is movably inserted into the slot 1514. After pressing the T-shaped rod 1515 until the positioning rod 1504 is inserted into the positioning slot 14, the second spring 1511 will push the slide plate 1512, so that the rod 1513 is inserted into the slot 1514, fixing the position of the T-shaped rod 1515. Pulling the slide plate 1512 compresses the second spring 1511, and the rod 1513 is disengaged from the slot 1514. The T-shaped rod 1515 moves upward under the elastic force of the first spring 1506, thus canceling the restriction on the cryopreservation tube 9.

[0016] Working principle: In use, by opening the top cover 3, the cylinder 12 retracts, causing the sealing gasket 13 to move and opening the discharge hole 10. Then, the handwheel 6 is rotated, which drives the worm wheel 4 to rotate via the worm 5. The worm wheel 4 drives the rotating rod 16 to rotate, which in turn drives the rotating disk 7 to rotate. The rotating disk 7 then drives the placement cylinder 8 to rotate. The cryogenic tubes 9 can be taken out sequentially through the discharge hole 10. The sliding plate 1512 moves to one side, causing the insertion rod 1513 to move away from the slot 1514. At the same time, the second spring 1511 is compressed. After the insertion rod 1513 leaves the slot 1514, the elastic force of the first spring 1506 pulls the moving plates 1503 on both sides closer together. The moving plates 1503 drive the first wedge block. 1505 and positioning rod 1504 move closer to each other. The first wedge block 1505 drives the matching second wedge block 1507 to move upward. When the positioning rod 1504 moves away from the positioning groove 14, the handle 1508 is pulled upward. The handle 1508 drives the cryopreservation tube 9 to be taken out upward. When the cryopreservation tube 9 is fixed, the T-shaped rod 1515 is pressed and moved downward. The T-shaped rod 1515 drives the first wedge blocks 1505 on both sides to move away from each other through the second wedge block 1507. The first wedge block 1505 drives the positioning rod 1504 to insert into the positioning groove 14 through the moving plate 1503. At the same time, the first spring 1506 is stretched. At this time, the elastic force of the second spring 1511 will drive the insertion rod 1513 to insert into the slot 1514, thus completing the limit.

Claims

1. A biotechnology-based human stem cell storage device, comprising a base (1), characterized in that: A liquid nitrogen tank (2) is fixedly connected to the top of the base (1). A top cover (3) is rotatably connected to the top of the liquid nitrogen tank (2). A rotating rod (16) is rotatably connected inside the base (1). A worm gear (4) is fixedly sleeved on the outside of the rotating rod (16). A worm (5) adapted to the worm gear (4) is rotatably connected inside the base (1). One side of the worm (5) extends through to the outside of the base (1) and is fixedly connected to a handwheel (6). A rotating disk (7) extends above the rotating rod (16) into the inside of the liquid nitrogen tank (2) and is fixedly connected to the top of the rotating disk (7). Four ring array placement cylinders (8) are fixedly connected. A cryopreservation tube (9) is installed inside the placement cylinder (8). Positioning grooves (14) are opened on both sides inside the placement cylinder (8). A limit mechanism (15) is set above the cryopreservation tube (9). A discharge hole (10) is opened above the liquid nitrogen tank (2). A rectangular frame (11) is fixedly connected above the liquid nitrogen tank (2). A cylinder (12) is fixedly connected to the inner wall of the rectangular frame (11). A sealing gasket (13) is fixedly connected to the output end of the cylinder (12). The sealing gasket (13) is located above the discharge hole (10).

2. The biotechnology human stem cell storage device according to claim 1, characterized in that: The limiting mechanism (15) includes a box body (1501), which is fixedly installed above the cryopreservation tube (9). A limiting rod (1502) is fixedly connected inside the box body (1501). Movable plates (1503) are movably sleeved on both sides of the limiting rod (1502). A first spring (1506) is movably sleeved on the outside of the limiting rod (1502). The two sides of the first spring (1506) are fixedly connected to the movable plates (1503).

3. The biotechnology human stem cell storage device according to claim 2, characterized in that: A positioning rod (1504) is fixedly connected to one side of each of the two movable plates (1503) that are far apart. One side of the positioning rod (1504) extends through to the outside of the box (1501). The positioning rod (1504) is movably inserted into the positioning groove (14). A first wedge block (1505) is fixedly connected to one side of each of the two movable plates (1503).

4. A human stem cell storage device for biotechnology according to claim 2, characterized in that: A handle (1508) is fixedly connected to the top of the box body (1501). A T-shaped rod (1515) is provided above the handle (1508). A slot (1514) is provided on one side of the T-shaped rod (1515). The bottom of the T-shaped rod (1515) extends into the interior of the box body (1501) and is fixedly connected to a second wedge block (1507). The second wedge block (1507) is adapted to the first wedge blocks (1505) on both sides.

5. A human stem cell storage device for biotechnology according to claim 4, characterized in that: A rectangular groove (1509) is provided on one side above the grip (1508). A crossbar (1510) is fixedly connected inside the rectangular groove (1509). A sliding plate (1512) is movably sleeved on the outside of the crossbar (1510). A second spring (1511) is fixedly connected to one side of the sliding plate (1512). The second spring (1511) is sleeved on the outside of the crossbar (1510). A plug rod (1513) is fixedly connected to one side of the sliding plate (1512). One side of the plug rod (1513) is movably inserted into the slot (1514).

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