A push-type tissue disrupter

Abstract

The utility model discloses a press type tissue breaker relates to biomedical field, it includes transparent container with holding handle and liquid level scale, and the top detachable installation of container is equipped with the sealing cover of sealing ring, the sealing cover is assembled soft tissue breaking mechanism, and this mechanism contains thread guide column (with limit boss), rotary sleeve (the outer wall is equipped with two -way / inclined / bottom vertical three -dimensional cutting blade), press handle and spring. Press handle drives rotary sleeve and rotates down along thread guide column, and blade cuts the organization and mixes homogeneously digestive juice, and after the hand is loose, spring drives sleeve reset, can cyclic operation. The device does not need electric power, and the structure is simple, and the operation is portable, and breaks even high -efficient, and adapts the demand of experiment.

Description

Technical Field

[0001] This utility model relates to the field of biomedicine, specifically to a press-type tissue disruptor. Background Technology

[0002] In the biomedical field, some experiments require the disruption of biological tissues, such as organoid culture and primary cell culture. Current techniques primarily involve using ophthalmic scissors, surgical scissors, or blades for tissue disruption. However, tissues are highly resilient, making shearing and disruption difficult, and the lack of dedicated biological tissue disruption equipment severely impacts the progress and speed of experiments. Utility Model Content

[0003] This utility model provides a press-type tissue disruptor, which is equipped with a transparent container containing a digestive fluid mixed with soft tissue. The top opening of the transparent container is detachably fitted with a sealing cap.

[0004] A soft tissue disruption mechanism is installed on the sealing cap. The soft tissue disruption mechanism includes:

[0005] A threaded guide post is fixed to the lower surface of the sealing cover. The outer surface of the threaded guide post is provided with external threads, and a limiting boss is provided at the bottom end of the threaded guide post.

[0006] Rotary sleeve, the rotary sleeve is a closed hollow sleeve, the top plate of the rotary sleeve is provided with a threaded hole that mates with the threaded guide post;

[0007] Press the handle, and the handle will slide longitudinally through the sealing cover and abut against the upper surface of the rotating sleeve top plate;

[0008] A spring is fitted onto the threaded guide post and located between the lower surface of the top plate of the rotating sleeve and the upper surface of the limiting boss.

[0009] A three-dimensional cutting blade has several metal blades that are staggered in both circumferential and axial directions on the outer surface of a rotating sleeve.

[0010] Furthermore, the sealing cap is longitudinally perforated with multiple guide holes that are arranged around the periphery of the threaded guide post;

[0011] The pressing handle is equipped with a pressing plate and multiple vertical pressure strips fixedly installed at the bottom of the pressing plate. The vertical pressure strips slide through the guide hole and have anti-detachment protrusions fixed at the bottom with a width greater than the inner diameter of the guide hole.

[0012] Furthermore, the three-dimensional cutting blade is provided with several forward blades and reverse blades, with the cutting edges of the forward blades and reverse blades facing opposite directions.

[0013] Furthermore, the three-dimensional cutting blade has several blades with upward-sloping ends and several blades with downward-sloping ends.

[0014] Furthermore, the bottom of the rotating sleeve is provided with several vertically downward-pointing blades.

[0015] Furthermore, a handle is provided on one side of the outer wall of the transparent container, and a liquid level scale is provided on the outer wall of the transparent container.

[0016] Furthermore, the sealing cap is equipped with a sealing ring that seals with the opening at the top of the transparent container.

[0017] The advantages of this utility model are:

[0018] 1) The crushing action can be driven by manual pressing. It has a simple structure, is highly portable, and is suitable for various laboratory operating environments.

[0019] 2) The threaded engagement between the threaded guide post and the rotating sleeve, combined with the automatic spring reset function, forms a cyclic action of "pressing-rotating descent-crushing-releasing-reverse reset", which can complete efficient crushing in a short time without repeated disassembly or adjustment.

[0020] 3) During the rotation of the three-dimensional cutting blade, the digestive fluid is driven to form a vortex, which makes the tissue fragments and digestive fluid mix quickly and thoroughly, reduces tissue aggregation, avoids the problem of insufficient digestion in the later stage, and greatly improves the efficiency of the experimental process.

[0021] 4) The sealing cap and transparent container are detachably connected, which facilitates the placement of tissue and digestion fluid in the early stage, the removal of samples in the later stage, and the cleaning of equipment; the core components have a simple structure, no complex transmission mechanism, and low maintenance cost. Attached Figure Description

[0022] To more clearly illustrate the technical solutions in the embodiments of this utility model, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0023] Figure 1 This is a simplified structural diagram of a press-type tissue disruptor according to this utility model;

[0024] Figure 2 This is a schematic diagram of the tissue disruptor of this utility model after the handle is pressed. Detailed Implementation

[0025] In the following description, numerous specific details are set forth in order to provide a more thorough understanding of the present invention. However, it will be apparent to those skilled in the art that the present invention can be practiced without one or more of these details. In other instances, certain technical features well-known in the art have not been described in order to avoid confusion with the present invention.

[0026] To fully understand this utility model, detailed steps and structures will be presented in the following description to illustrate the technical solution of this utility model. Preferred embodiments of this utility model are described in detail below; however, in addition to these detailed descriptions, this utility model may have other embodiments.

[0027] Reference Figure 1-2 As shown, this invention provides a press-type tissue disruptor that can quickly cut and break down biological tissues and thoroughly mix the tissue fragments with the digestion and dissociation fluid. This auxiliary device has a simple structure, is easy to use, optimizes experimental operations, and greatly accelerates the experimental speed. The specific solution is as follows:

[0028] The press-type tissue disruptor has a transparent container 100 containing a digestive fluid mixed with soft tissue. A removable sealing cap 110 is installed on the top opening of the transparent container 100. Liquid level markings are provided on the outer wall of the transparent container 100 for easy visual observation of the internal solution level.

[0029] A soft tissue crushing mechanism 200 is installed on the sealing cover 110. The soft tissue crushing mechanism 200 includes a threaded guide post 210, a rotating sleeve 220, a pressing handle 230, a spring 240, and a three-dimensional cutting blade 250.

[0030] A threaded guide post 210 is fixed to the lower surface of the sealing cover 110. The outer surface of the threaded guide post 210 has external threads, and a limiting boss 211 is provided at the bottom end of the threaded guide post 210. The rotating sleeve 220 is a closed hollow sleeve, and the top plate 221 of the rotating sleeve has a threaded hole that mates with the threaded guide post 210. The pressing handle 230 slides longitudinally through the sealing cover 110 and contacts the top plate 221 of the rotating sleeve. A spring 240 is sleeved on the threaded guide post 210 and located between the lower surface of the top plate 221 of the rotating sleeve and the upper surface of the limiting boss 211. The three-dimensional cutting blade 250 has several metal blades that are staggered circumferentially and axially distributed on the outer surface of the rotating sleeve 220.

[0031] In an optional embodiment, the sealing cap 110 has a plurality of guide holes 111 longitudinally extending through it and surrounding the threaded guide post 210; the pressing handle 230 has a pressing plate 231 and a plurality of vertical pressure strips 232 fixedly installed on the bottom of the pressing plate 231. The vertical pressure strips 232 slide through the guide holes 111 and have anti-detachment protrusions 233 at their bottom with a width greater than the inner diameter of the guide holes 111, thereby preventing the pressing handle 230 from detaching from the sealing cap 110. The pressing plate 231 has an arc-shaped convex surface to improve pressing comfort.

[0032] In an optional embodiment, the three-dimensional cutting blade 250 is provided with a plurality of forward and reverse blades, with the cutting edges of the forward and reverse blades facing opposite directions. The bidirectional blade design utilizes the bidirectional stroke of the rotating sleeve to achieve a dual fragmentation effect of "downward cutting + resetting back cutting". The forward blade applies a forward shearing force when the sleeve rotates downward, while the reverse blade performs a secondary cut on the incompletely fragmented tissue when the sleeve rotates upward to reset, avoiding tissue slippage due to unidirectional force and improving cutting efficiency; the bidirectional blades form a cross-shearing action, breaking down the toughness and resistance of biological tissue, making the tissue fragments smaller and more uniform, reducing the residue of large tissue pieces, creating conditions for sufficient contact between the digestive fluid and the tissue, and adapting to the tissue fragmentation precision requirements of biomedical experiments.

[0033] In an optional embodiment, the three-dimensional cutting blade 250 is provided with a plurality of blades 251 with upwardly inclined ends and a plurality of blades 252 with downwardly inclined ends. The upwardly and downwardly inclined blades form a three-dimensional cutting space. When the sleeve is pressed down, the blades with downwardly inclined ends penetrate deep into the tissue, actively "slicing" deep tissue; the blades with upwardly inclined ends "hook" and slice upper and suspended tissue blocks when rotating, comprehensively covering tissues at different heights and positions within the container. At the same time, the inclined blades generate oblique shearing force when in contact with the tissue, which is more effective at cutting through tough tissues than vertical blades. When rotating, the blades drive the digestive fluid to form a vortex, promoting rapid mixing of tissue fragments and digestive fluid, reducing tissue aggregation, and improving subsequent dissociation efficiency.

[0034] In an optional embodiment, a plurality of vertically downward-pointing blades 253 are fixed to the bottom of the rotating sleeve 220. Addressing the issue of biological tissues easily depositing at the bottom of the container due to gravity, the vertical blades can cover the bottom cutting blind zone. When the sleeve rotates and presses down, it directly inserts into the bottom tissue clumps, breaking up and chopping the deposited tissue, avoiding "bottom tissue aggregation without breaking down." Simultaneously, the vertically downward-pointing blades can vertically puncture and cut, supplementing the circumferential and axial cutting dimensions, ensuring uniform tissue fragmentation from the surface to the middle and bottom layers. This ensures consistent fragment size, meeting the requirements for tissue fragmentation uniformity in experiments such as organoid and primary cell culture, reducing incomplete digestion caused by tissue deposition, and improving experimental efficiency.

[0035] In an optional embodiment, after the handle 230 is pressed down to its maximum stroke, the top plate 221 of the rotating sleeve remains above the liquid level in the container, and the vertical blade 253 is kept at a distance from the bottom of the transparent container 100. This prevents the solution from entering the rotating sleeve 220 and affecting its rotation, as well as from corroding the spring 240; it also prevents the three-dimensional cutting blade 250 from touching the bottom and damaging the container.

[0036] In an optional embodiment, a handle 120 is provided on one outer wall of the transparent container 100. During operation, the operator holds the handle 120 with one hand to fix the transparent container 100, and presses down on the pressing plate 231 of the pressing handle 230 with the other hand. The vertical pressure bar 232 slides downward and applies downward pressure to the top plate 221 of the rotating sleeve. Since the rotating sleeve 220 is threadedly connected to the threaded guide post 210, the rotating sleeve 220 rotates downward. At the same time, the three-dimensional cutting blade 250 fixed on the rotating sleeve 220 cuts and breaks up the tissue in the solution and mixes the tissue fragments with the digestive fluid. After releasing the handle, the rotating sleeve 220 rotates upward and returns to its original position under the action of the spring 240, preparing for the next downward pressing and breaking.

[0037] This invention relates to a manual device specifically designed for rapid mechanical disruption of soft tissues in biomedical experiments (such as organoid culture and primary cell culture). Its core working principle is based on a cyclical action of "pressing → forced rotation and descent via a threaded mechanism → multi-directional cutting by a three-dimensional blade → automatic spring reset," as detailed below:

[0038] 1) Initial state: The transparent container is pre-filled with digestive fluid containing soft tissue, and the sealed cap is closed. The internal rotating sleeve is connected to the fixed threaded guide post through the thread. The spring is in a compressed or slightly compressed state, and the three-dimensional cutting blade is suspended above the liquid surface or shallowly immersed in the liquid.

[0039] 2) Pressing Stage (Crushing Action): The operator holds the container handle with one hand to stabilize it, and presses down on the handle (pressing plate) with the other hand. The pressing handle transmits force directly to the top plate of the rotating sleeve through multiple vertical pressure bars. Since the rotating sleeve can only rotate and descend along the threaded guide post (forced guidance effect of the threaded pair), the vertical component of the pressing force causes the rotating sleeve to rotate and move downward simultaneously. The three-dimensional cutting blade fixed to the outer surface of the rotating sleeve rotates accordingly and penetrates into the tissue digestion fluid.

[0040] 3) Reset phase: After releasing the pressing handle, the spring on the threaded guide post releases its elastic potential energy, pushes the rotating sleeve upward, and returns to the initial position or close to the initial position, preparing for the next pressing.

[0041] 4) Repeated operation: Press and release repeatedly several times to quickly break down and mix the tissue. The whole process requires no electricity, is completely manual, and has a simple structure.

[0042] The preferred embodiments of this utility model have been described above. It should be understood that this utility model is not limited to the specific embodiments described above. Devices and structures not described in detail herein should be understood as being implemented in a conventional manner within the art. Any person skilled in the art can make many possible variations and modifications to the technical solutions of this utility model using the disclosed methods and techniques, or modify them into equivalent embodiments with equivalent changes, without departing from the scope of the technical solution of this utility model. This does not affect the essential content of this utility model. Therefore, any simple modifications, equivalent changes, and modifications made to the above embodiments based on the technical essence of this utility model, without departing from the content of the technical solution of this utility model, still fall within the protection scope of the technical solution of this utility model.

Claims

1. A pressure-type tissue disruptor, characterized in that, The press-type tissue disruptor is equipped with a transparent container containing a digestive fluid mixed with soft tissue. The top opening of the transparent container is detachably fitted with a sealing cap. A soft tissue disruption mechanism is installed on the sealing cap. The soft tissue disruption mechanism includes: A threaded guide post is fixed to the lower surface of the sealing cover. The outer surface of the threaded guide post is provided with external threads, and a limiting boss is provided at the bottom end of the threaded guide post. Rotary sleeve, the rotary sleeve is a closed hollow sleeve, the top plate of the rotary sleeve is provided with a threaded hole that mates with the threaded guide post; Press the handle, and the handle will slide longitudinally through the sealing cover and abut against the upper surface of the rotating sleeve top plate; A spring is fitted onto the threaded guide post and located between the lower surface of the top plate of the rotating sleeve and the upper surface of the limiting boss. A three-dimensional cutting blade has several metal blades that are staggered in both circumferential and axial directions on the outer surface of a rotating sleeve.

2. The press-type tissue disruptor as described in claim 1, characterized in that, The sealing cap has multiple guide holes that run longitudinally through the periphery of the threaded guide post; The pressing handle is equipped with a pressing plate and multiple vertical pressure strips fixedly installed at the bottom of the pressing plate. The vertical pressure strips slide through the guide hole and have anti-detachment protrusions fixed at the bottom with a width greater than the inner diameter of the guide hole.

3. A press-type tissue disruptor as described in claim 1, characterized in that, The three-dimensional cutting blade has several forward blades and reverse blades, with the cutting edges of the forward blades and reverse blades facing opposite directions.

4. A press-type tissue disruptor as described in claim 1, characterized in that, The three-dimensional cutting blade has several blades with upward-sloping ends and several blades with downward-sloping ends.

5. A press-type tissue disruptor as described in claim 1, characterized in that, The bottom of the rotating sleeve is equipped with several vertically downward blades.

6. A pressure-type tissue disruptor as described in claim 1, characterized in that, The transparent container has a handle on one side of its outer wall, and a liquid level scale is also provided on the outer wall of the transparent container.

7. A press-type tissue disruptor as described in claim 1, characterized in that, The sealing cap is equipped with a sealing ring that seals with the opening at the top of the transparent container.

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