A series type nucleic acid extraction device

Abstract

The utility model relates to biomedical detection equipment technical field, concretely is a kind of series nucleic acid extraction device, including the reaction cavity pipe of multicavity, two ends are equipped with sealing screw cap and the sealing membrane equipped thereon, hollow tube body is equipped with several groups annular buckle assembly along the axial direction, multiple reaction chambers are sequentially formed between two adjacent annular buckle assembly, different reaction medium is encapsulated in each reaction chamber, magnetic bead preservation solution is encapsulated in the first reaction chamber, and several magnetic beads carrying DNA fragments are suspended in magnetic bead preservation solution. Realize stable independent reaction medium storage and reaction environment. The reaction cavity pipe is provided with integrated magnet bar assembly which is separated from each other and can sequentially pierce annular buckle assembly along the axial direction, which is dynamically sealed with sealing screw cap and each annular buckle assembly respectively, DNA fragments on magnetic beads react with each reaction medium respectively, reaction medium is easy to store, convenient to transfer and carry, and the dependence on professional environment and personnel is low, and extraction operation can be performed in real time and on site.

Description

Technical Field

[0001] This utility model relates to the field of biomedical testing equipment technology, specifically a tandem nucleic acid extraction device. Background Technology

[0002] Nucleic acid extraction and purification techniques using fiberglass tubes hold a fundamental position in molecular biology research. Nucleic acids, as biomolecules carrying the genetic information of organisms, are one of the basic substances for life activities. Whether performing sequence and structural analysis of nucleic acids or exploring gene function and expression regulation, the first step is always the effective isolation and purification of the target nucleic acid. The quality of the nucleic acid product obtained in this step directly affects the feasibility and accuracy of subsequent experimental studies (such as polymerase chain reaction, sequencing, etc.).

[0003] Currently, various nucleic acid extraction and purification methods exist in existing technologies, involving different processing methods such as biological lysis, chemical denaturation, or mechanical disruption. Although the specific procedures vary, their basic processes generally include: lysing cells to release their contents, separating and purifying nucleic acids from other cellular components, and enriching nucleic acids through precipitation or adsorption. The realization of these steps all depends on the sequential participation of multiple specific reagents. Through a series of reactions occurring between different reagents, the separation and purification of nucleic acids are ultimately achieved.

[0004] The current technical limitations lie in the fact that the aforementioned nucleic acid extraction and purification operations typically require specialized laboratories and can only be performed by operators using dedicated nucleic acid extraction equipment. This dependence on specific locations and specialized equipment significantly restricts the application of this technology in non-laboratory environments or emergency scenarios. Furthermore, it is usually impossible to pre-package, integrate, and combine the various reagents necessary for nucleic acid extraction into a single portable device, resulting in complex procedures and a high degree of dependence on specialized environments and personnel. Summary of the Invention

[0005] The purpose of this invention is to provide a tandem nucleic acid extraction device to solve the problems mentioned in the background art. This invention uses an integrated magnetic rod assembly that is movably housed in the reaction chamber. The reaction medium is integrated into a single portable device and reacts sequentially with the reaction medium in each chamber. This has the advantages of achieving rapid nucleic acid extraction in a sealed environment and greatly reducing the risk of contamination of the extracted nucleic acid.

[0006] To achieve the above objectives, this utility model provides the following technical solution: a tandem nucleic acid extraction device, comprising a multi-chamber reaction tube, the reaction tube being a hollow tube body, one end of which is a closed end and the other end of which is an open end, both the open and closed ends being provided with sealing caps, the caps of which are provided with sealing films, the hollow tube body being provided with several sets of annular snap-fit ​​assemblies along the axial direction, and multiple reaction chambers being sequentially formed between two adjacent annular snap-fit ​​assemblies, each reaction chamber encapsulating a different reaction medium, the first reaction chamber encapsulating a magnetic bead preservation solution, and several magnetic beads carrying DNA fragments suspended in the magnetic bead preservation solution; the sealing caps are closed at the open end to achieve a stable reaction medium storage environment, each reaction chamber is isolated by the annular snap-fit ​​assemblies, each different reaction medium is in an independent storage state, and the reaction process does not interfere with each other.

[0007] The reaction chamber is equipped with an integrated magnetic rod assembly that is separable from each other and can sequentially pierce the annular snap-fit ​​components along the axial direction. The integrated magnetic rod assembly is movably housed within the reaction chamber and dynamically sealed to the sealing nut and each annular snap-fit ​​component. The integrated magnetic rod assembly enters the first reaction chamber and magnetically attracts a magnetic bead. After the bead loses its magnetism, it is released into the next reaction chamber. The outside of the reaction chamber is equipped with a magnetic attraction component that rotates circumferentially around the tube body. The integrated magnetic rod assembly sequentially passes through each reaction chamber, and the DNA fragments on the magnetic beads react with each reaction medium independently. This process achieves extraction in a sealed environment without interference, greatly reducing the risk of contamination. The reaction medium is easy to seal, transfer, and carry, with low dependence on specialized environments and personnel, enabling real-time, on-site extraction operations.

[0008] As a further embodiment of this invention, the integrated magnetic rod assembly includes a magnetic rod and a non-magnetic magnetic rod sleeve fitted over the magnetic rod. The length of the magnetic rod sleeve is greater than the length of the reaction chamber tube. An annular groove is provided on the outer surface of the magnetic rod sleeve, and several magnetic beads carrying DNA fragments are adsorbed into the annular groove. The magnetic rod is removed from the magnetic rod sleeve in each reaction chamber, and the magnetic beads, after losing magnetism, fall into the reaction medium within the reaction chamber and remain suspended. The magnetic rod and magnetic beads are respectively positioned inside and on the outer wall of the magnetic rod sleeve, but do not contact each other, avoiding damage to the cleanliness and integrity of the DNA fragments on the surface of the magnetic beads. The magnetic rod can be removed from the magnetic rod sleeve at any time, allowing the magnetic beads to lose magnetism and fall into the reaction medium for full contact and extraction reaction.

[0009] As a further embodiment of this invention, the magnetic rod includes a magnetic rod body with a sharp piercing tip at its head. The piercing tip has a needle-like or conical structure and is made of magnetic stainless steel. The piercing tip can quickly pierce the sealing film, making the operation quick and convenient.

[0010] As a further embodiment of this invention, the tail end of the magnetic rod is provided with a pushing mechanism for driving the magnetic rod to reciprocate horizontally. The pushing mechanism includes a pushing motor, the shaft end of which is coaxially connected to the tail end of the magnetic rod. The pushing motor drives the magnetic rod to reciprocate along the axial direction, and the speed and position of the movement are precisely controllable.

[0011] As a further embodiment of this invention, the annular snap-fit ​​assembly includes an intermediate sealing membrane and a sealing ring arranged sequentially from the open end to the closed end. The inner diameter of the sealing ring transitions into the outer diameter of the magnetic rod sleeve. During the sealing contact between the magnetic rod sleeve and each sealing ring, the reaction medium from the previous reaction chamber is scraped away from the outer wall of the magnetic rod sleeve, ensuring the magnetic rod sleeve remains clean as it enters the next adjacent reaction chamber. This maintains that the reaction media in each chamber do not affect each other, enabling independent reaction processes and preventing mixing and contamination of the various reaction media.

[0012] As a further embodiment of this invention, the hollow tube inner wall is provided with several sets of first mounting grooves and second mounting grooves. Each intermediate sealing membrane is thermoformed circumferentially to the first mounting groove, and the sealing ring is embedded in the second mounting groove. The sealing membrane and sealing ring are precisely and stably positioned.

[0013] As a further improvement of this invention, the sealing ring and magnetic rod sleeve are made of flexible rubber, providing good sealing performance and allowing for slight deformation under different working conditions to meet experimental requirements. The sealing film is made of 0.1mm-0.2mm thick aluminum film. Aluminum film is easily melted and thermoplasticized, and can be quickly punctured by a piercing tip.

[0014] As a further embodiment of this invention, a heating unit is fitted onto the outer wall of the reaction chamber. The heating unit includes a temperature-controllable heating sleeve, and a heating block or thin-film heater is installed inside the heating sleeve. The heating block or thin-film heater is connected to a temperature control module. This heating unit enables flexible temperature control, providing convenience for nucleic acid extraction steps requiring heating and enhancing the practicality and versatility of the device.

[0015] As a further embodiment of this invention, the magnetic attraction assembly includes a magnet, one end of which is radially arranged along the reaction chamber tube, and the other end of which is connected to an annular drive component that drives the magnet to rotate circumferentially along the reaction chamber tube. As the annular drive component drives the magnet to rotate, it affects the movement of the magnetic bead, ensuring that the magnetic bead makes full contact with the reaction medium in all directions during dynamic movement.

[0016] As a further embodiment of this invention, the reaction chamber is made of a transparent, thermally conductive polymer material. The reaction chamber, from its open end to its closed end, sequentially encapsulates a magnetic bead preservation solution, a lysis buffer, a first rinsing solution, a second rinsing solution, and an elution solution. The environmental control agent in the magnetic bead preservation solution can adjust and stabilize the system's pH value and also form a buffer to create a stable environment, reducing the aforementioned damage. The pre-binding agent allows the less active functional groups on the magnetic bead surface to pre-bind with sodium ions, indirectly increasing the number of active groups and ensuring the subsequent adsorption capacity of the magnetic beads for nucleic acids. The antibacterial agent in the magnetic bead preservation solution effectively inhibits bacterial formation, avoiding adverse effects of bacteria and their nucleic acids on the magnetic beads, ensuring the cleanliness and reliability of the magnetic beads.

[0017] Compared with the prior art, the beneficial effects of this utility model are as follows: This device includes a multi-chamber reaction tube, which is a hollow tube body. One end of the hollow tube body is a closed end, and the other end is an open end. Both the open end and the closed end are provided with sealing caps, and the caps of the sealing caps are provided with sealing films. Several sets of annular snap-fit ​​assemblies are arranged along the axial direction of the hollow tube body. Multiple reaction chambers are formed sequentially between two adjacent annular snap-fit ​​assemblies. Each reaction chamber is encapsulated with a different reaction medium. The first reaction chamber is encapsulated with magnetic bead preservation solution, and several magnetic beads carrying DNA fragments are suspended in the magnetic bead preservation solution. It has the following beneficial effects: (1) The sealing nut is closed at the open end to achieve a stable reaction medium storage environment. Each reaction chamber is isolated by the ring buckle assembly, and each different reaction medium is in an independent storage state, and the reaction process does not interfere with each other.

[0018] The reaction chamber is equipped with an integrated magnetic rod assembly that consists of mutually separable components that can sequentially pierce the annular snap-fit ​​assembly along the axial direction. The integrated magnetic rod assembly is movably housed within the reaction chamber and dynamically sealed to the sealing nut and each annular snap-fit ​​assembly. Upon entering the first reaction chamber, the integrated magnetic rod assembly magnetically attracts a magnetic bead. After the bead demagnetizes, it is released into the next reaction chamber. The outside of the reaction chamber is equipped with a magnetic attraction assembly that rotates circumferentially around the chamber. The integrated magnetic rod assembly sequentially passes through each reaction chamber, allowing the DNA fragments on the magnetic beads to react with each reaction medium independently. This process is achieved in a sealed environment without interference, significantly reducing the risk of contamination. The reaction medium is easily sealed, convenient for transfer and transport, and has low dependence on specialized environments and personnel, enabling real-time, on-site extraction operations. As the annular drive component rotates the magnet, it influences the movement of the magnetic beads, ensuring comprehensive and thorough contact between the beads and the reaction medium during dynamic movement.

[0019] (3) The magnetic rod and magnetic beads of the integrated magnetic rod assembly are respectively set on the inside and outside of the magnetic rod sleeve, but do not contact each other, so as to avoid damaging the cleanliness and integrity of the DNA fragments on the surface of the magnetic beads. The magnetic rod can be removed from the magnetic rod sleeve at any time, so that the magnetic beads lose magnetism and fall into the reaction medium, and carry out the extraction reaction after full contact.

[0020] (4) The magnetic rod includes a magnetic rod body, and the head of the magnetic rod body is provided with a sharp piercing tip, which can quickly pierce the sealing film, making the operation quick and convenient.

[0021] (5) The tail of the magnetic rod is provided with a pushing mechanism that drives the magnetic rod to reciprocate horizontally. The pushing mechanism includes a pushing motor, which drives the magnetic rod to reciprocate along the axial direction. The speed and movement position are precisely controllable.

[0022] (6) During the sealing contact between the magnetic rod sleeve and each sealing ring, the reaction medium in the previous reaction chamber is scraped off the outer wall of the magnetic rod sleeve, and the magnetic rod sleeve remains clean as it enters the next adjacent reaction chamber. This ensures that the reaction media in each chamber do not affect each other, achieving independent reaction processes, and preventing mixing and contamination of the various reaction media.

[0023] (7) A heating unit is fitted on the outer wall of the reaction chamber tube. The heating unit enables flexible temperature control, which facilitates the nucleic acid extraction step that requires heating and enhances the practicality and multifunctionality of the device. Attached Figure Description

[0024] Figure 1 This is a schematic diagram of the initial assembly state of this utility model; Figure 2 This is a sectional view of the present invention along line AA; Figure 3 This is an enlarged schematic diagram of the I-node of this utility model; Figure 4 This is a schematic diagram of the closed end structure of the reaction chamber tube of this utility model; Figure 5 This is a schematic diagram of the integrated magnetic rod assembly structure of this utility model; Figure 6 A schematic diagram of the structure of the reaction chamber tube of this utility model with a heating unit sleeved on the outer wall; Figure 7 This is a schematic diagram of the structure of the reaction chamber tube of this utility model with a magnetic attraction component on the outer wall.

[0025] In the diagram: 1-Integrated magnetic rod assembly, 101-Magnetic rod, 102-Magnetic rod sleeve, 103-Piercing tip, 104-Annular groove, 2-Sealing nut, 201-Sealing membrane, 3-Reaction chamber tube, 301-Open end, 302-Closed end, 4-Heating unit, 401-Heating sleeve, 402-Thin film heater, 403-Temperature control module, 5-Magnetic suction assembly, 6-Reaction medium, 601-Magnetic bead preservation solution, 602-Cycle solution, 603-First rinsing solution, 604-Second rinsing solution, 605-Eluent, 7-Sealing ring, 8-Intermediate sealing membrane, 9-Magnetic bead, 10-Push motor shaft. Detailed Implementation

[0026] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present utility model. Example

[0027] Please see the appendix Figure 1 - Appendix Figure 5 A series-connected nucleic acid extraction device includes a multi-chamber reaction tube 3, which is made of a transparent, thermally conductive polymer material. The reaction tube is a hollow tube body, with one end designated as a closed end 302 and the other end as an open end 301. Both the open and closed ends are provided with sealing caps 2, and the caps of the sealing caps are provided with sealing membranes 201. The hollow tube body is provided with several sets of annular snap-fit ​​assemblies along the axial direction. The annular snap-fit ​​assemblies include intermediate sealing membranes 8 and sealing rings 7 arranged sequentially from the open end to the closed end. The inner diameter of the sealing rings transitions to the outer diameter of the magnetic rod sleeve. The inner wall of the hollow tube body is provided with several sets of first mounting grooves and second mounting grooves. Each intermediate sealing membrane 8 is thermoformed circumferentially to the first mounting groove, and the sealing ring 7 is embedded in the second mounting groove.

[0028] The sealing ring 7 and the magnetic rod sleeve 102 are made of flexible rubber, providing good sealing performance and allowing for slight deformation under different working conditions to meet experimental requirements. The sealing film is made of 0.2mm thick aluminum film. Aluminum film is easily melted and thermoplasticized, and can be quickly punctured by a puncture tip.

[0029] Multiple reaction chambers 1 are sequentially formed between two adjacent annular snap-fit ​​assemblies. Each reaction chamber encapsulates a different reaction medium 6. During the sealing contact between the magnetic rod sleeve 102 and each sealing ring, the reaction medium in the previous reaction chamber is scraped off the outer wall of the magnetic rod sleeve 102. The magnetic rod sleeve remains clean as it enters the next adjacent reaction chamber 1. This ensures that the reaction media in each chamber do not affect each other, achieving independent reaction processes and preventing mixing and contamination of the various reaction media.

[0030] The first reaction chamber is encapsulated with magnetic bead preservation solution 601, and several magnetic beads 9 carrying DNA fragments are suspended in the magnetic bead preservation solution; the sealing cap 2 is closed at the opening end to achieve a stable reaction medium storage environment. Each reaction chamber is isolated by a ring snap assembly, and each different reaction medium is in an independent storage state, and the reaction process does not interfere with each other.

[0031] The reaction chamber is equipped with an integrated magnetic rod assembly 1, which consists of mutually separable components that can sequentially pierce annular snap-fit ​​assemblies along the axial direction. The integrated magnetic rod assembly is movably housed within the reaction chamber and dynamically seals itself with a sealing nut and each annular snap-fit ​​assembly. Upon entering the first reaction chamber, the integrated magnetic rod assembly magnetically attracts a magnetic bead. The integrated magnetic rod assembly 1 includes a magnetic rod 101 and a non-magnetic magnetic rod sleeve 102 fitted onto the magnetic rod. The magnetic rod includes a magnetic rod body with a sharp piercing tip 103 at its head. The piercing tip is either needle-shaped or conical and is made of magnetic stainless steel. When the piercing tip touches the sealing membrane, it quickly pierces the membrane, allowing the magnetic rod and magnetic rod sleeve to enter the reaction chamber as a single unit.

[0032] The magnetic rod is equipped with a pushing mechanism at its tail end, which drives the magnetic rod to reciprocate horizontally. The pushing mechanism includes a pushing motor, and the end of the pushing motor shaft 10 is coaxially connected to the tail end of the magnetic rod. The pushing motor drives the magnetic rod to reciprocate along the axial direction, and the speed and position of the movement are precisely controllable.

[0033] The length of the magnetic rod sleeve is greater than the length of the reaction chamber tube. An annular groove 104 is provided on the outer surface of the magnetic rod sleeve. Several magnetic beads 9 carrying DNA fragments are adsorbed into the annular groove. The magnetic rod is removed from the magnetic rod sleeve 102 in each reaction chamber, and the magnetic beads, after losing magnetism, fall into the reaction medium within the reaction chamber and remain suspended. The magnetic rod 101 and magnetic beads 9 are respectively positioned inside and on the outer wall of the magnetic rod sleeve 102, but they do not contact each other to avoid damaging the cleanliness and integrity of the DNA fragments on the surface of the magnetic beads. The magnetic rod 101 can be removed from the magnetic rod sleeve 102 at any time, allowing the magnetic beads to lose magnetism and fall into the reaction medium for full contact and extraction reaction.

[0034] The reaction chamber 3 is made of a transparent, thermally conductive polymer material. The reaction chamber is encapsulated sequentially from the open end to the closed end with magnetic bead preservation solution 601, lysis solution 602, first rinsing solution 603, second rinsing solution 604, and elution solution 605.

[0035] Initially, the push motor is activated, and the motor shaft 10 propels the magnetic rod and magnetic rod sleeve to move as a unit. After the piercing tip 103 contacts the sealing membrane on the sealing nut, it quickly pierces the sealing membrane 201. The magnetic rod 101 and magnetic rod sleeve 102 first enter the first reaction chamber, which contains a magnetic bead preservation solution. The magnetic beads 9 are suspended in the magnetic bead preservation solution and are magnetically attracted to the annular groove of the magnetic rod sleeve. As the magnetic rod 101 continues to move, the piercing tip pierces the next sealing membrane and enters the next reaction chamber, which is a pyrolysis chamber. The pyrolysis solution contained in this chamber is guanidine thiocyanate. The push motor reverses, causing the magnetic rod to be pulled away from the magnetic rod sleeve. After the magnetic bead loses its magnetism, it is released into the lysis buffer 602. The DNA fragments carried on the surface of the magnetic bead react fully with guanidine thiocyanate, and the reaction is completed after a period of time.

[0036] The push motor rotates forward, driving the magnetic rod to re-enter the magnetic rod sleeve 102, and the magnetic beads are re-attached in the annular groove 104 of the magnetic rod sleeve. It continues to move forward and enters the rinsing chamber. The first rinsing solution in the rinsing chamber is guanidine thiocyanate. The push motor reverses again, causing the magnetic rod to be pulled away from the magnetic rod sleeve 102. After the magnetic bead loses its magnetism, it is released into the first rinsing solution. The DNA fragments carried on the surface of the magnetic bead react fully with the first rinsing solution 603, and the reaction is completed after a period of time.

[0037] After entering the next rinsing chamber in the above manner, the DNA fragments carried on the surface of the magnetic beads react with the first rinsing solution again, and the rinsing is completed.

[0038] The push motor reverses again, causing the magnetic rod to be pulled away from the magnetic rod sleeve. After the magnetic bead loses its magnetism, it is released into the second rinsing solution 604, which is sodium chloride. The DNA fragments carried on the surface of the magnetic bead react fully with the second rinsing solution, and the reaction is completed after a period of time.

[0039] The magnetic beads are then re-entered into the next rinsing chamber using the above method. The DNA fragments carried on the surface of the magnetic beads react again with the second rinsing solution, and the nucleic acid extraction is completed.

[0040] The push motor rotates forward, driving the magnetic rod into the magnetic rod sleeve, where it reattaches the magnetic beads to the annular groove of the sleeve. The rod continues forward into the elution chamber. The nucleic acids carried on the surface of the magnetic beads are in the elution buffer, which is typically a low-salt, neutral pH buffer (such as TE buffer or deionized water). This buffer breaks down the adsorption forces between the magnetic beads and the nucleic acids. The nucleic acids detach from the surface of the magnetic beads and dissolve in the elution buffer, thus releasing the nucleic acids.

[0041] Nucleic acid is released into the elution buffer. Finally, the push motor is started to rotate forward, driving the magnetic rod into the magnetic rod sleeve. The magnetic beads are then adsorbed into the annular groove of the magnetic rod sleeve. The sealing nut at the closed end is unscrewed, and the elution buffer carrying the nucleic acid is transferred for storage. The magnetic rod drives the magnetic rod sleeve to be pulled out of the reaction chamber again, completing the reaction. Example

[0042] Please see the appendix Figure 7 The reaction chamber tube is equipped with a magnetic attraction assembly 5 that rotates around the circumference of the tube. Each magnetic attraction assembly 5 is located at a corresponding reaction chamber. The integrated magnetic rod assembly passes through each reaction chamber in sequence.

[0043] The magnetic attraction component 5 includes a magnet, one end of which is arranged radially along the reaction chamber tube, and the other end of which is connected to an annular drive component that drives the magnet to rotate circumferentially along the reaction chamber tube.

[0044] When the magnetic bead moves within different reaction chambers, it moves the magnetic attraction assembly to the outside of the corresponding reaction chamber, activates the ring drive, and drives the magnet to rotate circumferentially.

[0045] As the annular drive component rotates the magnet, it affects the movement of the magnetic bead, allowing the magnetic bead to fully contact the reaction medium in all directions during its dynamic movement. Example

[0046] Please see the appendix Figure 6 A heating unit 4 is fitted onto the outer wall of the reaction chamber tube. The heating unit includes a temperature-controllable heating sleeve 402 and a heating block or thin-film heater 402 inside the heating sleeve. The heating block or thin-film heater is connected to a temperature control module 403. The heating block or thin-film heater is connected to a power source via wires, and its temperature is controlled by the temperature control module.

[0047] Heating unit 4 is located outside the lysis chamber and elution chamber, which activates the nucleic acid extraction reaction and enables rapid and stable release of nucleic acid.

[0048] The foregoing has shown and described the basic principles, main features, and advantages of this utility model. It will be apparent to those skilled in the art that this utility model is not limited to the details of the exemplary embodiments described above, and that it can be implemented in other specific forms without departing from the spirit or basic characteristics of this utility model. Therefore, the embodiments should be considered exemplary and non-limiting in all respects. The scope of this utility model is defined by the appended claims rather than the foregoing description, and thus all variations falling within the meaning and scope of equivalents of the claims are intended to be included within this utility model. No reference numerals in the claims should be construed as limiting the scope of the claims.

[0049] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A tandem nucleic acid extraction device, characterized in that: The reaction chamber includes a multi-chambered reaction tube (3), which is a hollow tube. One end of the hollow tube is a closed end (302), and the other end is an open end (301). Both the open end and the closed end are provided with sealing caps (2). The cap of the sealing cap is provided with a sealing film (201). The hollow tube is provided with several sets of annular snap-fit ​​components along the axial direction. Multiple reaction chambers are formed sequentially between two adjacent annular snap-fit ​​components. Each reaction chamber is encapsulated with a different reaction medium (6). The first reaction chamber is encapsulated with magnetic bead preservation solution (601). Several magnetic beads (9) carrying DNA fragments are suspended in the magnetic bead preservation solution. The reaction chamber tube (3) is provided with an integrated magnetic rod assembly (1) that is separated from each other and can be sequentially pierced by the annular snap fastener assembly along the axial direction. The integrated magnetic rod assembly is movably housed in the reaction chamber tube. The integrated magnetic rod assembly is dynamically sealed with the sealing nut and each annular snap fastener assembly. The integrated magnetic rod assembly enters the first reaction chamber and magnetically attracts the magnetic bead (9). After the magnetic bead loses its magnetism, it is released into the next reaction chamber. The outside of the reaction chamber tube is provided with a magnetic attraction assembly (5) that rotates around the tube body.

2. The tandem nucleic acid extraction device according to claim 1, characterized in that: The integrated magnetic rod assembly (1) includes a magnetic rod and a non-magnetic magnetic rod sleeve (102) sleeved on the magnetic rod (101). The length of the magnetic rod sleeve is greater than the length of the reaction chamber tube. The outer surface of the magnetic rod sleeve is provided with an annular groove (104). Several magnetic beads carrying DNA fragments are adsorbed in the annular groove. The magnetic rod is removed from the magnetic rod sleeve (102) in each reaction chamber. After the magnetic beads (9) lose magnetism, they fall into the reaction medium in the reaction chamber and are suspended.

3. The tandem nucleic acid extraction device according to claim 1, characterized in that: The magnetic rod (101) includes a magnetic rod body, the head of which is provided with a sharp piercing tip (103). The piercing tip is a needle-shaped structure or a cone-shaped structure and is made of magnetic stainless steel.

4. The tandem nucleic acid extraction device according to claim 3, characterized in that: The tail of the magnetic rod is provided with a pushing mechanism that drives the magnetic rod to reciprocate horizontally. The pushing mechanism includes a pushing motor, and the end of the pushing motor shaft (10) is coaxially connected to the tail end of the magnetic rod.

5. The tandem nucleic acid extraction device according to claim 4, characterized in that: The annular buckle assembly includes an intermediate sealing membrane (8) and a sealing ring (7) arranged sequentially from the open end to the closed end, with the inner diameter of the sealing ring transitionally fitting with the outer diameter of the magnetic rod sleeve.

6. The tandem nucleic acid extraction device according to claim 5, characterized in that: The hollow tube body is provided with several sets of first mounting grooves and second mounting grooves. Each intermediate sealing film is thermoformed around the first mounting groove in a circumferential direction, and the sealing ring is embedded in the second mounting groove.

7. The tandem nucleic acid extraction device according to claim 6, characterized in that: The sealing ring (7) and the magnetic rod sleeve (102) are made of flexible rubber, and the sealing film (201) is made of aluminum film with a thickness of 0.1mm-0.2mm.

8. The tandem nucleic acid extraction device according to claim 7, characterized in that: A heating unit (4) is fitted on the outer wall of the reaction chamber tube. The heating unit includes a temperature-controllable heating sleeve (401), a heating block or thin film heater (402) inside the heating sleeve, and a temperature control module (403) connected to the heating block or thin film heater.

9. The tandem nucleic acid extraction device according to claim 8, characterized in that: The magnetic attraction component (5) includes a magnet, one end of which is arranged radially along the reaction chamber tube, and the other end of which is connected to an annular drive component that drives the magnet to rotate circumferentially along the reaction chamber tube.

10. The tandem nucleic acid extraction device according to claim 9, characterized in that: The reaction chamber (3) is made of a transparent thermally conductive polymer material. The reaction chamber is encapsulated sequentially from the open end to the closed end with magnetic bead preservation solution (601), lysis solution (602), first rinsing solution (603), second rinsing solution (604), and elution solution (605).

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