Pre-treatment system for microbial detection in water samples

Abstract

The utility model relates to laboratory automation technical field discloses a kind of microbial detection pretreatment systems in water sample, including in rack, sample unit, liquid processing unit and petri dish processing unit are set on the workbench of rack, the upper portion of rack is set to move liquid unit, sample unit includes sample holder and open-close lid device, open-close lid device is used to carry out open lid and lid action to sample container;Liquid processing unit includes test tube rack, suction head rack, vortex machine and test tube moving device, test tube moving device moves test tube between test tube rack and vortex machine;Petri dish processing unit includes petri dish open-close lid device, petri dish input-output device and culture medium injection device, petri dish open-close lid device is injected culture medium by culture medium injection device after petri dish is opened lid.The utility model integrates multiple modules such as water sample input, dilution processing and quantitative injection in petri dish to a system, realizes efficient sample microbial culture pretreatment work.

Description

Technical Field

[0001] This utility model relates to the field of laboratory automation technology, and in particular to a pretreatment system for detecting microorganisms in water samples. Background Technology

[0002] In water quality testing experiments, water samples are collected from the environment and sent back to the laboratory for testing of various parameters.

[0003] The testing process includes opening the water sample bag, taking a sample from the water sample bag using a dispensing gun, sending it to a test tube rack for gradient dilution, and then sending it to a petri dish for processing. The petri dish is fed and discharged by a dish input / output processing device. After input, culture medium is added, and then the diluted water sample is added to the petri dish. The sample is then spread, streaked, or shaken to ensure that the sample is evenly distributed on the culture medium.

[0004] Existing automated equipment is developed in individual modules. For example, the operation of opening and closing the water sample bag is a unit. When multiple modules work separately, personnel are needed to coordinate and handle the process in order to transfer materials in an orderly manner between modules, so that the testing process can be carried out in a cyclical manner.

[0005] The existing separate modules make it difficult to connect the workflow well and achieve efficient and rapid processing of multiple samples. Utility Model Content

[0006] The purpose of this invention is to solve the above-mentioned problems and provide a pretreatment system for microbial detection in water samples. This system integrates multiple modules such as water sample input, dilution treatment, and quantitative injection in petri dishes into one system, thereby achieving efficient pretreatment of microbial culture in samples.

[0007] The technical solution adopted by this utility model is: A pretreatment system for detecting microorganisms in water samples, characterized by comprising a frame, a sample unit, a liquid processing unit, and a petri dish processing unit arranged on the worktable of the frame, an X-axis track above the frame, a Y-axis track on the X-axis track, and a pipetting unit on the Y-axis track. The sample unit includes a sample rack and a cap-opening / closing device. The sample rack is used to arrange sample containers in an array, and the cap-opening / closing device is used to open and close the sample containers. The liquid processing unit includes a test tube rack, a pipette tip rack, a vortex mixer, and a test tube moving device. The test tube moving device moves the test tubes between the test tube rack and the vortex mixer. The petri dish processing unit includes a petri dish cap-opening / closing device, a petri dish input / output device, and a culture medium injection device. After the petri dish cap-opening / closing device opens the petri dish, the culture medium is injected by the culture medium injection device. The pipetting unit takes a sample from the sample unit and injects it into a test tube in the liquid processing unit. After dilution and vortexing, the sample is then transferred by the pipetting unit to a petri dish containing culture medium. Finally, the sample is output by the petri dish processing unit for microbial culture.

[0008] Furthermore, the sample unit includes a rotating frame and a sample placement position, with sample containers arranged in an array on the rotating frame.

[0009] Furthermore, the opening and closing device includes a column and a lifting and rotating clamp mounted on the column. The clamp holds the container lid on the rotating frame and rotates it to open and close the lid.

[0010] Furthermore, the test tube rack of the liquid processing unit is used to place the test tube array. The test tube rack is set on a moving track, and the movement of the test tube rack enables the test tube moving device to operate on the corresponding test tubes.

[0011] Furthermore, the test tube moving device is positioned across both sides of the test tube rack.

[0012] Furthermore, the sample unit and the liquid processing unit are arranged side by side, the petri dish processing unit is located in front of both, and the X-axis track is located on the top frame of the machine.

[0013] Furthermore, the culture dish opening and closing device of the culture dish processing unit is a turntable type, which separates the dish lid and the dish body by means of the upper and lower turntables, and the culture medium injection device injects the culture medium from the middle.

[0014] Furthermore, the culture dish input / output device includes a turntable rack, which corresponds to the culture dish opening and closing device, allowing the culture dish to be alternately input and output from the turntable rack and the culture dish opening and closing device.

[0015] Furthermore, the culture medium injection device of the petri dish processing unit includes a heat preservation device, in which the culture medium is placed and output by a peristaltic pump. The beneficial effects of this invention are: (1) Modular integration enables the pretreatment process of water sample microbial culture and detection to be streamlined, and a closed loop is formed between the modules through the liquid transfer unit of the robotic arm; (2) Packaged as a separate device, it takes up little space, is easy to operate, and is more efficient. Attached Figure Description

[0016] Appendix Figure 1 This is a schematic diagram of the overall structure of this utility model; Appendix Figure 2 This is a schematic diagram of the functional module layout structure of this utility model; Appendix Figure 3 This is a magnified view of a portion of the pipetting unit; Appendix Figure 4 This is a magnified view of a portion of the sample unit; Appendix Figure 5 This is a schematic diagram of the liquid processing unit; Appendix Figure 6 This is a magnified view of a portion of the petri dish processing unit; Appendix Figure 7 This is a schematic diagram of the insulation device.

[0017] The labels in the attached diagram are as follows: 1. Frame; 2. Workbench; 3. Sample handling unit; 4. Liquid handling unit; 5. Petri dish processing unit; 6. X-axis track; 7. Y-axis track; 8. Pipetting unit; 9. Sample rack; 10. Lid opening and closing device; 11. Column; 12. Lifting and rotating chuck; 13. Test tube rack; 14. Pipe tip rack; 15. Vortex generator; 16. Test tube moving device; 17. Moving track; 18. Petri dish opening and closing device; 19. Petri dish input / output device; 20. Culture medium injection device; 21. Turntable frame; 22. Insulation device; 23. Peristaltic pump; 24. Connecting pipe. Detailed Implementation

[0018] The specific implementation of the pretreatment system for microbial detection in water samples of this utility model will be described in detail below with reference to the accompanying drawings.

[0019] See appendix Figure 1The water sample microbial detection pretreatment system of this patent has a frame structure. The frame 1 is equipped with a workbench 2. Various functional units are arranged above the workbench 2. Electrical control components and driving components of the functional units are arranged below the workbench 2. It also includes some spare parts and consumables storage.

[0020] The functional units on workbench 2 include sample unit 3, liquid processing unit 4 and petri dish processing unit 5. The functional units complete multiple processes of water sample collection, dilution and dispensing.

[0021] See appendix Figure 2 The sample unit 3 and the liquid processing unit 4 are arranged side by side, and the petri dish processing unit 5 is located in front of them.

[0022] See appendix Figure 3 An X-axis track 6 is installed above the frame 1, located on the top frame of the frame 1. A Y-axis track 7 is installed on the X-axis track 6, and a pipetting unit 8 is installed on the Y-axis track 7. The pipetting unit 8 performs pipetting operations during the transfer and dilution of water samples between the functional units.

[0023] See appendix Figure 4 The sample unit 3 includes a sample rack 9 and a lid opening and closing device 10. The sample rack 9 is used to place sample containers in an array, and the lid opening and closing device 10 is used to open and close the sample containers. The sample unit 3 includes a rotating frame and a sample placement position, on which sample containers are arranged in an array. The lid opening and closing device 10 includes a column 11 and a lifting and rotating clamp 12 provided on the column 11. The clamp holds the container lid on the rotating frame and rotates it to open and close the lid.

[0024] See appendix Figure 5 The liquid processing unit 4 includes a test tube rack 13, a pipette tip holder 14, a vortex mixer 15, and a test tube moving device 16. The test tube moving device 16 moves the test tubes between the test tube rack 13 and the vortex mixer 15. The test tube rack 13 of the liquid processing unit 4 is used to hold the test tube array. The test tube rack 13 is set on a moving track 17. The movement of the test tube rack 13 causes the test tube moving device 16 to operate on the corresponding test tubes. The test tube moving device 16 is positioned across both sides of the test tube rack 13.

[0025] See appendix Figure 6 The petri dish processing unit 5 includes a petri dish opening and closing device 18, a petri dish input and output device 19, and a culture medium injection device 20. After the petri dish opening and closing device 18 opens the petri dish, the culture medium is injected by the culture medium injection device 20. The petri dish opening and closing device 18 of the petri dish processing unit 5 is a turntable type, which separates the petri dish lid and the petri dish body by the upper and lower turntables, and the culture medium injection device 20 injects the culture medium from the middle.

[0026] The petri dish input / output device 19 includes a turntable 21, which corresponds to the petri dish opening / closing device 18, and alternately inputs and outputs petri dishes from the turntable 21 and the petri dish opening / closing device 18.

[0027] See appendix Figure 7 The culture medium injection device 20 of the petri dish processing unit 5 includes a heat preservation device 22. The culture medium is placed in the heat preservation device 22. The heat preservation device 22 is connected to the culture medium injection device through a connecting pipe 24. The culture medium is output from the heat preservation box by a peristaltic pump 23.

[0028] The working process of this patent is as follows: after the pipetting unit 8 takes the sample from the sample unit 3, it is injected into the test tube of the liquid processing unit 4. After dilution and vortexing, the sample is then sent by the pipetting unit 8 to the culture dish containing the culture medium. Finally, the sample is output by the culture dish processing unit 5 for microbial culture.

[0029] This patent protects a technical solution that integrates multiple modules into a system. However, many existing technologies already provide similar or identical structures for these functional modules. For example, the sample holder in a sample unit holds the sample, and the lifting and rotating clamp opens and closes the lid. These existing technologies combine to achieve the opening and closing of the sample container. The structures and accessories of other modules can also be implemented using existing technologies.

[0030] The above are merely preferred embodiments of this utility model. It should be noted that those skilled in the art can make several improvements and modifications without departing from the principle of this utility model, and these improvements and modifications should also be considered within the scope of protection of this utility model.

Claims

1. A pretreatment system for detecting microorganisms in water samples, characterized in that: The system includes a frame, on which a sample unit, a liquid processing unit, and a petri dish processing unit are arranged. An X-axis track is positioned above the frame, and a Y-axis track is positioned on the X-axis track. A pipetting unit is positioned on the Y-axis track. The sample unit includes a sample rack and a capping device. The sample rack is used to arrange sample containers in an array, and the capping device is used to open and close the sample containers. The liquid processing unit includes a test tube rack, a pipette tip rack, a vortex mixer, and a test tube moving device. The test tube moving device moves the test tubes between the test tube rack and the vortex mixer. The petri dish processing unit includes a petri dish capping device, a petri dish input / output device, and a culture medium injection device. After the petri dish is opened by the capping device, the culture medium is injected by the culture medium injection device. The pipetting unit takes a sample from the sample unit and injects it into a test tube in the liquid processing unit. After dilution and vortexing, the sample is then transferred by the pipetting unit to a petri dish containing culture medium. Finally, the sample is output from the petri dish processing unit for microbial culture.

2. The pretreatment system for microbial detection in water samples according to claim 1, characterized in that: The sample unit includes a rotating frame and a sample placement position, with sample containers arranged in an array on the rotating frame.

3. The pretreatment system for microbial detection in water samples according to claim 2, characterized in that: The opening and closing device includes a column and a lifting and rotating clamp installed on the column. The clamp holds the container lid on the rotating frame and rotates it to open and close the lid.

4. The pretreatment system for microbial detection in water samples according to claim 1, characterized in that: The test tube rack of the liquid processing unit is used to place the test tube array. The test tube rack is set on a moving track. The movement of the test tube rack enables the test tube moving device to operate on the corresponding test tubes.

5. The pretreatment system for microbial detection in water samples according to claim 4, characterized in that: The test tube moving device is mounted across both sides of the test tube rack.

6. The pretreatment system for microbial detection in water samples according to claim 1, characterized in that: The sample unit and liquid processing unit are arranged side by side, the petri dish processing unit is located in front of both, and the X-axis track is located on the top frame of the machine.

7. The pretreatment system for microbial detection in water samples according to claim 1, characterized in that: The petri dish processing unit has a rotating lid opening and closing device that separates the petri dish lid and body by rotating the upper and lower plates, allowing the culture medium to be injected from the middle by the culture medium injection device.

8. The pretreatment system for microbial detection in water samples according to claim 7, characterized in that: The petri dish input / output device includes a turntable rack, which corresponds to the petri dish opening and closing device, allowing the petri dishes to be alternately input and output from the turntable rack and the petri dish opening and closing device.

9. The pretreatment system for microbial detection in water samples according to claim 8, characterized in that: The culture medium injection device of the petri dish processing unit includes a heat preservation device. The culture medium is placed in the heat preservation device and output by a peristaltic pump.

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