A pathogen detection device based on nano-probe and micro-fluidic chip

Abstract

The utility model relates to the technical field of pathogen detection based on nanometer probe and micro -fluidic chip, concretely relates to a pathogen detection device based on nanometer probe and micro -fluidic chip, including pcr detection instrument body, the pcr detection instrument body includes apron, tray, cavity and hot lid. The utility model after closing apron, through ninety degree rotation pivot, through cam to push hot lid to slide, and compress first spring to fixedly place the test tube on the tray to press down, prevent when detecting, test tube positioning is not unstable, leads to the deviation of detection structure, better fixed effect to test tube, when pushing hot lid, first pull slide, and compress second spring, make that pin rod slide out pin hole, through the slide after ninety degree rotation pivot, pin rod inserts corresponding pin hole, realize the effect of pivot limit stop, in this way, can avoid the situation of hot lid displacement because of equipment shaking when detecting, further guarantee the position fixed of hot lid.

Description

Technical Field

[0001] This utility model relates to the field of pathogen detection technology based on nanoprobes and microfluidic chips, specifically a pathogen detection device based on nanoprobes and microfluidic chips. Background Technology

[0002] Pathogen detection devices based on nanoprobes and microfluidic chips are commonly referred to as lab-on-a-chip (Lab-on-a-Chip) devices. These devices integrate microfluidic technology, nanomaterials, and biosensing technology to achieve rapid sample detection and analysis. They often utilize PCR (polymerase chain reaction) instruments, which employ microfluidic chip technology to integrate sample processing, amplification reactions, and signal detection, supporting both dye-based and TaqMan probe-based detection modes. A multi-channel optical system simultaneously monitors fluorescence signals at different wavelengths, enabling parallel detection of multiple indicators in a single run.

[0003] Existing PCR testing instruments require placing the test tube, closing the lid, and then adjusting the heated cap downwards to fix the test tube in place. However, the heated cap adjustment does not provide a limiting effect, and during use, external vibrations and other factors can easily cause changes in the fit of the heated cap, affecting the accuracy of the test data.

[0004] Therefore, a pathogen detection device based on nanoprobes and microfluidic chips is proposed to solve the problems mentioned above. Utility Model Content

[0005] To address the shortcomings of existing technologies, this invention provides a pathogen detection device based on nanoprobes and microfluidic chips. This device solves the problem that existing PCR testing instruments, when used, require placing the test tube, closing the cover, and then adjusting the heated cap downwards to fix the test tube. However, the heated cap adjustment does not provide a limiting effect, and during use, external vibrations and other factors can easily alter the fit of the heated cap, affecting the accuracy of the test data.

[0006] To achieve the above objectives, this utility model provides the following technical solution: It includes a PCR testing instrument body, comprising a cover plate, a tray, a cavity, and a heated cover. The tray is fixedly connected to the top of the PCR testing instrument body. The cavity is disposed inside the cover plate. The heated cover is slidably connected inside the cavity. A sliding mechanism is provided between the cavity and the heated cover. The sliding mechanism includes a limiting groove, a first guide rod, a connecting frame, and a first spring. The limiting groove is formed inside the cavity. The first guide rod is fixedly connected inside the limiting groove. The connecting frame is slidably connected to the outside of the first guide rod. The first spring is sleeved on the outside of the first guide rod. A transmission mechanism is provided inside the cavity.

[0007] Preferably, the connecting frame has an L-shaped structure, and the outer wall of one end of the connecting frame is combined with the inner wall of the limiting groove.

[0008] Preferably, the transmission mechanism includes a rotating shaft, a cam, a fixed ring, a pin hole, a slide groove, a slide frame, a pin rod, a second guide rod, a second spring, and a handle. The rotating shaft is rotatably connected inside the cavity, the cam is fixedly connected to the outside of the rotating shaft, the fixed ring is fixedly connected to one side of the cover plate, the pin hole is opened on the outside of the fixed ring, the slide groove is opened inside one end of the rotating shaft, the slide frame is slidably connected inside the slide groove, the pin rod is fixedly connected to the outside of the slide frame, the second guide rod is fixedly connected to one side of the slide frame, the second spring is sleeved on the outside of the second guide rod, and the handle is fixedly connected to the outside of the slide frame.

[0009] Preferably, the rotating shaft passes through the rotating connecting ring, and the pin passes through the slidably connecting handle.

[0010] Preferably, the inner wall of the slide groove fits against the outer wall of the slide frame, and four pin holes are provided, which are evenly distributed.

[0011] Preferably, the pin is slidably inserted into the inside of the pin hole, and the inner wall of the pin hole is in contact with the outer wall of the pin.

[0012] Preferably, a limiting mechanism is provided on the outer side of the cover plate. The limiting mechanism includes a buckle, a protrusion, a slot, a slide plate, and an ejector block. The buckle is fixedly connected to the top of the PCR testing instrument body, the protrusion is fixedly connected to the outer side of the cover plate, the slot is opened inside the protrusion, the slide plate is slidably connected inside the slot, and the ejector block is fixedly connected to the outer side of the slide plate.

[0013] Preferably, the inner wall of the slot fits against the outer wall of the slide plate, and the buckle is engaged inside the slot.

[0014] Compared with existing technologies, this invention provides a pathogen detection device based on nanoprobes and microfluidic chips, which has the following advantages: 1. After the cover is closed, the rotating shaft is rotated 90 degrees, which pushes the hot cover to slide through the cam and compresses the first spring, thereby pressing down and fixing the test tubes placed on the tray. This prevents the test tubes from being unstable during testing, which could lead to deviations in the testing structure and achieve a better fixation effect on the test tubes.

[0015] 2. When pushing the hot cap, first pull the slide and compress the second spring, so that the pin slides out of the pin hole. After the slide rotates the shaft 90 degrees, the pin inserts into the corresponding pin hole, which achieves the effect of limiting and stopping the rotation of the shaft. In this way, during the test, the hot cap can be prevented from shifting due to equipment shaking, further ensuring the position of the hot cap is fixed and better limiting the test tube.

[0016] 3. After the cover is closed, the buckle is engaged inside the slot. When the cover needs to be opened, simply slide the slide plate and push the buckle out of the slot through the ejector block to open the cover. This makes the operation more convenient and the closed state of the cover is more stable. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the structure of this utility model; Figure 2 This is a schematic diagram of the cam position structure of this utility model; Figure 3 This is a schematic diagram of the carriage structure of this utility model; Figure 4 This is a schematic diagram of the skateboard structure of this utility model; Figure 5 This utility model Figure 2 Enlarged structural diagram at point A in the middle.

[0018] In the diagram: 1. PCR testing instrument body; 2. Cover plate; 3. Tray; 4. Cavity; 5. Heated cover; 6. Limiting groove; 7. First guide rod; 8. Connecting frame; 9. First spring; 10. Rotating shaft; 11. Cam; 12. Fixing ring; 13. Pin hole; 14. Slide groove; 15. Slide carriage; 16. Pin rod; 17. Second guide rod; 18. Second spring; 19. Handle; 20. Buckle; 21. Protrusion; 22. Slot; 23. Slide plate; 24. Ejector block. Detailed Implementation

[0019] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0020] Example: Please see Figure 1 - Figure 5This embodiment of a pathogen detection device based on nanoprobes and microfluidic chips includes a PCR detection instrument body 1. The PCR detection instrument body 1 includes a cover plate 2, a tray 3, a cavity 4, and a hot cover 5. The tray 3 is fixedly connected to the top of the PCR detection instrument body 1. The cavity 4 is disposed inside the cover plate 2. The hot cover 5 is slidably connected inside the cavity 4. A sliding mechanism is provided between the cavity 4 and the hot cover 5. The sliding mechanism includes a limiting groove 6, a first guide rod 7, a connecting frame 8, and a first spring 9. The limiting groove 6 is opened inside the cavity 4. The first guide rod 7 is fixedly connected inside the limiting groove 6. The connecting frame 8 is slidably connected to the outside of the first guide rod 7. The first spring 9 is sleeved on the outside of the first guide rod 7. A transmission mechanism is provided inside the cavity 4. The PCR detection instrument body 1 is equipped with pathogen detection experiments based on nanoprobes and microfluidic chips. The model of the PCR detection instrument body 1 can be: Bio-rad T100. The structure and working principle of this model are existing technologies and will not be described in detail here.

[0021] After the cover plate 2 is closed, the rotating shaft 10 is rotated 90 degrees, and the cam 11 pushes the hot cover 5 to slide and compress the first spring 9, thereby pressing down and fixing the test tubes placed on the tray 3 to prevent the test tubes from being unstable during testing, which would cause deviations in the testing structure and achieve a better fixing effect on the test tubes.

[0022] Please see Figure 1 - Figure 5 The connecting frame 8 has an L-shaped structure, and the outer wall of one end of the connecting frame 8 is combined with the inner wall of the limiting groove 6.

[0023] To better secure the hot cover 5, the transmission mechanism includes a rotating shaft 10, a cam 11, a retaining ring 12, a pin hole 13, a slide groove 14, a slide 15, a pin 16, a second guide rod 17, a second spring 18, and a handle 19. The rotating shaft 10 is rotatably connected inside the cavity 4. The cam 11 is fixedly connected to the outside of the rotating shaft 10. The retaining ring 12 is fixedly connected to one side of the cover plate 2. The pin hole 13 is opened on the outside of the retaining ring 12. The slide groove 14 is opened inside one end of the rotating shaft 10. The slide 15 is slidably connected inside the slide groove 14. The pin 16 is fixedly connected to the outside of the slide 15. The second guide rod 17 is fixedly connected to one side of the slide 15. The second spring 18 is sleeved on the outside of the second guide rod 17. The handle 19 is fixedly connected to the outside of the slide 15.

[0024] When pushing the hot cover 5, first pull the slide 15 and compress the second spring 18, so that the pin 16 slides out of the pin hole 13. After the slide 15 rotates the shaft 10 ninety degrees, release the slide 15, and the pin 16 of the slide 15 inserts into the corresponding pin hole 13, so as to achieve the effect of limiting and stopping the rotation of the shaft 10. In this way, during the test, the hot cover 5 can be prevented from shifting due to equipment shaking, further ensuring the fixed position of the hot cover 5 and better limiting the test tube.

[0025] Please see Figure 3 The rotating shaft 10 passes through and rotates to connect the fixed ring 12. The pin 16 slides through and connects to the handle 19. The inner wall of the slide groove 14 fits against the outer wall of the slide frame 15. There are four pin holes 13, which are equidistantly distributed. The pin 16 slides into the inside of the pin hole 13, and the inner wall of the pin hole 13 fits against the outer wall of the pin 16.

[0026] To facilitate opening and closing the cover plate 2, a limiting mechanism is provided on the outer side of the cover plate 2. The limiting mechanism includes a buckle 20, a protrusion 21, a slot 22, a slide plate 23, and an ejector block 24. The buckle 20 is fixedly connected to the top of the PCR testing instrument body 1, the protrusion 21 is fixedly connected to the outer side of the cover plate 2, the slot 22 is opened inside the protrusion 21, the slide plate 23 is slidably connected inside the slot 22, and the ejector block 24 is fixedly connected to the outer side of the slide plate 23.

[0027] After the cover plate 2 is closed, the buckle 20 is engaged inside the slot 22. When the cover plate 2 needs to be opened, simply slide the slide plate 23 and push the buckle 20 out of the slot 22 through the ejector block 24, thereby opening the cover plate 2. The operation is more convenient and the closed state of the cover plate 2 is more stable.

[0028] Please see Figure 4 The inner wall of the slot 22 fits against the outer wall of the slide plate 23, and the buckle 20 is engaged inside the slot 22.

[0029] The working principle of the above embodiments is as follows: After the cover plate 2 is closed, the rotating shaft 10 is rotated 90 degrees, and the cam 11 pushes the hot cover 5 to slide and compresses the first spring 9, thereby pressing down and fixing the test tubes placed on the tray 3 to prevent the test tubes from being unstable during testing, which would cause deviations in the testing structure and achieve a better fixing effect on the test tubes. When pushing the hot cover 5, first pull the slide 15 and compress the second spring 18, so that the pin 16 slides out of the pin hole 13. After the slide 15 rotates the shaft 10 ninety degrees, release the slide 15, and the pin 16 of the slide 15 inserts into the corresponding pin hole 13, so as to achieve the effect of limiting and stopping the rotation of the shaft 10. In this way, during the test, the hot cover 5 can be prevented from shifting due to equipment shaking, further ensuring the fixed position of the hot cover 5 and better limiting the test tube. After the cover plate 2 is closed, the buckle 20 is engaged inside the slot 22. When the cover plate 2 needs to be opened, simply slide the slide plate 23 and push the buckle 20 out of the slot 22 through the ejector block 24, thereby opening the cover plate 2. The operation is more convenient and the closed state of the cover plate 2 is more stable.

[0030] The installation, connection, or setting methods disclosed in this embodiment are all common mechanical connection methods. As long as they can achieve their beneficial effects, they can be implemented. Therefore, this embodiment will not elaborate on their specific structural composition and working principle.

[0031] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A pathogen detection device based on nanoprobes and microfluidic chips, characterized in that: The instrument includes a PCR testing instrument body (1), which includes a cover plate (2), a tray (3), a cavity (4), and a hot cover (5). The tray (3) is fixedly connected to the top of the PCR testing instrument body (1). The cavity (4) is located inside the cover plate (2). The hot cover (5) is slidably connected inside the cavity (4). A sliding mechanism is provided between the cavity (4) and the hot cover (5). The sliding mechanism includes a limiting groove (6), a first guide rod (7), a connecting frame (8), and a first spring (9). The limiting groove (6) is opened inside the cavity (4). The first guide rod (7) is fixedly connected inside the limiting groove (6). The connecting frame (8) is slidably connected to the outside of the first guide rod (7). The first spring (9) is sleeved on the outside of the first guide rod (7). A transmission mechanism is provided inside the cavity (4).

2. The pathogen detection device based on nanoprobes and microfluidic chips according to claim 1, characterized in that: The connecting frame (8) has an L-shaped structure, and the outer wall of one end of the connecting frame (8) is combined with the inner wall of the limiting groove (6).

3. The pathogen detection device based on nanoprobes and microfluidic chips according to claim 1, characterized in that: The transmission mechanism includes a rotating shaft (10), a cam (11), a fixed ring (12), a pin hole (13), a slide groove (14), a slide (15), a pin rod (16), a second guide rod (17), a second spring (18), and a handle (19). The rotating shaft (10) is rotatably connected to the inside of the cavity (4). The cam (11) is fixedly connected to the outside of the rotating shaft (10). The fixed ring (12) is fixedly connected to one side of the cover plate (2). The pin hole (13) is opened on the outside of the fixed ring (12). The slide groove (14) is opened inside one end of the rotating shaft (10). The slide (15) is slidably connected to the inside of the slide groove (14). The pin rod (16) is fixedly connected to the outside of the slide (15). The second guide rod (17) is fixedly connected to one side of the slide (15). The second spring (18) is sleeved on the outside of the second guide rod (17). The handle (19) is fixedly connected to the outside of the slide (15).

4. The pathogen detection device based on nanoprobes and microfluidic chips according to claim 3, characterized in that: The rotating shaft (10) passes through the rotating connection fixing ring (12), and the pin (16) slides through the handle (19).

5. A pathogen detection device based on nanoprobes and microfluidic chips according to claim 3, characterized in that: The inner wall of the slide groove (14) fits against the outer wall of the slide frame (15), and four pin holes (13) are provided, which are evenly distributed.

6. The pathogen detection device based on nanoprobes and microfluidic chips according to claim 3, characterized in that: The pin (16) is slidably inserted into the inside of the pin hole (13), and the inner wall of the pin hole (13) is in contact with the outer wall of the pin (16).

7. The pathogen detection device based on nanoprobes and microfluidic chips according to claim 1, characterized in that: The outer side of the cover plate (2) is provided with a limiting mechanism, which includes a buckle (20), a protrusion (21), a slot (22), a slide plate (23), and an ejector block (24). The buckle (20) is fixedly connected to the top of the PCR testing instrument body (1), the protrusion (21) is fixedly connected to the outer side of the cover plate (2), the slot (22) is opened inside the protrusion (21), the slide plate (23) is slidably connected inside the slot (22), and the ejector block (24) is fixedly connected to the outer side of the slide plate (23).

8. The pathogen detection device based on nanoprobes and microfluidic chips according to claim 7, characterized in that: The inner wall of the slot (22) fits against the outer wall of the slide plate (23), and the buckle (20) is engaged inside the slot (22).

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