High-throughput real-time PCR reagent instrument

Abstract

The utility model discloses high -flux true expansion PCR reagent appearance, including bottom plate, the top of bottom plate is fixedly connected with workstation through support, the front end fixedly connected with reagent appearance body in the top of workstation, the top of workstation is provided with sampling board, the top of sampling board is equipped with sample hole. The utility model discloses the sampling board and sample hole of workstation top can concentrate and place multiple samples, cooperate the sample preservative in sample hole, can temporarily save the sample and avoid metamorphic, need not artificial one by one look after, the placing hole on the placing frame can fix eight connecting tubes, and the straight expansion reaction liquid is previously filled in eight connecting tubes, need not separate packing alone, adapts the demand of multiple samples reaction simultaneously, compared with traditional single -tube operation, and batch processing efficiency improves significantly, the sampling head of PCR tube cover bottom can accurate contact sample and reagent, avoids the dosage deviation of manual use pipette gun sampling, ensures that the reaction ratio of every sample and reagent is consistent, improves the accuracy of detection or experimental result.

Description

Technical Field

[0001] This utility model relates to the field of PCR reagent instrument technology, specifically a high-throughput true amplification PCR reagent instrument. Background Technology

[0002] High-throughput true amplification PCR reagent kits are core equipment for clinical pathogen detection (such as respiratory viruses and digestive tract pathogens) and scientific research gene amplification (such as gene mutation screening and gene expression quantification). These scenarios require the simultaneous processing of a large number of samples. However, the current high-throughput true amplification PCR reagent kits have low sample and reagent processing efficiency, requiring manual handling of sample tubes one by one and reagents to be individually aliquoted into single tubes. Batch processing is cumbersome and time-consuming. Therefore, we propose the high-throughput true amplification PCR reagent kit. Utility Model Content

[0003] The purpose of this invention is to provide a high-throughput true amplification PCR reagent instrument, which has the advantage of high sample and reagent processing efficiency. It solves the problems of low sample and reagent processing efficiency of current high-throughput true amplification PCR reagent instruments, the need for manual handling of sample tubes one by one, the need for reagents to be individually aliquoted into single tubes, and the cumbersome and time-consuming steps in batch processing.

[0004] To achieve the above objectives, this utility model provides the following technical solution: a high-throughput true amplification PCR reagent instrument, including a base plate, a worktable fixedly connected to the top of the base plate via a bracket, a reagent instrument body fixedly connected to the front end of the top of the worktable, a sampling plate provided on the top of the worktable, a sample hole opened on the top of the sampling plate, a placement rack fixedly connected to the rear end of the top of the worktable via a bracket, a placement hole opened on the placement rack, an eight-tube set inside the placement hole, a PCR tube cap provided on the top of the eight-tube set, and a sampling head provided at the bottom of the PCR tube cap.

[0005] Preferably, a cleaning box is placed on the top of the base plate, an ultrasonic cleaner is installed inside the cleaning box, a drain port is opened on the lower left side of the cleaning box, and a valve is installed inside the drain port.

[0006] Preferably, the inner cavity of the sample well is provided with a sample preservation solution, and the eight-tube connector is provided with a direct diffusion reaction solution.

[0007] Preferably, the bottom plate has fixing blocks fixedly connected to both the front and rear ends on the left and right sides, and bolts are provided on the fixing blocks.

[0008] Preferably, a toolbox is fixedly connected to the right end of the top of the base plate, and a partition is fixedly connected to the inner cavity of the toolbox.

[0009] Preferably, a battery box is fixedly connected to the left end of the top of the base plate, and a storage battery is fixedly connected to the inner cavity of the battery box.

[0010] Preferably, a PLC controller is fixedly connected to the right end of the top of the base plate, and the output terminal of the PLC controller is electrically connected to the input terminal of the ultrasonic cleaner.

[0011] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0012] 1. The sampling plate and sample wells on the top of the workbench of this utility model can hold multiple samples in one place. With the sample preservation solution in the sample wells, the samples can be temporarily preserved to prevent deterioration without manual inspection of each tube. The placement holes on the rack can fix eight tubes, which are pre-filled with direct amplification reaction solution. There is no need for individual dispensing, which can meet the needs of simultaneous reaction of multiple samples. Compared with traditional single-tube operation, the batch processing efficiency is significantly improved. The sampling head at the bottom of the PCR tube cap can accurately contact the sample and reagent, avoiding the dosage deviation of manual pipette sampling, ensuring that the reaction ratio of each sample and reagent is consistent, and improving the accuracy of detection or experimental results. The reagent instrument body at the front of the workbench provides stable temperature, time and other reaction conditions for gene amplification, ensuring the reliability of true amplification PCR reaction and reducing the result error caused by environmental fluctuations.

[0013] 2. The cleaning box on the top of the base plate and the ultrasonic cleaner inside the cavity of this utility model can efficiently clean the sampling head, eight-tube connector and other components after use. It uses ultrasonic vibration to remove residual samples, which is more thorough than manual cleaning and greatly reduces the risk of cross-contamination. The battery in the battery box at the top left of the base plate can automatically power the reagent instrument and ultrasonic cleaner in the event of a sudden power outage in the laboratory, ensuring that the current sample reaction or component cleaning process is completed and avoiding sample deterioration and reagent waste. Attached Figure Description

[0014] Figure 1 This is a schematic diagram of the structure of this utility model;

[0015] Figure 2 This is a schematic diagram of the rear view structure of this utility model;

[0016] Figure 3 This is a schematic diagram of the rear cross-sectional structure of this utility model;

[0017] Figure 4 This is a schematic diagram of the front sectional view of the present invention.

[0018] In the diagram: 1. Base plate; 2. Cleaning box; 3. PLC controller; 4. Toolbox; 5. Battery box; 6. Fixing block; 7. Bolt; 8. Workbench; 9. Reagent instrument body; 10. Sampling plate; 11. Sample hole; 12. Placement rack; 13. PCR tube cap; 14. Eight-tube strip; 15. Drain outlet; 16. Sampling head; 17. Placement hole; 18. Ultrasonic cleaner. Detailed Implementation

[0019] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0020] Secondly, the term "an embodiment" or "embodiment" as used herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The phrase "in one embodiment" appearing in different places in this specification does not necessarily refer to the same embodiment, nor is it a single or selective embodiment that excludes other embodiments.

[0021] Example 1:

[0022] Please see Figure 1-4 As shown, this utility model provides a high-throughput true amplification PCR reagent instrument, including a base plate 1. A workbench 8 is fixedly connected to the top of the base plate 1 via a bracket. The front end of the top of the workbench 8 is fixedly connected to the reagent instrument body 9. A sampling plate 10 is provided on the top of the workbench 8. A sample well 11 is opened on the top of the sampling plate 10. A placement rack 12 is fixedly connected to the rear end of the top of the workbench 8 via a bracket. A placement hole 17 is opened on the placement rack 12. An eight-tube bundle 14 is placed in the inner cavity of the placement hole 17. A PCR tube cap 13 is provided on the top of the eight-tube bundle 14. A sampling head 16 is provided at the bottom of the PCR tube cap 13. Sample preservation solution is provided in the inner cavity of the sample well 11. Direct amplification reaction solution is provided in the eight-tube bundle 14.

[0023] The sampling plate 10 and sample wells 11 on the top of the workbench 8 in this technical solution can hold multiple samples in one place. With the sample preservation solution in the sample wells 11, the samples can be temporarily preserved to prevent deterioration without the need for manual inspection of each tube. The placement holes 17 on the placement rack 12 can fix eight-tube bundles 14. The eight-tube bundles 14 are pre-filled with direct amplification reaction solution, eliminating the need for individual dispensing and adapting to the needs of simultaneous reaction of multiple samples. Compared with traditional single-tube operation, the batch processing efficiency is significantly improved. The sampling head 16 at the bottom of the PCR tube cap 13 can accurately contact the sample and reagent, avoiding the dosage deviation caused by manual pipetting and ensuring that the reaction ratio of each sample and reagent is consistent, thus improving the accuracy of detection or experimental results. The reagent instrument body 9 at the front of the workbench 8 provides stable reaction conditions such as temperature and time for gene amplification, ensuring the reliability of the true amplification PCR reaction and reducing the result error caused by environmental fluctuations.

[0024] Example 2:

[0025] Based on Embodiment 1, this utility model is as follows: Figure 1-4As shown, a cleaning box 2 is placed on the top of the base plate 1. An ultrasonic cleaner 18 is installed inside the cleaning box 2. A drain port 15 is opened on the lower left side of the inner cavity of the cleaning box 2. A valve is installed inside the drain port 15. Fixing blocks 6 are fixedly connected to the front and rear ends of both sides of the base plate 1. Bolts 7 are provided on the fixing blocks 6. A toolbox 4 is fixedly connected to the right end of the top of the base plate 1. A partition is fixedly connected to the inner cavity of the toolbox 4. A battery box 5 is fixedly connected to the left end of the top of the base plate 1. A storage battery is fixedly connected to the inner cavity of the battery box 5. A PLC controller 3 is fixedly connected to the right end of the top of the base plate 1. The output terminal of the PLC controller 3 is electrically connected to the input terminal of the ultrasonic cleaner 18.

[0026] The cleaning box 2 on the top of the base plate 1 and the ultrasonic cleaner 18 inside the cavity of this technical solution can efficiently clean the sampling head 16, the eight-tube connector 14 and other components after use. The ultrasonic vibration removes residual samples, which is more thorough than manual cleaning and greatly reduces the risk of cross-contamination. The battery in the battery box 5 at the top left of the base plate 1 can automatically power the reagent instrument body 9 and the ultrasonic cleaner 18 in the event of a sudden power outage in the laboratory, ensuring that the current sample reaction or component cleaning process is completed and avoiding sample deterioration and reagent waste.

[0027] The working principle of this utility model is as follows: The sampling plate 10 and sample wells 11 on the top of the workbench 8 can hold multiple samples together. Combined with the sample preservation solution in the sample wells 11, the samples can be temporarily preserved to prevent deterioration, eliminating the need for manual inspection of each tube. The placement holes 17 on the rack 12 can fix eight-tube bundles 14, which are pre-filled with direct amplification reaction solution, eliminating the need for individual dispensing and adapting to the simultaneous reaction of multiple samples. Compared to traditional single-tube operation, batch processing efficiency is significantly improved. The sampling head 16 at the bottom of the PCR tube cap 13 can accurately contact the sample and reagent, avoiding dosage deviations caused by manual pipetting, ensuring consistent reaction ratios for each sample and reagent, and improving the accuracy of detection or experimental results. The reagent instrument body 9 at the front end of the workbench 8 provides stable reaction conditions such as temperature and time for gene amplification, ensuring the reliability of true amplified PCR reactions and reducing result errors caused by environmental fluctuations. The cleaning box 2 at the top of the base plate 1 and the ultrasonic cleaner 18 in the inner cavity can efficiently clean the sample head 16, eight-tube connector 14 and other components after use. The ultrasonic vibration removes residual samples, which is more thorough than manual cleaning and greatly reduces the risk of cross-contamination. The battery in the battery box 5 at the top left of the base plate 1 can automatically power the reagent instrument body 9 and ultrasonic cleaner 18 in the event of a sudden power outage in the laboratory, ensuring that the current sample reaction or component cleaning process is completed and avoiding sample deterioration and reagent waste.

[0028] It is important to note that the constructions and arrangements of this application shown in several different exemplary embodiments are merely illustrative. Although only a few embodiments are described in detail in this disclosure, those who consult this disclosure will readily understand that many modifications are possible (e.g., changes in the size, dimensions, structure, shape and proportion of various elements, as well as parameter values ​​(e.g., temperature, pressure, etc.), mounting arrangements, use of materials, color, orientation, etc.) without substantially departing from the novel teachings and advantages of the subject matter described in this application). For example, an element shown as integrally formed may be composed of multiple parts or elements, the position of elements may be inverted or otherwise altered, and the nature or number or position of discrete elements may be changed or altered. Therefore, all such modifications are intended to be included within the scope of this utility model. The order or sequence of any process or method steps may be changed or reordered according to alternative embodiments. In the claims, any "device plus function" clause is intended to cover the structure described herein that performs the function, and not only structural equivalents but also equivalent structures. Without departing from the scope of this invention, other substitutions, modifications, alterations, and omissions may be made in the design, operation, and arrangement of the exemplary embodiments. Therefore, this invention is not limited to the specific embodiments, but extends to various modifications that still fall within the scope of the appended claims.

[0029] Furthermore, in order to provide a concise description of exemplary embodiments, not all features of actual embodiments (i.e., those features that are not relevant to the best mode of carrying out the present invention as currently considered, or those features that are not relevant to implementing the present invention) may be omitted.

[0030] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of this utility model, and are not intended to limit the scope of protection of this utility model. Although this utility model has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of this utility model without departing from the essence and scope of the technical solutions of this utility model.

Claims

1. A high-throughput true amplification PCR reagent instrument, including a base plate (1), characterized in that: The top of the base plate (1) is fixedly connected to a workbench (8) via a bracket. The front end of the top of the workbench (8) is fixedly connected to a reagent instrument body (9). A sampling plate (10) is provided on the top of the workbench (8). A sample hole (11) is opened on the top of the sampling plate (10). A placement rack (12) is fixedly connected to the rear end of the top of the workbench (8) via a bracket. A placement hole (17) is opened on the placement rack (12). An eight-tube (14) is placed in the inner cavity of the placement hole (17). A PCR tube cap (13) is provided on the top of the eight-tube (14). A sampling head (16) is provided at the bottom of the PCR tube cap (13).

2. The high-throughput true amplification PCR reagent instrument according to claim 1, characterized in that: A cleaning box (2) is placed on the top of the base plate (1). An ultrasonic cleaner (18) is installed in the inner cavity of the cleaning box (2). A drain port (15) is opened on the lower left side of the inner cavity of the cleaning box (2). A valve is installed in the inner cavity of the drain port (15).

3. The high-throughput true amplification PCR reagent instrument according to claim 1, characterized in that: The sample well (11) is filled with a sample preservation solution, and the eight-tube (14) is filled with a direct diffusion reaction solution.

4. The high-throughput true amplification PCR reagent instrument according to claim 1, characterized in that: The base plate (1) has fixed blocks (6) at both the front and rear ends on both sides, and bolts (7) are provided on the fixed blocks (6).

5. The high-throughput true amplification PCR reagent instrument according to claim 1, characterized in that: A toolbox (4) is fixedly connected to the right end of the top of the base plate (1), and a partition is fixedly connected to the inner cavity of the toolbox (4).

6. The high-throughput true amplification PCR reagent instrument according to claim 1, characterized in that: A battery box (5) is fixedly connected to the left end of the top of the base plate (1), and a storage battery is fixedly connected to the inner cavity of the battery box (5).

7. The high-throughput true amplification PCR reagent instrument according to claim 1, characterized in that: A PLC controller (3) is fixedly connected to the right end of the top of the base plate (1), and the output end of the PLC controller (3) is electrically connected to the input end of the ultrasonic cleaner (18).

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