Solid phase extraction device suitable for batch samples

Abstract

The utility model provides a kind of solid phase extraction device suitable for batch sample belongs to extraction device technical field, including negative pressure box, the negative pressure box is placed in rack body;Sealing cover, the sealing cover is buckled on the negative pressure box, several through holes are opened on the sealing cover, the through hole side is provided with closure mechanism, for closing the through hole;Extraction column, extraction column one end inserts into the through hole, when extraction column is located in the through hole, the closure mechanism makes the through hole from closed state to unobstructed state;Sample collection cylinder, the sample collection cylinder is placed on the rack body, and the sample collection cylinder is correspondingly arranged with the extraction column;And negative pressure suction mechanism, the negative pressure suction mechanism is communicated with the negative pressure box inner cavity, for suctioning the air in the negative pressure box. Through the solid phase extraction device, multiple extraction column batch extraction operation can be realized, and in negative pressure environment, extraction efficiency can be accelerated.

Description

Technical Field

[0001] This utility model belongs to the field of extraction device technology, and specifically relates to a solid phase extraction device suitable for batch samples. Background Technology

[0002] Solid-phase extraction (SPE) is a sample pretreatment technique developed by combining liquid-solid extraction columns and liquid chromatography. It is primarily used for sample separation, purification, and concentration, effectively separating analytes from interfering components, reducing sample pretreatment steps, and offering simple, time-saving, and labor-saving operation. It is widely used in pharmaceuticals, food, environmental protection, commodity inspection, and chemical industries.

[0003] Most existing solid-phase extraction devices are negative pressure extraction devices, which typically have a negative pressure cavity with several extraction column connection holes above the cavity. The extraction column is inserted into the connection hole, and the sample inside the extraction column is drawn in by negative pressure suction, thereby achieving sample extraction.

[0004] However, due to the presence of several connection ports, when only some sample positions are processed, a large amount of negative pressure is wasted through unused connection ports, resulting in insufficient negative pressure for the actual sample positions, and a significantly reduced and uneven flow rate. Utility Model Content

[0005] Based on this, the present invention provides a solid phase extraction device suitable for batch samples to solve the technical problem in the prior art that, due to having several connection ports, when only a portion of the sample positions are processed, a large amount of negative pressure is wasted through unused connection ports, resulting in insufficient negative pressure for the actual sample positions, significantly reduced and uneven flow rate.

[0006] The technical solution of this utility model to solve the above-mentioned technical problems is as follows:

[0007] A solid-phase extraction apparatus suitable for batch samples, comprising:

[0008] A negative pressure chamber, wherein a shelf is provided inside the negative pressure chamber;

[0009] A sealing cover is fastened to the negative pressure box body. The sealing cover has several through holes, and a closing mechanism is provided on one side of each through hole to close the through hole.

[0010] An extraction column, one end of which is inserted into the connecting hole, and when the extraction column is located in the connecting hole, the sealing mechanism changes the connecting hole from a closed state to a free state;

[0011] A sample collection tube, wherein the sample collection tube is disposed on the placement frame and is correspondingly disposed to the extraction column; and

[0012] A negative pressure suction mechanism is connected to the inner cavity of the negative pressure chamber and is used to suction air from the inside of the negative pressure chamber.

[0013] Preferably, the sealing mechanism includes a torsion shaft and a sealing plate. The torsion shaft is disposed at the bottom of the sealing cover and can rotate under force. The sealing plate is connected to the torsion shaft and can fit against the through hole.

[0014] Preferably, a set of torsion shafts and a sealing plate are symmetrically arranged on both sides of the connecting hole.

[0015] Preferably, the sealing mechanism further includes a limiting plate and a return spring. A sliding groove is provided in the body of the sealing cover. The limiting plate is slidably disposed in the sliding groove, and one end of the limiting plate extends into the connecting hole. The return spring is disposed in the sliding groove, with one end connected to the limiting plate and the other end fixedly disposed in the sliding groove.

[0016] Preferably, the closing mechanism further includes a connecting rod, the cross-section of the limiting plate is inclined triangular, one end of the connecting rod is hinged to the closing plate, and the other end of the connecting rod extends into the sliding groove and contacts the inclined bottom surface of the limiting plate.

[0017] Preferably, the extraction column includes an extraction cylinder and a retaining sleeve. The extraction cylinder contains an extraction liquid. One end of the retaining sleeve is fitted onto the bottom of the extraction cylinder, and the other end of the retaining sleeve is fitted into the connecting hole. The retaining sleeve presses the limiting plate into the sliding groove and pushes open the sealing plate to the open state.

[0018] Preferably, the sealing cover has an L-shaped limiting groove, and the retaining sleeve has a limiting protrusion, which is engaged with the L-shaped limiting groove.

[0019] Preferably, the sealing cover has a sealing groove, which is engaged with the top of the negative pressure box.

[0020] Preferably, counterweights are also provided at the four corners of the sealing cover.

[0021] Compared with the prior art, the present invention has at least the following advantages:

[0022] This solid-phase extraction device enables batch extraction operations of multiple extraction columns, and can accelerate extraction efficiency under negative pressure. At the same time, due to the sealing mechanism of the sealing cover, the unused connecting holes are closed, and the negative pressure box can be kept in a stable negative pressure state during the suction process of the negative pressure suction mechanism. This not only improves the extraction efficiency of the extraction column, but also stabilizes the extraction rate. Attached Figure Description

[0023] Figure 1 Axonometric view of a solid-phase extraction apparatus suitable for batch samples.

[0024] Figure 2 This is a front view of a solid-phase extraction apparatus suitable for batch samples.

[0025] Figure 3 This is a top view of a solid-phase extraction apparatus suitable for batch samples.

[0026] Figure 4 Left view of a solid-phase extraction apparatus suitable for batch samples.

[0027] Figure 5 for Figure 4 AA sectional view.

[0028] Figure 6 for Figure 5 A magnified view of a section at point C.

[0029] Figure 7 This is the first axonometric view of the sealed cover.

[0030] Figure 8 for Figure 7 A magnified view of the area at point Z in the middle.

[0031] Figure 9 This is the second axonometric view of the sealing cover.

[0032] Figure 10 This is an isometric drawing of the caliper.

[0033] In the diagram: negative pressure box 100, placement frame 200, sealing cover 300, connecting hole 310, sliding groove 320, L-shaped limiting groove 330, sealing groove 340, counterweight 350, sealing mechanism 400, torsion shaft 410, sealing plate 420, limiting plate 430, return spring 440, connecting rod 450, extraction column 500, extraction cylinder 510, clamping cylinder 521, limiting protrusion 521, sample collection cylinder 600, negative pressure suction mechanism 700. Detailed Implementation

[0034] It should be noted that, unless otherwise specified, the embodiments and features described in these embodiments of the present invention can be combined with each other. The technical solutions of the present invention will be further described below with reference to the accompanying drawings of the embodiments. The present invention is not limited to the specific embodiments described below.

[0035] It should be understood that the same or similar reference numerals in the accompanying drawings of the embodiments correspond to the same or similar components. In the description of this utility model, it should be understood that if terms such as "upper," "lower," "front," "rear," "left," "right," "top," and "bottom" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, they are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or component referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, the terms describing positional relationships in the accompanying drawings are for illustrative purposes only and should not be construed as limiting this patent. Those skilled in the art can understand the specific meaning of the above terms according to the specific circumstances.

[0036] Please refer to Figures 1 to 10 A solid-phase extraction apparatus suitable for batch samples, comprising:

[0037] A negative pressure chamber 100 is provided, and a shelf 200 is provided inside the negative pressure chamber 100. The negative pressure chamber 100 is made of transparent glass, and the flow state of the extract liquid inside the negative pressure chamber 100 can be observed through the transparent glass.

[0038] A sealing cover 300 is fastened to the negative pressure chamber 100. When the sealing cover 300 is fastened to the top of the negative pressure chamber 100, the bottom plane of the sealing cover 300 and the top of the negative pressure chamber 100 are in close contact, forming a sealed space inside the negative pressure chamber 100. The sealing cover 300 has several through holes 310. A fixed number of through holes 310 are formed according to the surface area of ​​the sealing cover 300, generally 8-20 sets. When the through holes 310 are formed, the negative pressure chamber 100 is a non-sealed space. Therefore, a closing mechanism 400 is provided on one side of the through hole 310 to close the through hole 310. When the through hole 310 is sealed, a sealed space is formed inside the negative pressure chamber 100, which facilitates subsequent negative pressure suction.

[0039] Specifically, the closing mechanism 400 can be a closing plate 420 that independently closes each of the connecting holes 310.

[0040] Specifically, the sealing mechanism 400 can be a hinged sealing plate 420, which is disposed at the bottom of the sealing cover 300. Under normal conditions, the hinged sealing plate 420 blocks the through hole 310. After rotating the hinged sealing plate 420, the hinged sealing plate 420 does not block the through hole 310, and the through hole 310 is in an open state.

[0041] Specifically, the sealing mechanism 400 can be a magnetic sealing plate 420, which is set at the bottom of the sealing cover 300. Under normal conditions, the magnetic sealing plate 420 is magnetically attracted to the connecting hole 310, and the magnetic sealing plate 420 is pushed to move or rotate. The magnetic sealing plate 420 does not block the connecting hole 310, and the connecting hole 310 is in an open state.

[0042] Specifically, the placement frame 200 is provided with a number of positioning slots, which are corresponding to the connecting holes 310.

[0043] An extraction column 500 is inserted into the connecting hole 310 at one end. When the extraction column 500 is located in the connecting hole 310, the extraction column 500 pushes the sealing mechanism 400 to move or rotate, and the sealing mechanism 400 changes the connecting hole 310 from a closed state to a free state. At this time, the extraction column 500 is vertically sleeved in the connecting hole 310, and the liquid to be extracted in the extraction column 500 will flow into the negative pressure box 100.

[0044] A sample collection cylinder 600, which is a conventional transparent glass sample collection cylinder, is mounted on the placement frame 200. The placement frame 200 can hold several sample collection cylinders 600, and the sample collection cylinders 600 are correspondingly positioned to correspond with the extraction column 500. That is, when the extraction column 500 is vertically fitted into the connecting hole 310, the sample collection cylinder 600 is placed directly below the extraction column 500, and the extract in the extraction column 500 flows into the sample collection cylinder 600 below. It should be noted that the extraction column 500 can pass through the connecting hole 310 and be located directly above the opening of the sample collection cylinder 600, with a gap between the bottom end of the extraction column 500 and the opening of the sample collection cylinder 600; or, the extraction column 500 can extend through the connecting hole 310 into the sample collection cylinder 600, in which case the diameter of the opening of the sample collection cylinder 600 is larger than the diameter of the bottom end of the extraction column 500. In both of these ways, under negative pressure, it is convenient for the extract in the extraction column 500 to enter the sample collection cylinder 600.

[0045] The negative pressure suction mechanism 700 is an existing negative pressure device. The negative pressure suction mechanism 700 is connected to the inner cavity of the negative pressure box 100 and is used to suction the air inside the negative pressure box 100. Since the cavity inside the negative pressure box 100 is sealed, after the negative pressure suction mechanism 700 suctions the air inside the negative pressure box 100, it is only connected to the outside world through the extraction column 500. Under the action of pressure difference, the extract in the extraction column 500 is squeezed into the sample collection cylinder 600.

[0046] It should be noted again that although the cavity inside the negative pressure chamber 100 is a sealed cavity, even if there is a small amount of air leakage between the negative pressure chamber 100 and the sealed cover when the negative pressure suction mechanism 700 is in the suction state, the cavity inside the negative pressure chamber 100 is still a relatively sealed cavity, which can still create a pressure difference between the cavity inside the negative pressure chamber 100 and the external environment.

[0047] In one possible embodiment, see Figure 5 and Figure 6 The sealing mechanism 400 is a hinged sealing plate 420. The sealing mechanism 400 includes a torsion shaft 410 and a sealing plate 420. The torsion shaft 410 is located at the bottom of the sealing cover 300. The torsion shaft 410 can rotate under force. The sealing plate 420 is connected to the torsion shaft 410 and can fit against the through hole 310. When the extraction column 500 is not installed in the connecting hole 310, the torque shaft 410 is at the first rotation angle, driving the sealing plate 420 to rotate and fit against the bottom of the connecting hole 310, thus closing the connecting hole 310. When the extraction column 500 is fitted into the connecting hole 310, the extraction column 500 pushes the sealing plate 420 to flip, driving the torque shaft 410 to rotate to the second rotation angle, and the torque shaft 410 is under continuous force. When the extraction column 500 is pulled out of the connecting hole 310, the torque shaft 410 elastically resets, driving the sealing plate 420 to seal the connecting hole 310. This maintains the cavity of the negative pressure chamber 100 in a sealed state, facilitating negative pressure suction and maintaining negative pressure stability.

[0048] Specifically, a torsion spring is provided inside the torsion shaft 410. The tightening and loosening of the torsion spring drives the closed plate 420 to rotate. The torsion shaft 410 is an existing sleeve with a torsion spring. The specific structure of the torsion shaft 410 will not be described in detail here.

[0049] In a preferred embodiment, a set of torsion shafts 410 and a set of sealing plates 420 are symmetrically arranged on both sides of the through hole 310. When the extraction column 500 pushes the sealing plate 420, since there are two sets of sealing plates 420, the sealing plate 420 will open symmetrically on both sides.

[0050] In one possible embodiment, to further enhance the sealing effect within the negative pressure chamber 100, see [reference needed]. Figure 5 and Figure 6The closing mechanism 400 further includes a limiting plate 430 and a return spring 440. A sliding groove 320 is provided in the sealing cover 300. The limiting plate 430 is slidably disposed in the sliding groove 320. The limiting plate 430 and the sliding groove 320 are mutually engaged and sealed. One end of the limiting plate 430 extends into the connecting hole 310. The return spring 440 is disposed in the sliding groove 320. One end of the return spring 440 is connected to the limiting plate 430, and the other end is fixedly disposed in the sliding groove 320. Specifically, two sets of limiting plates 430 are provided, located on both sides of the connecting hole 310. The return spring 440 elastically extends, pressing the limiting plates 430 out into the connecting hole 310, aligning the two limiting plates 430 to seal the connecting hole 310. Simultaneously, a semi-circular groove is formed on each limiting plate 430, which, when aligned, forms a circular groove. The front end of the extraction column 500 extends into the groove and presses against the limiting plate. Plate 430 pushes the limiting plate 430 to slide within the sliding groove 320, causing the two limiting plates 430 to open. The limiting plate 430 compresses the corresponding return spring 440 to contract until the limiting plate 430 is completely retracted into the limiting groove. At the same time, the extraction column 500 pushes the sealing plate 420 to flip to both sides until the extraction column 500 extends into the negative pressure box 100. The limiting plate 430 also locks the extraction column 500 to prevent it from shaking within the connecting hole 310. After the extraction column 500 is pulled out from the connecting hole 310, the return spring 440 continues to push the limiting plate 430 back to slide in the sliding groove 320, so that the limiting plate 430 blocks the connecting hole; the limiting plate 430 blocks the connecting hole a second time, and cooperates with the sealing plate 420 to jointly block the connecting hole, so that the cavity in the negative pressure box 100 is in a closed state, enhancing the airtightness.

[0051] In one possible embodiment, see Figure 5 and Figure 6The sealing mechanism 400 also includes a connecting rod 450. The limiting plate 430 has an inclined triangular cross-section. One end of the connecting rod 450 is hinged to the sealing plate 420, and the other end of the connecting rod 450 extends into the sliding groove 320 and contacts the inclined bottom surface of the limiting plate 430. Specifically, a movable groove for the connecting rod 450 is provided in the sealing cover 300, and the connecting rod 450 can move up and down in the movable groove. When the extraction column 500 presses the limiting plate 430 to slide in the sliding groove 320, since the bottom surface of the limiting plate 430 is inclined, the inclined bottom surface of the limiting plate 430 will push the connecting rod 450 to slide downward in the vertical direction during the sliding process. Since the other end of the connecting rod 450 is hinged to the sealing plate 420, it will push the sealing plate 420 to flip, so that the connecting hole 310 is in an open state. By setting the linkage rod 450, the movement of the limiting plate 430 drives the opening of the closing plate 420 through the linkage rod 450, and the two have a linkage effect, which can simultaneously open or close the connecting hole 310.

[0052] During the installation of the sealing mechanism 400, the sealing cover 300 is divided into an upper cover and a lower cover. The sealing cover 300 is separated, and the lower cover is placed in a horizontal position. The torsion shaft 410, the sealing plate 420, the limiting plate 430, the return spring 440 and the connecting rod 450 are installed in the lower cover in sequence. After installation, the upper cover is fastened to the lower cover and fixed by bolt connection.

[0053] In one possible embodiment, see Figures 7 to 10 The extraction column 500 includes an extraction cylinder 510 and a retainer 520. The extraction cylinder 510 contains an extraction liquid. One end of the retainer 520 is fitted onto the bottom of the extraction cylinder 510, and the other end is fitted into the connecting hole 310. The retainer 520 presses the limiting plate 430 into the sliding groove 320 and pushes open the sealing plate 420 to the open state. The diameter of the retainer 520 matches the diameter of the connecting hole 310. The retainer 520 can be inserted into the connecting hole 310 and fixed. The side wall of the retainer 520 presses against the limiting plate 430 and retracts into the sliding groove 320. The return spring 440 is in a retracted state under force. The front end of the retainer 520 presses against the sealing plate 420 and flips it over. The torque shaft 410 is in a state of rotation under force.

[0054] In a preferred embodiment, the cartridge 520 is provided in conjunction with the extraction cartridge 510. Generally, the extraction column 500 only includes the extraction cartridge 510 and does not include the cartridge 520.

[0055] In a preferred embodiment, to prevent the retaining cylinder 520 from shaking within the connecting hole 310, an L-shaped limiting groove 330 is provided on the sealing cover 300, and a limiting protrusion 521 is provided on the retaining cylinder 520. The limiting protrusion 521 engages with the L-shaped limiting groove 330. When the limiting protrusion 521 is aligned with the L-shaped limiting groove 330, the retaining cylinder 520 is pushed into the connecting hole 310. When the limiting protrusion 521 reaches the bottom of the L-shaped limiting groove 330, the retaining cylinder 520 is rotated, causing the limiting protrusion 521 to engage with the L-shaped limiting groove 330, thereby fixing the retaining cylinder 520 within the connecting hole 310. When the extraction cylinder 510 is engaged with the retaining cylinder 520, shaking of the extraction cylinder 510 and the retaining cylinder 520 is prevented.

[0056] In one possible embodiment, see Figure 9 The sealing cover 300 has a sealing groove 340, which is engaged with the top of the negative pressure box 100. By engaging the negative pressure box 100 within the sealing groove 340, the sealing effect between the negative pressure box 100 and the sealing cover 300 is improved.

[0057] In a preferred embodiment, counterweights 350 are also provided at the four corners of the sealing cover 300, which can increase the sealing effect between the sealing cover 300 and the negative pressure box 100.

[0058] Specific work process:

[0059] Place the negative pressure chamber 100 horizontally, and place the placement rack 200 inside the negative pressure chamber 100. Determine the type or quantity of sample solution to be extracted, such as two extracts. Place the corresponding number of sample collection cylinders 600 on the placement rack 200, such as two sample collection cylinders 600. Cover the negative pressure chamber 100 with the sealing cover 300. Take the clamping cylinder 520 and insert it into the connecting hole 310 directly above the sample collection cylinder 600. When the clamping cylinder... When the cylinder 520 is located within the connecting hole 310, the limiting plate 430 retracts into the sliding groove 320, the return spring 440 is also in a retracted state, the connecting rod 450 moves vertically downward, pushing the closing plate 420 to rotate around the torsion shaft 410, and the pair of closing plates 420 are in an open state. The clamping cylinder 520 is located directly above the receiving cylinder 600. The remaining connecting holes 310 that do not engage with the clamping cylinder 520 are closed by the closing plate 420 and the limiting plate 430. The connecting hole 310 connects the negative pressure chamber 100 to the outside through the clamp 520. The extraction cylinder 510 is fitted onto the clamp 520. The liquid to be extracted is added dropwise into the extraction cylinder 510. The negative pressure suction mechanism 700 is activated to slowly suction the air inside the negative pressure chamber 100. As the air inside the negative pressure chamber 100 decreases, the external atmospheric pressure, under the action of the pressure difference, compresses the liquid to be extracted in the extraction cylinder 510. The extracted liquid is then collected in the receiving chamber. The extraction operation is completed within the sample cylinder 600. This solid-phase extraction device enables batch extraction operations of multiple extraction columns 500, and under negative pressure, it can accelerate the extraction efficiency. At the same time, since the sealing cover 300 is equipped with the sealing mechanism 400, the unused connecting holes 310 are in a closed state. During the suction process of the negative pressure suction mechanism 700, the negative pressure box 100 can be maintained in a stable negative pressure state, which not only improves the extraction efficiency of the extraction column 500, but also stabilizes the extraction rate.

[0060] Obviously, the above embodiments of this utility model are merely examples for clearly illustrating this utility model, and are not intended to limit the implementation of this utility model. Those skilled in the art can make other variations or modifications based on the above description. It is neither necessary nor possible to exhaustively list all possible implementations here. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of this utility model should be included within the protection scope of this utility model.

Claims

1. A solid-phase extraction apparatus suitable for batch samples, characterized in that, include A negative pressure chamber, wherein a shelf is provided inside the negative pressure chamber; A sealing cover is fastened to the negative pressure box body. The sealing cover has several through holes, and a closing mechanism is provided on one side of each through hole to close the through hole. An extraction column, one end of which is inserted into the connecting hole, and when the extraction column is located in the connecting hole, the sealing mechanism changes the connecting hole from a closed state to a free state; A sample collection tube, wherein the sample collection tube is disposed on the placement frame and is correspondingly disposed to the extraction column; and A negative pressure suction mechanism is connected to the inner cavity of the negative pressure chamber and is used to suction air from the inside of the negative pressure chamber.

2. The solid-phase extraction apparatus for batch samples as described in claim 1, characterized in that, The sealing mechanism includes a torsion shaft and a sealing plate. The torsion shaft is located at the bottom of the sealing cover and can rotate under force. The sealing plate is connected to the torsion shaft and can fit against the through hole.

3. The solid-phase extraction apparatus for batch samples as described in claim 2, characterized in that, A set of torsion shafts and a sealing plate are symmetrically arranged on both sides of the connecting hole.

4. The solid-phase extraction apparatus for batch samples as described in claim 2, characterized in that, The sealing mechanism also includes a limiting plate and a return spring. A sliding groove is provided in the body of the sealing cover. The limiting plate is slidably disposed in the sliding groove, and one end of the limiting plate extends into the connecting hole. The return spring is disposed in the sliding groove, with one end connected to the limiting plate and the other end fixedly disposed in the sliding groove.

5. The solid-phase extraction apparatus for batch samples as described in claim 4, characterized in that, The closing mechanism also includes a connecting rod. The cross-section of the limiting plate is inclined triangular. One end of the connecting rod is hinged to the closing plate, and the other end of the connecting rod extends into the sliding groove and contacts the inclined bottom surface of the limiting plate.

6. The solid-phase extraction apparatus for batch samples as described in claim 5, characterized in that, The extraction column includes an extraction cylinder and a retainer. The extraction cylinder contains an extraction liquid. One end of the retainer is fitted onto the bottom of the extraction cylinder, and the other end of the retainer is fitted into the connecting hole. The retainer presses the limiting plate into the sliding groove and pushes open the sealing plate to the open state.

7. The solid-phase extraction apparatus for batch samples as described in claim 6, characterized in that, The sealing cover is provided with an L-shaped limiting groove, and the retaining sleeve is provided with a limiting protrusion, which is engaged with the L-shaped limiting groove.

8. The solid-phase extraction apparatus for batch samples as described in claim 1, characterized in that, The sealing cover has a sealing groove, which is engaged with the top of the negative pressure box.

9. The solid-phase extraction apparatus for batch samples as described in claim 1, characterized in that, The sealing cover is also equipped with counterweights at its four corners.

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