Nucleic acid extraction kit

Abstract

This utility model discloses a nucleic acid extraction kit, relating to the field of kit technology. The kit includes a box body with a connecting seat at its upper end and a top cover at its upper end. An extraction chamber is located inside the box body, and a separating component is located inside the extraction chamber, with the separating component being pluggably connected to the box body. Multiple pressing notches are provided on the top surface of the connecting seat. A first baffle is provided on the inner wall of the extraction chamber, and multiple second baffles are provided on one side of the first baffle. A connecting slot is formed between the first and second baffles. This solution solves the problem in existing kits where the tweezers need to be broken during nucleic acid extraction, the breaking angle is large, it is difficult to break, and it is time-consuming and laborious, affecting extraction efficiency.

Description

Technical Field

[0001] This utility model relates to the field of reagent kit technology, specifically to a nucleic acid extraction reagent kit. Background Technology

[0002] Nucleic acid extraction kits are chemical kits used to extract DNA or RNA from biological samples and are widely used in fields such as molecular biology, genomics, and clinical diagnostics.

[0003] For example, the Chinese authorized patent (nucleic acid extraction kit) with publication number CN216764891U includes a box body. The box body contains a lysis chamber, an adsorption chamber with adsorbent material, a pipetting chamber, a gas chamber, and a valve. The pipetting chamber can be connected to one of the lysis chamber or the adsorption chamber via different rotation angles of the valve to transfer the liquefied and lysed sample from the lysis chamber to the adsorption chamber. The gas chamber allows the purified liquid from the adsorption chamber to be discharged through a filter membrane. This invention, by dividing the box body into two independent chambers, performs the lysis step in one chamber and the purification step in the other. This allows the nucleic acid extraction kit to be used in nucleic acid extraction processes, especially for nucleic acid extraction from infectious pathogen samples, effectively avoiding cross-contamination and personnel infection, while reducing extraction steps and improving extraction efficiency.

[0004] However, existing kits require the twisted strands to be broken during nucleic acid extraction. The breakage angle is large, making it difficult to break, which is time-consuming and laborious, affecting extraction efficiency. Therefore, they do not meet the current needs. To address this, we have proposed a nucleic acid extraction kit. Utility Model Content

[0005] The purpose of this invention is to provide a nucleic acid extraction kit to solve the problem mentioned in the background art that when performing nucleic acid extraction, the tweezers need to be broken at a large angle, which is difficult to break, time-consuming, labor-intensive, and affects the extraction efficiency.

[0006] To achieve the above objectives, this utility model provides the following technical solution: a nucleic acid extraction kit, comprising: a box body, a connecting seat at the upper end of the box body, a top cover at the upper end of the connecting seat, an extraction chamber inside the box body, a separating component inside the extraction chamber, and the separating component being pluggably connected to the box body; and a pressing notch on the top surface of the connecting seat, wherein multiple pressing notches are provided.

[0007] Preferably, the inner wall of the extraction cavity is provided with a first baffle, a second baffle is provided on one side of the first baffle, and multiple first and second baffles are provided, forming a connecting slot between the first and second baffles.

[0008] Preferably, the partition assembly includes a first partition plate and a second partition plate, which intersect to form a cross shape, and both the first partition plate and the second partition plate are inserted along the connecting slot.

[0009] Preferably, the bottom ends of the first and second partition plates are provided with notches, and there is a cavity between the notches and the bottom surface of the extraction cavity.

[0010] Preferably, the outer surface of the connecting seat is provided with an external thread, and the connecting seat is threadedly connected to the top cover through the external thread.

[0011] Preferably, the first and second partition plates divide the extraction cavity into four equal parts, and each of the four equal parts corresponds to the first downward pressure notch.

[0012] Compared with the prior art, the beneficial effects of this utility model are:

[0013] (1) This utility model sets a separator component inside the extraction cavity. The separator component consists of a first separator plate and a second separator plate. The separator component is inserted into the extraction cavity and the extraction cavity is divided into four equal parts by the first separator plate and the second separator plate. The four equal parts correspond to the first pressing notch. The bottom ends of the first separator plate and the second separator plate are provided with notches. There is a cavity between the notch and the bottom surface of the extraction cavity, which avoids bottom sealing and makes the four equal parts interconnected. This solves the problem that when the existing kit is used for nucleic acid extraction, the twist needs to be broken. The breaking angle is large and it is not easy to break. It is time-consuming and laborious and affects the extraction efficiency.

[0014] (2) By setting a first baffle and a second baffle on the inner wall of the extraction cavity, a connecting slot is formed between the first baffle and the second baffle. When inserted, the first baffle and the second baffle are inserted along the connecting slot, which has a limiting effect, improves stability, and avoids positional deviation. Attached Figure Description

[0015] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0016] Figure 2 This is a schematic diagram of the structure after the box body and top cover of this utility model are connected;

[0017] Figure 3 This is a schematic diagram of the internal structure of the box body of this utility model;

[0018] Figure 4 This is a schematic diagram of the disassembled structure of the box body and the partition component of this utility model;

[0019] In the diagram: 1. Box body; 2. Top cover; 3. Connecting seat; 4. External thread; 5. Extraction chamber; 6. Pressing notch; 7. Separating assembly; 8. First baffle; 9. Second baffle; 10. Connecting slot; 11. First partition plate; 12. Second partition plate; 13. Notch. Detailed Implementation

[0020] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments.

[0021] Please see Figure 1-4 This utility model provides an embodiment of a nucleic acid extraction kit, comprising: a box body 1, a connecting seat 3 at the upper end of the box body 1, a top cover 2 at the upper end of the connecting seat 3, an extraction chamber 5 inside the box body 1, a separating component 7 inside the extraction chamber 5, and the separating component 7 being pluggably connected to the box body 1; a pressing notch 6 on the top surface of the connecting seat 3, and multiple pressing notches 6; a first baffle 8 on the inner wall of the extraction chamber 5, and a second baffle 9 on one side of the first baffle 8, and multiple first baffles 8 and second baffles 9. A connecting slot 10 is formed between the plate 8 and the second baffle 9. The separating component 7 includes a first separating plate 11 and a second separating plate 12, which intersect to form a cross shape. Both the first separating plate 11 and the second separating plate 12 are inserted along the connecting slot 10. The bottom ends of the first separating plate 11 and the second separating plate 12 are provided with notches 13, and there is a cavity between the notches 13 and the bottom surface of the extraction cavity 5. The first separating plate 11 and the second separating plate 12 divide the extraction cavity 5 into four equal parts, and the four equal parts correspond to the first pressing notch 6 respectively.

[0022] The partition assembly 7, consisting of the first partition plate 11 and the second partition plate 12, is inserted into the extraction cavity 5. During insertion, both the first partition plate 11 and the second partition plate 12 are inserted along the connecting slot 10, which serves as a limiting effect, improves stability, and prevents positional displacement. The extraction cavity 5 is divided into four equal parts by the first partition plate 11 and the second partition plate 12, and the four equal parts correspond to the first pressing notch 6. The bottom ends of the first partition plate 11 and the second partition plate 12 are provided with notches 13, and there is a cavity between the notches 13 and the bottom surface of the extraction cavity 5 to prevent the bottom from being sealed and to allow the four equal parts to communicate with each other.

[0023] During nucleic acid extraction, the extraction end of the twist is inserted into one of the four equal parts of the space, and the twist is bent so that the bent position is against the pressing notch 6. The extraction end is located at the junction of the first partition plate 11 and the second partition plate 12, which shortens the original bending angle of the twist and increases the degree of breakage when the twist is bent. This makes it easier to break the twist when it is bent, thereby improving the extraction efficiency.

[0024] The outer surface of the connector 3 is provided with an external thread 4. The connector 3 is threaded to the top cover 2 through the external thread 4. After extraction, the top cover is closed, and the connector 3 is threaded to the top cover 2 through the external thread 4, thereby achieving a seal, so that it can be stored and carried.

[0025] It will be apparent to those skilled in the art that this invention is not limited to the details of the exemplary embodiments described above, and that it can be implemented in other specific forms without departing from the spirit or essential characteristics of this invention. Therefore, the embodiments should be considered illustrative and non-limiting in all respects, and the scope of this invention is defined by the appended claims rather than the foregoing description. Thus, it is intended that all variations falling within the meaning and scope of equivalents of the claims be included within this invention. No reference numerals in the claims should be construed as limiting the scope of the claims.

Claims

1. A nucleic acid extraction kit, comprising a housing (1), characterized in that: The upper end of the box body (1) is provided with a connecting seat (3), the upper end of the connecting seat (3) is provided with a top cover (2), the inside of the box body (1) is provided with an extraction cavity (5), the inside of the extraction cavity (5) is provided with a separating component (7), and the separating component (7) is plugged into the box body 1. The top surface of the connecting seat (3) is provided with a pressing notch (6), and there are multiple pressing notches (6).

2. The nucleic acid extraction kit according to claim 1, characterized in that: The inner wall of the extraction cavity (5) is provided with a first baffle (8), and a second baffle (9) is provided on one side of the first baffle (8). There are multiple first baffles (8) and second baffles (9), and a connecting slot (10) is formed between the first baffle (8) and the second baffle (9).

3. The nucleic acid extraction kit according to claim 2, characterized in that: The partition assembly (7) includes a first partition plate (11) and a second partition plate (12), and the first partition plate (11) and the second partition plate (12) intersect in a cross shape, and both the first partition plate (11) and the second partition plate (12) are inserted along the connecting slot (10).

4. The nucleic acid extraction kit according to claim 3, characterized in that: The bottom ends of the first partition plate (11) and the second partition plate (12) are provided with notches (13), and there is a cavity between the notches (13) and the bottom surface of the extraction cavity (5).

5. The nucleic acid extraction kit according to claim 1, characterized in that: The outer surface of the connecting seat (3) is provided with an external thread (4), and the connecting seat (3) is threadedly connected to the top cover (2) through the external thread (4).

6. The nucleic acid extraction kit according to claim 3, characterized in that: The first partition plate (11) and the second partition plate (12) divide the extraction cavity (5) into four equal parts, and the four equal parts correspond to the first pressing notch (6).

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