An improved cell counting plate for sperm count

Abstract

The utility model belongs to the field of biotechnology, specifically disclose a kind of cell counting plate for improvement for sperm counting, to solve the problem of counting error caused by uneven sample flow and cover glass positioning deviation;Specifically including glass slide matrix, for realizing sperm quantitative counting counting chamber, the cover glass of adaptation covering above glass slide matrix and the support column for supporting cover glass to form counting gap, the liquid-adding side of counting chamber is integrally formed with connecting groove and spherical groove;Spherical groove is located at the end of connecting groove away from counting chamber, and its bottom is flush with the bottom of connecting groove;On the one hand, through the flow guide structure of spherical groove and connecting groove, the problem of uneven sample distribution caused by surface tension is solved, and the counting accuracy is improved;On the other hand, through the positioning of fitting groove and the design of multiple counting chambers, the operation difficulty is reduced and the batch detection requirement is adapted, the cost control and detection efficiency are considered, and it is suitable for the sperm quality detection scene of large-scale breeding industry.

Description

Technical Field

[0001] This invention relates to the field of biotechnology, and in particular to an improved cell counting board for sperm counting. Background Technology

[0002] With the large-scale application of artificial insemination technology in the livestock industry, the accuracy of sperm counting directly affects breeding efficiency, and the demand for counting accuracy is becoming increasingly urgent in scenarios such as boar stations and bull stations. Currently, the mainstream counting tools in the industry are mainly divided into two categories: one is the hemocytometer, which is widely used in teaching and research due to its reusability and low cost, but it suffers from significant counting errors; the other is the commercial semen counting instrument, which, although compatible with computer-aided analysis, is expensive and also does not solve the core source of error.

[0003] The common drawback of the aforementioned counting chambers lies in their failure to effectively address the uneven sample distribution caused by surface tension: In routine operation, 10-20 microliters of diluted semen need to be added. Due to the high viscosity of semen, the liquid easily forms spherical hemispheres on the counting chamber surface due to surface tension. If the counting chamber is naturally drawn in by siphoning, it will cause significant differences in sperm distribution across different fields of view, making it difficult to reflect the true sample concentration even when using the multi-field arithmetic mean method. Furthermore, existing counting chambers lack a sample buffer structure, making it easy for excessive addition of semen to cause spillage, further affecting counting accuracy. Simultaneously, misalignment of the coverslip positioning can lead to unstable counting gaps, causing sperm overlap or stratification, increasing counting errors.

[0004] To address the aforementioned technical shortcomings, a solution is proposed that can achieve uniform sample flow and precise positioning through structural optimization, thereby improving the accuracy and ease of operation of sperm counting. Utility Model Content

[0005] The purpose of this invention is to provide an improved cell counting board for sperm counting to solve the problems mentioned above.

[0006] The objective of this invention can be achieved through the following technical solution: an improved cell counting plate for sperm counting, comprising a slide substrate, a counting chamber for quantitative sperm counting, a coverslip adapted to cover the slide substrate, and a support column for supporting the coverslip to form a counting gap; the liquid-filling side of the counting chamber is integrally formed with a connecting groove and a spherical groove; the bottom of the connecting groove is flush with the plane of the counting chamber, and one end of the connecting groove is connected to the counting chamber, and the other end is connected to the spherical groove; the spherical groove is located at the end of the connecting groove away from the counting chamber, and its bottom is flush with the bottom of the connecting groove; the spherical groove is used to temporarily store the sperm sample to be counted, and guides the sample into the counting chamber through the connecting groove.

[0007] Preferably, the upper surface of the slide substrate is provided with a fitting groove for accurately positioning and placing the cover glass, the depth of the fitting groove is adapted to the thickness of the cover glass, and a confluence groove is provided on the surface of the slide substrate corresponding to the outer area of ​​the counting chamber.

[0008] Preferably, the manifold has an annular structure and a smooth chamfer is provided at the inner diameter edge.

[0009] Preferably, the width of the connecting groove is set to 0.5-1.0 mm, and the length is set to 1-3 mm.

[0010] Preferably, the diameter of the spherical groove is set to 2-4 mm, and the volume of the spherical groove corresponding to this diameter can be adapted to a sample dropping volume of 10-80 microliters.

[0011] Preferably, there are two counting chambers, which are symmetrically distributed on the slide substrate; each counting chamber is independently equipped with a set of connecting grooves and spherical grooves, and the structure and size of each set of connecting grooves and spherical grooves are consistent.

[0012] The beneficial effects of this utility model are: This invention achieves both sample buffering and precise flow guidance through a combination of a spherical groove and a connecting groove. The spherical groove can temporarily hold 10-80 microliters of sample, avoiding spillage caused by excessive dripping. At the same time, the gradient flow guidance effect of the connecting groove breaks the hemispherical structure formed by the surface tension of semen, allowing the sample to fill the counting chamber evenly and solving the counting deviation caused by uneven sperm distribution. The design of the support column flush with the plane of the counting chamber and the connecting groove ensures stable and uniform counting gaps, reduces sperm overlap, and improves imaging clarity.

[0013] This invention achieves precise positioning of the coverslip through a fitting groove, the depth of which matches the thickness of the coverslip, avoiding offset errors during manual placement. Combined with the smooth chamfered structure of the annular confluence groove, it can collect overflow liquid and reduce the generation of air bubbles, further ensuring a stable counting environment. The symmetrical distribution design of the two independent counting chambers supports simultaneous detection of multiple samples or repeated verification of the same sample, balancing detection efficiency and result reliability, and adapting to the batch detection needs of large-scale aquaculture scenarios. Attached Figure Description

[0014] The present invention will be further described below with reference to the accompanying drawings; Figure 1 This is a schematic diagram of the overall structure of this utility model; Figure 2 This is a schematic diagram of the left side of the glass slide substrate structure of this utility model; Figure 3 This is a front view schematic diagram of the glass slide substrate structure of this utility model.

[0015] Legend: 1. Slide substrate; 2. Counting chamber; 3. Support column; 4. Cover glass; 5. Connecting groove; 6. Spherical groove; 11. Fitting groove; 12. Manifold. Detailed Implementation

[0016] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0017] Example 1: The application background of this utility model is based on the sperm concentration detection scenario in large-scale breeding farms. The existing detection relies on manual sample addition and counting, which is prone to inaccurate results due to operational deviations. The technical solution of this utility model is to solve the counting error problem caused by uneven sample diversion and cover glass positioning deviation. Please see Figure 1 - Figure 3 This embodiment is an improved cell counting board for sperm counting, including a slide substrate 1, a counting chamber 2 for quantitative sperm counting, a coverslip 4 adapted to cover the slide substrate 1, and a support column 3 for supporting the coverslip 4 to form a counting gap. The liquid-filling side of the counting chamber 2 is integrally formed with a connecting groove 5 and a spherical groove 6. The bottom of the connecting groove 5 is flush with the plane of the counting chamber 2. The width of the connecting groove 5 is set to 0.5-1.0 mm, the length is set to 1-3 mm, and one end of the connecting groove 5 is connected to the counting chamber 2, and the other end is connected to the spherical groove 6. The spherical groove 6 is located at the end of the connecting groove 5 away from the counting chamber 2, and its bottom is flush with the bottom of the connecting groove 5. The diameter of the spherical groove 6 is set to 2-4 mm. The volume of the spherical groove 6 corresponding to this diameter can be adapted to a sample drop volume of 10-80 microliters. The spherical groove 6 is used to temporarily store the sperm sample to be counted and guide the sample into the counting chamber 2 through the connecting groove 5. The upper surface of the slide substrate 1 is provided with a fitting groove 11 for accurately positioning and placing the cover glass 4. The depth of the fitting groove 11 is adapted to the thickness of the cover glass 4. A confluence groove 12 is provided on the surface of the slide substrate 1 corresponding to the outer area of ​​the counting chamber 2. The confluence groove 12 has a ring structure and a smooth chamfer is provided at the inner diameter edge.

[0018] Reference Figure 1 - Figure 3An improved cell counting plate for sperm counting includes a slide substrate 1, two symmetrically distributed counting chambers 2, a support column 3 and a cover glass 4. Each counting chamber 2 has an integrally formed connecting groove 5 and a spherical groove 6 on the liquid addition side. The connecting groove 5 is 0.8 mm wide and 2 mm long, and the spherical groove 6 is 3 mm in diameter to accommodate a conventional sample volume of 30-50 microliters. The upper surface of the slide substrate 1 has a fitting groove 11 with a depth consistent with the thickness of the coverslip 4. The inner edge of the annular confluence groove 12 in the middle has a smooth chamfer of R1.5mm. In use, the diluted semen sample is dropped into the spherical groove 6. Under the action of gravity and capillary action, the sample flows into the counting chamber 2 at a uniform speed through the connecting groove 5, avoiding the accumulation of liquid caused by direct drop. After the coverslip 4 is embedded in the fitting groove 11, the support column 3 ensures that the counting gap is constant, reducing the phenomenon of sperm stratification. This structure allows the sample to form a uniform single layer distribution within the counting chamber 5, enabling clear differentiation of individual sperm cells when observed under a microscope, thus avoiding counting errors caused by differences in aggregation or distribution.

[0019] Example 2: Please refer to Figure 1 - Figure 3 The two counting chambers 2 are independently configured with connecting slots 5 and spherical slots 6, and the structural dimensions of each group are consistent; In the batch testing scenario at the boar station, the operator can process two semen samples simultaneously: different samples are dripped into their respective spherical grooves 6. Thanks to the buffer capacity of the spherical grooves 6, even if the dripping volume deviates by as much as 60 microliters, no spillage will occur; the manifold 12 can collect excess liquid overflowing from the edge of the coverslip 4, avoiding cross-contamination between samples; during testing, the results of the two counting chambers can be compared to eliminate abnormal data and improve accuracy. For frozen and revived semen with high viscosity, the temporary storage space in the spherical groove 6 can reserve sample diffusion time. Combined with gentle tapping of the coverslip to eliminate protein adhesion, it further ensures uniform sperm dispersion and solves the problem of easy aggregation of viscous samples in traditional counting chambers.

[0020] As can be seen from Embodiments 1 and 2, on the one hand, the flow guiding structure of the spherical groove 6 and the connecting groove 5 solves the problem of uneven sample distribution caused by surface tension and improves counting accuracy; on the other hand, the positioning of the fitting groove 11 and the design of the multiple counting chambers 2 reduce the difficulty of operation and adapt to the needs of batch testing, taking into account both cost control and testing efficiency, and is suitable for sperm quality testing scenarios in large-scale aquaculture.

[0021] The above description is merely an example and illustration of the structure of this utility model. Those skilled in the art can make various modifications or additions to the specific embodiments described or use similar methods to replace them, as long as they do not deviate from the structure of the utility model or exceed the scope defined in the claims, they should all fall within the protection scope of this utility model.

Claims

1. An improved cell counting plate for sperm counting, comprising a slide substrate (1), a counting chamber (2) for realizing quantitative sperm counting, a coverslip (4) adapted to cover the slide substrate (1), and a support column (3) for supporting the coverslip (4) to form a counting gap, characterized in that: The counting chamber (2) has a connecting groove (5) and a spherical groove (6) integrally formed on the liquid-filling side; the bottom of the connecting groove (5) is flush with the plane of the counting chamber (2), and one end of the connecting groove (5) is connected to the counting chamber (2), and the other end is connected to the spherical groove (6); the spherical groove (6) is located at the end of the connecting groove (5) away from the counting chamber (2), and its bottom is flush with the bottom of the connecting groove (5). The spherical groove (6) is used to temporarily store the sperm sample to be counted, and guide the sample into the counting chamber (2) through the connecting groove (5).

2. The improved cell counting board for sperm counting according to claim 1, characterized in that: The upper surface of the slide substrate (1) is provided with a fitting groove (11) for accurately positioning and placing the cover glass (4). The depth of the fitting groove (11) is adapted to the thickness of the cover glass (4), and a confluence groove (12) is provided on the surface of the slide substrate (1) corresponding to the outer area of ​​the counting chamber (2).

3. The improved cell counting board for sperm counting according to claim 2, characterized in that: The manifold (12) has an annular structure and a smooth chamfer is provided at the edge of its inner diameter.

4. The improved cell counting board for sperm counting according to claim 1, characterized in that: The width of the connecting groove (5) is set to 0.5-1.0 mm, and the length is set to 1-3 mm.

5. An improved cell counting board for sperm counting according to claim 1, characterized in that: The diameter of the spherical groove (6) is set to 2-4 mm, and the volume of the spherical groove (6) corresponding to this diameter can be adapted to a sample drop volume of 10-80 microliters.

6. An improved cell counting board for sperm counting according to claim 1, characterized in that: There are two counting chambers (2), and the two counting chambers (2) are symmetrically distributed on the slide substrate (1); each counting chamber (2) is independently equipped with a set of connecting grooves (5) and spherical grooves (6), and the structure and size of each set of connecting grooves (5) and spherical grooves (6) are consistent.

Images