Exosome rapid separation and purification device

Abstract

The utility model discloses a kind of exosome rapid separation and purification device, including injection support, the sample storage tank is fixedly arranged on the upper end of injection support, control structure is provided above the sample storage tank and the limit seat is provided on the side of sample storage tank, the centrifugal separation cylinder is rotatably installed in the separation tank cut separation tank upper portion and is provided, the filter membrane is fixedly connected in the centrifugal separation cylinder, the liquid outlet pipe is fixedly arranged on the outer wall of separation tank, and the filter is fixedly installed in one end of liquid outlet pipe, two ultrafiltration membranes are fixedly installed in the filter, the liquid outlet pipe extension end is fixedly connected in the inside of purification support, a plurality of chromatographic columns are fixedly arranged in the inside of purification support, the collection tube is fixedly arranged on the outer wall of purification support, and the collection tube is inserted in the inside of collection container in one end, PLC controller is fixedly installed on the outer wall of injection support, can realize effectively reduce manual intervention, reduce the operation difficulty, improve the efficiency of separation and purification.

Description

Technical Field

[0001] This utility model relates to the field of biological separation and purification technology, and in particular to a device for rapid separation and purification of exosomes. Background Technology

[0002] Exosomes are nanoscale vesicles secreted by cells and are widely found in biological fluids such as blood, urine, and saliva. They contain bioactive substances such as proteins and nucleic acids and have important research value and application prospects in intercellular communication, disease diagnosis and treatment. Isolation and purification of elh exosomes is a prerequisite for carrying out related research and applications.

[0003] Existing methods for the separation and purification of exosomes have some shortcomings. Traditionally, methods such as density gradient centrifugation and immunoaffinity assays are mostly used. Although these methods have high specificity, they are expensive and can easily damage the activity of exosomes, which is not conducive to the effective separation of exosomes. Moreover, most separation and purification devices are complex in structure, have low automation, and low separation and purification efficiency, making it difficult to meet the demand for rapid acquisition of high-purity and high-activity exosomes. Therefore, a rapid exosome separation and purification device is proposed to solve the above problems. Utility Model Content

[0004] The purpose of this invention is to provide a rapid exosome separation and purification device to solve the problems mentioned in the background art.

[0005] To address the aforementioned problems, the following technical solution is provided: a rapid exosome separation and purification device, comprising an injection support, a sample storage tank fixedly mounted on the upper end of the injection support, a control structure above the sample storage tank and a limiting seat on one side of the sample storage tank, a separation tank above the limiting seat, a centrifuge cylinder rotatably mounted inside the separation tank, a filter membrane fixedly snapped into the centrifuge cylinder, an outlet pipe fixedly mounted on the outer wall of the separation tank and a filter fixedly mounted at one end of the outlet pipe, two ultrafiltration membranes fixedly mounted inside the filter, an extension end of the outlet pipe fixedly connected to the inside of the purification support, multiple chromatography columns fixedly mounted inside the purification support, and a collection tube fixedly mounted on the outer wall of the purification support and one end of the collection tube inserted into the inside of a collection container.

[0006] As a preferred embodiment of the above technical solution, a PLC controller is fixedly installed on the outer wall of the injection bracket, a drive motor is fixedly installed inside the limit seat, a main gear is fixedly connected to the output end of the drive motor, and a side gear is meshed with the outer wall of the main gear.

[0007] As a preferred embodiment of the above technical solution, a drive shaft is fixedly installed in the middle of the side gear, and one end of the drive shaft is fixedly installed at the bottom of the centrifugal separator.

[0008] As a preferred embodiment of the above technical solution, the outer wall of the filter is symmetrically threaded with two locking bolts, and a concentration sensor is fixedly installed on the outer wall of the purification bracket.

[0009] As a preferred embodiment of the above technical solution, the control structure includes a delivery pump body fixedly installed on the upper end of the sample storage tank. A triangular tube is fixedly installed at one end of the delivery pump body, and delivery pipes are fixedly connected to the other two ends of the triangular tube. One end of the delivery pipe is fixedly connected to the inside of the metering tank.

[0010] As a preferred embodiment of the above technical solution, the bottom of the metering tank is connected to the centrifugal separator, an electromagnetic control valve is fixedly installed on the outer wall of the conveying pipe, and a liquid level scale line is fixedly installed on the outer wall of the centrifugal separator.

[0011] Compared with the prior art, the beneficial effects of this utility model are as follows: 1. This utility model device is equipped with a sample injection tank, a separation mechanism, a purification mechanism, and a collection mechanism, and is automated by using a PLC controller. It realizes the integrated operation of exosomes from sample injection, separation, purification to collection, which simplifies the operation process, reduces manual intervention, lowers the operation difficulty, and improves the efficiency of separation and purification. By accurately controlling the centrifugation speed and time, and rationally selecting ultrafiltration membranes and filter membranes, the recovery rate of exosomes is greatly improved.

[0012] 2. The device of this utility model is equipped with a quantitative structure. By driving the delivery pump to start working, the sample is injected into the metering barrel through the delivery pipe. The liquid level is observed through the outer liquid level scale. When the set amount is reached, the electromagnetic control valve is closed to stop the delivery. Then the bottom of the metering barrel is opened to deliver it into the centrifuge separation cylinder. This facilitates quantitative control of the injected sample volume and avoids the problem of too much or too little sample being added manually, which would affect the exosome separation effect.

[0013] Specific embodiments of the present invention are disclosed in detail with reference to the following description and accompanying drawings, indicating how the principles of the present invention can be employed. It should be understood that the embodiments of the present invention are not limited in scope. Within the spirit and scope of the appended claims, the embodiments of the present invention include many changes, modifications, and equivalents. Attached Figure Description

[0014] The accompanying drawings are provided to further illustrate the present invention and form part of the specification. They are used together with the embodiments of the present invention to explain the present invention, but do not constitute a limitation thereof. In the drawings: Figure 1 This is a schematic diagram of the overall structure of a rapid exosome separation and purification device according to the present invention; Figure 2This is a partially disassembled structural diagram of a rapid exosome separation and purification device according to the present invention; Figure 3 for Figure 2 A partial enlarged diagram of the split structure; Figure 4 for Figure 1 A magnified schematic diagram of the local structure.

[0015] In the diagram: 1. Injection support; 2. PLC controller; 3. Sample storage tank; 4. Control structure; 41. Delivery pump; 42. Delivery pipeline; 43. Electromagnetic control valve; 44. Metering tank; 45. Liquid level scale; 46. Triangular tube; 5. Separation tank; 6. Limit seat; 61. Drive motor; 62. Main gear; 63. Side gear; 64. Drive shaft; 7. Discharge pipe; 8. Filter; 81. Ultrafiltration membrane; 82. Locking bolt; 9. Purification support; 10. Chromatography column; 11. Concentration sensor; 12. Collection container; 13. Centrifuge cylinder; 14. Filter membrane. Detailed Implementation

[0016] To make the technical means, creative features, objectives and effects of this utility model easier to understand, the present utility model will be further described below in conjunction with specific embodiments.

[0017] like Figures 1 to 4 As shown in the figure, this embodiment provides a rapid exosome separation and purification device, including an injection support 1, a sample storage tank 3 fixedly mounted on the upper end of the injection support 1, a control structure 4 above the sample storage tank 3 and a limiting seat 6 on one side of the sample storage tank 3, a separation tank 5 above the limiting seat 6, a centrifuge cylinder 13 rotatably mounted inside the separation tank 5, a filter membrane 14 fixedly snapped inside the centrifuge cylinder 13, an outlet pipe 7 fixedly mounted on the outer wall of the separation tank 5 and a filter 8 fixedly mounted at one end of the outlet pipe 7, two ultrafiltration membranes 81 fixedly mounted inside the filter 8, an extension end of the outlet pipe 7 fixedly connected to the inside of a purification support 9, multiple chromatography columns 10 fixedly mounted inside the purification support 9, a collection tube fixedly mounted on the outer wall of the purification support 9 and one end of the collection tube inserted into the inside of a collection container 12.

[0018] like Figures 3 to 4 As shown, a PLC controller 2 is fixedly installed on the outer wall of the injection bracket 1, a drive motor 61 is fixedly installed inside the limit seat 6, a main gear 62 is fixedly connected to the output end of the drive motor 61, a side gear 63 is meshed with the outer wall of the main gear 62, a drive shaft 64 is fixedly installed in the middle of the side gear 63, one end of the drive shaft 64 is fixedly installed at the bottom of the centrifuge cylinder 13, two locking bolts 82 are symmetrically threaded on the outer wall of the filter 8, and a concentration sensor 11 is fixedly installed on the outer wall of the purification bracket 9.

[0019] By setting up a filter membrane 14, which is detachably connected to the inner wall of the centrifuge cylinder 13 and located in the lower middle part of the centrifuge cylinder 13, when the centrifuge cylinder 13 rotates at high speed, impurities and large particles in the sample will move towards the inner wall of the centrifuge cylinder 13 under the action of centrifugal force and be blocked by the filter membrane 14. Exosomes, due to their small size, can enter the lower part of the centrifuge cylinder 13 through the pores of the filter membrane 14, achieving preliminary separation from impurities. At the same time, the filter 8 is fixed by two locking bolts 82, which is relatively easy to disassemble. The ultrafiltration membrane 81 can filter the liquid after centrifugation again to further remove any small particle impurities that may remain, ensuring that the liquid entering the chromatography column 10 is purer and improving the purification efficiency and effect of the chromatography column 10. The setting of the purification support 9 and the chromatography column 10 is a mature existing technology and has not been described in detail in this application.

[0020] like Figure 4 As shown, the control structure 4 includes a delivery pump body 41 fixedly installed on the upper end of the sample storage tank 3. A triangular tube 46 is fixedly installed at one end of the delivery pump body 41, and delivery pipes 42 are fixedly connected to the other two ends of the triangular tube 46. One end of the delivery pipe 42 is fixedly connected to the inside of the metering tank 44. The bottom of the metering tank 44 is interconnected with the centrifugal separation cylinder 13. An electromagnetic control valve 43 is fixedly installed on the outer wall of the delivery pipe 42. A liquid level scale line 45 is fixedly installed on the outer wall of the centrifugal separation cylinder 13.

[0021] By inserting one end of the delivery pipe 42 into the sample storage tank 3, the sample is pumped out by the delivery pump 41 and delivered to the metering tank 44. The liquid level is observed through the outer liquid level scale 45, which facilitates quantitative control of the delivered sample liquid volume and avoids problems such as poor separation effect caused by too much or too little liquid.

[0022] The working principle and process of this utility model are as follows: When the exosome rapid separation and purification device is working, the parameters of the delivery pump 41 and drive motor 61 are preset in advance by the PLC controller 2. The sample to be processed is placed into the sample storage tank 3 above the injection support 1. At this time, the delivery pump 41 starts to work, injecting the sample into the metering tank 44 through the delivery pipe 42. The liquid level is observed through the outer liquid level scale 45. When the set amount is reached, the electromagnetic control valve 43 is closed to stop the delivery. Then, the bottom of the metering tank 44 is opened to deliver the sample into the centrifuge cylinder 13, which facilitates quantitative control of the injected sample volume. The upper sealing cover is closed, and the drive motor 61 is started to drive the main gear 62 to rotate. The main gear 62 drives the drive shaft 64 on the side gear 63 to rotate, which in turn drives the centrifuge cylinder 13 to rotate at high speed. Under the action of centrifugal force... Impurities and large particles in the sample are blocked by the filter membrane 14. Exosomes pass through the filter membrane 14 into the lower part of the centrifuge cylinder 13. After separation, the inlet valve is opened and the centrifuged liquid enters the filter 8 through the outlet pipe 7. The liquid after centrifugation is filtered again by two ultrafiltration membranes 81 in the filter 8 to further remove any small particulate impurities that may remain, ensuring that the liquid entering the chromatography column 10 is purer. The agarose gel microspheres in the chromatography column 10 specifically adsorb the exosomes and then elute them with the eluent. The concentration sensor 11 monitors the concentration of exosomes in the eluent in real time and transmits the information to the controller. The eluent containing exosomes is transported into the collection container 12 through the collection tube. The whole process realizes automated and rapid separation and purification, improving the separation and purification efficiency and quality of exosomes.

[0023] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installed," "equipped with," "connected," etc., should be interpreted broadly. For example, "connection" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium; it can be a connection within two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0024] In the description of this embodiment, the terms "upper," "lower," "right," etc., refer to the orientation or positional relationship shown in the accompanying drawings. They are used only for ease of description and simplification of operation, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model. In addition, the terms "first" and "second" are only used for distinction in description and have no special meaning.

Claims

1. A rapid exosome separation and purification device, characterized in that, The system includes an injection support (1), a sample storage tank (3) is fixedly installed on the upper end of the injection support (1), a control structure (4) is provided above the sample storage tank (3), and a limiting seat (6) is provided on one side of the sample storage tank (3). A separation tank (5) is provided above the limiting seat (6), and a centrifugal separation cylinder (13) is rotatably installed inside the separation tank (5). A filter membrane (14) is fixedly snapped inside the centrifugal separation cylinder (13). An outlet pipe (7) is fixedly installed on the outer wall of the separation tank (5), and a filter (8) is fixedly installed at one end of the outlet pipe (7). Two ultrafiltration membranes (81) are fixedly installed inside the filter (8). The extension end of the outlet pipe (7) is fixedly connected to the inside of a purification support (9). Multiple chromatography columns (10) are fixedly installed inside the purification support (9). A collection tube is fixedly installed on the outer wall of the purification support (9), and one end of the collection tube is inserted into the inside of a collection container (12).

2. The rapid exosome separation and purification device according to claim 1, characterized in that, A PLC controller (2) is fixedly installed on the outer wall of the injection bracket (1), and a drive motor (61) is fixedly installed inside the limit seat (6). A main gear (62) is fixedly connected to the output end of the drive motor (61), and a side gear (63) is meshed with the outer wall of the main gear (62).

3. The rapid exosome separation and purification device according to claim 2, characterized in that, A drive shaft (64) is fixedly installed in the middle of the side gear (63), and one end of the drive shaft (64) is fixedly installed at the bottom of the centrifugal separator (13).

4. The rapid exosome separation and purification device according to claim 3, characterized in that, The filter (8) has two locking bolts (82) symmetrically threaded on its outer wall, and the purification bracket (9) has a concentration sensor (11) fixedly installed on its outer wall.

5. The rapid exosome separation and purification device according to claim 1, characterized in that, The control structure (4) includes a delivery pump body (41) fixedly installed on the upper end of the sample storage tank (3). A triangular tube (46) is fixedly installed on one end of the delivery pump body (41), and delivery pipes (42) are fixedly connected to the other two ends of the triangular tube (46). One end of the delivery pipe (42) is fixedly connected to the inside of the metering barrel (44).

6. The rapid exosome separation and purification device according to claim 5, characterized in that, The bottom of the metering barrel (44) is connected to the centrifugal separator (13), and an electromagnetic control valve (43) is fixedly installed on the outer wall of the conveying pipe (42). A liquid level scale line (45) is fixedly installed on the outer wall of the centrifugal separator (13).

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