A dosing machine

Abstract

The utility model discloses a kind of inoculation machines, belong to inoculation equipment field, including inoculation gun, one end of inoculation gun is provided with electromagnetic valve, the other end is provided with inoculation head;The other end of electromagnetic valve is arranged in hand-held part and is connected with hose, the other end of hose is connected with bacterial storage device;Inoculation gun outer wall is provided with time relay electrically connected with electromagnetic valve, button is provided on hand-held part;Inoculation head is opened on the outer wall of one side close to export end side and is provided with sliding slot, several positioning slots are equidistantly opened in the axial direction of inoculation head along sliding slot;Clamping groove is opened in positioning slot;Positioning piece is slidably arranged on inoculation head, positioning piece one side position is provided with the positioning block matched with positioning slot, locking mechanism matched with clamping groove is provided on positioning block.The utility model realizes accurate depth control by adjustable positioning piece, ensure that inoculation head stays in the upper middle part of bacterial rod, provide best germination environment for bacterial species.

Description

Technical Field

[0001] This utility model relates to the field of inoculation equipment, and in particular to a quantitative inoculation machine. Background Technology

[0002] A liquid spawn quantitative inoculation device is an automated or semi-automated alternative to traditional manual inoculation. It uses a precise pumping system and aseptic dispensing technology to inject a predetermined volume of liquid spawn (usually a mycelial ball suspension) into sterilized culture media (such as bags, bottles, or fermenters). For bagged inoculation (such as germination fungi, Armillaria mellea, and shiitake mushroom logs), the inoculation head should be inserted to a depth of 2-3 cm. This depth requires the outlet of the inoculation head to be located in the upper-middle part of the culture media after insertion into the pre-drilled hole in the bag. This is to avoid the following problems caused by inoculation that is too shallow or too deep:

[0003] 1) Easily contaminated: The bacterial solution settles near the opening on the substrate surface. This area is most susceptible to air exposure during inoculation, and the risk of contaminating bacterial spores falling in is the highest.

[0004] 2) Prone to water loss: Moisture on the substrate surface evaporates easily, resulting in a dry environment around the inoculum, which is not conducive to mycelial germination;

[0005] 3) Low utilization rate: The inoculum is concentrated on the surface and cannot fully utilize the nutrients inside the substrate bag, resulting in uneven mycelium growth;

[0006] 4) Insufficient oxygen: The bottom of the substrate has poor air permeability and low oxygen content, while mycelial growth requires sufficient oxygen in the early stage. Deep inoculation will lead to slow germination and growth.

[0007] 5) Condensation problem: After sterilization, condensation often accumulates at the bottom of the material bag. If the inoculum is injected into the water, it will be difficult to germinate due to suffocation, or even die from autolysis.

[0008] 6) Slow growth: It starts growing from the bottom and takes longer for the mycelium to cover the entire substrate bag.

[0009] Therefore, the upper middle part of the culture medium, with good air permeability, suitable temperature and humidity, away from condensation, and effectively isolated from external contaminants, is the optimal inoculation location. However, the insertion depth of traditional inoculation heads relies on manual control, which can lead to variations in insertion depth each time due to slight hand tremors, changes in posture, and fluctuations in attention. This can have multiple negative impacts on inoculation efficiency, strain survival rate, and subsequent culture results. Utility Model Content

[0010] The purpose of this invention is to provide a quantitative inoculation machine to solve the problems mentioned above.

[0011] To solve the above-mentioned technical problems, the present invention adopts the following technical solution:

[0012] This utility model discloses a quantitative inoculation machine, including an inoculation gun. One end of the inoculation gun is provided with a solenoid valve, and the other end is provided with an inoculation head. The other end of the solenoid valve is provided with a handle and connected to a flexible tube. The other end of the flexible tube is connected to a bacterial storage device. A time relay electrically connected to the solenoid valve is provided on the outer wall of the inoculation gun, and a button is provided on the handle.

[0013] A sliding groove is provided on the outer side wall of the inoculation head near the outlet end. Several positioning grooves are provided at equal intervals along the axial direction of the inoculation head. The positioning grooves are distributed perpendicularly to the sliding groove. A locking groove is provided in the positioning groove. A positioning piece is slidably provided on the inoculation head. A positioning block matching the positioning groove is provided on one side of the positioning piece. A locking mechanism that cooperates with the locking groove is provided on the positioning block.

[0014] Furthermore, the inoculation head is provided with graduation lines.

[0015] Furthermore, the locking mechanism includes an outer protective cylinder disposed inside the positioning block, a telescopic rod slidably disposed inside the outer protective cylinder, a plurality of symmetrically distributed spring limiting blocks disposed at the upper position of the bottom of the telescopic rod, and a spring sleeved on the telescopic rod located between the bottom of the outer protective cylinder and the spring limiting blocks; a lifting head fixedly connected to the telescopic rod is disposed above the outer protective cylinder.

[0016] Furthermore, the outer wall of the outer casing is provided with an external thread, and the positioning block is provided with an internal thread that mates with the external thread.

[0017] Furthermore, the number of spring limiting blocks is at least two.

[0018] Furthermore, the vertical distance between the spring limiting block and the bottom of the telescopic rod is greater than the depth of the slot.

[0019] Furthermore, the outlet end of the inoculation head is provided with a conical mounting support frame, and the bottom of the mounting support frame is provided with an upwardly convex arc-shaped protrusion.

[0020] Furthermore, a cable connecting the solenoid valve and the time relay is provided inside the grip.

[0021] Compared with the prior art, the beneficial technical effects of this utility model are as follows:

[0022] This novel quantitative inoculation machine utilizes an adjustable positioning plate. As the inoculation head descends, the mycelium log encounters this plate and is physically blocked, preventing it from continuing its descent. Through precise depth control, the inoculation head is ensured to remain in the upper-middle part of the mycelium log, providing an optimal germination environment for the spawn. Simultaneously, the positioning plate design eliminates any deviation during the inoculation head's movement, ensuring it moves strictly along a vertical straight line and avoiding variations in effective depth caused by misalignment. Furthermore, the arc-shaped protrusion design forces the spawn to disperse and splash outwards upon impact, creating a scattering effect that evenly and disperses the spawn across a three-dimensional cross-section of the culture medium. Attached Figure Description

[0023] The present invention will be further described below with reference to the accompanying drawings.

[0024] Figure 1 This is a front view of the quantitative inoculation machine of this utility model;

[0025] Figure 2 This is a schematic diagram of a partial structure of the inoculation head;

[0026] Figure 3 This is a schematic diagram of the locking mechanism.

[0027] Figure 4 This is a schematic diagram of an arc-shaped protrusion structure.

[0028] Explanation of reference numerals in the attached drawings: 1. Inoculation gun; 2. Hand grip; 3. Button; 4. Solenoid valve; 5. Time relay; 6. Hose; 7. Cable; 8. Inoculation head; 9. Positioning plate; 10. Scale line; 11. Slide groove; 12. Positioning groove; 13. Slot; 14. Positioning block; 15. Locking mechanism; 16. Mounting support frame; 17. Arc-shaped protrusion;

[0029] 1501. Outer protective sleeve; 1502. Telescopic rod; 1503. Spring limit block; 1504. Spring; 1505. Lifting head. Detailed Implementation

[0030] like Figure 1-4 As shown, a quantitative inoculation machine includes an inoculation gun 1. One end of the inoculation gun 1 is equipped with a solenoid valve 4, and the other end is equipped with an inoculation head 8. The other end of the solenoid valve 4 is mounted on a handle 2 and connected to a flexible tube 6. The other end of the flexible tube 6 is connected to a bacterial storage device. A time relay 5, electrically connected to the solenoid valve 4, is installed on the outer wall of the inoculation gun 1 to control the opening time of the solenoid valve 4. A button 3 is installed on the handle 2 for manually opening and closing the solenoid valve 4.

[0031] A groove 11 is provided on the outer side wall of the inoculation head 8 near the outlet end. A plurality of positioning grooves 12 are provided at equal intervals along the axial direction of the inoculation head 8. The positioning grooves 12 are distributed perpendicularly to the groove 11 and are arranged in the same row. A retaining groove 13 is provided in the positioning groove 12 and extends downward.

[0032] A positioning plate 9 is slidably mounted on the inoculation head 8, and the outer diameter of the positioning plate 9 is larger than the insertion port diameter on the mycelium stick. A positioning block 14 matching the positioning groove 12 is installed on one side of the positioning plate 9. The positioning block 14 moves on the sliding groove 11 and, after reaching the position of the positioning groove 12, rotates to enter the positioning groove 12, preventing the positioning plate 9 from sliding on the inoculation head 8, thus achieving the positioning function. A locking mechanism 15 that cooperates with the slot 13 is installed on the positioning block 14. The locking mechanism 15 connects the positioning block 14 and the slot 13 together, preventing the positioning block 14 from unscrewing out of the positioning groove 12 during use.

[0033] The inoculation head 8 is equipped with scale lines 10, which makes it easy to check the specific values ​​when adjusting the insertion depth.

[0034] The locking mechanism 15 includes an outer protective sleeve 1501 installed inside the positioning block 14. The outer wall of the outer protective sleeve 1501 has external threads, and the positioning block 14 has internal threads that mate with the external threads. Installation and disassembly of both are achieved by rotation. A telescopic rod 1502 is slidably installed inside the outer protective sleeve 1501. Several symmetrically distributed spring limiting blocks 1503 are installed on the upper part of the bottom of the telescopic rod 1502. The vertical distance between the spring limiting blocks 1503 and the bottom of the telescopic rod 1502 is greater than the depth of the slot 13, ensuring that the telescopic rod 1502 can be inserted into the slot 13 for locking. A spring 1504 is sleeved on the telescopic rod 1502 located between the bottom of the outer protective sleeve 1501 and the spring limiting blocks 1503. The spring 1504 allows the bottom of the telescopic rod 1502 to be inserted into the slot 13. The outer protective sleeve 1501 is equipped with a lifting head 1505 that is fixedly connected to the telescopic rod 1502. By lifting the lifting head 1505, the telescopic rod 1502 temporarily counteracts the elastic force of the spring 1504 under the action of tension and is pulled out of the slot 13, thereby engaging the locked state.

[0035] The number of spring limiting blocks 1503 is at least two.

[0036] The outlet end of the inoculation head 8 is equipped with a conical mounting support frame 16, which consists of several inclined supports. One end of the supports is welded to the inoculation head 8, and the other ends converge at a single point and are welded together. There are gaps between adjacent supports to allow liquid bacteria to be sprayed out. The bottom of the mounting support frame 16 is equipped with an upwardly convex arc-shaped protrusion 17, which is welded to the convergence point of the supports. The arc-shaped protrusion 17 has an upwardly convex arc structure on its outside, so that when the bacterial liquid is sprayed out, it hits the arc-shaped protrusion 17 and is forced to spread and splash in all directions, forming a scattering, thereby evenly and dispersedly sowing the inoculum on a three-dimensional cross-section of the culture medium.

[0037] The grip part 2 is equipped with a cable 7 that connects the solenoid valve 4 and the time relay 5.

[0038] The working process of this utility model is as follows:

[0039] First, after sterilization, the inoculation gun 1 is connected to the storage device via a flexible tube 6. Since the storage device is a pressure vessel, it has internal pressure. Next, the position of the positioning plate 9 on the inoculation head 8 is adjusted as needed, and locked in place by the positioning groove, locking slot, positioning block 14, and locking mechanism 15. Then, the inoculation head 8 is inserted into the insertion hole of the mushroom stick to be inoculated until the positioning plate 9 abuts against the outer wall of the mushroom stick and cannot move further downwards. At this point, the inoculation depth and the vertical inoculation position of the inoculation head 8 are determined. Finally, button 3 is pressed to open the solenoid valve 4. The bacterial solution is forced out by the internal pressure of the storage device and enters the inoculation gun 1 through the flexible tube 6. After passing through the inoculation head 8, it impacts the arc-shaped protrusion 17, sowing the solution in a scattering pattern on a three-dimensional cross-section of the culture medium. Simultaneously, the time relay 5 controls the solenoid valve 4 to close after a certain period, completing one inoculation. The time relay 6 controls the outflow of bacterial solution by controlling the opening time of the solenoid valve 4, ensuring a consistent outflow rate for each inoculation, achieving quantitative and uniform inoculation.

[0040] The embodiments described above are merely preferred embodiments of the present utility model and are not intended to limit the scope of the present utility model. Various modifications and improvements made to the technical solutions of the present utility model by those skilled in the art without departing from the spirit of the present utility model should fall within the protection scope defined by the claims of the present utility model.

Claims

1. A quantitative inoculation machine, characterized in that: The inoculation gun (1) is equipped with a solenoid valve (4) at one end and an inoculation head (8) at the other end; the other end of the solenoid valve (4) is located on the handle (2) and connected to a flexible tube (6), and the other end of the flexible tube (6) is connected to a storage device; a time relay (5) electrically connected to the solenoid valve (4) is provided on the outer wall of the inoculation gun (1), and a button (3) is provided on the handle (2); A sliding groove (11) is provided on the outer side wall near the outlet end of the inoculation head (8). A plurality of positioning grooves (12) are provided at equal intervals along the axial direction of the inoculation head (8). The positioning grooves (12) are perpendicular to the sliding grooves (11). A slot (13) is provided in the positioning groove (12). A positioning piece (9) is slidably provided on the inoculation head (8). A positioning block (14) matching the positioning groove (12) is provided on one side of the positioning piece (9). A locking mechanism (15) cooperating with the slot (13) is provided on the positioning block (14). The outer diameter of the positioning piece (9) is larger than the insertion port diameter on the mushroom stick. The positioning block (14) moves on the sliding groove (11) and, after reaching the position of the positioning groove (12), rotates into the positioning groove (12). The locking mechanism (15) includes an outer protective cylinder (1501) disposed inside the positioning block (14), a telescopic rod (1502) is slidably disposed inside the outer protective cylinder (1501), a plurality of symmetrically distributed spring limiting blocks (1503) are disposed at the upper position of the bottom of the telescopic rod (1502), a spring (1504) is sleeved on the telescopic rod (1502) located between the bottom of the outer protective cylinder (1501) and the spring limiting blocks (1503), and a lifting head (1505) is disposed above the outer protective cylinder (1501) and fixedly connected to the telescopic rod (1502), and the bottom of the telescopic rod (1502) is inserted into the slot (13) by the spring (1504); The outlet end of the inoculation head (8) is provided with a conical mounting support frame (16). The mounting support frame (16) is composed of several inclined supports. One end of the support is set on the inoculation head (8), and the other end converges together. There is a gap between adjacent supports for the liquid bacteria to be sprayed out. The bottom of the mounting support frame (16) is provided with an upward-protruding arc-shaped protrusion (17), so that the bacterial liquid hits the arc-shaped protrusion (17) after it is flushed out and spreads to the surrounding area.

2. The quantitative inoculation machine according to claim 1, characterized in that: The inoculation head (8) is provided with scale lines (10).

3. The quantitative inoculation machine according to claim 1, characterized in that: The outer wall of the outer sleeve (1501) is provided with an external thread, and the positioning block (14) is provided with an internal thread that matches the external thread.

4. The quantitative inoculation machine according to claim 1, characterized in that: The number of the spring limiting blocks (1503) is at least two.

5. The quantitative inoculation machine according to claim 1, characterized in that: The vertical distance between the spring limiting block (1503) and the bottom of the telescopic rod (1502) is greater than the depth of the slot (13).

6. The quantitative inoculation machine according to claim 1, characterized in that: The grip (2) is provided with a cable (7) that connects the solenoid valve (4) and the time relay (5).

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