Human blood stem cell separation device

Abstract

The utility model relates to cell separation technical field, and disclose a human blood stem cell separation device, it includes the fixed disc, the inside movable mounting of fixed disc has the locating seat, the bottom surface fixed connection of locating seat has the connecting column, the bottom end rotation of connecting column extends to the below fixed connection of locating seat and drives the bevel gear, the inside movable mounting of locating seat has a plurality of test tubes, the bottom surface fixed mounting of locating seat has the protection box, the utility model discloses, through the second spring drive fixed plate and position test tube, again promote L shape piece and slide in the sliding slot, L shape piece inserts into the fixed block and uses bolt to fix, thereby L shape piece drives the sliding of toothed plate, toothed plate drives the rotation of locking gear, locking gear drives the rotation of cam further and pushes the fixed plate tightly fixed, and the multiple test tubes are clamped and are separated and handled conveniently, compared with each processing and accelerate the work progress.

Description

Technical Field

[0001] This invention belongs to the field of cell separation technology, specifically a human blood stem cell separation device. Background Technology

[0002] In the fields of biomedicine and cell research, the separation of human blood stem cells is a key preliminary step in carrying out hematopoietic stem cell transplantation, cell therapy and basic medical research. Centrifugal separation devices have become the mainstream equipment because they can achieve efficient separation of stem cells and blood components through density gradient differences.

[0003] Traditional separation devices often use a "single tube independent fixation" structure. Operators need to place the test tubes containing blood samples into the positioning slot one by one. After separation, they need to reverse the process to unlock and remove them one by one, which forces the entire work process to be prolonged. Utility Model Content

[0004] In view of the above situation and to overcome the defects of the prior art, this utility model provides a human blood stem cell separation device, which effectively solves the problems mentioned in the background.

[0005] To achieve the above objectives, the present invention provides the following technical solution: a human blood stem cell separation device, comprising a fixed plate, a positioning seat movably installed inside the fixed plate, a connecting column fixedly connected to the bottom surface of the positioning seat, a drive bevel gear fixedly connected to the bottom end of the connecting column extending rotatably through to the bottom of the positioning seat, a plurality of test tubes movably installed inside the positioning seat, and a protective box fixedly installed on the bottom surface of the positioning seat.

[0006] Preferably, a motor is fixedly installed on one side of the inside of the protective box, and a driving bevel gear is fixedly connected to the driving end of the motor. A rotating shaft is rotatably installed on one side of the inside of the protective box, and a driven bevel gear is fixedly connected to one end of the rotating shaft. The driven bevel gear and the driving bevel gear are meshed together. A transmission bevel gear is fixedly sleeved on the middle of the outer side of the rotating shaft. The transmission bevel gear and the driving bevel gear are meshed together.

[0007] Preferably, the positioning seat is externally fixedly mounted with an array of fixed columns, each of the four fixed columns has a first spring fixedly mounted inside, one end of each of the four first springs is fixedly connected to a sliding column, one end of each of the four sliding columns is fixedly connected to a movable ball, and an annular groove is opened inside the fixed plate, with the four movable balls respectively slidably connected inside the annular groove.

[0008] Preferably, the positioning seat has an internal movable cavity, and a vertical plate is fixedly installed inside the movable cavity. Three second springs are fixedly installed at both ends of the vertical plate. One end of each of the two sets of second springs is fixedly connected to a fixed plate. The two fixed plates are movably connected to multiple test tubes respectively. An array of adjusting shafts is rotatably connected to the top inner side of the positioning seat. The bottom ends of the multiple adjusting shafts are fixedly connected to cams. The multiple cams are movably connected to multiple fixed plates respectively. The top ends of the multiple adjusting shafts are fixedly connected to locking gears.

[0009] Preferably, a groove is provided in the middle of the top surface of the positioning seat, an L-shaped block is slidably installed inside the groove, toothed plates are fixedly installed on both sides of the outer side of the L-shaped block, the two toothed plates are respectively meshed with multiple locking gears, a fixing block is fixedly installed on one side of the top surface of the positioning seat, a bolt is threadedly connected to the top of the fixing block, one end of the L-shaped block is slidably inserted into the inside of the fixing block, and one end of the bolt is threadedly connected to the inner side of the L-shaped block.

[0010] Compared with the prior art, the beneficial effects of this utility model are: This invention uses a second spring to push a fixing plate to position the test tubes, and then pushes an L-shaped block to slide in a groove. The L-shaped block is inserted into the fixing block and fixed with bolts. The L-shaped block drives the toothed plate to slide, the toothed plate drives the locking gear to rotate, and the locking gear drives the cam to rotate, further tightening and fixing the fixing plate. This facilitates the clamping and separation of multiple test tubes, and speeds up the work process compared to processing them one by one. Attached Figure Description

[0011] The accompanying drawings are provided to further understand the present invention and form part of the specification. They are used together with the embodiments of the present invention to explain the present invention and do not constitute a limitation thereof.

[0012] In the attached diagram: Figure 1 This is a schematic diagram of the overall structure of this utility model; Figure 2 This is a schematic diagram of the connection structure of the first spring of this utility model; Figure 3 This is a schematic diagram of the cross-sectional structure of the protective box of this utility model; Figure 4 This is a schematic diagram of the internal connection structure of the movable cavity of this utility model; Figure 5 This is a schematic diagram of the connection structure of the L-shaped block of this utility model; In the diagram: 1. Fixed plate; 2. Positioning seat; 3. Connecting column; 4. Drive bevel gear; 5. Test tube; 6. Protective box; 7. Motor; 8. Driving bevel gear; 9. Rotating shaft; 10. Driven bevel gear; 11. Transmission bevel gear; 12. Fixed column; 13. First spring; 14. Sliding column; 15. Movable ball; 16. Annular groove; 17. Movable cavity; 18. Vertical plate; 19. Second spring; 20. Fixed plate; 21. Adjusting shaft; 22. Locking gear; 23. Cam; 24. Slide groove; 25. L-shaped block; 26. Toothed plate; 27. Fixed block; 28. Bolt. Detailed Implementation

[0013] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present utility model without creative effort are within the protection scope of the present utility model.

[0014] Example 1, by Figures 1-5 The present invention includes a fixed plate 1, a positioning seat 2 movably installed inside the fixed plate 1, a connecting column 3 fixedly connected to the bottom surface of the positioning seat 2, a drive bevel gear 4 rotatably extending through the bottom end of the connecting column 3 and fixedly connected to the bottom of the positioning seat 2, a plurality of test tubes 5 movably installed inside the positioning seat 2, the positioning seat 2 is used to position the test tubes 5, and a protective box 6 is fixedly installed on the bottom surface of the positioning seat 2, the protective box 6 is used to protect the plurality of components.

[0015] A motor 7 is fixedly installed on one side of the inside of the protective box 6. The drive end of the motor 7 is fixedly connected to the drive bevel gear 8. The motor 7 is used to rotate the drive bevel gear 8. A rotating shaft 9 is rotatably installed on one side of the inside of the protective box 6. One end of the rotating shaft 9 is fixedly connected to the driven bevel gear 10. The driven bevel gear 10 and the drive bevel gear 8 are meshed together. A transmission bevel gear 11 is fixedly sleeved on the middle of the outer side of the rotating shaft 9. The transmission bevel gear 11 and the drive bevel gear 4 are meshed together. The drive bevel gear 4 is used to rotate the connecting column 3.

[0016] The positioning base 2 is externally fixed with arrayed fixed columns 12. Each of the four fixed columns 12 has a first spring 13 fixedly installed inside. One end of each of the four first springs 13 is fixedly connected to a sliding column 14. The first springs 13 are used to push the sliding column 14 to move. One end of each of the four sliding columns 14 is fixedly connected to a movable ball 15. The fixed plate 1 has an annular groove 16 inside. The four movable balls 15 are slidably connected inside the annular groove 16. The movement of the movable balls 15 in the annular groove 16 helps the positioning base 2 to move stably.

[0017] The positioning seat 2 has an internal movable cavity 17. A vertical plate 18 is fixedly installed inside the movable cavity 17. Three second springs 19 are fixedly installed at both ends of the vertical plate 18. A fixed plate 20 is fixedly connected to one end of each of the two sets of second springs 19. The second springs 19 are used to push the fixed plate 20 to clamp the test tubes 5. The two fixed plates 20 are movably connected to multiple test tubes 5 respectively. An array of adjusting shafts 21 is rotatably connected to the top inner side of the positioning seat 2. A cam 23 is fixedly connected to the bottom end of each of the multiple adjusting shafts 21. The multiple cams 23 are movably connected to multiple fixed plates 20 respectively. The cams 23 are used to strengthen the fixation of the fixed plates 20. A locking gear 22 is fixedly connected to the top end of each of the multiple adjusting shafts 21.

[0018] A groove 24 is provided in the middle of the top surface of the positioning seat 2. An L-shaped block 25 is slidably installed inside the groove 24. Toothed plates 26 are fixedly installed on both sides of the outer surface of the L-shaped block 25. The L-shaped block 25 slides and drives the toothed plates 26 to move. The two toothed plates 26 are respectively engaged with multiple locking gears 22. A fixing block 27 is fixedly installed on one side of the top surface of the positioning seat 2. A bolt 28 is threadedly connected to the top of the fixing block 27. One end of the L-shaped block 25 is slidably inserted into the inside of the fixing block 27. One end of the bolt 28 is threadedly connected to the inner side of the L-shaped block 25. The bolt 28 is used to fix the L-shaped block 25.

[0019] Working principle: In use, firstly, multiple test tubes 5 are placed in the positioning seat 2. Then, the second spring 19 pushes the fixing plate 20 to position the test tubes 5. Next, the L-shaped block 25 is pushed to slide in the slide groove 24. The L-shaped block 25 is inserted into the fixing block 27 and fixed with bolts 28. Thus, the L-shaped block 25 drives the toothed plate 26 to slide. The toothed plate 26 drives the locking gear 22 to rotate. The locking gear 22 then drives the cam 23 to rotate, further pushing and fixing the fixing plate 20. Subsequently, the motor 7 rotates the driving bevel gear 8. The driving bevel gear 8 drives the driven bevel gear 10 to rotate. The driven bevel gear 10 drives the transmission bevel gear 11 to rotate. The transmission bevel gear 11 then drives the drive bevel gear 4 to rotate, thereby driving the connecting column 3 to rotate the positioning seat 2, so that the test tubes 5 in the positioning seat 2 are centrifuged and separated.

Claims

1. A human hematopoietic stem cell separation device, comprising a fixing plate (1), characterized in that: The fixed plate (1) is equipped with a positioning seat (2) inside. A connecting column (3) is fixedly connected to the bottom surface of the positioning seat (2). The bottom end of the connecting column (3) extends through and is fixedly connected to a drive bevel gear (4) below the positioning seat (2). Multiple test tubes (5) are installed inside the positioning seat (2). A protective box (6) is fixedly installed on the bottom surface of the positioning seat (2).

2. The human hematopoietic stem cell separation device according to claim 1, characterized in that: A motor (7) is fixedly installed on one side of the inside of the protective box (6). The driving end of the motor (7) is fixedly connected to the driving bevel gear (8). A rotating shaft (9) is rotatably installed on one side of the inside of the protective box (6). A driven bevel gear (10) is fixedly connected to one end of the rotating shaft (9). The driven bevel gear (10) and the driving bevel gear (8) are meshed together. A transmission bevel gear (11) is fixedly sleeved on the middle of the outer side of the rotating shaft (9). The transmission bevel gear (11) and the driving bevel gear (4) are meshed together.

3. The human hematopoietic stem cell separation device according to claim 1, characterized in that: The positioning seat (2) is fixedly installed with arrayed fixed columns (12) on the outside. Each of the four fixed columns (12) is fixedly installed with a first spring (13). Each of the four first springs (13) is fixedly connected to a sliding column (14) at one end. Each of the four sliding columns (14) is fixedly connected to a movable ball (15) at one end. An annular groove (16) is opened inside the fixed plate (1). The four movable balls (15) are slidably connected inside the annular groove (16).

4. The human hematopoietic stem cell separation device according to claim 1, characterized in that: The positioning seat (2) has an internal movable cavity (17), and a vertical plate (18) is fixedly installed inside the movable cavity (17). Three second springs (19) are fixedly installed at both ends of the vertical plate (18). One end of each of the two sets of second springs (19) is fixedly connected to a fixing plate (20). The two fixing plates (20) are movably connected to multiple test tubes (5). An array of adjustment shafts (21) is rotatably connected to the inner top of the positioning seat (2). The bottom ends of the multiple adjustment shafts (21) are fixedly connected to cams (23). The multiple cams (23) are movably connected to multiple fixing plates (20). The top ends of the multiple adjustment shafts (21) are fixedly connected to locking gears (22).

5. The human hematopoietic stem cell separation device according to claim 1, characterized in that: The top surface of the positioning seat (2) has a groove (24) in the middle. An L-shaped block (25) is slidably installed inside the groove (24). Tooth plates (26) are fixedly installed on both sides of the L-shaped block (25). The two tooth plates (26) are respectively meshed with multiple locking gears (22). A fixing block (27) is fixedly installed on one side of the top surface of the positioning seat (2). A bolt (28) is threadedly connected to the top of the fixing block (27). One end of the L-shaped block (25) is slidably inserted into the inside of the fixing block (27). One end of the bolt (28) is threadedly connected to the inside of the L-shaped block (25).

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