An extracellular vesicle isolation device

Abstract

The utility model relates to cell separation technical field, specifically is a kind of extracellular vesicle separation device, including workbench, vibrator and dosing valve, workbench side is equipped with control panel by bolt mounting, workbench is away from the support frame side by bolt mounting support frame, the support frame side is equipped with fixed plate from top to bottom, and the fixed ring is equipped with in the fixed ring separation tank A and separation tank B respectively, separation tank A top is equipped with feed pipe by reservation mouth and bolt mounting. Control panel simultaneously starts vibrator and drives vibrator vibration operation, the resilience of the spring and telescopic link set with, can accelerate the vibration separation of sample liquid, the extracellular vesicle of sample liquid can be preliminarily separated, and dosing valve can be opened again, the flow of the sample liquid of preliminary separation is controlled, make it flow into separation tank B, carry out secondary extracellular vesicle separation, make its quality more improve.

Description

Technical Field

[0001] This utility model relates to the field of cell separation technology, specifically to an extracellular vesicle separation device. Background Technology

[0002] Extracellular vesicles are a class of membrane-bound vesicles secreted by cells, ranging from nanometer to micrometer in size. They include different types such as exosomes, microvesicles, and apoptotic bodies. The isolation and purification of extracellular vesicles is an important research area. Extracellular vesicle isolation devices and methods include ultracentrifugation, size exclusion chromatography, immunoaffinity chromatography, precipitation, microfluidics, and field flow fractionation.

[0003] Current devices are slow and incomplete in separating extracellular vesicles, and the stability of the separation device is also poor. Therefore, an extracellular vesicle separation device is proposed to facilitate multiple vibration separations of extracellular vesicles, thereby improving their quality and enhancing the stability of the separation vessel. Utility Model Content

[0004] To address the problems in the existing technology, this utility model provides an extracellular vesicle separation device that facilitates repeated vibration separation of extracellular vesicles, thereby improving their quality and enhancing the stability of the separation tank.

[0005] The technical solution adopted by this utility model to solve its technical problem is an extracellular vesicle separation device, including a workbench, a vibrator and a drug dosing valve. A control panel is installed on one side of the workbench by bolts, and a support frame is installed on the side of the workbench away from the support frame by bolts. A fixing plate is provided on one side of the support frame from top to bottom, and a fixing ring is provided at one end of the fixing plate.

[0006] Separator A and separator B are respectively provided inside the fixed ring. The top of separator A is equipped with a feed pipe through a reserved opening and bolts. A liquid outlet valve is provided between separator A and separator B. A conical discharge pipe is provided at the bottom of separator B.

[0007] Specifically, one side of the support frame is fixed with bolts, and the support plate passes through a pre-drilled opening at one end of the fixed plate. A bolt A is tightened through a pre-drilled threaded hole at the top of the fixed plate corresponding to the top of the support plate. One end of the support plate is connected to a fixing ring with bolts, and a reinforcing column is installed at the bottom of the fixed plate on one side of the support frame with bolts.

[0008] Specifically, an anti-slip ring is adhered to the inner wall of the fixing ring, and a tightening bolt B is inserted through the reserved openings on both sides of the fixing ring corresponding to the outer periphery of the anti-slip ring, with a silicone pad adhered to one end of the tightening bolt B.

[0009] Specifically, springs are provided on the inner walls of separation tank A and separation tank B respectively, and telescopic rods are provided on the inner circumference of the springs. One end of the springs and telescopic rods is bolted to the separation plate, and sealing rings are respectively adhered to the inner walls of separation tank A and separation tank B and the outer periphery of the separation plate. Vibrators are provided at both ends of the bottom of the separation plate.

[0010] Specifically, sealing rings are attached to the connection points between the bottom of the separator A and the top of the separator B and the liquid outlet valve, respectively, and a dosing valve is installed on one side of the top of the separator B through a reserved opening and bolts.

[0011] Specifically, the output terminal of the control panel is electrically connected to the vibrator, the liquid outlet valve, and the dosing valve via wires.

[0012] The beneficial effects of this utility model are:

[0013] (1) The extracellular vesicle separation device of this utility model has a control panel that simultaneously starts the vibrator to drive the vibrator to vibrate. The spring and telescopic rod have elasticity, which can accelerate the vibration separation of the sample liquid and perform preliminary separation of the extracellular vesicles in the sample liquid. Then, opening the dosing valve can control the flow rate of the initially separated sample liquid, so that it flows into the separation tank B for secondary separation of extracellular vesicles, thereby further improving its quality.

[0014] (2) The extracellular vesicle separation device of this utility model places separation tank A and separation tank B in a fixing ring, and rotates the anti-slip ring to fix and limit separation tank A and separation tank B. The anti-slip ring of the inner ring is close to the outer periphery of separation tank A and separation tank B, increasing the friction with the inner wall of the fixing ring, further improving the stability of fixing the separation tank, and facilitating disassembly. Attached Figure Description

[0015] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0016] Figure 1 This is a schematic diagram of the overall design of this utility model;

[0017] Figure 2 This is a schematic diagram of the fixing ring of this utility model;

[0018] Figure 3 This is a schematic diagram of the separation tank of this utility model;

[0019] In the diagram: 1. Workbench; 2. Control panel; 3. Support frame; 4. Fixing plate; 5. Reinforcing column; 6. Support plate; 7. Tightening bolt A; 8. Fixing ring; 9. Tightening bolt B; 10. Anti-slip ring; 11. Separator A; 12. Feed pipe; 13. Spring; 14. Telescopic rod; 15. Separator plate; 16. Vibrator; 17. Discharge valve; 18. Separator B; 19. Dosing valve. Detailed Implementation

[0020] To make the technical means, creative features, objectives and effects of this utility model easier to understand, the present utility model will be further described below in conjunction with specific embodiments.

[0021] To facilitate repeated vibrational separation of extracellular vesicles, thereby improving their quality and enhancing the stability of the separation vessel, such as... Figure 1-3 As shown, the extracellular vesicle separation device of this utility model includes a workbench 1, a vibrator 16 and a dosing valve 19. A control panel 2 is bolted to one side of the workbench 1, and a support frame 3 is bolted to the side of the workbench 1 away from the support frame 3. A fixing plate 4 is provided on one side of the support frame 3 from top to bottom, and a fixing ring 8 is provided at one end of the fixing plate 4.

[0022] Separating tanks A11 and B18 are respectively provided in the fixing ring 8. The top of separating tank A11 is equipped with a feed pipe 12 through a reserved opening and bolts. A liquid outlet valve 17 is provided between separating tank A11 and separating tank B18. A conical discharge pipe is provided at the bottom of separating tank B18.

[0023] In use, the sample liquid is added into the separation tank A11 through the feed pipe 12. The sample liquid is filtered through the separation plate 15. The vibrator 16 is started at the same time through the control panel 2 to drive the vibrator 16 to vibrate. The spring 13 and the telescopic rod 14 have elasticity, which can accelerate the vibration separation of the sample liquid and can perform preliminary separation of extracellular vesicles in the sample liquid.

[0024] Opening the dosing valve 19 can control the flow rate of the initially separated sample liquid, allowing it to flow into the separation tank B18. At the same time, opening the dosing valve 19 adds the separation drug solution into the separation tank B18 for secondary separation of extracellular vesicles, further improving its quality.

[0025] Pulling the support plate 6 adjusts the distance between it and the fixed plate 4, and rotating and tightening the bolt A7 fixes it. The distance of the fixing ring 8 can be adjusted, and the reinforcing column 5 can help improve the support force at the bottom of the fixed plate 4.

[0026] Before adding the sample solution, place separation tanks A11 and B18 inside the fixing ring 8. Rotate the anti-slip ring 10 to fix and limit separation tanks A11 and B18. The inner anti-slip ring 10 is tightly attached to the outer circumference of separation tanks A11 and B18 to increase the friction with the inner wall of the fixing ring 8 and further improve the fixing effect. Then, bolt the outlet valve 17 between separation tanks A11 and B18 to facilitate separation operations.

[0027] To facilitate the disassembly and installation of the separator, for example, such as Figure 1 , Figure 2 , Figure 3 As shown, this utility model also includes a fixed plate 4 mounted on one side of the support frame 3 by bolts. The fixed plate 4 has a pre-reserved opening at one end through which the support plate 6 passes, and the top of the fixed plate 4 has a pre-reserved threaded hole corresponding to the top of the support plate 6 through which a tightening bolt A7 passes. One end of the support plate 6 is connected to the fixing ring 8 by bolts, and a reinforcing column 5 is mounted on the bottom of the fixed plate 4 on one side of the support frame 3 by bolts.

[0028] When in use, pull the support plate 6 to adjust the distance between it and the fixed plate 4, and rotate and tighten the bolt A7 to fix it. The distance of the fixing ring 8 can be adjusted. The reinforcing column 5 can help improve the support force at the bottom of the fixed plate 4.

[0029] For example, such as Figure 1 , Figure 2 , Figure 3 As shown, this utility model also includes an anti-slip ring 10 adhered to the inner wall of the fixing ring 8, and a tightening bolt B9 passing through the reserved openings on both sides of the fixing ring 8 corresponding to the outer periphery of the anti-slip ring 10, with a silicone pad adhered to one end of the tightening bolt B9.

[0030] When in use, separator A11 and separator B18 are placed inside the fixing ring 8. The anti-slip ring 10 is rotated to fix and limit separator A11 and separator B18. The inner anti-slip ring 10 is tightly attached to the outer circumference of separator A11 and separator B18, increasing the friction with the inner wall of the fixing ring 8 and further improving the fixing effect.

[0031] For example, such as Figure 1 , Figure 2 , Figure 3 As shown, the present invention also includes springs 13 on the inner walls of the separation tanks A11 and B18, telescopic rods 14 on the inner circumference of the springs 13, and separation plates 15 mounted on one end of the springs 13 and telescopic rods 14 by bolts. Sealing rings are respectively adhered to the inner walls of the separation tanks A11 and B18 and the periphery of the separation plates 15. Vibrators 16 are provided at both ends of the bottom of the separation plates 15.

[0032] In use, the sample liquid is filtered through the separation plate 15, and the vibrator 16 is activated to vibrate. The spring 13 and the telescopic rod 14 have elasticity, which can accelerate the vibration separation of the sample liquid and perform preliminary separation of the extracellular vesicles in the sample liquid.

[0033] For example, such as Figure 1 , Figure 2 , Figure 3As shown, the present invention also includes sealing rings attached to the connection between the bottom of the separator A11 and the top of the separator B18 and the outlet valve 17, respectively, and a dosing valve 19 is installed on one side of the top of the separator B18 through a reserved opening and bolts.

[0034] During use, the flow rate of the sample liquid can be controlled by setting the dosing valve 19.

[0035] For example, such as Figure 1 , Figure 2 , Figure 3 As shown, this utility model also includes an electrical connection between the output terminal of the control panel 2 and the vibrator 16, the liquid outlet valve 17 and the dosing valve 19 via wires.

[0036] During use, the control panel 2 simultaneously activates the vibrator 16 to accelerate the separation of extracellular vesicles in the sample liquid.

[0037] In use, the sample liquid is added into the separation tank A11 through the feed pipe 12. The sample liquid is filtered through the separation plate 15. The vibrator 16 is started simultaneously through the control panel 2 to drive the vibrator 16 to vibrate. The spring 13 and the telescopic rod 14 have elasticity, which can accelerate the vibration separation of the sample liquid and perform preliminary separation of extracellular vesicles in the sample liquid.

[0038] Opening the dosing valve 19 can control the flow rate of the initially separated sample liquid, allowing it to flow into the separation tank B18. At the same time, opening the dosing valve 19 adds the separation drug solution into the separation tank B18 for secondary separation of extracellular vesicles, further improving its quality.

[0039] Pulling the support plate 6 adjusts the distance between it and the fixed plate 4, and rotating and tightening the bolt A7 fixes it. The distance of the fixing ring 8 can be adjusted, and the reinforcing column 5 can help improve the support force at the bottom of the fixed plate 4.

[0040] Before adding the sample solution, place separation tanks A11 and B18 inside the fixing ring 8. Rotate the anti-slip ring 10 to fix and limit separation tanks A11 and B18. The inner anti-slip ring 10 is tightly attached to the outer circumference of separation tanks A11 and B18 to increase the friction with the inner wall of the fixing ring 8 and further improve the fixing effect. Then, bolt the outlet valve 17 between separation tanks A11 and B18 to facilitate separation operations.

[0041] The foregoing has shown and described the basic principles, main features, and advantages of this utility model. Those skilled in the art should understand that this utility model is not limited to the above embodiments. The descriptions of the above embodiments and specifications are merely illustrative of the principles of this utility model. Various changes and modifications can be made to this utility model without departing from its spirit and scope, and all such changes and modifications fall within the scope of protection claimed by this utility model. The scope of protection of this utility model is defined by the appended claims and their equivalents.

Claims

1. An extracellular vesicle separation device, characterized in that, The device includes a workbench (1), a vibrator (16), and a dosing valve (19). A control panel (2) is bolted to one side of the workbench (1), and a support frame (3) is bolted to the side of the workbench (1) away from the support frame (3). A fixing plate (4) is provided on one side of the support frame (3) from top to bottom, and a fixing ring (8) is provided at one end of the fixing plate (4). Separating tank A (11) and separating tank B (18) are respectively provided in the fixing ring (8). The top of separating tank A (11) is fitted with a feed pipe (12) through a reserved opening and bolts. A liquid outlet valve (17) is provided between separating tank A (11) and separating tank B (18). A conical discharge pipe is provided at the bottom of separating tank B (18).

2. The extracellular vesicle separation device according to claim 1, characterized in that, The support frame (3) is fixed with a bolt on one side. The support plate (6) is inserted through a pre-drilled hole at one end of the fixed plate (4). The bolt A (7) is inserted through the pre-drilled threaded hole at the top of the fixed plate (4) corresponding to the top of the support plate (6). One end of the support plate (6) is connected to the fixing ring (8) with a bolt. The bottom of the support frame (3) corresponding to the bottom of the fixed plate (4) is installed with a reinforcing column (5) with a bolt.

3. The extracellular vesicle separation device according to claim 1, characterized in that, The inner wall of the fixing ring (8) is adhered with an anti-slip ring (10), and the two sides of the fixing ring (8) are connected to the reserved openings on the outer periphery of the anti-slip ring (10) by a tightening bolt B (9), and one end of the tightening bolt B (9) is adhered with a silicone pad.

4. The extracellular vesicle separation device according to claim 1, characterized in that, The inner walls of the separation tank A (11) and the separation tank B (18) are respectively provided with springs (13), and the inner circumference of the springs (13) is provided with telescopic rods (14). One end of the springs (13) and the telescopic rods (14) is bolted to the separation plate (15). The inner walls of the separation tanks A (11) and B (18) and the periphery of the separation plate (15) are respectively adhered with sealing rings. Both ends of the bottom of the separation plate (15) are provided with vibrators (16).

5. The extracellular vesicle separation device according to claim 1, characterized in that, Sealing rings are attached to the connection between the bottom of the separator A (11) and the top of the separator B (18) and the outlet valve (17). A dosing valve (19) is installed on the top side of the separator B (18) through a reserved opening and bolts.

6. The extracellular vesicle separation device according to claim 1, characterized in that, The output end of the control panel (2) is electrically connected to the vibrator (16), the liquid outlet valve (17), and the dosing valve (19) via wires.

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