A rapid adjustment range pipette gun required in streptavidin antibody production

Abstract

The utility model discloses a kind of required quick adjustment range of streptavidin antibody production, including coaxially arranged barrel, operating rod and pipetting nozzle. Operating rod is movably arranged on the central axis of barrel, and is equipped with limit sheet. Limiting assembly and measuring assembly are provided in the barrel. Limiting assembly includes limit button, upper hydraulic cylinder, lower hydraulic cylinder and limit ring, and the upper and lower hydraulic cylinders are connected by hose, to realize limiting. Measuring assembly includes read head, scale and microprocessor, for accurately measuring pipetting volume. The utility model can quickly adjust pipetting gun range, save operation time, reduce the risk of contamination, improve the safety of cell culture.

Description

Technical Field

[0001] This utility model belongs to the field of biotechnology and relates to a pipette, particularly a pipette for rapid volume adjustment in the production of streptavidin antibodies, aiming to solve the problems of efficiency and risk of bacterial contamination in sugar supplementation during cell culture. Background Technology

[0002] In the maintenance culture of cells for streptavidin antibody production, precise control of the cell growth environment is crucial. Typically, an appropriate amount of glucose solution needs to be added to each cell shake flask based on real-time measured glucose concentrations to maintain normal cell metabolism and growth. However, due to individual differences in cell growth status within each shake flask, the measured glucose concentrations also vary slightly, resulting in different glucose addition amounts required for each flask. The frequent and time-consuming adjustments to the volume range using traditional pipettes become a bottleneck in such glucose addition operations. Operators must repeatedly adjust pipette settings to meet the precise glucose addition requirements of different shake flasks, which not only reduces work efficiency but also prolongs operation time. More importantly, for ease of operation, the feed flasks and cell shake flasks are often left open, which greatly increases the risk of airborne microorganisms entering the culture system, seriously threatening the stability of cell culture and the reliability of experimental results. Utility Model Content

[0003] To address the aforementioned problems, this utility model provides a rapidly adjustable volume pipette required for streptavidin antibody production, comprising a coaxially arranged cylinder, an operating rod, and a pipette tip. The operating rod is movably positioned on the central axis of the cylinder, with one end extending out of the cylinder as a push-pull part and the other end extending into the pipette tip to control pipetting. The feature is that the operating rod is provided with a limiting plate, and the cylinder is provided with a limiting component and a measuring component. The limiting assembly includes a limiting button movably disposed along the side wall of the cylinder, an upper hydraulic cylinder and a lower hydraulic cylinder fixed to the inner wall of the cylinder and disposed opposite to each other, and a limiting ring sleeved on the operating rod; piston rods extending into the upper and lower hydraulic cylinders are provided on both sides of the limiting button, the upper and lower hydraulic cylinders are connected by a hose, one end of the hose is disposed inside the limiting button, and the flow of liquid in the hose is suppressed by the compression of the limiting button; when the limiting button is pressed to a specific position, the compression disappears and the hose is opened; the limiting ring is connected to the limiting button through a connecting rod; The measuring component includes a reading head disposed on a limiting ring and a scale disposed axially on the inner wall of the cylinder. The reading head moves on the scale with the limiting ring and its position on the scale is read by a microprocessor electrically connected to the scale.

[0004] Furthermore, the limit button includes an inner cylinder and an outer cylinder, both of which are provided with two through holes for the flexible hose to pass through. The inner cylinder is fitted inside the outer cylinder, and a spring is provided at the bottom that is connected to the inner wall of the outer cylinder.

[0005] Furthermore, the inner wall of the outer cylinder is provided with a limiting block to prevent the inner cylinder from detaching.

[0006] Furthermore, the side wall of the cylinder is provided with a limiting groove parallel to the axis, the outer cylinder is embedded in the limiting groove, and can move along the axis direction.

[0007] Furthermore, a protective shell is provided outside the limiting groove.

[0008] Furthermore, the operating lever includes a push rod and a pipetting rod, the top of the cylinder is threaded with a cover, a retaining ring is provided inside, a stop block is fixed on the push rod, and a spring connects the stop block and the retaining ring.

[0009] Furthermore, the end of the push rod is provided with a push cylinder, one end of the pipette rod is located inside the push cylinder, and the other end extends into the pipette tip.

[0010] Furthermore, a sealing ring is provided at one end of the pipette tip located on the cylinder.

[0011] Furthermore, a spring connects the pipette tip at one end of the cylinder to the pipette rod at the other end of the push cylinder.

[0012] In summary, the beneficial effects of this utility model are as follows: This invention addresses the problems of time-consuming volume adjustment and susceptibility to bacterial contamination in existing technologies by providing a rapidly adjustable volume pipette required for streptavidin antibody production. Through a limiting component, rapid volume adjustment is achieved, thereby significantly improving sugar supplementation efficiency and saving operation time.

[0013] The rapid range adjustment enabled by the limiting components shortens the open time of the feeding bottle and cell shaker, effectively reducing the risk of microbial contamination of the culture system and ensuring the stability and safety of cell culture.

[0014] The measuring component can accurately read the pipetting volume, and combined with the simplified operation procedure, this invention is easy to operate. Even inexperienced operators can quickly get started and achieve accurate sugar supplementation, ensuring the consistency and reliability of experimental results. Attached Figure Description

[0015] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0016] Figure 1 This is a schematic diagram of the structure of this utility model; Figure 2 This is a schematic diagram of a partial structure of the limiting component.

[0017] In the diagram: 1-Cylinder; 2-Operating lever; 3-Pipette; 4-Limit plate; 5-Limit assembly; 51-Limit button; 511-Piston rod; 512-Inner cylinder; 513-Outer cylinder; 52-Upper hydraulic cylinder; 53-Lower hydraulic cylinder; 54-Hose; 55-Limit ring; 6-Measuring assembly; 61-Reading head; 62-Scale; 63-Microprocessor; 64-Display screen; 11-Top cover; 12-Retaining ring; 21-Push rod; 211-Push cylinder; 212-Push handle; 22-Pipette rod; 23-Resistant block. Detailed Implementation

[0018] The following is in conjunction with the appendix Figure 1-2 The present invention will be described in further detail below. Example

[0019] This quick-adjusting pipette mainly consists of a cylinder 1, an operating lever 2, and a pipette tip 3. The cylinder 1 is the main structure of the pipette, containing various components. The operating lever 2 is coaxially mounted inside the cylinder 1 and can move along the axis of the cylinder 1. One end of the operating lever 2 extends out of the cylinder 1, forming a push-pull section for easy operation; the other end extends into the pipette tip 3 to control the aspiration and dispensing of liquid.

[0020] The operating lever 2 is equipped with a limiting plate 4, which cooperates with the limiting assembly 5 to achieve rapid adjustment of the measuring range. The limiting assembly 5 includes a limiting button 51, an upper hydraulic cylinder 52, a lower hydraulic cylinder 53, and a limiting ring 55. The limiting button 51 is movably arranged along the side wall of the cylinder 1 for easy operation. The upper hydraulic cylinder 52 and the lower hydraulic cylinder 53 are fixed to the inner wall of the cylinder 1 and are arranged opposite each other. The limiting ring 55 is sleeved on the operating lever 2 and connected to the limiting button 51 via a connecting rod. The limiting button 51 has piston rods 511 on both sides, which extend into the upper hydraulic cylinder 52 and the lower hydraulic cylinder 53 respectively. The upper hydraulic cylinder 52 and the lower hydraulic cylinder 53 are connected by a hose 54, one end of which is located inside the limiting button 51. The limit button 51 includes an inner cylinder 512 and an outer cylinder 512. The inner cylinder 512 is sleeved inside the outer cylinder 513, and a spring connected to the inner wall of the outer cylinder 513 is provided at the bottom to ensure the reset function of the limit button 51.

[0021] When the limit button 51 is not pressed, the outer wall of the inner cylinder 512 and the inner wall of the outer cylinder 512 pull on the hose 54 and compress it, reducing the internal space of the hose 54 and thus inhibiting the flow of liquid inside the hose. When the limit button 51 is pressed to a specific position, that is, when the four through holes provided on the inner cylinder 512 and the outer cylinder 513 for the hose 54 to pass through are on the same axis, the compression disappears, the hose 54 is open, and at this time, the liquid in the upper and lower hydraulic cylinders can flow, and the piston rod 511 can move, thereby realizing the adjustment of the position of the limit ring 55.

[0022] To ensure smoother flow of the hose 54 when the limit button 51 is pressed, an elastic component can be installed where the hose 54 enters the limit button 51. For example, a spring can be connected to one end of the hose, and the other end of the spring can be placed at the through hole of the outer cylinder 512. When the limit button 51 is pressed, the spring can help the hose 54 overcome the friction between the hose and the outer wall of the inner cylinder 512 and the inner wall of the outer cylinder 512, helping the hose 54 to straighten and thus improve its flow.

[0023] In addition, a protective shell is provided outside the limiting groove to prevent accidental damage to the limiting button 51. The inner wall of the outer cylinder 513 is also provided with a limiting block to prevent the inner cylinder 512 from disengaging, ensuring the reliability of the structure. The side wall of the cylinder 1 is provided with a limiting groove parallel to the axis, and the outer cylinder 513 is embedded in the limiting groove and can move along the axial direction.

[0024] The measuring component 6 is used for accurate measurement of pipetting volume. This component includes a reading head 61 mounted on a limiting ring 55 and a scale 62 mounted on the inner wall of the cylinder 1. The scale 62 is axially aligned with the cylinder 1, and the reading head 61 moves along the scale 62 with the limiting ring 55. The reading head 61 is electrically connected to the scale 62 and is also connected to a microprocessor 63. The microprocessor 63 can read the position of the reading head 61 on the scale 62, thereby accurately calculating the pipetting volume. A display screen 64 can also be mounted on the outer wall of the cylinder to display the pipetting volume calculated by the microprocessor 63.

[0025] In use, the operator first adjusts the position of the limit ring 55 using the limit button 51 to set the desired pipetting volume. Then, the operator pushes the operating lever 2, causing the pipette 22 to insert into the pipette tip 3 to aspirate the liquid. Finally, the operator pushes the operating lever 2 again to dispense the liquid. The microprocessor 63 can display the pipetting volume in real time, facilitating precise control by the operator.

[0026] To achieve precise pipetting operations, the operating lever 2 includes a push rod 21 and a pipetting rod 22. A top cover 11 is threaded onto the top of the cylinder 1, and a retaining ring 12 is provided inside. A stop block 23 is fixed to the push rod 21. Part of the stop block 23 is located inside the cylinder 1, and the other part is cylindrical and extends out from the center hole of the top cover 11. The stop block 23 has a hole along its central axis and is fitted onto the push rod 21. A spring is provided between the lower part of the stop block 23 inside the cylinder 1 and the retaining ring 12. A push cylinder 211 is provided at the end of the push rod 21. One end of the pipetting rod 22 is located inside the push cylinder 211, and the other end extends into the pipetting tip 3. A sealing ring is provided at one end of the pipetting tip 3 located in the cylinder 1 to ensure the liquid is sealed. Furthermore, a spring connects the end of the pipetting tip 3 located in the cylinder 1 to the end of the pipetting rod 22 located in the push cylinder 211.

[0027] When the push handle 212 at the top of the operating lever 2 is pressed, the stop block 23 remains stationary against the top cover 11 due to the spring action. At this time, the operating lever 2 is in the first position. When the push handle 212 contacts the cylindrical top of the stop block 23, the bottom of the pipette 22 moves close to the outlet of the pipette tip 3, representing the maximum pipetting volume of the pipette. When the push handle 212 is pressed further, the stop block 23 moves downward under the action of the push handle 212, and the spring below the stop block 23 is compressed. At this time, the operating lever 2 is in the second position, and the bottom of the pipette 22 continues to move towards the outlet of the pipette tip 3 (but this does not affect the liquid absorption volume of the pipette tip 3), scraping the liquid off the inner wall of the pipette tip 3.

[0028] The above description is merely a preferred embodiment of the present utility model and is not intended to limit the scope of protection of the present utility model. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the scope of protection of the present utility model.

[0029] The above are all preferred embodiments of this utility model, and are not intended to limit the scope of protection of this utility model. Therefore, all equivalent changes made to the structure, shape and principle of this utility model should be covered within the scope of protection of this utility model.

Claims

1. A rapidly adjustable volume pipette required for the production of streptavidin antibodies, comprising a cylinder (1), an operating lever (2), and a pipette tip (3) coaxially arranged, wherein the operating lever (2) is movably disposed on the central axis of the cylinder (1), one end extending out of the cylinder (1) as a push-pull part, and the other end extending into the pipette tip (3) to control pipetting, characterized in that: The operating lever (2) is provided with a limiting piece (4), and the cylinder (1) is provided with a limiting component (5) and a measuring component (6). The limiting assembly (5) includes a limiting button (51) movably disposed along the side wall of the cylinder (1), an upper hydraulic cylinder (52) and a lower hydraulic cylinder (53) fixed to the inner wall of the cylinder (1) and disposed opposite to each other, and a limiting ring (55) sleeved on the operating rod (2); the limiting button (51) is provided with piston rods (511) on both sides extending into the upper hydraulic cylinder (52) and the lower hydraulic cylinder (53), the upper hydraulic cylinder (52) and the lower hydraulic cylinder (53) are connected through a hose (54), one end of the hose (54) is disposed in the limiting button (51), and the flow of liquid in the hose is suppressed by the squeezing of the limiting button (51). When the limiting button (51) is pressed to a specific position, the squeezing disappears and the hose (54) is open; the limiting ring (55) is connected to the limiting button (51) through a connecting rod. The measuring component (6) includes a reading head (61) disposed on a limiting ring (55) and a scale (62) disposed axially on the inner wall of the cylinder (1). The reading head (61) moves on the scale (62) with the limiting ring (55) and its position on the scale (62) is read by a microprocessor (63) electrically connected to the scale (62).

2. The quick-adjustment pipette according to claim 1, characterized in that: The limit button (51) includes an inner cylinder (512) and an outer cylinder (513). Both the inner cylinder (512) and the outer cylinder (513) are provided with two through holes for the hose (54) to pass through. The inner cylinder (512) is sleeved inside the outer cylinder (513) and has a spring at the bottom that is connected to the inner wall of the outer cylinder (513).

3. The quick-adjustment pipette according to claim 2, characterized in that: The inner wall of the outer cylinder (513) is provided with a limiting block to prevent the inner cylinder (512) from detaching.

4. The quick-adjusting volumetric pipette according to claim 2, characterized in that: The side wall of the cylinder (1) is provided with a limiting groove parallel to the axis, and the outer cylinder (513) is embedded in the limiting groove and can move along the axis.

5. The quick-adjustment pipette according to claim 4, characterized in that: The limiting groove is provided with a protective shell.

6. The quick-adjustment pipette according to claim 1, characterized in that: The operating lever (2) includes a push rod (21) and a pipette (22). The top of the cylinder (1) is threaded with a cover (11) and a retaining ring (12) is provided inside. A stop block (23) is sleeved on the push rod (21) and a spring is connected between the stop block (23) and the retaining ring (12).

7. The quick-adjusting volumetric pipette according to claim 6, characterized in that: The push rod (21) has a push tube (211) at its end. One end of the pipette (22) is placed inside the push tube (211), and the other end extends into the pipette tip (3).

8. The quick-adjusting volumetric pipette according to claim 7, characterized in that: The pipette tip (3) is equipped with a sealing ring at one end of the cylinder (1).

9. The quick-adjustment pipette according to claim 7, characterized in that: A spring is connected to one end of the pipette tip (3) located in the cylinder (1) and the other end of the pipette rod (22) located in the push cylinder (211).

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