A chromatography column fixing device for exosome purification

Abstract

The utility model relates to exosome purification chromatographic column technical field, concretely is a kind of chromatographic column fixing device for exosome purification, including base, connecting column, mounting plate, chromatographic tube, mounting ring, limiting device and clamping device, the connecting column is fixedly connected in the upper surface of base, the mounting ring is fixedly connected in one end of mounting plate, the mounting plate is installed on the surface of connecting column by limiting device, the inside mounting ring is equipped with clamping device, the chromatographic tube is installed in the inside of mounting ring by clamping device, the limiting device is installed on the surface of connecting column, the limiting device includes mounting block and adjusting groove, the mounting block is slidably connected on the surface of connecting column, the adjusting groove is opened in the surface of connecting column, the surface of connecting column is equipped with sliding slot, the inside fixed connection of mounting block has slider. The utility model limiting device, the stable installation of mounting plate is realized, and the stability of equipment mounting plate is improved.

Description

Technical Field

[0001] This utility model relates to the field of exosome purification chromatography column technology, and in particular to a chromatography column fixation device for exosome purification. Background Technology

[0002] Exosomes are nanoscale vesicles with a diameter of 30-150 nm secreted by cells and are widely present in bodily fluids such as blood, urine, and saliva. Chromatography columns for exosome purification are the core tools for the separation and purification of exosomes. Their core function is to achieve efficient separation of exosomes from impurities such as proteins and nucleic acids in bodily fluids based on their size, density, or surface antigen characteristics, thereby obtaining high-purity and highly active exosomes suitable for biomedical research, disease diagnosis, and drug carrier preparation.

[0003] However, the existing equipment has the following shortcomings: When adjusting the mounting plate and mounting ring on the surface of the connecting column, the existing equipment generally uses bolts to abut and fix the mounting plate on the surface of the connecting column. After long-term use, the threaded rod is prone to stripping, causing the mounting plate to shake on the surface of the connecting column, making it difficult to securely install the chromatography tube inside the mounting ring at one end of the mounting plate.

[0004] Therefore, we propose a chromatography column immobilization device for exosome purification to address the problems mentioned above. Utility Model Content

[0005] The purpose of this invention is to address the shortcomings of existing technologies where, during prolonged use, the threaded rod limiting the mounting plate is prone to stripping, causing the mounting plate to wobble on the connecting column surface and making it difficult to securely install the chromatography tube. Therefore, this invention proposes a chromatography column fixing device for exosome purification.

[0006] To achieve the above objectives, this utility model adopts the following technical solution: a chromatography column fixing device for exosome purification, comprising a base, a connecting column, a mounting plate, a chromatography tube, a mounting ring, a limiting device, and a clamping device. The connecting column is fixedly connected to the upper surface of the base, the mounting ring is fixedly connected to one end of the mounting plate, the mounting plate is mounted on the surface of the connecting column via the limiting device, a clamping device is installed inside the mounting ring, the chromatography tube is mounted inside the mounting ring via the clamping device, the limiting device is mounted on the surface of the connecting column, the limiting device includes a mounting block and an adjusting groove, the mounting block is slidably connected to the surface of the connecting column, the adjusting groove is formed on the surface of the connecting column, a sliding groove is formed on the surface of the connecting column, a slider is fixedly connected inside the mounting block, the slider is slidably connected inside the sliding groove, a pull hole is formed on the surface of the mounting block, an adjusting rod is slidably connected inside the pull hole, one end of the adjusting rod is inserted into the inside of the adjusting groove, and a moving block is fixedly connected to the end of the adjusting rod away from the adjusting groove.

[0007] Furthermore, a first spring is sleeved and connected to the surface of the adjusting rod. By setting the adjusting rod, the mounting block can be limited, reducing the difficulty of accurately positioning the mounting block on the surface of the connecting column during use.

[0008] Furthermore, one end of the first spring is fixedly connected to the surface of the moving block, and the end of the first spring away from the moving block is fixedly connected to the inside of the pull hole. The first spring is provided to facilitate the application of a reset force to the adjusting rod.

[0009] Furthermore, a handle is fixedly connected to the end of the adjusting rod away from the adjusting groove, and the handle is provided to facilitate the movement of the adjusting rod.

[0010] Furthermore, the clamping device is installed inside the mounting ring. The clamping device includes a contact ring and a connecting ring. The contact ring is fitted to the surface of the chromatography tube. The connecting ring is fixedly connected to the end of the contact ring away from the chromatography tube. A movable plate is fixedly connected to the end of the connecting ring away from the contact ring. The movable plate is slidably connected inside the mounting ring. A guide rod is fixedly connected to the end of the connecting ring away from the contact ring. A protrusion is fixedly connected to the outer surface of the mounting ring. The guide rod passes through the mounting ring and the protrusion. A connecting plate is fixedly connected to the end of the movable plate away from the connecting ring. Both ends of the connecting plate are fixedly connected to the end of the guide rod away from the connecting ring.

[0011] Furthermore, a second spring is sleeved and connected to the surface of the guide rod, and the guide rod is provided to provide a telescopic track for the second spring.

[0012] Furthermore, one end of the second spring is fixedly connected to the surface of the connecting plate, and the other end of the second spring away from the connecting plate is fixedly connected to the surface of the protrusion. The second spring is provided to facilitate the application of a reset force to the moving plate.

[0013] Compared with the prior art, the advantages and positive effects of this utility model are as follows:

[0014] 1. In this utility model, by setting a limiting device, the mounting plate is effectively fixed, which plays a role in quickly installing the mounting plate and the mounting ring. This reduces the need for existing equipment to use bolts for abutment installation when installing the mounting plate and the connecting column surface. Over time, the bolts are prone to stripping, causing the mounting plate to shake during use and affecting the stability of the mounting ring on the chromatography tube. This limiting device achieves stable installation of the mounting plate and improves the stability of the equipment mounting plate.

[0015] 2. In this utility model, by setting a clamping device, the chromatography tube can be quickly installed effectively, thus playing the role of clamping the chromatography tube. This reduces the problem of poor equipment compatibility caused by the fixed installation end of the chromatography tube when installing it. This clamping device enables the rapid installation of different types of chromatography tubes and improves the equipment's compatibility. Attached Figure Description

[0016] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the accompanying drawings used in the description of the embodiments or the prior art will be briefly introduced below.

[0017] Figure 1 This invention provides a three-dimensional structural schematic diagram of a chromatography column immobilization device for exosome purification;

[0018] Figure 2 This invention provides a side view of a chromatography column immobilization device for exosome purification.

[0019] Figure 3 This invention provides a partial structural schematic diagram of a chromatography column immobilization device for exosome purification;

[0020] Figure 4 This invention provides a chromatography column immobilization device for exosome purification. Figure 3 Enlarged structural diagram at point A in the middle;

[0021] Figure 5 This invention provides a chromatography column immobilization device for exosome purification. Figure 3 Enlarged structural diagram at point B.

[0022] Legend: 1. Base; 2. Connecting column; 3. Mounting plate; 4. Chromatography tube; 5. Mounting ring; 6. Limiting device; 61. Mounting block; 62. Adjusting groove; 63. Slide groove; 64. Sliding block; 65. Pull hole; 66. Adjusting rod; 67. Moving block; 68. First spring; 69. Pull handle; 7. Clamping device; 71. Contact ring; 72. Connecting ring; 73. Guide rod; 74. Moving plate; 75. Connecting plate; 76. Protrusion; 77. Second spring. Detailed Implementation

[0023] The embodiments of the present invention are described in detail below. Examples of the embodiments are shown in the accompanying drawings. The embodiments described below with reference to the accompanying drawings are exemplary and intended to explain the present invention, but should not be construed as limiting the present invention.

[0024] Please see Figures 1-5This utility model provides a technical solution: a chromatography column fixing device for exosome purification, including a base 1, a connecting column 2, a mounting plate 3, a chromatography tube 4, a mounting ring 5, a limiting device 6, and a clamping device 7. The connecting column 2 is fixedly connected to the upper surface of the base 1, the mounting ring 5 is fixedly connected to one end of the mounting plate 3, the mounting plate 3 is mounted on the surface of the connecting column 2 through the limiting device 6, the clamping device 7 is installed inside the mounting ring 5, and the chromatography tube 4 is installed inside the mounting ring 5 through the clamping device 7.

[0025] The base 1 serves as the overall support foundation for the device, made of high-density anti-slip material to ensure stable and wobbly placement. The connecting column 2 supports and lifts the mounting plate 3, made of high-strength, corrosion-resistant material with precision surface machining to ensure smooth sliding of the mounting block 61. The mounting plate 3 serves as the fixing carrier for the mounting ring 5, providing a secure connection and allowing the chromatography tube 4 to adjust its height synchronously. The chromatography tube 4 is the core component for exosome purification, filled with chromatography medium to achieve precise separation and purification of exosomes. The mounting ring 5 forms the mounting frame for the clamping device 7, with a regular structure that provides installation space for the chromatography tube 4. The limiting device 6 securely locks the mounting plate 3 onto the surface of the connecting column 2, ensuring the stability of the chromatography tube 4. The clamping device 7 can adaptively clamp different models of chromatography tubes 4, balancing stability and adaptability to meet diverse experimental needs.

[0026] The specific setup and function of its first and second devices will be described in detail below.

[0027] In this implementation scheme: the limiting device 6 is installed on the surface of the connecting column 2. The limiting device 6 includes a mounting block 61 and an adjusting groove 62. The mounting block 61 is a connecting and fixing component of the mounting plate 3, and has an internal mounting structure adapted to the mounting plate 3 to ensure that the mounting plate 3 is fixed without loosening. The adjusting groove 62 is a locking groove for the adjusting rod 66, which is evenly distributed along the length of the connecting column 2, and can realize multi-level height adjustment of the mounting plate 3. The mounting block 61 is slidably connected to the surface of the connecting column 2, and the adjusting groove 62 is opened on the surface of the connecting column 2. A slide groove 63 is provided, which serves as a guide channel for the slider 64 to ensure accurate movement of the mounting block 61. The slider 64 is fixedly connected inside the mounting block 61. The slider 64 is sized to match the slide groove 63 to prevent the mounting block 61 from rotating. The slider 64 slides within the slide groove 63. A pull hole 65 is provided on the surface of the mounting block 61. An adjusting rod 66 is slidably connected inside the pull hole 65. One end of the adjusting rod 66 is inserted into the adjusting groove 62, and the end of the adjusting rod 66 away from the adjusting groove 62 is fixedly connected to a moving block 67.

[0028] Specifically, a first spring 68 is sleeved and connected to the surface of the adjusting rod 66. The first spring 68 is a compression spring made of high-elasticity stainless steel, which has long-lasting elasticity and can provide stable return force.

[0029] In this embodiment, by setting the adjusting rod 66, the mounting block 61 can be limited, reducing the difficulty of accurately positioning the mounting block 61 on the surface of the connecting column 2 during use.

[0030] Specifically, one end of the first spring 68 is fixedly connected to the surface of the moving block 67, and the other end of the first spring 68 away from the moving block 67 is fixedly connected to the inside of the pull hole 65. The first spring 68 is provided to facilitate the application of a reset force to the adjusting rod 66.

[0031] Specifically, a handle 69 is fixedly connected to the end of the adjusting rod 66 away from the adjusting groove 62. The handle 69 is provided to facilitate the movement of the adjusting rod 66.

[0032] In this embodiment: the clamping device 7 is installed inside the mounting ring 5. The clamping device 7 includes a contact ring 71 and a connecting ring 72. The connecting ring 72 is a transition component connecting the contact ring 71 and the moving plate 74. It has high transmission efficiency and ensures uniform transmission of clamping force. The contact ring 71 is attached to the surface of the chromatography tube 4. The connecting ring 72 is fixedly connected to the end of the contact ring 71 away from the chromatography tube 4. The moving plate 74 is fixedly connected to the end of the connecting ring 72 away from the contact ring 71. The moving plate 74 is slidably connected inside the mounting ring 5. The guide rod 73 is fixedly connected to the end of the connecting ring 72 away from the contact ring 71. A protrusion 76 is fixedly connected to the outer surface of the mounting ring 5. The guide rod 73 passes through the mounting ring 5 and the protrusion 76. The connecting plate 75 is fixedly connected to the end of the moving plate 74 away from the connecting ring 72. The two ends of the connecting plate 75 are fixedly connected to the end of the guide rod 73 away from the connecting ring 72.

[0033] In this embodiment: By setting a contact ring 71, which is a direct clamping component of the chromatography tube 4, the contact ring 71 is made of elastic wear-resistant rubber material with a smooth and burr-free surface, which can avoid damaging the outer wall of the chromatography tube 4, while increasing the friction with the tube wall and improving the clamping stability.

[0034] Specifically, a second spring 77 is sleeved and connected to the surface of the guide rod 73, and the guide rod 73 is provided to provide a telescopic track for the second spring 77.

[0035] Specifically, one end of the second spring 77 is fixedly connected to the surface of the connecting plate 75, and the other end of the second spring 77 away from the connecting plate 75 is fixedly connected to the surface of the protrusion 76. The second spring 77 is provided to facilitate the application of a reset spring force to the moving plate 74.

[0036] Working principle: When using the equipment, the user installs the mounting plate 3 inside the mounting block 61. Then, the user pulls the handle 69, which moves the adjusting rod 66 to move the moving block 67. As the moving block 67 moves, it compresses the first spring 68, generating elastic force. This causes the mounting block 61 to fit onto the surface of the connecting column 2. The slider 64 is aligned with the groove 63 and moved downwards. After the mounting block 61 reaches a suitable height on the surface of the connecting column 2, the user releases the handle 69. The first spring 68 then returns to its original position, pressing against the moving block 67 and moving the adjusting rod 66 to insert it into the adjusting groove 62 on the surface of the connecting column 2, mechanically locking the mounting block 61. The user then pulls the connecting plate 75, which moves the guide rod 73 and the moving plate 74. As the connecting plate 75 moves, it stretches the second spring 77, generating elastic force, which then moves the chromatography tube 4... The plate is placed in the mounting ring 5, and then the connecting plate 75 is released. The second spring 77 returns to its original position and abuts the moving plate 74, which drives the connecting ring 72 and the contact ring 71 to move and clamp the surface of the chromatography tube 4. The exosomes are then poured into the chromatography tube 4, and the collection cup is placed at the discharge end of the lower end of the chromatography tube 4 to purify the exosomes. By setting the limiting device 6, the mounting plate 3 is effectively fixed, which plays a role in the quick installation of the mounting plate 3 and the mounting ring 5. This reduces the problem that existing equipment generally uses bolts to abut the mounting plate 3 and the connecting column 2. The bolts are prone to stripping after long-term use, causing the mounting plate 3 to shake during use and affecting the stability of the mounting ring 5 on the chromatography tube 4. This limiting device 6 achieves a stable installation of the mounting plate 3 and improves the stability of the equipment mounting plate 3.

[0037] By setting up the clamping device 7, the chromatography tube 4 can be quickly installed effectively, thus clamping the chromatography tube 4. This reduces the difficulty of clamping and installing different models of chromatography tube 4 in existing equipment due to the fixed installation end of the chromatography tube 4, resulting in poor equipment adaptability. This clamping device 7 enables the rapid installation of different models of chromatography tube 4, improving the adaptability of the equipment.

[0038] The above description discloses only one or more preferred embodiments of the present utility model and should not be construed as limiting the scope of the present utility model. Those skilled in the art can understand that implementing all or part of the above embodiments and making equivalent changes in accordance with the claims of the present utility model still fall within the scope of the present utility model.

Claims

1. A chromatography column fixing device for exosome purification, comprising a base (1), a connecting column (2), a mounting plate (3), a chromatography tube (4), a mounting ring (5), a limiting device (6), and a clamping device (7), characterized in that: The connecting column (2) is fixedly connected to the upper surface of the base (1), the mounting ring (5) is fixedly connected to one end of the mounting plate (3), the mounting plate (3) is mounted on the surface of the connecting column (2) by a limiting device (6), a clamping device (7) is installed inside the mounting ring (5), and the chromatography tube (4) is installed inside the mounting ring (5) by the clamping device (7). The limiting device (6) is installed on the surface of the connecting column (2). The limiting device (6) includes a mounting block (61) and an adjusting groove (62). The mounting block (61) is slidably connected to the surface of the connecting column (2). The adjusting groove (62) is opened on the surface of the connecting column (2). A sliding groove (63) is opened on the surface of the connecting column (2). A slider (64) is fixedly connected inside the mounting block (61). The slider (64) is slidably connected inside the sliding groove (63). A pull hole (65) is opened on the surface of the mounting block (61). An adjusting rod (66) is slidably connected inside the pull hole (65). One end of the adjusting rod (66) is inserted into the inside of the adjusting groove (62). A moving block (67) is fixedly connected to the end of the adjusting rod (66) away from the adjusting groove (62).

2. The chromatography column immobilization device for exosome purification according to claim 1, characterized in that: The surface of the adjusting rod (66) is fitted with a first spring (68).

3. The chromatography column immobilization device for exosome purification according to claim 2, characterized in that: One end of the first spring (68) is fixedly connected to the surface of the moving block (67), and the other end of the first spring (68) away from the moving block (67) is fixedly connected to the inside of the pull hole (65).

4. The chromatography column immobilization device for exosome purification according to claim 3, characterized in that: A handle (69) is fixedly connected to the end of the adjusting rod (66) away from the adjusting groove (62).

5. The chromatography column immobilization device for exosome purification according to claim 1, characterized in that: The clamping device (7) is installed inside the mounting ring (5). The clamping device (7) includes a contact ring (71) and a connecting ring (72). The contact ring (71) is attached to the surface of the chromatography tube (4). The connecting ring (72) is fixedly connected to the end of the contact ring (71) away from the chromatography tube (4). A moving plate (74) is fixedly connected to the end of the connecting ring (72) away from the contact ring (71). The moving plate (74) is slidably connected inside the mounting ring (5). A guide rod (73) is fixedly connected to the end of the connecting ring (72) away from the contact ring (71). A protrusion (76) is fixedly connected to the outer surface of the mounting ring (5). The guide rod (73) passes through the mounting ring (5) and the protrusion (76). A connecting plate (75) is fixedly connected to the end of the moving plate (74) away from the connecting ring (72). Both ends of the connecting plate (75) are fixedly connected to the end of the guide rod (73) away from the connecting ring (72).

6. The chromatography column immobilization device for exosome purification according to claim 5, characterized in that: A second spring (77) is sleeved and connected to the surface of the guide rod (73).

7. The chromatography column immobilization device for exosome purification according to claim 6, characterized in that: One end of the second spring (77) is fixedly connected to the surface of the connecting plate (75), and the other end of the second spring (77) away from the connecting plate (75) is fixedly connected to the surface of the protrusion (76).

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